反式白藜芦醇对Aβ25-35损伤大鼠海马旁回caspase-8、caspase-9的影响

反式白藜芦醇对Aβ25-35损伤大鼠海马旁回caspase-8、
caspase-9的影响
陈群;徐平;王顺旺;李彬;张丽;宋凯英
【期刊名称】《中国生化药物杂志》
【年(卷),期】2011(32)2
【摘要】目的观察反式白藜芦醇(TR)对β-淀粉样肤(Aβ25-35)损伤大鼠的海马旁
回的作用.方法大鼠随机分为假手术组、模型组、TR高、低剂量组、多奈哌齐对
照组在大鼠海马内注射Aβ25-35,后分别灌胃给相应药物10 d,采用TUNEL法检
测5组大鼠海马细胞及周围皮质凋亡情况;采用real time RT-PCR法检测5组大
鼠海马及皮质caspase-8、caspase-9 mRNA表达.结果大鼠海马及周边脑组织细胞凋亡情况,模型组与其他各组差异显著(P<0.01),说明造模成功.TR对caspase-8、caspase-9表达水平的下调非常显著(P < 0.01),低剂量组与高剂量组之间也有统计学差异(P<0.05).结论 TR对损伤的大鼠海马周围皮层神经元有保护作用,其机制可
能部分与下调caspase-8、caspase-9 mRNA表达有关.%Purpose To observe the effects of ingredient of polygonum cuspidatum trans-resveratrol (TR)
on injured rats parahippocampal gyrus. Methods Rats were randomly divided into 5 groups:the sham group, model group, TR high, low dose group and Aricept ( Donepezil Hydrochloride Tablets) control group. In the rat hippocampus after injection of Aβ25-35 ,the drugs was administrated
10 days,5 groups assay and the surrounding cortex hippocampal cell apoptosis by TUNEL, using real time RT-PCR detected 5 rat hippocampus and cortex expression of the caspase-8, caspase-9 mRNA. Results The rat
hippocampus and surrounding brain tissue apoptosis of model group was different to other groups (P ＜0.01 ). The model was developed. The expression level of TR on caspases-8 ,caspase-9 were different to other groups ( P ＜ 0.01 ). There was also different between TR high and low dose group( P ＜ 0.05 ). Conclusion hippocampus has a protective effect on injured rats parahippocampal gyrus, which may be partly reduced caspase-8,caspase-9 mRNA expression.
【总页数】4页(P115-118)
【作者】陈群;徐平;王顺旺;李彬;张丽;宋凯英
【作者单位】遵义医学院附属医院神经内科,贵州,遵义,563003;遵义医学院附属医院神经内科,贵州,遵义,563003;厦门市第三医院神经内科,福建,厦门,361100;遵义医学院附属医院神经内科,贵州,遵义,563003;遵义医学院附属医院神经内科,贵州,遵义,563003;遵义医学院附属医院神经内科,贵州,遵义,563003
【正文语种】中文
【中图分类】R966
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