柱式rna提取试剂盒操作流程

柱式rna提取试剂盒操作流程
1.从冰箱中取出所需的柱式RNA提取试剂盒，并等待试剂回温至室温。

Retrieve the required column RNA extraction kit from the refrigerator and wait for the reagents to warm up to room temperature.
2.将样本转移至离心管中，并加入相应的试剂混合均匀。

Transfer the sample to a centrifuge tube and add the corresponding reagents and mix well.
3.将混合样本转移至柱式RNA提取柱中。

Transfer the mixed sample to the column RNA extraction column.
4.将柱式RNA提取柱放置在2mL集尘管中。

Place the column RNA extraction column in a 2mL collection tube.
5.离心管放入高速离心机中离心并收集提取的RNA。

Centrifuge the centrifuge tube in a high-speed centrifuge and collect the extracted RNA.
6.在柱式RNA提取柱上加入DNAase，并进行离心去除DNA。

Add DNAase to the column RNA extraction column and centrifuge to remove DNA.
7.加入适量的乙醇，并离心洗涤提取的RNA。

Add an appropriate amount of ethanol and centrifuge to wash the extracted RNA.
8.将柱式RNA提取柱移至新的2mL集尘管中。

Move the column RNA extraction column to a new 2mL collection tube.
9.加入去离子水溶液，离心管中脱离RNA。

Add deionized water to the centrifuge tube to elute the RNA.
10.使用琼脂糖凝胶电泳或其他方法检测提取的RNA的质量和纯度。

Use agarose gel electrophoresis or other methods to
detect the quality and purity of the extracted RNA.
11.将提取的RNA存储在-80°C的冰箱中。

Store the extracted RNA in a -80°C refrigerator.。