Correction to “Tensor Products of Modules and the Rigidity of Tor”,
ICH Q1D 新原料药和制剂稳定性试验的括号法和矩阵法设计 中英文对照版 2020.4.29
外征求意见。
10 November
2000 2000 年 11 月 10 日
New Codification November
2005 新编码
2005 年 11 月 Q1D
Q1D
Current Step 4 version 当前第四阶段版本
Approval by the Steering Committee under Step 4 and recommendation for adoption to the three ICH regulatory bodies. 经指导委员会批准作为第四阶段版本推荐给
1.3 Scope of the Guideline 范围 This document provides guidance on bracketing and matrixing study designs. Specific principles are defined in this guideline for situations in which bracketing or matrixing can be applied. Sample designs are provided for illustrative purposes, and should not be considered the only, or the most appropriate, designs in all cases. 本文件为括号法和矩阵法的研究设计提供指导,并对可以应用括号法和矩阵法的情形制订了 一些特殊的原则。本文提供的举例设计仅作演示,不应认为它是所有情况下的唯一或最适宜 的设计方法。
关于海洋生态系统中的营养循环 英语作文
Nutrient Cycling in Marine EcosystemsNutrient cycling is a fundamental process in marine ecosystems,playing a crucial role in maintaining the health and productivity of oceanic environments.The cycling of essential nutrients such as nitrogen, phosphorus,and carbon supports the growth of marine organisms, drives primary production,and sustains the complex food webs that characterize marine ecosystems.This essay explores the mechanisms, significance,and challenges of nutrient cycling in marine environments.Mechanisms of Nutrient CyclingBiological ProcessesPhotosynthesis and RespirationPhotosynthesis and respiration are key biological processes that drive nutrient cycling in marine ecosystems.Phytoplankton,the primary producers in the ocean,use sunlight to convert carbon dioxide(CO2) and inorganic nutrients into organic matter through photosynthesis.This process not only produces oxygen but also forms the base of the marine food web.When phytoplankton and other marine organisms respire, they release CO2and other nutrients back into the water,making them available for reuse.DecompositionDecomposition is another critical biological process in nutrient cycling. When marine organisms die,their bodies are broken down by bacteria and other decomposers.This decomposition process releases nutrients such as nitrogen and phosphorus back into the water column,where they can be taken up by primary producers.The recycling of nutrients through decomposition ensures the continuous availability of essential elements for marine life.Physical ProcessesUpwellingUpwelling is a physical process that brings nutrient-rich deep water to the surface.This occurs when wind-driven currents push surface water away from the coast,allowing deeper water to rise and replace it. Upwelling zones are among the most productive areas in the ocean, supporting high levels of primary production and rich biodiversity.The influx of nutrients from upwelling fuels the growth of phytoplankton, which in turn supports higher trophic levels.Ocean CurrentsOcean currents play a significant role in the distribution and cycling of nutrients.These currents transport nutrients across vast distances, connecting different regions of the ocean.For example,the thermohaline circulation,also known as the global conveyor belt,moves nutrient-rich water from the deep ocean to the surface and from the poles to the equator.This circulation helps maintain nutrient balance and supports marine productivity on a global scale.Chemical ProcessesNitrogen FixationNitrogen fixation is a chemical process that converts atmospheric nitrogen(N2)into a form that can be used by marine organisms,such as ammonium(NH4+).This process is carried out by specialized bacteria and cyanobacteria,known as diazotrophs.Nitrogen fixation is essential for replenishing the nitrogen supply in marine ecosystems,as nitrogen is a critical nutrient for the growth of phytoplankton and other organisms.DenitrificationDenitrification is the process by which nitrate(NO3-)is converted back into nitrogen gas(N2),which is then released into the atmosphere.This process is carried out by anaerobic bacteria in low-oxygen environments, such as sediments and oxygen minimum zones.Denitrification helps regulate the nitrogen cycle by removing excess nitrogen from the ocean, preventing nutrient over-enrichment and maintaining ecological balance.Significance of Nutrient CyclingSupporting Primary ProductionNutrient cycling is essential for supporting primary production in marine ecosystems.The availability of nutrients such as nitrogen,phosphorus, and iron determines the growth and productivity of phytoplankton. These primary producers form the base of the marine food web, providing energy and nutrients for higher trophic levels,including zooplankton,fish,and marine mammals.Healthy nutrient cycling ensures a continuous supply of essential elements,sustaining the productivity and biodiversity of marine ecosystems.Maintaining Ecosystem BalanceNutrient cycling helps maintain the balance and stability of marine ecosystems.The recycling of nutrients through biological,physical,and chemical processes ensures that essential elements are available in the right amounts and forms for marine organisms.This balance prevents nutrient deficiencies and excesses,which can lead to ecological disruptions such as algal blooms,hypoxia,and loss of biodiversity. Effective nutrient cycling supports the resilience and health of marine ecosystems.Carbon SequestrationNutrient cycling plays a role in carbon sequestration,a process that removes CO2from the atmosphere and stores it in the ocean. Phytoplankton photosynthesis captures CO2,and a portion of the organic carbon produced is transported to the deep ocean through the biological pump.This sequestration of carbon in the deep ocean helps mitigate the impact of greenhouse gas emissions and contributes to climate regulation.Nutrient cycling is thus interconnected with the global carbon cycle and climate system.Challenges in Nutrient CyclingHuman ImpactsHuman activities have significantly altered nutrient cycling in marine ecosystems.Agricultural runoff,wastewater discharge,and industrial pollution introduce excessive nutrients,particularly nitrogen andphosphorus,into coastal waters.This nutrient over-enrichment,known as eutrophication,can lead to harmful algal blooms,hypoxia(low oxygen levels),and the degradation of marine habitats.Managing nutrient inputs and reducing pollution are critical for protecting nutrient cycling processes.Climate ChangeClimate change poses a major challenge to nutrient cycling in marine ecosystems.Rising sea temperatures,ocean acidification,and changes in ocean circulation patterns can disrupt the distribution and availability of nutrients.For example,warming waters can reduce the efficiency of upwelling,limiting the supply of nutrients to surface waters.Ocean acidification can affect the ability of certain organisms,such as shell-forming plankton,to utilize nutrients effectively.Understanding and mitigating the impacts of climate change on nutrient cycling is essential for sustaining marine ecosystems.OverfishingOverfishing can disrupt nutrient cycling by altering the structure and function of marine food webs.The removal of key species,such as top predators and herbivores,can have cascading effects on nutrient dynamics.For example,the decline of large predatory fish can lead to an increase in smaller fish and invertebrates,which may alter nutrient recycling rates and patterns.Sustainable fishing practices are necessary to maintain the integrity of nutrient cycling processes.ConclusionNutrient cycling is a vital process that sustains the health and productivity of marine ecosystems.Through biological,physical,and chemical mechanisms,essential nutrients are recycled and made available to marine organisms,supporting primary production, ecosystem balance,and carbon sequestration.However,human impacts, climate change,and overfishing pose significant challenges to nutrient cycling.Addressing these challenges requires effective management and conservation strategies to protect nutrient cycling processes and ensure the resilience and sustainability of marine ecosystems.By understandingand valuing the importance of nutrient cycling,we can work towards a healthier and more productive ocean.。
化妆品清洗和消毒培训
INCIDENT LEARNING - C&D
Water/Dilute Product Residues Left in Plant
Undrainable pipe arrangement Undrainable pump arrangement
Ensure water/product drains from all areas of plant by good design practice. If non drainable areas are identified, they MUST be managed by disinfection procedures prior to use of the plant
It may take in excess of 15 minutes for all parts of plant circuit (varies with configuration/equipment) to reach this temperature. Validation time/temperature is CRITICAL
Why Clean
Process
Purpose to remove product soils and residues to produce clean equipment surfaces that will not taint the subsequent production of a differing variant.
Recognised that greater emphasis on adherence to best practice principles is required. On site validation of procedures is a key area that needs to be improved.
转基因食品英文ppt课件
ONE
KEEP VIEW
English PowerPoint courseware for genetically modified foods
REPORTING
CATALOGUE
目 录
• Introduction to Genetically Mo • Benefits of Genetically Modifi • Risks of Genetically Modified • Labeling of Genetically Modified • Non GMO Alternatives
Protection against harmful effects
Some GM foods may have harmful effects on human health Labeling allows consumers to avoid these foods if they choose to
Global labeling standards
Codex Alimentarius
The Codex Alimentarius is a set of international food standards and guidelines developed by the Food and Agriculture Organization of the United Nations and the World Health Organization It includes labeling standards for GM foods
PART 01
Introduction to Genetically Mo
What are Genetically Modified Foods?
化学化工英语试题及答案
化学化工英语试题及答案一、选择题(每题2分,共20分)1. Which of the following is a chemical element?A. WaterB. OxygenC. HydrogenD. Carbon答案:B, C, D2. The chemical formula for table salt is:A. NaOHB. NaClC. HClD. NaHCO3答案:B3. What is the process called when a substance changes from a solid to a liquid?A. SublimationB. VaporizationC. MeltingD. Condensation答案:C4. In the periodic table, which group contains alkali metals?A. Group 1B. Group 2C. Group 17D. Group 18答案:A5. What is the name of the process where a substance decomposes into two or more substances due to heat?A. CombustionB. OxidationC. ReductionD. Decomposition答案:D6. Which of the following is a physical property of a substance?A. ColorB. TasteC. SolubilityD. Reactivity答案:A7. What is the term for a compound that releases hydrogen ions (H+) when dissolved in water?A. BaseB. AcidC. SaltD. Neutral答案:B8. The law of conservation of mass states that in a chemical reaction:A. Mass is lostB. Mass is gainedC. Mass remains constantD. Mass can be converted into energy答案:C9. Which of the following is a type of chemical bond?A. Ionic bondB. Covalent bondC. Hydrogen bondD. All of the above答案:D10. What is the name of the process where a substance absorbs energy and changes from a liquid to a gas?A. MeltingB. VaporizationC. SublimationD. Condensation答案:B二、填空题(每题2分,共20分)1. The symbol for the element iron is ________.答案:Fe2. The pH scale ranges from ________ to ________.答案:0 to 143. A compound that produces a basic solution when dissolvedin water is called a ________.答案:base4. The smallest particle of an element that retains its chemical properties is called a ________.答案:atom5. The process of separating a mixture into its individual components is known as ________.答案:separation6. The study of the composition, structure, and properties of matter is called ________.答案:chemistry7. The process of a substance changing from a gas to a liquid is called ________.答案:condensation8. A(n) ________ reaction is a type of chemical reactionwhere two or more substances combine to form a single product. 答案:synthesis9. The volume of a gas at constant temperature and pressureis directly proportional to the number of ________.答案:moles10. The process of converting a solid directly into a gas without passing through the liquid phase is known as ________. 答案:sublimation三、简答题(每题10分,共30分)1. Explain what is meant by the term "stoichiometry" in chemistry.答案:Stoichiometry is the calculation of the relative quantities of reactants and products in a chemical reaction.It is based on the law of conservation of mass and involvesthe use of balanced chemical equations and the molar massesof substances to determine the amounts of reactants needed to produce a certain amount of product or the amounts ofproducts formed from a given amount of reactant.2. Describe the difference between a physical change and a chemical change.答案:A physical change is a change in the state or form of a substance without altering its chemical composition. Examples include melting, freezing, and boiling. A chemical change, on the other hand, involves a change in the chemical composition of a substance, resulting in the formation of new substances. Examples include combustion and rusting.3. What are the three main types of chemical bonds, and givean example of each.答案:The three main types of chemical bonds are ionic bonds, covalent bonds, and metallic bonds. An ionic bond is formed when electrons are transferred from one atom to another, resulting in the formation of oppositely charged ions. An example is the bond between sodium (Na) and chloride (Cl) in table salt (NaCl). A covalent bond is formed when two atoms share electrons, as seen in water (H2O) where hydrogen atoms share electrons with oxygen. Metallic bonds occur in metals, where a "sea" of delocalized electrons is shared among positively charged metal ions, as in sodium metal。
2021年-医学院硕士研究生入学考试试卷-模拟考_生物化学532
5
18、 属 于 真 核 生 物 染 色 质 中DNA的 三 级 结 构 的 描 述 是 A: 核 苷 酸 在 核 酸 长 链 上 的 排 列 顺 序 B : tRNA的 三 叶 草 结 构 C:DNA双 螺 旋 结 构 D:DNA的 核 小 体 结 构 19、 关于微粒体氧化体系的叙 述, 下列哪项是错误 的? A: 伴 有 磷 酸 化 B : 不 产 生ATP C: 可 参 与 体 内 代 谢 物 的 生 物 转 化 D: 可 参 与 药 物 的 生 物 转 化 20、 关于蛋白质变性, 下列说法哪项错 误? A: 其 非 共 价 键 破 坏 B: 其黏度降低 C: 其 四 级 结 构 破 坏 D: 其 二 硫 键 破 坏 21、 磷酸化酶的变构抑制剂是 A : ATP B :cAMP C :AMP D: 乙 酰CoA 22、 蛋白质紫外吸收的最大波长是 A: 2 7 0nm B: 2 5 0nm
12
C: 酶 的 活 性 中 心 未 形 成 或 未 暴 露 D: 酶 原 已 经 变 性 51、 肾中产生的氨主要来自 A: 氨 基 酸 联 合 脱 氨 基 作 用 B: 谷氨酰胺的水解 C: 嘌 呤 核 苷 酸 循 环 D: 尿 素 的 水 解 52、 分 析 组 织 细 胞 中 的 某 种mRNA水 平 时 , 采 用 哪 种 方 法 最 合 适 A: Southernblot B: Northernblot C: Westernblot D: Dotblot 53、 下列有关核酸分子杂交的叙述, 不正确的是 A: 杂 交 可 发 生 在 碱 基 序 列 完 全 互 补 的 核 酸 分 子 之 间 B: 杂交可发生在碱基序列部分互补的核酸分子之间 C: 具 有 双 螺 旋 结 构 的 核 酸 分 子 之 间 才 能 杂 交 D:RNA与DNA分 子 之 间 可 以 杂 交 54、 竞争性抑制剂作用是 A: 较 牢 固 地 与 酶 分 子 上 一 类 或 几 类 必 需 基 团 结 合 B: 占据酶活性中心阻止作用物与酶结合 C: 酶 可 以 与 作 用 物、 抑 制 剂 同 时 结 合 D: 抑 制 剂 能 与 酶 一 作 用 物 复 合 物 [ES] 结 合 , 不 能 与 游 离 酶 结 合 55、 原癌基因 sis 家族的表达产物是
Fluoride in drinking water and its removal
Journal of Hazardous Materials B137(2006)456–463Fluoride in drinking water and its removalMeenakshi∗,R.C.MaheshwariCentre for Rural Development and Technology,Indian Institute of Technology,Delhi,Hauz Khas,New Delhi,IndiaReceived2March2005;received in revised form15February2006;accepted16February2006Available online28February2006AbstractExcessivefluoride concentrations have been reported in groundwaters of more than20developed and developing countries including India where19states are facing acutefluorosis problems.Various technologies are being used to removefluoride from water but still the problem has not been rooted out.In this paper,a broad overview of the available technologies forfluoride removal and advantages and limitations of each one have been presented based on literature survey and the experiments conducted in the laboratory with several processes.It has been concluded that the selection of treatment process should be site specific as per local needs and prevailing conditions as each technology has some limitations and no one process can serve the purpose in diverse conditions.©2006Published by Elsevier B.V.Keywords:Fluoride;Fluorosis;Ground water;Soil water;Drinking water;Treatment1.IntroductionWater is an essential natural resource for sustaining life and environment that we have always thought to be available in abundance and free gift of nature.However,chemical compo-sition of surface or subsurface is one of the prime factors on which the suitability of water for domestic,industrial or agri-cultural purpose depends.Freshwater occurs as surface water and groundwater.Though groundwater contributes only0.6% of the total water resources on earth,it is the major and the pre-ferred source of drinking water in rural as well as urban areas, particularly in the developing countries like India because treat-ment of the same,including disinfection is often not required. It caters to80%of the total drinking water requirement and 50%of the agricultural requirement in rural India.But in the era of economical growth,groundwater is getting polluted due to urbanization and industrialization.Over the past few decades,the ever-growing population, urbanization,industrialization and unskilled utilization of water resources have led to degradation of water quality and reduction in per capita availability in various developing countries.Due to various ecological factors either natural or anthropogenic, the groundwater is getting polluted because of deep percolation ∗Corresponding author.E-mail address:mpahwa2000@(Meenakshi).from intensively cultivatedfields,disposal of hazardous wastes, liquid and solid wastes from industries,sewage disposal,sur-face impoundments etc.[1–4].During its complexflow history, groundwater passes through various geological formations lead-ing to consequent contamination in shallow aquifers.Presence of various hazardous contaminants likefluoride, arsenic,nitrate,sulfate,pesticides,other heavy metals etc.in underground water has been reported from different parts of India[5–9].In many cases,the water sources have been ren-dered unsafe not only for human consumption but also for other activities such as irrigation and industrial needs.Therefore,now there is a need to focus greater attention on the future impact of water resources planning and development taking into con-sideration all the related issues.In India,fluoride is the major inorganic pollutant of natural origin found in groundwater.In this paper detailed review on sources,ill effects and techniques available forfluoride removal is done.2.Occurrence and sourcesFluoride in minute quantity is an essential component for normal mineralization of bones and formation of dental enamel [10].However,its excessive intake may result in slow,progres-sive crippling scourge known asfluorosis.There are more than 20developed and developing nations that are endemic forflu-orosis.These are Argentina,U.S.A.,Morocco,Algeria,Libya,0304-3894/$–see front matter©2006Published by Elsevier B.V. doi:10.1016/j.jhazmat.2006.02.024Meenakshi,R.C.Maheshwari/Journal of Hazardous Materials B137(2006)456–463457 Table1Districts known to be endemic forfluoride in various states of India[18]States Districts Range offluorideconcentration(mg/L) Assam Karbianglong,Nagaon0.2–18.1Andhra Pradesh All districts except Adilabad,Nizamabad,West Godhavari,Visakhapattnam,Vijzianagaram,Srikakulam0.11–20.0Bihar Palamu,Daltonganj,Gridh,Gaya,Rohtas,Gopalganj,Paschim,Champaran0.6–8.0Delhi Kanjhwala,Najafgarh,Alipur0.4–10.0Gujarat All districts except Dang 1.58–31.0Haryana Rewari,Faridabad,Karnal,Sonipat,Jind,Gurgaon,Mohindergarh,Rohtak,Kurukshetra,Kaithal,Bhiwani,Sirsa,Hisar0.17–24.7Jammu and Kashmir Doda0.05–4.21 Karnataka Dharwad,Gadag,Bellary,Belgam,Raichur,Bijapur,Gulbarga,Chitradurga,Tumkur,Chikmagalur,Manya,Banglore,Mysore0.2–18.0Kerala Palghat,Allepy,Vamanapuram,Alappuzha0.2–2.5 Maharashtra Chandrapur,Bhandara,Nagpur,Jalgaon,Bulduna,Amravati,Akola,Yavatmal,Nanded,Sholapur0.11–10.2Madhya Pradesh Shivpuri,Jabua,Mandla,Dindori,Chhindwara,Dhar,Vidhisha,Seoni,Sehore,Raisen and Bhopal0.08–4.2Orrissa Phulbani,Koraput,Dhenkanal0.6–5.7Punjab Mansa,Faridcot,Bhatinda,Muktsar,Moga,Sangrur,Ferozpur,Ludhiana,Amritsar,Patila,Ropar,Jallandhar,Fatehgarh sahib0.44–6.0 Rajasthan All the32districts0.2–37.0 Tamilnadu Salem,Periyar,Dharampuri,Coimbatore,Tiruchirapalli,Vellore,Madurai,Virudunagar 1.5–5.0Uttar Pradesh Unnao,Agra,Meerut,Mathura,Aligarh,Raibareli,Allahabad0.12–8.9West Bengal Birbhum,Bhardaman,Bankura 1.5–13.0Egypt,Jordan,Turkey,Iran,Iraq,Kenya,Tanzania,S.Africa, China,Australia,New Zealand,Japan,Thailand,Canada,Saudi Arabia,Persian Gulf,Sri Lanka,Syria,India,etc.