Library of anomalous tau-tau-gamma couplings for tau+ tau- (n gamma) Monte Carlo programs
昆虫名录——精选推荐
昆⾍名录云南师范⼤学动物学野外实习名录昆⾍纲(Insecta)名录⼀、标本昆⾍名录1、蜻蜓⽬Odonata差翅亚⽬Anisoptera蜻总科Libelluloidea蜻科Libellulidea六斑曲缘蜻Palpopleura sex-maculata Fabricius⾚蜻属Crocothemis竖眉⾚蜻Sympetrum eroticum ardens McLachlan茶⾊⾚⾸缘蜻Brown Chishouyuanqing2、鳞翅⽬ Lepidoptera蛱蝶科 Nymphalidae蓝地蛱蝶Precis Orithya苎⿇⾚蛱蝶Vanessa indica Herbst粉蝶科 Pieridae菜粉蝶Pieris rapae (Linnaeus)铁⼑⽊粉蝶Catopsilia pomona Fabricius3、螳螂⽬Mantedea螳螂科Mantidea,Manteidae枯叶螳螂Deroplatys desiccate4、蜚蠊⽬Blattaria蜚蠊科Blattidae Handlirsch凹缘⼤蠊Periplaneta fulginosa5、鞘翅⽬Coleoptera龙虱科Dytiscidae龙虱Dytiscidae瓢⾍科Coccinellidae七星瓢⾍Coccinella septempunctata Linnaeus ⽔龟⾍科(⽛甲科)Hydrophilidae ⽔龟⾍ water scavenger beetle铁甲科Hispidae⽢薯腊龟甲Laccoptera quadrimaculata6、膜翅⽬ Hymenoptera胡蜂科Vespidae胡蜂属Polistes凹纹胡蜂Vespa velutina auraria Smith 7、直翅⽬Orthoptera蝗科Acrididae棉蝗属Chondracris棉蝗Chondracris rosea rosea(De Geer) 蟋蟀科Gryllidae;cricket 姬蟀Ji ophiomachus8、双翅⽬Diptera环裂亚⽬Cyclorrhapha丽蝇科Calliphoridae绿蝇属Lucilia丝光绿蝇Lucilia sericata 9、⾰翅⽬Dermaptera蠼螋科Labiduridae钳螋Forcipula sp.10、半翅⽬Hemiptera蝽科Pentatomidae; stinkbug茶翅蝽HalyomorphaPicusFabricius⼆、观察到的其他昆⾍名录1、半翅⽬Hemiptera蝽科Pentatomidae斑须蝽Dolycoris baccarum⾚条蝽Graphosoma rubrolineata猎蝽科Reduviidae猎蝽assassin bug中华猎蝽Sycanus crocevoittatus Dohrn 蝎蝽科Nepidae红娘华(蝎蝽)Nepa chinensis Hoff盲蝽科Miridae稻绿蝽Nezara vioidulaz缘蝽科Coreidae曲胫缘蝽Mictis tenetrosa⽉肩奇缘蝽Derepferyx lunata扁蝽科Aradidae暗扁蝽Aradus lugubris Fallen锹甲科Lucanidae库光胫锹甲Odontolabis curvera雷尼锹甲Nipponodorus rubrofemorathus(Vollen)西光胫锹甲Odontolabis siva Hope库光胫锹甲Odontolabis curvera Hope2、鞘翅⽬Coleoptera瓢⾍科Coccinellidae奇变瓢⾍Aiolocaria mirabilis天⽜科Cerambycidae弧斑天⽜Erythrus fortunei White黄带楔天⽜Thermistis croceocincta (Saunders )拟星天⽜Dnoplophora imitatrix (White)黄条切缘天⽜Zegriades aurovirgatus Gressitt肖丽星天⽜Anoplophora prelegans Chiang花⾦龟亚科Cetoniidae赭翅臀花⾦龟Campsiura mirabilis暗绿星花⾦龟Protadia lugubris orientalis(Medvedev)脊瘦花⾦龟Coelodera penicillata三带丽花⾦龟Eusselates orrata(Saundera)黄粉⿅花花⾦龟Dicranocephalus wallichi bowring Pascoe 鳃⾓⾦龟亚科Melolonthinae ⼤云鳃⾦龟Polyphylla laticollis Lewis丽⾦龟亚科Rutelinae⿊绿彩丽⾦龟Mimela splendens (Gllenhal)云翅彩丽⾦龟Mimela nubeculata Lin3、鳞翅⽬ Lepidoptera凤蝶科 Papilionidae凤蝶属 Papilio Linnaeus碧凤蝶Papilio bianor cramer蛱蝶科 Nymphalidae窄斑凤尾蛱蝶Polyura athamas(Drury)⼤卫绢蛱蝶Nymphalidae Calinaga Buddha⽼豹蛱蝶Argyronome laodice⼩红蛱蝶Vanessa cardui粉蝶科 Pieridae⼤展粉蝶Pieris extensa Poujade⿊纹粉蝶Pi eris melete Ménétriès⼤蚕蛾科 Saturniidae樗蚕蛾Philosamia cynthia Walker et Felder灰蝶科 Lycaenidae华灰蝶 Wagimo sulgeri (oberthur)陕灰蝶属 Shaanxiana Koiwaya sp.斑蛾科 Zygaenidae马尾松斑蛾Campylotes desgodinsi Oberthur⾈蛾科 Notodontidae栎纷⾈蛾Fentonia ocypete(Bremer)4、同翅⽬Homoptera⼤叶蝉科 Cicadellidae绿叶蝉(Chlorita sp)5、蜻蜓⽬Odonata差翅亚⽬Anisoptera蜻总科 Libelluloidea蜻科Libellulidea红蜻属Crocothemis红蜻Crocothemis servilia Drury狭翅蜻属 Potamarcha暗⾊狭翅蜻Potamarcha Obscura Rambur灰蜻属 Orthtrum褐肩灰蜻Othetrum internum6、膜翅⽬ Hymenoptera熊蜂科 Bombidae炎熊蜂Bombus ardens蜜蜂科 Apidae7、双翅⽬Diptera虻科Tabanidae纹花虻Tabanus cerealis⽑蚊科B ibionidae⽇本⽑蚊Penthethria Japinica 8、脉翅⽬Neuroptera 蛇蛉科Raphidiidae蛇蛉Raphidia notuta Fabor 9、螳螂⽬Mantodea螳科Matidae短胸⼤⼑螳Tendera brevicollis。
红树植物桐花树内生真菌Talaromyces_amestolkiae_30的次生代谢产物
第 63 卷第 2 期2024 年 3 月Vol.63 No.2Mar.2024中山大学学报(自然科学版)(中英文)ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS SUNYATSENI红树植物桐花树内生真菌Talaromyces amestolkiae 30的次生代谢产物*刘洪亮1,赵飞1,唐凤婷1,李锦俊1,张轩1,杜志云1,黄华容1,佘志刚21. 广东工业大学生物医药学院,广东广州 5100062. 中山大学化学学院,广东广州 510006摘要:对红树植物桐花树内生真菌Talaromyces amestolkiae 30的次级代谢产物进行了研究。
采用大米固体发酵培养,色谱分离技术纯化单体,ESIMS和NMR等波谱数据分析,鉴定了12个异香豆素单体化合物:aspergillumarin A(1)、aspergillumarin B(2)、 5,6-dihydroxy-3-(4-hydroxypentyl)-isochroman-1-one(3)、 mucoriso‐coumarin A(4)、 peniisocoumarin H(5)、 peniisocoumarin E(6)、 dichlorodiaportin(7)、 mucorisocoumarin C(8)、 peniiso‐coumarin G(9)、 talumarin A(10)、 5,6,8-trihydroxy-4-(1'-hydroxyethyl)-isocoumarin(11)和sescandelin(12),其中化合物4、6、7首次从篮状属真菌中分离得到。
二倍稀释法抑菌活性测试显示,化合物4、6、7有抑制金黄色葡萄球菌作用;MTT法测试细胞毒活性,表明化合物7对前列腺癌PC-3细胞和VCaP细胞有细胞毒活性。
关键词:红树林真菌;篮状菌;次级代谢产物;异香豆素类中图分类号:O629.9 文献标志码:A 文章编号:2097 - 0137(2024)02 - 0160 - 08The metabolites from mangrove endophytic fungus Talaromyces amestolkiae30LIU Hongliang1, ZHAO Fei1, TANG Fengting1, LI Jinjun1,ZHANG Xuan1, DU Zhiyun1, HUANG Huarong1, SHE Zhigang21. School of Biomedicine, Guangdong University of Technology, Guangzhou 510006, China2. School of Chemistry, Sun Yat-sen University, Guangzhou 510006, ChinaAbstract:The metabolites of the endophytic fungus Talaromyces amestolkiae30 from the mangrove plant Aegiceras corniculatum (L.) Blanco were investigated. The fungus was cultured in rice medium, the monomeric compounds were isolated and purified by the chromatographic technique, and the structures of the compounds were identified by analysis of spectroscopy such as ESIMS and NMR.Twelve known analogues of isocoumarins (1-12) were isolated and identified as aspergillumarin A (1), aspergillumarin B (2), 5,6-dihydroxy-3-(4-hydroxypentyl)-isochroman-1-one (3), mucorisocoumarin A(4), peniisocoumarin H (5), peniisocoumarin E (6), dichlorodiaportin (7), mucorisocoumarin C (8), peni‐isocoumarin G (9), talumarin A (10), 5,6,8-trihydroxy-4-(1'-hydroxyethyl)-isocoumarin (11) and sescan‐delin (12). Among them, compounds 4, 6 and 7 were obtained from the genus Talaromyces for the first time. The antibacterial activities of these compounds were tested in vitro using the twofold dilution method. Compounds 4, 6, and 7 showed inhibitory activity against Staphylococcus aureus. The cytotoxic activity was tested by the MTT assay. Compound 7 showed cytotoxicity against prostate cancer PC-3DOI:10.13471/ki.acta.snus.2023E048*收稿日期:2023 − 10 − 14 录用日期:2023 − 11 − 30 网络首发日期:2024 − 01 − 08基金项目:广东省海洋经济发展(海洋六大产业)专项资金(粤自然资合[2021]48号)作者简介:刘洪亮(1996年生),男;研究方向:制药工程;E-mail:*********************通信作者:黄华容(1978年生),女;研究方向:天然药物化学;E-mail:****************.cn第 2 期刘洪亮,等:红树植物桐花树内生真菌Talaromyces amestolkiae 30的次生代谢产物cells and VCaP cells.Key words : mangrove fungus ; Talaromyces amestolkiae ; secondary metabolites ; isocoumarin 异香豆素是一类重要的天然产物,广泛存在于自然界中,其种类繁多(Saddiqa et al.,2017; Reveglia et al.,2020;Shabir et al.,2021;Aierken et al.,2023)。
1981年克朗奎斯特被子植物分类系统
/1981年克朗奎斯特被子植物分类系统诸葛按:本分类系统大纲载于An Integrated System of Classification of Flowering Plants, Columbia University Press, 1981一书中。
各分类单位的中文译名中,除了约定俗成的科名和由科名类推得到的高级分类单位名称外,非国产科的中文译名主要来自汤彦承、路安民《被子植物非国产科汉名的初步拟订》,植物分类学报,41(3):285-304(2003)一文。
点击链接可以查看同书中对该类群的介绍。
OUTLINE OF CLASSIFICATION OF MAGNOLIOPHYTA木兰植物门分类大纲Class MAGNOLIOPSIDA木兰纲Subclass I. Magnoliidae木兰亚纲Order 1. Magnoliales木兰目Family 1. Winteraceae林仙科2. Degeneriaceae单心木兰科3. Himantandraceae瓣蕊花科4. Eupomatiaceae帽花木科5. Austrobaileyaceae木兰藤科6. Magnoliaceae木兰科7. Lactoridaceae囊粉花科8. Annonaceae番荔枝科9. Myristacaceae肉豆蔻科10. Canellaceae白樟科Order 2. Laurales樟目Family 1. Amborellaceae无油樟科2. Trimeniaceae早落瓣科3. Monimiaceae杯轴花科4. Gomortegaceae葵乐果科5. Calycanthaceae蜡梅科6. Idiospermaceae奇子树科7. Lauraceae樟科8. Hernandiaceae莲叶桐科Order 3. Piperales胡椒目Family 1. Cloranthaceae金粟兰科2. Saururaceae三白草科3. Piperaceae胡椒科Order 4. Aristolochiales马兜铃目Family 1. Aristolochiaceae马兜铃科Order 5. Illiciales八角目Family 1. Illiciaceae八角科2. Schisandraceae五味子科Order 6. Nymphaeales睡莲目Family 1. Nelumbonaceae莲科2. Nymphaeaceae睡莲科3. Barclayaceae合瓣莲科4. Cabombaceae莼菜科5. Ceratophyllaceae金鱼藻科Order 7. Ranunculales毛茛目Family 1. Ranunculaceae毛茛科2. Circaeasteraceae星叶草科3. Berberidaceae小檗科4. Sargentodoxaceae大血藤科5. Lardizabalaceae木通科6. Menispermaceae防己科7. Coriariaceae马桑科8. Sabiaceae清风藤科Order 8. Papaverales罂粟目Family 1. Papaveraceae罂粟科2. Fumariaceae紫堇科Subclass II. Hamamelidae金缕梅亚纲Order 1. Trochodendrales昆栏树目Family 1. Tetracentraceae水青树科2. Trochodendraceae昆栏树科Order 2. Hamamelidales金缕梅亚纲Family 1. Cercidiphyllaceae连香树科2. Eupteliaceae领春木科3. Platanaceae悬铃木科4. Hamamelidaceae金缕梅科5. Myrothamnaceae折扇叶科Order 3. Daphniphyllales交让木目Family 1. Daphniphyllaceae交让木科Order 4. Didymelales双蕊花目Family 1. Didymelaceae双蕊花科Order 5. Eucommiales杜仲目Family 1. Eucommiaceae杜仲科Order 6. Urticales荨麻目Family 1. Barbeyaceae钩毛叶科2. Ulmaceae榆科3. Cannabaceae大麻科4. Moraceae桑科5. Cecropiaceae伞树科6. Urticaceae荨麻科Order 7. Leitneriales塞子木目Family 1. Leitneriaceae塞子木科Order 8. Juglandales胡桃目Family 1. Rhoipteleaceae马尾树科2. Juglandaceae胡桃科Order 9. Myricales杨梅目Family 1. Myricaceae杨梅科Order 10. Fagales壳斗目Family 1. Balanopaceae橡子木科2. Fagaceae壳斗科3. Betulaceae桦木科Order 11. Casuarinales木麻黄目Family 1. Casuarinaceae木麻黄科Subclass III. Caryophyllidae石竹亚纲Order 1. Caryophyllales石竹目Family 1. Phytolaccaceae商陆科2. Achatocarpaceae玛瑙果科3. Nyctaginaceae紫茉莉科4. Aizoaceae番杏科5. Didiereaceae刺戟草科6. Cactaceae仙人掌科7. Chenopodiaceae藜科8. Amaranthaceae苋科9. Portulacaceae马齿苋科10. Basellaceae落葵科11. Molluginaceae粟米草科12. Caryophyllaceae石竹科Order 2. Polygonales蓼目Family 1. Polygonaceae蓼科Order 3. Plumbaginales白花丹目Family 1. Plumbaginaceae白花丹科Subclass IV. Dilleniidae五桠果亚纲Order 1. Dilleniales五桠果目Family 1. Dilleniaceae五桠果科2. Paeoniaceae芍药科Order 2. Theales山茶目Family 1. Ochnaceae金莲木科2. Sphaerosepalaceae球萼树科3. Sarcolaenaceae苞杯花科4. Dipterocarpaceae龙脑香科5. Caryocaraceae油桃木科6. Theaceae山茶科7. Actinidiaceae猕猴桃科8. Scytopetalaceae革瓣花科9. Pentaphylacaceae五列木科10. Tetrameristaceae四出花科11. Pellicieraceae假红树科12. Oncothecaceae钩药茶科13. Marcgraviaceae蜜囊花科14. Quiinaceae绒子树科15. Elatinaceae沟繁缕科16. Paracryphiaceae盔瓣花科17. Medusagynaceae伞果树科18. Clusiaceae藤黄科Order 3. Malvales锦葵目Family 1. Elaeocarpaceae杜英科2. Tiliaceae椴树科3. Sterculiaceae梧桐科4. Bombacaceae木棉科5. Malvaceae锦葵科Order 4. Lecythidales玉蕊目Family 1. Lecythidaceae玉蕊科Order 5. Nepenthales猪笼草目Family 1. Sarraceniaceae瓶子草科2. Nepenthaceae猪笼草科3. Droseraceae茅膏菜科Order 6. Violales堇菜目Family 1. Flacourtiaceae刺篱木科2. Peridiscaceae围盘树科3. Bixaceae红木科4. Cistaceae半日花科5. Huaceae蒜树科6. Lacistemataceae裂药花科7. Scyphostegiaceae杯盖花科8. Stachyuraceae旌节花科9. Violaceae堇菜科10. Tamaricaceae柽柳科11. Frankeniaceae瓣鳞花科12. Dioncophyllaceae双钩叶科13. Ancistrocladaceae钩枝藤科14. Turneraceae时钟花科15. Malesherbiaceae王冠草科16. Passifloraceae西番莲科17. Achariaceae脊脐子科18. Caricaceae番木瓜科19. Fouquieriaceae澳可第罗科20. Hoplestigmataceae干戈柱科21. Cucurbitaceae葫芦科22. Datiscaceae四数木科23. Begoniaceae秋海棠科24. Loasaceae刺莲花科Order 7. Salicales杨柳目Family 1. Salicaceae杨柳科Order 8. Capparales白花菜目Family 1. Tovariaceae鲜芹味科2. Capparaceae白花菜科3. Brassicaceae十字花科4. Moringaceae辣木科5. Resedaceae木犀草科Order 9. Batales肉穗果目Family 1. Gyrostemonaceae环蕊木科2. Bataceae肉穗果科Order 10. Ericales杜鹃花目Family 1. Cyrillaceae翅萼树科2. Clethraceae桤叶树科3. Grubbiaceae毛盘花科4. Empetraceae岩高兰科5. Epacridaceae澳石南科6. Ericaceae杜鹃花科7. Pyrolaceae鹿蹄草科8. Monotropaceae水晶兰科Order 11. Diapensiales岩梅目Family 1. Diapensiaceae岩梅科Order 12. Ebenales柿树目Family 1. Sapotaceae山榄科2. Ebenaceae柿树科3. Styracaceae安息香科4. Lissocarpaceae光果科5. Symplocaceae山矾科Order 13. Primulales报春花目Family 1. Theophrastaceae拟棕科2. Myrsinaceae紫金牛科3. Primulaceae报春花科Subclass V. Rosidae蔷薇亚纲Order 1. Rosales蔷薇目Family 1. Brunelliaceae槽柱花科2. Connaraceae牛栓藤科3. Eucryphiaceae落帽花科4. Cunoniaceae南蔷薇科5. Davidsoniaceae大维逊李科6. Dialypetalanthaceae拟素馨科7. Pittosporaceae海桐花科8. Byblidaceae二型腺毛科9. Hydrangeaceae八仙花科10. Columelliaceae弯药树科11. Grossulariaceae茶藨子科12. Greyiaceae鞘叶树科13. Bruniaceae鳞叶树科14. Anisophylleaceae异叶木科15. Alseuosmiaceae假海桐科16. Crassulaceae景天科17. Cephalotaceae囊叶草科18. Saxifragaceae虎耳草科19. Rosaceae蔷薇科20. Neuradaceae两极孔草科21. Crossosomataceae流苏子科22. Chrysobalanaceae可可李科23. Surianaceae海人树科24. Rhabdodendraceae棒状木科Order 2. Fabales豆目Family 1. Mimosaceae含羞草科2. Caesalpiniaceae云实科3. Fabaceae豆科Order 3. Proteales山龙眼目Family 1. Elaeagnaceae胡颓子科2. Proteaceae山龙眼科Order 4. Podostemales川薹草目Family 1. Podostemaceae川薹草科Order 5. Haloragales小二仙草目Family 1. Haloragaceae小二仙草科2. Gunneraceae大叶草科Order 6. Myrtales桃金娘目Family 1. Sonneratiaceae海桑科2. Lythraceae千屈菜科3. Penaeaceae管萼木科4. Crypteroniaceae隐翼科5. Thymelaeaceae瑞香科6. Trapaceae菱科7. Myrtaceae桃金娘科8. Punicaceae石榴科9. Onagraceae柳叶菜科10. Oliniaceae方枝树科11. Melastomataceae野牡丹科12. Combretaceae使君子科Order 7. Rhizophorales红树目Family 1. Rhizophoraceae红树科Order 8. Cornales山茱萸目Family 1. Alangiaceae八角枫科2. Nyssaceae蓝果树科3. Cornaceae山茱萸科4. Garryaceae丝缨花科Order 9. Santalales檀香目Family 1. Meduandraceae毛丝花科2. Dipentodontaceae十齿花科3. Olacaceae铁青树科4. Opiliaceae山柚子科5. Santalaceae檀香科6. Misodendraceae羽果科7. Loranthaceae桑寄生科8. Viscaceae槲寄生科9. Eremolepidaceae绿乳科10. Balanophoraceae蛇菰科Order 10. Rafflesiales大花草目Family 1. Hydnoraceae腐臭草科2. Mitrastemonaceae帽蕊草科3. Rafflesiaceae大花草科Order 11. Celastrales卫矛目Family 1. Geissolomataceae四棱果科2. Celastraceae卫矛科3. Hippocrateaceae翅子藤科4. Stackhousiaceae异雄蕊科5. Salvadoraceae刺茉莉科6. Aquifoliaceae冬青科7. Icacinaceae茶茱萸科8. Aextoxicaceae毒羊树科9. Cardiopteridaceae心翼果科10. Corynocarpaceae棒果木科11. Dichapetalaceae毒鼠子科Order 12. Euphorbiales大戟目Family 1. Buxaceae黄杨科2. Simmondsiaceae油蜡树科3. Pandaceae攀打科4. Euphorbiaceae大戟科Order 13. Rhamnales鼠李目Family 1. Rhamnaceae鼠李科2. Leeaceae火筒树科3. Vitaceae葡萄科Order 14. Linales亚麻目Family 1. Erythroxylaceae古柯科2. Humiriaceae树脂核科3. Ixonanthaceae粘木科4. Hugoniaceae亚麻藤科5. Linaceae亚麻科Order 15. Polygalales远志目Family 1. Malpighiaceae金虎尾科3. Trigoniaceae三角果科4. Tremandraceae孔药木科5. Polygalaceae远志科6. Xanthophyllaceae黄叶树科7. Krameriaceae刺球果科Order 16. Sapindales无患子目Family 1. Stapyleaceae省沽油科2. Melianthaceae蜜花科3. Bretschneideraceae钟萼木科4. Akaniaceae叠珠树科5. Sapindaceae无患子科6. Hippocastanaceae七叶树科7. Aceraceae槭树科8. Burseraceae橄榄科9. Anacardiaceae漆树科10. Julianiaceae三柱草科11. Simaroubaceae苦木科12. Cneoraceae叶柄花科13. Meliaceae楝科14. Rutaceae芸香科15. Zygophyllaceae蒺藜科Order 17. Geraniales牻牛儿苗目Family 1. Oxalidaceae酢浆草科2. Geraniaceae牻牛儿苗科3. Limnanthaceae沼花科4. Tropaeolaceae旱金莲科5. Balsaminaceae凤仙花科Order 18. Apiales伞形目Family 1. Araliaceae五加科2. Apiaceae伞形科Subclass VI. Asteridae菊亚纲Order 1. Gentianales龙胆目Family 1. Loganiaceae马钱科2. Retziaceae异轮叶科3. Gentianaceae龙胆科4. Saccifoliaceae勺叶木科5. Apocynaceae夹竹桃科6. Asclepiadaceae萝藦科Order 2. Solanales茄目Family 