A Study of Changes in Uterine Leucocytes During Early Pregnancy in the Mouse-vole Interspesific

胡黄连苷Ⅱ对非小细胞肺癌恶性进展的影响

胡黄连苷Ⅱ对非小细胞肺癌恶性进展的影响Δ郭寰宇 1＊，王卫芳 2 #，徐丽伟 3，董文博 4（1.长春中医药大学临床医学院实验中心，长春　130117；2.长春中医药大学临床医学院生物化学教研室，长春　130117；3.长春中医药大学附属医院肿瘤血液科，长春　130112； 4.吉林大学第一医院二部检验科，长春　130061）中图分类号 R 965 文献标志码 A 文章编号 1001-0408（2024）04-0430-06DOI 10.6039/j.issn.1001-0408.2024.04.09摘要目的探讨胡黄连苷Ⅱ对非小细胞肺癌（NSCLC ）恶性进展的影响及机制。

方法将A 549细胞分组为对照组，胡黄连苷Ⅱ低、中、高浓度组，K 6PC-5[鞘氨醇激酶1（SPHK 1）激活剂]组，胡黄连苷Ⅱ高剂量+K 6PC-5组，检测细胞增殖、迁移、侵袭情况，以及细胞中增殖细胞核抗原（PCNA ）、基质金属蛋白酶2（MMP-2）、MMP-9、SPHK 1、1-磷酸鞘氨醇受体3（S 1PR 3）及胞外信号调节激酶1/2（ERK 1/2）蛋白的表达情况。

以BALB/c 裸鼠为对象，通过皮下接种A 549细胞悬液建立NSCLC 裸鼠移植瘤模型，并将其分为裸鼠-对照组，裸鼠-胡黄连苷Ⅱ低、中、高剂量组，裸鼠-K 6PC-5组，裸鼠-胡黄连苷Ⅱ高剂量+K 6PC-5组（每组5只），考察胡黄连苷Ⅱ对其瘤体质量及体积的影响。

结果与对照组比较，胡黄连苷Ⅱ低、中、高浓度组的细胞OD 450值、EdU 阳性细胞率、划痕愈合率、细胞侵袭数及PCNA 、MMP-2、MMP-9、SPHK 1、S 1PR 3、ERK 1/2蛋白的相对表达量均显著降低；与裸鼠-对照组比较，裸鼠-胡黄连苷Ⅱ低、中、高剂量组裸鼠体内的瘤体质量及体积均显著降低或缩小，上述指标均呈浓度/剂量依赖性变化（P ＜0.05）；K 6PC-5组细胞和裸鼠-K 6PC-5组裸鼠对应指标的变化趋势则相反（P ＜0.05）。

非小细胞肺癌间质上皮细胞转化因子14外显子跳跃突变的研究进展

非小细胞肺癌间质上皮细胞转化因子14外显子跳跃突变的研
究进展
刘艳萍;罗敏
【期刊名称】《临床医药实践》
【年(卷),期】2024(33)1
【摘要】近年来,随着肿瘤分子生物学研究的不断深入,以表皮生长因子受体为首的分子靶向治疗开启了晚期非小细胞肺癌(NSCLC)治疗的新篇章,患者总生存期(OS)得到显著延长。

间质上皮细胞转化因子(MET)14外显子跳跃突变作为非小细胞肺癌的潜在驱动基因引起人们的关注,MET 14外显子跳跃突变是NSCLC患者治疗的新靶点之一^([1])。

目前已有多种MET-络氨酸激酶抑制剂(TKIs)正在进行临床研究,包括克唑替尼、卡马替尼、替泊替尼、赛沃替尼和谷美替尼等。

本文讨论非小细胞肺癌的MET 14外显子跳跃突变的分子生物学、治疗方法及其发展中遇到的各种挑战。

【总页数】5页(P61-65)
【作者】刘艳萍;罗敏
【作者单位】南宁市第二人民医院
【正文语种】中文
【中图分类】R73
【相关文献】
1.MET14外显子跳跃突变在非小细胞肺癌中的研究进展
2.间质上皮细胞转化因子扩增非小细胞肺癌患者的临床病理特征与预后分析
3.表皮生长因子受体20外显子插入突变的非小细胞肺癌靶向治疗研究进展
4.真实世界中MET14外显子跳跃突变晚期非小细胞肺癌的疗效分析
5.晚期非小细胞肺癌MET 14外显子跳跃突变的治疗新进展
因版权原因，仅展示原文概要，查看原文内容请购买。

科学家用动物做药物研究的英文作文

科学家用动物做药物研究的英文作文Scientists have been conducting research on animals to discover new drugs and treatments for various diseases for many years. This practice, known as animal testing, is a crucial step in the development of pharmaceuticals and medical treatments. While controversial, animal testing has led to numerous medical breakthroughs that have improved human health and saved countless lives.One of the main reasons why scientists use animals in drug research is that they share many biological similarities with humans. This allows researchers to study the effects of new drugs on living organisms in a controlled environment before testing them on humans. Animals such as mice, rats, rabbits, and monkeys are commonly used in these experiments due to their genetic similarities to humans.Animal testing is essential for testing the safety and efficacy of new drugs before they can be approved for use in humans. By observing the effects of a drug on animals, researchers can identify any potential side effects or toxicities that may occur. This helps ensure that only safe and effective drugs are allowed to enter clinical trials and eventually reach the market.Furthermore, animal testing is crucial for understanding the mechanisms of diseases and developing new treatments. By studying how diseases progress in animals and testing new therapies on them, scientists can gain valuable insights into the underlying causes of illnesses and identify potential targets for drug development. This knowledge is essential for finding new cures and treatments for diseases such as cancer, diabetes, and heart disease.Despite its benefits, animal testing is a controversial practice that has sparked ethical concerns among some people. Critics argue that using animals in research is cruel and unnecessary, as there are alternative methods available, such as computer modeling and cell cultures. However, these methods are not always reliable or accurate and cannot completely replace animal testing in drug research.To address ethical concerns, researchers are constantly working to refine and reduce the use of animals in their experiments. They strive to minimize the number of animals used, improve their welfare, and use alternative methods whenever possible. Additionally, scientists are exploring new technologies, such as organ-on-a-chip systems and 3D bioprinting, that may eventually replace animal testing altogether.In conclusion, animal testing is a vital tool in drug research that has played a significant role in advancing medicine and improving human health. While controversial, this practice has led to countless medical breakthroughs and continues to be an essential part of the drug development process. By carefully balancing the need for scientific progress with ethical considerations, researchers can ensure that animal testing is conducted responsibly and humanely.。

