载体元件指南

载体元件指南
哺乳动物⼴泛
性启动⼦
名称描述应⽤注释
CMV Human cytomegalovirus immediate early
enhancer/promoter
Strong promoter; may have variable strength in some cell types.
EF1A Human eukaryotic translation elongation
factor 1 α1 promoter
Strong promoter.
EFS Human eukaryotic translation elongation
factor 1 α1 short form
Strong promoter.
CAG CMV early enhancer fused to modified chicken β-actin promoter
Strong promoter.
CBh CMV early enhancer fused to modified chicken β-actin promoter
Strong promoter.
SV40Simian virus 40
enhancer/early promoter Medium-strength promoter. mPGK Mouse phosphoglycerate kinase 1 promoter
Medium-strength promoter. hPGK Human phosphoglycerate
UBC Human ubiquitin C promoter Weak promoter.
注意 :
有些元件会有不同
的序列版本，但功
能是⼀致的。

VectorBuilder根
据不同的⾻架需
要，使⽤不同的序
列版本。

CAG和CBh含有不同
序列版本的
chicken β-actin
启动⼦。

哺乳动物组织
特异性启动⼦
名称描述组织特异性Nanog Mouse nanog promoter Embryonic inner cell mass
Nes Rat nestin intron 2
enhancer fused to mouse
Hsp68 minimal promoter
Nervous system at embryonic
stage
Tuba1a Rat α1A tubulin promoter Nervous systems at embryonic and early postnatal stages Camk2a_long Mouse α-calcium-calmodulin dependent kinase II
promoter (8 kb)
Forebrain
Camk2a_short Mouse α-calcium-calmodulin dependent kinase II
promoter (1.3 kb)
Brain
SYN1Human synapsin I promoter Brain
Hb9Mouse Hb9 enhancer fused to
mouse Hsp68 minimal
promoter
Spinal cord
NSE Rat neuron-specific enolase
promoter
Nervous system
GFAP_long Human glial fibrillary
acidic protein promoter
(2.1 kb)
Brain
GFAP_short Human glial fibrillary
acidic protein promoter
(0.68 kb)
Brain
Prnp Mouse prion protein
promoter
Brain, hippocampus, cerebellum,
retina
Cnp Mouse 2′,3′-cyclic
nucleotide 3′-
phosphodiesterase promoter
Nervous systems, testis and
thymus
K14Human keratin 14 promoter Epidermis BK5Bovine keratin 5 promoter Epidermis cTnT Chicken cardiac troponin T promoter
Heart
alphaMHC_long Mouse α-cardiac myosin
heavy chain promoter (5.4
kb)
Heart
alphaMHC_short Mouse α-cardiac myosin
heavy chain promoter (2.8
kb)
Heart
Myog Mouse myogenin promoter Muscle
ACTA1Human skeletal muscle actin
SM22a Mouse transgelin (a.k.a.
Sm22a) promoter
Adult smooth muscle
Runx2Mouse runt related
transcription factor 2
promoter
Bone
OC Human osteocalcin promoter Bone and cartilage
Col1a1Rat collagen type I α1
promoter
Bone, tooth and tendon
Col2a1Mouse collagen type II α1
promoter
Cartilage
aP2Rat adipocyte P2 promoter Fat Adipoq Mouse adiponectin promoter Fat Tie1Mouse tyrosine kinase with immunoglobulin-like and
EGF-like domains 1 promoter
Blood vessel
Cd144Mouse cadherin 5 (a.k.a.
VE-Cadherin) promoter
Blood vessels
CD68_short Human CD68 promoter, short, recommended for in vitro
use
Blood
CD68_long Human CD68 promoter, long, recommended for in vivo use Blood
Afp Mouse α-fetoprotein
enhancer II fused with
human beta globin promoter
Liver
Alb Mouse albumin promoter Liver
TBG Human thyroxine-binding
globulin promoter
long terminal repeat (LTR)
Mammary gland
Wap Mouse whey acidic protein promoter
Mammary gland
Pdx1Mouse pancreatic and
duodenal homeobox 1
promoter
Embryonic developing pancreas
and adult pancreatic islets
{Ins2 promoter}Pancreas
Hcn4Mouse hyperpolarization-activated cyclic
nucleotide-gated K+ 4
Embryonic heart
NPHS2Human podocin promoter Kidney CD144Human cadherin 5 (a.k.a.
VE-Cadherin) promoter
Lung, heart, ovary, spleen and
kidney glomeruli
诱导型启动⼦
名称描述应⽤注释
TRE Tetracycline-responsive
element promoter (2nd
generation)
Regulated by a class of
transcription factors (e.g.
tTA, rtTA and tTS) whose
activities are dependent on tetracycline or its analogs
TRE3G Tetracycline-responsive element promoter (3rd
