锌影响坐骨神经损伤模型小鼠脊髓P物质表达的实验研究

锌影响坐骨神经损伤模型小鼠脊髓P物质表达的实验研究
张莉;刘明;王月静
【期刊名称】《解放军医学院学报》
【年(卷),期】2013(000)008
【摘要】Objective To study the effect of zinc on expression of substance P in mice with sciatic nerve injury (SNI). Methods Forty-eight CD1 mice were divided into control group, neuropathic pain (NPP) group, low NPP (L-NPP) group, and high NPP (H-NPP) group. A NPP model of mice was induced by surgery for SNI. Mice in control group underwent sham operation (isolating sciatic nerve) while those in N-NPP group, L-NPP group and H-NPP group underwent surgery for SNI after they were fed with forage containing zinc at the dose of 30 mg/(kg · d) and 0.85 mg/(kg · d) and with zinc sulfate water solution at the concentration of 227 mg/(L · d) respectively for 2 weeks. Effect of zinc on expression of substance P in
mice with SNI was detected by atomic absorption spectrum, immunohistochemistry, immune blotting and image analysis respectively
on day 7 after operation. Results The serum zinc level in spinal cord was significantly higher and the substance P expression level was significantly lower in H-NPP group than in L-NPP group (P<0.01).Conclusion Zinc can inhibit the expression of substance P in mice with SNI.% 目的研究锌对选择性坐骨神经分支损伤(spared nerve injury，SNI)小鼠脊髓P物质(substance P，SP)表达的影响。

方法48只CD1小鼠分为4组，采用SNI手术制备神经病理性疼
痛(neuropathic pain，NPP)小鼠模型：对照组(Con)给予假手术(单纯分离坐骨神经)，适锌喂养神经分支损伤(N-NPP)组锌饲料30 mg/(kg·d)喂养2周后给予SNI 手术，低锌喂养神经分支损伤(L-NPP)组锌饲料0.85 mg/(kg·d)喂养2周后给予SNI手术，高锌喂养神经分支损伤(H-NPP)组硫酸锌水溶液227 mg/(L·d)喂养2
周后给予SNI手术。

应用原子吸收光谱、免疫组织化学、免疫印迹和图像分析技术检测术后7d锌对脊髓SP表达的影响。

结果高锌喂养能显著增加血清和脊髓中锌的含量，脊髓SP表达下调(P＜0.01)；而低锌喂养加重血清和脊髓的缺锌状况，脊髓SP表达上调(P＜0.01)。

结论锌能抑制SNI后小鼠脊髓SP的表达。

【总页数】4页(P876-879)
【作者】张莉;刘明;王月静
【作者单位】辽宁医学院组织学与胚胎学教研室，辽宁锦州 121001;辽宁医学院组织学与胚胎学教研室，辽宁锦州 121001;辽宁医学院组织学与胚胎学教研室，辽宁锦州 121001
【正文语种】中文
【中图分类】R741.02
【相关文献】
1.痛必定注射剂对坐骨神经结扎慢性损伤模型大鼠脊髓背根神经节P物质含量的影响 [J], 张昊旻n;王中男
2.锌影响坐骨神经损伤模型小鼠脊髓P物质表达的实验研究 [J], 张莉;刘明;王月静;
3.氟比洛芬酯对坐骨神经慢性缩窄性损伤模型大鼠肌肉中P物质及神经速激肽1
受体表达的影响 [J], 毛豪丽;刘春芳;徐辉;姜虹;陈志峰
4.微囊化兔坐骨神经组织细胞移植对大鼠脊髓半横断脊神经节P物质表达的影响[J], 虞琴;王向义;陈洪;刘德明
5.鞘内注射地塞米松对坐骨神经慢性压迫损伤大鼠脊髓背角降钙素基因相关肽和P 物质表达的影响 [J], 胡进宇;王英伟
因版权原因，仅展示原文概要，查看原文内容请购买。