灵长类动物卵巢衰老的分子标记物被揭示

灵长类动物卵巢衰老的分子标记物被揭示作者：来源：《科学中国人·下旬刊》2020年第05期灵长类动物卵巢衰老的分子标记物被揭示中国科学院动物研究所刘光慧研究组和曲静研究组与北京大学汤富酬研究组及美国索尔克（Salk）研究所Juan Carlos Izpisua Belmonte研究组等合作，绘制了食蟹猴卵巢的单细胞衰老图谱，同时利用人类卵巢细胞研究体系，发现增龄伴随的抗氧化能力的下降是灵长类卵巢衰老的主要特征之一。

研究论文发表于Cell。

文章报道了非人灵长类器官衰老的高精度單细胞转录组图谱研究，揭示细胞类型特异性的氧化还原调控的失稳是包括人类在内的灵长类卵巢衰老的共性分子机制。

加深了对卵巢组织结构增龄性变化的认识，解析了衰老过程中不同卵巢细胞类型的易感性及易感分子，提供了灵长类卵巢衰老的潜在调控靶标信息。

转基因猕猴与部分自闭症患者的脑功能网络异常相似中国科学院脑科学与智能技术卓越创新中心（神经科学研究所）、上海脑科学与类脑研究中心王征研究组发现转基因猕猴与部分自闭症患者的脑功能网络异常相似。

研究论文发表于Journal of Neuroscience。

该研究联合运用基因组学、行为学、多通道脑电以及功能磁共振成像技术对转基因猕猴系统性分析发现，MECP2基因过表达引起一连串生理事件变化，包括GABA信号通路，β频段脑电信号同步性以及脑功能网络连接异常变化，并进一步展示转基因猕猴的脑功能网络异常与小部分临床自闭症患者的磁共振脑影像结果非常相似，为非人灵长类模型未来的转化应用奠定神经环路基础。

魏氏准噶尔翼龙头骨腭区研究进展中国科学院古脊椎动物与古人类研究所汪筱林团队关于魏氏准噶尔翼龙的头骨腭区研究最新进展发表于PeerJ。

魏氏准噶尔翼龙（Dsungaripterus weii）是我国发现的第一具较完整的翼龙化石骨架，1964年杨钟健研究命名并建立了准噶尔翼龙科，当时认为这是第一次在我国发现的翼龙类化石。

