SBC-115076_489415-96-5_DataSheet_MedChemExpress

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捷尼科比亚 慢病毒表达包装试剂盒 使用手册说明书

捷尼科比亚 慢病毒表达包装试剂盒 使用手册说明书

Lenti-Pac ™ HIV Expression Packaging KitFor optimized production of recombinant lentivirusCat. No. HPK-LvTR-20 (20 transfections) Cat. No. HPK-LvTR-40 (40 transfections)User Manual Version VGeneCopoeia, Inc.9620 Medical Center Drive, #101 Rockville, MD 20850 USA301-762-0888 or 866-360-9531***********************© 2009 GeneCopoeia, Inc.G C n p o eiae e o TMExpressway to DiscoveryUSER MANUALLenti-Pac™ HIV Expression Packaging KitI. IntroductionII. Kit Contents and StorageIII. Additional Materials Required or RecommendedIV. Getting StartedV. Lentivirus ProductionVI. Lentivirus Titer Estimation by TransductionVII. Transduction of Target Cells with LentivirusesVIII. Limited Use License and WarrantyI. IntroductionGeneCopoeia has multiple sets of over 40,000 human and mouse ORF expression clones as well as multiple sets of small hairpin RNAi (shRNA) clones against genome-wide target genes from human, mouse, rat, and other mammals in HIV-based lentiviral vector systems. HIV- (human immunodeficiency virus) based vectors are currently the most popular lentiviral-based expression systems and are very effective at transducing genes into a wide variety of dividing and non-dividing mammalian cells, both in vitro and in vivo. The lentiviral expression vectors can integrate into the genome of the target cells, resulting in the stable expression of transgenes. The GeneCopoeia third generation HIV-based lentivector systems meet Biosafety Level 2 (BSL-2) requirements based on the criteria published by the Centers for Disease Control.The GeneCopoeia Lenti-Pac™ HIV Expression Packaging System includes an optimized lentiviral packaging plasmid mix, an eGFP positive control plasmid, a new transfection reagent, EndoFectin™optimized for virus production and TiterBoost™ reagent that further increases the titers 5-10 fold. When combined with GeneCopoeia HIV-based lentiviral constructs the results are high titers and robust expression levels. The Lenti-Pac HIV Expression Packaging System safely ensures efficient expression of recombinant transcripts in mammalian cells.A dvantages of OmicsLink™ Lentiviral ORF Expression Clones and shRNA Clones∙High efficiency of gene delivery to virtually all mammalian cell types in vitro as well in vivo∙High expression levels of delivered genes (ORF expression clones)∙High knockdown efficiency against target mRNA transcripts (shRNA clones)∙Self-inactivation and no unwanted viral replicationII. Contents and Shipping/ StorageContents and storage recommendations for the Lenti-Pac HIV Expression Packaging Kits(Cat. No. HPK-LvTR-20/HPK-LvTR-40) are provided in the following table.III. Additional Materials Required or Recommended1. GeneCopoeia GCI-L3 chemically competent cells (GeneCopoeia Cat No. STK300-10). Alternatively, Stbl3™chemically competent cells (Invitrogen Cat No. C7373-03) may be used.2. GeneCopoeia 293Ta Lentiviral packaging cell line(GeneCopoeia Cat No. Clv-PK-01). Alternatively, theHEK 293T/17 cell line (ATCC Cat No. CRL-11268) can also be used.3. H1299 cell line (ATCC Cat No.CRL-5803), or HT-1080 cell line (ATCC Cat No. CCL-121) for lentivirus titerestimation. H1299 cells are preferred over HT-1080 cells for lentivirus titration.4. DMEM with glucose, L-glutamine and sodium pyruvate (Mediatech Cat No. 10-013-CV)5. Fetal bovine serum (Thermo Scientific Cat No. SH300700.02)6. Opti-MEM® I Reduced-Serum Medium (Invitrogen Cat No. 31985-062/31985-070).7. Polybrene(Sigma-Aldrich Cat No. H9268): 10 mg/ml solution dissolved in 150 mM NaCl and sterile-filtered.Store usable aliquots at -20o C. Working stock can be stored at 4o C for up to two months.8. Crystal Violet (Sigma-Aldrich Cat No. C3886): 0.5% (W/V) solution dissolved in 25% methanol.9. Penicillin-Streptomycin for mammalian cell culture (Sigma-Aldrich Cat No. P4333)10. Antibiotics for selecting stably transduced cells: puromycin (Invivogen Cat No. ant-pr-1/ant-pr-5), hygromycinB (Invivogen Cat No. ant-hm-1/ant-hm-5), Neomycin (G-418) (Invivogen Cat No. ant-gn-1/ant-gn-5)11. BD Falcon® 5-ml or 14-ml Tubes (BD Falcon Cat No. 352053/352059).IV. Getting StartedUpon receipt of a new lentiviral expression plasmid, it is recommended to transform the plasmid into GeneCopoeia GCI-L3 Chemically Competent E. coli Cells (GeneCopoeia Cat No. STK300-10), or any Stbl3™-equivalent competent cell strain, and to prepare plasmid DNA with a proven plasmid DNA purification method. GeneCopoeia lentiviral ORF expression or shRNA constructs contain the ampicillin resistance gene.Quality of plasmidIt is critical to use plasmid of the highest quality. Determine the DNA concentration by reading the absorption at 260 nm. DNA purity is measured by using the 260 nm / 280 nm ratio (the ratio should be in the range of 1.8 to 2.0). Check the integrity of the plasmid by agarose gel electrophoresis.Condition of cellsAlways use healthy cells that are well maintained and passaged regularly. Make sure the culture is free from bacteria, fungi, or Mycoplasma contamination. If the cells are from a recent liquid nitrogen stock, passage the cells at least 2 times before transfection.V. Lentivirus ProductionThe GeneCopoeia HIV-Based Lentiviral Expression System is a modified version of the third generation self-inactivating (SIN) lentiviral vector system which incorporates enhanced bio-safety features and is optimized for production of high viral titers. In this system, recombinant lentiviral particles are generated by co-transfecting an HIV-based lentiviral expression plasmid together with the GeneCopoeia Lenti-Pac HIV Expression Packaging Kit into GeneCopoeia 293Ta lentiviral packaging cells (GeneCopoeia Cat No. CLv-PK-01).The lentiviral ORF/shRNA expression plasmid (lentiviral transfer vector) contains the elements required for packaging, transduction and stable integration of the viral expression construct into genomic DNA leading to expression of the ORF or shRNA hairpin. However, it lacks the elements essential for transcription and packaging of an RNA copy of the ORF/shRNA expression plasmid into recombinant pseudoviral particles. These elements are provided by the Lenti-Pac HIV packaging mix, an optimized mixture of plasmids that express the structural, regulatory, and replication genes required to produce lentivirus. See figure 1 below for schematics of this process. The lentivirus generated with this system is pseudotyped with vesicular stomatitis virus-G protein (VSV-G) which exhibits wide cell tropism and generates high titers.In addition to Lenti-Pac HIV packaging mix, this kit also includes a positive control lentiviral transfer vector that expresses the eGFP protein, EndoFectin Lenti Reagent (Cat No. EFL1001-01), and TiterBoost reagent. The EndoFectin Lenti transfection reagent is optimized for transferring plasmids into packaging cells. It guarantees higher transfection efficiency and lower cell toxicity compared to other commercially available transfection reagents. The unique TiterBoost reagent from GeneCopoeia enhances the production of lentivirus particles.Figure 1. Schematics of Lentivirus production and infection of target cellsThe following procedure provides optimized steps for lentivirus production in 293Ta packaging cells. The yield of recombinant lentiviral particles typically produced under these optimized conditions is 10 ml of 1–10 x107 infection units (ifu) per ml of un-concentrated supernatant from one 10-cm culture dish for eGFP or mCherry positive controls. This amount of pseudoviral particles is generally sufficient to infect 1–10 x108 target cells at a MOI (multiplicity of infection) equal to 1. The titers of lentivirus decrease as the size of insert increases. Actual lentivirus titers for your gene of interest will vary accordingly.Caution: Following this protocol results in the production of pseudoviral particles capable of infecting mammalian cells. The recommended guidelines for working with BSL-2 safety class must be adhered to.1. Plate packaging cellsTwo days before transfection, plate 1.3–1.5 x106of the GeneCopoeia 293Ta lentiviral packaging cells or comparable cells in a 10-cm dish in 10 ml of DMEM supplemented with 10% heat-inactivated fetal bovine serum so that the cells are 70–80% confluent at the moment of transfection. Incubate the cells at 37°C with 5% CO2.Note: Plating the packaging cells 2 days prior to transfection significantly increases the titer of lentivirus.Use heat-inactivated fetal bovine serum for lentivirus production. Heat-inactivated serum can be purchased from other vendors or prepared by incubating thawed serum for 30 minutes at 56°C with gentle shaking.2. Prepare DNA/EndoFectin Lenti complexIn a sterile polypropylene tube, dilute 2.5 µg of lentiviral ORF/shRNA expression plasmid and 5.0 µl (0.5 µg/µl) of Lenti-Pac HIV mix into 200 µl of Opti-MEM® I (Invitrogen). In a separate tube, dilute 15 µl of EndoFectin Lenti into 200 µl of Opti-MEM I. Add diluted EndoFectin Lenti reagent drop-wise to the DNA solution while gently vortexing the DNA-containing tube. Do not reverse the addition sequence. Use round-bottom polypropylene tubes such as Falcon® 5-ml or 14-ml tubes (BD) for larger volumes. Incubate the mixture for 10–25 minutes atroom temperature to allow the DNA-EndoFectin complex to form.Note: The DNA-EndoFectin complex must be formed in the absence of proteins even though the complex is able to transfect cells in the presence of proteins such as 10% serum. Opti-MEM I is recommended for diluting both DNA and EndoFectin Lenti reagent. Serum-free DMEM can be used in place of Opti-MEM I but the transfection efficiency will be compromised. The ratio of 3.0 µl of EndoFectin Lenti per 1 µg of plasmid has been found to be optimal. Increasing the ratio does not further improve transfection efficiency.3. Transfect packaging cellsAdd the DNA-EndoFectin Lenti complex directly to each dish and gently swirl the dish to distribute the complex.Incubate the cells in a CO2 incubator at 37°C overnight (8–14 hours). Replace the overnight culture medium with fresh DMEM medium supplemented with 2–5% heat-inactivated fetal bovine serum and penicillin-streptomycin. Add 1/500 volume of the TiterBoost reagent to the culture medium and continue incubation in the CO2 incubator at 37°C.Note:a. Replace the culture medium that contains the DNA-EndoFectin Lenti complex within 16 hours post-transfection.b. TiterBoost reagent at working concentration (1×) typically boosts the titer of lentivirus products 5-10 fold.This reagent is readily removed during commonly used lentivirus concentration/purification procedures such as ultracentrifugation, ultrafiltration and other chromatographic methods. If crude lentiviral particles are used directly to transduce target cells, the volume of lentiviral particles should not exceed 1/10 of that of culture medium; otherwise some adverse effects may occur. TiterBoost at 1/20× or lower concentrations has negligible effects on commonly used mammalian cell lines. It's advised to test the effects of TiterBoost on your target cells beforehand if large volumes of crude lentiviral particles are used.4. Harvest lentivirusCollect the pseudovirus-containing culture medium in sterile capped tubes 48 hours post transfection and centrifuge the tubes at 500 x g for 10 minutes to get rid of cell debris. Following centrifugation, filter the supernatant through 0.45 µm polyethersulfone (PES) low protein-binding filters.Note:a. Peak virus production is normally achieved 24–48 hours post transfection. Alternatively, lentivirus-containing medium may be collected multiple times at 36, 48 and 60 hours post-transfection. Lentiviral containing supernatants should be replaced with fresh DMEM supplemented with 2–5% heat-inactivated fetal bovine serum, penicillin-streptomycin and TiterBoost reagent.b. Do not use nitrocellulose filters as nitrocellulose is known to bind lentivirus and reduce titers.The supernatant containing lentiviral particles can be used directly to determine the titer and to transduce target cells in vitro as long as the target cells can survive in conditioned medium. Lentiviral stocks should be aliquoted and stored at -80°C. Expect significant loss of viral titer with each freeze/thaw cycle.VI. Lentivirus Titer Estimation by TransductionAt this point it is recommended that the pseudoviral stock is titered to ensure it is viable and to test what fraction of target cells can be transduced. This enables the number of copies of viral construct per target cell to be controlled. There are several methods to determine the titer of pseudovirus stock. The procedure below is based on the transduction of H1299 or HT-1080 cells. Different cells can also be used but titers may vary up to several orders of magnitude.Day 1: Plate H1299 or HT-1080 cells1. Plate ~5 x 104 of the H1299 or HT-1080 cells per well in a 24-well plate 24 hours prior to viral infection. Use 0.5 ml of DMEM supplemented with 10% heat-inactivated fetal bovine serum and penicillin-streptomycin for each well. Incubate the cells at 37°C with 5% CO2 overnight.Day 2: Transduce H1299 or HT-1080 cells2. Dilute Polybrene to 10 µg/ml with DMEM containing 5% heat-inactivated fetal bovine serum and penicillin-streptomycin.3. Remove old culture medium from each well. Add 0.25 ml of Polybrene (from Step 2). The final concentration of Polybrene will be 5 µg/ml after adding diluted lentivirus. For each pseudoviral stock, use five wells.3a. For lentivirus containing a fluorescent marker and to be analyzed with flow cytometry (step 5a): Infect H1299 or HT-1080 cells by adding 0.1 μl of lentivirus (10 μl of 100-fold diluted viral stock) into the first well, 0.5 μl of lentivirus (50 μl of 100-fold diluted viral stock) into the second well, 2.0 μl into the third well, 10 μl into the fourth well, and 50 μl into the fifth well. Add appropriate amount of DMEM containing 5% heat-inactivated serum and penicillin-streptomycin so that the final volume reaches 0.5 ml per well.3b. For lentivirus to be analyzed by drug selection and colony counting (step 5b-10): Infect H1299 or HT-1080 cells by adding 0.001 μl of lentivirus (10 μl of 10,000-fold diluted viral stock) into the first well, 0.01 μl of lentivirus (10 μl of 1,000-fold diluted viral stock) into the second well, 0.1 μl of lentivirus (10 μl of 100-fold diluted viral stock) into the third well, 0.5 μl of lentivirus (50 μl of 100-fold diluted viral stock) into the fourth well, and 2.0 μl into the fifth well. Add appropriate amount of DMEM containing 5% heat-inactivated serum and penicillin-streptomycin so that the final volume reaches 0.5 ml per well.Place the plates for 2 hours at 4-8°C; then transfer the plates to a 37°C incubator with 5% CO2 and incubate cells overnight.Note:a. Dilute lentivirus with only culture medium. Do not use water or other buffers.b. The optimal concentration of Polybrene depends on cell type and may need to be empirically determined,but is usually in the range of 2–10 µg/ml. Prepare enough for an extra well as a negative control.c. Excessive exposure to Polybrene (>12 hr) can be toxic to some cells.Day 3: Replace medium/split cell culture4. Trysinize and transfer the cells to 6-well plates (each 6-well plate will be used for one lentivirus with one control well of non-transduced cells). Incubate in DMEM supplemented with 10% fetal bovine serum and penicillin-streptomycin for additional 48 hours.Day 5: Determine titer by fluorescence analysis (step 5a) or drug selection (5b)5a. The fraction of eGFP fluorescent cells can be counted by FACS (fluorescent activated cell sorting). Alternatively the eGFP fluorescence may be visualized under a fluorescent microscope. Normally 10 random fields of view are used to estimate the overall fraction of fluorescing cells in each well. The cells are then trypsinized, suspended with complete DMEM, and the total number of cells in each well is determined by using a hemocytometer. The averaged fraction of fluorescent cells is multiplied by the corresponding total cell numbers, then divided by the actual volume of added lentivirus supernatant (in ml, e.g. 0.1 μl equals 10–4 ml) to determine the titer of the pseudovirus in the supernatant.5b. For HT-1080 or H1299 cells transduced with lentiviral stocks lacking a fluorescent marker, replace the old medium with fresh complete DMEM containing an appropriate selection drug. (Note: The concentration of the selection drug should be determined empirically beforehand.) Then follow steps 6–10 below:Days 6-14: Select stably transduced cells and count colonies (continued from step 5b)6. Replace medium with fresh complete medium containing the appropriate selection drug every 3–4 days until drug-resistant colonies become visible (generally 7–14 days after selection). There should not be any colonies in the mock well control.7. Remove medium and wash dishes twice with cold PBS.8. Add enough 10% formalin to cover each dish. Incubate for 5 minutes at room temperature to fix the cells.9. Decant 10% formalin, add 0.5% crystal violet and incubate for 10 minutes at room temperature.10. Remove the crystal violet solution and wash the dishes with tap water until there is no background staining. Count the blue colonies and calculate the titer of lentiviral stock.Note:Viral titers determined by drug selection/colony counting are significantly lower than those determined by FACS.VII. Transduction of Target Cells with LentivirusesThe transduction efficiency depends upon the target cells and experimental procedure. It is recommended that the titrated pseudoviral stock containing the positive control eGFP is used to determine the concentration of pseudoviral particles required for the desired MOI of the target cells. After these test transductions are performed, it should be possible to determine the optimum concentration of pseudoviral particles for transduction based on eGFP fluorescence.Day 1: Plate cells1. Plate 2–10 x 104 of the target cells per well in a 24-well plate 24 hours prior to viral infection. Use 0.5 ml of DMEM supplemented with 5% heat-inactivated serum and penicillin-streptomycin for each well. Incubate the cells at 37°C with 5% CO2 overnight.Day 2: Transduce target cells2. For each well, prepare 0.5 ml of virus suspension diluted in complete medium with Polybrene at a final concentration of 5–8 µg/ml.Note:Use several dilutions of pseudoviral stock (0.1 μl to 100 μl). In addition, we recommend including a transduction with the eGFP control and other appropriate positive and negative controls. Mix the virus with the medium gently by rotation or inversion. Do not vortex.3. Infect the target cells by removing the old culture medium and replacing it with 0.5 ml of diluted viral supernatant. For one well (mock well control), add 0.5 ml of complete DMEM with Polybrene. Place the plates in a 37°C incubator with 5% CO2 and incubate cells overnight. (Optional: Place the plates for 2 hours at 4-8°C; then transfer the plates to a 37°C incubator with 5% CO2 and incubate cells overnight.)Note:Incubating cells with lentivirus for 2 hours at low temperatures can significantly increase the transduction efficiency. But it may be omitted if the cells cannot tolerate low temperatures.Day 3: Replace medium/Split cell culture4. Remove the culture medium and replace with 0.5 ml of complete medium (without Polybrene). Or split the cells 1:3 to 1:5 depending on the type of cells, and continue incubating for 48 hours in DMEM.Day 5: Analyze transduced cells or start drug selection of stably transduced cells5a. The infected target cells can be analyzed for transient expression of transgenes using an appropriate biological assay. If you have used an internal eGFP control, determine the percentage of infected cells by counting fluorescing cells by flow cytometry or with a fluorescent microscope.5b. To select stably transduced cells, replace old medium with fresh complete medium containing the appropriate selection drug every 3–4 days until drug-resistant colonies become visible (generally 7–14 days after selection).VIII. Limited Use License and WarrantyLimited Use LicenseFollow ing terms and conditions apply to use of all OmicsLink™ ORF Expression Clones in all lentiviral vectors and Packaging Kit (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms.Limited WarrantyGeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoe ia’s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia’s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose.GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at (301)-762-0888.© 2009, GeneCopoeia, Inc.GeneCopoeia, Inc.9620 Medical Center Drive, #101Rockville, Maryland 20850Tel: 301-762-0888 Fax: 301-762-8333Email:***********************Web: GeneCopoeia Products are for Research Use Only Copyright © 2009 GeneCopoeia, Inc.。

