A Graph-Theoretical Approach for Pattern Matching in Post-Tonal Music Analysis

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清华大学遗传算法PPT

清华大学遗传算法PPT
2.1 Basic Concept of dc-MST 2.2 Genetic Algorithms Approach 2.3 GA procedure for dc-MST 2.4 Numerical Experiments
3. Degree-based Permutation GA for dc-MST
4.1 Basic Concept of lc-MST 4.2 Genetic Algorithms Approach 4.3 GA procedure for lc-MST 4.4 Numerical Experiments
Soft Computing Lab.
WASEDA UNIVERSITY , IPS
Stochastic MST
Ishii, H., H. Shiode, & T. Nishida: Stochastic spanning tree problem, Discrete Applied Mathematics, vol.3, pp.263-273,1981.
Quadratic MST
Leaf-constrained MST
Fernandes, L. M. & L. Gouveia: Minimal spanning trees with a constraint on the number of leaves, European J. of Operational Research, vol.104, pp.250-261, 1998. Soft Computing Lab. WASEDA UNIVERSITY , IPS 7
3.1 Concept on Degree-based Permutation GA 3.2 Genetic Algorithms Approach 3.3 Degree-based Permutation GA for dc-MST 3.4 Numerical Experiments

半导体光刻技术

半导体光刻技术

半导体光刻技术OntheleftofthisslidetheamplitudeoftheFouriercoefficientsisshownfortheordersfromthegratingandtheprojectedorders(onthewafer).Thisleadstoacomparisonbetweentheperfectimageandthesumoforders.Ontherightsidethesameisdoneforagratingwithasmallerpitch.NotethattheevenordersarenotpresentintheFouriercoefficients!!MOS产品事业部NIKON光刻机光刻要求温度23+/-1度,湿度45+/-度13131312121212121212114)Developconditions显影液浓度影响显影液温度影响:23度最合适显影时间的影响1.502.383.0TMAHConcentrationPhotospeedResolutionBetterHigherDeveloptimevsprofileDeveloptimevsprofile?time(sec)PROFILE5060409070OptimumtimeThisisthestandardtooltodecidedevelopdippingtime.UnderDevelop:ScumandtailOverDevelop:Topprofilecollap seDEVELOPRATETEMPERTUREDeveloptemperatureisoneofimportantitemtocontroldeveloprate.Ithasoptimumtemp.ButTomaintainconstanttemperatureisnoteasy.ItdependonspraytypeanduallyEngineerneedtofindoutoptimumtemp.Butusuallywecanuseroomtemperaturebecausethistemp.isthemoststabletemperature.3?DeveloptempvsDevelopspeed(rate)3.光刻⼯艺交流SwingcurveThereareinterferencebetweeninputandreflectivelightintheresistfilm.Theamplitudeofstandingwaveisdecidedbywavelength,substrateandARCfilmTheworstcaseisDUVresist(becauseofhighReflectivity)UsingTARCisbest:TARCreducesstandingwaveeffect.TprshouldbedecidedbyminimumCDvariationtarget(HillorvalleyofstandingwavecurveFEMDefocus的影响Defocus产⽣的原因ParticleEdgeEffectAutoFocusAutoLeveling边缘效应FunctionDecideoptimumz-stageposition(Focus)beforeshotexposing.INCIDENTBEAMDetector(CCD)?ZZ-MOTION?XQQ''PROJECTIONLENSMIRRORMIRRORLENSLENS?WAFERLightsource:HalogenlampDetector:7x7(49points)sensorDatafeedback:z-stage?X=2?Zsin?:When?is90degree,AFsensitivityisbestThefeatureofAFsensorisdifferentfromeachvendor.Itdependonwafershotposition:Waferedge==>Beamsizeistoolarge:ShiftfocusThisisnotthesameasAutoLevelingsystem.ThisfeatureshowCanonsystem.Autofocussystem5AutoLevelingMisalignOffsetInShotMagnificationShotRotationByShotsScalingWaferRotationOrthogonilityMisalign光刻胶、显影液等原料⼯艺评估和验收光刻胶:spincurve,swingcurve,masklinearity,resolution,processwindow,etchandimplantresistance显影液:defectmonitor,resistsprofile,CD,developrate.负胶:曝光后变为交联聚合物,不溶于显影液;正胶:曝光后光敏剂分解,打断交联化学键负胶:曝光后变为交联聚合物,不溶于显影液;正胶:曝光后光敏剂分解,打断交联化学键Inthisslideabundleoflight,drawnaswavefronts,passesthroughadoubleslit.AccordingtoHuygens’principle,discussedearlier,thewavefrontswhichpassthegratingactlikenewformedcircularwavefronts.Thesecircularwavefrontsinterferetogivemaximumandminimumintensity.TheMaximumintensitiesarecalledorders.Whenthereticleimageisconstant,theangleofthediffractedorderswillbebiggerifthewavelengthislonger,i.e.248nmthanifthewavelengthisshorter,i.e.193nm.Inthisexample,theverticalparallellinesdefinetheabilityoftheprocesstoresolveanimage.Thetopdrawingshowstheordersrecombiningatawideangleresultingfromilluminationatalongerwavelength,forexample248-nm.Thebottomdrawingshowstheordersfromthesamefeaturerecombiningatanarrowerangleresultingfromilluminationatashorterwavelength,forexample193-nm.Inbothcases,theprocessresolvesthesameminimumimagesize.Howeverinthebottomexamplethedistancefromtheexactpointofrecombinationoforders(thefocalplane)isgreaterthaninthetopexamplesimplybecausetheangleofdiffractionisnotaslarge.Therefore,thebottomexampleallowsagreatertoleranceinfocusbeforetheabilitytoresolvethisminimumimagesizeislost.DiffractionordersTheleftsideofthisillustrationshows(intwodimensions)howthediffractionangleofordersbecomesincreasinglylarger(fromnormal)astheorderincreases.Therightsideoftheillustrationaddsdepthtotheinformationontheleft,asiflookingintotheslide.So,whatareweseeing?Ontheright,thefullimplicationofthewavefrontsreachingthewaferareshown(goingthroughthewafer,asiftransparent).Thetheoreticalmaskimageisshownatthewaferplponent(0thorder)ofthedenselinesisshowntobeilluminatingthefullfield.Thecenterviewshowsonlythepositivefirstorderinformation,anddefineshowthewavefrontapproachesthewaferatitsangleofdiffraction.Thebottomviewshowsonlythepositivethirdorderinformation,againdefininghowthewavefrontalsoapproachesthewaferatitsangleofdiffraction,beingagreaterangle(3X)thanthatofthefirstorder,andarrivingatthewaferwithtriplethespatialfrequencyofthemaskimage.Thisillustrationexplainshowdiffractedinformationisrecombinedtoformanimage.Thereticlepatternisagrating:thatis,repeatingclearandopaquefeatures.Forsuchasituation(50%dutycycle),the0thorderisadccomponentwhichisequalinamplitudetotheaverageoflightanddarksegmentsofthegrating.Asthirdorderinformationisresolvedbythelens(largerfeatures),the“sum”curveatthebottomshowshowtheresultingimageatthefocalplanebeginsmoretoresembletheoriginalreticlefeature(squarewave).AlignmentProcessAlignmentMarkLSAvs.FIA对位⽅式光源优点缺点LSA激光灵敏度⾼,识别能⼒强,适合绝⼤多数层次不适合铝层及对位标记不对称的情况FIA卤素灯适合铝层及⾮对称对位标记对位速度慢,对台阶要求⾼WaferSearchAlignmentSearchY-T对位;确认圆⽚旋转;确认Y位置;确认X位置;进⾏EGA对位。