跨物种扩增筛选获得蓝马鸡Crossoptilon auritum微卫星多态分子标记英文

Chinese Birds 2012,3(2):103–107SHORT COMMUNICA TIONReceived 04March 2012;accepted 10May 2012Author for correspondence (Zhengwang Zhang)E-mail:zzw@*Y ang L iuPresent address:State Key L aboratory of Biocontrol and School of LifeSciences,Sun Y at-Sen University,Guangzhou 510275,ChinaA panel of polymorphic microsatellites in the Blue Eared P heasant (Crossoptilon auritum)developed by cross-species amplificationLangyu GU 1,Y ang LIU 2,*,N ngA NG 1,Zhengwang ZHA NG 1,1MOE K ey Laboratory for Biodiv ersity Sciences and Ecological Engineering,College of L ife Sciences,Beijing Normal University ,Beijing 100875,China2Computational and Molecular Population Genetics,Institute of Ecology and Ev olution,Univ ersity of Bern,Baltzerstrasse 6,3012,Bern,SwitzerlandAbstr act Polymorphic microsatellites are among the versatile genetic markers in molecular ecology studies.In contrast to de no vo isolation of microsatellites from target species,cross-species ampli ca-tion is a cost-effective approach for a fast development of microsatellite markers from closely related taxa.In our study ,we cross-ampli ed a panel of poly morphic microsatellite markers for the Blue Eared Pheasant (Crosso ptilon auritum),a species endemic to China of considerable conservation con-cern.We obtained 11polymorphic microsatellite markers selected from 112candidate loci,originally isolated from other Galliforme species.This panel of makers has shown moderate to high lev els of polymorphism and include a Z-chromosomal linkage locus.We carried out preliminary analy ses of parentage among captive individuals with a known pedigree using this new panel of microsatellites.Our results suggest that the high utility of these markers may be powerful tools for studies in conser-vation genetics of eared-pheasants and other endang ered Galliforme species.Keywords Crossoptilon auritum,microsatellites,cross-species ampli cation,Z-chromosomal linkag eInt roductionEndemic species have long been a key focus in conser-vation efforts (Myers et al.,2000),given that the levelof endemics might be positiv ely correlated with species richness (Lamoreux et al.,2006).Besides,endemic spe-cies with limited dispersal capacity might be sensitive to changes in local climate,or vulnerable to invasive spe-cies (Ohlem üller et al.,2008).A good understanding of ev olutionary processes such as population subdivisions,changes of effective population size and genetic connec-tivity of endemic species would shed light on evolution-ary processes as well as on conservation manag ement.The Blue Eared Pheasant (Crossoptilon auritum),belonging to Phasianidae,Galliformes,is a rare and en-demic pheasant species in western China (L ei and L u,2006).Its wild populations are found at Helan Moun-Chinese Birds2012,3(2):103–107 104tain,as well as along the eastern edge of the Qinghai-Tibetan Plateau(QTP),cov ering Qing hai,Gansu and Sichuan provinces(L ei and L u,2006).A lthough previ-ous studies have been carried out on the biology and ecology of C.auritum(Sun et al.,2005;Li et al.,2009; Wu and L iu,2010),a thorough assessment of genetic diversity is urg ently needed to assess the population vi-ability of this species.Furthermore,apart from C.auri-tum,the genus Crosso ptilon includes three other species, i.e.,C.mantchuricum,C.harmani and C.cro ssoptilo n. These species are endemic to China(Zheng,2011)and all are listed on the IUCN Red L ist of Threatened Spe-cies(IUCN2011)because of a rapid decline in the size of their population,caused by habitat fragmentation and hunting(Lei and Lu,2006).Giv en these concerns, obtaining molecular markers is a prerequisite in un-derstanding the genetic background of C.auritum and might be useful for population genetic studies in Cros-so ptilon species.Microsatellites are powerful tools for conservation genetic studies such as population genetics,mating systems and inv estigations into kinship(Primmer et al., 2005;Karl et al.,2011).Compared with isolated novel microsatellite markers,cross-species microsatellite am-pli cation from closely related species is cost-effective (Zane et al.,2002).More importantly,it has been sug-gested that this method has successfully worked among species belonging to the same genera,different genera and even different families(Barbaráet al.,2007;Huang and L iao,2010).Given that numerous microsatellite markers have been dev eloped for various Phasianidae species(Cheng et al.,1995;Wang et al.,2009;Zhou and Zhang,2009),we attempted to establish microsatellite markers for C.auritum through cross-species ampli-cation from a large number of marker candidates.In order to test the effectiveness of these markers,we also carried out preliminar y parentage analysis among cap-tive individuals of known pedigree.Mate ria ls and methodsA total of20C.auritum blood samples from brachial veins were collected to develop microsatellite loci by cross-species ampli cation,nine of which were from the Linxia Zoo in Gansu Province and the other11 from Huzhu in Qinghai Province,China.Additionally, nine individual birds from two families of the Beijing Wildlife Park in Daxing and Beijing Zoo were used to conduct parentage analy sis.We have detailed the known pedig ree of these two families of birds.Genomic DNA was extracted using DNA extraction kits(Tian Gen Biotech,Beijing,China).The cross-spe-cies microsatellite markers(Table1)came from various Galliforme species,including16loci from Meleag risgal-lopavo(Burt et al.,2003),30from T rag opan temminck ii (Zhou and Zhang,2009),20from Syrmatic us reevesii (Wang et al.,2009),41from Gallus gallus(Cheng et al., 1995;Dawson et al.,2010),four from C.mantchuric um (Zhao et al.,Beijing Normal Univ ersity,unpublished results)and one from Syrmaticus mikado(S.H.L i,T ai-wan Normal Univ ersity,unpublished results).After ltering out those loci with poor cross-ampli cation, the polymorphism of the remaining pairs were tested with either6-FA M or HEX uorescent dyelabeled on 5′of a single forward primer.Polymerase chain reac-tion(PCR)was carried out in a10L reaction system containing100ng DNA,0.25L of each primer,1L of a10×PCR buffer,1.5mM Mg Cl2,0.2mM dNTP mix and0.75U T aq polymerase(Takara,Japan).The reac-tion was denatured at94°C for5min,followed by40 cy cles at94°C for30s,a touch-down annealing process from58–47°C,reducing in steps of0.5°C per cycle and another20cycles annealing at47°C and then72°C for 50s,with a nal extension at72°C for5min.Fragment analy sis was conducted on an ABI PRISM3100Genetic Analyzer using the GeneMapper software(Applied Biosystems)with ROX-500as the standard for size.We conducted PCR ampli cation and fragment analysis at least twice to ensure the accuracy of individual geno-types.Sequencing of selected homozygotes was also conducted in both directions with ampli cation prim-ers(BGI Bio T ech,Beijing,China)to ensure that the products were genuine microsatellites.We blast the acquired microsatellite sequences from C.auritum on the chicken genome in GenBank to nd their locations and to inv estigate the potential linkages among the loci.If some loci were mapped in sex chro-mosome,we carried out sex ing identi cation with ourLangyu Gu et al.Microsatellite DNA loci in Blue Eared Pheasant105sample set by using primer sex1/sex2(Wang and Zhang,2009)to cross-validate the sex-linkage loci.We tested observed heterozygosity(HO),expectedheterozygosity(HE),the number of allele per locus, the Hardy-Weinberg equilibrium(HWE)and linkage-disequilibrium in each population using A rlequinv.3.11 (Excof er et al.,2005).W e calculated the frequencies of null alleles by using FreeNA(Chapuis and Estoup, 2007).High polymorphic markers with HWE were used for paternity tests in CERVUS3.0with100000 times simulations to estimate their resolving power. Signi cant levels were recorded after application of the sequential Bonferroni correction(Rice,1989;Ex cof er et al.,2005).ResultsSixty-two(55.4%)of the112cross-species markers could be ef ciently ampli ed in C.auritum,while11 (17.7%)of the62markers had a moderate to high level of polymorphism(3–11loci),with the expected het-erozyg osities ranging from0.42to0.89(T able1).Locus 4C12was found to be homozyg ous in heterogametic fe-males and heterozy gous in56.2%of the males(n=28) and thus most likely to link with the Z chromosome. Three loci(1H4,2420and4F8)were not targ eted on the chicken genome.A ll loci were at the HWE;neither linkage disequilibrium among pairs of loci nor null al-leles was found.The parentage analysis results showedTable1Characterization of11microsatellite lociL ocus Primer sequences(5′–3′)A ccessionNo.Repeatmotifsn NASize range(bp)HOHESpecies origin Chromosome2580F:TTAACCTA TCAGGTCGTTGCG AL592580(CA)n208191–213 1.000.80M.gallopavo21 R:CA GTGCACA TGCA GGCA G3D2F:TCTCTGA CGTA TCGCA TCT FJ221373(GT)n196286–3040.470.58S.reevesii4 R:A CTTCCCCTGGTAAA CT1H4F:TGAACA AGTGA GGCGGAGC/(TG)n2010127–1610.650.81S.reevesii/ R:CTGCACA CAGCCCGAA GC2420F:CA TCA TCTGCCAA TGCA GAGG/(TTTA)n204118–1420.550.54S.mikado/ R:A AGCCCA TA TA TGCTTCCTGG4H1F:TA TGAAA CAGA CTTAA TCC FJ221388(GTTT)n204203–2110.850.67S.reevesii1 R:TGCAGCA TTTGA GT AAC5C9F:TA TGGGAA A TGTGTACCTTT A GQ184557(CA)n2010221–2590.950.89 C.mantchuricum10 R:TCCA GGCAA CACGTAA CATT06F:TGAGAGA TTTTGACCCA GQ181183(CA)n207225–2370.850.83T.temminckii6 R:CAA GACTTCA CCCTA CAGA TA4F8F:GTGGCA TGCCTAGTA GA TGTT/(A C)n2011186–2140.750.88 C.mantchuricum/ R:CCCTGTGGTA CGAACTGTCSR11F:A TCAA T A TGGACTGCTCCGT FJ221381(TG)n205210–2480.550.58S.reevesii17 R:TCCTTCA AGGCCAAGTG5H7F:CCAA GAGGGA GGCA CACGTTC U60782(TG)n203186–1940.550.42G.gallus8 R:A GCCA TAAA T AAGCAAA CGC4C12F:A T AGGCGGACA GAGGA T AGA FJ221385(CA)n204160–1700.300.59S.reevesii Z R:CCCCGCA TCGAGGTGNotes:n is the number of successfully genotyped individuals and NA the number of alleles.HOis observed heterozygosity and HEexpected heterozygosity.Chinese Birds2012,3(2):103–107 106that if neither parent was known,the successful assig n-ment rates were98%at a95%con dence lev el and 98%at an80%con dence level.Paternity test results are consistent with known pedigrees.DiscussionThe phylogenetic relationship between the original and target species seems to affect the success of cross-species ampli cation(Primmer et al.,1996).In our analyses, cross-species ampli cation from Crossoptilon was the most effective(2/4=50%),followed by those from Syrmaticus(6/21=28.6%),Meleag ris(1/16=6.25%), Trag opan(1/30= 3.0%)and Ga llus(1/41=2.4%). Phylogenetic analysis showed a successiv e relationship between Crossoptilon and the genera mentioned earlier (Kimball et al.,2011),indicating that evolutionary re-lationships may play an important role in cross-species ampli cation of microsatellite markers within Gallifor-mes.A lthough large numbers of microsatellite markers are found in autosomes,Z-linked microsatellites markers are still rarely av ailable,ev en in the well-characterized chicken genome(Groenen et al.,2000).One Z-linked marker TUT,originally isolated from the T etrao uro gal-lus(Seg elbacher et al.,2000,Wang et al.,2011),failed to be ampli ed in chickens and mig ht be speci c for T etr-aoninae grouse.In the present study,the Z-linked poly-morphic locus4C12seems to hav e general application in different Galliforme species and might yet provide a valuable tool in kinship and demographic analyses combined with other loci.Acknowledgements The study was supported by the National Key Project of the Scienti c and Technical Supporting Programs Funded by the Ministry of Science and T echnology of China (2012BA C01B06)and the National Natural Science Founda-tion of China(No.30570234).We thank Jiliang X u,Xinli Zhao, Jiang Chang and Ying Liu for the collection of samples,as well as administrations of the Y inchuan Zoo in Ningxia A utonomous Region,the Xining Zoo in Qinghai Province,the Linxia Dongjiao Zoo in Gansu Province and the Beijing Wildlife Park and Beijing Zoo for providing samples.We thank Xinli Zhao for provid-ing unpublished markers from Crossoptilon mantchuricum and Shou-Hsien Li for providing unpublished markers from Syrmati-cus mikado.We thank Lu Dong,Xiangjiang Zhan,Ning Wang and Y ingying Liu for guidance with data analysis.ReferencesBarbaráT,Palma-Silva C,Paggi GM,Bered F,Fay MF,L exer C.2007.Cross-species transfer of nuclear microsatellite markers: potential and limitations.Mol Ecol,16:3759–3767.Burt DW,Morrice DR,Sewalem A,Smith J,Paton IR,Smith EJ, Bentley J,Hocking PM.2003.Preliminary linkage map of the T urkey(Meleagris gallopavo)based on microsatellite markers.A nim Genet,34:399–409.Chapuis MP,Estoup A.2007.Microsatellite null alleles and esti-mation of population differentiation.Mol Biol Evol,24:621–631.Cheng HH,Levin I,V allejo RL,Khatib H,Dodgson JB,Critten-den LB,Hillel J.1995.Development of a genetic map of the chicken with markers of high utility.Poult Sci,74:1855–1874. 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蛞蝓提取物对肺癌细胞周期的影响