KSZ8851-16MLL DEMO BOARD 48-PIN ETHERNET CONTROLLE

KSZ8851-16MLL DEMO BOARD 48-PIN ETHERNET CONTROLLE

SD13
SD7 40
CPU_D14 3
6
SD14
SD8 39
CPU_D15 4
5
SD15
SD9 36
SD10 35
33
SD11 34
CPU_CMD
33
SD12 33
R10
SD13 32
CPU_CSN
33
SD14 31
R12
SD15 30
CPU_WRN
33
CMD
11
R14
CPU_RDN
33
CSN
12
R16
5 6 7 8
TANT
C27
R28 10uF
470pF 2.49K
Power 3.3V 0.1uF (red LED)
CSN CMD
4.7K R27 4.7K R29
GBLC03C_0 D3
GND 2 GND
VR 5 3.3VA
INTRN 4.7K R30
VOUT = 1.24 X [ 1 + ( 2.49k/ 1.5K ) ]
5
4
3
KSZ8851-16MLL (48-pin) Demo Board Black Diagram
D
Headers 20x2
RJ45
LAN1 T
KSZ8851-16MLL
Reset
Power
+1.8V
+2.5V
+3.3V
STATUS LEDs
OSC
EEPROM
C
MIC5209YM
25 MHz
AT93C46
x2
2
1
DATE:

超米特电子有限公司产品说明书

超米特电子有限公司产品说明书

1US Headquarters TEL +(1) 781-935-4850FAX +(1) 781-933-4318 • Europe TEL +(44) 1628 404000FAX +(44) 1628 404090Asia Pacific TEL +(852) 2 428 8008FAX +(852) 2 423 8253South America TEL +(55) 11 3917 1099FAX +(55) 11 3917 0817Superior elongation and tensilestrength help to prevent tearing in use due to mishandling. Typical properties for CHO-SEAL 1310 and 1273 materi-al are shown on pages 33 and 32respectively.High Shielding PerformanceCHO-SEAL 1310 material provides more than 80 dB of shielding effectiv-ness from 100 MHz to 10 GHz, while CHO-SEAL 1273 material provides more than 100 dB.Low Volume ResistivityBoth materials have exceptionally low volume resistivity, which makes them well suited for grounding appli-cations in which a flexible electrical contact is needed.Low Compression GasketSpacer gaskets are typicallydesigned to function under low deflec-tion forces. Chomerics uses design tools such as Finite Element Analysis (FEA) to accurately predict compres-sion-deflection behavior of various cross section options. Refer to page16.LCP Plastic SpacerLiquid crystal polymer (LCP)spacers, including those made with Vectra A130 material, provide aCHO-SEAL ®1310 or 1273Conductive ElastomersWith EMI spacer gaskets, shielding and grounding are provided by Chomerics’CHO-SEAL 1310 and 1273 conductive elastomers, specifi-cally formulated for custom shape molded parts. They provide excellent shielding and isolation against electro-magnetic interference (EMI), or act as a low impedance ground path between PCB traces and shielding media. Physically tough, these elas-tomers minimize the risk of gasket damage, in contrast to thin-walled extrusions or unsupported molded gaskets.Silicone-based CHO-SEAL 1310and 1273 materials offer excellent resistance to compression set over a wide temperature range, resulting in years of continuous service. CHO-SEAL 1310 material is filled with silver-plated-glass particles, while 1273 utilizes silver-plated-copper filler to provide higher levels of EMI shielding effectiveness.EMI Spacer GasketsThe unique design of Chomerics’EMI spacer gaskets features a thin plastic retainer frame onto which a conductive elastomer is molded. The elastomer can be located inside or outside the retainer frame, as well as on its top and bottom surface. EMI spacer gaskets provide a newapproach to designing EMI gaskets into handheld electronics such as dig-ital cellular phones. Board-to-board spacing is custom designed to fit broad application needs. Customized cross sections and spacer shapes allow for very low closure forcerequirements and a perfect fit in any design or device.Robotic InstallationSpacer gaskets can be installed quickly by robotic application. Integral locater pins in the plastic spacer help ensure accuratepositioning in both manual and pick-and-place assembly. Benefits include faster assembly and lower labor costs.The integrated conductive elastomer/plastic spacer gasket is a low cost,easily installed system for providing EMI shielding and grounding in small electronic enclosures.Figure 1Single Piece EMI Gasket/Locator PinsCHO-SEAL 1310 or 1273 Conductive Elastomer (Inside)Plastic Spacer Around Outsideor InsideApplications for EMI Spacer GasketsThe spacer gasket concept is especially suited to digital and dual board telephone handsets or other handheld electronic devices. It provides a low impedance path between peripheral ground traces on printed circuit boards and components such as:•the conductive coating on a plastic housing•another printed circuit board •the keypad assemblyTypical applications for EMI spacer gaskets include:•Digital cellular, handyphone and personal communications services (PCS) handsets •PCMCIA cards•Global Positioning Systems (GPS)•Radio receivers•Other handheld electronics, e.g.,personal digital assistants (PDAs)•Replacements for metal EMI shield-ing “fences” on printedcircuit boards in wireless tele-communications devicesstable platform for direct, highprecision molding of conductive elas-tomers. The Vectra A130 material described in Table 1 has excellent heat deflection temperature character-istics (489°F, 254°C). For weight con-siderations, the LCP has aspecific gravity of only 1.61. This plas-tic is also 100% recyclable.Typical EMI Spacer Gasket Design ParametersThe EMI spacer gasket concept can be considered using the design parameters shown in Table 2. Some typical spacer gasket profiles are shown below.Figure 2Typical Spacer Gasket Profiles3US Headquarters TEL +(1) 781-935-4850FAX +(1) 781-933-4318 • Europe TEL +(44) 1628 404000FAX +(44) 1628 404090Asia Pacific TEL +(852) 2 428 8008FAX +(852) 2 423 8253South America TEL +(55) 11 3917 1099FAX +(55) 11 3917 0817Finite Element AnalysisChomerics, a division of the Parker Hannifin Corporation’s Seal Group, is the headquarters of Parker Seal’s Elastomer Simulation Group. This unit specializes in elastomer finite element analysis (FEA) using MARC K6 series software as a foundation for FEA capability.Benefits of FEA include:•Quickly optimizing elastomer gasket designs•Allowing accurate predictions of alternate elastomer design concepts •Eliminating extensive trial and error prototype evaluationTypical use of FEA in EMI spacer gasket designs is to evaluate the force vs. deflection requirements of alternate designs.For example, onespacer design features a continuous bead of con-ductive elastomer molded onto a plastic spacer. An alternative designemploys an “interrupted bead,” where the interrup-tions (gaps left on the plastic frame) are sized to maintain the requiredlevel of EMI shielding. Figure 4illustrates these alternative designs.Gasket DeflectionFigure 5 compares the effect of continuous and interrupted elastomer gasket designs in terms of the force required to deflect the conductive elastomer. This actual cellular handset application required a spacer gasket with interrupted bead to meet desired deflection forces.Chomerics Designand Application ServicesChomerics will custom design a spacer for your application. Advice,analysis and design assistance will be provided by Chomerics Applications and Design engineers at no additional fee. Contact Chomerics directlyat the locations listed at the bottom of the page.Figure 3FEA Example of an EMISpacer Gasket Cross SectionFigure 4Continuous (top) and InterruptedElastomer GasketsFigure 5Typical Spacer Gasket Deflection。