欧洲肿瘤协会主席David Kerr,新的医学范式 - 个体化医学,通过检测生物标志物进行疗效预测P

欧洲肿瘤协会主席David Kerr,新的医学范式 - 个体化医学,通过检测生物标志物进行疗效预测P

Laboratory of Cancer Biology, Department of Clinical Pharmacology (N . B . La Thangue ), Nuffield Department of Clinical and Laboratory Sciences (D . J . Kerr ), University of Oxford, Old Road Campus Research Building, Old Road Campus, Oxford OX3 7DQ, UK.Correspondence to: N. B. La Thangue thangue@Predictive biomarkers: a paradigm shift towards personalized cancer medicineNicholas B. La Thangue and David J. KerrAbstract | Advances in our understanding of the intricate molecular mechanisms for transformation of a normal cell to a cancer cell, and the aberrant control of complementary pathways, have presented a much more complex set of challenges for the diagnostic and therapeutic disciplines than originally appreciated. The oncology field has entered an era of personalized medicine where treatment selection for each cancer patient is becoming individualized or customized. This advance reflects the molecular and genetic composition of the tumors and progress in biomarker technology, which allow us to align the most appropriate treatment according to the patient’s disease. There is a worldwide acceptance that advances in our ability to identify predictive biomarkers and provide them as companion diagnostics for stratifying and subgrouping patients represents the next leap forward in improving the quality of clinical care in oncology. As such, we areprogressing from a population-based empirical ‘one drug fits all’ treatment model, to a focused personalized approach where rational companion diagnostic tests support the drug’s clinical utility by identifying the most responsive patient subgroup.La Thangue, N. B. & Kerr, D. J. Nat. Rev. Clin. Oncol. 8, 587–596 (2011); published online 23 August 2011; doi:10.1038/nrclinonc.2011.121IntroductionThere is little doubt that cancer is one of the most impor­tant healthcare issues facing our society. Cancer is the second most common cause of death in the Western World, where the lifetime risk of developing cancer is approximately 40%.1 The reasons for this high lifetime risk are multifactorial and include environmental and lifestyle changes. The average lifespan of Western popula­tions is increasing and, therefore, so is the risk of develop­ing cancer. The overall annual costs of cancer measured in pure economic terms, namely direct medical expenses and lost productivity, is increasing at an exponential rate, and in 2008 was estimated to be $228 billion in the USA alone, and £18.3 billion in the UK.2,3The term ‘cancer’ defines over one hundred differ­ent diseases that can arise from virtually any tissue or organ in the body and, while sharing common prop­erties of local invasion and distant spread, may have different causative factors, molecular composition, natural history of disease, methods for diagnosis and methods by which they are treated. Consequently, modern develop m ental therapeutics requires that a new treatment must not only address a disease defined by the histology and anatomical site from which it arose but also the specific molecular, genetic or immuno­logic subtype. Importantly, the advances in biomarker techno l ogies and how best to deploy them in the clini­cal setting means that treating cancer has progressedfrom a ‘one drug fits all’ approach to a more ‘personal­ized’ strategy where treatment regimens are driven by biomarker expression profiles.4–6There are different types of cancer biomarkers; prog­nostic, pharmacodynamic and predictive.7 A prognostic biomarker anticipates the likely outcome of the illness and may, if appropriate, dictate whether further therapy is required. For example, the benefits of adjuvant chemo­therapy for stage II colon cancer have been long debated, and various histopathological factors such as T stage, vascular invasion and tumor grade have been used to describe a subgroup considered to be of higher than average risk of recurrence and, therefore, used to select patients who might benefit from an improved absolute survival gain from adjuvant chemotherapy.8,9 More recent versions of prognostic biomarkers include the Onco type DX ® test (Genomic Health, Redwood City, CA), which is a transcript­based assay that forecasts the probability of breast cancer recurring after surgical intervention.8 Pharmacodynamic biomarkers measure the effect of a drug on the disease;10 for example, the level of prolif­eration and apoptosis in the tumor upon delivery of a drug, or the degree of change on a substrate regulated by an enzymatic drug target (such as phosphorylation after inhibition of a protein kinase).11 By contrast, predic­tive biomarkers assess the likelihood that the tumor will respond to the drug, and thereby allow a level of person­alization to be introduced into the treatment regimen. There are a small number of predictive biomarkers that have found clinical utility,12 and others are gaining clini­cal acceptance as objective measurements that inform on the clinical response to the drug (Table 1).Competing interestsN. B. La Thangue and D. J. Kerr have declared an association with the following company: Celleron Therapeutics. See the article online for full details of the relationships.REVIEWSThe need for predictive biomarkersSupport for the development of predictive biomarkers for drug response is wide and varied, and encompasses regulatory, commercial and clinical standpoints. These considerations are relevant to many recently developed cancer drugs that target discrete molecular aberrations, which are usually effective on only a subset of the patient population, typically in the region of 10–20%.13 This means that for the annual spend on cancer drugs (esti­mated to be $60 billion in 2010), without patient selec­tion about $45 billion will be spent on medicine that provides limited benefit. As such, there is a clear and compelling argument to develop companion diag n ostics that measure biomarkers which, in turn, identify the responsive subpopulation of patients. This will improve the cost effectiveness of the therapy, and go hand­in­hand with improved clinical benefit and safer drugs. Furthermore, certain chemotherapy regimens result in death rates in the range of 0.5–2.0%, and 30–40% of patients experience grade 3 or 4 toxic effects,14 represent­ing a large burden of morbidity, especially if a signifi c ant fraction of this population do not benefit from treat­ment. Targeted therapies can cause similar levels of toxicity. For example, bevacizumab causes serious side effects including gastrointestinal, cardiovascular and renal toxicity.15 One important element of predictive biomarker­driven cancer therapy will be the reduction in unnecessary treatment and adverse effects.The changing pharmacoeconomic environment and the escalating cost of drugs has led regulatory bodies to emphasize the importance of predictive biomarkers, and the benefits that a predictive biomarker assay pro­vides to patients and payer’s budgets. Both the European Medicines Agency (EMA) and FDA are encouraging drug developers to identify predictive biomarkers as a companion diagnostic, which is widely accepted to become a more­common guideline and general prac­tice.16 In general, there is an increasing demand for predic t ive tools to allow patients with responsive disease to be identified and treated accordingly.The expansion of our knowledge of cancer biology has created many different options for new types of bio­markers and there is now the possibility of achievingselective and specific personalized cancer treatments. However, there are consequences that result from tumor subtyping and personalizing cancer treatments. Older classes of drugs, such as nonspecific cytotoxic chemotherapeutic agents, which target general mecha­nisms shared between many different types of tumors, are usually used on a very broad spectrum of tumor types and hence are often widely employed. Usually, these agents are off patent and, therefore, generic and of significantly lower cost compared with the molecularly targeted drugs.17 The commercial incentive to develop companion diagnostics for generic drugs is not com­pelling, which contrasts with the argument for thera­peutic benefit. Conversely, highly targeted therapies that are aimed at discrete molecular aberrations will have their application restricted to a subset of patients whose tumors display the required biomarker. These newer targeted agents are frequently of high cost, and subject to economic scrutiny by regulatory authorities when the manufacturer seeks marketing approval.17 In such cases, there is clear justification (reflecting com­mercial, clinical and regulatory arguments) to develop companion diagnostic support.We have highlighted some of the predictive bio­marker and companion diagnostic tests that are gaining increasing acceptance in the cancer clinic. Where appropriate we have detailed the pitfalls and the his­torical bottlenecks that were experienced in developing the current gamut of tests.Predictive biomarkers in useHER2 and breast cancerBreast cancer is the most frequently occurring cancer in women.18 The HER2 gene is amplified and overexpressed in about 25% of tumors, and patients with HER2­positive tumors have a poorer prognosis than other types of breast cancer; for example, approximately 80% of patients with invasive ductal carcinomas show HER2 amplification.19–21 Trastuzumab is a recombinant human­ized monoclonal antibody that targets the HER2 protein (Figure 1), and was developed on the basis that tumors over e xpressing this target would respond favorably.22 Trastuzumab was the first targeted therapy approved by the FDA for metastatic breast cancer in combina­tion with adjuvant treatment regimens (doxorubicin, cyclophosphamide and paclitaxel) for node­positive,HER2­overexpressing tumors.23In several large clinical studies, trastuzumab had a major impact on HER2­positive metastatic breast cancer, and in combination with chemotherapy was suggested to increase both survival and response rate compared with trastuzumab alone.21,24,25 However, about 70% of HER2­positive patients do not respond to the drug, and resistance to the treatment develops rapidly (within a year of treatment) in virtually all patients.22,26,27 Resistance mechanisms are an active area of investiga­tion.28 In addition, a number of multicenter randomized studies have reported significant benefit from the addi­tion of trastuzumab to adjuvant therapy with up to 50% reduction in the relapse of breast cancer.22The need for accurate detection of HER2 status as a pre­dictive biomarker is important as therapeutic decisions have been increasingly influenced by the level of HER2 expression. The ability to reliably identify patients who might benefit from trastuzumab is not only important for clinical reasons (a significant proportion of grade 3 or 4 cardiotoxicity is associated with treatment27) but also for economic ones (trastuzumab costs about €42,000 per treatment course17). The most commonly used compan­ion diagnostic tests are the HercepTest™ (Dako, Glostrup, Denmark) and Ventana Pathway (Ventana Medical Systems, Tucson, AZ), both representing standard­ized tests that measure HER2 protein expression levels by immunohistochemistry (IHC) in tumor biopsies.29 However, the ability of HER2 expression status to predict the benefit of trastuzumab is a subject of much debate, as it seems to be modest at best with a positive predictive value (PPV) usually in the region of 25–40%.30 IHC has numerous limitations—technical and interpretative—that impact on reproducibility and accuracy.The increasing number of tests available for measur­ing HER2 levels has complicated this area. For example, a recent study indicated that one in five HER2 tests gave the incorrect result,31 and more generally a large pro­portion of patients treated with trastuzumab are never tested for HER2 expression.30–32 Currently, the debate on how best to select patients that respond favorably to trastuzumab also favors fluorescence in situ hybridiza­tion (FISH) to measure HER2 amplification.31,32 Thus, more than a decade after trastuzumab was approved there remain many obstacles in the practice of identify­ing responder patients and delivering personalized care, a situation that epitomizes the personalized medicine paradigm. With new opportunities for tests being devel­oped,29 and trials underway with trastuzumab in patients with earlier disease stage, it is likely that more biomarker tests will be added to the armamentarium to assist in guiding the personalization of breast cancer therapy.CML and imatinibChronic myeloid leukemia (CML) is a hemato­logical malignancy characterized by high prolifera­tion of myeloid cells. Most CML cases are associated with a specific chromo s omal translocation between chromosome 9 and 22 resulting in the characteristic Philadelphia chromosome, creating a fusion protein, BCR–ABL, which acts as a constitutively active tyrosine kinase.33 A selective inhibitor of BCR–ABL, imatinib,34 is highly effective against CML, and is now an established first­line therapy.35 However, resistance to imatinib occurs in about 10–15% of patients, which frequently is caused by mutations in the gene encoding the catalytic domain that prevent inhibition by imatinib, although BCR–ABL indepen d ent mechanisms can also occur.36 It is estimated that 30–50% of patients with secondary resistance to imatinib have a catalytic domain mutation, and over 100 different mutations have been identified.36 Consequently, inhibitors that act on imatinib­resistant mutants have been developed, and include drugs such as dasatinib and nilotinib (Table 1). Dasatinib is active against most imatinib­resistant BCR–ABL­positive tumors, and inhibits proliferation of BCR–ABL progeni­tor cells from patients with imatinib­resistant disease.36 This drug was approved for treatment of CML patients with resistance to imatinib.37–39 Several other BCR–ABL tyrosine kinase inhibitors are either approved or under development, such as nilotinib, a more­potent inhibi­tor than imatinib.38–40 Both dasatinib and nilotinib have activity against other kinases, which might contribute to their activity in imatinib­resistant disease.40–42 The level of resistance to imatinib, nilotinib and dasatinib depends on the mutation identified43 as some mutations that result in amino acid substitutions, such as Tyr315Ile (which occur at the contact site between the P­loop and kinase domain), impart resistance to all three agents.41 Biomarkers for hematological malignancies have tra­ditionally been assessed through cytogenetic analysis, such as identification of the Philadelphia chromosome in CML.40,42 More sophisticated molecular approaches are now being combined with conventional analysis,44 and catalytic domain mutation screening for imatinib resistance has added another level of diagnostic complex­ity.45 Mutation in the catalytic domain of BCR–ABL that confers resistance to imatinib is used as a predictive bio­marker for identifying patients that should be treated with dasatinib or nilotinib, and several companion diagnostics are available that enable these patients to be identified to receive the most appropriate treatment.45,46REVIEWSImatinib also inhibits the receptor tyrosine kinase KIT, which led to clinical trials in patients with gastro­intestinal stromal tumors (GIST), where antitumor activity has been observed in this poorly responsive disease.47,48 Activating mutations in KIT correlate with drug response indicating that KIT mutations might be useful as a response­specific biomarker.49EGFR and other biomarkersEGFR is a member of the HER/ERB family of receptor kinases (Figure 1). It represents an important therapeutic target because it is frequently overexpressed and mutated in a number of cancers, including colorectal cancers (CRC) and non­small­cell lung cancer (NSCLC), affect­ing some 30% of carcinomas.50 Both monoclonal anti­bodies and small­molecule tyrosine kinase inhibitors that target EGFR have become accepted therapies for both of these solid cancers.50 Cetuximab was the first anti­EGFR monoclonal antibody to be approved for metastatic CRC, closely followed by panitumumab, which has demon­strated activity in metastatic chemo r efractory CRC.51,52 Both antibodies have similar efficacy and provide modest but clinically meaningful response rates of approximately 10% when used as monotherapy.50In the initial clinical trials, the protein expression of EGFR was evaluated as a possible predictive biomarker at trial entry based on the assumption that EGFR expression would correlate with sensitivity to EGFR inhibitors.50,53,54 However, a number of studies have shown that EGFR protein levels are poorly correlated with response in the clinical setting;50 objective responses occur in patients with low or no EGFR expression as well as high EGFR expression.50,54 The uncertainties surrounding the clinical value of EGFR expression led to the search for alternative biomarkers to identify patients that respond favorably to EGFR­targeted therapies.Oncogenic activation of pathways downstream of EGFR involves a well­defined cascade that engages a variety of signaling proteins, including KRAS, RAF and PI3K (Figure 1). Mutation in KRAS results in continuous activation of MAPK or PI3K signaling, independently of EGFR activation;55KRAS is the most common gene mutation in the pathway (occurring in 35–45% of CRCs). Through retrospective analysis, the status of KRAS was found to be an important predictive biomarker for poor response to cetuximab and panitumumab.14,56 Patients with tumors harboring KRAS mutations did not respond favorably or experience any survival benefit; progression­ free survival was approximately half that of patients expressing wild­type KRAS.57,58KRAS is considered a predictive drug­response­specific biomarker, and the use of KRAS mutation as a diagnostic biomarker is increas­ingly being used to select patients who are unlikely to respond to these agents. In Europe, both panitumumab and cetuximab are indicated for patients with KRAS wild­type CRC, which is supported by a companion diag­nostic (KRAS mutation detection); in the USA, a recent label update indicates that cetuximab and panitumumab treatment is no longer recommended for patients with KRAS mutations.59,60NSCLC is the leading cause of cancer mortality, with EGFR activating mutations occurring in about 10% of cases.50 As a consequence, two EGFR tyrosine kinase inhibitors were developed and subsequently approved for advanced­stage NSCLC, namely gefitinib and erlo­tinib.50,61 Erlotinib and gefitinib show modest activity in NSCLC patients, although activating mutations that occur in EGFR correlate with a higher response rate.62 Studies have shown that patients with tumors with exon 19 deletion EGFR mutations have longer survival fol­lowing treatment.63–66KRAS mutations, which occur in 15–30% lung adenocarcinomas, have also been found to be associated with reduced response to these inhibi­tors.50,67 Thus, KRAS mutation may similarly provide a response biomarker for patients that will not benefit, in contrast to EGFR activating mutations, which provide a predictive biomarker for a positive response. Oncogenic rearrangements of the ALK gene have also been described in NSCLC, which occurs in about 3–5% patients and are mutually exclusive with EGFR muta­tions.68 Crizotinib is a targeted therapy against ALK that has shown encouraging response rates in patients with a rearranged ALK gene in a predictive biomarker­driven clinical trial.69tyrosine kinase portion of EGFR and thereby prevent the receptor-mediated signaling pathway from being activated.In glioblastoma, EGFR is overexpressed in 40–90% of cases, usually due to gene amplification.70 Clinical trials have suggested that the response to erlotinib or gefitinib is independent of EGFR amplification.71 Response is associated with the co­expression of PTEN, which is fre­quently mutated in glioblastoma leading to activation of the PI3K/AKT signaling pathway71,72 (Figure 1). Whilst studies on the value of PTEN as a predictive biomarker in glioblastoma are ongoing,72 this finding does potentially provide an important means to subgroup patients into a responder population.APL and all trans retinoic acidAcute promyelocytic leukemia (APL) accounts for 10% of all acute myeloid leukemias. More than 99% of APL cases harbor a translocation between chromosome 15 and 17, which fuses the retinoic acid receptor (RAR)α gene on chromosome 17 with the PML gene on chromosome 15, resulting in a PML–RARα fusion protein.73 Detection of the PML–RARα t(15:17) translocation is regarded as a diagnostic biomarker for APL, and used to define the most appropriate treatment regimen.73,74We now have an understanding of how the PML–RARα fusion impacts on clinical response. The PML–RARα fusion protein binds to retinoic acid response elements in the promoters of target genes which, together with its hetero­dimeric partner protein RXR, recruits co­repressors, such as SMRT and N­CoR, and histone deacetylase (HDAC), with subsequent repression of retinoic acid responsivetarget genes.74 The PML–RARα fusion protein binds co­repressors more avidly than the natural RARα protein, so physiological levels of all trans retinoic acid (ATRA) cannot overcome transcriptional repression mediated by the fusion protein.75 Consequently, understanding the mechanism of action of the PML–RARα fusion protein led to disease remission in 90% of newly diagnosed patients.76 ATRA therapy results in the differentiation of APL blast cells at pharmacological concentrations (10–6 M), but not physiological concentrations (10–9 M), and thus the higher dose became the relevant treatment regimen.76 However, relapse frequently occurs within months fol­lowing treatment,76,77 which is now routinely followed by a chemotherapy combination.78 Relapsed patients respond favorably to a combination therapy with arsenic tri o xide, which has been suggested to target the PML–RARα fusion protein for degradation.78 HDAC inhibitors also enhance ATRA­induced differentiation in NB4 cells, through a mechanism thought to reflect inhibition of HDAC and dissociation of the N­CoR/SMRT complex from PML–RARα.79,80 Overall, the PML–RARα translocation correlates with response to ATRA and arsenic trioxide.78 The detection of the translocation event has provided an important historical approach and a widely used predictive biomarker for diagnosis and treatment.PARP inhibitors and BRCA deficiencyThe breast cancer susceptibility genes BRCA1 and BRCA2 encode proteins that are components of the homolo­gous recombination (HR) DNA­repair pathway, and mutation in either gene enhances cancer susceptibility resulting in a 60% chance of developing breast cancerby the age of 90 years.81 The fact that many pathwaysin mammalian cells are redundant has been exploited inthe context of BRCA1/2 deficiency and targeted therapy.The thera p eutic strategy is based on the rationale that anaberration in one pathway, for example HR, renders cellsmore sensitive to specific inhibition of the compensatorypathway which, otherwise, would be non­essential.82 Thisphenomenon of ‘synthetic lethality’ provides a potentiallypowerful approach to cancer treatment, and is illustratedwell by the clinical strategy and anticipated utility ofinhibitors of poly­ADP(ribose) polymerase (PARP) inBRCA1/2 deficient tumors.83PARP is a DNA­damage­sensing nuclear enzymeinvolved in DNA repair.84 In most cell lines, treatmentwith PARP inhibitors has minimal effect on prolifera­tion. However, breast cancer cells defective in BRCA1 orBRCA2 are highly sensitive to PARP inhibition, becauseof the role that each protein plays in the HR pathway,and the absence of redundant pathways.85 Thus, inBRCA1 and BRCA2 defective cells, DNA damage nor­mally repaired by the HR pathway remains unrepaired,and inhibition of PARP increases the prevalence of thedamage, ultimately providing a signal for apoptosis.83,85Importantly, PARP inhibition is not critical for normalcells, most likely reflecting intact redundant pathways,so PARP inhibitors have the potential to impair tumorgrowth without damaging normal cells.85 To date, clini­cal trials have shown promising results in BRCA1 andBRCA2 defective tumors, and in a range of tumorsFigure 2 | Design of biomarker testing clinical trials. In a | a biomarker is assessed using historical data collected from previously conducted clinical trials where archived tissue from treated patients is compared for the expression of the biomarker in a retrospective analysis. In b | the biomarker status of all patients is used as a stratification factor in a prospective trial where each subgroup is subsequently randomized for treatment. The sample size is specified separately within each biomarker-based subgroup.REVIEWSPARP inhibitors enhance the cytotoxicity of a variety of agents.86,87BRCA1/2 associated cancers account for only about 5% of breast cancer cases; however, they share a number of morphologic and molecular features with basaloid, triple­negative breast cancer, a subgroup that is notori­ously difficult to treat.88,89 O’Shaughnessy et al.89 con­ducted a randomized phase II trial in patients with triple­negative breast cancer, to compare carboplatin plus gemcitabine with or without the PARP inhibitor iniparib. There was a greater degree of durable tumor stabilization in the PARP inhibitor arm (54% versus 34%) and median overall survival was improved by 5 months. Although this study raises questions about the relative contribution of PARP inhibition compared to an unknown mechanism of action, and that patient selection is based on triple­negative status rather than by mutational analysis, we await the results of the phase III study to see if these intriguing findings can be repro­duced. A recent update has indicated that a phase III trial of iniparib combined with gemcitabine and carboplatin in patients with triple­negative metastatic breast cancer has failed to meet prespecified criteria for significance for the co­primary end points of overall survival and progression­free survival.90The future clinical application of PARP inhibitors has yet to be determined. It is likely, however, to be depen­dent on identifying patients with BRCA1 or BRCA2 defi­ciency as a basis for personalized care. Myriad Genetics have developed a BRAC Analysis® test, which currently acts as a companion diagnostic to stratify patients in phase II clinical trials of PARP inhibitors (olaparib and veliparib).91 The companion diagnostic BRAC Analysis®, which predicts the response to PARP inhibitors, might eventually be included on the drugs label.Melanoma and BRAF V600E inhibitors Melanoma accounts for about 80% of deaths from skin cancer, with a 5­year survival rate of 15%.92 BRAF is a serine–threonine kinase that activates MAP/ERK kinase signaling (Figure 1). Approximately 60% of melanomas harbor activating mutations in BRAF,92 and most com­monly valine 600 is replaced by a glutamate residue (V600E). The specific BRAF inhibitor vemurafenib demonstrated an impressive antitumor response rate in patients identified according to the BRAF V600E muta­tion.93 Patients were selected based on presence of BRAF V600E, and in the phase I trial an 81% response rate was observed in patients with metastatic melanoma.93 The presence of the BRAF V600E mutation acts as a pre­dictive response­specific biomarker for vemurafenib, allowing patients to be subgrouped for treatment. Significantly, drug resistance frequently developed after initial responses sometimes occurring with low­grade squamous carcinomas, which was shown to reflect receptor tyrosine kinase­mediated activation or activated NRAS mediated reacti v ation of the MAPK pathway.94 Importantly, the reacti v iation of MAPK/ERK signaling predicts sensitivity to MEK inhibitors, and an additional therapeutic strategy. Upregulation of PDGFRβ or muta­tions in the NRAS gene are potential biomarkers for melanoma resistant to vemurafenib.95 Development of predictive biomarkersThe examples discussed previously provide evidence of the increasing importance of predictive biomarkers as companion diagnostics to assist the clinical application of cancer medicines. To date, the handful of biomarkers that have successfully reached the clinic and gained utility as companion diagnostics were identified mostly through retrospective analysis of clinical trial data and coinciden­tal ad hoc genetic analysis. The historical knowledge of mutations and associated molecular heterogeneity that was available, before drug development, has rarely fea­tured as an integral component of the prospective trial design. The challenge now is to exploit current tech­niques that enable predictive biomarkers to be identified in a systematic prospectively­driven fashion, allowing drug development to progress hand­in­hand with the associated biomarker, and thereby open up a new more hypothesis­based approach to developing personalized cancer therapy.A variety of different high­throughput approaches have been applied to biomarker identification, including large­scale DNA sequencing, single­nucleotide polymorphism analysis, and transcript profiling by microarray and pro­teomics.4 In general, these techniques produce correlative data that can identify genes and proteins that coincide with disease state or therapeutic response, but may be difficult to integrate with the mechanisms involved inFigure 3 | Response-specific biomarkers in cancer clinical trials. In a clinical trial ofa novel cancer drug with a typical response rate of 20% and increased survival of3 months in an unselected patient population, the average survival in the overall population (12.6 months versus 12 months) is unlikely to represent a statistically significant difference and, therefore, provides insufficient justification to support further clinical activity. A patient population tested for a predictive biomarker with a 70% PPV for response in which a stratified subgroup of 100 patients is subsequently treated with a novel cancer drug will result in an average survival of 14.1 months. This increased survival would be significantly different enough compared to the unselected population to justify further clinical development.*This example assumes a 20% response rate in an unselected population and a companion PPV diagnostic value of 70%. Survival of non-responders is 12 months and of survivors 15 months. Abbreviation: PPV, positive predictive value.。

专题05 阅读理解D篇(2024年新课标I卷) (专家评价+三年真题+满分策略+多维变式) 原卷版

专题05 阅读理解D篇(2024年新课标I卷) (专家评价+三年真题+满分策略+多维变式) 原卷版

《2024年高考英语新课标卷真题深度解析与考后提升》专题05阅读理解D篇(新课标I卷)原卷版(专家评价+全文翻译+三年真题+词汇变式+满分策略+话题变式)目录一、原题呈现P2二、答案解析P3三、专家评价P3四、全文翻译P3五、词汇变式P4(一)考纲词汇词形转换P4(二)考纲词汇识词知意P4(三)高频短语积少成多P5(四)阅读理解单句填空变式P5(五)长难句分析P6六、三年真题P7(一)2023年新课标I卷阅读理解D篇P7(二)2022年新课标I卷阅读理解D篇P8(三)2021年新课标I卷阅读理解D篇P9七、满分策略(阅读理解说明文)P10八、阅读理解变式P12 变式一:生物多样性研究、发现、进展6篇P12变式二:阅读理解D篇35题变式(科普研究建议类)6篇P20一原题呈现阅读理解D篇关键词: 说明文;人与社会;社会科学研究方法研究;生物多样性; 科学探究精神;科学素养In the race to document the species on Earth before they go extinct, researchers and citizen scientists have collected billions of records. Today, most records of biodiversity are often in the form of photos, videos, and other digital records. Though they are useful for detecting shifts in the number and variety of species in an area, a new Stanford study has found that this type of record is not perfect.“With the rise of technology it is easy for people to make observation s of different species with the aid of a mobile application,” said Barnabas Daru, who is lead author of the study and assistant professor of biology in the Stanford School of Humanities and Sciences. “These observations now outnumber the primary data that comes from physical specimens(标本), and since we are increasingly using observational data to investigate how species are responding to global change, I wanted to know: Are they usable?”Using a global dataset of 1.9 billion records of plants, insects, birds, and animals, Daru and his team tested how well these data represent actual global biodiversity patterns.“We were particularly interested in exploring the aspects of sampling that tend to bias (使有偏差) data, like the greater likelihood of a citizen scientist to take a picture of a flowering plant instead of the grass right next to it,” said Daru.Their study revealed that the large number of observation-only records did not lead to better global coverage. Moreover, these data are biased and favor certain regions, time periods, and species. This makes sense because the people who get observational biodiversity data on mobile devices are often citizen scientists recording their encounters with species in areas nearby. These data are also biased toward certain species with attractive or eye-catching features.What can we do with the imperfect datasets of biodiversity?“Quite a lot,” Daru explained. “Biodiversity apps can use our study results to inform users of oversampled areas and lead them to places – and even species – that are not w ell-sampled. To improve the quality of observational data, biodiversity apps can also encourage users to have an expert confirm the identification of their uploaded image.”32. What do we know about the records of species collected now?A. They are becoming outdated.B. They are mostly in electronic form.C. They are limited in number.D. They are used for public exhibition.33. What does Daru’s study focus on?A. Threatened species.B. Physical specimens.C. Observational data.D. Mobile applications.34. What has led to the biases according to the study?A. Mistakes in data analysis.B. Poor quality of uploaded pictures.C. Improper way of sampling.D. Unreliable data collection devices.35. What is Daru’s suggestion for biodiversity apps?A. Review data from certain areas.B. Hire experts to check the records.C. Confirm the identity of the users.D. Give guidance to citizen scientists.二答案解析三专家评价考查关键能力,促进思维品质发展2024年高考英语全国卷继续加强内容和形式创新,优化试题设问角度和方式,增强试题的开放性和灵活性,引导学生进行独立思考和判断,培养逻辑思维能力、批判思维能力和创新思维能力。