３８
内蒙古中医药
蛞蝓提取物对肺癌细胞周期的影响△
韦金育曾振东曾翠琼俸曙光杨增艳摘要：目的：了解蛞蝓提取物对肺癌细胞周期的影响。法：流式细胞仪分析蛞蝓提取物对Ａ４Ｎ胞周期的影响。结果：方用５９蛞蝓提取物能使细胞的Ｓ（Ｎ合成期）降。结论：蝓提取物可能通过诱导Ａ４Ｎ胞周期阻滞从而发挥抗癌作用。期ＤＡ下蛞５９
中图分类号：６３Ｒ８．２
１材料与方法
文献标识码：Ｂ
文章编号：０６０７（０２— ０９０１０— ９９２１）４０３— ２１
神经与椎体外缘的夹角。１标本制备：．１尽量不损伤神经血管，暴露ＴＬ的椎体、弓根、２２１椎．左右两侧椎间孔外口处神经血管的排列走行是：自上而下为椎间孑、神经、管等结构。Ｌ脊血椎间静脉、神经、动脉即节段动脉的中间支（１；脊根图）出椎间孔
２１年１０１２月
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其抑制作用主要是通过抑制细胞内ＤＡ的合成而实现的。本实嘲．Ｎ中国中西医结合杂志，９，（）２．１９１９４３９９：验结果也许提示这可能是蛞蝓抗肿瘤作用的机理之一。［甄永苏．瘤药物研究与开发『］３】抗肿Ｍ．北京：工出版社．０．１化２４１．００
蛞蝓提取物对人肺癌的作用良好、毒性较小、资源丰富，得【郭岳峰，守章，民，．蝓胶囊治疗晚期非小细胞肺癌３值４］王焦智等蛞２进一步研究。例疗效观察中医研无１９，３：．９４７）４（２参考文献［］金魁漕建国．蝓对人肺鳞癌、５谢蛞肺腺癌细胞抑瘤作用初探『．Ｊ１［李仪奎．１］中药药理实验方法学【．１．海：海科学技术出Ｍ】第版上上肿瘤防治研究，９，（）４．１７４６：４９２３

《2024年旋毛虫致人小细胞肺癌H446细胞凋亡的研究》范文

《旋毛虫致人小细胞肺癌H446细胞凋亡的研究》篇一一、引言旋毛虫作为一种罕见的寄生虫，对人体的影响具有极大的危害性。

近期研究发现，其不仅对人体寄生虫病产生影响，甚至可能与肺癌的发病有某种联系。

尤其对于小细胞肺癌H446细胞，旋毛虫的存在可能对其产生凋亡作用。

本文旨在探讨旋毛虫对H446细胞凋亡的影响及其潜在机制。

二、材料与方法1. 材料本实验采用小细胞肺癌H446细胞株作为研究对象，旋毛虫的提取液作为实验处理因素。

所有试剂均经过严格筛选和验证，确保实验的准确性。

2. 方法（1）细胞培养：H446细胞在适宜的培养条件下进行培养，并定期观察其生长状态。

（2）旋毛虫提取液制备：从旋毛虫中提取有效成分，制备成实验所需的旋毛虫提取液。

（3）实验分组：将H446细胞分为实验组和对照组，实验组加入不同浓度的旋毛虫提取液进行处理。

（4）细胞凋亡检测：通过流式细胞术、Western blot等方法检测H446细胞的凋亡情况。

三、实验结果1. 旋毛虫提取液对H446细胞的生长影响实验结果显示，随着旋毛虫提取液浓度的增加，H446细胞的生长受到明显抑制。

高浓度的旋毛虫提取液甚至导致细胞死亡。

2. 旋毛虫提取液诱导H446细胞凋亡流式细胞术和Western blot结果显示，旋毛虫提取液能够诱导H446细胞发生凋亡。

随着浓度的增加，凋亡的细胞比例逐渐增加。

同时，凋亡相关蛋白的表达也发生了明显变化。

3. 凋亡机制探讨通过进一步的研究，我们发现旋毛虫提取液诱导H446细胞凋亡的机制可能与线粒体途径有关。

旋毛虫提取液能够引起线粒体膜电位下降，释放细胞色素C等凋亡相关因子，从而触发细胞凋亡。

四、讨论本研究发现，旋毛虫提取液能够抑制H446细胞的生长并诱导其发生凋亡。

这一现象可能与线粒体途径有关。

此外，我们还发现不同浓度的旋毛虫提取液对H446细胞的凋亡影响不同，这提示我们在实际治疗中需要控制旋毛虫提取液的浓度以避免过度损伤正常细胞。

洛铂联合紫杉醇治疗子宫内膜癌的效果及毒副反应观察

洛铂联合紫杉醇治疗子宫内膜癌的效果及毒副反应观察任妞，张晓莉，金伟丽,刘志丹(中国人民解放军联勤保障部队第九八九医院妇产科，河南洛阳471003)揖摘要】目的探究紫杉醇联合洛铂治疗子宫内膜癌的效果及毒副反应观察。