[11].In India, it wasfirst detected in Nellore district of Andhra Pradesh in1937 [12].Since then considerable work has been done in different parts of India to explore thefluoride laden water sources and their impacts on human as well on animals[13–17].At present, it has been estimated thatfluorosis is prevalent in17states of India(Table1).The safe limit offluoride in drinking water is1.0mg/L[19]. The endemicfluorosis in India is largely of hydrogeochemical origin.It has been observed that low calcium and high bicar-bonate alkalinity favor highfluoride content in groundwater [20,21].Water with highfluoride content is generally soft,has high pH and contains large amount of silica.In groundwater, the natural concentration offluoride depends on the geolog-ical,chemical and physical characteristics of the aquifer,the porosity and acidity of the soil and rocks,temperature,the action of other chemicals and the depth of wells.Due to large number of variables,thefluoride concentrations in groundwa-ter range from well under1.0mg/L to more than35.0mg/L [22].As the amount of water consumed and consequently the amount offluoride ingested is influenced primarily by air tem-perature,USPHS[23]has set a range of concentrations for maximum allowablefluoride in drinking water for communi-ties based on the climatic conditions as shown in Table2.Fluorine is highly reactive and is found naturally as CaF2. It is an essential constituent in minerals like topaz,fluorite,fluorapatite,cryolite,phosphorite,theorapatite,etc.[24].The fluoride is found in the atmosphere,soil and water.It enters the soil through weathering of rocks,precipitation or waste run off. Surface waters generally do not contain more than0.3mg/L of fluoride unless they are polluted from external sources.Though drinking water is the major contributor(75–90%of daily intake), other sources offluoride poisoning are food,industrial exposure, drugs,cosmetics,etc.[25].Thefluoride content of some major food products is given in Table3.3.Health impacts offluorideFluorine being a highly electronegative element has extraor-dinary tendency to get attracted by positively charged ions like calcium.Hence the effect offluoride on mineralized tissues likeTable2USPHS recommendations for maximum allowablefluoride in drinking waterAnnual average of maximum daily air temperature(◦C)Recommendedfluoride concentration(mg/L)Maximum allowablefluorideconcentration(mg/L) Lower Optimum Upper10–120.9 1.2 1.7 2.4 12.1–14.60.8 1.1 1.5 2.2 14.7–17.70.8 1.0 1.3 2.0 17.8–21.40.70.9 1.2 1.8 21.5–26.20.70.8 1.0 1.6 26.3–32.50.60.70.8 1.4458Meenakshi,R.C.Maheshwari/Journal of Hazardous Materials B137(2006)456–463Table3Fluoride concentration in agricultural crops and other edible items[26]Food item Fluoride concentration(mg/kg) CerealsWheat 4.6Rice 5.9MaizePulses and legumes 5.6Green gram dal 2.5Red gram dal 3.7Soyabean 4.0VegetablesCabbage 3.3Tomato 3.4Cucumber 4.1Ladyfinger 4.0Spinach 2.0Lettuce 5.7Mint 4.8Potato 2.8Carrot 4.1FruitsMango 3.7Apple 5.7Guava 5.1Nuts and oil seedsAlmond 4.0Coconut 4.4Mustard seeds 5.7Groundnut 5.1BeveragesTea60–112Aerated drinks0.77–1.44Spices and condimentsCorriander 2.3Garlic 5.0Turmeric 3.3Food from animal sourcesMutton 3.0–3.5Beef 4.0–5.0Pork 3.0–4.5Fishes 1.0–6.5OthersRock salts200.0–250.0Areca but(supari) 3.8–12.0Beetle leaf(pan)7.8–12.0Tobacco 3.2–38bone and teeth leading to developmental alternations is of clini-cal significance as they have highest amount of calcium and thus attract the maximum amount offluoride that gets deposited as calcium–fluorapatite crystals.Tooth enamel is composed prin-cipally of crystalline hydroxylapatite.Under normal conditions, whenfluoride is present in water supply,most of the ingested fluoride ions get incorporated into the apatite crystal lattice of calciferous tissue enamel during its formation.The hydroxyl ion gets substituted byfluoride ion sincefluorapatite is more stable than hydroxylapatite.Thus,a large amount offluoride gets bound in these tissues and only a small amount is excreted Table4Effects offluoride in water on human healthFluoride concentration(mg/L)Effects<1.0Safe limit1.0–3.0Dentalfluorosis(discoloration,mottling andpitting of teeth)3.0–4.0Stiffened and brittle bones and joints4.0–6.0and above Deformities in knee and hip bones andfinallyparalysis making the person unable to walk orstand in straight posture,cripplingfluorosisthrough sweat,urine and stool.The intensity offluorosis is not merely dependent on thefluoride content in water,but also on the fluoride from other sources,physical activity and dietary habits.The various forms offluorosis arising due to excessive intake offluoride are briefly discussed below(Table4)[27,28].3.1.DentalfluorosisDue to excessivefluoride intake,enamel loses its lustre.In its mild form,dentalfluorosis is characterized by white,opaque areas on the tooth surface and in severe form,it is manifestated as yellowish brown to black stains and severe pitting of the teeth. This discoloration may be in the form of spots or horizontal streaks[29].Normally,the degree of dentalfluorosis depends on the amount offluoride exposure up to the age of8–10,as fluoride stains only the developing teeth while they are being formed in the jawbones and are still under the gums.The effect of dentalfluorosis may not be apparent if the teeth are already fully grown prior to thefluoride over exposure.Therefore,the fact that an adult shows no signs of dentalfluorosis does not necessarily mean that his or herfluoride intake is within the safety limit.3.2.SkeletalfluorosisSkeletalfluorosis affects children as well as adults.It does not easily manifest until the disease attains an advanced stage.Fluo-ride mainly gets deposited in the joints of neck,knee,pelvic and shoulder bones and makes it difficult to move or walk.The symp-toms of skeletalfluorosis are similar to spondylitis or arthritis. Early symptoms include sporadic pain,back stiffness,burning like sensation,pricking and tingling in the limbs,muscle weak-ness,chronic fatigue,abnormal calcium deposits in bones and ligaments.The advanced stage is osteoporosis in long bones and bony outgrowths may occur.Vertebrae may fuse together and eventually the victim may be crippled.It may even lead to a rare bone cancer,osteosarcoma andfinally spine,major joints, muscles and nervous system get damaged.3.3.Other problemsThis aspect offluorosis is often overlooked because of the notion prevailing thatfluoride only affects bones and teeth[30]. Besides skeletal and dentalfluorosis,excessive consumption of fluoride may lead to musclefibre degeneration,low haemoglobinMeenakshi,R.C.Maheshwari/Journal of Hazardous Materials B137(2006)456–463459levels,deformities in RBCs,excessive thirst,headache,skin rashes,nervousness,neurological manifestations(it affects brain tissue similar to the pathological changes found in humans with Alzheimer’s disease),depression,gastrointestinal problems,uri-nary tract malfunctioning,nausea,abdominal pain,tingling sensation infingers and toes,reduced immunity,repeated abor-tions or still births,male sterility,etc.It is also responsible for alterations in the functional mechanisms of liver,kidney, digestive system,respiratory system,excretory system,central nervous system and reproductive system,destruction of about 60enzymes.The effects offluoride in drinking water on animals are analogous to those on human beings.The continuous use of water having highfluoride concentration also adversely affects the crop growth.3.4.Solutions to the problemA community with excessivefluoride in its water supply may meet the local MCL in one or more of several ways.Fluoride poisoning can be prevented or minimized by:ing alternate water sources.2.By improving the nutritional status of population at risk.3.By removing excessfluoride(defluoridation).3.4.1.Alternate water sourcesAlternate water sources include surface water,rainwater and low-fluoride groundwater.Since surface water is often heavily contaminated with biological and chemical pollutants,it cannot be used for drinking purposes without treatment and disinfec-tion making it too expensive and complex for application in poor communities.Rainwater is usually a much cleaner water source and may provide a low-cost simple solution.The prob-lem however is its uneven distribution limited storage capacity in communities or households.The fact thatfluoride is unevenly distributed in groundwater and its concentration keeps on chang-ing with time both vertically and horizontally,implies that every well has to be tested individually and regular monitoring has to be done,which is not always possible in rural areas.Thus the option of using alternate water sources has its own limitations.3.4.2.Better nutritionClinical data indicate that adequate calcium intake is directly associated with a reduced risk of dentalfluorosis[31].Vitamin C also safeguards against the risk[32].Though,measures to improve the nutritional status of an affected population might be an effective supplement to the technical solutions of the problem, practically it sounds non-feasible.3.4.3.Defluoridation of waterDefluoridation of drinking water is the only practicable option to overcome the problem of excessivefluoride in drink-ing water,where alternate source is not available.During the years following the discovery offluoride as the cause offluo-rosis,extensive research has been done on various methods for removal offluoride from water and wastewater.These meth-ods are based on the principle of adsorption[33],ion-exchange [34],precipitation–coagulation[35,36],membrane separation process[37,38],electrolytic defluoridation[39],electrodialysis [40–42],etc.3.4.3.1.Adsorption.Several adsorbent materials have been tried in the past tofind out an efficient and economical deflu-oridating agent.Activated alumina,activated carbon,activated alumina coated silica gel,calcite,activated saw dust,activated coconut shell carbon and activatedfly ash,groundnut shell, coffee husk,rice husk,magnesia,serpentine,tricalcium phos-phate,bone charcoal,activated soil sorbent,carbion,defluoron-1,defluoron-2,etc.,are different adsorbent materials reported in the literature[43–51].The most commonly used adsorbents are activated alumina and activated carbon.Thefluoride remov-ing efficiency of activated alumina gets affected by hardness and surface loading(the ratio of totalfluoride concentration to activated alumina dosage).Chloride does not affect the defluori-dation capacity of activated alumina.The process is pH specific, so pH of the solution should be between5.0and6.0because at pH>7,silicate and hydroxide become stronger competitor of thefluoride ions for exchange sites on activated alumina and at pH less than5,activated alumina gets dissolved in acidic environment leading to loss of adsorbing media[52].The process is highly selective but it has low adsorption capacity, poor physical integrity,requires acidification and pretreatment and its effectiveness forfluoride removal reduces after each regeneration.Mckee and Johnston1934,investigated the use of powdered activated carbon forfluoride removal and achieved good results [53].The process is pH dependent with good results only at pH 3.0or less.Therefore,the use of this material is expensive due to need of pH adjustment.Activated alumina technique for defluoridation is being prop-agated in several villages by the voluntary organizations funded by UNICEF or other agencies to provide safe drinking water. Sarita Sansthan,Udaypur,Rajasthan is disseminating the tech-nique with the practical assistance of UNICEF by providing a bucket(approximately20L capacity)fitted with a microfilter at the bottom containing5kg of activated alumina.3.4.3.1.1.Advantages.•The process can removefluoride up to90%.•Treatment is cost-effective.3.4.3.1.2.Limitations.•The process is highly dependent on pH and works best only in a narrow pH range(5–6).•High concentration of total dissolved salts(TDS)can result in fouling of the alumina bed.•Presence of sulfate,phosphate or carbonate results in ionic competition.•The process has low adsorption capacity,poor integrity and needs pretreatment.•The regeneration is required after every4–5months and effec-tiveness of adsorbent forfluoride removal reduces after each regeneration.460Meenakshi,R.C.Maheshwari/Journal of Hazardous Materials B137(2006)456–463•Disposal offluoride laden sludge and concentrated regenerant is also a problem.3.4.3.2.Ion-exchange.Fluoride can be removed from water supplies with a strongly basic anion-exchange resin containing quarternary ammonium functional groups.The removal takes place according to the following reaction:Matrix-NR3+Cl−+F−→Matrix-NR3+F−+Cl−Thefluoride ions replace the chloride ions of the resin.This process continues until all the sites on the resin are occupied. The resin is then backwashed with water that is supersaturated with dissolved sodium chloride salt.New chloride ions then replace thefluoride ions leading to recharge of the resin and starting the process again.The driving force for the replacement of chloride ions from the resin is the stronger electronegativity of thefluoride ions.3.4.3.2.1.Advantages.•Removesfluoride up to90–95%.•Retains the taste and colour of water intact.3.4.3.2.2.Limitations.•Efficiency is reduced in presence of other ions like sulfate, carbonate,phosphate and alkalinity.•Regeneration of resin is a problem because it leads tofluoride rich waste,which has to be treated separately beforefinal disposal.•The technique is expensive because of the cost of resin,pre-treatment required to maintain the pH,regeneration and waste disposal.•Treated water has a very low pH and high levels of chloride.3.4.3.3.Coagulation–precipitation.Lime and alum are the most commonly used coagulants.Addition of lime leads to pre-cipitation offluoride as insoluble calciumfluoride and raises the pH value of water upto11–12.Ca(OH)2+2F−→CaF2+2OH−As lime leaves a residue of8.0mg F−/L,it is used only in conjunction with alum treatment to ensure the properfluoride removal[54–56].As afirst step,precipitation occurs by lime dosing which is followed by a second step in which alum is added to cause coag-ulation.When alum is added to water,essentially two reactions occur.In thefirst reaction,alum reacts with some of the alka-linity to produce insoluble aluminium hydroxide[Al(OH)3].In the second reaction,alum reacts withfluoride ions present in the water.The bestfluoride removal is accomplished at pH range of 5.5–7.5[57].3.4.3.3.1.Advantages.•The Nalgonda technique of defluoridation is based on com-bined use of alum and lime in a two-step process and has been claimed as the most effective technique forfluoride removal [58,59].•Under Rajiv Gandhi Drinking Water Mission,severalfill and draw(F&D)type and handpump attached(HPA)plants based on Nalgonda technique have come up in rural areas for which design and technology has been developed by NEERI,Nag-pur.3.4.3.3.2.Limitations.After having10years experience with these plants,the following serious drawbacks have been experienced:•The process removes only a smaller portion offluoride (18–33%)in the form of precipitates and converts a greater portion of ionicfluoride(67–82%)into soluble aluminium fluoride complex ion,and therefore this technology is erro-neous.Also,as the soluble aluminiumfluoride complex is itself toxic,adoption of Nalgonda technique for defluorida-tion of water is not desirable[60].•Due to use of aluminium sulfate as coagulant,the sulfate ion concentration increases tremendously and in few cases,it crosses the maximum permissible limit of400mg/L,which causes cathartic effect in human beings.•The residual aluminium in excess of0.2mg/L in treated water causes dangerous dementia disease as well as pathophysiolog-ical,neurobehavioural,structural and biochemical changes. It also affects musculoskeletal,respiratory,cardiovascular, endocrine and reproductive systems[61].•Due to organoleptic reasons,users do not like the taste of treated water.•Regular analysis of feed and treated water is required to calcu-late the correct dose of chemicals to be added,because water matrix keeps on changing with time and season as evident from our earlier studies conducted in laboratory.•Maintenance cost of plant is very high.On an average as experienced in the recent years,a plant of10,000L per day capacity requires Rs.3000every month on maintenance.•The process is not automatic.It requires a regular attendant for addition of chemicals and looking after treatment process.•Large space is required for drying of sludge.•Silicates have adverse effect on defluoridation by Nalgonda technique.Temperature also affects the defluoridation capac-ity.3.4.3.4.Membrane process.Although various conventional techniques of water purification described earlier are being used at present to solve the problem of groundwater pollution, none of them is user-friendly and cost-effective technique due to some or the other limitation and has either no or very long pay back period.In the recent years,RO membrane process has emerged as a preferred alternative to provide safe drinking water without posing the problems associated with other conventional methods.RO is a physical process in which the contaminants are removed by applying pressure on the feed water to direct it through a semipermeable membrane.The process is the reverse of natural osmosis as a result of the applied pressure to the concentrated side of the membrane,which overcomes the natural osmotic pressure.RO membrane rejects ions based on size and electrical charge.The factors influencing theMeenakshi,R.C.Maheshwari/Journal of Hazardous Materials B137(2006)456–463461 Table5Comparative analysis of various techniques forfluoride removalSample no.Initialfluoride concentration(mg/L)Fluoride concentration after treatment(mg/L)Activated alumina Activated saw dust Nalgonda Reverse osmosis 1 4.2 1.13(73.10) 1.42(66.19) 1.32(68.57)0.32(92.38) 27.8 1.96(74.87) 2.32(70.26) 2.24(71.29)0.63(91.93) 38.6 2.23(74.07) 2.56(70.23) 2.47(71.30)0.78(90.93) 49.3 2.11(77.31) 2.42(73.98) 2.31(71.16)0.88(90.54) 58.2 2.17(73.54) 2.43(70.37) 2.34(71.46)0.77(90.61)6 6.8 1.81(73.38) 2.16(68.24) 1.95(71.32)0.56(91.76) Values in parentheses show the percentfluoride removal.membrane selection are cost,recovery,rejection,raw water characteristics and pretreatment.Efficiency of the process is governed by different factors such as raw water characteristics, pressure,temperature and regular monitoring and maintenance, etc.There are two types of membranes that can removefluoride from water:NF and RO.NF is a relatively low pressure process that removes primarily the larger dissolved solids as compared to RO.Conversely,RO operates at higher pressures with greater rejection of all dissolved solids.Fluoride removal efficiencies upto98%by membrane processes have been documented by many researchers.In the past,the use of membrane technology for water treat-ment,particularly for drinking water production had been con-sidered uneconomical in comparison with conventional means, but in the recent years the increased demand and contamina-tion of water,rise in water quality standards and the prob-lems associated with other methods have led to reconsideration of membrane technology for water purification.The progres-sive technical improvements in design and materials of the membranes have made the water treatment process econom-ically competitive and highly reliable.Also,the capital and operational costs of RO plant go on decreasing with increas-ing plant capacity[62].Thus with improved management,this new technology for drinking water production might be the best option.Furthermore,membrane processes present sev-eral advantages as compared with other treatment methods [63].3.4.3.4.1.Advantages.