Fujitsu STYLISTIC Q7311 商品说明书

Fujitsu STYLISTIC Q7311 商品说明书

Data SheetFujitsu STYLISTIC Q7311Tablet Mobility Meets Notebook ProductivityThe FUJITSU Tablet STYLISTIC Q7311 is a highly durable and secure 2-in-1 detachable that meets the demands of today’s mobile professionals. Its 33.8 cm (13.3-inch) FHD anti-glare display comes with pen and touch support. The latest 11th generation Intel® Core™ i7 vPro® processor technology and Intel® Thunderbolt™ 4 ensure excellent performance, even when you’re on the go.Combined tablet mobility and notebook productivity in one device - whereever you areThe robust IP42 compliant housing with reinforced corners and port caps protects your device from damages, even outside the office.Highly durable IP42 compliant housing with reinforced cornersPort caps that protect the interfaces like the new Intel® Thunderbolt™ 4 from water spills Optional backlit keyboard dock with LAN and charging port Optional TPU cover for more robustness Always connected and instantly readyConvenience at workSign documents legally secure. The pen is automatically charged within seconds when stored in its pen garage, that protects it also from being lost. Ready whenever you need it. 33.8 cm (13.3-inch) Full HD anti-glare touch display Supporting pen with 4K pressure levelsPen garage with inductive charging functionLean, agile and secureMore freedom to work safely from anywhere at anytime without compromises in security and data protection – integrated IR Cam with Windows Hello support, fingerprint sensor and Smartcard reader protect your device like a mansion.Weight starting at 800 grams, the device offers you ultimate portability Fingerprint sensor & IR Camera supporting Windows Hello Smartcard readerKensington lock supportUp to 10 hours battery runtimeIntel® Iris® Xe GraphicsComponentsBase unit STYLISTIC Q7311Operating systemsOperating system pre-installed Windows 11 Pro. Fujitsu recommends Windows 11 Pro for business.Windows 11 HomeWindows 10 Pro. Fujitsu recommends Windows 11 Pro for business.Operating system compatible FREE Upgrade to Windows 11**Upgrade timing may vary by device. Features and app availability may vary by region. Certain features requirespecific hardware (see aka.ms/windows11-spec).Operating system notes Internet connectivityWindows 10 Support: After the end of the product life Fujitsu will continue to test and support all upcoming Window10 releases for a period of maximum 5 years – depending on the available extension of hardware services throughFujitsu Warranty top ups. For details please see “Fujitsu Service Statement for Windows 10 Semi-Annual-ChannelSupport” at .Processor Intel® Core™ i7-1185G7 processor (4 Cores / 8 Threads)Intel® Core™ i5-1145G7 processor (4 Cores / 8 Threads)Intel® Core™ i5-1135G7 processor (4 Cores / 8 Threads) ** Processor only for retail, SMB, education and governmentHard disk drives (internal)SSD PCIe, SSD 512GB Value PCIe G3 M.2 FDE, SEDSSD PCIe, SSD 512GB MS PCIe G3 M.2 FDE, SEDSSD PCIe, SSD 256GB Value PCIe G3 M.2 FDE, SEDSSD PCIe, SSD 1TB Value PCIe G3 M.2 FDE, SEDSSD PCIe, SSD 1TB MS PCIe G3 M.2 FDE, SEDHard disk notes One Gigabyte equals one billion bytes, when referring to hard disk drive capacity.Accessible capacity may vary, also depending on used software.Up to 20 GB of HDD space is reserved for system recoveryInterface add on cards/components(optional)4G/ LTE (optional)(Downlink speed up to 300 Mbit/s, Uplink speed up to 150 Mbit/s)Display33.8 cm (13.3-inch), IPS, FHD, 1,920 x 1,080 pixel, Anti-glare touchscreen, 400 cd/m², 1500:1MultimediaCamera Front: HD Cam (0.9MP) with LEDFront: IR Cam (0.9MP) supporting Windows Hello with LEDRear: 5M with LEDBase unitBase unit STYLISTIC Q7311General system informationChipset Integrated in CPUSupported capacity RAM (min.)8 GBSupported capacity RAM (max.)16 GBMemory notes8 GB onboard or 16 GB onboardDual channel supportLPDDR4x (4,266 MHz)LAN notes Virtual MAC address. LAN connector via optional keyboard docking, cradle or optional USB to LAN adapter. Integrated WLAN Intel WiFi 6 AX201 - WLAN, BT, SRD cat. 1General system informationBIOS version UEFI Specification 2.7BIOS features InsydeH2O BIOSAudio type On boardAudio codec Realtek ALC255Audio features2x digital array microphones, 2x built-in speakers (stereo)Waterproof / Spillproof IP42Disinfectable noMIL-STD tested Yes, selected MIL-STD-810H tests passed.MIL-STD-810H test results are not a guarantee of future performance under identified test conditions.Accidental damage is not covered under standard international limited warranty.DisplayDiagonal Size33.8 cm (13.3-inch)Display Technology IPSDisplay type Anti-glare touchscreenDisplay Resolution type FHDBrightness - typical400 cd/m²Contrast - typical1500:1Viewing angle (h/v) - typical178°/178°Display notes Wide-view high-bright LED display (for enhanced outdoor viewing)Integrated ambient light sensor for automatic backlight adjustment to the working environment.Toughened glassSensors Ambient Light SensorCompassGyroscopeProximityDigitizer / Touch Technology Wacom Digitizer for pen (AES) input plus capacitive 10 finger multi-touch screenPen AES4k pressure levelPen garageinductive chargingAmbient light sensor IntegratedAmbient light sensor notes While enabled, the ambient light sensor automatically adjusts the display backlightGraphicsBase unit STYLISTIC Q7311TFT resolution (HDMI)up to 4,096 x 2,160 @ 60 HzGraphics brand name Intel® Iris® Xe Graphics (with Dual channel memory)Graphics features 4 Display Support (3 external, 1 internal)HDCP supportDirectX® 12OpenGL® 4.5Graphics notes Shared memory depending on main memory size and operating system3D acceleratorInterfacesDC-in1Audio: line-out / headphone 1 (combo port with Audio line-in)Audio: line-in / microphone 1 (combo port with Audio line-out)Internal microphones2x digital array microphonesUSB 2.0 total1USB 3.2 Gen1 (5 Gbps) total 1 x Type-AUSB 4.0 Gen3 (20 Gbps) total 1 x Type-C Intel® Thunderbolt™ 4 (with Power Delivery functionality)USB Type-C 1 USB 4 Gen3 Thunderbolt™4, Power Delivery (15W)HDMI 1 v1.4Ethernet (RJ-45)-Memory card slots 1 (USH-I) SD 3.0 StandardSD/microSD card: 2GBSDHC/microSDHC card: 32GBSDXC/microSDXC card: 2TBSmartCard slot optionalSIM card slot 1 (Nano-SIM, only for models with integrated 4G/LTE module)Interface Module notes LAN and VGA available via conversion cable.Docking connector for Port Replicator 1 (Cradle)Kensington Lock support1Port Replicator interfaces (optional)USB Type-C PR CradleDC-in 1 (19V/90W required) 1 (19V)Power on switch1---Audio: line-in---1Audio: line-in / line-out1---Audio: line-out---1Audio: comments Combo jack for headset usage---USB 3.2 Gen1 (5 Gbps) total---3DisplayPort1x V1.2 1VGA1x1HDMI text1x------Interface Notes3x Type-A - 5V/0.9A, 4.5W1x Type-C - 15W1x Type-C - Up to 60 W (PD v2.0-1.1) power output toclient or 4.5W inputKensington Lock support no1Ethernet (RJ-45)1 1 (10/100/1000)---Notes Number of simultaneous used displays and its possibleresolutions and frequencies depend on mobile systemand display interface type.Please consult always also the manual of the connectedclient.Wireless technologiesAntennas2x dual band for WLAN, 2x for LTE, Bluetooth shared with WLANBluetooth V5.1Integrated WLAN Intel WiFi 6 AX201 - WLAN, BT, SRD cat. 1WLAN encryption WPA/WPA2/WPA3 (Wi-Fi Protected Access)WLAN notes Import and usage according to country-specific regulations.Integrated WWAN LTE Sierra Wireless EM7421 (Cat.7) - UMTS, LTEWWAN notes Including GPS functionalityImport and usage according to country-specific regulations.LTE Connection Manager (if configured with 4G/LTE)GPS Embedded in 4G module if configured with WWANPower supplyAC adapter19 V / 65 W (3.42 A)20 V / 65 W (3.25 A) Type CNotes65W AC Adapter for usage with system/ min. 90W AC Adapter for usage with Cradle Rated voltage range100 V - 240 V (AC Input)Rated frequency range50 Hz - 60 Hz1st battery Li-Ion battery 3-cell, 38 WhBattery features Quick Charge: 80% in 1hRuntime 1st battery10 hBattery notes Battery runtime information is based on worldwide acknowledged BAPCo® MobileMark® 2018. Refer to www.bapco.com for additional details.The BAPCo® MobileMark® Benchmark provides results that enable direct product comparisons betweenmanufacturers. It does not guarantee any specific battery runtime which actually can be lower and may varydepending on product model, configuration, application and power management settings. The battery capacitydecreases slightly with every re-charge and over its lifetime.Dimensions / Weight / EnvironmentalDimensions (W x D x H)315 x 200.9 x 10.5 mm12.4 x 0.79 x 0.42 inchWeight from 0.800 kg slate only, weight of keyboard docking from 0.465 kgWeight (lbs)from 1.75 lbs slate only, weight of keyboard docking from 1.25 lbsWeight notes Weight may vary depending on actual configurationOperating ambient temperature 5 - 35 °C (41 - 95 °F); Packed -10 - 60 °C (14 - 140 °F)Operating relative humidity Running: 20 - 80% (relative humidity); Packed: 20 - 80% (relative humidity)Product STYLISTIC Q7311Germany GSEurope CECBGlobal TCO Certified 8.0EPEAT® Silver (dedicated regions), depending on configurationMicrosoft Operating Systems (HCT / HCL entry / WHQL)MIL-STD-810H testedRoHS (Restriction of hazardous substances)WEEE (Waste electrical and electronic equipment)Russia EACChina CCCCompliance link https:///sites/certificatesAdditional SoftwareAdditional software (preinstalled)Adobe® Reader® (pdf reader)CyberLink YouCam (webcam software)Fujitsu Display ManagerFujitsu DeskUpdate (driver and utility tool)ShockSensor UtilityPower Saving UtilityFujitsu Plugfree Network (network management utility)EasyGuide online user documentationMicrosoft Office (1 month trial for new Microsoft® Office 365 customers. Buy Microsoft Office.)Additional software (optional)Recovery DVD for Windows®Drivers & Utilities DVD (DUDVD) optionalCyberLink PowerDVD BD (playback software for Blu-ray Disc™)CyberLink PowerDVD DVD (playback software for DVD)CyberLink YouCam (webcam software)Nero (backup and burning software)ManageabilitySecurityPhysical Security Kensington Lock supportSystem and BIOS Security Absolute Persistence® technology capable BIOSEraseDiskTrusted Platform Module (TPM 2.0)User Security Hard disk passwordUser and supervisor BIOS passwordEmbedded fingerprint sensor (optional)Smartcard reader (optional)SystemLock BIOS SmartCard securityAuthConductor Client Basic (secure authentication solution)Packaging informationWarranty Warranty period2 years (for countries within EMEIA)Warranty type Bring-in Service / Collect & Return Service (depending on country)Product Support - the perfect extension Recommended Service 9x5, Onsite Response Time: Next Business DaySpare Parts availabilityat least 5 years after shipment, for details see https:///Recommended AccessoriesThunderbolt™ 4 Port ReplicatorFirst Thunderbolt™ Port Replicator on the market providing enhancedsecurity and full support of Intel® AMT (vPro®).The universal port can easily connect almost everything with a single cable and high speed-data transfer. This smart workspace solution keeps your desk clean and tidy.Order Code: FPCPR401BPUSB Type-C Port Replicator 2Connect to your peripherals. Adapt to the task on demand. The universal USB Type-C interface supports you to get your peripheral devicesconnected easily. Multiply your USB ports to connect your peripherals as well as your external display via HDMI, DisplayPort or VGA.You also can charge your external USB devices without the need of any additional charger.Order Code:S26391-F3327-L100STYLISTIC Q7311, Q7310 andSTYLISTIC Q5010 Family Cradle Flexibility, expandability, desktop replacement, investment protection – to name just a few benefits of Fujitsu’s docking options.Order Code: S26391-F3397-L100USB Type-C AC AdapterRecharge your notebook or tablet at work, at home or on the road with this USB Type-C power source easily. Order Code: S26391-F3326-L502LAN Conversion Cable (USB toLAN) for STYLISTIC Q The FUJITSU USB Type-A to LAN Conversion Cable enables you to connect your compatible STYLISTIC Tablet to a wired network connection. GBit-LAN via USB conversion cable is limited to 480 Mbit/s due to USB 2.0specification. (Picture similar to product)Order Code:S26391-F3398-L840Tablet Stand for STYLISTIC QdevicesThe tablet stand is designed for STYLISTIC devices like latest STYLISTIC Q7312 and can be used universally. Fits for STYLISTIC Q5 and Q7 and the Type-C as well as the Thunderbolt ™ 4 port replicator can be fixed on the stand.Order Code: FPCSK769BPAES PenTake your creativity to the next level, with the most natural pen experience with the AES Pen with replacement stylus tips. With pen nibs that are very thin, you can experience the writing and signing behavior of an ink pen. The pen fits in the device’s integrated pen garage.Order Code:S26391-F3389-L500Car Adapter USB-C-QCOne Car Adapter fits all.Independent of your mobile device charging method this car adapter will fulfill all major requirements and standards for fast charging vendorbrand-independent notebooks and all mobile devices. The Car Adapter USB-C-QC supports USB PD with PPS, Quick Charge (QC), Apple- and Samsung charge.Order Code: S26391-F2613-L630CANVAS HANSEN 14The PLEVIER CANVAS HANSEN 14 leather and canvas case is a compact and classic carrier for on the go. Available for notebooks up to 14 inches with two compartments and two accessory sections, protection for your device ensured. A subtle design canvas and nappa leather shade.Order Code:S26391-F1193-L67Bumper Case KitThe protective bump case kit is designed to prevent damages to yourSTYLISTIC Q7. Openings of the cover allow you to access all ports andbuttons whilest the hand strap on the back of the case gives you a firm grip while working with your tablet outside the office. The integrated stand solution enhances your productivity.Order Code: S26391-F3396-L200Folio Cover STYLISTIC Q7311/Q7310The Folio Cover for the STYLISTIC Q7311 and Q7310 is a thin, tailored protective sleeve for your Fujitsu tablet. It keeps the device safe from damage on the go, and brings a touch of style to your daily work. It can even be used as a stand if you need to display a presentation or watch a clip and fits even the device is connected to its keyboard dock.Inclination stand style 120 to 150 degreeOrder Code:S26391-F3509-L100Wireless Mouse WI860 BTCThe Wireless Mouse WI860 BTC can be paired with up to 3 different clients, 2x Bluetooth and 1x wireless USB Type-C dongle.With the blue optical sensor, it works on nearly all surfaces with an 3-step adjustable DPI selector (800/1600/2400).The mouse charges wirelessly through Qi or by USB Type-C cable.A utility button on the side is programmable. The default functions are optimized for Teams calls.Order Code:S26381-K474-L100Order Code: FPCKG453BPContactAddress: x-xx-x, street, city, state, ZIP code, country Phone: xx-xxxx-xxxx Fax : xx-xxxx-xxxxEmail:********************.com Website: /[country]2023-08-02 CE-ENdelivery subject to availability. Any liability that the data and illustrations are complete, actual or correct is excluded. Designations may be trademarks and/or copyrights of the respective manufacturer, the use of which by third parties for their own purposes may infringe the rights of such ownerMore informationAll rights reserved, including intellectual property rights. Changes to technical data reserved. Delivery subject to availability. Any liability that the data and illustrations are complete, actual or correct is excluded.Designations may be trademarks and/or copyrights of the respective manufacturer, the use of which by third parties for their own purposes may infringe the rights of such owner.For further information see /terms_of_use.html Copyright © Fujitsu Technology Solutions。

