Expression of heterologous multi-domain proteins i
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专利名称：Expression of heterologous multi-domain proteins in yeast
发明人：Nalini Motwani,Robert Blackburn
申请号：US09574492
申请日：20000519
公开号：US06358733B1
公开日：
20020319
专利内容由知识产权出版社提供
摘要：This invention demonstrates the utility of a yeast expression system for the expression of functional heterologous multi-domain proteins in yeast. The yeast expression system allows for the inclusion of a plurality of (up to three) modular expression cassettes which may encode multiple polypeptide chains of a heterologous multi-domain protein on a single plasmid (Twin Cassette). Because multiple polypeptide chains may be encoded for by the expression cassettes of the present invention in a single vector, the system can produce equivalent amounts of the multiple polypeptide chains, thereby enhancing the yield of a functional heterologous multi-domain protein. For example, functional monoclonal antibodies (MAbs) comprising a heavy chain and a light chain of an immunoglobulin (IgG), and functional immunotoxins comprising an antibody domain and an oxidase toxin may be produced using the Yeast expression system of the present invention. In addition, functional single chain antibodies, antibody fragments and chimeric antibodies may also be produced.
申请人：APOLIFE, INC.
代理机构：Baker Botts, LLP
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