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A genetic algorithm approach for thetime-cost trade-offin PERT networksAmir Azaron *,Cahit Perkgoz,Masatoshi SakawaDepartment of Artificial Complex Systems Engineering,Graduate School of Engineering,Hiroshima University,Kagamiyama 1-4-1,Higashi-Hiroshima,Hiroshima 739-8527,Japan AbstractWe develop a multi-objective model for the time-cost trade-offproblem in PERT net-works with generalized Erlang distributions of activity durations,using a genetic algo-rithm.The mean duration of each activity is assumed to be a non-increasing function and the direct cost of each activity is assumed to be a non-decreasing function of the amount of resource allocated to it.The decision variables of the model are the allocated resource quantities.The problem is formulated as a multi-objective optimal control problem that involves four conflicting objective functions.The objective functions are the project direct cost (to be minimized),the mean of the project completion time (min),the variance of the project completion time (min),and the probability that the project completion time does not exceed a certain threshold (max).It is impossible to solve this problem optimally.Therefore,we apply a ‘‘Genetic Algorithm for Numerical Optimizations of Constrained Problems’’(GENOCOP)to solve this multi-objective problem using a goal attainment technique.Several factorial experiments are performed to identify appropriate genetic algorithm parameters that produce the best results within a given execution time in the three typical cases with different configurations.Finally,we compare the genetic algorithm results against the results of a discrete-time approxima-tion method for solving the original optimal control problem.Ó2004Elsevier Inc.All rights reserved.0096-3003/$-see front matter Ó2004Elsevier Inc.All rights reserved.doi:10.1016/j.amc.2004.10.021*Corresponding author.E-mail address:azaron@msl.sys.hiroshima-u.ac.jp (A.Azaron).Applied Mathematics and Computation 168(2005)1317–13391318 A.Azaron et al./put.168(2005)1317–1339Keywords:Project management and scheduling;Genetic algorithm;Multiple objective program-ming;Optimal control;Design of experiments1.IntroductionSince the late1950s,critical path method(CPM)techniques have become widely recognized as valuable tools for the planning and scheduling of large pro-jects.In a traditional CPM analysis,the major objective is to schedule a project assuming deterministic durations.However,project activities must be scheduled under available resources,such as crew sizes,equipment and materials.The activity duration can be looked upon as a function of resource availability. Moreover,different resource combinations have their own costs.Ultimately, the schedule needs to take account of the trade-offbetween project direct cost and project completion time.For example,using more productive equipment or hiring more workers may save time,but the project direct cost could increase.In CPM networks,activity duration is viewed either as a function of cost or as a function of resources committed to it.The well-known time-cost trade-offproblem(TCTP)in CPM networks takes the former view.In the TCTP,the objective is to determine the duration of each activity in order to achieve the minimum total direct and indirect costs of the project.Studies on TCTP have been done using various kinds of cost functions such as linear[1,2],discrete[3], convex[4,5],and concave[6].When the cost functions are arbitrary(still non-increasing),the dynamic pro-gramming(DP)approach was suggested by Robinson[7]and Elmaghraby[8]. Tavares[9]has presented a general model based on the decomposition of the project into a sequence of stages and the optimal solution can be easily computed for each practical problem as it is shown for a real case study.Weglarz[10]stud-ied this problem using optimal control theory and assumed that the processing speed of each activity at time t is a continuous,non-decreasing function of the amount of resource allocated to the activity at that instant of time.This means that time is considered as a continuous variable.Unfortunately,it seems that this approach is not applicable to networks of a reasonable size(>10).Recently,some researchers have adopted computational optimization tech-niques,such as genetic algorithms and simulated annealing to solve TCTP.Feng et al.[11]and Chua et al.[12]proposed models using genetic algorithms and the Pareto front approach to solve construction time-cost trade-offproblems.These models mainly focus on deterministic situations.However,during project implementation,many uncertain variables dynamically affect activity durations,and the costs could also change accordingly.Examples of these variables are weather,space congestion,productivity level,etc.To solve prob-lems of this kind,PERT has been developed to deal with uncertainty in the project completion time.A.Azaron et al./put.168(2005)1317–13391319PERT does not take into account the time-cost trade-off.Therefore,com-bining the aforementioned concepts to develop a time-cost trade-offmodel under uncertainty would be beneficial to scheduling engineers in forecasting a more realistic project completion time and cost.In this paper,we develop a multi-objective model for the time-cost trade-offproblem in PERT networks,using a genetic algorithm.It is assumed that the activity durations are independent random variables with generalized Erlang dis-tributions.It is also assumed that the amount of resource allocated to each activ-ity is controllable,where the mean duration of each activity is a non-increasing function of this control variable.The direct cost of each activity is also assumed to be a non-decreasing function of the amount of resource allocated to it.The problem is formulated as a multi-objective optimal control problem, where the objective functions are the project direct cost(to be minimized), the mean of the project completion time(min),its variance(min)and the prob-ability that the project completion time does not exceed a given level(max). Then,we apply the goal attainment technique,which is a variation of the goal programming technique,to solve this multi-objective problem.For the problem concerned in this paper,as a general-purpose solution method for non-linear programming problems,in order to consider the non-linearity of problems and to cope with large-scale problems,we apply the revised GENOCOP V,developed by Suzuki[13],which is a direct extension of the genetic algorithm for numerical optimizations of constrained problems (GENOCOP),proposed by Koziel and Michalewicz[14].Three factorial experiments are performed to identify appropriate genetic algorithm parameters that produce the best results within a given execution time in the three typical cases with different configurations.Moreover,an experiment in randomized block design is conducted to study the effects of three different methods of solving this problem,including the GA,on the objective function value and on the computational time.The remainder of this paper is organized in the following way.In Section2, we extend the method of Kulkarni and Adlakha[15]to analytically compute the project completion time distribution in PERT networks with generalized Erlang distributions of activity durations.Section3presents the multi-objec-tive resource allocation formulation.In Section4,we explain the revised GENOCOP V.Section5presents the computational experiments,andfinally we draw conclusions from these experiments in Section6.2.Project completion time distribution in PERT networksIn this section,we present an analytical method to compute the distribution function of the project completion time in PERT networks,or in fact the distribution function of the longest path from the source to the sink node ofa directed acyclic stochastic network,where the arc lengths or activity dura-tions are mutually independent random variables with generalized Erlang dis-tributions.To do this,we extend the technique of Kulkarni and Adlakha[15], because this method is an analytical one,simple,easy to implement on a com-puter and computationally stable.Let G=(V,A)be a PERT network with set of nodes V={v1,v2,...,v m}and set of activities A={a1,a2,...,a n}.Duration of activity a2A(T a)exhibits a generalized Erlang distribution of order n a and the infinitesimal generator matrix G a as:G a¼Àk a1k a10 (00)0Àk a2k a2 (00):::...::000...Àk anak ana 000 (00)2666666437777775:In this case,T a would be the time until absorption in the absorbing state.An Erlang distribution of order n a is a generalized Erlang distribution withk a1¼k a2¼ÁÁÁ¼k ana .When n a=1,the underlying distribution becomes expo-nential with the parameter k a1.First,we transform the original PERT network into a new one,in which all activity durations have exponential distributions.For constructing this net-work,we use the idea that if the duration of activity a is distributed according to a generalized Erlang distribution of order n a and the infinitesimal generator matrix G a,it can be decomposed to n a exponential series of arcs with theparameters k a1,k a2,...,k ana .Then,we substitute each generalized Erlang activ-ity with n a series of exponential activities with the parameters k a1,k a2,...,k ana .Now,Let G0=(V0,A0)be the transformed network,in which V0and A0rep-resent the sets of nodes and arcs of this transformed network,respectively, where the duration of each activity a2A0is exponential with parameter k a. The source and sink nodes are denoted by s and t,respectively.For a2A0, let a(a)be the starting node of arc a,and b(a)be the ending node of arc a. Definition1.Let I(v)and O(v)be the sets of arcs ending and starting at node v, respectively,which are defined as follows:IðvÞ¼f a2A0:bðaÞ¼v gðv2V0Þ,ð1ÞOðvÞ¼f a2A0:aðaÞ¼v gðv2V0Þ:ð2ÞDefinition2.If X&V0,such that s2X and t2X¼V0ÀX,then an(s,t)cut is defined as1320 A.Azaron et al./put.168(2005)1317–1339A.Azaron et al./put.168(2005)1317–13391321ðX,XÞ¼f a2A0:aðaÞ2X,bðaÞ2X g:ð3ÞAn(s,t)cutðX,XÞis called an uniformly directed cut(UDC),ifðX,XÞis empty.Example1.Before proceeding,we illustrate the material by an example. Consider the network shown in Fig.1.Clearly,(1,2)is a uniformly directed cut(UDC),because V0is divided into two disjoint subsets X and X,where s2X and t2X.The other UDCs of this network are(2,3),(1,4,6),(3,4,6)and(5,6).Definition3.Let D=E[F be a uniformly directed cut(UDC)of a network. Then,it is called an admissible2-partition,if I(b(a))X F,for a2F.To illustrate this definition,consider Example1again.As mentioned, (3,4,6)is a UDC.This cut can be divided into two subsets E and F.For exam-ple,E={4}and F={3,6}.In this case,the cut is an admissible2-partition,be-cause I(b(3))={3,4}X F and also I(b(6))={5,6}X F.However,if E={6} and F={3,4},then the cut is not an admissible2-partition,because I(b(3))={3,4}&F={3,4}.Definition4.During the project execution and at time t,each activity can be in one of the active,dormant or idle states,which are defined as follows:(i)Active.An activity is active at time t,if it is being executed at time t. (ii)Dormant.An activity is dormant at time t,if it hasfinished but there is at least one unfinished activity in I(b(a)).If an activity is dormant at time t, then its successor activities in O(b(a))cannot begin.(iii)Idle.An activity is idle at time t,if it is neither active nor dormant at time t.The sets of active and dormant activities are denoted by Y(t)and Z(t),respec-)).tively,and X(t)=(Y(t),Z(tConsider Example 1,again.If activity 3is dormant,it means that this activ-ity has finished but the next activity,i.e.5,cannot begin because activity 4is still active.Table 1,presents all admissible 2-partition cuts of this network.We use a superscript star to denote a dormant activity.All others are active.E contains all active while F includes all dormant activities.Let S denote the set of all admissible 2-partition cuts of the network,and S ¼S [fð/,/Þg .Note that X (t )=(/,/)implies that Y (t )=/and Z (t )=/,i.e.all activities are idle at time t and hence the project is completed by time t .It is proven that {X (t ),t P 0}is a continuous-time Markov process with state space S ,refer to [15]for details.As mentioned,E and F contain active and dormant activities of a UDC,respectively.When activity a finishes (with the rate of k a ),and there is at least one unfinished activity in I (b (a )),it moves from E to a new dormant activities set,i.e.to F 0.Furthermore,if by finishing this activity,its succeeding ones,O (b (a )),become active,then this set will also be included in the new E 0,while the elements of I (b (a )),which one of them belongs to E and the other ones be-long to F ,will be deleted from the particular sets.Thus,the elements of the infinitesimal generator matrix Q =[q{(E ,F ),(E 0,F 0)}],(E ,F )and ðE 0,F 0Þ2S ,are calculated as follows:q fðE ;F Þ;ðE 0;F 0Þg ¼k a if a 2E ;I ðb ða ÞÞ&F [f a g ;E 0¼E Àf a g ;F 0¼F [f a g ;ð4Þk a if a 2E ;I ðb ða ÞÞ&F [f a g ;E 0¼ðE Àf a gÞ[O ðb ða ÞÞ;F 0¼F ÀI ðb ða ÞÞ;ð5ÞÀP a 2E k a if E 0¼E ;F 0¼F ;ð6Þ0otherwise :ð7Þ8>>>>>>>>>><>>>>>>>>>>:In Example 1,if we consider E ={1,2},F (/),E 0={2,3}and F 0=(/),then E 0=(E À{1})[O (b (1)),and thus from (5),q{(E ,F ),(E 0,F 0)}=k 1.{X (t ),t P 0}is a finite-state absorbing continuous-time Markov process.Since q{(/,/),(/,/)}=0,this state would be an absorbing one and obviouslyTable 1All admissible 2-partition cuts of the example network1.(1,2) 5.(1,4*,6)9.(3*,4,6)13.(3,4*,6*)17.(/,/)2.(2,3) 6.(1,4,6*)10.(3,4*,6)14.(5,6)3.(2,3*)7.(1,4*,6*)11.(3,4,6*)15.(5*,6)4.(1,4,6)8.(3,4,6)12.(3*,4,6*)16.(5,6*)1322 A.Azaron et al./put.168(2005)1317–1339A.Azaron et al./put.168(2005)1317–13391323 the other states are transient.Furthermore,we number the states in S such that the Q matrix is an upper triangular matrix.We assume that the states are num-bered1,2,...,N¼j S j.State1is the initial state,namely(O(s),/);and state N is the absorbing state,namely(/,/).Let T represent the length of the longest path in the network,or the project completion time.Clearly,T=min{t>0:X(t)=N/X(0)=1}.Thus,T is the time until{X(t),t P0}gets absorbed in thefinal state starting from state1.Chapman–Kolmogorov backward equations can be applied to compute F(t)=P{T6t}.If we define:P iðtÞ¼P f XðtÞ¼N=Xð0Þ¼i g,i¼1,2,...,N:ð8ÞThen F(t)=P1(t).The system of differential equations for the vector P(t)=[P1(t), P2(t),...,P N(t)]T is given byP0ðtÞ¼QPðtÞ,ð9ÞPð0Þ¼½0,0,...,1 T:3.Multi-objective resource allocation problemIn this section,we develop a multi-objective model to optimally control the resources allocated to the activities in a PERT network whose activity dura-tions exhibit generalized Erlang distributions,where the mean duration of each activity is a non-increasing function and the direct cost of each activity is a non-decreasing function of the amount of resource allocated to it.We may de-crease the project direct cost,by decreasing the amount of resource allocated to the activities.However,clearly it causes the mean project completion time to be increased,because these objectives are in conflict with each other.Conse-quently,an appropriate trade-offbetween the total direct costs and the mean project completion time is required.The variance of the project completion time should also be considered in the model,because when we only focus on the mean time,the resource quantities may be non-optimal if the project com-pletion time substantially varies because of randomness.The probability that the project completion time does not exceed a certain threshold is also impor-tant in many cases and to be considered.Therefore,we have a multi-objective stochastic programming problem.The objective functions are the project direct cost(to be minimized),the mean of project completion time(min),the variance of project completion time(min), and the probability that the project completion time does not exceed a certain threshold(max).The direct cost of activity a 2A is assumed to be a non-decreasing function d a (x a )of the amount of resource x a allocated to it.Therefore,the project direct cost would be equal to P a 2A d a ðx a Þ.The mean duration of activity a 2A ,which is equal to P n a j ¼11k a j ,is assumed to be the non-increasing function g a (x a )of the amount of resource x a allocated to it.Let U a represent the amount of resource available to be allocated to the activity a ,and L a represent the minimum amount of resource required to achieve the activity a .In reality d a (x a )and g a (x a )can be estimated using linear regression.We can collect the sample paired data of d a (x a )and g a (x a )as the dependent variables,for different values of x a as the independent variables,from the previous similar activities or using the judgments of the experts in this area.Then,we can esti-mate the parameters of the relevant linear regression model.The mean and the variance of project completion time are given byE ðT Þ¼Z 1ð1ÀP 1ðt ÞÞd t ,ð10ÞVar ðT Þ¼Z10t 2P 01ðt Þd t ÀZ10tP 01ðt Þd t 2,ð11Þwhere P 01ðt Þis the density function of project completion time.The probability that the project completion time does not exceed the given threshold u isP ðT 6u Þ¼P 1ðu Þ:ð12ÞThe infinitesimal generator matrix Q would be a function of the vector k ¼½k a j ;a 2A ,j ¼1,2,...,n a T ,in the optimal control problem.Therefore,the non-linear dynamic model isP 0ðt Þ¼Q ðk ÞP ðt Þ,P i ð0Þ¼08i ¼1,2,...,N À1,P N ðt Þ¼1:ð13ÞAccordingly,the appropriate multi-objective optimal control problem isMin f 1ðx ,k Þ¼X a 2A d a ðx a Þ,Minf 2ðx ,k Þ¼Z 10ð1ÀP 1ðt ÞÞd t ,Minf 3ðx ,k Þ¼Z 10t 2P 01ðt Þd t ÀZ10tP 01ðt Þd t 2,Max f 4ðx ,k Þ¼P 1ðu Þ1324 A.Azaron et al./put.168(2005)1317–1339s :t :P 0ðt Þ¼Q ðk ÞP ðt Þ,P i ð0Þ¼08i ¼1,2,...,N À1,P N ðt Þ¼1,g a ðx a Þ¼X n a j ¼11k a j a 2A ,x a 6U aa 2A ,x a P L aa 2A ,k a j P 0a 2A ,j ¼1,2,...,n a :ð14ÞA possible approach to solving (14)to optimality is to use the Maximum Principle (see [16]for details).For simplicity,consider solving the problem with only one of the objective functions,f 2ðx ,k Þ¼R 10ð1ÀP 1ðt ÞÞd t .Clearly,x a ¼g À1a P n a j ¼11k aj for a 2A .Therefore,we can consider k as the unique control vector of the problem,and ignore the role of x =[x 1,x 2,...,x n ]T as the other independent decision vector.Consider K as the set of allowable controls consisting of all constraints except the constraints representing the dy-namic model (k 2K ),and N -vector l (t )as the adjoint vector function.Then,Hamiltonian function would beH ðl ðt Þ,P ðt Þ,k Þ¼l ðt ÞT Q ðk ÞP ðt Þþ1ÀP 1ðt Þ:ð15ÞNow,we write the adjoint equations and terminal conditions,which areÀl 0ðt ÞT ¼l ðt ÞT Q ðk Þþ½À1,0,...,0 ,l ðT ÞT ¼0,T !1:ð16ÞIf we could compute l (t )from (16),then we would be able to minimize the Hamiltonian function subject to k 2K in order to get the optimal control k *,and solve the problem optimally.Unfortunately,the adjoint equations (16)are dependent on the unknown control vector,k ,and therefore they cannot be solved directly.If we could also minimize the Hamiltonian function (15),subject to k 2K ,for an optimal control function in closed form as k *=f (P *(t ),l *(t )),then we would be able to substitute this into the state equations,P 0(t )=Q (k )ÆP (t ),P (0)=[0,0,...,1]T ,and adjoint equations (16)to get a set of differential equa-tions,which is a two-point boundary value problem.Unfortunately,we cannot obtain k *by differentiating H with respect to k ,because the minimum of H A.Azaron et al./put.168(2005)1317–13391325occurs on the boundary of K ,and consequently k *cannot be obtained in a closed form.According to these points,it is impossible to solve the optimal control prob-lem (14),optimally,even in the restricted case of a single objective problem.Relatively few optimal control problems can be solved optimally.Therefore,we apply a genetic algorithm for numerical optimizations of constrained prob-lems (revised GENOCOP V),which is fully described in Section 4,to solve this problem,using a goal attainment method.3.1.Goal attainment methodThis method requires setting up a goal and weight,b j and c j (c j P 0)for j =1,2,3,4,for the four indicated objective functions.The c j relates the relative under-attainment of the b j .For under-attainment of the goals,a smaller c j is associated with the more important objectives.c j ,j =1,2,3,4,are generally normalized so that P 4j ¼1c j ¼1.The appropriate goal attainment formulationto obtain x *isMinz s :t :Xa 2A d a ðx a ÞÀc 1z 6b 1,Z10ð1ÀP 1ðt ÞÞd t Àc 2z 6b 2,Z 10t 2P 01ðt Þd t ÀZ10tP 01ðt Þd t 2Àc 3z 6b 3,P 1ðu Þþc 4z P b 4,P 0ðt Þ¼Q ðk ÞP ðt Þ,P i ð0Þ¼08i ¼1,2,...,N À1,P N ðt Þ¼1,g a ðx a Þ¼X n a j ¼11k a j a 2A ,x a 6U aa 2A ,x a P L aa 2A ,k a j P 0a 2A ,j ¼1,2,...,n a ,z P 0:ð17Þ1326 A.Azaron et al./put.168(2005)1317–1339Lemma 1.If x *is Pareto-optimal,then there exists a c,b pair such that x *is an optimal solution to the optimization problem (17).4.A genetic algorithm for numerical optimizations of constrained problems (revised GENOCOP V)In this section,we use the revised GENOCOP V proposed as a general purpose method for solving non-linear programming problems as defined in (18).Min f ðk Þs :t :g r ðk Þ¼0,r ¼1,2,...,k 1,h r ðk Þ60,r ¼k 1þ1,k 1þ2,...,k ,L j 6k j 6U j ,j ¼1,2,...,l ,ð18Þwhere k is an l dimensional decision vector,g r (k )=0,r =1,2,...,k 1,are k 1equality constraints and h r (k )60,r =k 1+1,k 1+2,...,k ,are k Àk 1inequality constraints.These are assumed to be either linear or non-linear real-values functions.Moreover,L j and U j ,j =1,...,l ,are the lower and upper bounds of the decision variables,respectively.In order to have the same form given in (18),we reformulate the problem (17),by combining the objective functions and the state equations.We also consider a new decision vector k =[k j ;j =1,2,...,m ]T,where m ¼n þP n i ¼1n i ,instead of the original decision vectors x and k ,in the reformulated problem (19).The appropriate min–max problem is obtained as:Min f ðk Þ¼Max f z 1ðk Þ,z 2ðk Þ,z 3ðk Þ,z 4ðk Þgs :t :g r ðk Þ¼0,r ¼1,2,...,n ,L j 6k j 6U j ,j ¼1,2,...,n ,ð19Þwherez 1ðk Þ¼f 1ðx ,k ÞÀb 1c 1,z 2ðk Þ¼f 2ðx ,k ÞÀb 2c 2,z 3ðk Þ¼f 3ðx ,k ÞÀb 3c 3,z4ðkÞ¼b4Àf4ðx,kÞc4,g r ðkÞ¼g rðk rÞÀX n rj¼11k rj¼0,r¼1,2,...,n,j¼1,2,...,n randP0ðtÞ¼QðkÞPðtÞ,Pð0Þ¼½0,0,...,1 T:ð20ÞIt should be noted that in our computer program,P1(t)is obtained by solv-ing the system of differential equations(20)analytically and then the mean and the variance of project completion time are computed,numerically.The prob-lem(19)does not have the inequality constraints(h r(k)60)of problem(18). The only restriction that we have in this problem is that the elements of k vec-tor(decision variables)are selected between the given lower and upper bounds.We apply the revised GENOCOP V,developed by Suzuki[13],which is a direct extension of the genetic algorithm for numerical optimizations of con-strained problems(GENOCOP),proposed by Koziel and Michalewicz[14]. In GENOCOP V,an initial reference point is generated randomly from indi-viduals satisfying the lower and upper bounds,which is quite difficult in prac-tice.Furthermore,because a new search point is randomly generated on the line segment between a search point and a reference point,the effectiveness and speed of the search may be quite low.The proposed revised GENOCOP V overcomes these drawbacks by generating an initial reference point by min-imizing the sum of squares of the violated non-linear constraints and using a bisection method for generating a new feasible point on the line segment between a search point and a reference point.To be more explicit aboutfinding the initial reference point,for some k,Lj6 k j6U j,j=1,...,l,we use the set of violated non-linear equality constraintsI g¼f w j g wð kÞ¼0,w¼1,...,k1gð21aÞand the set of violated non-linear inequality constraintsI h¼f w j h wð kÞ>0,w¼k1þ1,...,k g:ð21bÞAn unconstrained optimization problem is formulated to minimize the sum of squares of violated non-linear constraintsMinXw2I g ðg wðkÞÞ2þXw2I hðh wðkÞÞ2ð21cÞand the optimization problem(21)is solved for obtaining one initial reference point.In the bisection method for generating a new search point,two cases are considered,in which the search points are either feasible or infeasible individuals.If search points are feasible,a new search point is generated on the line seg-ment between a search point and a reference point.If search points are infea-sible,a boundary point is found and a new point is generated on the line segment between the boundary point and a reference point.If the feasible space is not convex,the new point could be infeasible.In this case the generation of a new point is repeated if becomes feasible.putational procedures of revised GENOCOP VIn this section,the genetic algorithm for numerical optimizations of con-strained problems(revised GENOCOP V)is summarized step by step.Step0.Determine the values of the population size P,the total number of generations G,the probability of mutation P m,and the probabilityof crossover P c.Step1.Generate one or more initial reference points by minimizing the sum of squares of violated non-linear constraints.Step2.Generate the initial population consisting of P individuals.Step3.Solve the system of differential equations in(20)and compute P1(t)for each individual.The solution of the system is found as follows;first,the eigenvalues and then the related eigenvectors of the constant coef-ficient matrix Q are found.According to the eigenvectors and theeigenvalues of the system,the solution is found for each individual. Step4.Decode each individual(genotype)in the current population and cal-culate itsfitness(phenotype).Step5.Apply the mutation and crossover operations with the probabilities provided in step0.Step6.Generate the new population by applying the reproduction operator, based on the ranking selection.Step7.When the maximum number of iterations is reached,then go to step8.Otherwise,increase the generation number by1and then go to step3. Step8.Stop.putational experimentsTo investigate the performance of the proposed genetic algorithm method (revised GENOCOP V)for the time-cost trade-offproblem in PERT networks, we consider3typical small,medium and large cases with different configura-。