SCALANCE X101-1 商品说明书

SCALANCE X101-1 商品说明书

24 V
6GK5101-1BB00-2AA3 Page 1/4
06/28/2018
Subject to change without notice © Copyright Siemens
● at DC / maximum
Supply voltage, current consumption, power loss Supply voltage ● external ● external Type of voltage / of the supply voltage Product component / fusing at power supply input Fuse protection type / at input for supply voltage Consumed current ● maximum Power loss [W] ● at DC / at 24 V
Compact 40 mm 125 mm 124 mm 0.55 kg
Yes Yes Yes
No No
FM3611: Class 1, Divison 2, Group A, B, C, D / T.., Class 1, Zone 2, Group IIC, T.. EN 600079-15 II 3 G EEx nA II T.. KEMA 06 ATEX 0021 X
Yes
Yes Yes Yes Yes Yes No 134 y
/snst
/simatic-net https:// /industry/infocenter /bilddb /cax https://
Security information
UL 60950-1, CSA C22.2 No. 60950-1 UL 1604 and UL 2279-15 (Hazardous Location), Class 1 / Division 2 / Group A, B, C, D / T.., Class 1 / Zone 2 / Group IIC / T.. EN 61000-6-3 EN 61000-6-4:2001 EN 61000-6-2:2001, EN 61000-6-4:2001 Yes Yes Yes

欧特本187411商品说明书

欧特本187411商品说明书

Eaton 187411Eaton Moeller series xEffect - FRCmM Type F RCCB. Residual current circuit breaker (RCCB), 80A, 4p, 30mA, type G/FGeneral specificationsEaton Moeller series xEffect - FRCmM Type F RCCB187411FRCMM-80/4/003-G/F401508182469480 mm 76 mm 70 mm 0.386 kg RoHS conformIEC/EN 62423 EN45545-2 IEC 61373 IEC/EN 61008Product NameCatalog Number Model CodeEANProduct Length/Depth Product Height Product Width Product Weight Compliances CertificationsFour-pole10 ms delayed80 A10 kA with back-up fuse 30 mAPulse-current sensitive3 kA (8/20 μs) surge-proof 240 V AC / 415 V AC240 V440 V4 kV0.03 A0.03 A50 Hz / 60 Hz80 A (max. admissible back-up fuse)Other800 A80 A gG/gL10 kA3 kA184 V AC - 440 V AC2Frequency mix (10 Hz, 50 Hz, 1000 Hz) enhanced sensitivity 4000 operationsApplicationNumber of polesTripping timeAmperage RatingRated short-circuit strength Fault current rating Sensitivity typeImpulse withstand current Type Voltage rating (IEC/EN 60947-2)Rated operational voltage (Ue) - maxRated insulation voltage (Ui)Rated impulse withstand voltage (Uimp) Rated fault current - minRated fault current - maxFrequency ratingShort-circuit ratingLeakage current typeRated residual making and breaking capacity Admissible back-up fuse overload - max Rated short-time withstand current (Icw) Surge current capacityTest circuit rangePollution degreeRadiation resistanceLifespan, electricalSwitchgear for industrial and advanced commercial applicationsxEffect - Switchgear for industrial and advanced commercial applicationsCurrent test marks as per inscriptionMaximum operating temperature is 75 °C: Starting at 40 °C, the max. permissible continuous current decreases by 1.2% for every 1 °C45 mm470 mm (4 SU)70.5 mmDIN railQuick attachment with 2 latch positions for DIN-rail IEC/EN 60715As requiredIP20, IP40 with suitable enclosureIP20White / blueTwin-purpose terminals1.5 mm² - 35 mm²1.5 mm²35 mm²16 mm² (2x)1.5 mm²16 mm²M5 (with cross-recessed screw as defined in EN ISO 4757-Z2, PZ2)80 A4.7 W18.8 W0 W0 W-25 °C40 °CMeets the product standard's requirements.Meets the product standard's requirements.Meets the product standard's requirements.Meets the product standard's requirements.Meets the product standard's requirements.Does not apply, since the entire switchgear needs to be evaluated.Does not apply, since the entire switchgear needs to be evaluated.FrameWidth in number of modular spacingsBuilt-in width (number of units)Built-in depthMounting MethodMounting positionDegree of protectionStatus indicationTerminals (top and bottom)Terminal capacity (solid wire)Connectable conductor cross section (solid-core) - min Connectable conductor cross section (solid-core) - max Terminal capacity (stranded cable)Connectable conductor cross section (multi-wired) - min Connectable conductor cross section (multi-wired) - max Terminal capacity (cable)Rated operational current for specified heat dissipation (In) Heat dissipation per pole, current-dependentEquipment heat dissipation, current-dependentStatic heat dissipation, non-current-dependentHeat dissipation capacityAmbient operating temperature - minAmbient operating temperature - max10.2.2 Corrosion resistance10.2.3.1 Verification of thermal stability of enclosures10.2.3.2 Verification of resistance of insulating materials to normal heat10.2.3.3 Resist. of insul. mat. to abnormal heat/fire by internal elect. effects10.2.4 Resistance to ultra-violet (UV) radiation10.2.5 Lifting10.2.6 Mechanical impact10.2.7 InscriptionsFinger and hand touch safe, DGUV VS3, EN 502742 Nm - 2.4 NmRed / green0.8 mm - 2 mm20000 operations-35 °C60 °C25-55 °C / 90-95% relative humidity according to IEC 60068-2Meets the product standard's requirements.Does not apply, since the entire switchgear needs to be evaluated.Meets the product standard's requirements.Does not apply, since the entire switchgear needs to be evaluated.Does not apply, since the entire switchgear needs to be evaluated.Is the panel builder's responsibility.Is the panel builder's responsibility.Is the panel builder's responsibility.Is the panel builder's responsibility.Is the panel builder's responsibility.The panel builder is responsible for the temperature rise calculation. Eaton will provide heat dissipation data for the devices.Is the panel builder's responsibility. The specifications for the switchgear must be observed.Is the panel builder's responsibility. The specifications for the switchgear must be observed.The device meets the requirements, provided the information in the instruction leaflet (IL) is observed.Terminal protectionTightening torqueContact position indicator colorBusbar material thicknessLifespan, mechanicalPermitted storage and transport temperature - min Permitted storage and transport temperature - max Climatic proofing 10.3 Degree of protection of assemblies10.4 Clearances and creepage distances10.5 Protection against electric shock10.6 Incorporation of switching devices and components 10.7 Internal electrical circuits and connections10.8 Connections for external conductors10.9.2 Power-frequency electric strength10.9.3 Impulse withstand voltage10.9.4 Testing of enclosures made of insulating material 10.10 Temperature rise10.11 Short-circuit rating10.12 Electromagnetic compatibility10.13 Mechanical functionFeatures Application notesEaton Corporation plc Eaton House30 Pembroke Road Dublin 4, Ireland © 2023 Eaton. All rights reserved. Eaton is a registered trademark.All other trademarks areproperty of their respectiveowners./socialmediaAdditional equipment possible Residual current circuit breakerInterlocking deviceShort-time delayed trippingType G/F (�VE E 8601) Residual current circuit breakers FRCmM eaton-rcd-application-guide-br019003en-en-us.pdfeaton-pdd-railrolling-stock-brochure-br011002en-en-us.pdf eaton-xeffect-frcmm-rccb-catalog-ca003018en-en-us.pdf DA-DC-03_FRCmMas_frcmmeaton-circuit-breaker-xeffect-frcmm-na-rccb-dimensions.epseaton-187407-drawing.dwgeaton-187407-3d-model.stpeaton-xeffect-frcmm-rccb-wiring-diagram-002.jpgFitted with: Functions Special featuresUsed with Brochures Catalogues Certification reports DrawingsmCAD model Wiring diagramsFRCmMResidual current circuit breakersType G/F (ÖVE E 8601)。