方法选择2015年1月至2018年1月就诊的子宫内膜癌患者120例，随机分成观察组和对照组，各60例。

对照组采用顺铂+紫杉醇进行静脉化疗，观察组采用洛铂+紫杉醇进行静脉化疗。

比较两组治疗后临床疗效、治疗期间毒副反应，并随访2年，观察两组生存情况。

结果治疗后，观察组总有效率为58.33%,对照组为53.33%,两组差异无统计学意义(P>0.05)；治疗期间，两组白细胞下降、血小板减少、周围神经炎的发生率无明显差异(P>0.05),观察组恶心呕吐、肝损害、肾损害发生率低于对照组(P<0.05)；随访2年，观察组的2年总生存率为76.67%袁对照组为71.67%,差异无统计学意义(P>0.05)遥结论洛铂联合紫杉醇与顺铂联合紫杉醇治疗子宫内膜癌均具有较好疗效,能改善患者生存情况,但洛铂联合紫杉醇的毒副反应较轻。

揖关键词】紫杉醇;洛铂;子宫内膜癌;毒副反应中图分类号：R711.74文献标志码：A DOI：10.3969/j.issn.1003-1383.2021.03.009Observation on efficacy and adverse reactions of lobaplatin combinedwith paclitaxel in the treatment of endometrial cancerREN Niu,ZHANG Xiaoli,JIN Weili,LIU Zhidan(Department of Obstetrics and Gynecology,No.989Hospital of Joint-Service SupportForce of Chinese People's Liberation Army,Luoyang471003,Henan,China)揖Abstract]Objective To explore the efficacy and adverse reactions of lobaplatin combined with paclitaxel in the treatment of endometrial cancer.Methods A total of120endometrial cancer patients who were treated in hospital from January 2015to January2018were enrolled and randomly divided into observation group and control group，with60cases in each group.The control group were given intravenous chemotherapy with cisplatin+paclitaxel，and the observation group were given intravenous chemotherapy with lobaplatin+paclitaxel.And then，the clinical efficacy and side effects were compared between the two groups，and the patients were followed up for2years to observe the survival of them.Results After treat-ment，the total effective rate was58.33%in the observation group and53.33%in the control group，difference was not statistically significant(P>0.05).During treatment，there was no statistically significant difference in the incidence of leucocyte decline，thrombocytopenia and peripheral neuritis between the two groups(P>0.05).The incidence of nausea and vomiting，liver damage，and kidney damage in the observation group was lower than that in the control group(P<0.05).After2years of follow-up，2-year overall survival rate was76.67%in the observation group and71.67%in the control group，difference was not statistically significant(P>0.05).Conclusion Both lobaplatin combined with paclitaxel and cisplatin combined with paclitaxel have good curative effects in the treatment of endometrial cancer，and they can improve the survival of patients.However，side reactions of lobaplatin combined with paclitaxel are relatively milder.揖Key words]paclitaxel；lobaplatin；endometrial cancer；curative effect；side reaction子宫内膜癌是一种常见女性恶性肿瘤，临床多采用手术治疗，早期患者的生存率较高，但是晚期及作者简介:任妞，女,主治医师,医学硕士，研究方向:妇科肿瘤。

中国科学家对细胞遗传学研究的贡献

英文回答：Our scientists have made major breakthroughs in the field of cytogenetics， revealing important patterns of cytogenetics through research into basic biological processes such as cell division， DNA reproduction and transfer of genetic material。

They discovered key proteins associated with cell fragmentation，clarified molecular mechanisms of cell fragmentation and provided an important theoretical basis for the treatment of diseases such as cancer。

Our scientists have also made significant achievements in the regulation of the transfer and expression of genetic material， untying the relationship between genomic stability and genetic diseases and providing important leads for the diagnosis and treatment of related diseases。

They have provided important support for the development of areas such as cell therapy and regenerative medicine in front—line research in the field of cytogenetics，such as cell signaling and stem cell biology。

益经汤对环磷酰胺所致卵巢早衰小鼠的治疗作用研究

广东药科大学学报Journal of Guangdong Pharmaceutical University Jan, 2024, 40(1)益经汤对环磷酰胺所致卵巢早衰小鼠的治疗作用研究牛逸琳1，陈烁1，陈雨诗1，何冬梅2，尹永芹1（1.广东药科大学，广东广州 510006； 2.惠州市中医医院，广东惠州 516001）摘要：目的探究中药复方益经汤对环磷酰胺（CTX）所致卵巢早衰（POF）小鼠的治疗作用。

方法腹腔注射CTX 建立POF小鼠模型，随机分为模型组、阳性对照组、益经汤低、高剂量组和空白组，每组6只，灌胃给药 30 d。

检测小鼠动情周期、体质量，卵巢、子宫指数和组织形态变化；ELISA检测血清中雌二醇（E）、促卵泡刺激素（FSH）、促黄体2生成素（LH）、抗缪勒氏管激素（AMH）、孕酮（PROG）的浓度；免疫组织化学法（IHC）检测卵巢PI3K、AKT、FOXO3A 蛋白表达水平。

结果益经汤干预模型小鼠后，动情周期趋向正常，高剂量组体质量、卵巢指数、子宫指数皆显著升高（P<0.05）；卵巢体积增大，初级和次级卵泡数量增多，细胞纤维化改善；子宫内膜增厚，恢复子宫腺上皮细胞排列；阳性对照组和益经汤高剂量组血清E、AMH、PROG均升高（P<0.05，P<0.01），FSH、LH显著下降（P<0.01）。

2益经汤组和阳性对照组PI3K、AKT、FOXO3A蛋白表达皆显著降低（P<0.01）。

结论益经汤可有效改善动情周期，、AMH、PROG和降低FSH、LH的表达，机制可能与下调PI3K、AKT和恢复卵巢和子宫的功能，可提高血清E2FOXO3A 蛋白表达有关。