INTRODUCTION TO SPATIAL ECONOMETRICS USING R

INTRODUCTION TO SPATIAL ECONOMETRICS USING R

Caveats
Modifiable areal unit problem (Openshaw and Taylor, 1979)
The choice of spatial weight matrix
The link between spatial modeling and social theories
A spatial perspective better reflects the real world as people are not confined by administrative boundaries.
How Do We Analyze Spatial Data?
Exploratory spatial data analysis (ESDA):
County-level mortality data (1998-2002) Independent variables drawn from 2000 Census
Tasks:
Load necessary R packages Read the shapefile containing data Visualize the dependent variable and save it as a figure Generate spatial weight matrix using the shapefile Test spatial dependence (both global and local) Examine if a spatial perspective is better Implement spatial econometrics models Conduct model comparisons

新媒体社交媒体营销外文翻译文献

新媒体社交媒体营销外文翻译文献

文献信息:文献标题:Elements of strategic social media marketing: A holisticframework(战略性社交媒体营销要素:整体框架)国外作者:Reto Felix, Philipp A. Rauschnabel, Chris Hinsch文献出处:《Journal of Business Research》,2017,70:118-126字数统计:英文2632单词,15772字符;中文5082汉字外文文献:Elements of strategic social media marketing:A holistic frameworkAbstract Social media marketing is an integral element of 21st-centurybusiness. However, the literature on social media marketing remains fragmented and is focused on isolated issues, such as tactics for effective communication. The curr research a pplies a qualitative,theory-building a pproach to develop a strategicfour generic dimensions of strategic social m ediaframework that articulatesmarketing. Social m edia marketing scope represents a range from defenders t osocial media marketing c ulture includes the poles o f conservatism andexplorers,modernism, social media marketing structures fall between hierarchies and networks, and social m edia marketing governance ranges from autocracy t o anarchy. B yproviding a comprehensive conceptualization and definition of strategic social mediaframework that e xpands beyondmarketing, this r esearch proposes a n integrativeextant marketing theory. Furthermore, managers can apply the framework to position their organizations on these four dimensions in a manner consistent with their overa corporate mission and objectives.Key Words: Strategic social media marketing; Holistic framework; New media; Definition of social media marketing; Social media strategy; Digital marketing1.IntroductionUnderstanding the role of social media in the context of marketing is critical f both researchers and managers (e.g. Fong & Burton, 2008; Kumar, Bezawada,2013). Most existingRishika, Janakiraman, &Kannan, 2016; Schultz &Peltier,studies focus on particular issues, such as purchase behavior (Chang, Yu, & Lu, 2015 Kumar et al., 2016; Relling, S chnittka, Sattler, & Johnen, 2016), customerrelationshipmanagement (Trainor, Andzulis, R app, & Agnihotri, 2014), brandmanagement (Asmussen, Harridge-March, Occhiocupo, & Farquhar, 2013),innovation management (Gebauer, Füller, & Pezzei, 2013), and employee recruitment (Sivertzen,Nilsen, & Olafsen, 2013). W hile these s tudies detail advancements inspecialized areas of social media knowledge in a marketing and management context, extant literature does not provide a holistic framework for social media marketing the strategic level. This deficiency is surprising because both academics (Labrecqu vor dem Esche, Mathwick, Novak, & Hofacker, 2013; Schultz & Peltier, 2013; Yadav& Pavlou, 2014) and practitioners (Divol, Edelman, & Sarrazin, 2012) acknowledge new complexities accompanying these m edia and agree that r esearch into s ocialsocial mediastrategicmedia marketing n eeds to be reconceptualized.In a nutshell,marketing remains an untested user interaction paradigm (Naylor, Lamberton, & West, 2012) with little published academic research.The current article aims to address this theoretically and managerially importan research gap by exploring the following two research questions: How is strategicsocial m edia marketing defined a nd conceptualized? and What factors demandsocial media marketing s trategy?an organization'swhen constructingconsiderationSpecifically, this research attempts to define the continua on which critical strat social media marketing decisions lie and to integrate them into a holistic framewor2.MethodologyThe study e mployed a two-stage r esearch design. The first stage c onsisted ofin-depth interviews (Fontana & Frey, 1998) with seven European social m ediaexperience in socialmarketing e xperts who possess b oth national and internationalmedia marketing. Following a purposive sampling strategy (Lincoln & Guba, 1985), experts were recruited according to their job position, experience, and direct expos to social media marketing practices in real industry settings. Seeking depth rather tthe qualitative research breadth, the sample size instage 1 was commensurate with-richparadigm in which relatively small sample sizes are used to generate informationdata (Patton, 1990). A ll informants agreed t o audiotape the i nterviews (between 25and 60 min), which resulted in 117 pages of double-spaced, verbatim transcripts.procedure c onsisted of a qualitative The second stage o f the data collectionsurvey of social media marketing experts (Miles & Huberman, 1994). The survey data were used not for confirmation but as a new and independent qualitative data source with a focus on triangulating the information obtained through the depth interview and online surveys (Jack & Raturi, 2006). Respondents came from a list of 265 social media marketing experts identified through managerially focused magazines, through interviews in business magazines, or because they were mentioned as knowledgeable and experienced experts in personal communication. E-mail requests were sent to allexperts along with two reminders, which resulted in 50 returned surveys (responserate = 18.9%). Seven data s ets w ere eliminated because o f incomplete a nswers orbecause social media marketing plays a minor role in the respondents' daily work. The final sample consisted of 43 respondents (age: m = 37; SD = 9 years; 74% male; 88% European) with various backgrounds in their position and/or industry.Table 1 Summary of stage 2 informantsCompany size (employees)< 50 14 (32.6%)50–99 5 (11.6%)100–499 3 (7.0%)500–9998 (18.6%)1000–4999 6 (14.0%)5000–10,000 3 (7.0%)>10,000 4 (9.3%)Social media marketing experience (in years)Average: 6.4 years1–2 5 (11.6%)3–519 (44.2%)6–812 (27.9%)9 and more 7 (16.3%)Percentage of working time associated with social mediaAverage: 52.8%<20%7 (16.3%)20–3910 (23.3%)40–59 6 (14.0%)60–79 3 (7%)80–10015 (34.9%)n/a 2 (4.7%)Age (in years)Average: 37< 30 9 (20.9%)30–3919 (44.2%)40–4910 (23.3%)50–59 5 (11.6%)Self-reported experience in…Means aSocial Media 6.2Social Media Marketing 5.9Marketing 5.7Customer Management 4.7Advertising 5.2Communications/Public Relations 5.6How much experience do you have in the following areas? (1 = no experience at aall; 7 = highly experienced)Respondents were asked to (1) define s ocial m edia marketing, (2) discussself-selected best and worst practice examples of social media marketing, (3) discusssuccess factors and success m etrics, and (4) d escribe their ideal implementation of-selected organization. The survey employed commonsocial media marketing in a self& Mcbride, 2009), s uch as addingdesign t echniques (Smyth, Dillman, C hristian,large answer fields and asking respondents to be as specific as possible, to increrespondents' motivation to provide detail.3.FindingsFig. 1 shows the strategic social media marketing framework with its four central dimensions.Fig. 1. Strategic social media marketing framework.social media marketing s cope addresses the question whether companies First,use social media marketing p redominantly for communication with one or a fewstakeholders or comprehensively (both externally and internally) as a genuine tool f Defenders use social m edia marketing primarily a s a one-waycollaboration.rather than communication tool t o entertainconsumers or to inform stakeholders,groups. Conversely, explorers are interested in integrating employees or communityan authentic social media marketing c ollaborationinteractionsbased on reciprocalwith many different stakeholders such as clients, employees, suppliers, andgovernment agents.between conservatism, Second, social media marketing c ulture distinguishesapproach t owhich is represented by an encapsulated,mass advertisingtraditional,social media marketing, and modernism, which is characterized by a more permeable,open, and flexible social media marketing culture.Third, social media marketing structure addresses the organization and departmentalization of the social media marketing assignment in the firm. Hierarchieapproach with a clearly defined s ocial media marketingstand for a centralizedassignee. Networks represent an organizational structure in which all employees are responsible for social media marketing, and thus a dedicated social media marketing director is no longer necessary.Fourth, social m edia marketing governance refers t o how the companyestablishes rules and guidelines and how social media marketing responsibilities ar controlledin the company. The extreme position of autocracy describes a situationwith precise regulations on who in the company is allowed to interact on social medi platforms. Conversely, anarchy represents a situation without any such rules o rguidelines.The current research focuses on the extremes of each continuum, but, in general, firms likely choose (intentionally or unintentionally) a position somewhere between the poles o n each dimension. For example, c ompanies need to find a position onsocial media marketing g overnance t hat neither regulates everything employees areallowed to say nor leaves t hem without any guidance on which to base their responsibilitiesor behaviors. Fig. 1 also suggests that d ecisions on social mediageneral(e.g.,marketing s hould i ndeed b e guided by the firm's internalinfluencersvision, mission, corporate goals, corporate culture, available resources), which in should be in line with external i nfluencers (e.g., communities, competition,government regulation).4.Discussion and implicationsThis research addresses the absence of a holistic framework for strategicsocialreveals several approachesmedia marketing. A review of the marketing l iteraturemanagementregarding aspects of strategicmarketing s uch as customer r elationship(e.g., Payne & Frow, 2005) or marketing organization (Workman, Homburg, &Gruner, 1998). However, few articles address the strategic marketing of social mediaand none put forth a holistic social media marketing framework.While extant research related to social media marketing investigates social media mostly through the lens of a particular marketing problem (e.g. Fong & Burton, 2008; Kim & Ko, 2012; Kumar et al., 2016) or witha focus on customers and communication (e.g., Chang et al., 2015), t he findings of this s tudy reveal f ourgeneral social m edia marketing dimensions that firms should address when conceptualizing or managing their strategic social media marketing approach. As the findings indicate, these dimensions are interdependent, and companies should strive position themselves on the four dimensions in an integrated way, rather than treati them as isolated, independent decisions.4.1.A new definition of social media marketingThis research suggests a new definition of social media marketing: Social media-functional concept that uses social media marketing is an interdisciplinary and cross(often in combination with other communications channels) to achieve organizational goals by creating value for stakeholders. On a strategic level, social media marketi covers an organization's decisions about social media marketing scope (ranging from defenders to explorers), culture (ranging from conservatism to modernism), structurto networks), and governance (ranging from autocracy to(ranging from hierarchiesanarchy).4.2.Implications for social media marketing scopeSocial media marketing provides firms with an opportunity to use social media to with customers, employees, communities, and other stakeholdersbuild relationships(i.e., when they act as explorers). At the same time, firms may choose to view soci media as simply another c ommunications channel through which they can pushwhen they act as defenders). Though potentiallyinformation to customers (i.e.,approach does nottake advantage of thefor c ustomers, the defendercreating valuewithin the network of customers,opportunitiesfor building r eal relationshipsemployees, interest groups, the government, and other stakeholders, as propagated by modern relationshipmarketing (Payne & Frow, 2005). However, the explorerstakeholders in theapproach may require firms to redefine the role o f differentorganization.4.3.Implications for social media marketing cultureManagement and organizational behavior researchers (Zheng et al., 2010) as well as marketing academics and practitioners (Deshpandé & Farley, 2004) recognize the-financial firmimportance of culture and organizational climate for financial and non performance. The current research emphasizes t he importance o f culture for s ocialmedia marketing. Companies engaging in social media marketing must acknowledgethat stakeholders can take control of and manipulate social media content (Labrecque et al., 2013). Thus, companies should contemplate t he trade-offs between anwhich provides m oreencapsulated social media marketing c ulture (conservatism),connect a nd engageand consumers' desire tobrand constructs,control of importantwith firms displaying a more progressive, permeable culture (i.e., modernism).4.4.Implications for social media marketing structureExtant marketing research investigates how the elements of marketing should besuch as formalization,characteristics,organized a ccording t o a firm's structural(e.g.,Olson, S later, & Hult, 2005). C onsequently,and specializationcentralization,and organizationalsocial m edia marketing structure focuses on responsibilitiesemployed to configure social media marketing. Whereas social mediahierarchiesmarketing governance pertains to who can or should say what in social media, social media marketing structure focuses on who has the responsibility to post and interac in these m edia. A s the informants emphasized, firms s hould i ntegratesocial mediaThe informantsmarketing in a way that fits with their o verarching strategies.or asets o f benefits that c an emerge from either a hierarchicalidentifieddifferentnetworked structure. However, they recommended that specific decisions about who has the responsibility to interact online with customers, activists, and pundits sh be formally discussed in the organization.4.5.Implications for social media marketing governancerights, and Research on governance usually investigates the structures,responsibilities among different employees in organizations (Freeman & Reed, 1983).-discipline of corporate governance, Information technology (IT) governance, as a subfocuses on specifying which individuals have the responsibility for making decisions on the use of IT (Brown & Grant, 2005). Whereas IT governance traditionally focuses-related purposes, social media can potentially be used byon the use of IT for work(company-granted a ccess) or unofficial(personalany employee in either officialaccount access) capacities. Therefore, the applicability of IT governance research is limited u se when extended to social m edia marketing. S ome companies havedeveloped the idea of educating e mployees about the personal a nd firm-related consequences of “undesirable” social media use through social mediamarketingguidelines andguidelines(Linke &Zerfass, 2013). However, building social mediagovernance into a holistic framework for social media marketing is novel. The role o employees in promoting brands in other contexts (and thus increasing firm value) is(Morhart, Herzog, & Tomczak, 2009).well represented in the academic literatureWeber Shandwick's (2014) recent s tudy reveals a n emerging movement termed“employee activism” in which one-third of the surveyed respondentswere socialwho defended their employers and advocated f or the firm online.media activistsEmployees may be better able to understand the needs of consumers and products that can meet those needs, and they can effectively advocate and promote the firm online. These technologiesall employees to champion the firm. Forhave allowed v irtuallyNordstrom has policies to provide e mployees withexample, the fashion r etailerof social media marketingThis applicationand expectations.knowledge, d irection,governance can increase the overall social media marketing s uccess o f the firm (Nordstrom, 2015; Ross, Beath, & Sebastian, 2015).5.Limitations and future researchavenues forfruitfulSeveral limitationsto the current study s uggest p otentiallyapproach r eveals four d imensions o f strategicfuture research. First, the qualitativesocial media marketing and identifies the extreme points of each dimension. However,of differentto identify the impactfuture researchcould u se quantitative approachespositions on each of these dimensions. Research could also investigate the influenc of each dimension on firm or social media marketing p erformance. For example,studies could try to isolate the effect of each dimension on outcome variables such2013) or, m ore specifically,newconsumer–brand engagement (Schultz & Peltier,is themedia brand engagement (Hennig-Thurau et al., 2010). A second limitationover-representation of European (especially German) informants in the analyses. Prior research d iscusses cross-cultural differences in consumers' u se of social m edia(Bernoff &Li, 2008; World Newsmedia Network, 2015). Furthermore, extantresearch advocates for the adaptation of social media content to the targeted cult(Tsai &Men, 2012). T hus, caution should b ebased on differingconsumer profilestaken in extrapolating the framework to other cultural contexts. Future research mig determine w hether aspects of cultural or economic context add dimensions t o theapproaches r egardingproposed f ramework or whether they simply require differentthe four dimensions.Future research should also investigate how other characteristics, such as cultur the type of firm (e.g., B2B vs. B2C), the industry (e.g., financial services vs.“idealadvertising agency), company size, or available resources, influence a firm'ssocial media marketing. Finally,position” on each of the dimensions o f strategic-regulatory bodies (e.g.,future research could investigate the role of regulatory or selfon social media marketing g overnance a ndWord of Mouth Marketing A ssociation)how firms can create v alue and form core competencies b y superseding t hese requirements.6.ConclusionThis study sheds light on the complex nature of strategic social media marketingis too complex to be managed and executedSocial m edia marketing, in practice,-functional collaborations exclusively by a single individual or even department. Crossalong the four d imensions o f social media marketing a re necessary to successfullynavigate in this dynamic arena.中文译文:战略性社交媒体营销要素:整体框架 摘要 社交媒体营销是21世纪商业的一个组成部分。