•The process is highly effective forfluoride removal.Mem-branes also provide an effective barrier to suspended solids, all inorganic pollutants,organic micropollutants,pesticides and microorganisms,etc.•The process permits the treatment and disinfection of water in one step.•It ensures constant water quality.•No chemicals are required and very little maintenance is needed.•Life of membrane is sufficiently long,so problem of regener-ation or replacement is encountered less frequently.•It works under wide pH range.•No interference by other ions is observed.•The process works in a simple,reliable automated operat-ing regime with minimal manpower using compact modular model.3.4.3.4.2.Limitations.•It removes all the ions present in water,though some minerals are essential for proper growth,remineralization is required after treatment.•The process is expensive in comparison to other options.•The water becomes acidic and needs pH correction.•Lot of water gets wasted as brine.•Disposal of brine is a problem.•The performance of all the above processes has been tested in the laboratory.A comparative analysis of thefluoride removal by various processes is presented in Table5.4.ConclusionThe literature survey and the laboratory experiments have indicated that each of the discussed techniques can removefluo-ride under specified conditions.Thefluoride removal efficiency varies according to many site-specific chemical,geographical and economic conditions,so actual applications may vary from the generalizations made.Any particular process,which is suit-able at a particular region may not meet the requirements at some other place.Therefore,any technology should be tested using the actual water to be treated before implementation in the field.References[1]A.Kass,Y.Yechieli Gavrieli,A.Vengosh,A.Starinsky,The impact offreshwater and wastewater irrigation on the chemistry of shallow ground-water:a case study from the Israeli Coastal aquifer,J.Hydrol.300(1–4) (2005)314–331.[2]C.Amina,L.K.Lhadi,A.Younsi,J.Murdy,Environmental impact ofan urban landfill on a coastal aquifer,J.Afr.Earth Sci.39(3–5)(2004) 509–516.[3]O.Oren,Y.Yechieli,J.K.Bohlke,A.Dody,Contamination of groundwaterunder cultivatedfields in an arid environment,Central Arava Valley,Israel, J.Hydrol.290(3/4)(2004)312–328.[4]F.Anwar,Assessment and analysis of industrial liquid waste and sludgedisposal at unlined landfill sites in arid climate,Waste Manage.23(9) (2003)817–824.。
复合酶法提取槐花多糖的工艺优化及其抗氧化活性
任晓莉,杨璐,乔鹏,等. 复合酶法提取槐花多糖的工艺优化及其抗氧化活性[J]. 食品工业科技,2024,45(7):8−14. doi:10.13386/j.issn1002-0306.2023070216REN Xiaoli, YANG Lu, QIAO Peng, et al. Optimization of Extraction Process of Polysaccharide from Sophora japonica by Compound Enzyme Method and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2024, 45(7): 8−14. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070216· 特邀主编专栏—食品中天然产物提取分离、结构表征和生物活性(客座主编:杨栩、彭鑫) ·复合酶法提取槐花多糖的工艺优化及其抗氧化活性任晓莉*,杨 璐,乔 鹏,缪奕锴,杨懿昂,代秋红,张贤德(太原工业学院环境与安全工程系,山西太原 030008)摘 要:目的:采用复合酶法提取槐花多糖,对提取工艺进行优化,并评价其体外抗氧化活性。
方法:通过单因素实验考察复合酶添加量、pH 、复合酶比例和酶解时间对得率的影响,在单因素实验基础上,采用响应面法确定槐花多糖的最佳提取参数,并以V C 为对照,通过测定槐花多糖对DPPH·和ABTS +·的清除率及总还原力,考察所提取的槐花多糖的抗氧化活性。
结果:复合酶法提取槐花多糖的最佳提取参数为:复合酶添加量23.8 mg/g ,pH4.8,果胶酶与纤维素酶比例0.912:1,该工艺下槐花多糖得率为10.71%,所提取的槐花多糖对DPPH·和ABTS +·均表现出较好的清除能力,当槐花多糖溶液浓度为2.8 mg/mL 时,对DPPH·和ABTS +·的清除率分别达到同浓度下V C 的94.19%和99.79%,总还原力达到V C 的75.99%。
生物酶与季铵盐的复合物
生物酶与季铵盐的复合物英文回答:Enzymes are proteins that act as catalysts inbiological reactions. They are highly specific and can speed up chemical reactions by lowering the activation energy required for the reaction to occur. Enzymes can bind to specific molecules called substrates and convert them into products.Quaternary ammonium salts, also known as quats, are organic compounds that contain a positively charged nitrogen atom and four organic groups attached to it. They are widely used as disinfectants, surfactants, and fabric softeners due to their antimicrobial and surface-active properties.The formation of a complex between enzymes and quaternary ammonium salts can have various effects. In some cases, the complex formation can enhance the activity ofthe enzyme, while in others, it can inhibit or even denature the enzyme.One example of a complex between an enzyme and a quaternary ammonium salt is the interaction between cholinesterase and the pesticide paraoxon. Cholinesteraseis an enzyme that breaks down acetylcholine, a neurotransmitter involved in nerve signal transmission. Paraoxon is an organophosphate pesticide that irreversibly inhibits cholinesterase by forming a covalent bond with the enzyme's active site. This complex formation leads to the accumulation of acetylcholine, causing overstimulation of nerve cells and ultimately leading to paralysis or death.Another example is the complex between the enzyme trypsin and the quaternary ammonium salt benzalkonium chloride. Trypsin is a protease enzyme that cleaves peptide bonds in proteins. Benzalkonium chloride, commonly used as a disinfectant, can inhibit trypsin activity by binding to the enzyme's active site and preventing substrate binding. This complex formation can be useful in controlling trypsin activity in certain applications, such as in the productionof protein-based drugs.中文回答:酶是一种在生物反应中起催化剂作用的蛋白质。
两种常用适配体的纳米金比色法快速检测牛奶中黄曲霉毒素M1的评价研究
苏柳,贺伟华,张干,等. 两种常用适配体的纳米金比色法快速检测牛奶中黄曲霉毒素M 1的评价研究[J]. 食品工业科技,2024,45(8):284−292. doi: 10.13386/j.issn1002-0306.2023050332SU Liu, HE Weihua, ZHANG Gan, et al. Evaluation of Gold Nanoparticles Colorimetric Sensing Based on Two Commonly Aptamer for Rapid Detecting Aflatoxin M 1 in Milk[J]. Science and Technology of Food Industry, 2024, 45(8): 284−292. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050332· 分析检测 ·两种常用适配体的纳米金比色法快速检测牛奶中黄曲霉毒素M 1的评价研究苏 柳1,贺伟华1, +,张 干1,陈爱亮2,章钢刚1,赖晓翠1,邓省亮1,*(1.江西省科学院微生物研究所,江西南昌 330096;2.中国农业科学院农业质量标准与检测技术研究所,北京 100081)摘 要:目的:建立基于不同序列长度适配体的纳米金(Gold nanoparticles ,AuNPs )比色传感法快速定量检测牛奶中的黄曲霉毒素M 1(Aflatoxin M 1,AFM 1),并评价目前文献报道中常用的21(A21)和72个碱基(A72)长度的AFM 1适配体在实际样品中的检测性能。
方法:采用柠檬酸钠还原法制备AuNPs 溶液,加入AFM 1适配体及AFM 1标准品后,适配体与AFM 1特异性结合形成特殊三维结构,随着NaCl 溶液加入,AuNPs 溶液的稳定性被破坏而发生聚集,导致溶液颜色变化,通过测定AuNPs 溶液的吸光值和吸收光谱定量检测AFM 1。
正庚烷氧化A comprehensive modeling study of n-heptane oxidation
A Comprehensive Modeling Study of n-Heptane OxidationH.J.CURRAN,*P.GAFFURI,W.J.PITZ,AND C.K.WESTBROOKLawrence Livermore National Laboratory,Livermore,CAA detailed chemical kinetic mechanism has been developed and used to study the oxidation of n-heptane inflow reactors,shock tubes,and rapid compression machines.Over the series of experiments numerically investi-gated,the initial pressure ranged from1–42atm,the temperature from550–1700K,the equivalence ratio from 0.3–1.5,and nitrogen-argon dilution from70–99%.The combination of ignition delay time and species composition data provide for a stringent test of the chemical kinetic mechanism.The reactions are classed into various types,and the reaction rate constants are given together with an explanation of how the rate constants were obtained.Experimental results from the literature of ignition behind reflected shock waves and in a rapid compression machine were used to develop and validate the reaction mechanism at both low and high temperatures.Additionally,species composition data from a variable pressureflow reactor and a jet-stirred reactor were used to help complement and refine the low-temperature portions of the reaction mechanism.A sensitivity analysis was performed for each of the combustion environments.This analysis showed that the low-temperature chemistry is very sensitive to the formation of stable olefin species from hydroperoxy-alkyl radicals and to the chain-branching steps involving ketohydroperoxide molecules.©1998by The Combustion InstituteINTRODUCTIONThere is continued interest in developing a better understanding of the oxidation of large hydrocarbon fuels over a wide range of operat-ing conditions.This interest is motivated by the need to improve the efficiency and performance of currently operating combustors and reduce the production of pollutant species emissions generated in the combustion process.This study particularly focuses on the effect of elevated pressures on the oxidation of n-heptane.Many important practical combustion systems such as spark-ignition,diesel and gas-turbine engines operate at pressures well above1atm.n-Hep-tane is a primary reference fuel(PRF)for octane rating in internal combustion engines and has a cetane number of approximately56, which is very similar to the cetane number of conventional diesel fuel.Therefore,a better understanding of n-heptane oxidation kinetics is useful in studies of engine knock and autoigni-tion.Recent experimental studies of n-heptane oxidation have focused on shock tubes[1–5], jet-stirred reactors[6–8]performed under sta-tionary conditions,rapid compression machines [9–12],engines[13–19],plugflow reactors[20–22],and jet-stirredflow reactors[23,24]in which a dynamic behavior is observed.All of these systems exhibit phenomena including self-ignition,coolflame,and negative temperature coefficient(NTC)behavior.Furthermore,vari-ation in pressure from5–40bar changes the temperature range over which the NTC region occurs.Recent modeling studies of the premixed systems such as stirred reactors and shock tubes cited above[2–6]have helped in the develop-ment of detailed chemical kinetic mechanisms that describe n-heptane oxidation.These publi-cations have been complemented by the work of Chevalier et al.[25,26],Muller et al.[27], Foelsche et al.[28],and Lindstedt and Maurice [29].In addition,Bui-Pham and Seshadri[30] carried out a numerical study of an n-heptane diffusionflame.More recently,Ranzi et al.[32] have used a semi-detailed chemical kinetic model to simulate n-heptane pyrolysis and oxi-dation.In addition,this semi-detailed model was used to simulate the oxidation of primary reference fuel(n-heptane and2,2,4-trimethyl-pentane)mixtures[22].Coˆme et al.[33]have used a computer package to generate chemical kinetic mechanisms for n-heptane and iso-oc-tane.In this study we include all of the reactions known to be pertinent to both high-and low-temperature kinetics.We show how the detailedCorresponding author.Current address of H.J.Curran,L-407,Lawrence Liver-more National Laboratory,Livermore,CA94550.COMBUSTION AND FLAME114:149–177(1998)©1998by The Combustion Institute0010-2180/98/$19.00 Published by Elsevier Science Inc.SSDI0010-2180(97)00282-4kinetic model reproduces the measured results in each type of experiment,including the fea-tures of the NTC region.We discuss the specific classes of elementary reactions and reaction pathways relevant to the oxidation process,how we arrived at the rate constants for each class of reaction,and indicate which reactions are the most important in consuming the fuel at both low and high temperature.In addition,a sensi-tivity analysis was carried out on each set of experimental results by changing the rate con-stants for different classes of reaction in the kinetic mechanism.The results of this analysis indicate the relative importance of each class of reaction and also the variation in contribution of these classes of reactions to the changing conditions of the experiments.MODEL FORMULATIONComputer modeling of n-heptane oxidation was performed using the HCT(hydrodynamics, chemistry,and transport)program[31],which solves the coupled chemical kinetic and energy equations and permits the use of a variety of boundary and initial conditions for reactive systems,depending on the needs of the partic-ular system being examined.The present de-tailed reaction mechanism was constructed based on the hierarchical nature of hydrocar-bon–oxygen systems.The mechanism was built in a stepwise fashion starting with small hydro-carbons and progressing to larger ones.Much of this work has been documented previously[34–38]but has required extensive refinements.A semi-detailed kinetic scheme developed by Ranzi et al.[32,39]in which both the low-and the high-temperature reaction submechanisms are reduced to a lumped kinetic model involving a limited number of intermediate steps was employed.This lumped reaction model was found to be extremely valuable in identifying portions of the detailed mechanism that were especially sensitive and that required modifica-tion and improvement.To cover the complete range of temperature and pressure typical of n-heptane oxidation,it was important to include both the low-and the high-temperature mechanisms.At higher tem-peratures,unimolecular fuel and alkyl radical species decomposition and isomerization reac-tions are especially important,while,at low temperatures,H-atom abstraction from the fuel molecule and addition of alkyl radicals to mo-lecular oxygen followed by reactions of the alkylperoxy radicals dominate the oxidation mechanism.The low-temperature submechanism that was developed previously[35–38]has undergone several major changes that were necessary to explain the combustion of n-heptane in the temperature range550–900K.A-decomposi-tion reaction path for hydroperoxy-alkyl radi-cals,leading to the formation of smaller olefins and aldehydes,was included.This step has helped explain the selectivity for lower alkenes [7,8,12]and also increases the number of chain propagation pathways that compete with the chain-branching channel in the NTC region. Furthermore,ketohydroperoxide species have been identified during the oxidation of n-heptane in a motored CFR engine[15].Con-sequently,we have added a pathway leading to the formation of ketohydroperoxide compounds from the isomerization reactions of O˙2QOOH radicals.These ketohydroperoxide species sub-sequently decompose,producing one other hy-droxyl radical(chain-branching step)in addi-tion to other oxygenated compounds.The inclusion of this step has had a large influence on the reproduction of the observed NTC be-havior and two-stage ignition of the fuel.The lumped reaction model was particularly valu-able in identifying this reaction pathway as being necessary in the modeling of alkane fuels. Finally,the THERM program[40]of Ritter and Bozzelli,which uses group additivity rules developed by Benson[41],was used to evaluate thermochemical quantities for all chemical spe-cies for which there were no available data.In addition to improving the specific heats and enthalpies of formation for many C7com-pounds,it was found that reverse rate constants of many reactions in the low-temperature re-gime were quite important,and improved ther-modynamic parameters for these species pro-vided better reverse reaction rate constants. H/C/O groups and bond dissociation groups were updated based on recent work by Bozzelli and coworkers[42].150H.J.CURRAN ET AL.CLASSES OF REACTIONSWe have developed our chemical kinetic model in a systematic way.The oxidation of any fuel takes place through a series of steps.For exam-ple,at both low and high temperature,n-hep-tane undergoes H-atom abstraction,leading to the formation of four possible,structurally dis-tinct alkyl radicals.At high temperatures these radicals decompose via-scission to yield a smaller olefin and another radical species.How-ever,at low temperatures,these four alkyl rad-icals undergo addition to O2leading to the formation of four heptylperoxy radicals.There-fore,we can categorize each step in the oxida-tion process as a class of reaction,including all the possible reactions taking place.These classes of reactions are listed below and indicate the com-plexity of the model.The complete reaction mech-anism for n-heptane oxidation included2450ele-mentary reactions among550chemical species. The entire mechanism is not represented here due to its length,but we discuss below its contents,and a complete copy can be obtained from the authors in either printed or electronic form.We have found many reaction types to be important,and particular attention and care have been taken in developing rate constant expressions for these reaction classes.However, not all reactions have been found to be impor-tant,and we have at times been expedient in our treatment of certain reaction types.For exam-ple,in our treatment of H-atom abstraction from C7alkene species,we assume only one alkenyl radical is produced,which is taken as an “average”over the species possible for n-hep-tane.Furthermore,we have also simplified alk-enyl consumption to consist only of unimolecu-lar decomposition to products we have selected as being reasonable for the fuel.Thus in n-heptane,alkenyl radical decomposition is as-sumed to lead to allyl radical and olefinic prod-ucts.This treatment is very approximate,and further attention may be warranted,but this has proven adequate for our current applications. The major classes of elementary reactions con-sidered in the present mechanism include the following:1.Unimolecular fuel decomposition2.H-atom abstraction from the fuel3.Alkyl radical decomposition4.Alkyl radicalϩO2to produce olefinϩHO˙2directly5.Alkyl radical isomerization6.Abstraction reactions from olefin by O˙H,H˙,O˙,and C˙H37.Addition of radical species to olefin8.Alkenyl radical decomposition9.Olefin decomposition10.Addition of alkyl radicals to O211.R˙ϩRЈO˙2ϭRO˙ϩRЈO˙12.Alkyl peroxy radical isomerization(RO˙2ºQ˙OOH)13.RO˙2ϩHO˙2ϭRO2HϩO214.RO˙2ϩH2O2ϭRO2HϩHO˙215.RO˙2ϩCH3O˙2ϭRO˙ϩCH3O˙ϩO216.RO˙2ϩRЈO˙2ϭRO˙ϩRЈO˙ϩO217.RO2HϭRO˙ϩO˙H18.RO˙decomposition19.Q˙OOHϭQOϩO˙H(cyclic ether forma-tion via cyclization of diradical)20.Q˙OOHϭolefinϩHO˙2(radical sitetoOOH group)21.Q˙OOHϭolefinϩcarbonylϩO˙H(radicalsite␥to OOH group)22.Addition of Q˙OOH to O223.Isomerization of O˙2QOOH and formationof ketohydroperoxide and O˙H24.Decomposition of ketohydroperoxide toform oxygenated radical species and O˙H 25.Cyclic ether reactions with O˙H and HO˙2 The naming conventions used above are R˙and RЈ,denoting alkyl radicals or structures, and Q,denoting C n H2n species or structures. For each of these classes of reactions we use the same reaction rate constant for analogous oc-currences in different molecules.Thus,we as-sume that the abstraction of a tertiary H atom by reaction with O˙H radicals has exactly the same rate in2-methyl butane,2-methyl pentane, 3-methyl pentane,and in iso-octane.Corre-spondingly,the total rate of tertiary H-atom abstraction by O˙H in2,3-dimethyl butane and in 2,4-dimethyl pentane is twice that in2-methyl pentane,since the two former fuels have two such H atoms at tertiary sites.The n-heptane mechanism contains C4,C5,and C6submecha-nisms.We treat all of the different reaction classes provided above in exactly the same way regardless of whether the fuel is n-butane,n-151MODELING STUDY OF n-HEPTANE OXIDATIONpentane,n-hexane,or n-heptane.Our treat-ment of these reaction classes in described in the following sections.HIGH-TEMPERATURE MECHANISM Reactions in classes1–9are sufficient to simu-late many high-temperature applications of n-heptane oxidation.We have made a number of ad hoc assumptions and approximations that may not be suitable for some problems involving alkene and alkyne fuels,and further analysis is needed to refine details for these fuels.How-ever,under the conditions of this study,n-heptane oxidation is relatively insensitive to these assumptions.Reaction Type1:Unimolecular Fuel DecompositionThese reactions produce two alkyl radicals or one alkyl radical and one H atom.Because the paths producing H atoms have very high activa-tion energies,they are only important in the reverse direction,where they serve as sinks of H atoms.Type1reactions serve as initiation steps and as fuel consumption reactions but only at relatively high temperatures,such as those found in shock tubes.We calculate the rate constant expressions for unimolecular decomposition of n-heptane fuel from the reverse reaction,the recombination of two radical species to form the stable parent fuel,and from microscopic reversibility.For products of alkylϩH atom,we assume a rate constant for recombination of1ϫ1014cm3 molϪ1sϪ1,based on the recommendation of Allara and Shaw[43].There is very little infor-mation available on the rate of this reaction for C2alkyl radicals and larger.For decompositions where the smallest product is C˙H3,we assume the reverse recombination rate to be1.0ϫ1013 cm3molϪ1sϪ1,similar to that recommended by Baulch et al.