1. Duckeodendraceae核果茄科2. Nolanaceae假茄科3. Solanaceae茄科5. Cuscutaceae菟丝子科6. Menyanthaceae睡菜科7. Polemoniaceae花荵科8. Hydrophyllaceae田基麻科Order 3. Lamiales唇形目Family 1. Lennoaceae多室花科2. Boraginaceae紫草科3. Verbenaceae马鞭草科4. Lamiaceae唇形科Order 4. Callitrichales水马齿目Family 1. Hippuridaceae杉叶藻科2. Callitrichaceae水马齿科3. Hydrostachyaceae水穗草科Order 5. Plantaginales车前目Family 1. Plantaginaceae车前科Order 6. Scrophulariales玄参目Family 1. Buddlejaceae醉鱼草科2. Oleaceae木犀科3. Scrophulariaceae玄参科4. Globulariaceae肾药花科5. Myoporaceae苦槛蓝科6. Orobanchaceae列当科7. Gesneriaceae苦苣苔科8. Acanthaceae爵床科9. Pedaliaceae胡麻科10. Bignoniaceae紫葳科11. Mendonciaceae对叶藤科12. Lentibulariaceae狸藻科Order 7. Campanulales桔梗目Family 1. Pentaphragmataceae五膜草科2. Sphenocleaceae楔瓣花科3. Campanulaceae桔梗科4. Stylidiaceae花柱草科5. Donatiaceae陀螺果科6. Brunoniaceae蓝针花科7. Goodeniaceae草海桐科Order 8. Rubiales茜草目Family 1. Rubiaceae茜草科2. Theligonaceae假牛繁缕科Order 9. Dipsacales川续断目Family 1. Caprifoliaceae忍冬科2. Adoxaceae五福花科3. Valerianaceae败酱科Order 10. Calycerales萼角花目Family 1. Calyceraceae萼角花科Order 11. Asterales菊目Family 1. Asteraceae菊科Class LILIOPSIDA百合纲Subclass I. Alismatidae泽泻亚纲Order 1. Alismatales泽泻目Family 1. Butomaceae花蔺科2. Limnocharitaceae黄花蔺科3. Alismataceae泽泻科Order 2. Hydrocharitales水鳖目Family 1. Hydrocharitaceae水鳖科Order 3. Najadales茨藻目Family 1. Aponogetonaceae水蕹科2. Scheuchzeriaceae冰沼草科3. Juncaginaceae水麦冬科4. Potamogetonaceae眼子菜科5. Ruppiaceae川蔓藻科6. Najadaceae茨藻科7. Zannichelliaceae角果藻科8. Posidoniaceae波喜荡科9. Cymodoceaceae丝粉藻科10. Zosteraceae大叶藻科Order 4. Triuridales霉草目Family 1. Petrosaviaceae无叶莲科2. Triuridaceae霉草科Subclass II. Arecidae槟榔亚纲Order 1. Arecales槟榔目Family 1. Arecaceae槟榔科Order 2. Cyclanthales巴拿马草目Family 1. Cyclanthaceae巴拿马草科Order 3. Pandanales露兜树目Family 1. Pandanaceae露兜树科Order 4. Arales天南星目Family 1. Araceae天南星科2. Lemnaceae浮萍科Subclass III. Commelinidae鸭跖草亚纲Order 1. Commelinales鸭跖草目Family 1. Rapateaceae瑞碑题雅科2. Xyridaceae黄眼草科3. Mayacaceae花水藓科4. Commelinaceae鸭跖草科Order 2. Eriocaulales谷精草目Family 1. Eriocaulaceae谷精草科Order 3. Restionales帚灯草目Family 1. Flagellariaceae须叶藤科2. Joinvilleaceae拟苇科3. Restionaceae帚灯草科4. Centrolepidaceae刺鳞草科Order 4. Juncales灯心草目Family 1. Juncaceae灯心草科2. Thurniaceae梭子草科Order 5. Cyperales莎草目Family 1. Cyperaceae莎草科2. Poaceae禾本科Order 6. Hydatellales独蕊草目Family 1. Hydatellaceae独蕊草科Order 7. Typhales香蒲目Family 1. Sparganiaceae黑三棱科2. Typhaceae香蒲科Subclass IV. Zingiberidae姜亚纲Order 1. Bromeliales凤梨目Family 1. Bromeliaceae凤梨科Order 2. Zingiberales姜目Family 1. Streliziaceae鹤望兰科2. Heliconiaceae蝎尾蕉科3. Musaceae芭蕉科4. Lowiaceae兰花蕉科5. Zingiberaceae姜科6. Costaceae闭鞘姜科7. Cannaceae美人蕉科8. Marantaceae竹芋科Subclass V. Liliidae百合亚纲Order 1. Liliales百合目Family 1. Philydraceae田葱科2. Pontederiaceae雨久花科3. Haemodoraceae血草科4. Cyanastraceae蓝星科5. Liliaceae百合科6. Iridaceae鸢尾科7. Velloziaceae翡若翠科8. Aloeaceae芦荟科9. Agavaceae龙舌兰科10. Xanthorrhoeaceae黄脂木科11. Hanguanaceae钵子草科12. Taccaceae蒟蒻薯科13. Stemonaceae百部科14. Smilacaceae菝葜科15. Dioscoreaceae薯蓣科Order 2. Orchidales兰目Family 1. Geosiridaceae地鸢尾科2. Burmanniaceae水玉簪科3. Corsiaceae腐蛛草科4. Orchidaceae兰科木兰植物门(MAGNOLIOPHYTA)Cronquist, Takhtajan & Zimmermann 1966又名被子植物或有花植物维管束植物,一般具有根、茎和叶,茎的中央柱(central cylinder)有叶隙或分散的维管束;木质部通常(但并非总是)含有导管的一部分,至少在根部如此;韧皮部有定数的筛管和伴胞。
跨物种扩增筛选获得蓝马鸡Crossoptilon auritum微卫星多态分子标记英文
Chinese Birds 2012,3(2):103–107SHORT COMMUNICA TIONReceived 04March 2012;accepted 10May 2012Author for correspondence (Zhengwang Zhang)E-mail:zzw@*Y ang L iuPresent address:State Key L aboratory of Biocontrol and School of LifeSciences,Sun Y at-Sen University,Guangzhou 510275,ChinaA panel of polymorphic microsatellites in the Blue Eared P heasant (Crossoptilon auritum)developed by cross-species amplificationLangyu GU 1,Y ang LIU 2,*,N ngA NG 1,Zhengwang ZHA NG 1,1MOE K ey Laboratory for Biodiv ersity Sciences and Ecological Engineering,College of L ife Sciences,Beijing Normal University ,Beijing 100875,China2Computational and Molecular Population Genetics,Institute of Ecology and Ev olution,Univ ersity of Bern,Baltzerstrasse 6,3012,Bern,SwitzerlandAbstr act Polymorphic microsatellites are among the versatile genetic markers in molecular ecology studies.In contrast to de no vo isolation of microsatellites from target species,cross-species ampli ca-tion is a cost-effective approach for a fast development of microsatellite markers from closely related taxa.In our study ,we cross-ampli ed a panel of poly morphic microsatellite markers for the Blue Eared Pheasant (Crosso ptilon auritum),a species endemic to China of considerable conservation con-cern.We obtained 11polymorphic microsatellite markers selected from 112candidate loci,originally isolated from other Galliforme species.This panel of makers has shown moderate to high lev els of polymorphism and include a Z-chromosomal linkage locus.We carried out preliminary analy ses of parentage among captive individuals with a known pedigree using this new panel of microsatellites.Our results suggest that the high utility of these markers may be powerful tools for studies in conser-vation genetics of eared-pheasants and other endang ered Galliforme species.Keywords Crossoptilon auritum,microsatellites,cross-species ampli cation,Z-chromosomal linkag eInt roductionEndemic species have long been a key focus in conser-vation efforts (Myers et al.,2000),given that the levelof endemics might be positiv ely correlated with species richness (Lamoreux et al.,2006).Besides,endemic spe-cies with limited dispersal capacity might be sensitive to changes in local climate,or vulnerable to invasive spe-cies (Ohlem üller et al.,2008).A good understanding of ev olutionary processes such as population subdivisions,changes of effective population size and genetic connec-tivity of endemic species would shed light on evolution-ary processes as well as on conservation manag ement.The Blue Eared Pheasant (Crossoptilon auritum),belonging to Phasianidae,Galliformes,is a rare and en-demic pheasant species in western China (L ei and L u,2006).Its wild populations are found at Helan Moun-Chinese Birds2012,3(2):103–107 104tain,as well as along the eastern edge of the Qinghai-Tibetan Plateau(QTP),cov ering Qing hai,Gansu and Sichuan provinces(L ei and L u,2006).A lthough previ-ous studies have been carried out on the biology and ecology of C.auritum(Sun et al.,2005;Li et al.,2009; Wu and L iu,2010),a thorough assessment of genetic diversity is urg ently needed to assess the population vi-ability of this species.Furthermore,apart from C.auri-tum,the genus Crosso ptilon includes three other species, i.e.,C.mantchuricum,C.harmani and C.cro ssoptilo n. These species are endemic to China(Zheng,2011)and all are listed on the IUCN Red L ist of Threatened Spe-cies(IUCN2011)because of a rapid decline in the size of their population,caused by habitat fragmentation and hunting(Lei and Lu,2006).Giv en these concerns, obtaining molecular markers is a prerequisite in un-derstanding the genetic background of C.auritum and might be useful for population genetic studies in Cros-so ptilon species.Microsatellites are powerful tools for conservation genetic studies such as population genetics,mating systems and inv estigations into kinship(Primmer et al., 2005;Karl et al.,2011).Compared with isolated novel microsatellite markers,cross-species microsatellite am-pli cation from closely related species is cost-effective (Zane et al.,2002).More importantly,it has been sug-gested that this method has successfully worked among species belonging to the same genera,different genera and even different families(Barbaráet al.,2007;Huang and L iao,2010).Given that numerous microsatellite markers have been dev eloped for various Phasianidae species(Cheng et al.,1995;Wang et al.,2009;Zhou and Zhang,2009),we attempted to establish microsatellite markers for C.auritum through cross-species ampli-cation from a large number of marker candidates.In order to test the effectiveness of these markers,we also carried out preliminar y parentage analysis among cap-tive individuals of known pedigree.Mate ria ls and methodsA total of20C.auritum blood samples from brachial veins were collected to develop microsatellite loci by cross-species ampli cation,nine of which were from the Linxia Zoo in Gansu Province and the other11 from Huzhu in Qinghai Province,China.Additionally, nine individual birds from two families of the Beijing Wildlife Park in Daxing and Beijing Zoo were used to conduct parentage analy sis.We have detailed the known pedig ree of these two families of birds.Genomic DNA was extracted using DNA extraction kits(Tian Gen Biotech,Beijing,China).The cross-spe-cies microsatellite markers(Table1)came from various Galliforme species,including16loci from Meleag risgal-lopavo(Burt et al.,2003),30from T rag opan temminck ii (Zhou and Zhang,2009),20from Syrmatic us reevesii (Wang et al.,2009),41from Gallus gallus(Cheng et al., 1995;Dawson et al.,2010),four from C.mantchuric um (Zhao et al.,Beijing Normal Univ ersity,unpublished results)and one from Syrmaticus mikado(S.H.L i,T ai-wan Normal Univ ersity,unpublished results).After ltering out those loci with poor cross-ampli cation, the polymorphism of the remaining pairs were tested with either6-FA M or HEX uorescent dyelabeled on 5′of a single forward primer.Polymerase chain reac-tion(PCR)was carried out in a10L reaction system containing100ng DNA,0.25L of each primer,1L of a10×PCR buffer,1.5mM Mg Cl2,0.2mM dNTP mix and0.75U T aq polymerase(Takara,Japan).The reac-tion was denatured at94°C for5min,followed by40 cy cles at94°C for30s,a touch-down annealing process from58–47°C,reducing in steps of0.5°C per cycle and another20cycles annealing at47°C and then72°C for 50s,with a nal extension at72°C for5min.Fragment analy sis was conducted on an ABI PRISM3100Genetic Analyzer using the GeneMapper software(Applied Biosystems)with ROX-500as the standard for size.We conducted PCR ampli cation and fragment analysis at least twice to ensure the accuracy of individual geno-types.Sequencing of selected homozygotes was also conducted in both directions with ampli cation prim-ers(BGI Bio T ech,Beijing,China)to ensure that the products were genuine microsatellites.We blast the acquired microsatellite sequences from C.auritum on the chicken genome in GenBank to nd their locations and to inv estigate the potential linkages among the loci.If some loci were mapped in sex chro-mosome,we carried out sex ing identi cation with ourLangyu Gu et al.Microsatellite DNA loci in Blue Eared Pheasant105sample set by using primer sex1/sex2(Wang and Zhang,2009)to cross-validate the sex-linkage loci.We tested observed heterozygosity(HO),expectedheterozygosity(HE),the number of allele per locus, the Hardy-Weinberg equilibrium(HWE)and linkage-disequilibrium in each population using A rlequinv.3.11 (Excof er et al.,2005).W e calculated the frequencies of null alleles by using FreeNA(Chapuis and Estoup, 2007).High polymorphic markers with HWE were used for paternity tests in CERVUS3.0with100000 times simulations to estimate their resolving power. Signi cant levels were recorded after application of the sequential Bonferroni correction(Rice,1989;Ex cof er et al.,2005).ResultsSixty-two(55.4%)of the112cross-species markers could be ef ciently ampli ed in C.auritum,while11 (17.7%)of the62markers had a moderate to high level of polymorphism(3–11loci),with the expected het-erozyg osities ranging from0.42to0.89(T able1).Locus 4C12was found to be homozyg ous in heterogametic fe-males and heterozy gous in56.2%of the males(n=28) and thus most likely to link with the Z chromosome. Three loci(1H4,2420and4F8)were not targ eted on the chicken genome.A ll loci were at the HWE;neither linkage disequilibrium among pairs of loci nor null al-leles was found.The parentage analysis results showedTable1Characterization of11microsatellite lociL ocus Primer sequences(5′–3′)A ccessionNo.Repeatmotifsn NASize range(bp)HOHESpecies origin Chromosome2580F:TTAACCTA TCAGGTCGTTGCG AL592580(CA)n208191–213 1.000.80M.gallopavo21 R:CA GTGCACA TGCA GGCA G3D2F:TCTCTGA CGTA TCGCA TCT FJ221373(GT)n196286–3040.470.58S.reevesii4 R:A CTTCCCCTGGTAAA CT1H4F:TGAACA AGTGA GGCGGAGC/(TG)n2010127–1610.650.81S.reevesii/ R:CTGCACA CAGCCCGAA GC2420F:CA TCA TCTGCCAA TGCA GAGG/(TTTA)n204118–1420.550.54S.mikado/ R:A AGCCCA TA TA TGCTTCCTGG4H1F:TA TGAAA CAGA CTTAA TCC FJ221388(GTTT)n204203–2110.850.67S.reevesii1 R:TGCAGCA TTTGA GT AAC5C9F:TA TGGGAA A TGTGTACCTTT A GQ184557(CA)n2010221–2590.950.89 C.mantchuricum10 R:TCCA GGCAA CACGTAA CATT06F:TGAGAGA TTTTGACCCA GQ181183(CA)n207225–2370.850.83T.temminckii6 R:CAA GACTTCA CCCTA CAGA TA4F8F:GTGGCA TGCCTAGTA GA TGTT/(A C)n2011186–2140.750.88 C.mantchuricum/ R:CCCTGTGGTA CGAACTGTCSR11F:A TCAA T A TGGACTGCTCCGT FJ221381(TG)n205210–2480.550.58S.reevesii17 R:TCCTTCA AGGCCAAGTG5H7F:CCAA GAGGGA GGCA CACGTTC U60782(TG)n203186–1940.550.42G.gallus8 R:A GCCA TAAA T AAGCAAA CGC4C12F:A T AGGCGGACA GAGGA T AGA FJ221385(CA)n204160–1700.300.59S.reevesii Z R:CCCCGCA TCGAGGTGNotes:n is the number of successfully genotyped individuals and NA the number of alleles.HOis observed heterozygosity and HEexpected heterozygosity.Chinese Birds2012,3(2):103–107 106that if neither parent was known,the successful assig n-ment rates were98%at a95%con dence lev el and 98%at an80%con dence level.Paternity test results are consistent with known pedigrees.DiscussionThe phylogenetic relationship between the original and target species seems to affect the success of cross-species ampli cation(Primmer et al.,1996).In our analyses, cross-species ampli cation from Crossoptilon was the most effective(2/4=50%),followed by those