generation)
or its analogs (e.g. doxycycline); low background
GAL1Saccharomyces cerevisiae galactokinase promoter Repressed in the presence of glucose; induced in the
presence of galactose.
MET17Saccharomyces cerevisiae O-acetyl homoserine-O-acetyl
serine sulfhydrylase
promoter; aka MET25 and
MET15
Repressed in the presence of methionine.
CUP1Saccharomyces cerevisiae metallothionein promoter
Induced in the presence of copper.
AOX1Methanol-induced alcohol oxidase 1 promoter from
Pichia pastoris
Maximally induced during growth
on methanol and fully repressed during growth on glucose or glycerol.
斑马鱼⼴泛性
表达启动⼦
名称描述应⽤注释
sCMV Simian cytomegalovirus immediate early
enhancer/promoter
Strong promoter.
bactin2Zebrafish cytoskeletal
Ubi Zebrafish ubiquitin (ubi)
promoter
Ubiquitous and constitutive promoter.
斑马鱼组织特
异性启动⼦
名称描述组织特异性
cmlc2Cardiac myosin light chain
2 promoter
Heart
zK5Zebrafish Keratin 5
promoter
Tongue and fin
503unc 503-bp promoter of
zebrafish unc-45b
Muscle
斑马鱼诱导型
启动⼦
名称描述应⽤注释
hsp70Zebrafish heat-shock
protein 70 promoter
Strong and heat-shock inducible promoter.
5×UAS Five tandem upstream activation sequence (UAS)
followed by E1B minimal
promoter
Activates gene transcription
upon binding with GAL4 protein.
植物组成型启
动⼦
名称描述应⽤注释
CaMV 35S_short Cauliflower mosaic virus
drives high-level gene
expression in dicot plants but
is less effective in monocots.
CaMV 35S_long Cauliflower mosaic virus
35S promoter (long)
Strong promoter.
CaMV 35S_enhanced A chimeric promoter with
the doubled CaMV 35S
enhancer element
Strong promoter.
Nos Agrobacterium nopaline
synthase promoter
Weaker promoter compared to
CaMV 35S promoter.
ZmUbi Maize ubiquitin promoter Strong constitutive promoter, drives high-level gene expression in monocots.酵母组成型启
动⼦
名称描述应⽤注释
TEF1Saccharomyces cerevisiae
translation elongation
factor EF-1 alpha promoter
Strong promoter.
GPD Saccharomyces cerevisiae
glyceraldehyde-3-phosphate
dehydrogenase promoter
Strong promoter.
ADH1_short Saccharomyces cerevisiae
alcohol dehydrogenase
promoter
Weak promoter (stronger than
ADH1_medium and ADH1_long).
ADH1_medium Saccharomyces cerevisiae
alcohol dehydrogenase
ADH1_long).
ADH1_long Saccharomyces cerevisiae alcohol dehydrogenase
promoter
Weak promoter.
GAP Glyceraldehydes-3-phosphate dehydrogenase promoter from
Pichia pastoris
Strong constitutive promoter.
果蝇⼴泛性表
达启动⼦
名称描述应⽤注释
Actin 5C The distal promoter of cytoskeletal actin 5C gene
of Drosophila melanogaster, controling synthesis of
actin in a tissue- and developmental stage-
specific manner
Strong promoter
Polyubiquitin Promoter of polyubiqutin gene of Drosophila
melanogaster
Strong promoter
alpha 1-tubulin Promoter of α1-tubulin gene of Drosophila
melanogaster
Medium-strength promoter
果蝇组织特异
性启动⼦
名称描述组织特异性
Rh2Promoter of Rh2 gene of Drosophila melanogaster
动⼦
名称描述应⽤注释
Mtn Promoter of the metallothionein gene of Drosophila melanogaster Strong promoter; tightly indubible with the presence of Gu+.
参考⽂献序列
Gene Expr Patterns. 9:54
View (2009); Biotechnol Lett.
33:1319 (2011)
Dev Biol. 192:289 (1997)View Development. 138:169 (2011)View
参考⽂献序列Development. 127:1953 (2000); View Nat Methods. 7:219 (2010) Cell. 52:169 (1988); Nat
View Methods. 7:219 (2010)
参考⽂献序列
EMBO J. 3:3311 (1984); Yeast. View 27:955 (2010)
参考⽂献序列
Mol Cell Biol. 10:6172 (1990); Nucleic Acids Res. 19:5037 (1991)
View
Mol Cell Biol. 8:4727 (1988);
Methods Mol Biol. 62:385
(1997)
View
Nucleic Acids Res. 19:5037
(1991)
View
细胞类型特异性参考⽂献序列
Ocellar photoreceptor cells Genetics. 120:173 (1988)
View
参考⽂献序列Nucleic Acids Res. 19:5037 (1991); Genetics. 112:493
(1986)
View。