[44]for C˙H3ϩC˙H3ϭC2H6. When the smallest product is an alkyl radical such as ethyl or larger,we assume the recombi-nation rate constant to be8.0ϫ1012cm3molϪ1 sϪ1.Reaction Type2:H-Atom AbstractionAt both low and high temperatures,H-atom abstraction takes place at both primary and secondary sites of n-heptane,which leads to the formation of four distinct heptyl radicals.There are no tertiary sites on the n-heptane molecule, but we have included these rate constant expres-sions so that we can provide a complete set,and these will be used in modeling iso-octane oxida-tion.We assume that the rate constant for abstrac-tion at any particular site(1°,2°,or3°)to be equal to that at the same type of site in other molecules.For example,we chose the rate constant for H-atom abstraction by O˙H radicals at both primary and secondary sites to be iden-tical to those recommended by Droege and Tully[45]for propane fuel.The rate constant for tertiary H-atom abstraction by O˙H radicals was taken from a similar study by the same authors[46]with isobutane fuel.We summarize these rate constant expressions in Table1,and calculate the reverse rate constants from ther-mochemistry.Reaction Type3:Alkyl Radical DecompositionAlkyl radical decomposition is important only at relatively high temperatures(TՆ850K)under the conditions of this study,as the addition of alkyl radicals to molecular oxygen,even though a bimolecular reaction,is faster than-scission due to the relatively high activation energy barriers for alkyl radical decomposition(there is no energy barrier for the addition to O2).Gen-erally,we have chosen products based on the principle that-scission will be the dominant decomposition path for alkyl radicals.In many cases there are two or more pathways possible for an alkyl radical,with different products,and all such paths have been included in the present mechanism.Previously,we treated this type of reaction in the forward direction,estimating the rate con-stant for each-scission by analogies with sim-ilar reactions.However,because alkyl radical -scission is endothermic,we now calculate the rate constant in the reverse,exothermic direc-tion,i.e.,the addition of an alkyl radical(or H˙atom)across the double bond of an alkene.In152H.J.CURRAN ET AL.this way we avoid the additional complexity of the enthalpy of reaction,allowing the forward,-scission rate constant to be calculated from thermochemistry.Rate constants for the addition of radicals across a double bond are reasonably well known and are very similar depending on (1)the site of addition (terminal or internal C atom)and (2)the type of radical adding on.We use rate constants for these addition reactions based on the recommendations of Allara and Shaw [43].Typically,the rate of addition of a H˙atom across a double bond has a pre-exponentialᏭ-factor of 1ϫ1013cm 3mol Ϫ1s Ϫ1with anactivation energy of 1200cal/mol if the H˙atom adds to the terminal C atom of the alkene and2900cal/mol if the H˙atom adds to an internal C atom.The rate constant for the addition of an alkyl radical has a lower Ꮽ-factor and higher activation energy than for the addition of a H atom.For the addition of an alkyl radical,the Ꮽ-factor is approximately 8.5ϫ1010cm 3mol Ϫ1s Ϫ1with an activation energy of approximately 7800cal/mol if addition occurs at the terminal C atom and 10,600cal/mol if addition occurs at an internal C atom.We assume these reactions areTABLE 1Rate Constant Expressions for H-Atom Abstraction from the Fuel (cm 3-mol-s-cal Units)Radical Site Rate expression per H atomReference Ꮽn Ᏹa H ˙Primary9.33ϫ106 2.07,70047H ˙Secondary 4.55ϫ106 2.05,00047H ˙Tertiary 1.26ϫ10140.07,30048O ˙H Primary 1.75ϫ1090.971,59045O ˙H Secondary 2.34ϫ107 1.61Ϫ3545O ˙H Tertiary 5.73ϫ10100.516346O ˙Primary 7.33ϫ105 2.45,50049O ˙Secondary 2.35ϫ105 2.52,23049O ˙Tertiary 1.10ϫ10130.03,28049C ˙H 3Primary 2.17ϫ10110.011,60050C ˙H 3Secondary 2.00ϫ10110.09,50050C ˙H 3Tertiary 1.00ϫ10110.07,90051HO ˙2Primary 1.34ϫ10120.019,40052a HO ˙2Secondary 1.22ϫ10120.017,00052a HO ˙2Tertiary 2.16ϫ10120.014,40052a CH 3O ˙Primary 5.27ϫ10100.07,00053CH 3O ˙Secondary 5.48ϫ10110.05,00053CH 3O ˙Tertiary 1.90ϫ10100.02,80053O 2Primary 4.17ϫ10120.049,00053b O 2Secondary 1.00ϫ10130.047,60053b O 2Tertiary 2.00ϫ10130.041,30053b C ˙2H 5Primary 1.67ϫ10100.013,40043C ˙2H 5Secondary 2.50ϫ10100.010,40043C ˙2H 5Tertiary 1.00ϫ10110.07,90043C ˙2H 3Primary 1.67ϫ10110.018,00054C ˙2H 3Secondary 2.00ϫ10110.016,80054C˙2H 3Tertiary 2.00ϫ10110.014,30054CH 3O ˙2Primary 2.02ϫ10120.020,43055c CH 3O ˙2Secondary 2.02ϫ10120.017,70055c CH 3O ˙2Tertiary 2.00ϫ10120.014,00055c RO ˙2Primary 2.02ϫ10120.020,43055c RO ˙2Secondary 2.00ϫ10120.017,70055c RO˙2Tertiary2.00ϫ10120.016,00055ca Ꮽ-factors adjusted from original values of 1.0ϫ1012.bAs recommended by [53],Ᏹa Ϸ⌬H .Overall Ꮽ-factor of 4.0ϫ1013[53]was partitioned between 1°,2°,and 3°.c Analogy with RH ϩHO˙2.Ꮽ-factor has been adjusted down from 1.8ϫ1012.153MODELING STUDY OF n -HEPTANE OXIDATIONin their high-pressure limit for the conditions considered in this study.Reaction type 4:Alkyl radical ؉O 2؍olefin ؉HO˙2The reaction of alkyl radicals with O 2proceedsthrough many reaction channels.Most of these channels can be represented by the addition ofan alkyl radical,R˙,to O 2,reaction type 10,followed by alkylperoxy,RO˙2,radical isomeriza-tion to a hydroperoxy-alkyl radical,Q˙OOH,reaction type 12,and subsequent decomposition or further addition of the hydroperoxy-alkyl radical to O 2,reaction types 19–22.Reaction type 4represents the only chemically activatedchannel of the R˙ϩO 2reaction system that is considered in the reaction mechanism,proceed-ing through a vibrationally excited alkylperoxycomplex,RO ˙*2,and leads to the formation of an olefin and HO ˙2radical.R ˙ϩO 23RO ˙*23olefin ϩHO ˙2The reaction mechanism leading to the forma-tion of conjugate olefin from alkyl plus molec-ular oxygen is a topic of considerable currentresearch.Quelch and coworkers [56]have pro-posed that C 2H 5ϩO 2reacts through a cyclic transition state and then proceeds to ethyleneplus hydroperoxy radical,HO˙2,through a con-certed elimination.This proposed reaction se-quence does not proceed through the hydroper-oxy-alkyl radical,Q˙OOH.This is in agreement with the earlier proposals of Walker and co-workers [57,58],in which they suggested that a pathway involving a cyclic quasi-stable structure must exist,without the prior formation of C 2H 5O 2,which can either decompose back into C 2H 5ϩO 2or unimolecularly decompose intoC 2H 4ϩHO˙2.However,Wagner et al.[59]argue that,were this cyclic intermediate to exist,HO˙2would also react with C 2H 4forming C 2H 5ϩO 2to a significant extent instead of exclu-sively forming C˙H 2CH 2O 2H,followed by the subsequent formation of ethylene oxide,C 2H 4O ϩO˙H.No evidence of such behavior was observed by Walker and co-workers.Thus,questions concerning the kinetic behavior of theC 2H 5ϩO 23C 2H 4ϩHO˙2reaction sequence remain.However,all proposed alkyl radical plusmolecular oxygen reactions do lead to the for-mation of the conjugate olefin and HO˙2.Koert et al.[60]showed that only about 10%of the propene produced from propane oxida-tion occurs through reaction type 4,i.e.,addi-tion of an alkyl radical to O 2to form RO ˙*2,isomerization of RO ˙*2to QOOH*,and dissoci-ation of QOOH*to olefin and HO˙2radical,over the temperature range 650–800K and at 10–15atm.The remaining 90%of the propene is formed through the addition of the alkyl radical to O 2,isomerization of propyl-peroxy radical to hydroperoxy-propyl radicals,and sub-sequent decomposition to form propene plus HO˙2.C ˙3H 7ϩO 23C 3H 7O ˙*2C 3H 7O ˙*23C 3H 7O ˙2C 3H 7O˙23C ˙3H 6OOH C˙3H 6OOH 3C 3H 6ϩHO ˙2It is expected that the contribution of reaction type 4will decrease significantly with increasing number of carbon atoms in the alkyl radical.These alkyl radical plus O 2chemical activation reactions put approximately 34.0kcal mol Ϫ1of energy into the molecule,which is quickly dis-tributed to all the possible vibrational modes.There are 3N -6modes of vibration possible for a non-linear molecule,where N is the number of atoms in the molecule.Thus,as the number of atoms in a molecule increases,the probability that a critical number of quanta will reside in the one vibrational mode needed for reaction will decrease significantly.Alternatively,the RO ˙*2radicals can undergo collisional stabiliza-tion,which occurs nearly 100%of the time at the high pressure (13.5–40.0bar)and low tem-perature (550–800K)conditions investigated in this study.When the temperature greatly ex-ceeds 800K,the equilibrium of the R˙ϩO 2ϭRO˙2reaction favors R ˙ϩO 2,and large alkyl radicals (R˙)are mostly consumed by decompo-sition (reaction type 3).For these reasons,we have not included reaction type 4in our mech-anism for alkyl radical containing more than four carbon atoms.We have obtained good agreement between experimental and computa-154H.J.CURRAN ET AL.tional results for both C5and C6species using this assumption[61–63].Reaction Type5:Alkyl Radical IsomerizationAlkyl radicals can transfer H atoms from one site to the radical site at rates that depend on the type of C-H bond(primary,secondary,or tertiary)broken and the ring strain energy bar-rier involved.This process has been well known for many years and was summarized by Benson [41].The rate constants for isomerization are described in terms of the number of atoms in the transition state ring structure(including the H atom)and the type of site at which the transferred H atom was initially located.Thus, we estimate the activation energy,Ᏹa,using the expression,Ᏹaϭ⌬H rxnϩring strainϩE abst(1) where⌬H rxn is taken to be the enthalpy ofreaction and is only included if the reaction is endothermic.The activation energy for abstrac-tion is determined,following the analysis of Bozzelli and Pitz[64],from an Evans-Polanyi plot,E abst vs.⌬H rxn(taken in the exothermic direction)of similar H atom abstraction reac-tions,RHϩR˙ЈϭR˙ϩRЈH,leading to the following expression:E abstϭ12.7ϩ͑⌬H rxnϫ0.37͒TheᏭ-factors were obtained using RADI-CALC[65],a computer code that implements transition state theory.RADICALC calculates the change in entropy of the radical to the transition state due to loss or gain of internal rotors,of specific vibrations,and of optical isomers.A more in-depth description of the use of RADICALC has been published by Bozzelli and Pitz[64].We consider only H-atom trans-fers,excluding C˙H3or larger radical transfers. The rate constants employed for heptyl radical isomerizations are summarized in Table2.Reaction Type6:H-Atom Abstraction from OlefinAlthough smaller olefin species are always im-portant in virtually all combustion environ-ments,larger olefin species are generally much less important.At high temperatures,alkyl rad-icals decompose rapidly to smaller olefins,while at lower temperatures the RO˙2reaction paths, which produce only small quantities of large olefins,tend to dominate.As a result,we have chosen not to include a great deal of detail in the reactions of the larger olefins.We have assumed that,for olefins larger than C4,each alkene can have H atoms abstracted by H˙,O˙,O˙H,and C˙H3.However,because(1) site-specific abstraction rate constants from ole-fins are more difficult to estimate than from paraffins,(2)the number and complexity of the product species become difficult to follow,(3) the fate of those products are not very well understood,and(4)the sensitivity of the com-puted results to variations in these steps is very small,we decided to assume a single rate ex-pression for reactions of all of these large olefins with each of the radical species.An approximate rate value is assumed for each abstracting radical,intended to provide a rate constant averaged over primary,secondary,al-lylic,and vinylic C™H sites.As olefins get larger in carbon number,the double bond affects only a small portion of the molecule,the rest of which remains paraffinic in character.Thus,for large olefins,we expect the rate constants for H-atom abstraction to look more like those forTABLE2Rate Constant Expressions for C7Alkyl RadicalIsomerization Reactions.(cm3-mol-s-cal Units) IsomerizationRingSizeRate ExpressionᏭnᏱa 1C7H15º2C7H153 5.48ϫ108 1.6238,760 reverse 1.74ϫ107 2.0141,280 1C7H15º3C7H154 1.39ϫ1090.9833,760 reverse 4.41ϫ107 1.3836,280 1C7H15º3C7H156 4.28ϫ1011Ϫ1.0511,760 reverse 1.36ϫ1010Ϫ0.6614,280 1C7H15º4C7H155 2.54ϫ1090.3519,760 reverse 1.61ϫ1080.7422,280 2C7H15º3C7H1539.59ϫ108 1.3939,700 reverse9.59ϫ108 1.3939,700 2C7H15º3C7H155 3.22ϫ1090.1320,700 reverse 3.22ϫ1090.1320,700 2C7H15º4C7H154 1.76ϫ1090.7634,700 reverse 3.50ϫ1090.7634,700 3C7H15º4C7H153 6.04ϫ108 1.3939,700 reverse 1.20ϫ109 1.3939,700155MODELING STUDY OF n-HEPTANE OXIDATIONalkanes than smaller olefins.Furthermore,only one radical is assumed to be produced from each large olefin,which we designate as an “alkenyl”radical,an“average”of the possible vinylic,allylic,primary,and secondary radical species formed from n-heptane fuel.This type of treatment is expedient in the current study,and modifications would be needed in cases of specific interest.For exam-ple,if the study considered a large olefin as the primary fuel,this approach might be inappro-priate and refinements would be necessary.The rate constant expressions estimated in this mechanism are reported in Table3.Reaction Type7:Addition of Radical Species to OlefinSimilar to the discussion of type6reactions,we have tried to account for the possible addition reactions of small radicals with large olefin species.We have already considered the addi-tion reactions of H˙and C˙H3to olefins,as part of type3,-scission reactions above.HO˙2addition to olefins is considered later as the reverse of reaction type20.RO˙2addition to olefins was not considered.O˙H and O˙addition occurs at the double bond,which occupies only a small portion of a large olefin.Thus,theᏭ-factors below have been reduced to reflect the steric factor associ-ated with the probability of an O˙H or O˙radical reacting with the olefinic rather than the paraf-finic part of the molecule.The addition of O˙H or O˙radical to a large olefin forms an adduct not specifically included in the reaction mechanism.Instead,we have examined the likely-scission products of the adduct and used those as products for reaction type7.We will add more detail to the treatment of O˙H and O˙radical addition reactions as chemical understanding increases and computa-tional capabilities grow.We have found this procedure to be sufficient over the range of conditions applicable to this study.The specific reaction rate constants we have assumed are reported in Table4.Reaction Type8:Alkenyl Radical DecompositionSince H-atom abstraction from an olefin has been greatly simplified,we have also chosen to simplify the subsequent consumption of the average alkenyl radical formed to consist only of unimolecular decomposition to products we se-lected as being“reasonable”for the fuel.Thus in n-heptane,alkenyl radical decomposition is assumed to lead to products of allyl radicals and olefins.The rate constants of these decomposi-TABLE3Rate Constant Expressions for H-Atom Abstraction from C7Olefin(cm3-mol-s-cal Units)ReactionRate ExpressionReference ᏭnᏱaOlefinϩH˙ϭAlkenylϩH2 1.00ϫ10120.003,90066 OlefinϩO˙HϭAlkenylϩH2O 1.00ϫ10120.001,23066 OlefinϩO˙ϭAlkenylϩO˙H 1.00ϫ10120.004,00066 OlefinϩC˙H3ϭAlkenylϩCH4 2.00ϫ10110.007,30066TABLE4Rate Constant Expressions for O˙H and O˙Radical Addition to C7Olefin(cm3-mol-s-cal Units)ReactionRate ExpressionReference ᏭnᏱaOlefinϩO˙ϭProducts 2.00ϫ10100.001,05066 OlefinϩO˙HϭProducts 2.00ϫ10100.004,00066 156H.J.CURRAN ET AL.。
食品工艺学课件
⾷品⼯艺学课件Processing of fruitsInstructor: mingfeng zheng(郑明锋) phd.Email:vanheng@/doc/bca037d13186bceb19e8bb68.htmlCell: 138********注意:课件全部根据⽼师提供的ppt整理,在编号上可能会有些问题,所以⼤家将就着看,祝⼤家考试顺利。