from Syrmaticus(6/21=28.6%),Meleag ris(1/16=6.25%), Trag opan(1/30= 3.0%)and Ga llus(1/41=2.4%). Phylogenetic analysis showed a successiv e relationship between Crossoptilon and the genera mentioned earlier (Kimball et al.,2011),indicating that evolutionary re-lationships may play an important role in cross-species ampli cation of microsatellite markers within Gallifor-mes.A lthough large numbers of microsatellite markers are found in autosomes,Z-linked microsatellites markers are still rarely av ailable,ev en in the well-characterized chicken genome(Groenen et al.,2000).One Z-linked marker TUT,originally isolated from the T etrao uro gal-lus(Seg elbacher et al.,2000,Wang et al.,2011),failed to be ampli ed in chickens and mig ht be speci c for T etr-aoninae grouse.In the present study,the Z-linked poly-morphic locus4C12seems to hav e general application in different Galliforme species and might yet provide a valuable tool in kinship and demographic analyses combined with other loci.Acknowledgements The study was supported by the National Key Project of the Scienti c and Technical Supporting Programs Funded by the Ministry of Science and T echnology of China (2012BA C01B06)and the National Natural Science Founda-tion of China(No.30570234).We thank Jiliang X u,Xinli Zhao, Jiang Chang and Ying Liu for the collection of samples,as well as administrations of the Y inchuan Zoo in Ningxia A utonomous Region,the Xining Zoo in Qinghai Province,the Linxia Dongjiao Zoo in Gansu Province and the Beijing Wildlife Park and Beijing Zoo for providing samples.We thank Xinli Zhao for provid-ing unpublished markers from Crossoptilon mantchuricum and Shou-Hsien Li for providing unpublished markers from Syrmati-cus mikado.We thank Lu Dong,Xiangjiang Zhan,Ning Wang and Y ingying Liu for guidance with data analysis.ReferencesBarbaráT,Palma-Silva C,Paggi GM,Bered F,Fay MF,L exer C.2007.Cross-species transfer of nuclear microsatellite markers: potential and limitations.Mol Ecol,16:3759–3767.Burt DW,Morrice DR,Sewalem A,Smith J,Paton IR,Smith EJ, Bentley J,Hocking PM.2003.Preliminary linkage map of the T urkey(Meleagris gallopavo)based on microsatellite markers.A nim Genet,34:399–409.Chapuis MP,Estoup A.2007.Microsatellite null alleles and esti-mation of population differentiation.Mol Biol Evol,24:621–631.Cheng HH,Levin I,V allejo RL,Khatib H,Dodgson JB,Critten-den LB,Hillel J.1995.Development of a genetic map of the chicken with markers of high utility.Poult Sci,74:1855–1874. Cheng TH.1978.Fauna Sinica Series V ertebrata A ves V ol.4:Gal-liformes.Science Press,Beijing.Dawson DA,Horsburgh GJ,Küpper C,Stewart IRK,Ball AD, Durrant KL,Hansson B,Bacon I,Bird S,Klein,Krupa A P, L ee JW,Martín-Gálvez D,Simeoni M,Smith G,Spurgin LG, Burke T.2010.New methods to identify conserved microsatel-lite loci and develop primer sets of high cross-species utility–as demonstrated for birds.Mol Ecol Resour,10:475–494. Excof er L,Laval G,Schneider S.2005.A RLEQUIN(version3.01):an integrated software package for population geneticdata analysis.E vol Bioinform Online,1:47–50.Groenen MA,Cheng HH,Bumstead N,Benkel BF,Briles WE, Burke T,Burt DW,Crittenden LB,Dodgson J,Hillel J,L amont S,Leon A P,Soller M,T akahashi H,Vignal A.2000.A consensus linkagemap of the chicken genome.Genome Res,10:137–147. Huang ZH,Liao X J.2010.Cross-species ampli cation and char-acterization of microsatellite DNA loci from Gallus gallus in Bambusicola thoracica.Chinese Birds,1:74–76.IUCN.2011.IUCN Red L ist of Threaten ed Species.V ersion 2011.2...A ccessed15Ocotober2009. Karl SA,Castro A LF,L opez JA,Charvet P,Burgess GH.2011.Phylogeography and conservation of the bull shark(Carcha-rhinus leucas)inferred from mitochondrial and microsatellite DNA.Conserv Genet,12:371–382.Kimball RT,Mary CMS,Braun EL.2011.A macroevolutionaryLangyu Gu et al.Microsatellite DNA loci in Blue Eared Pheasant107perspective on multiple sexual traits in the Phasianidae(Gal-liformes).Int J Evol Biol,2011:423938.Lamoreux JF,Morrison JC,Ricketts TH,Olson DM,Dinerstein E,McK night MW,Shugart HH.2006.Global test of biodiver-sity concordance and the importance of endemism.Nature, 440:212–214.Lei FM,L u TC.2006.China Endemic Birds.Science Press,Bei-jing.Li ZG,Hu TH,Zhai H.2009.Habitat selection in spring and summer of Blue-eared Pheasant in Helan Mountain National Nature Reserve.Chinese J Wildl,30:310–313.(in Chinese) Myers N,Mittermeier RA,Mittermeier CG,Fonseca GAB,Kent J.2000.Biodiversity hotspots for conservation priorities.Nature, 403:853–858.Ohlemüller R,Anderson BJ,Araújo MB,Butchart SHM,K udrna O,Ridgely RS,Thomas CD.2008.The coincidence of climatic and species rarity:high risk to small-range species from cli-matechange.Biol Lett,4:568–572.Primmer CR,Moller A P,Ellegren H.1996.A wide-range survey of cross-species microsatellite ampli cation in birds.Mol Ecol, 5:365–378.Primmer CR,Painter JN,K oskinen MT,Palo JU,Meril J.2005.Factors affecting avian cross-species microsatellite ampli ca-tion.J A vian Biol,36:348–360.Rice WR.1989.Analyzing tables of statistical tests.E volution, 43:223–225.Segelbacher G,Paxton RJ,Steinbruck G,Trontelj P,Storch I.2000.Characterization of microsatellites in capercaillie T etrao urogallus.Mol Ecol,9:1919–1952.Sun Y H,Jia CX,Fang Y,Zheng GM.2005.Preliminary study on the incubation rhythms of the Blue-eared Pheasant at Lian-huashan,Gansu,China.Chinese J Zool,40(4):29–33.(in Chi-nese with English abstract)Wang J,Y ang C,Zhu L,Jia CX,Sun YH.2011.Sex-linkage identi-cation of microsatellite TUT1.Chinese J Appl Environ Biol, 17:404–406.(in Chinese with English abstract)Wang N,Liu Y,Zhang ZW.2009.Characterization of nine micro-satellite loci for a globally vulnerablespecies,Reeves’s Pheasant (Syrmaticus reevesii).Conserv Genet,10:1511–1514.Wang N,Zhang ZW.2009.The novel primers for sex identi ca-tion in the brown eared-pheasant and their application to other species.Mol Ecol Resour,9:186–188.Wu Y H,L iu NF.2010.The biology research of Crossoptilon auri-tum.Forest By-prod Special China,4:99–102.(in Chinese) Zane L,Bargelloni L,Patarnello T.2002.Strategies for microsat-ellite isolation:a review.Mol Ecol,11:1–16.Zheng GM.2011.A Checklist on the Classi cation and Distribu-tion of theBirds of China.2nd edition.Science Press,Beijing. Zhou ZT,Zhang Y Y.2009.Isolation and characterization of mi-crosatellite markers for T emminck’s T ragopan(Tragopan tem-minckii).Conserv Genet,10:1633–1635.跨物种扩增筛选获得蓝马鸡(Crossopt ilon auritum)微卫星多态分子标记谷浪屿1,刘阳2,3,王宁1,张正旺1(1北京师范大学生命科学学院,生物多样性与生态工程教育部重点实验室,北京,100875;2瑞士伯尔尼大学生态与进化研究所进化生态计算和分子群体遗传学组,伯尔尼,3012;3中山大学生命科学学院,有害生物控制与资源利用国家重点实验室,广州,510275)摘要:微卫星是分子生态学研究常用分子标记之一。
内蒙古的腹菌
腹菌(gasteroid fungi)是指蘑菇亚门(Agari⁃comycotina)中菌体产孢组织(子实层体)常被包裹(闭果式)、传统上被称为腹菌(Gasteromycetes)的类群[1]。
腹菌子实体大多数腐生于地面或地下,有的在地下形成,成熟时露出地面,有的则永久留在地下,也有木生或粪生的,还有的与树木形成外生菌根。
许多腹菌具有重要的经济价值,如长裙竹荪Phallus indusiatus 是著名的食药兼用菌,已广为栽培。
秃马勃属Calvatia 和马勃属Lycoperdon 一些种幼嫩的子实体可食用,成熟后药用[1,2]。
早期邓叔群[3]、戴芳澜[4]和Liu [5]系统报道了我国腹菌物种资源。
目前,《中国真菌志》腹菌类已出版四卷册[6-9],共报道我国腹菌25科66属323种(含变种和变型)。
真菌在适应特殊气候(如干旱)和传媒(如啮齿类动物)等因子的演化中,大量没有亲缘关系或亲缘关系很远的类群出现了相似的结构称为趋同演化。
担子菌的腹菌化和子囊菌的块菌化就是十分典型的例证[10]。
分子生物学研究表明,在传统的腹菌中,有些种类是担子菌腹菌化的结果,如陀螺青褶伞Chlorophyllum agaricoides [≡Endoptychum agari⁃coides ][11,12]和荒漠斑褶菇Panaeolus desertorum [≡Galeropsis desertorum ][13]。
近年来,国内Li 等[14,15]和Sang 等[16]陆续发表了多种腹菌化的红菇和乳菇新种。
许雯珺等[17]在西藏自治区林芝县发现了腹菌化的氯味红菇(氯味地红菇)Russula chlorineolens Trappe &T.F.Elliott [≡Macowanites chlorinosmus A.H.Sm.&Trappe]。
王锋尖等[18]在湖北省十堰市张湾区发现了腹菌化的棱柱孢粉褶菌Entoloma prismaticum Hir.Sasaki,A.Kinosh.&K.Nara。
海马的结构及功能
• ( 3 ) 杏 仁 被 盖 束 amygdalo-tegmental tract:终止于下丘脑外侧区,黑质、蓝斑等。
• ( 4 ) 杏 仁 皮 质 投 射 amygdalo-cortical projection:投射至眶额回、颞上、中、下回。
• 根据细胞形态,不同皮质区发育旳差别以 及 多 种 纤 维 通 路 旳 不 同 , Lorente do No{1934}把海马又提成CA1、CA2、CA3、 CA4 四 个 扇 形 区 。 CA4 位 于 齿 状 回 门 内,CA3区内有最大旳锥体细胞,CA3区由 大锥体细胞构成, CA1区是邻近下托旳部 分,由小锥体细胞构成,CA2区是移行区, 由大和小锥体细胞构成。
核等。外侧隔核接受大量从海马经穹窿来旳传
入纤维。
• 1.传入纤维
•
〈1〉发自海马旳纤维经穹隆止于外侧隔
核。
•
〈2〉发自杏仁体旳纤维经斜角带或终纹
止于隔核。
• 〈3〉发自中脑网状构造,黑质、蓝斑、中 缝核与下丘脑核旳纤维终止于内侧隔核。
• 〈4〉前穿质发纤维经内侧嗅纹至隔。
• (5)额叶新皮质和扣带回发纤维至隔。
• 3.海马构造旳纤维联络
•
〈1〉传入
•
①丰富旳传入来自内嗅区。
•
②扣带回发纤维经扣带束直接终止
于海马和或经内嗅区中继后发纤维维(胆碱能纤维)经穹
窿、海马伞,终止于海马和齿状回。
•
• ④一侧海马发纤维经同侧海马伞、穹窿 脚,经过海马连合至对侧穹窿脚与海马伞, 终止于对侧海马和齿状回。
储粮昆虫学:储藏物昆虫的分类(五)
第三节 储藏物昆虫的分类
(一)金小峰科Pteromalidae 微小,体长1~3mm,活泼能行走、跳跃及 飞行。大多具金属光泽。头圆形,复眼大;触角 膝状,一般13节,具环状节2~3节,前胸宽短, 常宽于中胸。中胸盾纵沟完整或不完整,并胸腹 节常具侧褶及颈。后足腿节不膨大,胫节末有 1~2个端距。跗节5节。腹部短,没有明显的柄。
第三节 储藏物昆虫的分类
(一)金小峰科Pteromalidae 本科昆虫全世界已记述近500属2000种左右, 多寄生于其他昆虫的幼虫或蛹,但也有极少数种 类寄生植物的种子,生活习性极其复杂。储藏物 中最常见的种类是米象金小蜂Lariophagus distinguendus(Fbrster)(图2-50),可寄生象虫 的幼虫或蛹。
第三节 储藏物昆虫的分类
(三)小茧蜂科 Braconidae 体微小至小型,体长2~12mm,某些雌虫产 卵管长度约等于体长。复眼裸露,单眼3个。后足 腿节简单或加粗,有齿或无齿。翅细,亚缘室2~3 个,肘室2个,中室1个。翅脉有时退化。腹部无 柄、半无柄或有腹柄,圆筒形或卵圆形,第一至 第八或第三至第八腹节可见。
第三节 储藏物昆虫的分类
十、双翅目 Diptera 双翅目为昆虫纲中的大目之一,包括各种蝇、虻、蚊、 蚋等种类,已知种类近12万种。成虫在日间活动者,有访花 果及腐败物质的习性,以花蜜或污物为食;有的种类夜间活 动,吸食其他动物的血液;另一些种类捕食性,取食昆虫或 其他节足动物的幼虫,对控制自然界某些害虫的种群密度起 着重要作用。与储藏物有关的种类属于窗虻科 (Scenopinidae)、瘿蚊科(Cecidomyidae)和酪蝇科 (Piophilidae)。
储藏物昆虫的分类学
第三节 储藏物昆虫的分类
anaerotruncus 筛选 检索
文章题目:深度剖析anaerotruncus:从筛选到检索1. anaerotruncus 的概念和定义anaerotruncus,指的是一种具有厌氧代谢特性的细菌,常见于人体肠道中。
它是肠道微生物群落中的一员,与人体健康密切相关。
在研究肠道菌群多样性和代谢功能的过程中,anaerotruncus 的作用逐渐受到关注。
2. 筛选 anaerotruncus 的方法在研究 anaerotruncus 时,科研人员首先需要进行筛选,以确定其在肠道微生物群落中的存在和丰度。
目前,常用的筛选方法包括16S rRNA基因测序、荧光原位杂交技术等。
通过这些技术,科研人员可以对肠道微生物群落进行深入分析,确定 anaerotruncus 的种群结构和数量。
3. 检索 anaerotruncus 的意义一旦确定了 anaerotruncus 的存在和丰度,科研人员就需要进一步检索其在肠道微生物群落中的作用和功能。
通过对 anaerotruncus 的代谢产物、与其他菌群的相互作用等方面的检索,可以深入了解其与人体健康的关系,为相关疾病的研究提供重要依据。
4. 深度和广度的探讨通过对 anaerotruncus 的筛选和检索进行全面评估,可以看出其在肠道微生态平衡、免疫调节等方面的重要角色。
anaerotruncus 与肥胖、炎症性肠病等疾病的关系也备受关注。
在研究时,还需要考虑到不同环境因素对anaerotruncus 的影响,以及其在不同人群中的分布特点,从而更加深入地探讨其对人体健康的影响。
5. 总结和回顾性内容通过上述对anaerotruncus的筛选和检索的全面评估,可以看出其作为肠道微生物群落中的一员具有重要意义,但在其功能、作用机制、以及与人体健康相关疾病的关系等方面,还有很多待深入研究和探讨的内容。
未来需要进一步开展关于 anaerotruncus 的研究,以更好地认识其与人体健康的关系,并探讨其在相关疾病的治疗和预防中的潜在应用。
医学微生物中的拉丁希腊语汇
引言:医学微生物研究是众多领域中的一个重要分支,它关注着人类健康与疾病的微生物学特性。
在医学微生物的研究中,拉丁希腊语汇起到了关键的作用。
拉丁希腊语是医学专业的基础,许多微生物的命名和描述都基于这一古老语言。
本文将为您介绍医学微生物中常用的拉丁希腊语汇,以加深您对微生物学的了解。
正文概述:一、微生物的分类与命名1.拉丁词根与后缀:拉丁希腊语在微生物分类中的使用具有拉丁希腊词根的微生物名词解析常见的拉丁后缀及其意义2.常见微生物的拉丁希腊名病原微生物的拉丁名解析常见医学微生物的拉丁名及其相关特点3.拉丁希腊语与微生物分类学的关系拉丁希腊语对微生物命名的规范作用微生物分类学的发展与拉丁希腊语的应用二、拉丁希腊语对微生物学研究的影响1.进化与发展拉丁希腊语在微生物进化研究中的应用拉丁希腊语用于描述微生物的进化和分布规律2.药物研究与治疗拉丁希腊语对药物命名的影响拉丁希腊语用于描述微生物药物耐药性与抗生素作用机制3.疾病与诊断拉丁希腊语对疾病命名的相关意义拉丁希腊语用于描述微生物感染与传播的过程4.环境与资源拉丁希腊语对微生物环境适应的揭示拉丁希腊语用于描述海洋和土壤中的微生物资源5.教育与研究拉丁希腊语在微生物学教育中的重要性拉丁希腊语在微生物学研究中的应用价值结论:在医学微生物学的研究中,拉丁希腊语是一门重要的语言。
通过上述内容的详细阐述,我们可以看出拉丁希腊语在微生物分类、命名、进化和发展、药物研究与治疗、疾病诊断、环境与资源以及教育与研究等方面的应用价值。
深入了解和掌握这门语言,对于从事医学微生物学的研究者和医务工作者来说至关重要。
同时,拉丁希腊语也体现了微生物学的深厚历史和文化底蕴,反映了人类对微观世界的探索和认知,对于推动微生物学的发展起到了积极的促进作用。
贝类奥尔森派琴虫可视化LAMP检测技术的建立与应用
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tau蛋白轴突内运输
3817 Research Article3818tau constructs linked to fragments derived from the 3′ UTR region of tau mRNA were employed. The minimal tau ALS, which is required and sufficient for axonal localization, was identified. This region includes the stabilization sequence of tau mRNA, which binds to the HuD stabilization protein (Aranda-Abreu et al., 1999; Good, 1997). We observed that tau mRNA was non-randomly distributed in the cells; instead it was localized as discrete granules along the axon and in the growth cone and it colocalized on the MTs with ribosomal proteins, which indicated the presence of protein synthesis machinery in the axon (Aronov et al., 2001). RNA granules have been observed in fibroblasts, oligodendrocytes and primary neuronal cell cultures, suggesting that they may consist of RNA-protein complexes that contribute to the formation of cellular microdomains (Bassell et al., 1999; St Johnston, 1995; Wilhelm and Vale, 1993).In this study, we identify HuD and KIF3A as components of the tau RNP granules in the neuronal axon and growth cone. We suggest that these proteins are involved in the movement of the granules and the attachment of the granules to the MT tracks. Following targeting of tau mRNA to the axon, we observed local translation of GFP-tau protein as ‘hot spots’along the axon and in the growth cone, thus indicating simultaneous local translation, coinciding with tau mRNA localization in the distal processes. To our knowledge, this is the first axon-targeted mRNA to be shown to be locally translated in the axon.Materials and MethodsP19 stable cell culturesA P19 stable cell line expressing a GFP-tau coding-ALS construct (axonal localization signal of 240 bp from tau 3′ UTR 2529-2760) was used in this study (Aronov et al., 2001).P19 cells were grown and differentiated as previously described (Falconer et al., 1992) in MEM medium containing 10% fetal calf serum, 100 units/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine in an incubator with 5% CO2.In situ hybridization analysis, RNA probes and immunohistochemical stainingDifferentiated P19 cells were fixed with 4% paraformaldehyde in the presence of 4% sucrose (Litman et al., 1993). GFP (452 bp), tubulin (400 bp) and tau (400 bp) single-stranded RNA probes (Aronov et al., 2001) were synthesized in the sense and antisense orientations, using the appropriate polymerase (T3 or T7 RNA polymerase) in the presence of digoxigenin UTP (RNA transcription kit, Boehringer Mannheim Biochemicals). In situ hybridization was performed as previously described (Aranda-Abreu et al., 1999). For visualization of the in situ hybridization signals together with immunostaining, the slides were incubated overnight at 4°C with HRP-conjugated monoclonal anti-Dig conjugated to CY5 (1:500) (Jackson ImmunoResearch) together with the specific