INIC3619_Datasheet_V1.00

INIC3619_Datasheet_V1.00
(INIC3619_Datasheet_V1.00.odt)
Revision 1.00 August 14, 2013 Initio Corporation
Revision 1.00
Page 1 of 28
INIC-3619 Datasheet _______________________________________________________________________________
批注本地保存成功开通会员云端永久保存去开通
INIC-3619 Datasheet _______________________________________________________________________________

INIC-3619 USB 3.0 SATA Bridge Datasheet
Contents
1 Introduction.......................................................................................................................................................................4 1.1 Feature Summary.......................................................................................................................................................4 1.2 Firmware Support..........................

1794各模块介绍

1794各模块介绍

、处理器1794-TBNF带接线端子的底座现货1794-TB32带接线端子的底座现货1794-TB3带接线端子的底座现货1794-PS3电源(220VAC3A)现货1794-PS13电源(220VAC1.3A)现货1794-OW88点继电器输出模块现货1794-OE44点模拟量输出模块现货1794-OB824VDC8点输出模块现货1794-OB32P24VDC32点输出模块现货1794-OB1624VDC16点输出模块现货1794-IT88点温度输入模块现货1794-IR88点RTD输入模块现货1794-IE88点模拟量输入模块现货1794-IB824VDC8点输入模块现货1794-IB3224VDC32点输入模块现货1794-IB1624VDC16点输入模块现货1794-AENTEtherNet网络适配器现货1794-ADNDeviceNet网络适配器现货1794-ACNR15ControlNet网络冗余适配器现货1794-ACN15ControlNet网络适配器现货FLEX数字量直流输出模块*舉洁佶n率之空仙陽工苴rttFLEX数字量直流输入模块FLEX数字量直流组合模块目鵜输入输出输入点数导通状态输入电压工作电压范围缺省信号延迟断开状态输出点数每个模块的最大电流端子基座*Flexbus总线电流(mA)外接H流毓最大功耗1794-IB10xOB6 5路灌入型24V宣流19.2-3X2V直流0.25ms1.5mA6路拉出型200mA 1794-TB3,1794-35mA 24V直流时31.2V直流1794-IB16xOB16P 16路灌入型24V宜流10-3他直流0.25ms1.5mA16路拉出型,带保500mA 1794-TB32,-TB32S80mA 24V直流时31.2VA流W7.0W擋存使用黒体文宁的端于*座FLEX数字量触点式输出模块目鵜输出点数工作电压范围每路输出的最大电流每个模块的最大电流端子基座*Flexbus总线电流(mA)外接直流畅最大电流最大功耗1794-OW8 8路隔高常开型继5-240V直流2測交能时3A带电阻16A1794-TBNF 70mA 125mA 31.2VX流讨5.0W整个系统中分布式I/O采用Rockwell公司生产的FlexLogix系列模块,分布式I/O系统由控制器、I/O模块、通信模块、电源、端子型I/O基座、安装导轨组成。

Micrel KSZ8851-16MLL 48-pin单端口以太网控制器与非PCI接口评估板用户指南

Micrel KSZ8851-16MLL 48-pin单端口以太网控制器与非PCI接口评估板用户指南

KSZ8851-16MLL48-pin Single-Port Ethernet ControllerWith 8-bit or 16-bit Non-PCI InterfaceEvaluation Board User’s GuideRevision 1.1August 2010© Micrel, Inc. 2007All rights reservedMicrel is a registered trademark of Micrel and its subsidiaries in theUnited States and certain other countries. All other trademarks are theproperty of their respective owners.The information furnished by Micrel in this datasheet is believed to be accurate and reliable. However, no responsibility is assumed by Micrel for its use. Micrel reserves the right to change circuitry and specifications at any time without notification to the customer. Micrel Products are not designed or authorized for use as components in life support appliances, devices or systems where malfunction of a product can reasonably be expected to result in personal injury. Life support devices or systems are devices or systems that (a) are intended for surgical implant into the body or (b) support or sustain life, and whose failure to perform can be reasonably expected to result in a significant injury to the user.Micrel, Inc. August 6, 2010A Purchaser's use or sale of Micrel Products for use in life support appliances, devices or systems is at Purchaser's own risk and Purchaser agrees to fully indemnify Micrel for any damages resulting from such use or sale.Revision HistoryRevisionDateSummary of Changes1.0 2/20/2008 Initial Release1.1 8/6/2010Add JP8 for connection between VDD_IO and 1.8V (if VDD_IO is 1.8V). Changed the LED pulled up to 3.3V.Table of Contents1.0Introduction (5)2.0Board Features (5)3.0Evaluation Kit Contents (5)4.0Hardware Description (6)4.1Host Interface (7)4.2Jumper Setting & Definition (8)4.3Power Supply and Test Point Definition (9)4.4RJ-45 Connector and Transformer (9)4.5EEPROM and LED Indicators (9)4.6Board Reset (10)5.0Bill of Materials (11)Micrel, Inc. August 6, 2010List of FiguresFigure 1. KSZ8851-16MLL Evaluation Board (6)Figure 2. KSZ8851-16MLL-Eval Host Interface Connection with Spirent SmartBits (7)List of TablesTable 1. Header JP1 – Host Interface Connection for SD[15:0] (7)Table 2. Header JP1 – Host Interface Connection for Control and Power (8)Table 3. KSZ8851-16MLL-Eval Jumper Definition (8)Table 4. KSZ8851-16MLL-Eval Test Point Definition (9)Table 5. KSZ8851-16MLL EEPROM Format (9)Table 6. KSZ8851-16MLL-Eval Port Status LED Definition (10)Table 7. KSZ8851-16MLL-Eval LED Definition (10)Micrel, Inc. August 6, 20101.0 IntroductionThe KSZ8851-16MLL-Eval Evaluation Board is intended to provide a convenient and fast way to evaluate or demonstrate the functionality and performance of this new Single-Port Ethernet Controller KSZ8851-16MLL device from Micrel.The KSZ8851-16MLL comes with a 48-pin, lead-free LQFP (7mm x 7mm) package and provides an ideal solution for applications requiring high-performance from single-port Ethernet Controller with 8-bit or 16-bit generic processor interface. The KSZ8851-16MLL offers the most cost-effective solution for adding high-throughput Ethernet connectivity to traditional embedded systems.This evaluation board is designed as a stand alone without microcontroller or M16C on board. By default the KSZ8851-16MLL-Eval board comes with an operation of 16-bit bus mode, Little Endian mode and disabled EEPROM for KSZ8851-16MLL device. Customer may wire the board for his desired interface. The purpose is to provide a simple tool that can be used to evaluate the KSZ8851-16MLL device by connecting via headers to customer provided Microcontroller or Non-PCI hardware platform.Micrel provides a basic software driver based on the 8 or 16-bit bus solution and different operating system platforms to evaluate the KSZ8851-16MLL functionality and performance. The software includes a configuration utility to allow quick and easy device setup, initialization and transmit/receive packet. All KSZ8851-16MLL configuration pins and host interface signals are accessible either by jumpers, test points or headers.2.0 Board Features•One KSZ8851-16MLL 48-pin Single-Port Ethernet Controller with shared data bus for host interface•Single +5V/GND power input from headers•RJ-45 Jack for Fast Ethernet cable interface•HP Auto-MDIX for automatic detection and correction for straight-through and crossover cables•Two on board LDO voltage regulators, one for VDD_IO and the other for VDD_A3.3•One AT93C46 for external EEPROM interface•Two LED indicators for port status and activity•One LED indicator for 3.3V output ready•One LED indicator for Power Management Event (PME) output status•Jumpers to configure strapping pins and VDD_IO voltage option•Headers to wire the host interface from external hardware platform•Manual reset button for quick reboot after re-configuration of strapping pins3.0 Evaluation Kit ContentsThe KSZ8851-16MLL Evaluation Kit includes the following hardware:•KSZ8851-16MLL Evaluation BoardThe KSZ8851-16MLL Data Sheet and Hardware Design Package with the following collaterals that can be downloaded from Micrel’s website at •KSZ8851-16MLL Eval Board Schematic (PDF and OrCAD DSN file)•KSZ8851-16MLL Eval Board Gerber File (PDF version included)•KSZ8851-16MLL Eval Board User’s Guide (this document and included BOM)•KSZ8851-16MLL IBIS ModelMicrel, Inc. August 6, 20104.0 Hardware DescriptionThe KSZ8851-16MLL-Eval (shown in Figure 1) comes in a compact form factor and plugs directly into industry standard test equipment such as Spirent SmartBits, the other side of board is wired to external host interface through headers. Configuration of the KSZ8851-16MLL is accomplished through on-board jumper selections and/or by register access via the host shared data/control bus Interface.Figure 1. KSZ8851-16MLL Evaluation Board (Rev 1.1)Other features include a RJ-45 Jack for Fast Ethernet cable connection, transformer (Pulse H1102) to block DC level and provide a true AC coupling, EEPROM (Atmel AT93C46) to load MAC address when it is enabled, jumper to select LDO output for VDD_IO voltage, programmable LED indicators for reporting port link status and activity, and a manual reset button for quick reboot after re-configuration of strapping pins.The KSZ8851-16MLL-Eval receives +5V DC input power supply from its Headers JP1.Micrel, Inc. August 6, 2010Micrel, Inc. August 6, 20104.1 Host InterfaceThe KSZ8851-16MLL-Eval board receives +5V power from the header JP1 (pin 1/3). Figure 2 shows the Host interface connection with Spirent SmartBits for system set-up and performance test.Figure 2. KSZ8851-16MLL-Eval Host Interface Connection with Spirent SmartBitsThe KSZ8851-16MLL-Eval has a 40-pin header (JP1) for Host interface to external any Non-PCI hardware platform. Table 1 lists Shared Data SD[15:0] pin outs for the Host interface on header JP1. Table 2 lists the rest of control signals and power/ground pin outs for the Host interface on header JP1.16-Bit Bus Mode (pin 1 pull-up) 8-Bit Bus Mode (pin 1 pull down) Pin # (JP1) Shared Data Bus Signal CMD = 0 (low) CMD = 1 (high) CMD = 0 (low) CMD = 1 (high) 20 SD0 D0 x (don’t care) D0 A0 19SD1D1 x (don’t care) D1 A1 18 SD2 D2 A2 D2 A2 17 SD3 D3 A3 D3 A3 16 SD4 D4 A4 D4 A4 15 SD5 D5 A5 D5 A5 14 SD6 D6 A6 D6 A6 13 SD7 D7 A7 D7 A7 12 SD8 D8 x (don’t care) GND GND 11 SD9 D9 x (don’t care) GND GND 10 SD10 D10 x (don’t care) GND GND 9SD11D11 x (don’t care) GND GND 8 SD12 D12 BE0 GND GND 7 SD13 D13 BE1 GND GND 6 SD14 D14 BE2 GND GND 5 SD15D15BE3GNDGNDTable 1. Header JP1 – Host Interface Connection for SD[15:0]Pin # (JP1) Power & ControlSignal NamesDescription1, 3 5.0V_IN +5V power supply inputs for this board 2, 4, 21, 22, 25,26, 29, 33, 34, 37, 38, 39, 40 GND Groundinputs/pins23 CPU_CSN Chip Select input from host CPU24 CPU_RSTN Reset input from host CPU27 CPU_PME Power Management Event output to host CPU28 CPU_CMD Command type input from host CPU31 CPU_INTRN Interrupt output to host CPU35 CPU_WRN Write input from host CPU36 CPU_RDN Read input from host CPU30, 32 Spares For customer to useTable 2. Header JP1 – Host Interface Connection for Control and Power4.2 Jumper Setting & DefinitionThe KSZ8851-16MLL-Eval does not require any jumper for normal operation except the VDD_IO option. During power-up, the KSZ8851-16MLL is configured using the chip’s internal pull-up and pull-down resistors with its default strapping pin values which will set this device in operation of 16-bit bus mode, little endian and without EEPROM. Jumpers are provided to override the default settings, allowing for quick configuration and re-configuration of the board. To override the default settings, simply select and close the desired jumper setting(s) and toggle the on-board manual reset button (S1) for the new setting(s) to take effect.The KSZ8851-16MLL-Eval jumper settings are defined in Table 3 below.Jumper Definition Setting DescriptionJP2 EED_IO OFF (Default) OFF: EEPROM is not presentON: EEPROM is presentJP3 3.3V ON (Default) ON: to select 3.3V for VDD_IO (JP5 and JP6 must be OFF)OFF: De-select 3.3VJP4 EESK OFF (Default) OFF: Little EndianON: Big EndianJP5 2.5V OFF (Default) ON: to select 2.5V for VDD_IO (JP3 and JP6 must be OFF)OFF: De-select 2.5VJP6 1.8V OFF (Default) ON: to select 1.8V for VDD_IO (JP3 and JP5 must be OFF)OFF: De-select 1.8VJP7 P1LED1 OFF (Default) OFF: 16-Bit bus modeON: 8-Bit bus modeJP8 VDD_IO OFF (Default) OFF: VDD_IO = 2.5V or 3.3VON: VDD_IO = 1.8VTable 3. KSZ8851-16MLL-Eval Jumper DefinitionMicrel, Inc. August 6, 2010Power Supply and Test Point DefinitionThe KSZ8851-16MLL-Eval is supplied from external +5.0V DC power through a jumper (pin 1 and 3 at JP1), this +5.0V DC input is converted to both +3.3V with a Micrel LDO voltage regulator (U3, MIC5209BM) for VDD_A3.3 analog power and VDD (option for 3.3V, 2.5V or 1.8V) with a Micrel LDO voltage regulator (U5, MIC5209BM) for VDD_IO digital power. The KSZ8851-16MLL contains an internal +1.8V LDO, to provide its core, analog and PLL voltages.The KSZ8851-16MLL-Eval has four test points. They are defined in the following Table 4.Test Point DefinitionTP1 Power supply measurement for VDD_IOTP2 Power supply measurement for VDD_A3.3TP3 1.8V digital core voltage output measurement fromKSZ8851-16MLL internal LDOTP4 External power supply 5.0V_IN measurementTable 4. KSZ8851-16MLL-Eval Test Point Definition4.3 RJ-45 Connector and TransformerThe RJ-45 Jack (J1) connects to standard CAT-5 Ethernet cable to interface with 10Base-T/100Base-TX Ethernet devices. The LAN interface on the KSZ8851-16MLL is connected to a transformer (T1) with 50 ohm termination resistors for both TX+/- and RX+/- differential pairs. The line side of the transformer is connected to the RJ-45 connectors (J1).J1 also supports Auto-MDIX and Auto-Negotiation / Forced Modes.4.4 EEPROM and LED IndicatorsIt is optional in the KSZ8851-16MLL to use an external EEPROM. The EED_IO (JP2) must be pulled high (ON) to use external EEPROM.An external serial EEPROM with a standard microwire bus interface is used for non-volatile storage of information such as the host MAC address. The KSZ8851-16MLL can detect if the EEPROM is either a 1KB (93C46) or 4KB (93C66) EEPROM device. The EEPROM must be organized as 16-bit mode.The KSZ8851-16MLL EEPROM format is given in Table 5.WORD 15 8 7 00H Reserved1H Host MAC Address Byte 2 Host MAC Address Byte 12H Host MAC Address Byte 4 Host MAC Address Byte 33H Host MAC Address Byte 6 Host MAC Address Byte 54H – 6H Reserved7H-3FH Not used for KSZ8851-16MLL (available for user to use)Table 5. KSZ8851-16MLL EEPROM FormatMicrel, Inc. August 6, 2010A dual LED indicator (LED1) is located adjacent to the RJ-45 Connector (J1). The top LED is connected to P1LED1 (pin 1) and bottom LED is connected to P1LED0 (pin 2) of the KSZ8851-16MLL.The two LEDs are programmable to LED mode ‘0’ or ‘1’ via register 0xC6 bits [9], and are defined in the following Table 6.LED Mode0 (Default) 1LED1 (Top) 100BT ACTLED1 (Bottom) LINK/ACT LINKTable 6. KSZ8851-16MLL-Eval Port Status LED DefinitionTable 7 shows the rest of LEDs definition.LED Color DescriptionLED2 Green Power Management Event (PME) StatusLED3 Red 3.3V Power available indicatorTable 7. KSZ8851-16MLL-Eval LED Definition4.5 Board ResetThe KSZ8851-16MLL-Eval generates a reset signal from the reset circuitry during power up. It also provides a push button S1 reset circuit to reset the KSZ8851-16MLL device. During power up, the board is automatically reset. User can also press reset button S1 on the board for a manual reset.Micrel, Inc. August 6, 2010KSZ8851-16MLL-Eval Evaluation Board User’s Guide Rev. 1.1 Micrel, Inc.August 6, 201011/11 5.0 Bill of MaterialsKSZ8851-16MLL Eval Board (Revision 1.1)。