Chapter one:introductionFruit quality and preprocessingObjectsThrough the introduction, the students knowThe relationship between quality of fruit and the processed product,The relationship between composition of fruit and the processed product,Quality attributes of fresh fruits, and quality measurementspreprocessing methods and technologies1.1 classification of fruitsFruits are commonly classified by growing region as follows. Temperate zone, subtropical, and tropical. Growing region and environmental conditions specific to each regionsignificantly affect fruit quality. Examples of fruit grown in each region are listed below:1) temperate zone fruits2) subtropical fruits3) tropical fruits(1) temperate zone fruitsPome fruits(仁果类): apple, asian pear (nashi), european pear, quince榅桲果Stone fruits: apricot杏, cherry, nectarine, peach, plumSmall fruits and berries: grape (european and american types), strawberry, raspberry, blueberry, blackberry, cranberry (2) subtropical fruitsCitrus fruits: grapefruit, lemon, lime, orange, pummelo, tangerine, and mandarinNoncitrus fruits: avocado, cherimaya, fig, kiwifruit, olive, pomegranate(3) tropical fruitsMajor tropical fruits: banana, mango, papaya, pineappleMinor tropical fruits: carambola, cashew apple, durian, guava,longan, lychee, mangosteen, passion fruit, rambutan1.2 quality of raw materialsThe quality of processed fruit products depends on their quality at the start of processing; How maturity at harvest, Harvesting methods,Post harvest handling proceduresMaintenance in fresh fruits between harvest and process initiation.Quality attributes of fresh fruitsAppearance、exture factors、flavor components、nutritional quality、safety factorsAppearance factorsSize、shape、color、freedom from defects and decay.Texture factorsFirmness, crispness, juiciness.Flavor componentsSweetness, sourness (acidity), astringency, (收敛),bitterness, aroma, off-flavors,Nutritional qualityFruit's content of vitamins (a and c are the most important in fruits), minerals, dietary fiber, carbohydrates, proteins. Safety factorsResidues of pesticides, presence of heavy metals, mycotoxins produced by certain species of fungi, microbial contamination.1.3 losses in fresh fruits after harvastWater loss,Physical injuries,physiological breakdown, decayLoss of acidity, flavor, color, and nutritive valueFactors influence fruit qualityIn the orchard,During transportation,Throughout the handling system (sorting, sizing, ripening, and storage).The total time between harvesting and processingMinimizing the delays throughout the post harvest handling system greatly reduces finality loss, especially in highly perishable fruits such as strawberries, blackberries, apricots, and cherries.1.4 contribution of fruits to human nutritionEnergy (calories)VitaminsMineralsDietary fiberThe us. Department of agriculture and other organizations currently encourage consumers to participate in the "five a day" program which focuses on consumption of five servings of either fruit or vegetables each day.Energy (calories)(1) carbohydrates: banana, breadfruit, raisin葡萄⼲(2) proteins & amino acids: nuts, dried apricot and fig(3) fats. Avocado, olive, nutsFruits typically contain between 10% and 25% carbohydrates, a small amount (less than1.0%) of proteins, and a very small amount (less than 0.5 %) of fat. Carbohydrates, sugars,and starches are broken down to co2, water, and energy during metabolism. Carbohydrates and fats provide most of the calories the body requires for heat and energy.Vitamins(1) fresh fruits and vegetables contribute about 91% of vitamin c, 48% of vitamin a, 27% of vitamin b6, 17% of thiamin硫胺(维⽣素b1) to diet.(2) the following fruits are important contributors (based on their vitamin content and the amount consumed) to the supply of indicated vitamins in the u.s. Diet:*vitamin a: apricot, peach, cherry, orange, watermelon, cantaloupe*vitamin c: strawberry, orange, grapefruit, banana, apple, cantaloupe* niacin烟酸: peach, banana, orange, apricot"*riboflavin核黄素: banana, peach, orange, apple* thiamin: orange, banana, grapefruit, appleMinerals(1) fresh fruits and vegetables contribute about 26% of the magnesium镁and 19% of the iron to the u.s. Diet.(2) the following fruits are important contributors to the supply of indicated minerals in the us. Diet:* potassium钾: banana, peach, orange, apple* phosphorus磷: banana, orange, peach, raisin, fig*calcium: tangerine, grapefruit, orange* iron: strawberry, banana, apple, orangeDietary fiber(1) all fruits and nuts contribute to the dietary fiber in the diet. Dietary fiber consists of cellulose, hemicellulose, lignin⽊质素, and pectic substances, which are derived primarily from fruit cell walls and skin.(2) the dietary fiber content of fruits ranges from 0.5-1.5% (fresh weight basis).(3) dietary fiber plays an important role in relieving constipation by increasing water-holding capacity of feces. Its consumption is also linked to decreased incidence of cardiovascular disease, diverticulosis, and colon cancer.factors influefncing composition and quality of fruitsPreharvest factors(1) genetic: selection of cultivars, differences in raw fruit composition, durability, and response to processing. Fruit cultivars grown for fresh market sale will not be the optimal cultivars for processing.(2) climatic: temperature, light, wind--climatic factors may have a strong influence on nutritional quality of fruits. Light intensity significantly affects vitamin concentration, and temperature influences transpiration rate, which will affect mineral uptake and metabolism. ?(3) cultural practices: soil type, soil nutrient and water supply, pruning修剪, thinning, pest control-fertilizer addition may significantly affect the mineral content of fruit.1. 5 maturity at harvest and harvesting methodMaturity at harvest is one of the primary factors affecting fruit composition, quality, and storage life. Although most fruits reach peak eating quality when harvested fully ripe, they are usually picked mature, but not ripe, to decrease mechanical damage during postharvest handling. Harvesting may also mechanically damage fruit; therefore, choice of harvest methodshould allow for maintenance of quality.Postharvest factors1) environmental,2) handling methods,3) time period between harvesting and consumption(1) environmentalTemperature, relative humidity, atmospheric composition,(2) handling methodsPostharvest handling systems involve the channels through which harvested fruit reaches the processing facility or consumer. Handling methods should be chosen such that they maintain fruit quality and avoid delays.(3) time period between harvesting and consumptionDelays between harvesting and cooling or processing may result in direct losses (due to water loss and decay) and indirect losses (decrease in flavor and nutritional quality).Fruit maturity, ripening, and quality relationshipsMaturity at harvest is the most important factor that determines storage life and final fruit quality. Immature fruits are of inferior quality when ripened. Overripe fruits are likely to become soft and with insipid flavor soon after harvest. Fruits picked either too early or too late in the season are more susceptible to physiological disorders and have a shorter storage life than those picked at mid-season.Maturity and ripeningIn general, fruits become sweeter, more colorful, and softer as they mature.Some fruits are usually picked mature but unripe so that they can withstand the postharvest handling system when shipped long distances. Most currently used maturity indices are based on a compromise between those indices that would ensure the best eating quality to the consumer and those that provide the needed flexibility in transportation and marketing.Carbohydrates(碳⽔化合物)Carbohydrates : fresh fruits vary greatly in their carbohydrate content, with a general range being between 10% and 25%;. The texture, taste, and food value of a fresh fruit is related to its carbohydrate content. Sucrose, glucose, and fructose are the primary sugars found in fruits.Fructose is sweeter than sucrose, and sucrose is sweeter than glucose.Starch is converted to sugar as the fruits mature and ripen.Proteins(蛋⽩质)Fruits contain less than 1% protein (as opposed to 9-20% protein in nuts such as almond, and walnut). Changes in the level and activity of proteins resulting from permeability changes in cell membranes may be involved in chilling injury. Enzymes, which catalyze metabolic processes in fruits, are proteins that are important in the reactions involved in fruit ripening and senescence.Enzymes in fruits:(Organic acids(有机酸)Organic acids are important intermediate products of metabolism. The krebs (tca) cycle is the main channel for the oxidation of organic acids in living cells, and it provides the energy required for maintenance of cell integrity. Organic acids aremetabolized into manyconstituents, including amino acids, which are the building blocks of proteins.Citric acid、malic acid、tartaric acid、oxalic acidPigments(⾊素)Pigments undergo many changes during the maturation and ripening of fruits.(1) loss of chlorophyll (green color), which is influenced by ph changes, oxidative conditions, and chlorophyllase action(2) synthesis and/or revelation of carotenoids (yellow and orange colors)(3) development of anthocyanins (red, blue, and purple colors.Beta-carotene is a precursor to vitamin a. Carotenoids are very stable and remain intact in fruit tissues, even when extensive senescence has occurred.Phenolic compounds(酚类化合物)Total phenolic content is higher in immature fruits than in mature fruits and is the main substrate involved in enzymatic browning of cut, or otherwise damaged, fruit tissues when exposed to air.Enzymatic browning(酶促褐变)Enzymatic browning occurs due to the oxidation of phenolic compounds and is mediated, in the presence of o2, by the enzyme polyphenoloxidase (ppo). The initial product of oxidation is usually o-quinone, which is highly unstable and undergoes polymerization to yield brown pigments of higher molecular weight. Polyphenoloxidase catalyzes the following tworeactions:Volatiles(挥发性)Volatiles are responsible for the characteristic aroma of fruits. They are present in extremely small quantities (c <100µg/g fresh wt.).Volatile compounds are largely esters(酯), alcohols, acids, aldehydes(醛), an d ketones (low-molecular weight compounds).VitaminsThe water-soluble vitamins includeVitamin c,Thiamin硫胺(维⽣素b1),Riboflavin核黄素,Niacin烟酸, vitamin b6,Folacin叶酸, vitamin b12, biotin维⽣素h. Fat soluble vitamins include vitamins a, d, e, and k.Fat-soluble vitamins are less susceptible to postharvest losses.Vitamin cAscorbic acid is most sensitive to destruction when the commodity is subjected to adverse handling and storage conditions. Losses are enhanced by extended storage, highertemperatures, low relative humidity, physical damage, and chilling injury. Postharvest losses in vitamins a and b are usually much smaller than losses in vitamin c.1.7 biological factors involved in postharvest deterioration (变坏) of fruits ?Respiration (呼吸作⽤)Ethylene productionTranspiration (蒸腾作⽤)Physiological disordersPhysical damagePathological breakdownRespirationStored organic materials (carbohydrates, proteins, fats) are broken down into simple end products with a release of energy. Oxygen (o2) is used in this process, and carbon dioxide (co2) is produced.The loss of stored food reserves in the commodity during respiration hastens senescence as the reserves that provide energy to maintain the commodity's living status are exhausted. ?Food value (energy value) for the consumer is lost; it has reduced flavor quality, with sweetness especially being lost; and salable dry weight is lost (especially important for commodities destined for dehydration). The energy released as heat.Ethylene productionEthylene, the simplest of the organic compounds affecting the physiological processes of plants, is produced by all tissues of higher plants. As a plant hormone, ethylene regulates many aspects of growth development, and senescence and is physiologically active in traceamounts (less than 0.1 ppm).Transpiration or water lossWater loss is the main cause of deterioration because it results not only direct quantitative.Losses (loss of salable weight) hut also in loss of its appearance, loss of cripsness, andjuiciness), and nutritional quality.The dermal system (outer protective coverings) governs the regulation of water loss by the commodity.Physiological disorders(1) freezing injury :usually results in immediate collapse of the tissues and total loss.(2) chilling injury when fruits (mainly those of tropical and subtropical origin) are held at temperatures above their freezing point and below 5-15℃, depending on the commodity. ?(3) heat injury results from exposure to direct sunlight or to excessively high temperatures.Symptoms include surface scalding, uneven ripening, excessive softening, and desiccation. ?(4) very low (<1%) oxygen and/or elevated (>20%) carbon dioxide concentration can result in physiological breakdown of all fruits.Physical damageVarious types of physical damage (surface injuries, impact bruising, vibration bruising, etc.) Are major contributors to deterioration. Mechanical injuries are not only unsightly, but also accelerate water loss, stimulate higher respiration and ethylene production rates, and favor decay incidence.Pathological breakdownDecay is one of the most common or apparent causes of deterioration; however, attack by many microorganisms usually follows mechanical injury or physiological breakdown, which allows entry to the microorganism. Pathogens can infect healthy tissues and become the primary cause of deterioration.Environmental factors influencing deterioration of fruits(影响⽔果变坏的环境因素)Temperature,Relative humidity,Air movement,Atmospheric composition,Ethylene,Harvesting procedures Postharvest handling proceduresDumping、Sorting、Sizing、Cooling、Storage、RipeningDumping:Fresh fruits should be handled with care throughout the postharvest handling system in order to minimize mechanical injuries. Dumping in water or in flotation tanks should be used for fruits. If dry dumping systems are used, they should be well padded bruising. Sorting:Manual sorting is usually carried out to eliminate fruit exhibiting defects or decay. For some fruits, it may also be necessary to sort the fruit into two or more classes of maturity or ripeness.Mechanical sorters, which operate on the basis of color, soluble solids, moisture, or fat content, are being implemented and may greatly reduce time and labor requirements. Sizing:In some cases, sizing the fruits into two or more size categories may be required before processing. Sizing can be done mechanically on the basis of fruit dimension or by weight.Mechanical sizing can be a major source of physical damage to the fruit if the machines are not adequately padded and adjusted to the minimum possible fruit drop heights Ripening:Ripening before processing may be required for certain fruits (banana, kiwifruit, mango, papaya, peach, pear, plum, melon) that are picked mature but unripe. Ethylene treatment can be used to obtain faster and more uniform ripening. The optimum temperature range for ripening is 15-25℃and, within this range, the higher the temperature, the faster the ripening. Relative humidity should be maintained between 90% and 95 % during ripening. Cooling:Cooling is utilized to remove field heat and lower the fresh fruit's temperature to near its optimum storage temperature. Cooling can be done using cold water (hydrocooling) or cold air (forced-air cooling or "pressure cooling"). Highly perishable fruits, such as strawberries, bush berries, and apricots, should be cooled to near 4℃within six hours of harvest. Other fruits should be cooled to their optimum temperature within twelve hours of harvest. Storage:Short-term or long-term storage of fresh fruits may be needed before processing to regulate the product flow and extend the processing season. The relative humidity in the storage facility should be kept between 90% and 95%.To reduce decay, elevated c02 (15-20%) may be added to the atmosphere within pallet covers for strawberries, bush berries, and cherries, and sulfur dioxide (200 ppm) fumigation may be used on grapes.1.8 quality measurementsMany quality measurements can be made before a fruit crop is picked in order to determine if proper maturity or degree of ripeness has developed.ColourColour may be measured with instruments or by comparing the colour of fruit on the tree with standard picture charts. TextureTexture may be measured by compression by hand or by simple type of plungers.Soluble solidsAs fruit mature on the tree its concentration of juice solids, which are mostly sugars, changes. The concentration of soluble solids in the juice can be estimated with arefractometer or a hydrometer液体⽐重计.Acid contentThe acid content of fruit changes with maturity and affects flavour. Acid concentration can be measured by a simple chemical titration on the fruit juice. But for many fruits the tartness and flavour are really affected by the ratio of sugar to acid. Sugar to acid ratioIn describing the taste of tartness of several fruits and fruit juices, the term "sugar to acid ratio" or "brix to acid ratio" are commonly used. The higher the brix the greater the sugar concentration in the juice; the higher the "brix to acid ratio" the sweeter and lees tart is the juice.1.9 preprocessing1.9.1 harvestingThe above and other measurements, plus experience, indicate when fruit is ready for harvesting and subsequent processing.1.9.2 reception - quality and quantity1.9.3 temporary storage before processing1.9.4 washingHarvested fruit is washed to remove soil, micro-organisms and pesticide residues.Fruit washing is a mandatory processing step; it would be wise to eliminate spoiled fruit before washing in order to avoid the pollution of washing tools and/or equipment and the contamination of fruit during washing.1.9.5 sortingFruit sorting covers two main separate processing operations:Removal of damaged fruit and any foreign bodies (which might have been left behind after washing);Qualitative sorting based on organoleptic criteria and maturity stage.Mechanical sorting for size is usually not done at the preliminary stage. The most important initial sorting is for variety and maturity.1.9.6 trimming and peeling (skin removal)This processing step aims at removing the parts of the fruit which are either not edible or difficult to digest especially the skin.Up to now the industrial peeling of fruit and vegetables was performed by three procedures: Mechanically;By using water steam;Chemically; this method consists in treating fruit and vegetables by dipping them in a caustic soda solution at a temperature of 90 to 100°c; the concentration of this solution as well asthe dipping or immersion time varying according to each specific case.1.9.7 cuttingThis step is performed according to the specific requirements of the fruit processing technology.1.9.8 blanchingA brief heat treatment to vegetables some fruits to inactivate oxidative enzyme systems such as catalase, peroxidase, polyphenoloxidase, ascorbic acid oxidase, and lipoxygenase. ?When the unblanched tissue is disrupted or bruised and exposed to air, these enzymes come in contact with substrates causing softening, discoloration, and the production of off flavors. ?It is most often standard practice to blanch fruits in order to prevent quality deterioration. ?Although the primary purpose of blanching is enzyme inactivation.There are several other benefits blanching initially cleanses the product;Decreases the microbial load,Preheats the product before processing.Softens the fruit, facilitates compact packing in the can.Expell intercellular gases in the raw fruitImproved heat transfer during heat processing.Water blanching is generally of the immersion type or spray type as the product moves on a conveyor.Steam blanching often involves belt or chain conveyors upon which the product moves through a tunnel containing live steam.adequacy of blanching is usually based on inactivation of one of the heat resistant enzymes (peroxidase or polyphenol oxidase).During the blanching process, it is imperative that certain enzymes that have the potential to cause flavour and textural changes be inactiviated. The process involves a brief heattreatment applied to most vegetables and also to some fruits in order to inactivate oxidative enzyme system such as catalase, peroxidase, polyphenoloxidase,ascorbic acid oxidase, and lipoxygenase.When unblanched tissue is disrupted or bruised and exposed to air,these enzymes come in contact with substrate causing softening,discoloration, and the production of off-flavours.Since this action can potentially occur during the period prior to heat processing, it is most often standard practice to blanch fruits in order to prevent quality deterioration.1.9.9 ascorbic/citric acid dipAscorbic acid or vitamin c minimises fruit oxidation primarily by acting as an antioxidant and itself becoming oxidised in preference to catechol⼉茶酚-tannin compounds.It has been found that increased acidity also helps retard oxidative colour changes and so ascorbic acid plus citric acid may be used together. Citric acid further reacts with (chelates) metal ions thus removing these catalysts of oxidation from the system.1.9.10 sulphur dioxide treatmentSulphur dioxide may function in several ways:Sulphur dioxide is an enzyme poison against common oxidising enzymes;It also has antioxidant properties; i.e., it is an oxygen acceptor (as is ascorbic acid);Further so2 minimises non enzymatic maillard type browning by reacting with aldehyde醛groups of sugars so that they are no longer free to combine with amino acids;Sulphur dioxide also interferes with microbial growth.In many fruit processing pre-treatments two factors must be considered:Sulphur dioxide must be given time to penetrate the fruit tissues;So2 must not be used in excess because it has a characteristic unpleasant taste and odour, and international food laws limit the so2 content of fruit products, especially of those which are consumer oriented (e.g. Except semi-processed products oriented to further industrial utilisation).5.2.11 sugar syrupSugar syrup addition is one of the oldest methods of minimising oxidation.Sugar syrup minimises oxidation by coating the fruit and thereby preventing contact withatmospheric oxygen.Sugar syrup also offers some protection against loss of volatile 挥发性的fruit esters 酯and itcontributes sweet taste to otherwise tart fruits.It is common today to dissolve ascorbic acid and citric acid in the sugar syrup for addedeffect or to include sugar syrup after an so 2 treatment.QuestionsWhat factors influence the quality of fruits after harvest?How to maintain the fruit in good quality before the processing begin?第⼀节果蔬原料特性新鲜果蔬原料的特点 ? 果蔬原料的化学成分原料的化学成分与加⼯的关系1.新鲜果蔬原料的特点易腐性、季节性、区域性2.果蔬中的化学成分(chemical composition in fruits and vegetables )3.化学成分与加⼯的关系(relation between chemical composition and processing )3.1 ⽔分(water)果蔬中⽔的含量:⼤多数在80%以上,含⽔量⾼的如冬⽠(wax gourd)可达96%以上。