antibodies: monoclonal tubulin (1:200) (Sigma), KIF3A (1:30) monoclonal antibodies, KIF 1A(1:100) polyclonal [kindly provided by N. Hirokawa, Japan (Kondo et al., 1994)], and HuD (1:1000) monoclonal antibodies 16A11 or human purified HuD antibodies [kindly provided by H. Furneaux, USA (Marusich et al., 1994)]. The slides were washed with PBS and then incubated for 2 hours at room temperature with the appropriate secondary antibodies, that is, goat anti-mouse (1:500) (Jackson), goat anti-human (Jackson), and goat anti-rabbit (Jackson) for the polyclonal and monoclonal antibodies, respectively. The coverslips were mounted with mowoil and visualized with an LSM confocal laser scanning imaging system equipped with a 40×objective, using the following laser wavelengths: for GFP, excitation 488 nm, emission 505-550 nm; for CY3-goat anti human, excitation 545 nm, emission 560−580 nm; and for tau mRNA labeled with CY5-digoxigenin, excitation 650 nm, emission 680 nm. For detection of endogenous tau mRNA, an RNA probe of tau repeats was used (Fig.3) (Aranda-Abreu et al., 1999). KIF3A was detected by FITC-goat anti-mouse secondary antibodies, excitation 488 nm, emission 505-550 nm. Control experiments for in situ hybridization showed no background with the sense probe. In analysis of the fluorescence staining, no signal was observed in the absence of primary antibodies, and no penetration of signals between specific laser was detected. Image analysisImage analysis of in situ hybridization experiments was performed using a Zeiss LSM confocal microscope with LSM 510 software equipped with a 40×(1.0 numerical aperture) and 100×(1.4 numerical aperture) oil immersion lenses. The axon showing the in situ hybridization signal was divided into 20 µm segments, and the number of granules was counted and their size measured. For colocalization experiments, images of the same axon region observed by the GFP or rhodamine specific filter set and marked by the program coordinate system were analyzed. To determine whether a tau mRNA hybridization signal colocalized with HuD and KIF3A proteins, the coordinates of the particle and the proteins were compared. Only when the coordinates were identical were the particles considered to colocalize.For quantitative analysis, 25-45cells were analyzed on one coverslip for each experiment.Experiments were done with three coverslips for each variable,and each experiment was repeated at least three times. The data were analyzed by ANOV A and Student’s t test, and statistical significance was determined for the experimental conditions.Protein synthesis inhibition in P19 cellsDifferentiated P19 cells (8-10 days) were treated with 50 µg/ml emetine (Sigma), a protein translation inhibitor, for 3 to 4 hours. At the end of the treatment, the medium was replaced with fresh medium, and the cells were left to recover for the specified time periods. Cells were visualized by confocal microscopy with GFP-adapted filters as described above. The quantitative analysis of mRNA and protein localization within the axon was measured by fluorescence (pixel) intensities. Regions of interest (ROIs) were marked, and the mean fluorescence intensity per pixel (±s.e.m.) was measured over time, relative to t0(Job and Lagnado, 1998). Care was taken to minimize ‘background pixels’ within ROIs, and background fluorescence was subtracted from the mean fluorescence. The data were analyzed by ANOV A and Student’s t-test to find the statistical significance under the various experimental conditions. The data were displayed by using Adobe Systems (Mountain View, CA).To monitor tau protein synthesis during the time-lapse experiments, cells grown on coverslips were transferred to growth chambers maintained at 37°C on a heated stage. The cell medium was changed to Ham’s F12 medium without phenol-red indicator. Fluorescence images were captured at the indicated time points with a cooled CCD camera and processed with LSM 510 software (Zeiss Germany). To minimize photobleaching and phototoxicity of the living cells, a computer-driven automatic shutter was used to achieve minimum illumination.Antisense oligodeoxynucleotide (ODN) treatment of differentiated P19 cellsDifferentiated P19 cells (8-10 days) were treated for 30 hours with 50µM unmodified sense or antisense KIF3A ODN, essentially asJournal of Cell Science 115 (19)3819T au RNP granules and synthesis in axonspreviously described (Aranda-Abreu et al., 1999). The medium of the treated cells was replaced every 10 hours, with fresh medium and antisense ODN. The sequence of the antisense KIF3A ODN was 5′-TCCTACTTATTGATCGGCAT-3′(1-20)(Kondo et al., 1994). No significant homology was found between the antisense KIF3A ODN and other sequences in the database. The morphological appearance of the treated P19cells was observed by light microscopy. Following removal of the antisense ODN and replacement with fresh medium, the cells resumed normal morphology.Immunoblot analysis of P19 protein extractsProteins were extracted from P19 cells (treated with KIF3A sense or antisense ODN) in 1 volume of lysis buffer consisting of 50 mM Tris pH 8.5, 1% Triton X-100, 5 mM EDTA, 0.15M NaCl and 50 µg/ml PMSF. Cell debris was removed by centrifugation for 10 minutes at 16,000 g at 4°C. Protein samples (25 µg) were resolved by sodium dodecyl sulfate (SDS) polyacrylamide-gel electrophoresis, transferred to nitrocellulose filters and reacted with the specified monoclonal antibodies at 4°C for 16 hours. Following incubation for 1 hour at room temperature with HRP anti-mouse secondary antibodies (Jackson ImmunoResearch), the blots were developed using the ECL chemiluminescence technique.Immunoprecipitation analysis of P19 cell extractsThe preparation of cell extracts and immunoprecipitations was performed as previously described (Aranda-Abreu et al.,1999). Anti-HuD sera, anti-KIF3A and anti-βtubulin (Sigma)were added and the mixture incubated for hour at 4°C,followed by incubation with protein A-sepharose (Pharmacia,10% final concentration). The immunocomplex was precipitated by centrifugation, washed, analyzed by SDS-PAGE and processed as already described.ResultsIdentification of HuD and kinesin proteins in the tau mRNA granulesIn a previous study using differentiated PC12 cells, we detected an in vivo association between HuD RBP and tau mRNA (Aranda-Abreu et al., 1999). This binding stabilizes the tau message and is dependent on an AU-rich element located in the tau ALS. The binding of the HuD protein is mediated by association with the MTs,which may anchor the message prior to its local translation (Aronov et al., 2001).To further characterize tau mRNA granules in differentiated P19 cells, we tested the colocalization of tau mRNA and HuD protein by in situ hybridization and immunostaining using confocal microscopy (Fig. 1Aa).GFP-tau protein was seen in the cell body and axon (Fig.1Ab), which is similar to the location of transfected GFP-tau mRNA detected using the GFP probe in differentiated cell lines (Fig. 1Ad).Immunohistochemical analysis using HuD-specific antibodies demonstrated that HuD protein is highly enriched in the neuronal cell body and in the axon of differentiated P19 cells, whereas only a faint staining was observed in the dendrites (Fig. 1Ac). The merged image, presented at a higher magnification (×2500), of tau mRNA (red signal) and HuD protein (cyan signal)Fig. 1.HuD protein colocalizes with tau mRNA in RNP granules.(A)Confocal image analysis of HuD protein localization in differentiated P19 cells. (a) Phase image of a differentiated P19 cell. (b) Expression of GFP-tau protein. (c) Staining with HuD antibodies. (d) In situhybridization analysis with the GFP probe (which detects the transfected tau mRNA). (e) Merged confocal image of b, c and d (×2500magnification). The solid arrowheads denote axons; empty arrowheads denote dendrites; and small solid curved arrowheads denote neuronal cell bodies. Bar, 10 µm. (B)Confocal merged image of a growth cone. An enlarged merged image from the growth cone of the cell shown in A. The merged staining includes the GFP-tau fluorescence (green), HuDimmunostaining (cyan) and GFP in situ hybridization (red) to yield a white area of colocalization. Aggregation of tau granules is marked by a solid white arrowhead; free HuD protein (cyan) is marked by an asterisk. Bar,1µm (×20000 magnification).3820coincided with that of GFP-tagged tau protein (green signal),all of which overlapped to yield white-pink granules, which are distributed along the axon (filled arrowhead) (Fig. 1Ae).A merged image at high magnification of the axon and the growth cone is shown in Fig. 1B. The size of the small granules is between 0.3-0.5 µm (20 granules were measured in each axon of 18 different cells, P <0.01), and the size of the larger granules is between 0.7-1.5 µm (10 granules were measured in each axon of 20 different cells). The variation in the size of these granules, which include tau mRNA, suggests the presence of two size groups, which may represent the small granules and aggregates of granules attached to the MTs that were previously detected in neuronal and oligodendrocyte cells (Knowles et al., 1996; Bassel et al., 1998; Ainger et al., 1997).The previously described small granules contain other mRNAs and exhibit dynamic behavior, whereas the larger granules were hypothesized to represent the translationally inhibited granules that were recently identified in neuronal primary cultures (Krichevsky and Kosik, 2001).Free HuD protein (Fig. 1B), seen as cyan dots (marked by an asterisk), and not associated with MTs, may represent an oligomeric structure consisting of the protein alone or bound to other mRNAs that are not detectable by the GFP in situ hybridization probe (Tenenbaum et al., 2000). Similar aggregates have been observed in embryonic cortical neuron cell cultures and HeLa cells stained with HelN1 and HuR antibodies, both of which are HuD homologues (Brennan et al.,2000; Gao and Keene, 1996).The kinesin motor family is involved in the directional movement of RNP granules along the MTs (Carson et al.,1997; Severt et al., 1999).To determine whether kinesin expression affects axon outgrowth and tau mRNA sorting in differentiated P19 cells, we focused on one member of the kinesin motor family, KIF3A, which is localized primarily in the axons and is involved in anterograde movement (Kondo et al., 1994). Our experimental data showed increased levels of KIF3A protein in the differentiated P19 cells, as detected by KIF3A-specific antibodies (data not shown). Immunostaining of differentiated P19 cells with KIF3A-specific antibodies showed intense staining in the cell body and axon and fainter staining in the dendrites. The staining of kinesin protein showed colocalization with the tau mRNA (Fig. 2Ad), which was detected as pink granules in the axon of the enlarged confocal image, while no colocalization was seen in the dendrites (Fig. 2Ae). A high magnification image of the axon and growth cone (Fig. 2B) shows granules that contain tau mRNA, kinesin and GFP-tau protein distributed along the axon and in the growth cone (white-pink granules). Free kinesin protein, which did not colocalize with either tau mRNA or GFP-tau protein, is seen as cyan dots along the axon.The above experiments indicated that tau RNP granules in the P19 cell line expressing GFP-tau ALS (Aronov et al., 2001)included HuD and KIF3A proteins and prompted us to test the colocalization of both proteins in the tau mRNA granule. For that experiment, non-transfected cells were used (owing to technical limitations of visualizing more than three chromophores). Endogenous tau mRNA was visualized by in situ hybridization using an RNA probe derived from the region of tau 4 repeats (Fig. 3b) together with HuD (Fig. 3c) and KIF3A (Fig. 3d) antibodies. Colocalization of KIF3A, HuD and endogenous tau mRNA is shown in Fig. 3e and isJournal of Cell Science 115 (19)Fig. 2.KIF3A protein colocalizes with tau mRNA in RNP granules.(A)Confocal image analysis of KIF3A protein localization in differentiated P19 cells. (a) Phase image of differentiated P19 cell.(b) Expression of GFP-tau protein. (c) Staining with KIF3A antibodies. (d) In situ hybridization analysis with the GFP probe (which detects the transfected tau mRNA). (e) Merged confocal image of b, c and d (×2500 magnification). The solid arrowheads denote axons, empty arrowheads denote dendrites and small solid curved arrowheads denote neuronal cell bodies. Bar, 10 µm.