关键词：益经汤；卵巢功能；动情周期；性激素中图分类号： R285.5 文献标识码： A 文章编号： 2096-3653（2024）01-0001-07DOI: 10.16809/ki.2096-3653.2023091402Study on the therapeutic effect of compound Yijing decoction on cyclophosphamide-induced premature ovarian failure in miceNIU Yilin1, CHEN Shuo1, CHEN Yushi1, HE Dongmei2*, YIN Yongqin1*(1.Guangdong Pharmaceutical University, Guangzhou 510006, China; 2.Huizhou Hospital of Traditional Chinese Medicine, Huizhou 516001, China)*Corresponding author HEDongmei,Email:***************;YINYongqin,Email:****************** Abstract: Objective To explore the therapeutic effect of traditional Chinese medicine compound Yijing decoction on cyclophosphamide (CTX)-induced premature ovarian failure (POF) in mice. Methods The POF mouse model was established by intraperitoneal injection of CTX, and was randomly divided into the model group, positive control group, low and high doses of Yijing decoction groups, and blank group, with 6 mice in each group. The drug was administered by gavage for 30 d. The estrous cycle, body weight, ovarian and uterine indices, and, FSH, LH, AMH and changes in ovarian and uterine tissues of mice were measured. The concentration of serum E2PROG were detected with ELISA. The levels of ovarian PI3K, AKT, and FOXO3A protein were detected by immunohistochemistry (IHC). Results After intervention with Yijing decoction in mice with POF, the estrus cycle tended to be normal. The body weight, ovarian index, and uterine index significantly increased in the high-dose group mice (P<0.05). The ovarian volume and the number of primary and secondary follicles were increased, and the phenomenon of interstitial and fibrosis was improved. The endometrium was significantly thickened, and the arrangement of endometrial glandular epithelial cells was restored. The levels of serum E, AMH and PROG were2increased in both the positive control group and the high-dose Yijing decoction group (P<0.05, P<0.01), while levels of FSH and LH were significantly decreased (P<0.01). Meantime, the expression of PI3K, AKT and收稿日期：2023-09-14基金项目：广东省惠州市中医医院博士工作站科研项目（BSGZZ03）；广东省中医药局科研项目（20242050）作者简介：牛逸琳（1998－），女，硕士研究生，研究方向为中药学，Email：**********************通信作者：何冬梅（1978－），女，博士，主治中医师，主要从事妇产科临床工作，Email：***************尹永芹（1977－），女，博士，教授，主要从事中药及天然药物活性成分研究，Email：******************。

这种植物分子能显著延长小鼠寿命！它能成为抗衰老新药吗？

这种植物分子能显著延长小鼠寿命！它能成为抗衰老新药吗？药明康德/报道今年7月，我们报道了一篇来自《自然》子刊《Nature Medicine》上的研究。

在这项研究中，科学家们发现几种常见分子有望显著延长动物的生命，并减缓它们的衰老症状。

而在近日，这支团队在这个基础之上，又做了进一步的研究。

他们发现一种叫做漆黄素（fisetin）的类黄酮分子，可能是一种强效的抗衰老药物。

动物为什么会衰老？这是一个很复杂的问题，但其中的关键之一在于“衰老细胞”。

我们知道随着年龄的增长，细胞会不断累积小毛小病，并逐渐失去正常的功能。

在一般情况下，这些细胞会发生自我凋亡，不给人体添乱。

但有时，它们会失去凋亡能力。

于是，这些“老而不死”的细胞在组织和器官中越积越多，不但不能行使正常功能，还会不断释放导致炎症的分子，影响其他健康细胞。

很明显，如果想要不受衰老的困扰，当务之急就是清除这些衰老细胞，这正是这支研究团队的专注方向。

在今年7月的《Nature Medicine》研究中，他们发现达沙替尼（dasatinib）和槲皮素（quercetin）能特异性地杀死衰老细胞，并延长小鼠生命达36%。

而在本次发表的最新研究里，他们则专注于寻找更多的抗衰老分子。

▲这种结构简单的分子，能成为抗衰老新药吗？（图片来源：By Ayacop [Public domain or Public domain], from Wikimedia Commons）先前我们提到的槲皮素是一种类黄酮分子。

还有其他类黄酮分子有抗衰老的潜力吗？为了回答这个问题，科学家们对一系列类黄酮进行了筛选，并在鼠类和人类的成纤维细胞中评估这些分子的抗衰老效果。

研究发现，一种叫做漆黄素的分子在清除衰老细胞上，有着极佳的效果，这甚至要超过了槲皮素。

▲漆黄素（fisetin）清除衰老细胞的能力，甚至要高于槲皮素（quercetin）（图片来源：参考资料[1]）研究人员们也知道，这毕竟只是细胞实验的结果。

《藻蓝蛋白改善半乳糖致衰小鼠卵巢功能的转录组分析》范文

《藻蓝蛋白改善半乳糖致衰小鼠卵巢功能的转录组分析》篇一一、引言近年来，卵巢功能衰退问题在女性健康领域备受关注。

其中，半乳糖是引起卵巢功能减退的重要原因之一。

而藻蓝蛋白作为一种天然的生物活性成分，被广泛研究并认为具有多种生物活性。

本篇论文旨在通过转录组分析，探讨藻蓝蛋白在改善半乳糖致衰小鼠卵巢功能中的作用机制。

二、材料与方法1. 实验动物及分组本实验采用昆明小鼠，按照半乳糖含量将其分为四组：正常对照组、半乳糖处理组（即衰老组）、半乳糖+藻蓝蛋白低剂量处理组以及半乳糖+藻蓝蛋白高剂量处理组。

2. 实验材料与藻蓝蛋白干预将不同浓度的藻蓝蛋白进行干预，通过连续喂养一定周期后，进行后续实验操作。

3. 卵巢组织取材与转录组测序对各组小鼠的卵巢组织进行取材，提取总RNA，并使用转录组测序技术对RNA样本进行深度测序和分析。

三、结果1. 转录组数据概况经过对各组小鼠卵巢组织的转录组数据进行分析，发现半乳糖处理组中存在大量的基因表达异常。

而加入藻蓝蛋白干预后，基因表达情况得到明显改善。

2. 差异表达基因分析对转录组数据中的差异表达基因进行分析，发现在半乳糖致衰模型中，有数百个基因的表达出现异常。

而在藻蓝蛋白干预后，部分基因表达情况得以恢复。

其中，涉及卵巢功能相关的重要基因如FOXL2、BMP15等表达水平明显上升。

3. 信号通路分析通过分析差异表达基因涉及的信号通路，发现藻蓝蛋白主要通过调控与卵巢功能相关的信号通路如MAPK、PI3K-Akt等途径，发挥其改善卵巢功能的作用。

