两种诱导骨髓间充质干细胞向GABA能神经元分化的比较

两种诱导骨髓间充质干细胞向GABA能神经元分化的比较钟俊;薛卫星;刘卫平;龙乾发;王彦刚;伊西才;刘远杨;杨扬;贾晨光;魏礼洲
【期刊名称】《中华神经外科疾病研究杂志》
【年(卷),期】2012(11)2
【摘要】Objective To compare two methods of inducing rat bone marrow mesenchymal stem cells (BMSCs) to differentiate into γ-aminobutyric acid (GABA) neurons in vitro. Methods BMSCs isolated from SD rats femur bone were cultured and identified. Induction was performed by two methods. BMSCs in control group were cultured with B27, basic libroblast growth factor ( bFGF) , epidermal growth factor (EGF), brain-derived neurotrophic factor (BDNF), all transretinoic acid (ATRA) and 10% fetal bovine serum (FBS) +Dulbecco's modified eagle's medium/nutrient mixture F12
( DMEM/F12); BMSCs in the experimental group were cultured with B27, ATRA, N5, O2'dibutyryladenosine 3':5'-cyclic phosphate cyclic adenosine monophosphate (Bt2CAMP) and 10% FBS + DMEM/F12. The differentiated BMSCs were identified by morphological observation and immunofluorescence staining. Results Neuronal morphological changes were observed in both control group and experimental group. However, the morphological changes in experimental group were much more apparent. BMSCs in two groups showed positive reaction to nestin and GAD-67, the GABA neurons marker. GAD-67 positive rates in control group and experimental group were 33.13% ±5. 12% and 41.06% ±6. 32%,
respectively. There was significant difference between two groups (P<0.05). Conclusion The induction method in experimental group is more efficient
in inducing BMSCs to differentiate into GABA neurons in vitro.%目的比较两种体外诱导大鼠骨髓间充质干细胞(BMSCs)向γ-氨基丁酸(GABA)能神经元分化的效果.方法从SD大鼠股骨骨髓中分离出BMSCs,进行培养、鉴定.用两种不同的方
法进行诱导,方法一设为对照组:碱性成纤维细胞生长因子(bFGF)、表皮生长因子(EGF)、脑源性神经营养因子(BDNF)、全反式维甲酸(ATRA)诱导；方法二设为实
验组:ATRA、N5,O2'-二丁酰腺苷3'∶5'-环磷酸(Bt2环磷腺苷)(Bt2CAMP)诱导.对分化细胞进行形态学观察和免疫荧光染色.结果两组细胞均出现类似神经细胞的形态变化,实验组细胞变化更明显.两组细胞均能表达nestin和GABA 能神经元标记GAD-67,对照组与实验组表达GAD-67的阳性率分别为33.13％±5.12％和41.06％±6.32％,两组相比较具有统计学意义(P＜0.05).结论实验组定向分化为GABA能
神经元的效率高于对照组.
【总页数】4页(P141-144)
【作者】钟俊;薛卫星;刘卫平;龙乾发;王彦刚;伊西才;刘远杨;杨扬;贾晨光;魏礼洲【作者单位】第四军医大学西京医院神经外科,陕西西安710032;第四军医大学西
京医院神经外科,陕西西安710032;第四军医大学西京医院神经外科,陕西西安710032;第四军医大学西京医院神经外科,陕西西安710032;西安市中心医院神经
外科,陕西西安710001;第四军医大学西京医院神经外科,陕西西安710032;第四军
医大学西京医院神经外科,陕西西安710032;第四军医大学西京医院神经外科,陕西
西安710032;第四军医大学西京医院神经外科,陕西西安710032;第四军医大学西
京医院神经外科,陕西西安710032;第四军医大学西京医院神经外科,陕西西安710032
【正文语种】中文
【中图分类】R329.2
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