(B)Confocal merged image of a growth cone. The enlarged merged image from the cell shown in A. The merged staining includes the GFP-tau fluorescence (green), KIF3A immunostaining (cyan) and GFP in situ hybridization (red), yielding a white colocalization.Aggregation of tau granules is marked by a solid arrowhead. A non-localized KIF3A signal (cyan) is marked by an asterisk. Bar, 1 µm (×20000 magnification).3821T au RNP granules and synthesis in axonsmagnified twice compared with the magnifications shown in Fig. 1B or Fig. 2B for HuD and KIF3A, respectively. HuD andKIF3A proteins, which colocalize with tau mRNA, are present in the axon and growth cone. Quantitative analysis was performed on 10-12 fields from four separate experiments,analyzing the size and proportion of tau mRNA granules that colocalize with KIF3A or HuD proteins. The size of the granules ranged between 0.5-0.7 µm (P <0.01), and 57.5% of the granules exhibited tau mRNA and HuD (P <0.01), whereas 15-22% of the granules exhibited tau mRNA, HuD and KIF3A (P <0.001). These results demonstrated that only a fraction of tau mRNA granules colocalized with the KIF3A motor protein;this fraction may represent the granules that are capable of moving in the anterograde direction. Previous experiments using live cells imaging have shown that mRNA granules may move in both the retrograde and anterograde directions (Rook et al., 2000; Zhang et al., 2001). The higher proportion of tau mRNA granules colocalized with HuD protein may indicate the multiplicity of functions that HuD is involved in; thus it is in the core of the granule. In the analysis obtained from this experiment, granules containing endogenous tau were visualized. These comprise about 15-20% of total axonal granules, as tested by uniform SYTO14 staining (S.A. and I.G.,unpublished). Taken together, these results suggest that both HuD and KIF3A are components of the tau granules.The effect of KIF3A antisense ODN treatment on differentiated P19 cellsThe effect of KIF3A antisense ODN treatment on the distribution of tau mRNA was tested in differentiated P19 cells.Differentiated P19 cells were treated with ODN for a period of up to 30 hours, and the effect of the treatment on the axons is shown in the field view (Fig. 4A) and at the single cell level (Fig. 5). The treatment with kinesin antisense ODN caused axonal retraction, whereas in control sense ODN-treated cellsFig. 3.HuD, KIF3A and tau mRNA colocalize in RNP granules.(a)Phase image: the growth cone is shown on the right side of the panel. Colocalization of tau mRNA (b, red), HuD protein (c, green)and KIF3A (d, cyan) to yield the merged image presented in e of the axon and growth cone of differentiated P19 cells. The curvedarrowhead denotes colocalization of the three components (white).The asterisk denotes colocalization of HuD and KIF (light green).The straight arrowhead denotes colocalization of HuD and tau mRNA (yellow). Bar, 1 µm (×40,000 magnification).Fig. 4.(A) A field view of differentiated P19 cells treated with KIF3A antisense ODN, analyzed using a confocal micoscope. (a,b)Control untreated cells; (c,d) treated cells; (a,c) GFP-tau fluorescence. (b,d) KIF3A protein localization visualized byimmunohistochemical staining with KIF3A antibodies. Bar, 20 µm.(B) Immunoblot analysis of cell extracts prepared from control-differentiated P19 cells or from cells treated with KIF3A antisense ODN and KIF3A sense ODN. The blots were reacted with KIF3A,KIF1A, tau, tubulin, MAP2, synaptophysin and neurofilament antibodies. (This is a representative blot from three experiments.)3822no axonal retraction was observed,and both GFP-tau and kinesin wereobserved throughout the axons andreached the growth cones (Fig. 4Aa,b).(Growth cones are seen in the upperright hand corners and marked by solidarrowheads; Fig. 4.) The average axonlength of antisense-treated P19 cellsdropped by 3.5-fold to 91.5 µm±13.91,whereas sense-treated cells exhibitedaxon lengths of 346.18 µm±38.15,P<0.05 (20-30 cells were measuredper treatment, in four separateexperiments).The level of KIF3A protein wasmeasured in total cell extracts preparedfrom untreated differentiated P19 cellsand from cells treated with KIF3Aantisense or sense ODN. The results ofthe western blot analysis (Fig. 4B)demonstrate the effectiveness of theantisense ODN treatment. The level ofKIF3A protein was 35% of controlcells, whereas no effect was observedfollowing treatment with KIF3Asense ODN. The level of KIF1A,neurofilament synaptophysin andMAP2 proteins remained unaffected,suggesting that the treatment is specificto KIF3A. The levels of endogenoustubulin and tau proteins dropped to 70%and 80%, respectively, which mayreflect the retraction of neurites in thetreated cells.Closer analysis of a single cell treatedwith KIF3A antisense (Fig. 5f-j) orsense (Fig. 5k-o) ODN compared to thecontrol cell (Fig. 5a-e) is shown inFig. 5. Antisense-treated cells haddiminished GFP-tau fluorescent signals(Fig. 5g) and tau mRNA levels (Fig. 5h)as well as axonal retraction. The levelsof GFP-tau protein and tau mRNAlevels reached 50% and 55%,respectively, of sense-treated cells (Fig.5l,m), measured in pixels per unit area,as described in Materials and Methods.Measurements were taken from fourdifferent experiments, and 30 cells wereanalyzed. The in situ hybridizationanalysis with the GFP probe revealedthat the tau mRNA signal is lower in theaxon and in the cell body (Fig. 5h)compared with control cells (Fig. 5c).Moreover, its distribution is altered andis seen in the cell body concentratedclose to the outer membrane (Fig. 5h).The perturbed distribution of tau mRNAcould have been caused by theinhibition of mRNA transport from thecell body to the axon. Previously, we Journal of Cell Science 115 (19)Fig. 5.Confocal image analysis of a single differentiated P19 cell treated with KIF3Aantisense ODN. (a-e) Differentiated untreated P19 cells. (f-j) KIF3A antisese ODN-treatedcell. (k-o) KIF3A sense ODN-treated cell. (a,f,k) Localization of KIF3A protein, asvisualized by staining with anti-KIF3A antibodies. (b,g,l) Expression of GFP-tau protein.(c,h,m) In situ hybridization with the GFP probe (which detects the transfected taumRNA). (d,i,n) Localization of tubulin protein, as visualized by staining with tubulinantibodies. (e,j,o) In situ hybridization with tubulin. Solid arrowheads denote axons, emptyarrowheads denote dendrites and small solid curved arrowheads denote neuronal cellbodies. Bar, 10 µm.3823T au RNP granules and synthesis in axonsFig. 6.Co-immunoprecipitation of HuD, KIF3A and tubulin fromof HuDsuggesting an interactionbetweentheperformed with anti-tubulinantibodies (IP Tub) and tested forthe presence of kinesin, a strongsignal was observed, suggesting thata strong affinity exists betweenKIF3A and the tubulin. Thissupports previous studies thatdemonstrated kinesin movement onFig. 7.differentiated living P19 cell linestreated with emetine, a protein-synthesis inhibitor. (Aa,b) A controldifferentiated cell. The insert showsthe enlargement of the growth coneregion. (c,d) GFP-tau proteinfluorescence in an emetine-treated cell.(e,f) GFP-tau protein fluorescence in acell following a recovery period of 3hours in fresh media. (B) Tau mRNAis found in emitine treated cells.(a)shows no GFP fluorescence (b). (c) Insitu hybridization with GFP probe.Solid arrowheads denote axons, emptyarrowheads denote dendrites and smallsolid curved arrowheads denoteneuronal cell bodies. Bar, 103824microtubules (Hirokawa, 1998). The cumulative results suggestthat an association exists between HuD and kinesin proteins.Furthermore, they indicate that the complex associates with MTs. Our previous results, demonstrating an association between tau mRNA, HuD protein and MTs, together with the results presented in this study, add KIF3A to the protein components present in the tau RNP granules.Local translation of tau protein in the axons of living P19neuronal cellsAccording to the multistep localization hypothesis, following the targeting of the mRNAs, local translation should occur,which may respond to external signals (Wilhelm and Vale,1993; Zhang et al., 2001). In a previous study we demonstrated the presence of GFP-tau mRNA, tau proteins and ribosomes in the axons of differentiated P19 cells (Aronov et al., 2001). To examine the local translation of GFP-tau protein, emetine, an inhibitor of the translocation step in protein synthesis, was added to the differentiated P19 cells stably expressing a GFP-tau-coding construct, which included the ALS at its 3′ UTR (Fig. 7). Application of emetine for 3-4 hours caused the disappearance of the GFP-tau fluorescent signal in the cell body and axon (Fig. 7Ac, Fig. 7Ad). After a recovery period of 3 to 4 hours, GFP-tau protein fluorescence appeared as ‘hot spots’ along the axon and in the growth cone (Fig. 7Ae, Fig.7Af). After 3 hours of recovery, the intensity ratio, expressed as pixels per unit area and measured in the cell body and the axon, was 1:2.5. The quantitative analysis was based on a total of 18 to 30 cells measured at each time point in four separate experiments.To exclude the possibility that the GFP-tau protein observed in the axon originated from transport out of the cell body,time-course analysis of the local synthesis was visualized (Fig.8). GFP-tau protein was initially observed along the axon after 1.5 hours of recovery, whereas no signal was yet seen in the cell body. The signal was observed in the cell body only 2.5hours later. (The cell bodies are circled in Fig. 8.) Moreover,the intensity of the GFP-tau ‘hot spots’ was stronger in the axon than in the cell body. The increase in intensity of the axonal ‘hot spots’, measured as fluorescence pixels per area unit, was highest (3.5 fold) during the first 30 minutes of observation. From 1.5-2 hours and 2-3 hours of incubation,the intensity increased by two- and 1.3-fold, respectively.Recently, using a similar approach in transiently transfected hippocampal neurons, local translation of dendritically targeted GFP-reporter protein was observed. It was suggested that the rate of GFP translation was exponential in the dendrites but linear in the cell bodies (Job and Eberwine,2001).Since emetine affects protein synthesis, we checked whether tau mRNA was still present in cells after 3 hours of treatment with emetine (Fig. 7B). Tau mRNA was observed in the cell body and axon (Fig. 7Bc) when no GFP-tau signal was visible (Fig. 7Bb), which is similar to the localization of tau mRNA in differentiated cells (Fig. 1c).On the basis of the time frame during which the GFP-tau ‘hot spots’ were visualized along the axon and no signal was observed in the cell body, these observations are consistent with local protein synthesis in the axon being dependent on transported tau mRNA.Journal of Cell Science 115 (19)Fig. 8. Local GFP tau synthesis in the axon of P19 differentiated cell during the recovery from emetine treatment. (a) Phase image. (b,e)Recovery periods of 1.5, 2, 2.5 and 3 hours. The cell bodies are circled. Bar, 10 µm.3825 T au RNP granules and synthesis in axonsDiscussionIn this study, employing differentiated living P19 cells, we identified two proteins contained in tau mRNA granules that are highly concentrated in the cell body, axons and growth cones. The advantage of the P19 system is that it is not transiently transfected and therefore does not overexpress the studied mRNA. As such, it may mimic the regulation of endogenous tau mRNA (Aronov et al., 2001). The identity of these granules was indicated by their specific in situ hybridization with tau probe, immunostaining with HuD and KIF3A antibodies and colocalization with MTs (Figs 1 and 2). These results are in agreement with our previous demonstration of in vivo binding of HuD to a specific sequence located in the 3′ UTR of tau mRNA and its colocalization with the MT system (Aranda-Abreu et al., 1999; Litman et al., 1994). The identification of HuD protein as a component of the tau RNP granule suggests multiple functions for this protein. It can act as an mRNA-stabilizing protein (Aranda-Abreu et al., 1999) and can also serve as a linker protein to the MT tracks going down the axon. Interestingly, recent experiments have suggested that Hel-N1, a homologue of HuD, controls the translation of neurofilament M mRNA in human embryonic terato carcinoma cells through the recruitment of neurofilament mRNA into the heavy polysome fraction; this recruitment is dependent on sequences located in the 3′ UTR of neurofilament M mRNA (Antic et al., 1999). This activity fits with the recent data demonstrating that RNA granules include mRNAs and clusters of ribosomes in a non-translated form, which upon activation move to the polysome fraction (Krichevsky and Kosik, 2001). The Elav protein family was originally identified in Drosophila as being involved in neurogenesis and thus may function in an MT-dependent manner (Antic and Keene, 1998; Aranda-Abreu et al., 1999; Aronov et al., 2001). As we demonstrated that HuD protein binds directly to a specific cis signal located in the tau 3′ UTR, this may suggest that HuD binding is among the initial events in mRNA granule assembly and is also involved in mRNA shuttling from the nucleus to the cytoplasm (Campos et al., 1985; Ma et al., 1997). Moreover, recent data demonstrating that the Elav protein family binds to additional protein ligands, which do not bind directly to the targeted mRNA, adds additional insight into their function in diverse signaling cascades in vivo (Brennan et al., 2000). Studies on β-actin axonal localization in neuronal primary cultures have demonstrated that the accumulation of β-actin mRNA and protein in the axonal growth cones can be induced by dibutyryl cAMP treatment or stimulated by application of neurotrophins. This axonal localization depends on the formation of an RNP complex between β-actin mRNA with two proteins, ZBP1 and ZBP2, that have been identified through their specific binding to the axonal localization sequence within the 3′ UTR of β-actin mRNA. Both proteins have a role in β-actin mRNA localization, and ZPB2 most probably shuttles between the nucleus and the cytoplasm (Bassell et al., 1998; Gu et al., 2002; Zhang et al., 1999; Zhang et al., 2001).Deciphering which of the motor proteins, belonging to the kinesin or dynein family, translocate the granules along