Connect Tech XMC to PCIe 104 Adapter 用户指南说明书

Connect Tech XMC to PCIe 104 Adapter 用户指南说明书

XMC to PCIe/104 AdapterConnect Tech Inc.Tel:519-836-129142 Arrow Road Toll:800-426-8979 (North America only) Guelph, Ontario Fax:519-836-4878N1K 1S6 Email:********************************************Table of ContentsTable of Contents (2)Preface (3)Disclaimer (3)Customer Support Overview (3)Contact Information (3)Limited Product Warranty (4)Copyright Notice (4)Trademark Acknowledgment (4)ESD Warning (5)Revision History (5)Introduction (6)Product Features and Specifications (6)Product Overview (7)Connector Summary & Locations (7)Jumper Summary & Locations (8)Detailed Feature Description (9)PCIe/104 Connector (9)Description (9)Connectors & Jumpers (9)XMC Expansion Slot (10)Description (10)Connectors & Jumpers (10)Indicator LEDs (11)Description (11)Connectors & Jumpers (11)Typical Installation (12)PrefaceDisclaimerThe information contained within this user’s guide, including but not limited to any product specification, is subject to change without notice.Connect Tech assumes no liability for any damages incurred directly or indirectly from any technical ortypographical errors or omissions contained herein or for discrepancies between the product and the user’s guide.Customer Support OverviewIf you experience difficulties after reading the manual and/or using the product, contact the Connect Tech reseller from which you purchased the product. In most cases the reseller can help you with product installation and difficulties.In the event that the reseller is unable to resolve your problem, our highly qualified support staff can assist you.Our support section is available 24 hours a day, 7 days a week on our website at:/sub/support/support.asp. See the contact information section below for moreinformation on how to contact us directly. Our technical support is always free.Contact InformationMail/CourierConnect Tech Inc.Technical Support42 Arrow RoadGuelph, OntarioCanada N1K 1S6Email/Internet********************************************Telephone/FacsimileTechnical Support representatives are ready to answer your call Monday through Friday, from 8:30 a.m. to 5:00 p.m. Eastern Standard Time. Our numbers for calls are:Toll Free: 800-426-8979 (North America only)Telephone: 519-836-1291 (Live assistance available 8:30 a.m. to 5:00 p.m. EST,Monday to Friday)Facsimile: 519-836-4878 (on-line 24 hours)Limited Product WarrantyConnect Tech Inc. provides a 2 year Warranty for the XMC to PCIe/104 Adapter. Should this product, in Connect Tech Inc.'s opinion, fail to be in good working order during the warranty period, Connect Tech Inc.will, at its option, repair or replace this product at no charge, provided that the product has not been subjected to abuse, misuse, accident, disaster or non-Connect Tech Inc. authorized modification or repair.You may obtain warranty service by delivering this product to an authorized Connect Tech Inc. business partner or to Connect Tech Inc. along with proof of purchase. Product returned to Connect Tech Inc. must be pre-authorized by Connect Tech Inc. with an RMA (Return Material Authorization) number marked on the outside of the package and sent prepaid, insured and packaged for safe shipment. Connect Tech Inc. will return this product by prepaid ground shipment service.The Connect Tech Inc. Limited Warranty is only valid over the serviceable life of the product. This is defined as the period during which all components are available. Should the product prove to be irreparable, Connect Tech Inc. reserves the right to substitute an equivalent product if available or to retract the Warranty if no replacement is available.The above warranty is the only warranty authorized by Connect Tech Inc. Under no circumstances willConnect Tech Inc. be liable in any way for any damages, including any lost profits, lost savings or otherincidental or consequential damages arising out of the use of, or inability to use, such product. Copyright NoticeThe information contained in this document is subject to change without notice. Connect Tech Inc. shall not be liable for errors contained herein or for incidental consequential damages in connection with the furnishing, performance, or use of this material. This document contains proprietary information that is protected by copyright. All rights are reserved. No part of this document may be photocopied, reproduced, or translated to another language without the prior written consent of Connect Tech, Inc.Copyright 2016 by Connect Tech, Inc.Trademark AcknowledgmentConnect Tech, Inc. acknowledges all trademarks, registered trademarks and/or copyrights referred to in this document as the property of their respective owners. Not listing all possible trademarks or copyrightacknowledgments does not constitute a lack of acknowledgment to the rightful owners of the trademarks and copyrights mentioned in this document.ESD WarningElectronic components and circuits are sensitive toElectroStatic Discharge (ESD). When handling any circuit board assemblies including Connect Tech COM Express carrier assemblies, it is recommended that ESD safety precautions be observed. ESD safe best practices include, but are not limited to:∙ Leaving circuit boards in their antistatic packaginguntil they are ready to be installed.∙ Using a grounded wrist strap when handling circuitboards, at a minimum you should touch a grounded metal object to dissipate any static charge that may be present on you.∙ Only handling circuit boards in ESD safe areas, whichmay include ESD floor and table mats, wrist strap stations and ESD safe lab coats.∙ Avoiding handling circuit boards in carpeted areas. ∙ Try to handle the board by the edges, avoiding contactwith components.Revision HistoryIntroductionConnect Tech’s XMC to PCIe/104 Adapter Board is an engineering tool for the purpose of enabling rapid development of systems requiring the use of next generation form factor peripheral cards. This product complies with the VITA 42 specification.Product Features and SpecificationsProduct Overview Connector Summary & Locations ADG095 Top ViewADG095 BottomViewJumper Summary & LocationsDetailed Feature DescriptionPCIe/104 ConnectorDescriptionPCIe/104 interface to CPU module Connectors & JumpersXMC Expansion SlotDescriptionM.2 interface slots for expansion cards. Can be ordered in either key E or key BM. Card type support is listed below.See Part Numbers/Ordering Information section for more ordering details.Connectors & JumpersIndicator LEDsDescriptionIndicator LED’s Connectors & JumpersTypical InstallationCard may be installed in a stack-up or stack-down configuration. 10mm height, M2.5 standoffs required between XMC adapter and XMC expansion card. Example stack-up on Connect Tech’s Com Express Type 6 104e Carrier shown below:。