pisa2006科学试题
Document: ReleasedPISAItems_Science.docPISA RELEASED ITEMS - SCIENCEDecember 2006Table of ContentsS126: Biodiversity (3)S127: Buses (6)S128: Cloning (8)S129: Daylight (11)S195: Semmelweis’ Diary (16)S210: Climate Change (22)S212: Flies (24)S251: Calf Clones (28)S253: Ozone (31)S307: Corn (37)S409: Fit for Drinking (40)S414: Tooth Decay (45)S420: Hot Work (48)S423: Mousepox (50)S433: Stickleback Behaviour (53)S439: Tobacco Smoking (59)S441: Starlight (63)S448: Ultrasound (64)S470: Lip Gloss (67)S472: Evolution (69)S505: Bread Dough (72)S507: Transit of Venus (76)S515: Health Risk? (79)S516: Catalytic Converter (82)S526: Major Surgery (86)S529: Wind Farms (90)Source Publications for Released Items (94)S126: BiodiversityBiodiversity Text 1Read the following newspaper article and answer the questions which follow.BIODIVERSITY IS THE KEY TO MANAGING ENVIRONMENTAn ecosystem that retains a high biodiversity (that is, a wide variety of living things) is much more likely to adapt to human-caused environment change than is one that has little. Consider the two food webs shown in the diagram. The arrows point from the organism that gets eaten to the one that eats it. These food webs are highly5simplified compared with food webs in real ecosystems, but they still illustrate a key difference between more diverse and less diverse ecosystems. Food web B represents a situation with very low biodiversity, where at some levels the food path involves only a single type of organism. Food web A represents a more diverse ecosystem with, as a result, many more alternative feeding pathways. 10Generally, loss of biodiversity should be regarded seriously, not only because the organisms that have become extinct represent a big loss for both ethical and utilitarian (useful benefit) reasons, but also because the organisms that remain have become more vulnerable (exposed) to extinction in the future.Source: Adapted from Steve Malcolm: ‘Biodiversity is the key to managing environment’, The Age , 16 August 1994.FOOD WEB A FOOD WEB BEucalypt Beetle Spider Lizard SnakeWattle Tea TreeLeaf HopperButterfly LarvaeParasitic Wasp HoneyeaterRobinButcher BirdNative CatNative CatButcher BirdSnakeLizard RobinParasitic WaspLeaf HopperWattleQuestion 3: BIODIVERSITY S126Q03 In lines 9 and 10 it is stated that “Food web A represents a more diverse ecosystem with, as a result, many more alternative feeding pathways.”Look at FOOD WEB A. Only two animals in this food web have three direct (immediate) food sources. Which two animals are they?A Native Cat and Parasitic WaspB Native Cat and Butcher BirdC Parasitic Wasp and Leaf HopperD Parasitic Wasp and SpiderE Native Cat and HoneyeaterBIODIVERSITY SCORING 3QUESTION INTENT: Process: Demonstrating knowledge and understandingTheme: EcosystemsArea: Science in life and healthFull creditCode 1: A. Native Cat and Parasitic WaspNo creditCode 0: Other responses.Code 9: Missing.Question 4: BIODIVERSITY S126Q04 Food webs A and B are in different locations. Imagine if Leaf Hoppers died out in both locations. Which one of these is the best prediction and explanation for the effect this would have on the food webs?A The effect would be greater in food web A because the Parasitic Wasp has onlyone food source in web A.B The effect would be greater in food web A because the Parasitic Wasp hasseveral food sources in web A.C The effect would be greater in food web B because the Parasitic Wasp has onlyone food source in web B.D The effect would be greater in food web B because the Parasitic Wasp hasseveral food sources in web B.BIODIVERSITY SCORING 4QUESTION INTENT: Process: Drawing/evaluating conclusionsTheme: BiodiversityArea: Science in life and healthFull creditCode 1: C. The effect would be greater in food web B because the Parasitic Wasp has only one food source in web B.No creditCode 0: Other responses.Code 9: Missing.S127: BusesQuestion 1: BUSES S127Q01 A bus is driving along a straight stretch of road. The bus driver, named Ray, has a cup of water resting on the dashboard:1 2waterdriving directionSuddenly Ray has to slam on the brakes.What is most likely to happen to the water in the cup?A The water will stay horizontal.B The water will spill over side 1.C The water will spill over side 2.D The water will spill but you cannot tell if it will spill at side 1 or side 2.BUSES SCORING 1QUESTION INTENT: Process: Demonstrating knowledge and understandingTheme: Forces and movementArea: Science in technologiesFull creditCode 1: C. The water will spill over side 2.No creditCode 0: Other responses.Code 9: Missing.Question 4: BUSES S127Q04-0189 Ray’s bus is, like most buses, powered by a petrol engine. These buses contribute to environmental pollution.Some cities have trolley buses: they are powered by an electric engine. The voltage needed for such an electric engine is provided by overhead lines (like electric trains). The electricity is supplied by a power station using fossil fuels.Supporters for the use of trolley buses in a city say that these buses don’t contribute to environmental pollution.Are these supporters right? Explain your answer. .................................................... ................................................................................................................................... ................................................................................................................................... ...................................................................................................................................BUSES SCORING 4QUESTION INTENT: Process: Demonstrating knowledge and understandingTheme: Energy transformationsArea: Science in Earth and environmentFull creditCode1: Gives an answer in which it is stated that the power station also contributes to environmental pollution:• No, because the power station causes environmental pollution as well.• Yes, but this is only true for the city itself; the power station however causesenvironmental pollution.No creditCode 0: No or yes, without a correct explanation.Code 8: Off task.Code 9: Missing.Example responsesCode 1:• Yes and No. The buses don’t pollute the city which is good, but the power stationdoes pollute and that’s not very good.• The buses do contribute to the environmental pollution by using fossil fuels butthey’re not as harmful as normal buses with all their gases. [Note: This answercan be given the benefit of the doubt.]Code 0:• Well they have no outlet so no harmful smoke goes into the air which candamage the O-zone layer, and having electricity created by fossil fuels is alsomore environmental friendly.• Yes, they are. Because electricity isn’t harmful for the environment we only useup our Earth’s gas.S128: CloningRead the newspaper article and answer the questions that follow.Question 1: CLONING S128Q01 Which sheep is Dolly identical to?A Sheep 1B Sheep 2C Sheep 3D Dolly’s fatherCLONING SCORING 1Full creditCode 1: A. Sheep 1No creditCode 0: Other responses.Code 9: Missing.Question 2: CLONING S128Q02 In line 14 the part of the udder that was used is described as “a very small piece”. From the article text you can work out what is meant by “a very small piece”.That “very small piece” isA a cell.B a gene.C a cell nucleus.D a chromosome.CLONING SCORING 2Full creditCode 1: A. a cell.No creditCode 0: Other responses.Code 9: Missing.Question 3: CLONING S128Q03In the last sentence of the article it is stated that many governments have alreadydecided to forbid cloning of people by law.Two possible reasons for this decision are mentioned below.Are these reasons scientific reasons?Circle either “Yes” or “No” for each.Reason: Scientific? Cloned people could be more sensitive to certain diseases thanYes / Nonormal people.People should not take over the role of a Creator. Yes / NoCLONING SCORING 3Full creditCode 1: Yes, No, in that order.No creditCode 0: Other responses.Code 9: Missing.S129: DaylightRead the following information and answer the questions that follow. DAYLIGHT ON 22 JUNE 2002Today, as the Northern Hemisphere celebrates its longest day, Australians will experience their shortest.In Melbourne*, Australia, the Sun will rise at 7:36 am and set at 5:08 pm, giving nine hours and 32 minutes of daylight. Compare today to the year’s longest day in the Southern Hemisphere, expected on 22 December, when the Sun will rise at 5:55 am and set at 8:42 pm, giving 14 hours and 47 minutes of daylight.The President of the Astronomical Society, Mr Perry Vlahos, said the existence of changing seasons in the Northern and Southern Hemispheres was linked to the Earth’s 23-degree tilt.*Melbourne is a city in Australia at a latitude of about 38 degrees South of the equator.Question 1: DAYLIGHT S129Q01 Which statement explains why daylight and darkness occur on Earth?A The Earth rotates on its axis.B The Sun rotates on its axis.C The Earth’s axis is tilted.D The Earth revolves around the Sun.DAYLIGHT SCORING 1Full creditCode 1: A. The Earth rotates on its axis.No creditCode 0: Other responses.Code 9: Missing.Question 2: DAYLIGHT S129Q02 - 01 02 03 04 11 12 13 21 99 In the Figure light rays from the Sun are shown shining on the Earth.Suppose it is the shortest day in Melbourne.Show the Earth’s axis, the Northern Hemisphere, the Southern Hemisphere and the Equator on the Figure. Label all parts of your answer.DAYLIGHT SCORING 2Note: the important features when marking this question are:1. The Earth’s axis is drawn tilted towards the Sun within the range 10° and 45° from vertical for credit: refer to the following diagram:Outside of 10° and 45° to vertical range: no credit.2. The presence or absence of clearly labelled Northern and Southern Hemispheres, or one Hemisphere only labelled, the other implied.3. The equator is drawn at a tilt towards the Sun within the range 10° and 45° above horizontal for credit: refer to the following diagram:CREDIT FOR AXIS10O 23O 45OFigure: light rays from SunThe equator may be drawn as an elliptical line or straight line.Outside of 10° and 45° to horizontal range: no credit.Full creditCode 21: Diagram with Equator tilted towards the Sun at an angle between 10° and45° and Earth’s axis tilted towards the Sun within the range 10° and 45°from vertical, and the Northern and or Southern Hemispheres correctlylabelled (or one only labelled, the other implied).Partial creditCode 11: Angle of tilt of axis between 10° and 45°, Northern and / or SouthernHemispheres correctly labelled (or one only labelled, the other implied), butangle of tilt of Equator not between 10° and 45°; or Equator missing.CREDIT FOR EQUATOR 10O23O45ONS A EquatorAxis N Equator Axis N NAxisS S EquatorCode 12: Angle of tilt of Equator between 10° and 45°, Northern and / or SouthernHemispheres correctly labelled (or one only labelled, the other implied), butangle of tilt of axis not between 10° and 45°; or axis missing.Code 13: Angle of tilt of Equator between 10° and 45°, and angle of tilt of axisbetween 10° and 45°, but Northern and Southern Hemispheres notcorrectly labelled (or one only labelled, the other implied, or both missing).No creditCode 01: Northern and or Southern Hemispheres correctly labelled (or one only, theother implied) is the only correct feature.Code 02: Angle of tilt of Equator between 10° and 45° is the only correct feature.NS AxisEquator N S Axis Equator N Axis Equator Axis EquatorN SEquatorCode 03: Angle of tilt of axis between 10° and 45° is the only correct feature.AxisCode 04: No features are correct, or other responses.SNCode 99: Missing.S195: Semmelweis’ DiarySemmelweis’ Diary Text 1‘July 1846. Next week I will take up a position as “Herr Doktor” at the First Ward of the maternity clinic of the Vienna General Hospital. I was frightened when I heard about the percentage of patients who die in this clinic. This month not less than 36 of the 208 mothers died there, all from puerperal fever. Giving birth to a child is as dangerous as first-degree pneumonia.’These lines from the diary ofIgnaz Semmelweis (1818-1865)illustrate the devastating effects of puerperal fever, a contagious disease that killed many women after childbirth. Semmelweiscollected data about the numberof deaths from puerperal fever in both the First and the SecondWards (see diagram).Physicians, among them Semmelweis, were completely in the dark about the cause of puerperal fever. Semmelweis’ diary again:‘December 1846. Why do so many women die from this fever after giving birth without any problems? For centuries science has told us that it is an invisible epidemic that kills mothers. Causes may be changes in the air or some extraterrestrial influence or a movement of the earth itself, an earthquake.’Nowadays not many people would consider extraterrestrial influence or anearthquake as possible causes of fever. But in the time Semmelweis lived, many people, even scientists, did! We now know it has to do with hygienic conditions. Semmelweis knew that it was unlikely that fever could be caused by extraterrestrial influence or an earthquake. He pointed at the data he collected (see diagram) and used this to try to persuade his colleagues.Diagram184118421843184418451846Year15105Number of Deaths First WardSecondWardNumber of Deaths per 100 deliveries from puerperal feverQuestion 2: SEMMELWEIS’ DIARY S195Q02- 01 02 03 04 11 12 13 21 99 Suppose you were Semmelweis. Give a reason (based on the data Semmelweis collected) why puerperal fever is unlikely to be caused by earthquakes. ................................................................................................................................... ................................................................................................................................... ................................................................................................................................... ...................................................................................................................................SEMMELWEIS’ DIARY SCORING 2QUESTION INTENT: Process: Drawing/evaluating conclusionsTheme: Human biologyArea: Science in life and healthFull creditCode 21: Refers to the difference between the number of deaths (per 100 deliveries) in both wards.• Due to the fact that the first ward had a high rate of women dying compared towomen in the second ward, obviously shows that it had nothing to do withearthquakes.• Not as many people died in ward 2 so an earthquake couldn’t have occurredwithout causing the same number of deaths in each ward.•Because the second ward isn’t as high, maybe it had something to do with ward 1.• It is unlikely that earthquakes cause the fever since death rates are so differentfor the two wards.Partial creditCode 11: Refers to the fact that earthquakes don’t occur frequently.• It would be unlikely to be caused by earthquakes because earthquakes wouldn’thappen all the time.Code 12: Refers to the fact that earthquakes also influence people outside the wards.• If there were an earthquake, women from outside the hospital would have gotpuerperal fever as well.• If an earthquake were the reason, the whole world would get puerperal fevereach time an earthquake occurs (not only the wards 1 and 2).Code 13: Refers to the thought that when earthquakes occur, men don’t get puerperal fever.• If a man were in the hospital and an earthquake came, he didn’t get puerperalfever, so earthquakes cannot be the cause.• Because girls get it and not men.No creditCode 01: States (only) that earthquakes cannot cause the fever.• An earthquake cannot influence a person or make him sick.• A little shaking cannot be dangerous.Code 02: States (only) that the fever must have another cause (right or wrong).• Earthquakes do not let out poison gases. They are caused by the plates of theEarth folding and faulting into each other.• Because they have nothing to do with each other and it is just superstition.• An earthquake doesn’t have any influence on the pregnancy. The reason wasthat the doctors were not specialised enough.Code 03: Answers that are combinations of Codes 01 and 02.• Puerperal fever is unlikely to be caused by earthquakes as many women dieafter giving birth without any problems. Science has told us that it is an invisibleepidemic that kills mothers.• The death is caused by bacteria and the earthquakes cannot influence them. Code 04: Other responses.• I think it was a big earthquake that shook a lot.• In 1843 the deaths decreased at ward 1 and less so at ward 2.