2023年山东省高考英语真题及答案解析

2023年山东省高考英语真题及答案解析

2023年山东省高考英语真题及答案解析本试卷共12页。

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注意事项: 1. 答题前, 考生先将自己的姓名、准考证号码填写清楚, 将条形码准确粘贴在考生信息条形码粘贴区。

2. 选择题必须使用2B铅笔填涂; 非选择题必须使用0.5毫米黑色字迹的签字笔书写, 字体工整、笔迹清楚。

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第一部分听力(1-20小题)在笔试结束后进行。

第二部分阅读(共两节,满分50分)第一节(共15小题;每小题2.5分,满分37.5分)阅读下列短文,从每题所给的A、B、C、D四个选项中选出最佳选项。

ABike Rental & Guided ToursWelcome to Amsterdam, welcome to MacBike. You see much more from the seat of a bike! Cycling is the most economical, sustainable and fun way to explore the city, with its beautiful canals, parks, squares and countless lights. You can also bike along lovely landscapes outside of Amsterdam.Why MacBikeMacBike has been around for almost 30 years and is the biggest bicycle rental company in Amsterdam. With over 2,500 bikes stored in our five rental shops at strategic locations, we make sure there is always a bike available for you. We offer the newest bicycles in a wide variety, including basic bikes with foot brake (刹车), bikes with hand brake and gears (排挡), bikes with child seats, and children’s bikes.PricesGuided City ToursThe 2.5-hour tour covers the Gooyer Windmill, the Skinny Bridge, the Rijksmuseum, Heineken Brewery and much more. The tour departs from Dam Square every hour on the hour, starting at 1:00 pm every day. You can buy your ticket in a MacBike shop or book online.1. What is an advantage of MacBike?A. It gives children a discount.B. It of offers many types of bikes.C. It organizes free cycle tours.D. It has over 2,500 rental shops.2. How much do you pay for renting a bike with hand brake and three gears for two days?A. €15.75.B. €19.50.C. €22.75.D. €29.50.3. Where does the guided city tour start?A. The Gooyer, Windmill.B. The Skinny Bridge.C. Heineken Brewery.D. Dam Square.BWhen John Todd was a child, he loved to explore the woods around his house, observing how nature solved problems. A dirty stream, for example, often became clear after flowing through plants and along rocks where tiny creatures lived. When he got older, John started to wonder if this process could be used to clean up the messes people were making.After studying agriculture, medicine, and fisheries in college, John went back to observing nature and asking questions. Why can certain plants trap harmful bacteria (细菌)? Which kinds of fish can eat cancer-causing chemicals? With the right combination of animals and plants, he figured, maybe he could clean up waste the way nature did. He decided to build what he would later call an eco-machine.The task John set for himself was to remove harmful substances from some sludge (污泥). First, he constructed a series of clear fiberglass tanks connected to each other. Then he went around to local ponds and streams and brought back some plants and animals. He placed them in the tanks and waited. Little by little, these different kinds of life got used to one another and formed their own ecosystem. After a few weeks, John added the sludge.He was amazed at the results. The plants and animals in the eco-machine took the sludge as food and began to eat it! Within weeks it had all been digested, and all that was left was pure water.Over the years, John has taken on many big jobs. He developed a greenhouse — like facility that treated sewage (污水) from 1,600 homes in South Burlington. He also designed an eco-machine to clean canal water in Fuzhou, a city in southeast China.“Ecological design” is the name John gives to what he does. “Life on Earth is kind of a box of spare parts for the inventor,” he says. “You put organisms in new relationships and observe what’s happening. Then you let these new systems develop their own ways to self-repair.”4. What can we learn about John from the first two paragraphs?A. He was fond of traveling.B. He enjoyed being alone.C. He had an inquiring mind.D. He longed to be a doctor.5. Why did John put the sludge into the tanks?A. To feed the animals.B. To build an ecosystem.C. To protect the plants.D. To test the eco-machine.6. What is the author’s purpose in mentioning Fuzhou?A. To review John’s research plans.B. T o show an application of John’s idea.C. To compare John’s different jobs.D. To erase doubts about John’s invention.7. What is the basis for John’s work?A. Nature can repair itself.B. Organisms need water to survive.C. Life on Earth is diverse.D. Most tiny creatures live in groups.CThe goal of this book is to make the case for digital minimalism, including a detailed exploration of what it asks and why it works, and then to teach you how to adopt this philosophy if you decide it’s right for you.To do so, I divided the book into two parts. In part one, I describe the philosophical foundations of digital minimalism, starting with an examination of the forces that are making so many people’s digital lives increasingly intolerable, before moving on to a detailed discussion of the digital minimalism philosophy.Part one concludes by introducing my suggested method for adopting this philosophy: the digital declutter. This process requires you to step away from optional online activities for thirty days. At the end of the thirty days, you will then add back a small number of carefully chosen online activities that you believe will provide massive benefits to the things you value.In the final chapter of part one, I’ll guide you through carrying out your own digital declutter. In doing so, I’ll draw on an experiment I ran in 2018 in which over 1,600 people agreed to perform a digital declutter. You’ll hear these participants’ stories and learn what strategies worked well for them, and what traps they encountered that you should avoid.The second part of this book takes a closer look at some ideas that will help you cultivate (培养) a sustainable digital minimalism lifestyle. In these chapters, I examine issues such as the importance of solitude (独处) and the necessity of cultivating high-quality leisure to replace the time most now spend on mindless device use. Each chapter concludes with a collection of practices, which are designed to help you act on the big ideas of the chapter. You can view these practices as a toolbox meant to aid your efforts to build a minimalist lifestyle that words for your particular circumstances.8. What is the book aimed at?A. Teaching critical thinking skills.B. Advocating a simple digital lifestyle.C. Solving philosophical problems.D. Promoting the use of a digital device.9. What does the underlined word “declutter” in paragraph 3 mean?A. Clear-up.B. Add-on.C. Check-in.D.Take-over.10. What is presented in the final chapter of part one?A. Theoretical models.B. Statistical methods.C. Practical examples.D. Historical analyses.11. What does the author suggest readers do with the practices offered in part two?A. Use them as needed.B. Recommend them to friends.C. Evaluate their effects.D. Identify the ideas behind them.DOn March 7, 1907, the English statistician Francis Galton published a paper which illustrated what has come to be known as the “wisdom of crowds” effect. The experiment of estimation he conducted showed that in some cases, the average of a large number of independent estimates could be quite accurate.This effect capitalizes on the fact that when people make errors, those errors aren’t always the same. Some people will tend to overestimate, and some to underestimate. When enough of these errors are averaged together, they cancel each other out, resulting in a more accurate estimate. If people are similar and tend to make the same errors, then their errors won’t cancel each other out. In more technical terms, the wisdom of crowds requires that people’s estima tes be independent. If for whatever reasons, people’s errors become correlated or dependent, the accuracy of the estimate will go down.But a new study led by Joaquin Navajas offered an interesting twist (转折) on this classic phenomenon. The key finding of the study was that when crowds were further divided into smaller groups that were allowed to have a discussion, the averages from these groups were more accurate than those from an equal number of independent individuals. For instance, the average obtained from the estimates of four discussion groups of five was significantly more accurate than the average obtained from 20 independent individuals.In a follow-up study with 100 university students, the researchers tried to get a better sense of what the group members actually did in their discussion. Did they tend to go with those most confident about their estimates? Did they follow those least willing to change their minds? This happened some of the time, but it wasn’t the dominant response. Most frequently, the groups reported that they “shared arguments and reasoned together.” Somehow, these arguments and reasoning resulted in a global reduction in error. Although the studies led by Navajas have limitations and many questions remain the potential implications for group discussion and decision-making are enormous.12. What is paragraph 2 of the text mainly about?A. The methods of estimation.B. The underlying logic of the effect.C. The causes of people’s errors.D. The design of Galton’s experiment.13. Na vajas’ study found that the average accuracy could increase even if ________.A. the crowds were relatively smallB. there were occasional underestimatesC. individuals did not communicateD. estimates were not fully independent14. What did the follow-up study focus on?A. The size of the groups.B. The dominant members.C. The discussion process.D. The individual estimates.15. What is the author’s attitude toward Navajas’ studies?A. Unclear.B. Dismissive.C. Doubtful.D. Approving.第二节(共5小题;每小题2.5分,满分12.5分)阅读下面短文,从短文后的选项中选出可以填入空白处的最佳选项。

意大利圣拉斐尔科学研究所招聘博士后

意大利圣拉斐尔科学研究所招聘博士后

意⼤利圣拉斐尔科学研究所招聘博⼠后A full-time post-doctoral position is available in the Laboratory of Gene Expression and Muscular Dystrophy, Stem Cell Research Institute, DIBIT-hSR, Milano, Italy.The general focus of the group centers on the definition of the molecular pathogenesis of facioscapulohumeral muscular dystrophy (FSHD). Current projects include analysis of epigenetic mechanisms regulating FSHD critical region gene expression and functional studies on the FSHD mouse model.The laboratory is strategically located within the scientific environment of the San Raffaele Biomedical Area. There are ample opportunities to interact with other laboratories with interests in regulation of gene expression and disease. The Stem Cell Research Institute has a strong emphasis on development of novel therapeutic approaches for muscular dystrophy.Representative publications:1. Gabellini et al. 2006. Facioscapulohumeral Muscular Dystrophy in Transgenic Mice by Over-Expression of FRG1. Nature 439:973-977.2. Gabellini D et al. 2002. Inappropriate gene activation in FSHD: a repressor complex binds a chromosomal repeat deleted in dystrophic muscle. Cell 110:339-348.Candidates with strong background in molecular biology, genetics, or biochemistry are encouraged to apply. Expertise in mouse biology using both in vivo and in vitro approaches is highly desirable. Applicants should have demonstrated scientific productivity, good inter-personal and communication skills, and be able to conduct independent research.Interested candidates should send enquiries, curriculum vitae, containing information about education, experience and publications, and a list of three individuals that can serve as references by e-mail to:Davide Gabellini, Ph. D.Stem Cell Research InstituteDIBIT-hSR 2A3-room 49AVia Olgettina 5820132 MilanoITALYPhone +39.02.2643.5934Fax +39.02.2643.4621*****************************。

英语托福试题及答案

英语托福试题及答案

英语托福试题及答案一、听力部分1. 问题:What is the main topic of the lecture?答案:The main topic of the lecture is the impact of industrialization on the environment.2. 问题:According to the professor, what is the primarycause of air pollution?答案:The primary cause of air pollution, according to the professor, is the burning of fossil fuels.3. 问题:What is the student's suggestion to reduce pollution?答案:The student suggests using renewable energy sourcesto reduce pollution.二、阅读部分1. 问题:What does the author argue about the role of technology in education?答案:The author argues that technology has the potentialto enhance learning experiences but also emphasizes the importance of its proper integration into the curriculum.2. 问题:What evidence does the author provide to support the benefits of technology in education?答案:The author provides evidence such as increasedstudent engagement, access to a wider range of resources, and the ability to personalize learning.3. 问题:What is the author's view on the challenges of integrating technology into education?答案:The author believes that challenges include the need for teacher training, the digital divide, and the risk of distraction.三、口语部分1. 问题:Describe a memorable event from your childhood.答案:One memorable event from my childhood was my first visit to a zoo, where I was amazed by the variety of animals and learned about their habitats.2. 问题:Why do you think it is important to learn a second language?答案:Learning a second language is important because it opens up opportunities for communication, broadens cultural understanding, and enhances cognitive abilities.3. 问题:What are some ways to improve your English speaking skills?答案:Some ways to improve English speaking skills include practicing with native speakers, joining language exchange groups, and using language learning apps.四、写作部分1. 问题:Do you agree or disagree with the following statement? University education should be free for all students.答案:[Your response should be a well-organized essay that includes an introduction, body paragraphs with supporting arguments, and a conclusion.]2. 问题:Some people believe that the government should spend more on art and culture, while others think that this money should be used for other public services. Discuss both views and give your opinion.答案:[Your response should be a well-organized essay that presents the arguments for both views, provides your own opinion, and includes a conclusion.]3. 问题:Describe a person who has had a significant influence on your life and explain why this person is important to you.答案:[Your response should be a descriptive essay that outlines the person's characteristics, the impact they have had on you, and the reasons for their significance.]。