4. 基因功能注释与富集分析对差异表达基因进行功能注释和富集分析，发现藻蓝蛋白主要在细胞增殖、凋亡、免疫反应等方面发挥重要作用。

同时，还涉及了与卵巢功能相关的激素调节、能量代谢等过程。

四、讨论通过对转录组数据的分析，我们发现藻蓝蛋白在改善半乳糖致衰小鼠卵巢功能方面具有显著作用。

通过调控与卵巢功能相关的信号通路及基因表达，改善了半乳糖导致的卵巢功能减退。

科学家用动物做药物研究的英文作文

科学家用动物做药物研究的英文作文英文回答：The use of animals in biomedical research has been a controversial topic for decades. Animal rights activists argue that using animals for experimentation is cruel and unnecessary, while scientists contend that animal researchis essential for advancing medical knowledge and developing new treatments and cures for human diseases.There are many different types of animal research, but the most common type involves using animals to test new drugs and treatments. Animals are used in these studies because they have similar biological systems to humans, and they can provide valuable information about how new drugs and treatments will affect the human body.Animal research has led to the development of many important medical advances, including vaccines, antibiotics, and cancer treatments. However, the use of animals inresearch also raises ethical concerns. Some people believe that it is wrong to use animals for experimentation, and that animals should be given the same rights as humans.The debate over animal research is likely to continue for many years to come. However, it is important to remember that animal research has played a vital role in the development of many important medical advances, and that it is essential for continuing to improve our understanding of human health and disease.中文回答：动物在药物研究中的应用。

《2024年旋毛虫致人小细胞肺癌H446细胞凋亡的研究》范文

《旋毛虫致人小细胞肺癌H446细胞凋亡的研究》篇一一、引言肺癌是全球范围内最常见的恶性肿瘤之一，其中小细胞肺癌（SCLC）是肺癌的一种亚型，具有高度侵袭性和低生存率。

近年来，随着环境和生活方式的改变，肺癌的发病率和死亡率持续上升，因此研究其发生、发展机制和治疗方法变得尤为重要。

本篇研究着重于旋毛虫感染与小细胞肺癌H446细胞凋亡之间的关系，试图通过深入探讨其机制，为肺癌的治疗提供新的思路和方法。

二、研究背景旋毛虫是一种寄生虫，其感染与多种疾病的发生、发展有关。

近年来，有研究表明旋毛虫的某些成分可能具有抗肿瘤活性。

然而，旋毛虫感染是否会直接导致人小细胞肺癌H446细胞的凋亡，以及其具体机制尚不清楚。

因此，本篇研究旨在探讨旋毛虫与小细胞肺癌H446细胞凋亡之间的关系。

三、材料与方法本研究使用小细胞肺癌H446细胞作为实验材料，将旋毛虫的不同组分作用于细胞，观察其对细胞凋亡的影响。

具体方法如下：1. 实验材料准备：采集小细胞肺癌H446细胞及旋毛虫的不同组分。

2. 实验分组：将细胞分为对照组和实验组，实验组又根据旋毛虫不同组分的处理分为若干小组。

3. 实验操作：将旋毛虫的不同组分作用于H446细胞，观察细胞的生长状态、凋亡情况等指标。

4. 数据处理：采用流式细胞术、Western Blot等方法检测细胞的凋亡情况及相关蛋白表达水平。

四、实验结果1. 旋毛虫对H446细胞生长的影响：与对照组相比，实验组中经旋毛虫处理的H446细胞生长受到明显抑制。

2. 细胞凋亡情况：流式细胞术检测结果显示，经旋毛虫处理的H446细胞凋亡率明显升高。

3. 相关蛋白表达水平：Western Blot结果显示，与对照组相比，实验组中凋亡相关蛋白的表达水平明显升高。

五、讨论本研究结果表明，旋毛虫的某些成分可能具有促进小细胞肺癌H446细胞凋亡的作用。

这可能与旋毛虫的某些生物活性成分有关，这些成分可能通过影响细胞的生长、增殖、凋亡等过程，从而达到抗肿瘤的效果。

《2024年旋毛虫虫体蛋白诱导小细胞肺癌H446细胞凋亡机制的研究》范文

《旋毛虫虫体蛋白诱导小细胞肺癌H446细胞凋亡机制的研究》篇一一、引言肺癌是全球范围内最常见的恶性肿瘤之一，其中小细胞肺癌（SCLC）是肺癌的一种亚型，具有较高的侵袭性和转移性。

目前，针对小细胞肺癌的治疗手段有限，因此，寻找新的治疗策略和药物靶点显得尤为重要。

旋毛虫是一种寄生虫，其虫体蛋白具有一定的生物学活性。

近年来研究发现，旋毛虫虫体蛋白可能具有抗肿瘤活性。

本文旨在研究旋毛虫虫体蛋白对小细胞肺癌H446细胞的凋亡机制，以期为肺癌的治疗提供新的思路和靶点。

二、材料与方法1. 材料（1）旋毛虫虫体蛋白：从旋毛虫中提取纯化得到。

（2）小细胞肺癌H446细胞：购自ATCC细胞库。

（3）实验试剂与仪器：包括细胞培养基、血清、胰酶、MTT试剂、流式细胞仪、荧光显微镜等。

2. 方法（1）细胞培养：将H446细胞培养于含有10%胎牛血清的DMEM培养基中，置于37℃、5%CO2的培养箱中培养。

（2）旋毛虫虫体蛋白处理：将纯化的旋毛虫虫体蛋白以不同浓度处理H446细胞。

（3）细胞活性检测：采用MTT法检测细胞活性。

（4）凋亡检测：利用流式细胞仪和荧光显微镜检测细胞凋亡情况。

（5）分子生物学实验：通过PCR、Western Blot等技术检测相关基因和蛋白的表达情况。

三、结果1. 旋毛虫虫体蛋白对H446细胞活性的影响实验结果显示，不同浓度的旋毛虫虫体蛋白处理H446细胞后，细胞活性呈现浓度依赖性降低。

说明旋毛虫虫体蛋白对H446细胞具有抑制作用。

2. 旋毛虫虫体蛋白诱导H446细胞凋亡流式细胞仪和荧光显微镜检测结果显示，旋毛虫虫体蛋白处理H446细胞后，细胞凋亡率显著增加。

同时，凋亡相关蛋白如Caspase-3、Bax等表达上调，而抗凋亡蛋白Bcl-2表达下调。

3. 旋毛虫虫体蛋白诱导H446细胞凋亡的机制通过PCR和Western Blot等技术检测发现，旋毛虫虫体蛋白处理H446细胞后，相关凋亡通路如线粒体通路、死亡受体通路等被激活。