• Because there aren’t any earthquakes by the wards and they still got it. [Note:The assumption that there were no earthquakes at that time isn’t correct.] Code 99: Missing.Semmelweis’ Diary Text 2Part of the research in the hospital was dissection. The body of a deceased person was cut open to find a cause of death. Semmelweis recorded that the students working on the First ward usually took part in dissections on women who died the previous day, before they examined women who had just given birth. They did not pay much attention to cleaning themselves after the dissections. Some were even proud of the fact that you could tell by their smell that they had been working in the mortuary, as this showed how industrious they were!One of Semmelweis’ friends died after having cut himself during such a dissection. Dissection of his body showed he had the same symptoms as mothers who died from puerperal fever. This gave Semmelweis a new idea.Question 4: SEMMELWEIS’ DIARY S195Q04 Semmelweis’ new idea had to do with the high percentage of women dying in the maternity wards and the students’ behaviour.What was this idea?A Having students clean themselves after dissections should lead to a decrease ofpuerperal fever.B Students should not take part in dissections because they may cut themselves.C Students smell because they do not clean themselves after a dissection.D Students want to show that they are industrious, which makes them carelesswhen they examine the women.SEMMELWEIS’ DIARY SCORING 4QUESTION INTENT: Process: Recognising questionsTheme: Human biologyArea: Science in life and healthFull creditCode 1: A. Having students clean themselves after dissections should lead to a decrease of puerperal fever.No creditCode 0: Other responses.Code 9: Missing.Question 5: SEMMELWEIS’ DIARY S195Q05-01 02 11 12 13 14 15 99 Semmelweis succeeded in his attempts to reduce the number of deaths due to puerperal fever. But puerperal fever even today remains a disease that is difficult to eliminate.Fevers that are difficult to cure are still a problem in hospitals. Many routine measures serve to control this problem. Among those measures are washing sheets at high temperatures.Explain why high temperature (while washing sheets) helps to reduce the risk that patients will contract a fever. ......................................................................................................................................................................................................................................................................SEMMELWEIS’ DIARY SCORING 5QUESTION INTENT: Process: Demonstrating knowledge and understandingTheme: Human biologyArea: Science in life and healthFull creditCode 11: Refers to killing of bacteria .• Because with the heat many bacteria will die.• Bacteria will not stand the high temperature.• Bacteria will be burnt by the high temperature.• Bacteria will be cooked. [Note: Although “burnt” and “cooked” are notscientifically correct, each of the last two answers as a whole can be regardedas correct.]Code 12: Refers to killing of microorganisms, germs or viruses.• Because high heat kills small organisms which cause disease.• It’s too hot for germs to live.Code 13: Refers to the removal (not killing) of bacteria.• The bacteria will be gone.• The number of bacteria will decrease.• You wash the bacteria away at high temperatures.Code 14: Refers to the removal (not killing) of microorganisms, germs or viruses.• Because you won’t have the germ on your body.Code 15: Refers to sterilisation of the sheets.• The sheets will be sterilised.No creditCode 01: Refers to killing of disease.• Because the hot water temperature kills any disease on the sheets.• The high temperature kills most of the fever on the sheets, leaving less chanceof contamination.Code 02: Other responses.• So they don’t get sick from the cold.• Well when you wash something it washes away the germs.Code 99: Missing.Question 6: SEMMELWEIS’ DIARY S195Q06 Many diseases may be cured by using antibiotics. However, the success of some antibiotics against puerperal fever has diminished in recent years.What is the reason for this?A Once produced, antibiotics gradually lose their activity.B Bacteria become resistant to antibiotics.C These antibiotics only help against puerperal fever, but not against otherdiseases.D The need for these antibiotics has been reduced because public health conditionshave improved considerably in recent years.SEMMELWEIS’ DIARY SCORING 6QUESTION INTENT: Process: Demonstrating knowledge and understandingTheme: BiodiversityArea: Science in life and healthFull creditCode 1: B. Bacteria become resistant to antibiotics.No creditCode 0: Other responses.Code 9: Missing.S210: Climate ChangeClimate Change Text 1Read the following information and answer the questions which follow.WHAT HUMAN ACTIVITIES CONTRIBUTE TO CLIMATE CHANGE?The burning of coal, oil and natural gas, as well as deforestation and variousagricultural and industrial practices, are altering the composition of the atmosphere and contributing to climate change. These human activities have led to increased concentrations of particles and greenhouse gases in the atmosphere. The relative importance of the main contributors to temperature change is shown in Figure 1. Increased concentrations of carbon dioxide and methane have a heating effect. Increased concentrations of particles have a cooling effect in two ways, labelled ‘Particles’ and ‘Particle effects on clouds’.Figure 1: Relative importance of the main contributors to change intemperature of the atmosphere.Bars extending to the right of the centre line indicate a heating effect. Bars extending to the left of the centre line indicate a cooling effect. The relative effect of ‘Particles’ and ‘Particle effects on clouds’ are quite uncertain: in each case the possible effect is somewhere in the range shown by the light grey bar.Source: adapted from /ipcc/qa/04.htmlCooling Relative ImportanceHeatingQuestion 1: CLIMATE CHANGE S210Q01-01289 Use the information in Figure 1 to develop an argument in support of reducing the emission of carbon dioxide from the human activities mentioned. ................................................................................................................................... ................................................................................................................................... ................................................................................................................................... CLIMATE CHANGE SCORING 1QUESTION INTENT: Process: CommunicatingTheme: The Earth and its place in the universeArea: Science in Earth and environmentFull creditCode 2: Carbon dioxide is the main factor causing an increase in atmospheric temperature/causing climatic change, so reducing the amount emitted willhave the greatest effect in reducing the impact of human activities.Partial creditCode 1: Carbon dioxide is causing an increase in atmospheric temperature/causing climatic change.No creditCode 0: Other responses, including that an increase in temperature will have a bad effect on the Earth.Code 8: Off task.Code 9: Missing.Example responsesCode 2:• The emission of CO2 causes significant heating to the atmosphere and thereforeshould be lessened. [Note: The term “significant” can be considered asequivalent to “most”. ]• According to figure 1 reduction in the emission of carbon dioxide is necessarybecause it considerably heats the earth. [Note: The term “considerable” can beconsidered as equivalent to “most”.]Code 1:• The burning of fossil fuel such as oil, gas and coal are contributing to the buildup of gases in the atmosphere, one of which is carbon dioxide (CO2). This gasaffects the temperature of the earth which increases causing a greenhouseeffect.Code 0:• The way that humans could help control carbon dioxide levels to drop would beby not driving a car, don’t burn coal and don’t chop down forests. [Note: Noconsideration given to the effect of carbon dioxide on temperature.]S212: FliesFlies Text 1Read the following information and answer the questions which follow.FLIESA farmer was working with dairy cattle at an agricultural experiment station. The population of flies in the barn where the cattle lived was so large that the animals’ health was affected. So the farmer sprayed the barn and the cattle with a solution of insecticide A. The insecticide killed nearly all the flies. Some time later, however,the number of flies was again large. The farmer again sprayed with the insecticide. The result was similar to that of the first spraying. Most, but not all, of the flies were killed. Again, within a short time the population of flies increased, and they were again sprayed with the insecticide. This sequence of events was repeated five times: then it became apparent that insecticide A was becoming less and less effective in killing the flies.The farmer noted that one large batch of the insecticide solution had been made and used in all the sprayings. Therefore he suggested the possibility that the insecticide solution decomposed with age.Source: Teaching About Evolution and the Nature of Science, National Academy Press, Washington, DC, 1998, p. 75.Question 1: FLIES S212Q01-01234589 The farmer’s suggestion is that the insecticide decomposed with age. Briefly explain how this suggestion could be tested. ................................................................................................................................... ................................................................................................................................... ...................................................................................................................................FLIES SCORING 1QUESTION INTENT: Process: Identifying evidenceTheme: Chemical and physical changesArea: Science in life and healthFull creditCode 5: Applies to answers in which three variables (type of flies, age ofinsecticide, and exposure) are controlled eg. Compare the results from anew batch of the insecticide with results from the old batch on two groupsof flies of the same species that have not been previously exposed to theinsecticide.。
维生素d结合反应元件的全英文单词
维生素d结合反应元件的全英文单词Vitamin D Binding Response Element (VDRE)Vitamin D is a crucial nutrient that plays a vital role in various physiological processes in the human body. It is essential for maintaining healthy bones, regulating the immune system, and supporting overall health and well-being. One of the key mechanisms through which vitamin D exerts its effects is by binding to specific genomic regions known as Vitamin D Response Elements (VDREs).VDREs are short DNA sequences within the promoter regions of target genes that are recognized by the vitamin D receptor (VDR). Upon binding to VDREs, VDR forms a complex with other transcription factors and coactivators, leading to the modulation of gene expression. This results in the activation or repression of target genes involved in processes such as calcium homeostasis, cell growth, and immune function.The functional unit of VDREs consists of a direct repeat of two hexameric sequences separated by a 3-base pair spacer. The consensus sequence for VDREs is 5’-RGKTSA-3’, where R represents a purine base (adenine or guanine), K represents a pyrimidine base (thymine or cytosine), T represents a thyminebase, S represents a guanine base, and A represents an adenine base.The binding of vitamin D to VDREs triggers a series of events that ultimately lead to changes in gene expression. This can result in the upregulation of genes involved in bone mineralization, immune response, and cell differentiation, among other processes. On the other hand, the binding of vitamin D to negative VDREs can lead to the suppression of genes involved in inflammation and cancer progression.In conclusion, Vitamin D Binding Response Elements (VDREs) play a critical role in mediating the effects of vitamin D on gene expression. By binding to VDREs, vitamin D can regulate the expression of key genes involved in various physiological processes, thereby contributing to overall health and well-being. Further research into the mechanisms underlying VDRE function may provide valuable insights into the therapeutic potential of vitamin D in the prevention and treatment of various diseases.。
写一篇感谢辅酶的英语作文
写一篇感谢辅酶的英语作文英文回答:Enzymes, the unsung heroes of life, play an indispensable role in the intricate dance of cellular processes. These macromolecular catalysts orchestrate an astonishing array of chemical reactions, poweringeverything from metabolism to DNA replication. From the simplest of organisms to the most complex human bodies, enzymes are the tireless workers behind the scenes,enabling life as we know it.The diversity of enzymes is as vast as the kingdom oflife itself. Each enzyme is meticulously designed for a specific task, with its unique shape and composition conferring unparalleled substrate specificity. Some enzymes, like proteases, facilitate the breakdown of proteins, while others, such as kinases, catalyze the transfer of phosphate groups. Together, this ensemble of enzymatic playersensures the smooth operation of cellular machinery.The importance of enzymes cannot be overstated. Without them, biochemical reactions would proceed at an excruciatingly slow pace, rendering life impossible. Enzymes accelerate these reactions by lowering the activation energy required for their initiation. This remarkable ability enables biological processes to occur at physiological temperatures, preventing the thermal destruction of cellular components.Moreover, enzymes demonstrate remarkable selectivity, ensuring that specific reactions occur with precision. This selectivity stems from the precise fit between the enzyme's active site and its substrate, a phenomenon known as the lock-and-key model. This molecular choreography ensuresthat only the intended substrates are targeted, preventing unwanted side reactions.The human body relies heavily on enzymes to maintain homeostasis and carry out essential functions. Digestive enzymes, such as amylase and pepsin, break down food into digestible nutrients. In the respiratory chain, oxidativeenzymes harness the energy stored in glucose, fueling cellular activities. Hormones, such as insulin, facilitate glucose uptake by binding to specific receptors on cell membranes. These are but a few examples of the countless enzymatic processes that sustain life.The field of enzymology has witnessed tremendous advancements in recent decades. The development of techniques such as X-ray crystallography and molecular dynamics simulations has provided unprecedented insights into the structure and function of enzymes. This knowledge has fueled the development of enzyme inhibitors, which are used to treat a wide range of diseases. For instance, ACE inhibitors are used to manage hypertension, and statins inhibit cholesterol synthesis, reducing the risk of cardiovascular disease.Enzyme technology has also emerged as a powerful tool for industrial applications. Enzymes are used in various sectors, including food, beverage, and pharmaceutical production. In the food industry, enzymes enhance flavor, texture, and nutritional value. In the beverage industry,enzymes assist in fermentation and clarification. In the pharmaceutical industry, enzymes contribute to thesynthesis of antibiotics, vaccines, and other life-saving medications.In conclusion, enzymes are indispensable molecules that perform a multitude of vital functions in all living organisms. Their catalytic prowess, substrate specificity, and remarkable selectivity make them the cornerstones of cellular processes. By understanding and harnessing the power of enzymes, we unlock a treasure trove ofpossibilities for improving human health and advancing scientific frontiers.中文回答:酶,生命的幕后英雄。
diet modification定义 -回复
diet modification定义-回复Diet modification –Understanding the Importance of Healthy EatingIntroductionDiet modification refers to making deliberate changes to one's eating habits to improve overall health and well-being. It involves adopting a balanced and nutritious diet while reducing the consumption of unhealthy foods and beverages. The goal of diet modification is to provide the body with essential nutrients, maintain a healthy weight, and reduce the risk of chronic diseases. In this article, we will discuss the importance of diet modification, the essential steps to implement it, and the long-term benefits it can bring.Why is Diet Modification Important?1. Nutritional BalanceA well-balanced diet provides the body with the necessary nutrients, including carbohydrates, proteins, fats, vitamins, and minerals, in appropriate proportions. These nutrients play vitalroles in maintaining bodily functions, supporting growth and development, and preventing nutritional deficiencies. Diet modification ensures that you consume a wide variety of foods from different food groups, promoting optimal health.2. Weight ManagementOne of the significant aspects of diet modification is weight management. Controlling portion sizes, choosing nutrient-dense foods, and limiting the intake of high-calorie foods helps in maintaining a healthy weight. Obesity and overweight are major risk factors for various chronic diseases, including heart disease, type 2 diabetes, and certain types of cancers. By managing weight through diet modification, individuals can significantly reduce the risk of such diseases.3. Chronic Disease PreventionA well-planned and healthy diet plays a crucial role in reducing the risk of chronic diseases. Consuming a diet rich in fruits, vegetables, whole grains, lean proteins, and healthy fats, while limiting processed foods, sugary drinks, and excessive salt intake, can help prevent conditions like obesity, hypertension, cardiovascular diseases, and certain cancers. Diet modification can also help inmanaging existing conditions like diabetes and hypertension by improving blood sugar control and reducing blood pressure levels.Steps to implement Diet Modification1. Evaluate Current Eating HabitsBegin by assessing your current eating patterns. This includes identifying the types of foods consumed, portion sizes, eating frequency, and any unhealthy habits such as excessive snacking or reliance on processed foods. This evaluation provides insights into potential areas for improvement and assists in setting realistic goals.2. Set Realistic GoalsBased on the evaluation, set specific, measurable, attainable, relevant, and time-bound (SMART) goals for yourself. For example, a goal could be to add one serving of vegetables to your lunch and dinner meals, reduce sugary beverage intake to once a week, or cook meals at home thrice a week. These objectives should be challenging yet achievable to sustain motivation and progress.3. Make Gradual ChangesInstead of completely overhauling your diet, approach modification gradually. Start by incorporating small changes to make them manageable and sustainable over the long term. For instance, try replacing sugary snacks with fresh fruits, opting for whole-grain products instead of refined grains, or switching from whole milk to low-fat alternatives. As you adapt to these changes, you can gradually implement further modifications.4. Seek Professional GuidanceIf you find it challenging to navigate through the vast amount of nutrition information available, consider consulting a registered dietitian or nutritionist. These professionals can provide personalized guidance, create customized meal plans, and help you address any specific dietary needs or concerns you may have.5. Focus on SustainabilityDiet modification is not a temporary fix but rather a sustainable lifestyle change. Emphasize adopting eating patterns that you can maintain in the long run. This involves cultivating healthy habits, such as meal prepping, mindful eating, and regular physical activity. Remember, consistency is key in achieving long-term success.The Long-Term Benefits of Diet ModificationBy implementing diet modification, individuals can experience numerous long-term benefits, including:1. Improved overall health and well-being2. Reduced risk of chronic diseases3. Enhanced weight management and body composition4. Increased energy levels and vitality5. Better mental and emotional health6. Strengthened immune system7. Increased lifespan and improved quality of lifeConclusionDiet modification is a crucial aspect of maintaining optimal health and preventing chronic diseases. By making gradual changes to your eating habits and focusing on a balanced and nutritious diet, you can lay the foundation for long-term well-being. Remember, healthy eating is a journey, and small steps towards improvement can have a significant impact on your overall health. So, embracediet modification, prioritize your well-being, and enjoy the benefits of a healthy lifestyle.。
