人参皂甙Rg3在体外对胃癌细胞生长和凋亡的影响

人参皂甙Rg3在体外对胃癌细胞生长和凋亡的影响
王吉;史桂英;袁耀宗;乔敏敏;章永平;孙颖;胡梅洁
【期刊名称】《上海交通大学学报（医学版）》
【年(卷),期】2009(029)011
【摘要】Objective To investigate the effects of ginsenoside Rg3 on growth and apoptosis of gastric cancer cell line MKN-45 and SGC-7901 in vitro. Methods MKN-45 and SGC-7901 cells at logarithmic growth phase were obtained, and were cultured with ginsenoside Rg3 of different concentrations (20, 30, 40, 50 μg/mL) for 24, 48 h or 24, 48 and 72 h. Cells cultured without ginsenoside Rg3 were served as controls. The inhibition rates of ginsenoside Rg3 on MKN-45 and SGC-7901 cells were detected by MTT assay, apoptosis rate of SGC-7901 cells was determined by Annexin V/PI double staining flow cytometry, cell cycles of SGC-7901 cells were analysed by flow cytometry, and morphological changes of SGC-7901 cells in 50 μg/mL ginsenoside Rg3 treatment group were observed by transmission electron microscopy. Results The inhibition rates on MKN-45 and SGC-7901 cells in each ginsenoside Rg3 treatment group were significantly higher than those in control group (P < 0.05), and the inhibition rates increased with the concentrations of ginsenoside Rg3 and time of culture ( P < 0.05). Compared with control group, the apoptosis rates of SGC-7901 cells and percentages of cells in G_1/G_1 cell cycle in each ginsenoside Rg3 treatment group were significantly increased in a
concentration and time dependent manner. Typical morphology of SGC-7901 cell apoptosis was observed by transmission electron microscopy in 50 μg/mL ginsenoside Rg3 treatment gro up. Conclusion Ginsenoside Rg3 has significant inhibition effect on gastric cancer cell lines in vitro with a concentration and time dependent manner, the mechanism of which may involve the induction of gastric cell line apoptosis.%目的研究人参皂甙Rg3在体外对低、中分化胃腺癌细胞株MKN-45和SGC-7901生长及凋亡的影响.方法取处于对数生长期的MKN-45和SGC-7901细胞,加入不同终浓度(20、30、40、50 μg/mL)的人参皂甙Rg3分别培养24、48 h或24、48和72 h,以不加药细胞作为阴性对照.MTT法检测MKN-45和SGC-7901细胞生长抑制率;流式细胞仪Annexin V/PI双染法检测SGC-7901细胞凋亡率;流式细胞仪分析SGC-7901细胞周期;透射电子显微镜观察50 μg/mL人参皂甙Rg3处理组SGC-7901细胞的形态学改变.结果人参皂甙Rg3各处理组SGC-7901和MKN-45细胞生长抑制率均明显高于对照组(P<0.05),且随药物浓度的增加和作用时间的延长而上升
(P<0.05).与对照组比较,人参皂甙Rg3各处理组SGC-7901细胞凋亡率和
G_0/G_1期细胞百分比均明显上升,且呈浓度和时间依赖性.透射电子显微镜观察50 μg/mL人参皂甙Rg3处理组SGC-7901细胞,可见典型的凋亡细胞结构.结论人参皂甙Rg3在体外对胃癌细胞生长具有抑制作用,且呈现一定的时效和量效关系,其机制可能与诱导胃癌细胞凋亡有关.
【总页数】5页(P1336-1340)
【作者】王吉;史桂英;袁耀宗;乔敏敏;章永平;孙颖;胡梅洁
【作者单位】医学院瑞金医院卢湾分院消化内科,上海,200025;基础医学院细胞生物学教研室;医学院瑞金医院消化内科,上海,200025;医学院瑞金医院消化内科,上
海,200025;医学院瑞金医院消化内科,上海,200025;医学院瑞金医院卢湾分院消化内科,上海,200025;医学院瑞金医院消化内科,上海,200025
【正文语种】中文
【中图分类】R735.2
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