六味地黄丸对SD大鼠骨髓间充质干细胞增殖的影响

六味地黄丸对SD大鼠骨髓间充质干细胞增殖的影响
谭峰;樊巧玲;王明艳;李沐涵;尹鑫
【期刊名称】《中国医药导刊》
【年(卷),期】2011(13)7
【摘要】Objective:Observed the influences of Liu Wen Di Huang Wan water had extracted on the proliferation of bone mesenchymal stem cells (BMSCs) of SD rats in vitro.Methods:The bone mesenchymal stem cells (BMSCs) of SD rats were isolated with adherent separation method and primarily cultured in vitro,morphologically observed under inverted microscope,and identified with immunohistochemical method.The proliferation of BMSCs effected by different concentration of Liu Wei Di Huang Wan water extract were detected with MTT assay.Results:The BMSCs presented a long spindle shape and a colony growth
pattern.CD44,CD29 on BMSCs were positive and CD34 was negative detected by immunohistochemical method.MTT assay showed absorbance of BMSCs treated with Liu Wei Di Huang Wan for 24 hours at the dose of 4,2,1,0.5,0.25 mg/ml,were significantly higher than the control group (P＜0.05),especially the 1 mg/ml group(P＜0.01).Conclusion:The BMSCs isolated with adherent separation method in this experiment presented the characteristics of BMSCs both in phenotypic expression and cell morphology.Within certain range of concentrations,Liu Wei Di Huang Wan water extract could improve the proliferation activity of BMSCs in vitro.%目
的:观察不同浓度六味地黄丸水提液对体外培养的SD大鼠骨髓间充质干细胞(BMSCs)增殖的影响.方法:应用全骨髓贴壁法体外分离培养大鼠BMSCs,并传代扩增,倒置显微镜进行细胞形态学观察,免疫组化检测细胞表面抗原鉴定其表面标志.将BMSCs分为对照组及不同浓度六味地黄丸干预组,采用四甲基偶氮唑盐比色法(MTT)观察其增殖情况.结果:采用全骨髓贴壁法获取的BMSCs形态呈长梭形,呈集落样生长;免疫细胞化学鉴定,CD44、CD29阳性,CD34阴性.MTT检测结果显示,与空白对照组相比,相当于生药含量4～0.25mg/ml浓度范围内,六味地黄丸水提液干预24h后,干预组吸光度均增高(P<0.05),其中1mg/ml浓度干预组吸光度升高最明显(P<0.01).结论:所培养的大鼠骨髓细胞在表型表达和细胞形态上具备MSC特征.在一定浓度范围内,六味地黄丸水提液可以提高BMSCs的体外增殖能力.
【总页数】2页(P1226-1227)
【作者】谭峰;樊巧玲;王明艳;李沐涵;尹鑫
【作者单位】南京中医药大学,南京,210046;南京中医药大学,南京,210046;南京中医药大学,南京,210046;南京中医药大学,南京,210046;南京中医药大学,南
京,210046
【正文语种】中文
【中图分类】R285.5
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