不同浓度乙醇提取丹参中12种成分比较研究

开放科学（资源服务）标识码（OSID）：
Comparative Study on Extraction of 12 Components from Salviae Miltiorrhizae Radix et Rhizoma with Different Concentrations of Ethanol
2019 年 9 月第 26 卷第 9 期
பைடு நூலகம்
中国中医药信息杂志
·89·
不同浓度乙醇提取丹参中 12 种成分比较研究
孙锦，林力，彭勍，张鹏，任钧国，刘建勋
中国中医科学院西苑医院基础医学研究所中药药理北京市重点实验室，北京 100091
摘要：目的 建立 HPLC 同时测定丹参中 9 种水溶性成分和 3 种脂溶性成分含量的方法，以不同浓度乙
95 min 内，丹参中 12 种成分可实现完全分离，在线性范围内质量浓度与峰面积具有良好的线性关系（r＞0.998），
平均回收率为 95.28%～104.97%，RSD＜4.58%。不同浓度乙醇对丹参中 12 种成分含量影响不同，随溶剂极性
降低，水溶性成分总量呈先升高后降低的趋势，脂溶性成分持续升高。结论 本研究建立的方法专属性好，灵
Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China Abstract: Objective To establish an HPLC method for the simultaneous determination of nine hydrophilic compounds and three lipophilic compounds in Salviae Miltiorrhizae Radix et Rhizoma; To discuss the effects of solvent polarity difference on the dissolution of active components in Salviae Miltiorrhizae Radix et Rhizoma represented by different concentrations of ethanol. Methods The HPLC separation was performed on an Agilent Extend-C18 column (4.6 mm × 250 mm, 5 μm), eluted with the mobile phase of acetonitrile-0.1% phosphoric acid solution. The flow rate was 0.8 mL/min; the column temperature was 25 ℃; the detection wavelength was 280 nm; the injection volume was 10 μL. The contents of 12 components in Salviae Miltiorrhizae Radix et Rhizoma extracted from different concentrations of ethanol (0%, 20%, 40%, 60%, 80%, 95%) were investigated. Results The complete separation was obtained within 95 minutes for the 12 components. There was a good linear relationship between concentration and peak area in the linear range (r>0.998). The average recoveries were 95.28%–104.97% and RSD were less than 4.58%. Different concentrations of ethanol had different influence on the contents of 12 components. The total amount of water-soluble components increased first and then decreased with the decrease of the polarity of the solvent, and the fat-soluble component continued to rise. Conclusion The method has good specificity, high sensitivity and accurate quantitative, which can be used for simultaneous determination of nine hydrophilic compounds and three lipophilic compounds in Salviae Miltiorrhizae Radix et Rhizoma, and different concentrations of ethanol have great influence on the dissolution of the 12 components. Keywords: Salviae Miltiorrhizae Radix et Rhizoma; concentrations of ethanol; water-soluble components; fat-soluble components
SUN Jin, LIN Li, PENG Qing, ZHANG Peng, REN Junguo, LIU Jianxun Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Institute of Basic Medical Sciences of Xiyuan
敏度高，定量准确，可用于同时测定丹参中 9 种水溶性成分和 3 种脂溶性成分的含量。不同浓度乙醇对丹参中
12 种指标性成分的溶出有很大影响。
关键词：丹参；乙醇浓度；水溶性成分；脂溶性成分
中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2019)09-0089-05
DOI：10.3969/j.issn.1005-5304.2019.09.020
醇为代表，探讨溶剂极性对丹参有效成分溶出的影响。方法 采用 Agilent Extend-C18 色谱柱（4.6 mm×250 mm，
5 μm），以乙腈-0.1%磷酸溶液为流动相，梯度洗脱，流速 0.8 mL/min，柱温 25 ℃，检测波长 280 nm，进样量
10 μL，考察不同浓度乙醇（0%、20%、40%、60%、80%、95%）提取的丹参中 12 种成分的含量。结果 在