菠萝pepc基因家族生物信息学分析
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摘 要：磷酸烯醇式丙酮酸羧化酶（phosphoenolpyruvate carboxylase, PEPC）在 C4 和景天酸代谢（CAM）光合途径吸 收 CO2 过程中发挥重要作用，并参与各种非光合作用过程，包括果实成熟、气孔开放、碳氮相互作用、种Fra Baidu bibliotek形成和萌 发以及调节植物对逆境胁迫的耐受性。菠萝为典型的景天酸代谢途径（CAM）植物，为了解磷酸烯醇式丙酮酸羧化酶 （PEPC）在菠萝景天酸代谢途径（CAM）中的作用，本研究鉴定到 3 个菠萝 PEPC 基因，按照基因描述命名为 AcPEPC1、AcPEPC3 和 AcPEPC4，进化树分析结果 AcPEPC1 和 AcPEPC3 为植物型 PEPC（PTPCs）蛋白，序列同 源性较高且基因结构、保守结构域及保守基序均一致。AcPEPC4 为细菌型 PEPC（BTPCs）蛋白，与 AcPEPC1/AcPEPC3 间的序列同源性低。组织表达分析表明 AcPEPC1 菠萝叶片表达量较高，而 AcPEPC3 和 AcPEPC4 在菠萝花和果实表 达量较高。 关键词：菠萝；磷酸烯醇式丙酮酸羧化酶（PEPC）；基因进化；基因表达谱 中图分类号：S668.3 文献标识码：A
热带作物学报 2020, 41(1): 097103 Chinese Journal of Tropical Crops
菠萝 PEPC 基因家族生物信息学分析
马海洋1,2，赵秋芳1,2,3*，陈 曙1,3，石伟琦1,2，冼皑敏1,2
1. 中国热带农业科学院南亚热带作物研究所，广东湛江 524091；2. 海南省热带作物营养重点实验室，广东湛江 524091； 3. 农业农村部热带果树生物学重点实验室，广东湛江 524091
Abstract: Phosphoenolpyruvate carboxylase (PEPC) plays an important role in assimilating atmospheric CO2 during C4 and Crassulacean acid metabolism (CAM) photosynthesis, and participates in various nonphotosynthetic processes, including fruit ripening, stomatal opening, supporting carbon-nitrogen interactions, seed formation and germination, and regulation of plant tolerance to stresses. Pineapple is a typical CAM plant. In order to understand the function of PEPC gene in pineapple CAM photosynthesis pathway, three PEPC genes were identified in pineapple and nominated as AcPEPC1/AcPEPC3/AcPEPC4 according to the gene description. Phylogenetic analysis showed that AcPEPC1 and AcPEPC3 protein belonged to the plant type PEPC (PTPCs) subfamily, which had high sequence homology. Gene structure, conserved domain and conserved motif of AcPEPC1 and AcPEPC3 had high similarity. AcPEPC4 belonged to the bacterial type of PEPC (BTPCs) subfamily which had low sequence homology with AcPEPC1/AcPEPC3. Different tissues expression assay showed that the expression of AcPEPC1 was high in leaves, and AcPEPC3 and AcPEPC4 was highly expressed in flowers and fruits. Keywords: pineapple (Ananas comosus (L.) Merr); phosphoenolpyruvate carboxylase (PEPC); gene evolution; gene expression profiling DOI: 10.3969/j.issn.1000-2561.2020.01.014
Bioinformatics Analysis of PEPC Gene Family in Pineapple
MA Haiyang1,2, ZHAO Qiufang1,2,3*, CHEN Shu1,3, SHI Weiqi1,2, XIAN Aimin1,2
1. South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China; 2. Hainan Key Laboratory of Tropical Crops Nutrition, Zhanjiang, Guangdong 524091, China; 3. Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs, Zhanjiang, Guangdong 524091, China
热带作物学报 2020, 41(1): 097103 Chinese Journal of Tropical Crops
菠萝 PEPC 基因家族生物信息学分析
马海洋1,2，赵秋芳1,2,3*，陈 曙1,3，石伟琦1,2，冼皑敏1,2
1. 中国热带农业科学院南亚热带作物研究所，广东湛江 524091；2. 海南省热带作物营养重点实验室，广东湛江 524091； 3. 农业农村部热带果树生物学重点实验室，广东湛江 524091
Abstract: Phosphoenolpyruvate carboxylase (PEPC) plays an important role in assimilating atmospheric CO2 during C4 and Crassulacean acid metabolism (CAM) photosynthesis, and participates in various nonphotosynthetic processes, including fruit ripening, stomatal opening, supporting carbon-nitrogen interactions, seed formation and germination, and regulation of plant tolerance to stresses. Pineapple is a typical CAM plant. In order to understand the function of PEPC gene in pineapple CAM photosynthesis pathway, three PEPC genes were identified in pineapple and nominated as AcPEPC1/AcPEPC3/AcPEPC4 according to the gene description. Phylogenetic analysis showed that AcPEPC1 and AcPEPC3 protein belonged to the plant type PEPC (PTPCs) subfamily, which had high sequence homology. Gene structure, conserved domain and conserved motif of AcPEPC1 and AcPEPC3 had high similarity. AcPEPC4 belonged to the bacterial type of PEPC (BTPCs) subfamily which had low sequence homology with AcPEPC1/AcPEPC3. Different tissues expression assay showed that the expression of AcPEPC1 was high in leaves, and AcPEPC3 and AcPEPC4 was highly expressed in flowers and fruits. Keywords: pineapple (Ananas comosus (L.) Merr); phosphoenolpyruvate carboxylase (PEPC); gene evolution; gene expression profiling DOI: 10.3969/j.issn.1000-2561.2020.01.014
Bioinformatics Analysis of PEPC Gene Family in Pineapple
MA Haiyang1,2, ZHAO Qiufang1,2,3*, CHEN Shu1,3, SHI Weiqi1,2, XIAN Aimin1,2
1. South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China; 2. Hainan Key Laboratory of Tropical Crops Nutrition, Zhanjiang, Guangdong 524091, China; 3. Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs, Zhanjiang, Guangdong 524091, China