杰尼奥公司的Raman光谱仪使用培训课程说明书

杰尼奥公司的Raman光谱仪使用培训课程说明书

6Who should attendFrom Monday 9 am to Wednesday 5:30 pmDates: February 11-13, 2019 May 13-15, 2019 June 24-26, 2019 October 7-9, 2019November 18-20, 2019Users of HORIBA Scientific Raman spectrometers • A cquire theoretical and practical knowledge on Raman spectrometers • L earn how to use the software • L earn methodology for method development and major analytical parameters • H ow to set up an analytical strategy with an unknown sample • H ow to interpret results• L earn how to follow the performances of theRaman spectrometer over the time.Day 1• The theory of the Raman principle • R aman Instrumentation • P ractical session – System and software presentation, Acquisition Parameters: - L abSpec 6 presentation and environment: useraccounts, file handling, display of data, basic functions - S et up of acquisition parameters and singlespectra measurement - Templates & ReportsDay 2• Analysis of Raman spectra • P ractical session: Raman spectrum measurement and Database Search - O ptimization of the parameters: how to chosethe laser, the grating, the confocal hole, the laser power- How to use the polarization options - Library Search using KnowItAll software - How to create databasesRaman imaging • H ow to make a Raman image (1D, 2D and 3D) • D ata evaluation: cursors, CLS fitting, peakfitting•Image rendering, 3D datasets •Fast mapping using SWIFT XSDay 3Data processing• Processing on single spectra and datasets • Baseline correction • Smoothing • Normalization• Spectra subtraction, averaging • Data reduction • Methods• Practical exercisesCustomer samples: Bring your own samples!Duration: 3 daysReference: RAM1Raman Microscopy for Beginners7Acquire technical skills on DuoScan, Ultra Low Frequency (ULF), Particle Finder or TERS.Users of HORIBA Scientific Raman spectrometers who already understand the fundamentals of Raman spectroscopy and know how to use HORIBA Raman system and LabSpec Software. It is advised to participate in the basic Raman training first (RAM1).Introduction to DuoScan• Principle and hardwareDuoScan Macrospot• Practical examplesDuoScan MacroMapping• Practical examplesDuoScan Stepping Mode• Practical examplesCustomer samples: Bring your own samples!Presentation of the ULF kit• Principle and requirements • Application examplesInstallation of the ULF kitIntroduction to Particle Finder• Principle and requirementsPractical session• Demo with known sample• Customer samples: Bring your own samples!Practical session• Demo with known samplesCustomers samples: Bring your own samples! Presentation of the TERS technique• Principle and requirements • Application examplesDemo TERS• Presentation of the different tips and SPM modes • Laser alignment on the tip • T ERS spectra and TERS imaging on known samplesPractical session• Hands-on on demo samples (AFM mode)• Laser alignment on the tip • T ERS spectra and TERS imaging on known samplesRaman Options: DuoScan, Ultra Low Frequency, Particle Finder, TERS8Users of HORIBA Scientific Raman spectrometers who already understand the fundamentals of Raman spectroscopy and know how to use HORIBA Raman system and labSpec Software. It is adviced to participate in the basic Raman training first.Who should attendDates: February 14, 2019 June 27, 2019November 21, 2019Duration: 1 dayReference: RAM2From 9 am to 5:30 pm• Acquire theoretical and practical knowledge on SERS (Surface Enhanced Raman Spectroscopy)• Know how to select your substrate • Interpret resultsRaman SERSIntroduction to SERSPresentation of the SERS technique • Introduction: Why SERS?• What is SERS?• Surface Enhanced Raman basics • SERS substratesIntroduction to the SERS applications• Examples of SERS applications • Practical advice • SERS limitsDemo on known samplesCustomer samples: Bring your own samples!Raman Multivariate Analysis9Users of HORIBA Scientific Raman spectrometerswho already understand the fundamentals of Ramanspectroscopy and know how to use HORIBA Ramansystem and LabSpec Software. It is advised toparticipate in the basic Raman training first (RAM1).• Understand the Multivariate Analysis module• Learn how to use Multivariate Analysis for data treatment• Perform real case examples of data analysis on demo and customer dataIntroduction to Multivariate Analysis• Univariate vs. Multivariate analysis• Introduction to the main algorithms: decomposition (PCA and MCR), classification and quantification (PLS)Practical work on known datasets (mapping)• CLS, PCA, MCRIntroduction to classification• HCA, k-means• Demo with known datasetsIntroduction to Solo+MIA• Presentation of Solo+MIA Array• Demo with known datasetsData evaluation: cursors, CLS fitting, peak fitting• Fast mapping using SWIFT XSObjective: Being able to select the good parameters for Raman imaging and to perform data processScanning Probe Microscopy (SPM)• Instrumentation• T he different modes (AFM, STM, Tuning Fork) and signals (Topography, Phase, KPFM, C-AFM, MFM,PFM)Practical session• Tips and sample installation• Molecular resolution in AFM tapping mode• M easurements in AC mode, contact mode, I-top mode, KPFM• P resentation of the dedicated tips and additional equipment• O bjective: Being able to use the main AFM modes and optimize the parametersimaging)Practical session• Hands-on on demo samples (AFM mode)• Laser alignment on the tip• T ERS spectra and TERS imaging on known sample Day 3TERS Hands-on• T ERS measurements, from AFM-TERS tip installation to TERS mapping.• TERS measurements on end users samples.• Bring your own samples!28Practical informationCourses range from basic to advanced levels and are taught by application experts. The theoretical sessions aim to provide a thorough background in the basic principles and techniques. The practical sessions are directed at giving you hands-on experience and instructions concerning the use of your instrument, data analysis and software. We encourage users to raise any issues specific to their application. At the end of each course a certificate of participation is awarded.Standard, customized and on-site training courses are available in France, G ermany, USA and also at your location.Dates mentionned here are only available for HORIBA France training center.RegistrationFill in the form and:• Emailitto:***********************• Or Fax it to: +33 (0)1 69 09 07 21• More information: Tel: +33 (0)1 69 74 72 00General InformationThe invoice is sent at the end of the training.A certificate of participation is also given at the end of the training.We can help you book hotel accommodations. Following your registration, you will receive a package including training details and course venue map. We will help with invitation letters for visas, but HORIBA FRANCE is not responsible for any visa refusal. PricingRefreshments, lunches during training and handbook are included.Hotel transportation, accommodation and evening meals are not included.LocationDepending on the technique, there are three locations: Longjumeau (France, 20 km from Paris), Palaiseau (France, 26 km from Paris), Villeneuve d’Ascq (France 220 km from Paris) or at your facility for on-site training courses. Training courses can also take place in subsidiaries in Germany or in the USA.Access to HORIBA FRANCE, Longjumeau HORIBA FRANCE SAS16 - 18 rue du canal91165 Longjumeau - FRANCEDepending on your means of transport, some useful information:- if you are arriving by car, we are situated near the highways A6 and A10 and the main road N20- if you are arriving by plane or train, you can take the train RER B or RER C that will take you not far from our offices. (Around 15 €, 150 € by taxi from Charles de Gaulle airport, 50 € from Orly airport).We remain at your disposal for any information to access to your training place. You can also have a look at our web site at the following link:/scientific/contact-us/france/visi-tors-guide/Access to HORIBA FRANCE, Palaiseau HORIBA FRANCE SASPassage Jobin Yvon, Avenue de la Vauve,91120 Palaiseau - FRANCEFrom Roissy Charles de Gaulle Airport By Train • T ake the train called RER B (direction Saint RemyLes Chevreuse) and stop at Massy-Palaiseaustation• A t Massy-Palaiseau station, take the Bus 91-06C or 91-10 and stop at Fresnel• T he company is a 5 minute walk from the station,on your left, turn around the traffic circle and youwill see the HORIBA building29 Practical InformationAround 150 € by taxi from Charles de Gaulle airport. From Orly Airport By Train• A t Orly airport, take the ORLYVAL, which is ametro line that links the Orly airport to the AntonyRER station• A t Antony station, take the RER B (direction StRemy Les Chevreuse) and stops at Massy-Palai-seau station• A t Massy-Palaiseau station, take the Bus 91-06C, 91-06 B or 91-10 stop at Fresnel• T he company is 5 minutes walk from the station,on your left, turn around the traffic circle and youwill see the HORIBA building• O r at Orly take the Bus 91-10 stop at Fresnel.The company is 5 minutes walk from the station,on your left, turn around the traffic circle and youwill see the HORIBA building. We remain at yourdisposal for any information to access to your trainingplace. You can also have a look at our web site at thefollowing link:/scientific/contact-us/france/visi-tors-guide/Around 50 € by taxi from Orly airport.Access to HORIBA FRANCE, Villeneuve d’Ascq HORIBA Jobin Yvon SAS231 rue de Lille,59650 Villeneuve d’Ascq - FRANCEBy Road from ParisWhen entering Lille, after the exit «Aéroport de Lequin», take the direction «Bruxelles, Gand, Roubaix». Immmediatly take the direction «Gand / Roubaix» (N227) and No «Bruxelles» (A27) Nor «Valenciennes» (A23).You will then arrive on the ringroad around Villeneuve d’Ascq. Take the third exit «Pont de Bois».At the traffic light turn right and follow the road around, (the road will bend left then right). About 20m further on you will see the company on the right hand side where you can enter the car park.By Road from Belgium (GAND - GENT)Once in France, follow the motorway towards Lille. After «Tourcoing / Marcq-en-Baroeul», follow on the right hand side for Villeneuve d’Ascq. Take the exit «Flers Chateau» (This is marked exit 6 and later exit 5 - but it is the same exit). (You will now be following a road parallel to the mo-torway) Stay in the middle lane and go past two sets of traffic lights; at the third set of lighte, move into the left hand lane to turn under the motorway.At the traffic lights under the motorway go straight, (the road shall bend left then right). About 20 m further you shall see the company on the right hand side where you can enter the car park.AeroplaneFrom the airport Charles de Gaulle take the direction ‘Ter-minal 2’ which is also marked TGV (high speed train); where you can take the train to ‘Lille Europe’.Train - SNCFThere are two train stations in Lille - Lille Europe or Lille Flandres. Once you have arrived at the station in Lille you can take a taxi for HORIBA Jobin Yvon S.A.S., or you can take the underground. Please note both train stations have stations for the underground.Follow the signs:1. From the station «Lille Flandres», take line 1, direction «4 Cantons» and get off at the station «Pont de bois».2. From the station «Lille Europe», take line 2, direction «St Philibert» and get off at the following station «Gare Lille Flandres» then take line 1, direction «4 Cantons» and get off at the station «Pont de Bois».BusBus n°43, direction «Hôtel de Ville de Villeneuve d’Ascq», arrêt «Baudoin IX».InformationRegistration: Fill inthe form and send it back by FAX or Email four weeks before beginning of the training.Registration fees: the registration fees include the training courses and documentation. Hotel, transportation and living expenses are not included except lunches which are taken in the HORIBA Scientific Restaurant during the training.Your contact: HORIBA FRANCE SAS, 16-18 rue du Canal, 91165 Longjumeau, FRANCE Tel: + 33 1 64 74 72 00Fax: + 33 1 69 09 07 21E-Mail:***********************Siret Number: 837 150 366 00024Certified ISO 14001 in 2009, HORIBA Scientific is engaged in the monitoring of the environmental impact of its activitiesduring the development, manufacture, sales, installation and service of scientific instruments and optical components. Trainingcourses include safety and environmental precautions for the use of the instrumentsHORIBA Scientific continues contributing to the preservation of theglobal environment through analysis and measuring technologymentisnotcontractuallybindingunderanycircumstances-PrintedinFrance-©HORIBAJobinYvon1/219。

信息化视域下现代教育技术理论与实际研究

信息化视域下现代教育技术理论与实际研究

创新教育207①作者简介:高翔(1984—),女,汉族,硕士,助教,研究方向为教育技术在教学中的应用。

DOI:10.16660/ki.1674-098X.2010-5640-1038信息化视域下现代教育技术理论与实际研究①高翔(郑州幼儿师范高等专科学校 河南郑州 450099)摘 要:信息化视域下的现代教育技术,是集现代教育与信息技术为一体的综合性教学模式,是为改革教育教学,提高教学质量,优化教育结构而提出的。

本文以信息化视域下现代教育技术的理论与实际为核心,简要阐述现代教育技术的概念界定与应用定义,探索现代教育技术的应用特点与结构要素。

然后以实现现代教育技术在教育教学活动中的有效应用,发挥现代教育技术价值与作用为目的,分别为其提供两点有效对策,以供参考。

关键词:信息化 现代教育技术 理论 实际中图分类号:G641 文献标识码:A 文章编号:1674-098X(2021)01(a)-0207-03Theoretical and Practical Research on Modern EducationalTechnology from the Perspective of InformatizationGAO Xiang(Zhengzhou Preschool Education College, Zhengzhou, Henan Province, 450099 China)Abstract: The modern educational technology in the perspective of informatization is a comprehensive teaching mode integrating modern education and information technology. It is proposed to reform education, improve teaching quality and optimize educational structure. Based on the theory and practice of modern educational technology from the perspective of information technology, this paper brief ly expounds the concept definition and application definition of modern educational technology, and explores the application characteristics and structural elements of modern educational technology. Then, in order to realize the effective application of modern educational technology in education and teaching activities and give full play to the value and role of modern educational technology, two effective countermeasures are provided for reference.Key Words: Informatization; Modern educational technology; Theory; Practice信息化视域下,我国教育领域面临着被技术异化的困境,教育问题不断涌现。