卤酸脱卤酶样水解酶had的英文
卤酸脱卤酶样水解酶had的英文Haloacid Dehalogenase-like Hydrolytic Enzymes (HAD)。
Haloacid dehalogenase (HAD)-like hydrolytic enzymes are a large and diverse family of enzymes that catalyze the hydrolysis of a variety of substrates, including haloalkanoic acids, N-acylhomoserine lactones, and phosphonates. HAD enzymes are found in all three domains of life and play important roles in various metabolic pathways, including the degradation of xenobiotic compounds and the biosynthesis of secondary metabolites.HAD enzymes are typically composed of a single polypeptide chain that folds into a two-domain structure. The N-terminal domain is responsible for substrate binding, while the C-terminal domain contains the catalytic site.The catalytic site of HAD enzymes is characterized by the presence of a conserved triad of amino acids, consisting of a histidine, an aspartate, and a glutamate.The mechanism of action of HAD enzymes is well understood. The first step in the reaction is the binding of the substrate to the enzyme. The substrate is then positioned in the active site, where it is attacked by the catalytic triad. The histidine residue acts as a general base, abstracting a proton from the substrate. The aspartate residue then acts as a nucleophile, attacking the substrate carbonyl carbon. The glutamate residue stabilizes the transition state of the reaction.The hydrolysis of the substrate by HAD enzymes results in the formation of two products: a halide ion and an alcohol or carboxylic acid. The halide ion is released from the enzyme, while the alcohol or carboxylic acid remains bound to the enzyme. The alcohol or carboxylic acid is then released from the enzyme by a second hydrolysis reaction.HAD enzymes are important enzymes that play a variety of roles in metabolism. They are found in all three domains of life and are essential for the degradation of xenobiotic compounds and the biosynthesis of secondary metabolites.Applications of HAD Enzymes.HAD enzymes have a wide range of potential applications, including:The bioremediation of contaminated soils and groundwater.The production of biofuels and other renewable energy sources.The synthesis of pharmaceuticals and other fine chemicals.The development of new antibiotics and other antimicrobial agents.HAD Enzymes and Human Health.HAD enzymes are also important for human health. They play a role in the metabolism of drugs and other xenobiotics, and they are involved in the biosynthesis ofessential metabolites. Mutations in HAD genes have been linked to a number of human diseases, including cancer and neurodegenerative disorders.Conclusion.HAD enzymes are a large and diverse family of enzymes that play important roles in metabolism. They are found in all three domains of life and have a wide range of potential applications. HAD enzymes are also important for human health, and mutations in HAD genes have been linked to a number of human diseases.。
羧甲基纤维素的制备
羧甲基纤维素的制备英文回答:Carboxymethyl cellulose (CMC) is a cellulose derivative that is widely used in various industries due to its unique properties. It is prepared by the reaction of cellulose with sodium chloroacetate, which introduces carboxymethyl groups onto the cellulose backbone. The synthesis of CMC involves several steps.First, cellulose is typically derived from natural sources such as wood pulp or cotton fibers. It is important to note that cellulose is a linear polymer composed of glucose units linked by β-1,4-glycosidic bonds. The starting material, cellulose, needs to be in the form of a fine powder or fibers for the subsequent reactions.Next, the cellulose is treated with sodium hydroxide (NaOH) to activate the hydroxyl groups on the cellulose chain. This step is crucial as it increases the reactivityof cellulose towards the carboxymethylation reaction. The cellulose is then washed to remove any impurities and excess NaOH.After the activation step, the cellulose is reacted with sodium chloroacetate (NaClO2CH2COONa) in the presence of an alkaline catalyst such as sodium hydroxide. This reaction is carried out under controlled conditions of temperature and time. The carboxymethylation reaction involves the nucleophilic substitution of the hydroxyl groups on the cellulose chain by the carboxymethyl groups from sodium chloroacetate.Once the reaction is complete, the product is neutralized to remove any unreacted sodium hydroxide or other impurities. The neutralization can be achieved by adding an acid such as hydrochloric acid (HCl) or acetic acid (CH3COOH) to the reaction mixture. The resulting CMC is then filtered, washed, and dried to obtain the final product.中文回答:羧甲基纤维素(CMC)是一种纤维素衍生物,由于其独特的性质,在各个行业广泛应用。
海工英语词汇[最新]
海工英语词汇AA-60 fire door A-60级防火门a-hundred-year return period 百年一遇abrasive (喷砂用的)磨料abrasive paper 砂纸AC generator 交流发电机AC motor 交流电动机accelerated corrosion testing 加速腐蚀试验acceptance criteria 合格准则access hole (for welding) = cope hole (焊接)工艺孔accommodation and power platform (APP) 生活动力平台according to = in accordance with =in line with = as per = in the light of 按照acetylene gas 乙炔acid electrode 酸性焊条acid proof cement 防酸水泥additive 添加剂adhere to 遵守(动词)adherence to 遵守(名词)adjacent columns 相邻立柱adverse combination of loads 载荷的不利组合adverse condition 不利条件aeronautical radio system 航空无线电系统AFC (approved for construction) 建造批准aforementioned 上述aft winch 船艉绞车agitator 搅拌器air blower 鼓风机air compressor 空气压缩机air driven pump 气动泵air hoister 气动绞车air manifold 气包air tight test 气密性试验air-hose 风带aircraft obstruction beacon 航空障碍灯alarm buzzer 报警蜂鸣器alarm horn 报警喇叭aliphatic polyurethane 脂肪族聚氨脂漆allowable (working) stress 许用应力Aluminum alloy anode 铝合金阳极Aluminum ingot 铝锭aluminum paint 银粉漆aluminum sheet 铝皮ambient air temperature 环境气温ambient temperature 环境温度anchor block 地锚块anchor bolt 地脚螺栓anchor ground 锚地anchor pile 锚桩anchor profile/pattern 涂装表面的粗糙度anchor rack 锚架anchoring buoy 锚泊浮筒angle of incidence 入射角angle of reflection 折射角angle probe method 超声波斜射探伤angle steel 角钢angle valve 角阀anode core 阳极芯子anode end face geometry 阳极端面几何形状anode life 阳极寿命anode potential 阳极电位anode stand-off post 支架式阳极的立柱anode resistance 阳极电阻anti-electrostatic floor 防静电地板anticorrosive paint 防锈漆antipollution measures 防污染措施API specified requirements API规定的要求apparent batter 视斜度applicable API specification API适用规范approach-departure sector 直升飞机起落扇形区appurtenance = attachment= accessory= auxiliary 附件arc air gouging 碳弧气刨arc cutting 电弧切割arc strike 弧击argon arc welding 氩弧焊argon arc welding machine 氩弧焊机articulated tower mooring system 铰接塔系泊系统artificial island 人工岛as per 按照as-built 完工as-built dossier 完工资料as-built drawing 完工图as-rolled 轧制状态assemble 组装(动词)assembly 组装(动词)associated gas 伴生气at the option of A 按A的意愿atmospheric diving system= unpressurized diving system 常压潜水系统atmospheric vent 放空管attachment 附件auto fire alarm system 火灾自动报警系统automatic spraying system 自动喷淋系统B(to be) buttered (焊接的)长肉back chipping 清根back gouging 反面气刨backing weld 打底焊道backpressure regulator (self contained) 背压式调节阀(自力式)backwash return pump 反冲洗回流泵backwash surge tank 反冲洗罐Ball hardness 布氏硬度ball valve 球阀bar (截面较小的)圆钢方钢六角钢等bar chart 统计用的柱状图barge bumper 靠船件barge strength and stability 驳船强度及稳性barrel (管件的)加厚段base metal 母材、基本金属basic design 基本设计basic electrode = lime type covered electrode 碱性焊条battery room 蓄电池间blowout preventer system (BOP system) 防喷器系统beam depth 梁高beam path distance 超声波发射程距bearing strength of the ground 地基承载力bearing stress 支撑应力before welding is initiated 始焊前bending moment 弯矩bending stress 弯曲应力bevel 坡口bevel angle 坡口面角度beveling / chamfering 开坡口bid drawing 招标图纸bilge / fire pump 舱底污水-消防两用泵bilge alarm system 污水报警系统bid / tender for…投标blast cleaning 喷砂除锈blind flange 盲法兰blind spectacle flange 快速盲法兰blow count 打桩的锤击数blowdown vessel 排放罐blowhole; gas pore 气孔blowout preventer stack 防喷器组buoyant turret mooring 浮筒转塔式系泊boat landing 登船平台bolt, nut and washer 螺栓、螺母及垫片bolted connection 螺栓连接boom 吊机扒杆boom angle 吊机倾斜角bore hole 镗孔boss with plug 带有丝堵的管座bottom echo 底面回声bottom sitting drilling platform 坐底式钻井船boundary condition 边界条件bow 船艏box beam 箱形梁box type joint 箱形节点brace 拉筋bracelet anode 手镯式阳极branch member 支杆breathing apparatus , respirator 防毒面具bridge 栈桥bridge (overhead) crane 天车吊brittleness 脆性brush coating 用毛刷涂漆buckle fastener 绑带bulb angle 球扁钢build-up girder 组合梁bulk head 隔板、舱壁burn through 烧穿burner 气焊工burr and flashing 飞边毛刺business and management 经营管理butt-welded seam 对接焊缝butterfly valve 蝶阀Ccable layer 敷缆船cable rack / tray 电缆托架caisson 沉箱caisson washing pump 洗舱泵caisson-type platform 沉箱式平台can (管件的)段cantilever beam 悬臂梁cantilever jack-up rig 悬臂自升式钻井船cantilever well module 悬臂式井口模块cap beam 帽梁capped steel 半镇静钢capsizing lever 倾覆力臂capsizing moment = overturn moment 倾覆力矩carbon arc air gouging 碳弧气刨carbon dioxide cylinder 二氧化碳气瓶carbon equivalent 碳当量carbon-dioxide(CO2) arc welding 二氧化碳气体保护电弧焊cargo master system 货油舱总监控系统cargo tank cleaning machine 洗舱机carrier (搬运)小车carry out = perform = conduct = execute 进行、执行catenary anchor leg mooring (CALM) 悬链锚腿系泊cathodic protection 阴极保护cellar deck 底甲板cellar shutdown panel (CSDP) 井口系统关断盘cement grout 水泥浆cementing vessel 固井船center line 中心线center of buoyancy 浮心center of floatation 漂心center of gravity 重心center punch 冲子central control room (CCR) 中央控制室central processing platform 中心处理平台centrifugal pump 离心泵centrifuger 离心机ceramic fibres 防火陶棉ceramic tiles 瓷砖certifying authority 第三方检验机构chain block 倒链chair with arm-rest 带扶手的半软椅chamfering 开坡口channel steel 槽钢charpy-V notch energy 夏比V形缺口韧性值charpy-V notch impact test 夏比V形缺口冲击试验chart datum 海图基准面check randomly 抽检check valve 止回阀checkered plate 花纹板chemical injection package 化学注入撬chemical injection system 化学注入系统chemical seal 化学密封chipping hammer 敲渣锤chisel 风铲chord member 弦杆christmas tree 采油树circumference 周长circumferential weld 环焊缝classification survey 入级检验clean water injection pump 清水注入泵clean water surge tank 清水缓冲罐clearance 间隙client 业主、顾客close drain tank 闭式排放罐closed / open circuit 闭 / 开路closed drain pump 闭式排放泵clustered well heads 丛式井口CO2 cylinder unit CO2气瓶组CO2 extinguisher CO2灭火器coal tar epoxy paint 含焦油环氧涂料coaming 挡水扁铁coating 涂装coating interval 涂漆间隔coefficient 系数cold formed pipe 冷卷管cold shut 冷隔cold work 冷加工、冷作collapse ring 加强环板column 立柱combustible gas detector 可燃气体探测器come-along 手扳葫芦commercial blast cleaning 普通级喷砂除锈commissioning 调试compile and maintain a document 编制和保存文件complete penetration groove weld 全熔透坡口焊completion riser 完井立管complex joint 复杂节点compliance with 符合(名词)comply with 符合(动词)composite rockwool ceiling 复合岩棉天花板composite rockwool panel 复合岩棉板concentric reducer 同心大小头conceptual design 概念设计concrete platform 混凝土平台condensate drum 冷凝液罐condensing water 冷凝水conductor 隔水套管conductor guide 套管导向cone 大小口、锥体conform to = stick to = adhere to 符合、遵守connection 节点、连接consumption rate 阳极消耗率continuous beam 连续梁continuous double fillet weld 双面连续贴角焊continuous welding 连续焊contract 合同contraction stress 收缩应力contractor 承包商control valve (accessories handwheel) 带手轮的控制阀control valve assembly 控制阀总成coordinates 坐标系coordination 协调cope hole (焊接)工艺孔corrective and preventive action 纠正和预防措施correlation curve 相关曲线corrosion allowance 腐蚀余量corrosion coupon 腐蚀挂片corrosion protection 防腐corrugated plate interceptor 斜板分离器couplant UT用的耦合剂coupling 联轴器、管箍crack 裂纹crane barge, crane vessel 起重船crane support 吊机支撑crater 焊口crawler crane 履带吊车criteria (验收)标准cross angle 交叉角cross section 横截面crow bar 撬杠cruciform bollard 单十字带缆柱crude cooler 原油冷却器crude filter 原油过滤器crude heat exchanger 原油换热器crude loading /export pump 原油外输泵crude loading pump 原油装载泵crude oil drain tank 原油排放舱crude preheat exchanger 原油预热器crude suction strainer 原油吸入滤器crude transfer 原油输送泵cup support 垫墩curing time (油漆的)固化时间current capacity (阳极的)电流容量current density (阳极的)电流密度current direction 海流方向curvature radius 曲率半径customer 客户cut by torch 以火焰切割cut off 割除、割除物cutoff allowance 切割裕量cutting plan 排板图cutting sheet 单件图D(in) duplicate 一式两份(to) drive piles 打桩DAC curves 距离-振幅曲线daily diesel filter 日用柴油滤器damage-prone connection 易破坏的节点damper, dampener 减震器DC generator 直流发电机Decibel (dB) 分贝deck beam 甲板梁deck cover 甲板敷料deck crane 甲板吊车deck water seal unit 甲板水封装置deep freeze compressor 深冷压缩机deepwater jacket 深水导管架defect /default /disconnection 缺陷deflection 挠度dehydration degassing vessel 脱水除气器dehydration preheater 脱水预热器dehydration feed pump 电脱水供给泵delivery of materials 材料交货derrick barge 回转式起重船desander 除砂罐design basis 设计基准design parameters 设计参数detail 详图detail design 详细设计develop / establish a document 编制文件dew point 露点DFT (dry film thickness) 干膜厚度diagonal brace 斜拉筋dial gauge 百分表diaphragm 隔板diaphragm valve 隔膜阀die stamp 钢印diesel daily tank 柴油日用罐diesel filter coalescer 柴油过滤净化装置diesel generator set 柴油发电机组diesel oil daily tank with heater 柴油日用罐(带加热器)diesel pump 柴油泵diesel storage tank 柴油储罐diesel storage tank with heater 柴油储罐(带加热器)diesel tank 柴油罐diesel transfer pump 柴油输送泵differential pressure control valve 差压控制阀diffuser 布风气dihedral angle 二面角dimensional checks 尺寸检查direct / alternating current arc welding 直/交流电弧焊direct current emergency supp。
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Mathematische Annalen manuscript No.(will be inserted by the editor)Craig Huneke·Roger WiegandCorrection to“Tensor Products of Modules and the Rigidity of Tor”, Math.Annalen,299(1994),449–476.Received:July30,2006Abstract This note makes a correction to the paper“Tensor products of modules and the ridigity of Tor”,a correction which is needed due to an incorrect convention for the depth of the zero module.Mathematics Subject Classification(2000)13C12,13C14·13D02, 13H101CorrectionIn the paper[4],a convention for the depth of the zero module was made which is incorrect and affects several statements and proofs in the paper.The depth of the zero module was set to be−1,instead of the correct convention, which is∞.This has unexpected consequences in the statements of some of the results.Recall from[2]that afinitely generated module M over a Noetherian ring R is said to satisfy condition(S n)ifdepth(M P)≥min{n,dim(R P)}for every P∈Spec(R).Using the convention that the depth of the module0is−1implies that a module M can satisfy(S n)only if the support of M is all of Spec(R),which is not what is needed for various statements in the text.In particular,if R Craig HunekeDepartment of MathematicsUniversity of KansasLawrence,KS66045E-mail:huneke@Roger WiegandDepartment of MathematicsUniversity of NebraskaLincoln,NE68588E-mail:rwiegand@2Craig Huneke,Roger Wiegand is Gorenstein,then[2,3.6]gives that a module satisfies S2if and only if it is reflexive,and this characterization requires the convention that the depth of the zero module be∞.Another example is in application of the depth lemma.If0→M→N→K→0is a short exact sequence offinitely generated modules over a Noetherian local ring R,and depth(N)>depth(K),then depth(M)=depth(K)+1. This is clearly false under the convention that the depth of0be−1,e.g., if K=0.This necessitates additional arguments in some cases,e.g.,when localizing a short exact sequence at a prime not in the support of one of the modules.Changing the depth of the zero module to∞means that some statements need to be revised,and one proof in particular needs some more comment. The two results needing change are Corollary2.6and Theorem2.7.Corollary 2.6should be modified to the following statement:Corollary1Let R be a complete intersection,and let M and N be nonzero R-modules such that T or R i(M,N)=0for all i≥1.If M⊗R N is maximal Cohen-Macaulay,then so are M and N.The proof of this statement follows directly from Proposition2.5,as in the text.Proposition2.5states:Proposition1Let R be a complete intersection.Let M and N be non-zero finitely generated R-modules such that T or R i(M,N)=0for all i≥1.Thendepth(M)+depth(N)=dim(R)+depth(M⊗R N).One needs to note here that M⊗R N cannot be zero since both M and N are nonzero,and R is local.The only change in Corollary2.6is the deletion of the additional assertion that if M⊗R N satisfies Serre’s property(S n),then so do M and N.This is true if the support of both modules is entire spectrum,but could fail otherwise.Likewise,Theorem2.7should be contracted to the following statement:Theorem1(Second Rigidity Theorem)Let R=S/(f)be a hypersurface, and let M and N be non-zero R-modules,at least one of which has constant rank.If M⊗R N is reflexive,then T or R i(M,N)=0for all i≥1.The additional conclusion that both M and N are reflexive is never used in the text(except in low dimension,in which case the fact that they are maximal Cohen-Macaulay follows from(2.6)).The problem with this ad-ditional conclusion is that if N P=0,then the hypothesis is satisfied,but one cannot necessarily conclude anything about M P.One can only conclude results about both modules on the intersection of their supports.The proof of(2.7)must be modified in a few spots to take into account localization at a prime not in the support of one of the modules,but this isErratum3 easy to do.One main place this problem arises is in the argument that from the short exact sequence0→M⊗R N→N(n)→M1⊗R N→0one can conclude that the associated primes of M1⊗R N are all of height at most one.The proof given has a gap in case a prime P is not in the support of the tensor product M⊗R N.One must argue separately that if P is associated to M1⊗R N,and if(M⊗R N)P=0,then P is also associated to N,and hence has height zero as N is torsion-free.Corollary2.6and Theorem2.7are used in the paper[5]and similar modifications must be made,e.g.,in[5]pgs.169–172.Acknowledgements We thank Hailong Dao for alerting us to this error,finding places where it was a problem,and for also pointing out that there is not an established convention for what(S n)means for modules.We use the convention from[2]as stated above.In[3,Def.5.7.2]and in[1,Sec.2.1],a module is defined to satisfy(S n)ifdepth R(M P)≥min{n,dim(M P)}for every P∈Spec(R).PThis is weaker than the version we use.References1.Bruns,W.and Herzog,J.:Cohen-Macaulay Rings,Cambridge Studies in Ad-vanced Mathematics,39.Cambridge University Press,Cambridge,(1993).2.Evans,E.G.and Griffith,P.:Syzygies.Cambridge University Press,Cambridge.London Math.Soc.Lect.Notes Ser.106(1985)3.Grothendieck,A.and Dieudonn´e,J.:´El´e ments de G´e om´e trie Alg´e brique IV,Partie2.Publ.Math.I.H.E.S.24(1967).4.Huneke,C.and Wiegand,R.:Tensor products of modules and the rigidity ofTor.Math.Ann.299(1994),449–476.5.Huneke,C.and Wiegand,R.:Tensor products of modules,rigidity and localcohomology.Math.Scan.81(1997),161–183.。