广州爱普电子技术有限公司产品说明书

广州爱普电子技术有限公司产品说明书

Typical Features◆Wide input voltage range:85-265VAC/120-380VDC◆No-load power consumption≤≤0.5W◆Transfer efficiency(typ.87%)◆Switching frequency:65KHz◆Protection:Short Circuit,Over Current◆Isolation voltage:2500Vac◆Plastic case,conform to UL94V-0Class◆PCB mountingApplication FieldFA24-220SXXG3N3Series-----a compact size,high efficient power converter offered by Aipu.It features universal input voltage,DC and AC dual-use,low ripple,low temperature rise,low power consumption, high efficiency,high reliability,safer isolation,with good EMC performance.EMC and Safety standard meet international EN55032,IEC/EN61000.It widely used in power,industrial,instrument,smart home applications.For harsh EMC environment,the application circuit in the datasheet is strongly recommended.Typical Product ListPart No.Output SpecificationMax.Capacitive LoadRipple&Noise20MHz(Max)Efficiency@Full Load220Vac(Typical) Power Voltage1Current1Voltage2Current2(W)Vo1(V)Io1(m A)Vo2(V)Io2(m A)u F mVp-p%FA24-220S12G3N32412.02000--200015085 FA24-220S15G3N32415.01600--20008086 FA24-220S24G3N324241000--80010087 Note1:Ripple&Noise of FA24-220S15G3N3,FA24-220S24G3N3should be tested with EMC solution recommended circuit,please see photo1at back.Note2:Due to space limitations,above is only a part of our product list,please contact our sales team for more items.Note3:.”*”is model under developing.Note4:The typical output efficiency is based on that product is full loaded and burned-in after half an hour.Note5:The fluctuation range of full load efficiency(%,TYP)is±2%,full load output efficiency=total output power/module’s input power.Input SpecificationItem Operating Condition Min.Typ.Max.UnitInput Voltage Range AC Input85220265VAC DC Input120310380VDCInput Frequency Range-475063HzInput Current 115VAC//250mA 220VAC//150Surge Current 115VAC//10 220VAC//20Leakage Current-0.5mA TYP/230VAC/50HzExternal fuserecommended value-2A-5A/250VAC slow-fusing Hot plug-UnavailableRemote control terminal-UnavailableOutput SpecificationItem Operating Condition Min.Typ.Max.UnitVoltage Accuracy Full input voltagerangeAny loadVo1--±2.0%Vo2---%Line Regulation Nominal Load Vo1--±2.0% Vo2---%Load RegulationNominal inputVoltage20%~100%loadVo1--±2.0%Vo2---%No load power consumption Input115VAC--0.5W Input220VAC--Minimum loadSingle Output0--% Positive Negative Dualoutput commongrounded---% Positive Negative Dualoutput isolated---Turn-on Delay Time Nominal input voltage,fullload-300-mSPower-off Holding Time Input115VAC(full load)-65-mS Input220VAC(full load)--Output Overshooting Full input voltage range(full load)--10%Dynamic Response 25%~50%~25%50%~75%~50%Overshoot range(%):≤±5%%Recovery time(mS):≤5.0mS mSShort Circuit Protection Input full voltage range Continuous,Self-recovery Hiccup Drift Coefficient--±0.03%-%/℃Over Current Protection Input220VAC≥120%Io,Self-recovery HiccupRipple&Noise Vo=12.0V≤150mV Vo=15.0V≤80mVVo=24.0V≤100Note:Ripple&Noise is tested by Twisted Pair Method,details please see Ripple&Noise Test at back. General SpecificationsItem Operating Condition Min.Typ.Max.Unit Switching Frequency-606570KHz Operating Temperature--40-+75℃Storage Temperature--40-+85Relative Humidity-10-90%RHIsolation Voltage Input-Output,Test1min,leakage current≤5mA2500--VACInsulation Resistance Input-Output@DC500V100--MΩMTBF-≥300,000H@25℃Vibration-10-55Hz,10G,30Min,alongX,Y,ZClass of Case Material-UL94V-0EMC CharacteristicsTotal Item Sub Item Test Standard ClassEMC EMICE CISPR22/EN55032CLASS B(see recommended circuit Photo2)RE CISPR22/EN55032CLASS B(see recommended circuit Photo2)EMSRS IEC/EN61000-4-310V/m Perf.Criteria BCS IEC/EN61000-4-63Vr.m.s Perf.Criteria BESD IEC/EN61000-4-2Contact±4KV/Air±8KV Perf.Criteria BSurge IEC/EN61000-4-5±1KV Perf.Criteria B(see recommendedcircuit Photo2)EFT IEC/EN61000-4-4±2KV Perf.Criteria BVoltage dips,shortinterruptions and voltagevariations immunityIEC/EN61000-4-110%~70%Perf.Criteria BPacking DimensionPacking Code L x W x HG339.0x25.0x22.0mmPin DefinitionPin-out12345Single(S)AC(N)AC(L)GND NP+VoNote:If the definition of pin is not in accordance with the model selection manual,please refer to the label on actual item. Ripple&Noise Test:(Twisted Pair Method20MHZ bandwidth)Test Method:(1)12#twisted pair to connect,Oscilloscope bandwidth set as20MHz,100M bandwidth probe,terminated with0.1uFpolypropylene capacitor and10uF high frequency lowresistance electrolytic capacitor in parallel,oscilloscope set asSample pattern.(2)Input terminal connect to power supply,output terminalconnect to electronic load through jig plate,Use30cm±2cmsampling line.Power line selected from correspondingdiameter wire with insulation according to the flow of outputcurrent.Product Characteristic CurveNote1:Input Voltage should be derated base on Input Voltage Derating Curve when it is85~100VAC/240~265VAC/120~140VDC/340~380VDC.2:Our product is suitable to use under natural air cooling environment,if use it under closed condition,please contact with us. Typical EMC Circuit and Recommended Spec1.Typical Application CircuitPart No.CE1L1CE2TVS1FA24-220S12G3N3NC2uH470uF/16V SMBJ14.0AFA24-220S15G3N3220uF/25V5uH220uF/25V SMBJ17.0A*FA24-220S24G3N3220uF/35V5uH220uF/35V SMBJ26.0ANote:Output filter capacitor C2is electrolytic capacitor,recommend high frequency low resistor electrolytic capacitor,for capacity and current low,please refer to the technical specifications provided by each manufacturer.C2capacitor withstand voltage should derate to80%,capacitor C1is ceramic capacitor,to filter high frequency noise,recommended0.1uF/50V/1206.TVS1tube is a recommend component to protect post-circuit if converter fails.Recommend to external FUSE,Model:3.15A/250V, slow fusing.2.EMC solution recommended circuitPhoto2,EMC for higher requirement circuitComponent Products Module ValueFUSE 3.15A/250Vac 3.15A/250Vac,slow-fusing,necessaryNTC5D-95D-9MOV10D561K10D561KCX10.47uF/275Vac0.47uF/275VacL1 6.8uH/3.0A 6.8uH/3.0A H inductorLF2UU9.830mH min30mH/3.0ANote:1.The product should be used under the specification range,otherwise it will cause permanent damage to it.2.Product’s input terminal should connect to fuse;3.If the product is not worked under the load range(below the minimum load or beyond the load range),we cannot ensure that the performance of product is in accordance with all the indexes in this manual;4.Unless otherwise specified,data in this datasheet are tested under conditions of Ta=25℃,humidity<75%when inputting nominal voltage and outputting rated load(pure resistance load);5.All index testing methods in this datasheet are based on our Company’s corporate standards6.The performance indexes of the product models listed in this manual are as above,but some indexes of non-standard model products will exceed the above-mentioned requirements,please directly contact our technician for specific information;7.We can provide customized product service;8.The product specification may be changed at any time without prior notice.。

卡洛加吉电子有限公司产品:无线困扰保护设备说明书

卡洛加吉电子有限公司产品:无线困扰保护设备说明书

Product DescriptionWireless Entrapment Pro-tection Device (EPD) for Industrial Gates. The system is designed to replace the connection cable between the ESPE (electro-sensitive protective equipment) and the gate controller. The sub-controller has input for either N.C. ESPE, N.O. 8.2 kΩ ESPE or the Carlo Gavazzi low con-sumption photoelectric ESPE N.C. contact. The system is designed for high reliabilityusing 2.4 GHz duplex com-munication between the main controller and sub controller. The main controller can han-dle up to 6 sub controllers i.e. one system can handle 12 ESPEs.The active time is initiated with a test signal applied on the main controller. The dura-tion of this can be fixed or defined by the length of the test signal.• Wireless Entrapment Protection Device for Industrial Gates• Input for two ESPE (electro-sensitive protective equipment)• Replaces cable between Gate controller and ESPE • Output ESPE: 2 x SPST NC or 2 x SPST NO (8,2kΩ) or 2 x Photoelectric sensors• Output low battery: 1 x SPST NC or NO • 2,4 GHz duplex communication • Built-in antenna • IP66 ratingsWireless Entrapment Protection Device For Industial Gates ESPEType WSM / WSS …Specifications Main Controller (WSM)Type SelectionHousing Range Output Type Ordering no.W x H x D Wireless75 x 125 x 35 mm 15 m NO 8K2 Main Controller WSM6GAOOD2475 x 125 x 35 mm 15 m NC Main Controller WSM6GACCD2445 x 214 x 22 mm 15 m-Sub Controller WSS2GA2BAT Housing Range Cable length TypeOrdering no. W x H x D S n Photoelectric Sensor Ø11 x 24.5 mm 2.5 m 2 m Emitter PB 11 CNT 15 WE Ø11 x 24.5 mm2.5 m12 mReceiverPB 11 CNT 15 WRICWSM/WSS ...Specifications Main Controller (WSM) (cont.)Specifications Sub Controller (WSS)NOTE: Changes/modifications not approved by Carlo Gavazzi could void the user’s authority to operate the equipment.WSM/WSS ...Specifications Photoelectric Sensors ESPE (PB11)NOTE: Changes/modifications not approved by Carlo Gavazzi could void the user’s authority to operate the equipment.Specifications Sub Controller (WSS) (cont.)(R&TTE) Directive 1999/5/EC Low Voltage Directive 2006/95/ECElectromagnetic Compatibility Directive 2004/108/EC Machinery Directive 2006/42/EC, amended by Directive 98/79/ECFor industrial doors onlySee EN13241-1Operation Diagram: Fixed Active TimeWSM/WSS ...Excess Gain PB111101001000E x c e s s g a i n110Distance (m)1003,332,8328,1(feet)Operation Diagram: Manual Active TimeWSM/WSS ...Detection Diagram PB11-60-40-202040600246810126,613,119,726,332,839,4Sensing range (m)(m )0(Feet)-196,9-131,2-65,665,6131,2196,90(F e e t )Wiring DiagramsYXEmitterReceiverLifetime of batteriesActive Duration in seconds3 Sub ctrl., 2 x optical sensors12 Cycles/day 25 Cycles/day 50 Cycles/day 100 Cycles/day 200 Cycles/day 400 Cycles/dayY e a r s0,00,40,20,60,81,01,21,41,61530604575901050,00,40,20,60,81,01,21,41530604575901053 Sub ctrl., 2 x Mech NO-8K2Active Duration in secondsY e a r sActive Duration in seconds 1 Sub ctrl., 2 x optical sensors12 Cycles/day 25 Cycles/day 50 Cycles/day 100 Cycles/day 200 Cycles/day 400 Cycles/dayY e a r s0,00,40,20,60,81,01,21,61,41530604575901050,00,40,20,60,81,01,21,41530604575901051 Sub ctrl.,2 x Mech. NO-8K2Active Duration in secondsY e a r sConditions: 240 working days, ambient temperature 20°C, 85% battery efficiency. ”Duration” is gate opening or closing time. One cycle is an opening AND closing cycle.4 x ER14505 3.6 VDC size AA, ≥ 2700 mAh Lithium batteriesNormal speedFast speedWSM/WSS ...WSM/WSS ... DimensionsDelivery Contents Main Controller •Wireless Main Controller: WSM6GAOOD24 orWSM6GACCD24• Manual•4 x SCREW M4.5X45 MM BOSSARD BN615•4 x RAWLPLUG SX8 NYLON• Packaging: Cardboard box Delivery Contents Sub Controller •Wireless Sub Controller: WSS2GA2BAT• Manual•2 x ER14505 3.6 VDC size AA, ≥ 2700 mAh Lithium batteries•4 x SCREW M4X10 MM BOSSARD BN1023• Packaging: Cardboard boxDelivery Contents Receiver •Receiver: PB11CNT15WR• Packaging: Plastic bag Delivery Contents Emitter •Emitter: PB11CNT15WE• Packaging: Plastic bag。