teachingmethodology

teachingmethodology

TitleCompare, contrast and critically evaluate the main methods and approaches that have been used in English language teaching, comment on the extant to which each of these can be used in your future teaching practice.IntroductionTeaching methodology in EFL(English as a foreign language), which has developed for hundred years, is handed down and affects us in today’s teaching practice. In the main body, four particular teaching methods are analyzed and evaluated, especially their strengths and weakness in the real teaching practice. Also, their respective significance to EFL development can not be overlooked. Moreover, many other methods and approaches except the foregoing are briefly listed. Above all, the ultimate goal of this writing is to enrich my knowledge about language teaching and absorb what is refined in each method, which will possibly be applied to my future teaching practice.1. Generally, teaching method is a way of teaching a language which is based on systematic principles and procedures. Or we can simply regard it as an application of views on how a language is best taught and learned. Following methods were adopted in different times in order to achieve the teaching goals.2. Grammar-translation Method2.1 BackgroundThis method sprung up in the mid 18th century and reached its height around the 19th century, which was the time when classical Latin and Greek were widely taught for th ere’s a great need to read the literature. Not until the late 19th century, the time when travel became popular and conversationin English became necessary, did the GTM come under criticism.2.2 Objectives●Because of its great emphasis on understanding and appreciating foreign language literature, reading and writing are the major focuses.●Through the study of grammar, students tend to become more aware of correctness and accuracy of the sentence.2.3 Techniques●Students have to intentionally memorize rules, vocabulary and meaning. Examples will be applied frequently to illustrate and explain the rules.●Plenty of translation exercises are involved in class. Mother language is applied in class instead of the target language●Vocabulary is obtained from the text reading. Moreover, lists of vocabulary items are presented with their equivalent translation.2.4 Characteristics●Class is organized mainly in students’ mother tongue.●Grammar rules memorizing is the basic and the most important means in language learning. Also, those rules will be elaborately illustrated and explained through the text reading and sentence translation.●Almost no attention is paid to the oral and listening skills. Pronunciation is overlooked in this method2.5Assessment (strengths)●Systematic learning in reading and writing is achieved throughGMT for its great focus on rules learning.●Correctness and high-standard accuracy are presented in students’ reading and writing.●Problem-solving situation and translation-based tasks are built to activate students’ initiative s2.6Assessment (drawbacks)●Students can never get rid of the influence of their mother tongue.●Tremendous memorization is prescribed while language creativity is smothered.●In most situations, learners can only memorize one or two meanings of each word. The result is that they feel confused if the word shows up in a third meaning, which will hinders them from fully understanding the text.3. Direct Method3.1 BackgroundDirect Method can be regarded as the outcome of the lang uage teaching reforms taken from 1850-1900. For the GTM was attacked as outdated and failing to meet the needs at that time, reformers intended to build up a new method, and the DM was the invention. Linguists like M. Berlitz and F. Gonin were the main contributors.●Moreover, the revitalization of the phonetics and the founding of IPA also gave impetus to the emergence of the DM.3.2 Objectives●Unlike GMT, Direct Method puts the speaking part precedes other language skills. Oral communication is emphasized.●Target language is adopted during the whole teaching. First language translation is avoided in order to set the students free from mother tongue and develop students’ ability to think in the target language.3.3 Techniques●“Oral communication skills were built up in a carefully graded progression organized around question-and-answer exchanges between teachers and studen ts in small, intensive classes.”(By Richards and Rodgers, 1986). Besides, Class exercises like dictation, Q&A, narrative, imitation and free composition are involved.●W ords are explained in target language and demonstrations. Grammar rules are not listed by teachers but found by students through their speaking, listening and reading.●Utterances and pronunciation are stressed, and students’ w rong pronunciation will be immediately pointed out and corrected.3.4 Characteristics●Only target language is involved in class, and learners are supposed to fully immerse themselves into the environment and think in the target way.●Conversations in realistic everyday situation are prescribed for thelearners instead of the book reading and literature appreciating.3.5 Assessment (strengths)●Through total immersion in the target language, learners’ ability to think in the target language will be developed.●Pronunciation and utterance are learned.●Learners’ initiatives are encouraged, and effective teaching is achieved through the plenty of demonstrations and involved imitations.3.6Assessment (drawbacks)●Absolute avoidance of the first language sometimes troubles the teaching, for it’s difficult for teachers to explain everything mainly by paraphrasing and demonstrating, especially some abstract concepts.●Lack of translation and grammar explanation also lead to the mistakes in students’ speaking.●Overemphasized on the similarities between the natural first language acquisition and the second language learning.4. Audio-Lingual Method4.1 BackgroundALM, known as the Army Method, had its origins during the W orld W ar II which created the need to oral communication in English. Besides, the ALM arose out of the theory proposed by behaviorist psychologists like B.F Skinner, saying that all behavior (including language) was learnt through repetition and positive or negative reinforcement. In a simple word,language learning is through habit formation. Later in the late 1950s, the method fell from popularity because its theoretical basis was questioned by some linguists. Among which, Chomsky was the most famous one for his review of B.F Skinner's Verbal Behavior in 1959. Despite of all the criticisms it gained in the past several decades, ALM continues to be used today.4.2 Objectives●Four language skills are emphasized, especially listening and speaking. Students are expected to gain their communicative skills through imitation and repetition of the presented dialogues.●Without much explanation in mother tongue, grammar rules are not obtained through memorization, but by examples.4.3 Techniques●Dialogues and pattern drills are involved in class. Learners are told to imitate the dialogues and memorize the drills presented in the dialogues.●Repetition is the most important strategy. Students are asked to repeat the teacher’s sentence in order to reinforce their memorization.●False utterance will be corrected right in the spot. Repetition also applied to pronounce some unaccustomed phonemes for some particular learners.●Modern means like the tapes and video are used as aids. Correct pronunciation, rules and culture are presented through them.4.4 Characteristics●Learners are asked to mimicry, repetition and memorization all the time. Sentence structure patterns are learned through repetitive drills.●Grammar and vocabulary are obtained through the regular dialogues and context the learners memorized.●Reference to mother tongue is not permitted and native-speaker-like model is set up.●Tapes and visual aids provide an easy way for learners to access to the correct utterance.4.5 Assessment (strengths)●Compare to other methods, this method realizes both input and output. Students listen and learn, and then they memorize it and speak it out.●Through the long-term memorization, students are able to use the simple and basic sentence structures correctly. Simple grammar knowledge can be taught through the habit formation.4.6 Assessment (drawbacks)●Because of the long-term imitation and repetition, students are not able to create their own sentence or anything spontaneous. Also, they can not adapt themselves to natural speech situations.●Teachers tend to overuse the tapes and visual materials. Boredom and mindlessness are engendered among learners soon.5. Communicative Approach5.1 Background●This approach has its origins in 1960s, and became extremely popular in the 1970s when many experts, represented by Munby(1978) and Wilkins(1979), felt dissatisfied with the traditional language teaching methods like the Grammar-translation method and the audio-lingual method. Teaching communication ability at that time has become an irreversible trend in language teaching.5.2 Objectives●Students are expected to able to communicate with other people in appropriate language.●“Communicative competence” refers to the ability not only to apply the grammatical rules of language in order to form grammatically correct sentences but also to know when and where to use these sentences and to whom. (Hymes) In this method, grammar is not stressed, instead, the students are carefully taught to choose the appropriate words and sentences according to the different situations.●After the course, students are able to maintain the conversation despite the fact that their language knowledge is limited.5.3 Techniques●Communicative activities are set for learners such as role plays, problem-solving situations and designed games.●Teachers are expected to not just organize and manage the activities but sometimes take part in it in order to activate the class.●Group discussing is manipulated to ensure that every individual hasa chance to speak and interact with other group members.5.4Characteristics●W e can simply regard it as a learner-centered form of teaching.Unlike other traditional methods, in this approach, teachers function as facilitators while students are the main contributor to the class.●Authentic materials and real communication situations are used.●Emphasis is put on learning to communicate in target language.●Students’ personal emotions and experiences are concerned. They are encouraged to express their feelings in class.5.5 Assessment (strengths)●More appropriateness and accurate communications are presented in this method.●Learners are greatly activated by the classroom activities, which eventually results in effectiveness in the teaching process.5.6 Assessment (drawbacks)●Because of its randomness and great focus on fluency, learners tend to use the target language informally and sometimes incorrectly.●Without the basic knowledge of rules and vocabulary, it’s hard for students to achieve progress and become higher-level learners.6.In the long history of EFL development, there’re still many other methods and approaches, besides the foregoing ones, have contributed a lot to the language teaching development. After a shallow study of these methodologies, some simple conclusions are formed.6.1 Total Physical Response, a method claims that teachers teach words and expressions through body motions. TPR is supposed to be effective in simple teaching, but difficult to be used in complex language.6.2 Suggestopidia, which aims to speed up the teaching process by encouraging students and cultivating their confidence.6.3 Reading Method. In this method, learners are taught through foreign materials reading, with direct comprehension of meaning.6.4 Task-based Method. Just as its name implies, tasks are contributed to every students. Students learn the language through the tasks accomplishing.7. Personal opinions on application of these methods.After analyzing all the mentioned methods above, I really appreciate some of them, especially the GMT and the Communicative Method, and I intend to apply them into my future teaching. I try to analyze the application of these methods in two ways.7.1 Choose the right methods according to different target learners.Different people from different ages have different intellectual features. Children can recite poems fluently despite that they can not understand them.They are willing to open their mouths to imitate and speak. For them, Communicative Method should be greatly applied in class in order to interest them. Adults and high-level learners are more enthusiastic in learning sentence structures and grammar knowledge. So, explanations for grammar rules and advanced vocabulary should be included in teaching them.7.2 Choose the right methods according to the different phases of student’s learning.In the beginning phase of learning, students lack of vocabulary and basic knowledge, so it is not reasonable to explain knowledge in target language. Grammar-translation is necessary in this phase because it can be clearer to the beginners. Also, Total Physical Response can be very effective because of its vivid body motions for the children. In the middle rank of learning, students already gain the basic knowledge of four language skills. In this phase, Communicative Method can be effective to consolidate their knowledge. Task-based method can sometimes be a facilitator for students in this phase to activate their initiatives.All and all, effective teaching is not achieving through one or two methods. In future teaching, knowing how to combine different methods to realize different goals is the biggest theme of my future learning in teaching.ReferencesDavid Nunan, 1992, Research Methods in Language Learning, Cambridge University PressPaul Davies, 2000, Success in English Teaching,Oxford University Press王渭刚,《浅析外语教学中几种主要的教学法》,渭南师范学院外语系章兼中,《国外外语教学主要流派》,华东师范大学出版社。

Advanced Techniques for GA-based sequential ATPGs

Advanced Techniques for GA-based sequential ATPGs

Advanced Techniques for GA-based sequential ATPGsF. Corno, P. Prinetto, M. Rebaudengo, M. Sonza Reorda R. MoscaPolitecnico di Torino CSP (Centro Supercalcolo Piemonte) Torino, Italy Torino, ItalyAbstract*Genetic Algorithms have been recently investigated as an efficient approach to test generation for synchro-nous sequential circuits. In this paper we propose a set of techniques which significantly improves the per-formance of the GA-based ATPG algorithm proposed in [PRSR94]: in particular, the new techniques en-hance the capability of the algorithm in terms of test length minimization and fault excitation. We report some experimental results gathered with a prototypical tool and show that a well-tuned GA-based ATPG is generally superior to both symbolic and topological ones in terms of achieved Fault Coverage and required CPU time.1. IntroductionDifferent approaches have been proposed to solve the problem of Automatic Test Pattern Generation for Synchronous Sequential circuits.The topological approach [NiPa91] is based on ex-tending to sequential circuits the branch and bound techniques developed for combinational circuits by adopting the Huffman’s Iterative Array Model. The method’s effectiveness heavily relies on the heuristics adopted to guide the search; the approach uses a com-plete, but often fails when applied to large circuits, where the search space is excessively large to explore.The symbolic approach [CHSo93] exploits tech-niques for Boolean function representation and ma-nipulation which were initially developed for formal verification; this approach is based on a complete al-gorithm, too, and is very effective when small- and medium-sized circuits are considered. Unfortunately, it is completely unapplicable when circuits with more than some tens of Flip-Flops are dealt with. This greatly limits its usefulness in real practice.Finally, the simulation-based approach [ACAg88] consists in generating random sequences, simulating them, and then modifying their characteristics in order to increase the obtained fault coverage. In the last few * This work has been partially supported by European Union through the ESPRIT PCI project #9452 94 204 70 PETREL. Contact address: Paolo Prinetto, Dipartimento di Automatica e Informatica, Politecnico di Torino, Corso Duca degli Abruzzi 24, I-10129 Torino (Italy), e-mail Paolo.Prinetto@polito.it years, several methods [SSAb92] [RPGN94] [PRSR94] have been proposed, which combine this approach with the use of Genetic Algorithms (GAs) [Gold89]. Results demonstrated that the approach is very flexible and provides good results for large circuits, where other methods fail.However, the analysis we performed on the behavior of GATTO (the tool described in [PRSR94]) shows that the algorithm has some weakness points:• the cross-over operator is not as effective as in other problems GAs have been applied to;• the method can hardly determine the length of the sequences; this results in an increase of thetime required by the A TPG process, and of thenumber of generated vectors;• the phase devoted to find sequences which excite faults is purely random; this obviously decreasesthe method effectiveness in terms of achievedfault coverage and required CPU time.In this paper we introduce some new techniques to overcome the above problems. We devised a more ef-fective cross-over operator, and added new techniques which provide the method with the capability of auto-matically determining the minimal length of the test sequences. Finally, we re-arranged the whole algorithm in order to increase the effectiveness of the fault exci-tation phase, which is no longer purely random, but exploits information from the already generated se-quences. To experimentally prove the effectiveness of the proposed techniques we implemented an improved version of GATTO, named GATTO+. The results show substantial improvements in GATTO+: from one side, they are now comparable to the competing algorithms on the small- and medium-size circuits; from the other side, results are further enhanced on the largest ones.The paper is organized as follow: in Section 2 we briefly summarize the GATTO algorithm; Section 3 describes the improvements introduced in GATTO+. Section 4 presents the experimental results we gathered and provides some comparisons with other A TPG al-gorithms. Section 5 draws some conclusions.2. The GATTO algorithmThe GATTO algorithm, presented in [PRSR94], is organized in three phases:• the first phase aims at selecting one fault (denoted as target fault); this phase consists ofrandomly generating sequences and fault simu-lating them w.r.t. the untested faults. As soon asone sequence is able to excite at least one fault,the fault is chosen as target fault;• the second phase aims at generating a test se-quence for the target fault; it is implemented as aGenetic Algorithm: each individual is a test se-quence to be applied starting from the reset state;cross-over and mutation operators are defined tomodify the population and generate new indi-viduals; a fitness function evaluates how closeeach individual is to the final goal (i.e., detectingthe target fault); this function is a weighted sumof the numbers of gates and Flip-Flops having adifferent value in the good and faulty circuit.After a maximum number of unsuccessful gen-erations the target fault is aborted and the secondphase is exited;• the third phase is a fault simulation experiment which determines whether the test sequencepossibly generated in phase 2 detects other faults(fault dropping).The three phases are repeated till either all the faults have been tested or aborted, or a maximum number of iterations has been reached.3. ImprovementsBased on the results reported in [PRSR94], we real-ized that several points in the GATTO algorithm were still worth of improvements. We will describe them in details in the following subsections, together with the improved solutions we devised for each of them.3.1. Cross-Over OperatorThe cross-over operator adopted in GATTO belongs to the category denoted as two-cuts cross-over. The operator works in a horizontal manner: the new se-quence is composed of some vectors coming from ei-ther parents (Fig. 1), according to the position of two randomly generated cut points. Unfortunately, there is no guarantee that the vectors coming from the second parent produce in the new sequence the same behavior they produce in the parent sequence, as the state from which they are applied is different. As a consequence, we observed in GATTO that the off-spring of two good individuals was often a bad individual; in general, the cross-over operator was not as effective for the A TPG problem as it usually is for other problems GAs have been applied to.The cross-over operator defined for GATTO+ works in a vertical manner; the off-spring does not inherit whole vectors from parents: rather, the values for each input are taken either from one parent or from the other, depending on a random choice (Fig. 2), as the operator belongs to the category known as uniform cross-over. The length of the new sequence is that of the longest between the two parent sequences: inputs taken from the shortest parent are completed with ran-dom values (dark in Fig. 2).3.2. Test LengthIn GATTO it is up to the user to decide the initial test length, which is then automatically increased dur-ing the A TPG process. For some circuits, this results in a test length higher than the minimum one, while for other circuits the process spends many iterations for reaching the length required to test some faults. Moreover, the computational complexity of the whole process mainly depends on the cost of fault simulation; therefore, any unnecessary increase in the length of the sequences results in a corresponding waste in the re-quired CPU time.To face this problem we improved the GATTO al-gorithm in two ways: we first modified the evaluation function on which the fitness function is based, and then introduced new mutation operators.3.2.1. New Evaluation FunctionThe evaluation function adopted in GATTO is based on the following expressionh(v j k,f i)= c1*b1(v j k,f i)+c2*b2(v j k,f i)(1) which provides a measure of how close the k-th in-put vector v j k of a sequence s j is to detect the fault f i. In (1), c1 and c2 are constants, while b1 and b2 are functions, whose value is proportional to the number of gates and Flip-Flops (respectively) having a different value in the good and faulty circuit for fault f i. Once the value of h(v j k,f i)is known for every vector in thesequence, the evaluation function H for the sequence s j is computed asH(s j ,f i )=max k (h(v j k ,f i )) ∀ v j k ∈ s j (2)In order to bias the evolution towards the shortest sequences, a modified version H *(s j ,f i ) of the evaluation function H has been introduced in GATTO+;the value of h(v j k ,f i ) for the k -th vector of the j -th sequence is weighted with a coefficient whose value decreases with k ; the new evaluation function corre-sponds to the maximum value of the weighted function:H *(s j ,f i )=max k (HANDICAP k ·h(v j k ,f i )) ∀ v j k ∈ s j (3)where the value of the parameter HANDICAP ranges3.2.2. New Mutation OperatorsIn GATTO, any change in the length of the se-quences during phase 2 stems from the cross-over op-erator: in fact, the length of any new sequence can randomly vary up to the sum of the lengths of the two parent sequences. The new cross-over operator pre-sented in the previous Section behaves in a completely different way, and generates sequences as long as the longest parent. This means that the length of the se-quences in a population can never be higher than that of the longest one in the previous generation. Unfortu-nately, there is thus no way to increase the sequence length.To overcome this problem, and to force the algo-rithm to better explore all the search space, we intro-duce two new mutation operators (MO+ and MO-),which are activated on an existing sequence with a given activation probability:• MO+ introduces a randomly generated vector in a random position within the existing sequence;thanks to this operator, longer sequences are generated and evaluated;• MO- removes a randomly selected vector from the existing sequence: if the vector is not essen-tial, the evaluation function of the sequence in-creases.3.3. Fault Excitation Fault excitation is one of the most critical problems when devising a GA-based A TPG. In fact, no way has been found, up to now, to evaluate how close a se-TPGs proposed in the literature resort to a 2 all the sequences belonging to the last population are stored, and then used in the following phase 1 instead of the randomly generated ones (as in GATTO).If one of these sequences is able to excite at least one fault, this is selected as target fault, and a new phase 2 is activated. Otherwise, random sequences are generated trying to excite faults, like in GATTO. The pseudo-code of phase 1 is reported in Fig. 3.4. Experimental Results We implemented a prototypical version of GATTO+containing all the techniques described above: the new cross-over operator substitutes the old one, and the operators MO+ and MO-, as well as the parameter HANDICAP, have been introduced. The values of all the parameters have been experimentally determined through a preliminary set of runs: the operators MO+and MO- are activated with probability 0.05 and 0.1,the parameter HANDICAP holds the value 0.98, andC1 and C2 have been assigned the values 1 and 10,respectively. Tab. 1 reports the results in terms of Fault Coverage (FC), CPU time and test length for the whole set of ISCAS’89 circuits. Experiments have been per-4.1. GATTO+ vs. GATTOTo demonstrate the effectiveness of the described techniques we report in Tab. 2 a comparison with the results of GATTO published in [PRSR94], where only the largest ISCAS’89 circuits were considered. GATTO+ is able to increase the fault coverage in 11 cases out of 12, and in 6 cases the increase is greater than 4%. On the other side, the CPU time is decreased in 9 cases out of 12. For all the circuits, we were able either to increase the Fault Coverage by more than 4%, or to decrease the CPU time. GATTO+ achieves this result mainly thanks to the more effective technique adopted for phase 1, whose cost is now greatly de-creased. Concerning the test length, the number of test vectors generated by GATTO+ is sometimes higher than those of GATTO, due to the new sequences added to detect other faults.Tab. 1: GATTO+ performance on ISCAS’89 circuits.4.2. GATTO+ vs. other algorithmsW report in the following the data published for two other A TPG algorithms and concerning the ISCAS’89 benchmark circuits. In Tab. 3 we consider HITEC, a topological algorithm described in [NiPa91], and the GA-based A TPG proposed in [RPGN94]. The two algorithms were selected, as they are representative of the two categories we denoted above as topological and simulation-based A TPG algorithms; we did not con-sider any A TPG belonging to the category of symbolic ones, as they are not able to deal with large circuits, which are normally the most critical problem in the real world.Two difficulties must be faced when performing such a comparison: the first one concerns the hardware platform, which is different for the three A TPGs (results for HITEC were gathered on a SPARCstation 1, those in [RPGN94] on a SPARCstation II, and those for GATTO+ on a DECstation 3000/500). The second difficulty comes from the fact that GATTO+ assumes that all the Flip-Flops in the circuits are resettable, and generates sequences starting from the all-0s state, while the two other algorithms do not make this assumption, and generate sequences starting from the all-Xs state.Tab. 2: comparison between GATTO and GATTO+.Taking into account the two points above, the results in Tab. 3 show that:• GATTO+ is able to reach higher Fault Coverage figures in all cases but 4 when HITEC is consid-ered; the figures of GATTO+ are always betterwhen the tool of [RPGN94] is analyzed;• the CPU times required by GATTO+ are lower than the ones required by HITEC for all the cir-cuits but S1196 and S1238; on the other side,GATTO+ is always faster than the method in[RPGN94]. For most circuits, we believe that thespeed-up ratios are greater than any reasonablefactor due to the different hardware platforms. 5. ConclusionsWe described some advanced techniques to improve the effectiveness of a GA-based A TPG like GATTO [PRSR94]. They fully exploit the powerfulness of Evo-lutionary Computation by removing some weakness points concerning the cross-over operator, the ability to determine the optimal sequence length, and the fault excitation phase.Experimental results demonstrate that the new techniques are able to significantly improve the per-formance of GATTO in terms of Fault Coverage and CPU times. We also compared them with the results of a state-of-the-art topological algorithm and with the ones of another GA-based ATPG algorithm.As a main contribution, this paper experimentally demonstrates that a carefully tuned GA-based A TPG algorithm is able to provide better results than any other approach: in fact, symbolic techniques, although faster on the small circuits, do not work with the large ones, while topological techniques, although able to identify untestable faults, are generally slower.6. References[ACAg88]V.D. Agrawal, K.-T. Cheng, P. Agrawal,“CONTEST: A Concurrent Test Generator forSequential Circuits,” Proc. 25th Design Auto-mation Conf., 1988, pp. 84-89[CHSo93]H. Cho, G.D. Hatchel, F. Somenzi, “Redundancy Identification/Removal and Test Generation forSequential Circuits Using Implicit State Enu-meration,” IEEE Trans. on CAD/ICAS, Vol.CAD-12, No. 7, pp. 935-945, July 1993[Gold89] D.E. Goldberg, “Genetic Algorithms in Search, Optimization, and Machine Learning,” Addison-Wesley, 1989[NiPa91]T. Niermann, J.H. Patel, “HITEC: A Test Gen-erator Package for Sequential Circuits,” Proc.European. Design Aut. Conf., 1991, pp. 214-218 [PRSR94]P. Prinetto, M. Rebaudengo, M. Sonza Reorda,“An Automatic Test Pattern Generator for LargeSequential Circuits based on Genetic Algo-rithms,” Proc. Int. Test Conf., 1994, pp. 240-249 [RPGN94] E.M. Rudnick, J.H. Patel, G.S. Greenstein, T.M.Niermann, “Sequential Circuit Test Generationin a Genetic Algorithm Framework,” Proc. De-sign Automation Conf., 1994, pp. 698-704 [SSAb92] D.G. Saab, Y.G. Saab, J. Abraham, “CRIS: A Test Cultivation Program for Sequential VLSICircuits,” Proc. Int. Conf. on Comp. Aided De-sign, 1992, pp. 216-219。

grafting to合成方法

grafting to合成方法

grafting to合成方法Grafting is a method of combining parts from two separate plants to create a new plant with desired qualities. This technique is commonly used in horticulture to produce new fruit tree varieties, improve disease resistance, and enhance overall plant vigor. 合成方法是将两个独立植物的部分结合起来,以创造出具有期望特性的新植物的方法。