their specific tracks – either MTs or microfilaments – is a crucial and unresolved issue (Hirokawa, 1998; Kikkawa et al., 2001; Schnapp, 1999). The specific interaction between these motor proteins and mRNAs may explain the asymmetric mRNA localization within the neuronal microdomains. These motor proteins use the cytoskeleton for active subcellular mRNA localization. Using HuD antibodies as bait for co-immunoprecipitation experiments, we found that KIF3A is present in the complex that associates with MTs (Fig. 6). Moreover, as shown previously by RT-PCR analysis, tau mRNA was identified in the pellet immunoprecipated by HuD antibodies (Aranda-Abreu et al., 1999). Similarly, tau-mRNA was identified in the KIF3A immunoprecipitate (data not shown). KIF3A belongs to the kinesin superfamily and has been characterized as an MT-based anterograde motor enriched in neuronal axons (Hirokawa, 1998; Hirokawa, 2000; Kondo et al., 1994). A complex between the tau 3′ ALS region and a protein of a similar size to KIF3A was previously detected by a UV crosslinking assay using brain or neuronal cell extracts (Behar et al., 1995).To examine the possible functional association of tau with kinesin in vivo, we tested the effect of treatment with antisense ODN specific for KIF3A on neuronally differentiated P19 cells. The treatment caused retraction of neurites, with a more severe effect on the axons, where a higher concentration of KIF3A protein was detected. This treatment caused a specific decrease of KIF3A protein level in the cell, whereas the level of KIF1A protein was not affected. There was a concomitant decrease in tau mRNA levels, as tested by in situ hybridization, and a change in its distribution in the cell body, probably because of the inhibition of tau mRNA targeting. Previous studies have shown that translocation of myelin basic protein in oligodendrocytes requires MTs and kinesin (Carson et al., 1997; Carson et al., 1998). In another study, inhibition of kinesin heavy chain expression by ODN treatment of neonatal rat hippocampal neurons inhibited dendritic localization of α-CAMKII. Although we cannot disprove completely the idea that the effects on tau mRNA and protein localization are caused by the secondary effect of axonal retraction, this possibility is less favorable since our preliminary results using SYTO14 staining of total granules (Knowles et al., 1996) show that following anti-kinesin ODN treatment, the velocity of the granules is reduced, whereas their density per unit length remains the same (S.A. and I.G., unpublished). Therefore, the current results and the previous observations in oligodendrocytes and neurons indicate the function of kinesin motor proteins in targeting of mRNA granules to specific cellular microdomains (Carson et al., 1997; Severt et al., 1999). The interaction of the kinesin proteins with MTs may link the granules to the MT-assisted ATP movement in the plus-end-oriented MTs present in the axons (Kikkawa et al., 2001). Previous studies have shown that transport by the KIF3A complex exhibits a velocity of 0.3 µm/second and that its association with vesicles in rat axons is involved in fast axonal transport (Hirokawa, 2000). This may imply its involvement in axonal sprouting events by supplying the required material (Takeda et al., 2000).We observed two major classes of granules in our studies, which may represent small granules and aggregates of granules, both of which are mostly concentrated toward the lower region of the axon and in the growth cones. The calculated size of the observed small granules was between 0.3 and 0.5 µm in diameter, similar to the size observed in neuronal cells and in lamellopodia of fibroblasts (Kohrmann et al., 1999; Krichevsky and Kosik, 2001; Latham et al., 1994). These。
常用物种拉丁文名称
Ciona intestinalis (sea squirt,海胆)
Anopheles gambiae(malaria mosquito,按蚊)
anaerotruncus 筛选 检索
anaerotruncus 筛选检索摘要:1.简介2.anaerotruncus的定义与作用3.anaerotruncus筛选与检索的方法4.我国在anaerotruncus研究方面的进展5.总结与展望正文:1.简介本文将介绍anaerotruncus的概念,以及它在筛选和检索过程中的应用。
anaerotruncus是一种微生物,具有较强的降解有机污染物的能力。
在环境保护和污染治理领域,anaerotruncus的筛选和检索具有重要意义。
2.anaerotruncus的定义与作用anaerotruncus是一种厌氧短杆菌,属于革兰氏阴性菌。
它可以分解有机污染物,如石油、化工产品和药物残留等。
anaerotruncus在环境保护领域具有广泛的应用,如废水处理、土壤修复等。
3.anaerotruncus筛选与检索的方法(1)从污染环境中筛选anaerotruncus(2)基于16S rRNA基因序列进行anaerotruncus的鉴定和分类(3)利用生物信息学方法进行anaerotruncus的基因组分析和功能预测4.我国在anaerotruncus研究方面的进展近年来,我国在anaerotruncus研究方面取得了显著进展。
不仅在anaerotruncus的筛选和检索技术上有所突破,还在anaerotruncus的应用研究方面取得了重要成果。
这些成果为我国环境保护和污染治理提供了有力支持。
5.总结与展望anaerotruncus在环境保护和污染治理领域具有广泛的应用前景。
随着科研技术的不断发展,相信在不久的将来,anaerotruncus将在更多领域发挥重要作用。
瓦里亚什科分类方法-概述说明以及解释
瓦里亚什科分类方法-概述说明以及解释1.引言1.1 概述瓦里亚什科分类方法是一种用于对物种进行分类和命名的系统。
它是由俄罗斯植物学家维克托·瓦里亚什科于20世纪中期提出的,是基于生物分类学的一种重要方法。
瓦里亚什科分类方法通过对物种的形态、生态学和遗传学等特征进行综合分析,将物种分类归属到适当的分类单元中,从而便于科学家对生物多样性进行研究和保护。
瓦里亚什科分类方法在生物分类学领域具有重要的意义,不仅可以帮助科学家对物种进行准确的分类和命名,还可以为生物多样性保护和资源利用提供科学依据。
本文将从瓦里亚什科分类方法的原理、应用和优势等方面进行详细介绍,旨在展示其在生物分类学中的重要性和价值。
1.2 文章结构文章结构部分主要介绍了整篇文章的组织架构,为读者提供了整体内容的框架和引导,让读者更好地理解文章内容。
文章结构部分包括对引言、正文和结论部分的简要介绍,让读者对整篇文章的主要内容有一个大致的了解。
在本文中,文章结构部分会介绍引言、正文和结论三个主要部分的内容概要。
引言部分会概述瓦里亚什科分类方法的背景和重要性,正文部分将详细介绍瓦里亚什科分类方法的基本原理、应用领域和优势,结论部分将总结瓦里亚什科分类方法的重要性,并展望未来的发展方向。
通过文章结构部分的介绍,读者可以清晰地了解到整篇文章的组织结构和主要内容,使其更容易理解文章的核心思想和观点。
1.3 目的瓦里亚什科分类方法是一种对数据进行分类和组织的重要工具。
本文的目的是深入探讨瓦里亚什科分类方法的原理和应用,帮助读者更好地理解和应用这一方法。
通过分析研究,我们可以了解瓦里亚什科分类方法在不同领域的应用情况,揭示其在数据处理和分析中的优势和局限性,从而为未来研究提供参考和启示。
同时,通过对瓦里亚什科分类方法的研究,我们也可以为推动数据科学和机器学习技术的发展做出贡献,促进相关领域的进步和创新。
因此,本文的目的是系统探讨瓦里亚什科分类方法的相关内容,为读者提供全面的理论基础和实践指导,促进这一方法的更广泛应用和深入研究。
UNOCYGNAEUSandOTTOSALOMONjyufi
Tapani KananojaLETTERS OF UNO CYGNAEUS AND OTTO SALOMONJyväskylä the 22nd of June 1877 - 1st of January 1887The following tests are translations and commentary of letters between UnoCygnaeus and Otto Salomon kept in Helsinki University library. The language of theletters was originally Swedish. The letters have been used in order to show thestandpoints of Cygnaeus and Salomon in development of handicraft education butthey are the only ones, which have been preserved of the correspondence between thetwo gentlemen.The letters of Uno Cygnaeus (1810 - 1888) have been handled by historians of education before. Cygnaeus had a large correspondence because of his work, travels and international relations but also with his former students who were teachers all around the country and started the new school system, which he had founded.The letters between the two Nordic handicraft (‘slöjd’; ‘sloyd’) development authorities, Uno Cygnaeus, a Finn, and Otto Salomon (1849 - 1907), a Swede, have not generally been known in Finland by the handicraft education researchers who mostly have quoted the secondary references only. The thorough history and analysis of Otto Salomon written by Hans Thorbjörnsson (1990), also lacks the letters from Uno Cygnaeus to Otto Salomon. This is because in the 1930s Aukusti Salo, professor of education in Helsinki University, sent a research assistant to collect the letters and bring them back to Finland in order to be preserved here; most of the the letters also were translated in Finnish. Another researcher, Jussila, later on catalogued the letters.The original letters have been kept in the archives of the library of the Faculty of Education in Helsinki University, but researched, as far as it is known, mostly only by the general education historians, eg. Gladh (1968), Halila (1949), Jussila (1968; 1974), Ottelin (1934) and Nurmi (1988). Some handicraft educationalists have also done it, e.g. Laurila (1912) and Harni (1951).Uno Cygnaeus is mentioned in the international educational literature as the first educator in the world who introduced handicrafts to school as a compulsory subject among other researchers by Allingbjerg (1983), Bennet (1937), Brubacher (1966), Grue-Sørensen (1961), Kaiser (1974), Myhre (1985), Olson (1963), Pabst (1907), Raapke (1995), Reincke (1995), Tsiantis (1989), Whittaker (1964), Wilkening (1970).In Finnish Handicraft Education doctoral level research Cygnaeus has been handled at least by Anttila (1982), Autio (1997), Kananoja (1989), Kantola (1997) and Suojanen (1991); most of them, however, only mentioning him as a second hand reference.Most of the letters found now are written in 1881 - 2. There are some years during 1877 - 87, when no letters have been exchanged, and years, when Cygnaeus was continuously active, but did not get any answer from Salomon. Why, can partially be found out in the texts; in some cases because of sickness, sometimes because of disagreements about the ideas. However, along the years the ideological disagreements were smoothly agreed - or at least forgiven.At the time of the letters Uno Cygnaeus was from 67 to 77 years of age. Otto Salomon was younger, from 28 to 38 years. The old age of UC is seen somewhere in the texts in repeating the same issues, and sometimes the meaning of the sentences also disappear in the nice words.Both of the gentlemen were enthusiasts for practical education. The background of them was, however, different. Both of them were intellectually active, having studied education quite individually. OS lived in Nääs, South of Sweden and was trained to be a gardener. UC was a priest and worked as a priest and teacher in St. Petersburg in the Finnish church and in Alaska in an immigration society for years. OS was financed by his relatives to do the work he chose; UC was hired by the government - actually by the Russian Czar - to collect information about the educationproposal for the Government was the basis for the law about folk schools (1866) and for the law about teacher training. In these laws handicrafts (slöjd) was mentioned the first time as a compulsory subject in the world and was given an independent position as a school subject. Later on Cygnaeus was nominated in The National Board of Education as the first and only Chief Inspector responsible for the Finnish language Folk Schools and the respective teacher training institutions, ‘Seminars’.The idea of the Finnish Folk School was revolutionary in many ways. Finland had been under the Swedish regime for about 500 years (up to 1805), and Swedish still was the official language of the country. Later on Finland had an autonomous status in the Russian Empire (1805 - 1917), and the Czar felt sympathy towards the poor, undeveloped country. Also ideas to have the full independence were growing in Finland, and in that sense the national education system teaching the own language was urgently needed. Cygnaeus was later on given the honorary title of ‘the Fat her of the Finnish Folk School’.At the time of the letters Sweden already had the national folk school created by Thorsten Rudensköld (1842), ‘the Father of the Swedish Folk School’, whom UC knew and mentions - and criticises for lack of handicrafts education in a letter in September, 1881. As well UC was disappointed about the reception of his message in the other Nordic Countries. However, his work was admired by many, and especially his former students were true believers in his education ideology.I had the opportunity and an honour to serve as a government official, Chief Inspector, twenty years at the National Board of General Education, at the office, which Uno Cygnaeus created about 100 years earlier. He was my early colleague, the first - and at that time the only - Chief Inspector. As well (Handi)craft Education is a combining factor; he started it; I was working for it for the longest period, twenty years, in the country as the supervisor at the central administration. The more I read about Uno Cygnaeus, the more I admired the unbelievable work he had done. Some of his experiences also resemble mine; e.g. the disappointments about the slow progress of the reforms and the sometimes not so understanding attitudes around, especially, when you retired. During my school years I naturally had heard about him from the teachers like all the Finnish children, as the Father of the Finnish School.I hope that this paper and the celebration we dedicate this week will be understood as an honorary gesture for a Finnish educator. He can still be honoured because he is mentioned in the world history of education as the only Finn. The idea is not to try to re-introduce or admire the old imitative Craft or Industrial Arts Education. However, in order to develop the things further, we have to know the roots. In the rapid development of the modern societies the gap between the real needs for education and the everyday reality in the schools is in danger to grow. Education systems always have been realised on conditions of the older generation. Quite many of the old findings can be misunderstood, if the original connections and emphasis are not sought for.Interesting when reading the letters between Uno Cygnaeus and Otto Salomon, is naturally also the position Uno Cygnaeus had and has globally and how he was understood or respected or not respected by the closest neighbouring countries.In PROSPECTS, a UNESCO document in 1993, Otto Salomon and Thorsten Rudensköld are mentioned as Education Innovators, but not Cygnaeus. This must be understood as a lack of information from Finland but proper activity from Sweden to the document writers; not as the truth about the value or the order of significance of the three innovators.LETTERS IN 1877:(possible underlining in the original letters)During the first year the letters mostly handle the beginning collaboration between the two gentlemen. The first one is a letter from OS to UC (22. June), with which OS after a trip toJyväskylä thanks for the hospitality and asks for the photo of UC to be published in his documents. In his reply (3. July) UC is grateful for the appreciation and emphasises the importance of meeting the ‘…members of the former brother nation with same kind of interests in common important problems li ke handicrafts if the folk school’.OS has during his stay in Finland been satisfied with the ‘spirit and methods, which are applied in the schools’ and hopes that they will be generalised also in Sweden. He invites Finnish teachers to work in Sweden, especially a female teacher to teach in his handicraft school. The first exchange teacher, Ingeborg Lundgren, is hired in 1877. UC tells about being grateful for that and for the writings of OS about handicraft, which he had received and promises to use them as the basis for the becoming Finnish sloyd models.The 28th of October in 1877 the concern of UC is ‘educational matters’ and he states, that even if OS considers handicraft (slöjd) school and folk school as identical, UC wants to keep them separate; folk school as the common basic school of the society, handicraft school as a vocational school: ‘Even if we agree, that sloyd is important in the folk school, I think that the handicraftmethods must be substantially different in the common folk school and in a specialvocational school. In the former, handicrafts must be considered and handled fore mostly asa formal means of civilisation and organised accordingly, that the aim will be developmentof child’s sense for form and beauty and general dexterity, and the drill of craftsmanship of all the possible work will be avoided. In the handicraft school the aim must be dexterity invarious crafts and practising it in order to secure the sale and economic profit of theproducts. The former concept of the aim of crafts has the natural development connection to the pedagogical system of Pestalozzi and Fröbel, and it should have the undeniableimportance.’No letters from two following years can be found, between the 28th of October 1877 and the 18th of November 1879. In 1878 Uno was, however, inaugurated as the Honorary Doctor in Education in Uppsala University, Sweden. That is handled shortly in 1877 (3. June) but not at all later. The gentlemen should have met each other in Uppsala, and the proposal for honorary nomination for UC should naturally have been made by OS.(one year between)LETTERS IN 1879:The 18th of November 1879 UC writes to OS, thanks for a letter from OS and for the 3rd booklet in the publication series ‘Sloyd School and the Folk School’ which he had received, but with no further comments.On the very same day also OS is writing to UC. He handles the previous letter from UC to a Finnish teacher in Nääs, where he had expressed his idea of the difference between the Finnish school handicrafts education and the Swedish handicrafts school:‘Mr. Chief Inspector seems to think that we have different opinions concerning this matter. I do not, however, believe, that this should be the state of affairs, but I am afraid, that thereason for the misunderstanding possibly is to be found in my indistinct expression. Myidea, when I have the identification of handicraft school and folk school as a hopeful aim, is, that special handicraft schools should not exist, but that handicrafts education, whichundoubtedly is most important for the pupils in the folk school, should remain as it is.Handicrafts education can so affect at the same time as formal means of education at thesame way than the other subjects in the school. Here in Sweden we really have madeprogress in the handicraft issue recently; and it is a great pleasure to find out, how oneschool after another begins to introduce handicrafts in their programs.’The 22nd of December 1879 UC writes to OS beginning with thanks especially about theap preciation from abroad, ‘because from the home country there have been difficulties to get it’. As well UC thanks for the appreciative statement about women as teachers in Finland, because ‘they are the joy and proud of my heart’. This letter shows some di sagreement growing between the two gentlemen:‘And then some words about our different conceptions about handicraft school and folkschool. According to my idea the Folk School is a common educational institution, theHandicraft School a vocational school. The aim of the former one is to produce generaleducation and promote the development of moral characteristics of the learners; Handicraft School aims at acquiring such knowledge and skills, which are needed in a certain lifecareer. Handicraft must be taught in both schools as a subject, but in the Folk School it must be handled as the means to formal civilisation, which aims at the development of sense for form, taste and beauty, and that is why only that kind of handicrafts are appropriate, which promote that central aim. In the Handicraft School the aim of handicraft education is to give the highest possible practical professional skill in certain handicraft professions, whypractising is coloured more by drill, without which the technical fulfilment cannot easily be reached. That is why I think, that it is better to separate the schools clearly from each other, but to keep handicrafts as an important subject in both of them, even if it will be handledsubstantially differently. That is my view, but even if our views are different, we fully agree about the importance of handicraft in school. I would even claim, that handicraft education also must be introduced in secondary schools, because it has an important task, even if up to now rejected, in educati ng developmentally.’LETTER IN 1880:The 16th of April in 1880 OS writes to UC about the different opinions:‘Concerning the Handicraft School and it’s status I dare to be fully and permanentlyconvinced of us being fully at the same track and that the disagreement mentioned before is only semantic. The way of thinking I am trying to develop in my pedagogical lectures in the subject for my students, is: Handicraft School is like Folk School an institution giving basic education. It’s aim, does it work together with or separately from the Folk School, is not to develop any skills in one or more arts of handicraft in the students, but only (and this is not of minor importance) to give them general readiness of hand, in the other words skill to use hands in useful work, to teach them order and carefulness and to plant in them the will and love for work, so that it will have the pedagogical meaning forward. This power handicraft education really has, not least in it’s characteristic to become a powerful tool in the hand ofinstitution, the aim of which is to give the pupils real skilfulness in the different crafts, is a Handicraft School in the real sense of the concept. The title, the meaning of which is anyway of second order, could also be Craftsman School, Work School or Technical School. With a wish I once expressed, that Handicraft School and Folk School could be identical, I onlymeant that the independent Handicraft School should disappear, and instead it should fully be amalgamated in the Folk School, which so should also have the aims of the HandicraftSchool.‘(one year between…)LETTERS IN 1881:(The letter dated the 15th of May 1881 from UC to OS seems to be mixed with another letter sent one year later. The archives have written two dates in the (two copies of the) same letter; the presumably more appropriate original date being the 15th of May 1882.)The 29th of September in 1881 UC writes to OS. This le tter is somehow the ‘programme statement of Uno Cygnaeus’ and clarification of his ideas. It is a lengthy reply to a letter from OS the 5th of September 1881, which letter is however not included in the archives.UC tells about his childhood how his father brought him to different kinds of workshops in order to wake up the interest in handicrafts. At 12 years of age UC also ‘had the skill to use knife and lathe in woodwork and also general dexterity, which was very useful later on in life.’ When UC began his education studies it was perhaps just because of the background why he wanted to become familiar with the efforts of the Philantropic School in order to introduce handicraft to the schools: ‘I believed, however, immediately to have understood, why these efforts did not succeed.The Philantropists handled handicrafts in school very craftsman like and left it to be taught by journeymen and other craftsmen, who without any pedagogical training practised it like a craft fully neglecting the pedagogical, educational, meaning of handicrafts.After becoming familiarised more thoroughly with the writings of Pestalozzi the idea of handicraft as a formal educative tool in school became clear to me. As is generallyknown, Pestalozzi started a fight against the old scholastic grinding away and theconservatism of thoughtless memorisation and presented as an improvement among otherthings the ‘object-lessons’ and generally a teaching method educating through observational, developmental approach. It is also well-known, that Pestalozzi himself and even more hisblind imitators fell in so called observation, thinking and speaking practice in tedious, empty blabbing, which was everything else but not developmental, pedagogic. So came Fröbel and stated, that observation is not enough for a child when trying to understand with sight andtouch the artefacts and to describe them, but the children must be taught as early as possible to give form themselves for what they comprehend with their eyes and to produce something through their own work. For this Fröbel created the play tasks, which at first consisted of a ball, a cube and as a mediator a cylinder; further basketry, construction with joints,drawings, sticks, etc.’UC further praises the Fröbelian idea of play in developing the observational skills, sense of form and ‘certain general dexterity’, how these were excellent for ‘younger children and especially important in the beginning of the school’:‘But, when these tasks mostly handle only even surfaces, and dexterity g ained through these is not anymore appropriate for the older children and youth, I think, that (strictly keeping inaim to try to develop a sense for form and beauty and general dexterity, which takes theresponsibility for greater strength exertion, certain handicrafts, suitable for older pupils,should be introduced into school, fore mostly woodwork, wood turning, forge work,basketry, etc. Naturally this does not mean, that the folk school should aim to any greaterartistry in different crafts, or that the products should compete with the industrialists… - Also another mistake of Basedowian School must be avoided, handicraft education must not be considered more as refreshment and play than as a real educational medium…- The teacher must have both theoretical and practical know-how in his / her training just like in the other subjects, covering: 1. The appropriate concept about the meaning of handicrafts as a formal educational medium and, 2. Learning those grips, which are needed for properguidance in the subject’.UC tells about the pedagogical trip offered by the Czaristic Senate in 1858 to the Middle of Europe. He emphasises that he already had the idea of the meaning of handicraft education from the middle of Europe in the 1840s. At first UC came to Sweden, visited many Folk Schools and Stockholm,Örebro, Gothenburg and Helsingborg. He tells having expressed everywhere for the educators he met, ‘with the warmth of his interest and conviction’, his own:‘…perhaps in the eyes of many, not mature, but exentric efforts for reforms of education and school institution, the need for better education for women and girls, reforming readingmethodology to analytic - synthetic method, and stated the meaning of handicrafts as aformal educational means, etc’.Among the persons he met he respectfully and gratefully mentions count Thorsten Rudensköld:‘Also my proposal for introducing handicrafts education to folk school was welcomed enthusiastic, even if the proposal was something quite new.’In Copenhagen, where UC was ‘extraordinarily welcomed’ by some bishops and educators, the handicraft proposal was not considered as much as for example in Gothenburg. The colleagues were too much afraid about the possible influence of Germany considering the other pedagogical ideas: ‘As well the handicraft issue was in Denmark, if possible, even more strange than in Sweden.’Only in Hamburg the proposal was approved and welcomed, especially by an old friend, Dr. Wilhard Lange, ‘a real Pestalozzian - Fröbelian - Diesterwegian’. Likewise the proposals were approved by the Fröbelians in Berlin, among others the old Diesterweg. The best understanding of handicraft UC got from Dr. Georgen s in Vienna, who himself tried to realise the thought ‘through work for work’ in his institution in Levana. In Switzerland the proposal got less support, because at that time Fröbel was not appreciated there, ‘even if Switzerland is the country of Pestaloz zi and the modern pedagogy’.UC tells further about trying to introduce handicrafts in seminars and folk schools when he after his return was nominated to be the founder of the Finnish Folk School System, and how the‘well educated educators opposed it, b ut how the success came with the first lecturer of handicrafts in Jyväskylä with satisfactory results’. After three years of ‘experimenting’ (1863 - 66) they participated in an educational exhibition in Stockholm successfully. Also later, in 1868, in a folk school meeting in Sweden, Örebro, the Swedes, but also the Danes and the Norwegians, were positive for the exhibition.UC complains also that no conversation about the meaning of handicrafts happened, just like a few years later in Norway, Kristiania:‘We thought therefore with security, but, however, without boasting, that we can claim, that the thought of handicrafts (or slöjd) in the folk school was for the first time expressed andPestalozzi - Fröbel. The success of the idea has been quicker in other countries, especially in Sweden, because of reasons, which are easily explainable, when one knows our (Finnish)circumstances.The core of the idea and the realisation and the reason for our front line position in this matter is that we have the idea of the folk school as a formal means of civilisation,where the pedagogically trained teacher takes care of handicraft teaching. It is not done by a craftsman without any pedagogical training, not during extra hours, not only as recreation,because all this will destroy the meaning of handicrafts and gets lost when searching thepedagogic influence to the Youth.’In the following UC handles the expression 'formal mean s of education’: ‘…there has been talk that it should only be a slogan brought from Germany without a meaning.’ This UC cannot understand or acknowledge. All teaching in school can be given in different meanings, in:‘…industrial, aesthetic and military purpose forgetting and neglecting the pedagogic aim,and just here is the great and harmful mistake. In school the pedagogic, educational aimmust be the main target, so, and only so the folk school becomes a general educationinstitution to form life in general, not only towards special aims, which is the task of thevocational school. In general school there should not happen any education by chance(educatio ad hoc). The general, high aim of the folk school is education of mass, the deeprows, to real hu manity in a national sense.’UC regrets that he cannot give satisfactory answers to some questions made by OS in the last letter: ‘…because handicraft education is realised slowly in Finland, classrooms, tools and models are lacking - sometimes also visions and enthusiasm. After this we wish a better success,because now only a graduated teacher can be nominated.’Then there is some personal information: ‘…my strength is decreasing but work is increasing like avalanche…’ - UC was already 71 years of age.The 7th of November 1881 UC writes again to OS after getting no answer to his earlier letter, tells about the tighter political (Russian) pressure in Finland and uncertainty about the future. UC relies, however, on the fact that ‘in Finland nihilism, socialism or alike would never function’. He sends then a letter to Anna Roos, a Finnish teacher hired in Nääs, but as well she is not answering, maybe because of solidarity for her employer.LETTERS IN 1882:(8 letters from UC; 4 letters from OS, one of them missing, another one concerns only the travelling funds for a teacher coming to Nääs)The 25th of January in 1882 OS writes to UC about functions in Nääs. OS welcomes Finnish participants in the courses in Nääs and offers continuity for the Finnish female teachers.The 15th of May in 1882 UC writes to OS thanking for the letter from the 6th of May (which is not preserved) and refers to another letter from the 23rd of January (which cannot be found either), expects the publication of Salomon’s bookl et N:o 4 and is grateful for free vacancies for Finns in the handicraft courses in Nääs.UC underlines the status of handicrafts (sloyd) as an equal subject in the programme of every folk school, and not craftsman like but as a formally educating means, which aims at theand emphasises the folk school as a general education institution, the task of the handicraft school as vocational. The folk school, ‘the basic school of the society’, ‘basic school’ must be taken the greatest possible care of by the government and the nation organising it to be free of charge as a common school ‘for the noble and the common, for the poor and the rich,’The girls’ schools must b e strengthened with well educated teachers, so the home schools might function more properly in the future. - UC handles also the teacher exchange programme from Finland to Nääs, the high standard of handicraft education in Sortavala seminar and the pupil s’ artefacts, which will be sent to educational exhibition in Moscow.The 24th of June in 1882 OS writes to UC: OS ascertains, that:‘…the Finnish teachers returning for the courses at Nääs can proof, that the handicraftseminar (Nääs) aims to consider handicrafts basically in the same way than in the Finnishseminars and folk schools, in other words, just as Mr. Chief Inspector wants’.OS also refers to his new publication which is coming in the series, ‘…which confirms this’, and OS has:‘…dedica ted it to the man, whose discussion with good will only clarified me theunderstanding of the meaning of handicrafts as an educational means, which I have a longtime, but not clearly, pointed at, and which naturally still is more important to me, that -when Mr. Chief Inspector in his Goodness has agreed with the thoughts in my humble book - I will know, how Mr. Chief Inspector will consider those principles, which I have heardabout, to be understood properly and used accordingly in that system of handicraft in thefolk school, which I have tried to create. Because - I hope I am allowed to acknowledge this - one of my warmest wishes is, that that small publication, in which I have tried to handlethat important thing with words and deeds, to wake up interest, but I would be given thehonourable credit: ‘He belonged to the School of Uno Cygnaeus.’That the thought of handicrafts as a practical subject in the folk school is gaining more area also in other countries, is confirmed for example by participation of teachers incourses at Nääs from Norway, Denmark, Germany and France. A delegation sent by theGovernment of France to study the measures for handicraft education in different countries, has just left Nääs after an 8 days visit. Handicraft education is according to the newesteducation laws in France compulsory in every seminar and folk school.’The 8th of July in 1882 UC writes to OS as a reply to a letter from the 24th of July, 'which I would have a lot to say…’; thanks embarrassed for the unexpected appreciation... Added to that there is some information about the teachers coming to work or to participate the courses at Nääs. The same routine information is the main content in the next short letters:the 23rd of July 1882 (UC to OS);the 28th of July in 1882 (UC to OS); and the7th of August in 1882 (OS to UC).In August 1882 UC to OS: UC emphasises:‘…the satisfaction, that he has had the possibility at least a little affect for realising the great thought, education of the nation, the becoming generation, for productive work throughwork. - I do not undervalue mental work, or working with brain, work of the educated and the official, but also the so called rough work of hand must have a spiritual (mental?) motive。
Tau衰变和谱函数-IHEP
2020/5/18
BESIII物理分析讲习班
28
Tau 衰变中矢量与轴矢量流
由于 Tau 衰变是弱相互作用,宇称不守恒,因此 过程既有矢量流 (V) 也有轴矢量流 (A)。
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(n (n
)A )V
当n为奇数 当n为偶数
(KKn ) 既可以为V也可以为A
➢ 史坦福大学物理学家Martin Perl 领导的实验小组 在 1975 发现了Tau ,“Evidence for Anomalous Lepton Production in e+-e- Annihilation”,PRL, V35,1489,1975,衰变规律符合蔡先生的预言。并 于1995年获诺贝尔物理学奖。
p
0.883 GeV / c
pK 0.820 GeV / c
pK* 0.664 GeV / c
由于 Tau 的速度几乎为零,没有洛伦兹提速,因此,研究 Tau 纯轻子衰变的米歇尔能谱还具有灵敏度高的优势。
2020/5/18
BESIII物理分析讲习班
12
实验上已获取的 Tau 对事例
实验
Tau 对事例
✓ 从Tau 衰变中提取强作用耦合常数与奇异夸克质量:E. Braaten et al,”QCD Analysis of the Tau Hadronic Width”,NP,B373:581,1992
✓ A. Pich, “Tau Physics:Theory Overview”,arXiv: 0806.2793,2008
g / ge B 1.0004 0.0023
BW BW e 1.036 0.014
多细胞动物发育海绵动物门
2、形态学方面 由简单到复杂,低等到高等
从现有动物来看,有单细胞动物、多细胞动物,并形成 了由简单到复杂、由低等到高等的序列。
在原生动物鞭毛纲中有些群体鞭毛虫,如团藻,其形态 与多细胞动物很相似,可推测这类动物是从单细胞动物 过渡到多细胞动物的中间类型,即由单细胞动物发展成 群体以后又进一步发展成多细胞动物
真体腔(coelom):中 胚层之间形成的腔
1.6 胚层的分化
胚胎的继续发育形成了组织、器官结构及功能上的分化。
1.7 多细胞动物起源于单细胞动物的证据
1、古生物学方面 越古老的底层,化石种类越简单
古代动、植物的遗体或遗迹,经过千百万年地壳的变迁或造山 运动等,被埋在地层中形成了化石。
已经发现在最古老的地层中化石种类世是最简单的。 在太古代的地层中有大量有孔虫壳化石,而在晚近的地层中动 物的化石种类也较复杂,并且能看出生物由低等向高等发展的 顺序。说明最初出现单细胞动物,后来才发展出多细胞动物
2.3 分类地位
谜一般的多肉海洋无脊椎寄生动物------
有两种观点:
是原始的或退化的扁形动物?
是最原始的多细胞动物进化而来?
第三部分 多孔动物门(Porifera) (海绵动物门 Spongia) (侧生动物)
2.1 生物结构
2.1.1 体制多数不对称或辐射对称
主要生活在海水 中;固着生活; 海绵在地球上已 生存了至少5.6亿 年;---
spicules, or both B1. With triaxonid (3 axes), hexactinellid (6 rayed)
spicules ......…......... Class Hexactinellida (六放海綿
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0
EMINACT EMAXACT PTACT
Minimum sum of all photon energies required to calculate anomalous weights
Maximum sum of all photon energies allowed to calculate anomalous weights
To be submitted to Computer Physics Communications
CERN-TH/99-121 May 1999
† Work supported in part by the US National Science Foundation and Polish Government grants KBN 2P03B08414, KBN 2P03B14715, Maria Sklodowska-Curie Joint Fund II PAA/DOE-97-316, and Polish-French Collaboration within IN2P3.
Minimum sum of all photon momenta transverse to the beam direction required to calculate anomalous weights
17 GeV 1000 GeV
2 GeV
Table 1: Input parameters to control the calculation of weights for anomalous electromagnetic moments.
In order to provide the user with enough information to retrieve w for a given event for any F2(0) or F3(0), we take advantage of the fact that, for each event, one may write w as a quadratic function of the anomalous couplings:
2 Calculation of anomalous couplings
To evaluate the effects of anomalous electromagnetic couplings on radiative τ pair production, a tree–level calculation of the squared matrix element for the process e+e− → τ +τ −γ
1
3 Flags to control anomalous couplings in KORALZ
In KORALZ version 4.04, the calculation in our library is activated by setting the card IFKALIN=2. This is transmitted from the main program via the KORALZ input parameter NPAR(15). Additional input parameters are set in the routine kzphynew(XPAR,NPAR), although there are currently no connections to the KORALZ matrix input parameters XPAR and NPAR. Table 1 summarizes the functions of these input parameters.
has been carried out [4], including contributions from the anomalous magnetic dipole moment at q2 = 0, F2(0), and the electric dipole moment F3(0). This calculation is included
1 Introduction
Radiative τ pair production is of great interest, as it is sensitive to anomalous electromagnetic couplings of the τ . With the sensitivity afforded by the LEP experiments, this provides an opportunity to search for new physics phenomena [1–4]. Any meaningful interpretation of the experimental data requires a Monte Carlo simulation in which Standard Model predictions may be augmented by the contributions from possible anomalous couplings.
Parameter Description
Default
IFL1
Compute weights for F2(0) if IFL1 = 1
1
IFL2 ISFL
Compute weights for F3(0) if IFL2 = 1
0
For ISFL = −1, compute only terms with anomalous contribu-
Since the LEP collaborations are entering their final years of operation it is a good time to document the programs that were actually used in data analyses. In this paper we describe a library that has been used to calculate anomalous contributions to τ τ γ couplings [2]. The library is based on the work described in [4] and can be used with any e+e− → τ +τ −(nγ) Monte Carlo program and, after minor adaptation, with pp → Z/γ+...; Z/γ → τ +τ −(nγ) or ep → Z/γ + ...; Z/γ → τ +τ −(nγ) programs as well.
In the present paper, we will discuss the interface of our library to KORALZ version 4.04, which is described in detail in [5, 6]. The fortran code of the library is archived together with KORALZ [6], in the same tree of directories. Let us note that in the future, KORALZ will be replaced by a new program, KK2f [7], which is based on a more powerful exponentiation at the spin amplitude level; implementation of our library will be straightforward for that program as well.
in our library. When activated, it uses the 4-momenta of the leptons and the photon generated by the host program to compute a weight, w, for each event according to
tions. For ISFL = 0, include all terms (anomalous, Standard
0
Model, all interference). For ISFL = 1, use the approximation
Байду номын сангаас
of ref. [8]
IRECSOFT Generate only events with photon(s) if IRECSOFT = 1
w
=
|Mano|2 |MSM|2
.
(1)
Mano is the matrix element for F2(0) = 0 and/or F3(0) = 0, and MSM is the matrix element for F2(0) = F3(0) = 0.
As this calculation is performed at O(α), the case of multiple bremsstrahlung requires special treatment. In this case, a reduction procedure is first applied in which all photons, except the one with the greatest momentum transverse to a lepton, or pT , are incorporated into the 4-momenta of effective initial– or final–state leptons. The 4-momenta of the photon with greatest pT and the effective leptons are then used to compute the weight. Cross–checks of the calculation against an independent and slightly simplified analytical calculation [8] as well as checks of the validity of the reduction procedure are described in [9]. The results of the calculation have been used in the measurement of anomalous electromagnetic moments of the τ described in [2].