414H;中文规格书,Datasheet资料

414H;中文规格书,Datasheet资料

/
414 H
DC axial compact fan
Technical features
General description Particular design features: Compact fan with low power consumption. Some models suitable for use at high ambient temperatures. General features: Material: fibreglass-reinforced plastic. Impeller PA, housing PBT. Electronic commutation completely integrated. Protected against reverse polarity and locking. Connection via single strands AWG 28, TR 64, bared and tin-plated. Air exhaust over bars. Direction of rotation counter-clockwise seen on rotor. Masse: 17 g. 0.027 kg 40 x 40 x 20 mm Fiberglass-reinforced PA plastic Fiberglass-reinforced PBT plastic Air exhaust over bars Left, looking at rotor Sintec sleeve bearing 45000 h 15000 h Single strands AWG 28, TR 64, bared and tin-plated. Protected against reverse polarity and locking. VDE, CSA, UL

美国爱本电子(Eaton)产品说明书

美国爱本电子(Eaton)产品说明书

Eaton PDG34FH400P2DJPower Defense Globally Rated, Frame 3, Four Pole, 400A-High, 25kA/480V, PXR25 LSI w/ Modbus RTU, CAM Link and Relays, Std Line Load Term (PDG3X4TA401H)Eaton Power Defense molded case circuit breakerPDG34FH400P2DJ 786679538265109.1 mm 257.1 mm 182.9 mm 7.68 kg Eaton Selling Policy 25-000, one (1) year from the date of installation of theProduct or eighteen (18) months from thedate of shipment of the Product,whichever occurs first.RoHS Compliant UL 489IEC 60947-2CCC MarkedCSAProduct NameCatalog Number UPCProduct Length/Depth Product Height Product Width Product Weight WarrantyCompliancesCertifications400A High Override Complete breaker 3Four-pole (100% N)PD3 GlobalClass APXR 25 LSIModbus RTU and CAM Link600 Vac600 V100% neutral protectionStandard Line and Load25 kAIC at 480 Vac35 kAIC @240V (UL) 10 kAIC Icu @250 Vdc Eaton Power Defense MCCB PDG34FH400P2DJ 3D drawingConsulting application guide - molded case circuit breakers Power Xpert Protection Manager x32Power Xpert Protection Manager x64StrandAble terminals product aidPower Defense technical selling bookletPower Defense molded case circuit breaker selection poster Power Defense brochureMolded case circuit breakers catalogAmperage RatingCircuit breaker frame type FrameNumber of poles Circuit breaker type ClassTrip Type CommunicationVoltage ratingVoltage rating - maxProtectionTerminalsInterrupt rating Interrupt rating range 3D CAD drawing package Application notesBrochuresCatalogsPower Xpert Release trip units for Power Defense molded case circuit breakersCertification reportsPDG3B 450A-600A CB reportPDG3 UL authorization 250-600a TMTUPDG3 CSA certification 100-400aPDG3 UL authorization 250-600a PXRPDG3 45-400A CB reportPDG3 CSA certification 250-600aPDG3 UL authorization 100-400aEU Declaration of Conformity - Power Defense molded case circuit breakersInstallation instructionsPower Defense Frame 3 interphase barrier - IL012229EN H03 Power Defense Frame 1-2-3-4 IP door barrier assembly instructions - IL012278ENPower Defense Frame 3 plug-in adapter installation instructions -IL012311ENPower Defense Frame 3 screw terminal_end cap kit, 400A, 4 pole – IL012263ENPower Defense Frame 3 finger protection assembly installation instructions - IL012279ENPower Defense Frame 3 multi-tap terminal kit Cat NumPDG3X3(2)(4)TA6006W Instructions - IL012248ENPower Defense Frame 3 Breaker Instructions (IL012107EN).pdf Power Defense Frame 2/3/4/5/6 voltage neutral sensor module wiring instructions – IL012316ENPower Defense Frame 3 terminal spreader assembly instructions -IL012301ENPower Defense Frame 3 reverse feed connector kit Cat NumPDG3X3(2)(4)TA630RF instructions - IL012253ENPower Defense Frame 3 box terminal installation instructions -IL012299ENPower Defense Frame 4 locking devices and handle block instructions - IL012151ENPower Defense Frame 3 terminal kit Cat Num PDG3X3(2)(4)TA400RF instructions - IL012251ENPower Defense Frame 3 interphase barriers 3-pole - IL012229EN H01 Power Defense Frame 4 reverse feed connector kit instructions for PDG4X3(2)(4)TA800RF instructions - IL012254ENPower Defense Frame 3 extendable shaft rotary handle mech -IL012112ENPower Defense Frame 3 adapter kit installation instructions LZM3 to PD3 - IL012227ENPower Defense Frame 3 multi wire connector kit -PDG3X3(2)(4)TA4006W and PDG3X3(2)(4)TA4003W instructions-IL012247EN H01Power Defense Frame 3 Aux, Alarm, ST and UVR Animated Instructions.rh Power Defense Frame 3 rear connection installation instructions -IL012300ENPower Defense Frame 3 Direct Rotary Handle Assy With Interlock Version Instructions (IL012139EN).pdfPower Defense Frame 4 shunt trip UVR instructions - IL012129EN Power Defense Frame 3 reverse feed connector kit Cat NumPDG3X3(2)(4)TA400HRF instructions - IL012252ENPower Defense Frame 3 screw terminal end cap kit 600A, 4 pole -IL012265ENPower Defense Frame 3 terminal cover assembly instructions -IL012281ENPower Defense Frame 3 handle mech direct rotary handle instructions - IL012111ENPower Defense Frame 3 locking devices and handle block instructions - IL012150ENPower Defense Frame 3 shunt trip UVR instructions - IL012140EN Power Defense Frame 3 trip unit replacement instructions - IL012157EN Power Defense Frame 3 multi-tap terminal kit Cat NumPDG3X3(2)(4)TA6006WSW instructions - IL012250ENInstallation videosPower Defense Frame 3 trip unit replacement animated instructions.rh Power Defense Frame 3 Shunt Trip_UVR Animated Instructions.pdf.rh Power Defense Frame 3 Locking Devices and Handle Block Animated Instructions.pdf.rhPower Defense Frame 3 Handle Mech Direct Rotary Handle Animated Instructions.rhMultimediaPower Defense Frame 3 Aux, Alarm, Shunt Trip, and UVR How-To Video Power Defense Frame 3 Trip Unit Installation How-To VideoPower Defense Frame 3 Direct Rotary Handle Mechanism Installation How-To VideoPower Defense molded case circuit breakersPower Defense Frame 2 Variable Depth Rotary Handle Mechanism Installation How-To VideoPower Defense Frame 5 Trip Unit How-To VideoPower Defense Frame 6 Trip Unit How-To VideoEaton Corporation plc Eaton House30 Pembroke Road Dublin 4, Ireland © 2023 Eaton. All Rights Reserved. Eaton is a registered trademark.All other trademarks areproperty of their respectiveowners./socialmediaPower Defense Frame 3 Variable Depth Rotary Handle Mechanism Installation How-To Video Power Defense BreakersEaton Power Defense for superior arc flash safety Eaton Specification Sheet - PDG34FH400P2DJ Power Defense time current curve Frame 3 - PD3Intelligent circuit protection yields space savings Making a better machineMolded case and low-voltage power circuit breaker healthImplementation of arc flash mitigating solutions at industrial manufacturing facilitiesIntelligent power starts with accurate, actionable data Single and double break MCCB performance revisited Molded case and low-voltage breaker health Safer by design: arc energy reduction techniquesSpecifications and datasheetsTime/current curvesWhite papers。

Custom AffinityScript Multiple Temperature Reverse

Custom AffinityScript Multiple Temperature Reverse

Custom AffinityScript Multiple Temperature Reverse Transcriptase, PartNumber 930107CHEMTREC®: 4001-204937化学品安全技术说明书GHS化学品标识应急咨询电话(带值班时间)::供应商/ 制造商:Agilent Technologies Shanghai Co., Ltd.LC-China412 Ying Lun RoadWaigaoqiao Free Trade Zone Shanghai 200131 P. R. China 电话号码: 800-820-3278Custom AffinityScript Multiple Temperature Reverse Transcriptase, Part Number 930107化学品的推荐用途和限制用途600105-51600100-52产品号:物质用途:10 反应1 ml(毫升)产品号 (Chemical Kit):930107安全技术说明书根据 GB/ T 16483-2008 和 GB/ T 17519-2013危险性类别含有未知吸入毒性成分的混合物百分比: 30 -60%10X AffinityScript RT Buffer含有未知经皮毒性成分的混合物百分比: 10 -30%含有未知吸入毒性成分的混合物百分比: 10 -30%含有未知经口毒性成分的混合物百分比: 1 -10%混合物中由对水生环境毒性未知的组分组成的比率: 7.9%物质或混合物的分类根据 GB13690-2009 和 GB30000-2013皮肤腐蚀/刺激 - 类别 3H320严重眼损伤/眼刺激 - 类别 2B 10X AffinityScript RT Buffer H316皮肤腐蚀/刺激 - 类别 3H319严重眼损伤/眼刺激 - 类别 2A紧急情况概述液体。

IQFISH Probes 化学品安全技术说明书

IQFISH Probes 化学品安全技术说明书

IQFISH Probes *************(24小时)化学品安全技术说明书GHS product identifier 应急咨询电话(带值班时间)::供应商/ 制造商:安捷伦科技贸易(上海)有限公司中国(上海)外高桥自由贸易试验区英伦路412号(邮编:200131)电话号码: 800-820-3278传真号码: 0086 (21) 5048 2818IQFISH Probes化学品的推荐用途和限制用途G111600-8, G111601-8, G111602-8, G111603-8, G211600-8, G211601-8,G211602-8, G211603-8部件号:物质用途:仅限体外诊断使用0.2 ml(毫升) IQFISH Probes G111600-85510, G111601-85510,G111602-85510, G111603-85510安全技术说明书根据 GB/ T 16483-2008 和 GB/ T 17519-2013GHS化学品标识:IQFISH 探针有关环境保护措施,请参阅第 12 节。

物质或混合物的分类根据 GB13690-2009 和 GB30000-2013紧急情况概述液体。

无资料。

无资料。

H319 - 造成严重眼刺激。

H335 - 可能造成呼吸道刺激。

H373 - 长期或反复接触可能损害器官。

(肾)物理状态:颜色:气味:GHS危险性类别警示词:警告:标签要素混合物中由对水生环境毒性未知的组分组成的比率: 20%象形图急性毒性 (口服) - 类别 5皮肤腐蚀/刺激 - 类别 2H319严重眼损伤/眼刺激 - 类别 2AH335特异性靶器官毒性 一次接触 (呼吸道刺激) - 类别 3H373特异性靶器官毒性 反复接触 - 类别 2H319 - 造成严重眼刺激。

H335 - 可能造成呼吸道刺激。

H373 - 长期或反复接触可能损害器官。

(肾)防范说明预防措施:P260 - 避免吸入蒸气。

碧云天EMSAGel-Shift结合缓冲液说明书

碧云天EMSAGel-Shift结合缓冲液说明书

碧云天生物技术/Beyotime Biotechnology 订货热线:400-168-3301或800-8283301 订货e-mail :******************技术咨询:*****************网址:碧云天网站 微信公众号EMSA/Gel-Shift 结合缓冲液(5X)产品编号 产品名称包装 GS005EMSA/Gel-Shift 结合缓冲液(5X)100次产品简介:EMSA/Gel-Shift 结合缓冲液(5X) (EMSA/Gel-Shift Binding Buffer, 5X)是用于EMSA/Gel-Shift 的结合缓冲液。

通过EMSA ,也称gel-shift ,可以研究目的蛋白和特定的DNA 序列的结合情况,从而可以研究细胞内一些转录因子的激活水平。

EMSA/Gel-Shift 结合缓冲液(5X)可以用于EMSA 研究时细胞核蛋白或纯化的转录因子和特定的双链寡核苷酸的结合反应。

EMSA/Gel-Shift 结合缓冲液(5X)中含有poly(dI-dC),DTT ,甘油,EDTA ,氯化钠,氯化镁以及Tris 等有效成分。

其中poly(dI-dC)的浓度经过优化,可以很好的消除蛋白和标记探针间的非特异性结合,同时又不会减弱目的转录因子和标记探针间的结合。

本EMSA/Gel-Shift 结合缓冲液(5X)不仅适用于同位素标记的探针,也适用于地高辛(digoxin)或生物素(biotin)标记的探针。

每个包装的EMSA/Gel-Shift 结合缓冲液(5X),足够进行100个蛋白和探针的结合反应。

包装清单:产品编号 产品名称包装 GS005 EMSA/Gel-Shift 结合缓冲液(5X)200μl —说明书1份保存条件:-20ºC 保存,一年有效。

注意事项:需自备EMSA 相关的所有其它试剂。

请仔细阅读完整个说明书后,再开始实验操作。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

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