这种技术通常用于园艺业,以生产新的果树品种,提高抗病能力,并增强整体植物的活力。

One of the primary reasons for grafting is to create new plant varieties. By combining the desirable traits of two plants, such as fruit size, flavor, or overall growth habit, horticulturalists can develop unique and valuable cultivars. 一种嫁接的主要原因之一是创造新的植物品种。

通过结合两种植物的理想特性,如果实大小、风味或整体生长习性,园艺学家可以开发独特而有价值的栽培品种。

In addition to creating new varieties, grafting is also used to improve disease resistance in plants. By grafting a susceptible plant onto a disease-resistant rootstock, horticulturalists can create plants that are more resistant to common diseases, ultimately reducing the need forchemical pesticides and promoting sustainable and environmentally friendly practices. 除了创造新品种外,嫁接还用于提高植物的抗病能力。

unit1 introduction to methodology

unit1 introduction to methodology
English Language Teaching Methodology
Contents:
nguage and language Learning 2.Classroom management 3.Lesson Planning 4.Teaching Approaches and Methods 5.Pronunciation Teaching 6.Grammar Teaching 7.Vocabulary Teaching 8.Listening Teaching 9.Speaking teaching 10.Reading teaching 11.Writing Teaching 12.Lesson Plan Presentation 13.New Curriculum Standard 14.Multimedia Assisted Language Teaching 15.Self-development
张正东:《外语教育学》。科学出版社,1999年。 张正东、李少伶:《英语教学论》。陕西师范大学出版社,2003年。 张正东、杜培俸:《外语立体化教学法的原理与模式》。科学出版社,1998年。 胡春洞:《英语教学法》。高等教育出版社,1990年。 王才仁:《英语教学交际论》。广西教育出版社,1996年。 胡春洞、戴忠信:《英语阅读论》。广西教育出版社,1998年。 高兰生、陈辉岳:《英语测试论》。广西教育出版社,1996年。 聂希庸、曹宝健:《中学英语教学》。光明日报出版社,1998年。 程晓堂:《任务型语言教学》。高等教育出版社,2004年。 田式国:《英语教学理论与实践》。高等教育出版社,2001年。 宋桂月、金莺:《英语课程标准教师读本》。华中师范大学出版社,2002年。 鲁子问:《中小学英语真实任务教学实践论》。外语教学与研究出版社,2003年。 梁祝、卢福波:《小学英语新课程课堂教学案例》。广东高等教育出版社,2003年。 程可拉、刘津开:《中学英语任务型教学理念与教学示例》。华南理工大学出版社,2005年。 张玲棣:《高中英语课堂教学设计与案例》。高等教育出版社,2004年。 于勇:《中小学课堂教学技能训练》。当代世界出版社,2001年 顾曰国:《英语教学法》(上下)。外语教学与研究出版社,1998年。 王蔷:《英语教学法教程》。高等教育出版社,2005年。 王蔷:《小学英语教学法教程》。高等教育出版社,2003年。 肖惜:《英语教师职业技能训练简明教程》。高等教育出版社。1999年。 罗少茜:《英语课堂教学形成性评价研究》。外语教学与研究出版社。2003年。 Carol MORGAN and Peter NEIL:Teaching Modern Foreign Languages.Kogan Page,2001年。 Adrian Doff: Teach English A training course for teachers TRAINER’S HANDBOOK Alan Pritchard: Ways of Learning. David Fulton,2005年。

广东2023英语高考题型

广东2023英语高考题型

广东2023英语高考题型广东2023英语高考题型 12023年普通高等学校招生全国统一考试(新课标I)英语试题注意事项:1.答卷前,考生务必将自己的姓名、准考证号填写在答题卡上。

2.回答选择题时,选出每小题答案后,用铅笔把答题卡上对应题目的答案标号涂黑。

如需改动,用橡皮擦干净后,再选涂其他答案标号。

回答非选择题时,将答案写在答题卡上,写在本试卷上无效。

3.考试结束后,将本试卷和答题卡一并交回。

第一部分听力(共两节,满分30分)做题时,先将答案标在试卷上。

录音内容结束后,你将有两分钟的时间将试卷上的答案转涂到答题卡上。

2023年高考英语新课标124:56第一节(共5小题;每小题1.5分,满分7.5分)听下面5段对话。

每段对话后有一个小题,从题中所给的A、B、C三个选项中选出最佳选项,并标在试卷的相应位置。

听完每段对话后,你都有10秒钟的时间来回答有关小题和阅读下一-小题。

每段对话仅读一遍。

例: How much is the shirt?A. £ 19.15.B. £ 9.18.C. £ 9.15.答案是C。

1. What will Jack probably do this weekend?A. Go camping.B. Visit a friend.C. Watch a film.2. What does the woman ask the man to do?A. Take care of her bags.B. Pack the food for her.C. Check the train schedule.3. When will the man see Bob?A. This Friday.B. This Saturday.C. Next Monday.4. Why does the man apologize?A. For the terrible food.B. For the overcharge.C. For the waiter's rudeness.5. What are the speakers talking about?A. Writing a book.B. Holding a celebration.C. Buying a present.第二节(共15小题;每小题1.5 分,满分22.5分)听下面5段对话或独白。

Theoretical approaches to heterogeneous catalysis

Theoretical approaches to heterogeneous catalysis

Theoretical approaches toheterogeneous catalysisHeterogeneous catalysis refers to chemical reactions where the reactants are in different phases, such as a gas and a solid. These reactions play a crucial role in the chemical and pharmaceutical industries, as they allow efficient production of complex molecules under mild conditions. Despite their practical importance, the underlying mechanism of heterogeneous catalysis is still not fully understood. In this article, we will discuss some of the theoretical approaches that have been developed to explain these reactions.One of the earliest models in heterogeneous catalysis is the Langmuir-Hinshelwood (LH) mechanism, which states that the reaction takes place on the surface of the catalyst through a series of adsorption and desorption steps. According to the LH model, the first step is the adsorption of the reactants onto the catalyst surface. This is followed by the formation of an activated complex, which has higher energy than both the reactants and the products. Finally, the complex dissociates to yield the products. The rate of the reaction is determined by the rate of the slowest step, which is usually the formation of the activated complex.The LH model provides a useful framework for understanding heterogeneous catalysis, but it has some limitations. For example, it does not account for the presence of spectator species, which can influence the reaction rate by blocking or enhancing certain sites on the catalyst surface. It also assumes that the catalyst surface is homogeneous and does not consider the effects of structural defects or dopants.To address these shortcomings, a number of more advanced models have been developed. One such model is the Mars-van Krevelen (MvK) mechanism, which takes into account the role of oxygen in redox reactions. The MvK model proposes that the reaction occurs through a series of interactions between the catalyst surface and oxygenmolecules. These interactions can lead to the formation of oxygen vacancies, which can then react with the adsorbed reactants to form the desired products.Another important model in heterogeneous catalysis is the Sabatier principle. The Sabatier principle states that the optimal catalyst for a given reaction is one that has a binding energy that is just strong enough to hold the reactants in place, but not so strong that it prevents their reaction. This principle has been used to design new catalysts for a variety of reactions, including the hydrogenation of carbon dioxide to produce methanol and the selective oxidation of methane to methanol.More recently, density functional theory (DFT) has emerged as a powerful tool for understanding heterogeneous catalysis at the molecular level. DFT allows researchers to calculate the electronic and geometric properties of catalyst surfaces and predict their reactivity towards different reactants. DFT has been used to study a wide range of reactions, from the activation of small molecules such as hydrogen and oxygen to the selective oxidation of hydrocarbons.In conclusion, while there is still much to learn about heterogeneous catalysis, the toolkit of theoretical approaches available to researchers is constantly expanding. From the early models of LH and MvK to the more recent use of DFT, each approach provides a unique perspective on the mechanisms that govern heterogeneous catalytic reactions. By combining these approaches, researchers can gain a deeper understanding of the complex and multifaceted nature of catalytic processes and design new catalysts with improved activity, selectivity, and stability.。

乳铁蛋白生物活性肽及其功能机制研究进展

乳铁蛋白生物活性肽及其功能机制研究进展

乳铁蛋白生物活性肽及其功能机制研究进展石璞洁1,许诗琦2,王震宇2,吴 超2,卢卫红1,*,杜 明1,2,*(1.哈尔滨工业大学化工与化学学院,黑龙江 哈尔滨 150001;2.大连工业大学食品学院,海洋食品深加工省部共建协同创新中心,国家海洋食品工程技术研究中心,辽宁 大连 116034)摘 要:乳铁蛋白是一种天然铁结合糖蛋白,广泛分布于哺乳动物的乳清和大部分生物体液中。

人体摄入的乳铁蛋白经蛋白酶水解后,主要以肽的形式被消化吸收,从而发挥其生理功能。

近年来,国内外研究报道了多种具有广谱抗菌、抗肿瘤、降血压、抗炎和免疫调节功能的乳铁蛋白生物活性肽,它们具有调节机体生理功能和为机体提供营养的双重功效,因此对人体健康有重要作用。

本文主要对乳铁蛋白生物活性肽的种类、结构及其作用机制进行了综述,并探讨了其在食品领域中的应用,以期为乳铁蛋白来源的生物活性肽的研究和相关功能食品的开发提供理论参考。

关键词:乳铁蛋白;生物活性肽;抗菌;抗肿瘤;降血压Advances in Biological Activity and Functional Mechanism of Peptides from LactoferrinSHI Pujie 1, XU Shiqi 2, WANG Zhenyu 2, WU Chao 2, LU Weihong 1,*, DU Ming 1,2,*(1. School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China; 2. Collaborative Innovation Center of Provincial and Ministerial Co-construction for Seafood Deep Processing, National Engineering Research Center ofSeafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China)Abstract: Lactoferrin is an iron-binding natural glycoprotein, which is distributed widely in the milk of various mammalian species and most biological fluids. Ingested lactoferrin is mainly adsorbed as peptides by the human body after protease digestion to exert its physiological functions. In recent years, a great number of bioactive peptides with antimicrobial, antitumor, antihypertension, anti-inflammatory and immune modulator activities derived from lactoferrin hydrolysates have been reported. These peptides have dual functions of regulating the body ’s physiological functions and providing nutrients for the body, thereby playing an important role in human health. In this review, the species, structures and mechanisms of action of functional peptides from lactoferrin are summarized and their applications in the field of food science are discussed to provide a theoretical basis for the research of these bioactive peptides and their applications in the functional food field.Keywords: lactoferrin; bioactive peptides; antibacterial; anticancer; antihypertensive DOI:10.7506/spkx1002-6630-20200429-379中图分类号:TS252.1 文献标志码:A 文章编号:1002-6630(2021)07-0267-08引文格式:石璞洁, 许诗琦, 王震宇, 等. 乳铁蛋白生物活性肽及其功能机制研究进展[J]. 食品科学, 2021, 42(7): 267-274. DOI:10.7506/spkx1002-6630-20200429-379. SHI Pujie, XU Shiqi, WANG Zhenyu, et al. Advances in biological activity and functional mechanism of peptides from lactoferrin[J]. Food Science, 2021, 42(7): 267-274. (in Chinese with English abstract) DOI:10.7506/spkx1002-6630-20200429-379. 收稿日期:2020-04-29基金项目:国家自然科学基金面上项目(31771926)第一作者简介:石璞洁(1992—)(ORCID: 0000-0002-2658-9373),女,博士研究生,研究方向为蛋白质科学。

RANKING OF GRAPH PATTERNS

RANKING OF GRAPH PATTERNS

专利名称:RANKING OF GRAPH PATTERNS发明人:TUCKER, Robert,KERR, Robert,NATHAN, Martin,MARTINDALE, Dominique申请号:IB2019/056139申请日:20190718公开号:WO2020/021401A1公开日:20200130专利内容由知识产权出版社提供专利附图:摘要:A query comprising a graph pattern comprising a plurality of graph triples of node-edge-node is received. A graph database comprising a plurality of graph patterns is accessed and a plurality of graph patterns in the graph database that match the receivedquery are identified. An inverse frequency for each graph triple of the received query in the accessed graph database is calculated and from this a score for each graph pattern in the graph database that matches the received query is also calculated, the score comprising a sum of the inverse frequencies for each graph triple contained within the respective graph pattern. The plurality of graph patterns in the graph database that match the received query are then ranked according to their respective calculated scores.申请人:INTERNATIONAL BUSINESS MACHINES CORPORATION,IBM UNITED KINGDOM LIMITED,IBM (CHINA) INVESTMENT COMPANY LIMITED地址:New Orchard Road Armonk, NY 10504 US,PO Box 41, North Harbour Portsmouth, Hampshire PO6 3AU GB,25/F, Pangu Plaza No.27, Central North 4th Ring Road Chaoyang District Beijing 100101 CN国籍:US,GB,CN代理人:GRAHAM, Timothy更多信息请下载全文后查看。

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Table 1: The integer notation of pitch classes. Although plenty of research works of post-tonal music analysis have been done by musicologists, most of them focus on analyzing and comparing a limited number of music compositions. Comparative study across numerous compositions is difficult because the identification of pitch-class sets occurred in compositions—an important aspect of pitchclass set theory—is highly time-consuming and error-prone when performed manually. Large-scale comparative study requires an effective post-tonal music analysis system. There are software utilities to assist post-tonal music analysis [21, 16, 18], but none of them considers searching for pitch-class sets. [7, 9, 6] investigated how to choose groups of musical notes for analysis. Peter Castine published his Ph.D thesis on computer-aided analysis on post-tonal music [4]. However, he focused on how graphical user interface could help in pitch-class set theory analysis. A Pascal routine named Setsearch is presented in the book Pascal Programming for Music Research [3]. It locates pitch-class sets by using a sequential searching algorithm with a sliding frame. However, it is difficult to determine the frame size as the author mentioned. Improper value of the frame size may cause false dismissals, i.e. some matched patterns are missed. The fundamental problem of pattern matching in post-tonal music analysis is on the definition of matching condition(s). In Figure 1, given the pitch-class set query {7, 8, 11}, the first matched pattern is the first three notes of the melody but not including the minim 2
Abstract A graph-theoretical approach is proposed to facilitate pattern matching in posttonal music analysis with pitch-class set theory, in which music perception is taken into account by incorporating stream segregation—a model of the perceptual organization of music. A piece of music is modelled as a graph, with each musical note presented as a vertex and the relationship between a pair of musical notes as an edge. The relationship is determined by stream segregation. According the proposed matching conditions, searching for a musical pattern is equivalent to searching for a special subgraph called maximal matched CRP subgraph. The comparisons are made between the patterns identified by the graph-theoretical approach and those by the musicologists.
1
Introduction
One of the most significant areas in music research, analysis of post-tonal music—art music that began in Europe in the early 20th century, significant composers of which include Bela Bartok, Alban Berg, Arnold Schoenberg, Igor Stravinsky, Anton Webern— consists of studying how this music works by finding the building blocks as unifying cells in such a musical work. Searching for these building blocks or musical patterns can be modelled as a pattern matching problem: given a musical pattern query and a music database, the aim is to search for all matched patterns in the database. In this paper, the pattern matching problem in post-tonal music analysis is based on pitch-class set theory —the widest currency seen as the core system for post-tonal music analysis [1]. The idea of pitch-class set theory is illustrated in the following example in [14]. The opening of Arnold Schoenberg’s Klavierst¨ ucke, Op. 11, No. 1 is shown in Figure 1. Although the first three notes of the melody and the sustained notes in bars 4-5 look quite dissimilar, these two collections of notes are, in the perspective of pitch-class set theory, equivalent building blocks because they both contain a B, a G , and a G . Twentieth-century composers have greater freedom in exploring ways to unify a music composition and pitch-class set theory aims to uncover building blocks hidden in the music. A building block is called a pitch-class set, denoting a group of pitch classes. A 1
Mäßige q

Piano

p








Figure 1: The opening of Arnold Schoenberg’s Klavierst¨ ucke, Op. 11, No. 1.
Integer Pitch-class name content 0 B , C, D 1 C,D 2 C∗, D, E 3 D,E 4 D∗, E, F 5 E , F, G
Integer Pitch-class name content 6 F,G 7 F∗, G, A 8 G,A 9 G∗, A, B 10 A,B 11 A∗, B, C
A Graph-Theoretical Approach for Pattern Matching in Post-Tonal Music Analysis
Wai Man Szeto and Man Hon Wong Department of Computer Science and Engineering The Chinese University of Hong Kong Shatin, N.T., Hong Kong {wmszeto, mhwong}@.hk
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