4 种食用菌提取物自由基清除作用及降血糖作用的研究

4种食用菌提取物自由基清除作用及

降血糖作用的研究

王峰1，陶明煊1，程光宇2,*，陈宇1，李翊俊1

(1.南京师范大学金陵女子学院，江苏南京 210097；2.南京师范大学生命科学学院，江苏南京 210097)

摘 要：目的：研究金顶侧耳提取物(PCTE)、大球盖菇提取物(SRE)、姬菇提取物(PCNE)和柳松菇提取物(AAE)对自由基清除作用及对糖尿病小鼠降血糖作用。方法：采用分光光度法分析4种食用菌提取物(EFE)中抗氧化成分和自由基清除能力。小鼠腹腔注射四氧嘧啶建立动物糖尿病模型，以EFE(400mg/kg bw)分别灌胃糖尿病小鼠28d，同时以降糖灵(100mg/kg bw)为药物治疗组，以正常小鼠为对照组，测定0、6、14、21、28d时空腹血糖值和28d时糖耐量。动物处死后，取血液测定小鼠糖化血红蛋白(GHB)含量及血脂水平。结果：4种EFE总黄酮和总酚分别在2.37～6.89mg/g和2.57～4.96mg/g间；多糖含量在20.30～44.02g/100g间。清除超氧阴离子自由基、羟自由基、DPPH自由基EC50浓度分别为PCTE：0.32、2.36、14.51mg/ml；SRE：0.24、3.71、15.32mg/ml；PCNE：

0.36、2.60、15.36mg/ml；AAE：0.13、3.95、11.20mg/ml。与模型组比，灌胃EFE 28d后能明显改善糖尿病

“三多一少”病症，能显著降低空腹血糖值(P＜0.01)和血糖曲线下面积(AUC)(P＜0.01)，其中给予PCTE、PCNE、AAE后血糖下降百分率都大于15%，并使GHB含量恢复到正常水平(P＞0.05)。4种EFE还能使糖尿病小鼠总胆固醇(TC)和高密度脂蛋白(HDL-C)含量恢复到正常水平。结论：PCTE、SRE、PCNE和AAE能有效地清除自由基，对糖尿病小鼠有较好的降血糖、改善糖耐量作用，并有一定的降血脂作用。

关键词：食用菌提取物；自由基；抗氧化作用；糖尿病；降血糖作用

Free Radical Scavenging Activities and Hypoglycemic Effects of Aqueous Extracts of Four Species of Edible Fungi

WANG Feng1，TAO Ming-xuan1，CHENG Guang-yu2,*，CHEN Yu1，LI Yi-jun1

(1. Ginling College, Nanjing Normal University, Nanjing 210097，China；

2. College of Life Sciences, Nanjing Normal University, Nanjing 210097, China)

Abstract ：Kunming male mice (about 6 weeks old, (20 ± 2) g) were used as experimental animals for measuring free radical scavenging activities and hypoglycemic effects of aqueous extracts of 4 species of edible fungi, including Pleurotus citrinopileatus, Stropharia rugoso-annulata, Pleurotus cornucopiae and Agrocybe aegerita. Meanwhile, active components in the aqueous extracts, including total flavonoids, total phenols and polysaccharides were quantified by spectrophotometric methods. Alloxan-induced diabetic mouse models were constructed by intraperitoneal injection and intragastrically administered with a dose of 400 mg/kg for continuously 28 d, using phenformin-treated mice (100 mg/kg) as positive group and normal mice as control group.

Fasting blood glucose level was determined on the 0, 6th, 14th, 21st and 28th d, and glucose tolerance level was determined on the 28th d. After scarification, mice were subjected to blood sampling for assaying glycated hemoglobin and blood lipid levels.

These aqueous extracts exhibited a total flavonoids content ranging 2.37－6.89 mg/g, a total phenols content ranging 2.5－4.96 mg/g and a polysaccharides content ranging 20.30－44.02 g/100 g. The EC50 values of Pleurotus citrinopileatus, Stropharia rugoso-annulata, Pleurotus cornucopiae and Agrocybe aegerita extracts (PCTE, SRE, PCNE, AAE) were 0.32, 0.24, 0.36, 0.13 mg/ml for scavenging superoxide radicals, 2.36, 3.71, 2.60, 3.95 mg/ml for scavenging hydroxyl radicals, and 14.51, 15.32, 15.36, 11.20 mg/ml for scavenging DPPH radicals, respectively. The 28-day continuous administration of each of these aqueous extracts

收稿日期：2008-11-27

基金项目：江苏省高校自然科学基础研究项目(07KJD550116)

作者简介：王峰(1982－)，女，硕士研究生，研究方向为营养与保健功能因子。E-mail：mifeng_2008@

*通讯作者：程光宇(1954－)，男，高级实验师，研究方向为生物活性物质与保健功能因子。E-mail：mxwcgy@