细胞周期相关基因在人类卵巢癌中过表达的机制研究

细胞周期相关基因在人类卵巢癌中过表达的机制研究细胞周期是细胞生长和分裂所必需的一系列过程，包括G1期、S期、G2期和M期。

这些过程涉及到一系列调节因子，如细胞周期蛋白激酶（CDK）、细胞周期抑制剂（CDI）和APC/C（巨大复合物）。

这些基因在维持正常细胞周期节律方面起着关键作用，而它们在许多肿瘤中常常过度表达。

卵巢癌是女性最常见的一种恶性肿瘤之一，发病率仍在不断上升。

研究显示，与正常卵巢组织相比，卵巢癌组织中细胞周期相关基因的表达水平显著变化。

其中一些基因如CDK2、CDK6、CDK4和p21等常常被认为是癌细胞增殖及转移的重要靶点。

此外，已有研究发现，许多细胞周期相关基因的调节与患上卵巢癌的风险密切相关。

例如，研究表明CDKN2A（p16）突变与卵巢癌的发生率增加有关，而MDM2、TP53和ATM等基因的突变与卵巢癌的患病率降低有关。

虽然许多基因的表达与卵巢癌的发生和发展相关，但关于其过度表达的机制仍不十分明确。

目前很多极具前瞻性的研究正在进行，以探究细胞周期基因过度表达的机制及其对肿瘤细胞转化的影响。

一些研究发现，在卵巢癌的发生和发展中，某些细胞周期调节蛋白激酶的活性增强是导致癌细胞增殖的主要机制。

例如，CDK2和CDK9通过过度的磷酸化，可以激活细胞周期的进行，同时影响蛋白质合成和DNA修复等过程。

在卵巢癌细胞中，这些过程过于活跃，从而导致细胞生长和增殖的加速。

此外，一些后续研究还发现，细胞周期调节蛋白激酶过度活跃的背后可能与某些非编码RNA（如长链非编码RNA）的不正常表达和miRNA（小干扰RNA）的削弱有关。

这些RNA可以直接或间接地调控细胞周期相关基因的表达水平，最终导致肿瘤的发生和发展。

因此，这些机制的研究可以为开发更有效的治疗方法提供新的思路。

例如，一些研究正在探究利用CDK抑制剂和miRNA的治疗潜力，以及通过干扰调节每个基因表达的RNA信号通路，来防止或减缓肿瘤细胞的生长和扩散。

西湖区高三生物第一学期期末试卷18

西湖区高三生物第一学期期末试卷一、选择题（每小题3分，共45分）1. 诺贝尔化学奖曾经授予研究细胞膜通道蛋白的科学家。

通道蛋白是一类跨越细胞膜磷脂双分子层的蛋白质,它包含离子通道蛋白和水通道蛋白。

下列相关叙述正确的是()A.通道蛋白在发挥作用时,其形状不会发生改变B.肾小管细胞通过水通道蛋白以自由扩散方式促进水的重吸收C.通道蛋白运输时没有选择性,比通道直径小的物质可自由通过D.机体可通过调节细胞膜上通道蛋白的数量或开关来调节物质的运输2. 一位鸟类学家正在研究某一种鸟的季节性迁徙行为；一位果树专家正在研究某种果树的丰产措施。

他们研究的对象分别属于（）A．种群、群落B．群落、生态系统C．物种、种群D．种群、生态系统3.4. 研究发现，将胃泌素释放肽注射到小鼠脊髓后，小鼠立刻会有抓痒行为；若在小鼠的脊髓里杀死表达胃泌素释放肽受体的神经元，不论向这些小鼠身上注射何种致痒物，小鼠都不抓痒。

下列叙述正确的是（）A. 胃泌素释放肽在突触间隙中通过自由扩散到达后膜B. 胃泌素释放肽与受体结合后，突触后膜上的Na+通道打开，Na+内流C. 将胃泌素释放肽注射到脊髓后，小鼠出现抓痒行为，属于非条件反射D. 促进胃泌素释放肽受体基因的表达，可缓解或治疗瘙痒5. 关于ATP分子的说法正确的是（）A.细胞内的吸能反应一般与ATP合成的反应相联系B.ATP与ADP相互转化速率会随细胞代谢强度变化而改变C.ATP分子是由一分子腺苷一分子核糖和三分子磷酸连接而成D.若某物质进出细胞时消耗ATP ，则该物质进出细胞的方式一定是主动运输6. 某DNA分子中，G和C之和占全部碱基总数的35.8%，其中一条链的T与C分别占该链碱基总数的32.9%和17.1%，则在它的互补链中，T与C分别占该链碱基总数的（）A．32.9%和17.1%B．31.3%和18.7% C．18.7%和31.3%D．17.1%和32.9% 7. 无尾猫是一种观赏猫。

卵巢移行细胞癌的研究进展

卵巢移行细胞癌的研究进展王琳琳(综述);李新功(审校)【摘要】卵巢移行细胞癌少见，组织形态类似于泌尿系统的尿路上皮癌，曾被认为与卵巢Brenner肿瘤同属于卵巢移行细胞肿瘤。

卵巢移行细胞癌从概念的提出就存在争议，其地位在WHO分类中也不断有变化，是一种值得关注的肿瘤。

卵巢移行细胞癌没有Brenner肿瘤成分，可伴高级别浆液性癌，具有WT-1和p53异常表达，尿路上皮标志物阴性，组织形态和免疫组织化学与高级别浆液性癌更接近，不应与Brenner肿瘤归为同一类别，而应看作是高级别浆液性癌的形态变异。

%Ovarian transitional cell carcinoma ( TCC ) is rare, which is similar to the urinary system cancer in histomorphology and has been regarded with ovarian Brenner tumor as ovarian transitional cell tumors. The concept of TCC is controversial,and its status has been changing in the WHO category,which calls for due attention. Recent studies have shown that TCCs doesn′t have Brenner tumor content,but may contain high-grade serous carcinoma without,and has abnormal expression of WT-1 and p53 with negative of urinary tract epithelial markers,the histology and immunohistochemistry are closer to the high grade serous carcinoma. Therefore,TCC should not be categorized into the same category with Brenner tumor,but should be regarded as the morphological variation of high grade serous carcinomas.【期刊名称】《医学综述》【年(卷),期】2015(000)019【总页数】3页(P3511-3513)【关键词】卵巢肿瘤;移行细胞癌;病理学【作者】王琳琳(综述);李新功(审校)【作者单位】山东省东营市人民医院病理科，山东东营257091;山东省东营市人民医院病理科，山东东营257091【正文语种】中文【中图分类】R737.31卵巢移行细胞癌少见，2003年WHO卵巢肿瘤分类中归属于来源于卵巢表面上皮的恶性肿瘤［1］，其组织形态类似于尿路发生的移行细胞癌(尿路上皮癌)。