蓝色实验室试管实验ppt模板免费下载
试管婴儿胚胎实验室操作流程PPT
Day 0:取卵当天 取卵:
在这个过程中所用的冲洗液为预热的G-MOPS 培养液(使用前确保温度在37℃) • 此时卵泡液有的是蛋白,所以用的是不加蛋白的G-MOPS • 卵泡抽取物应该收集到无菌无毒性的一次性组织培养用试管中,并立即由实
B.4孔或者5孔板
a)HYSAETM溶液
b-f)G-MopsTMPlus
3.使用大口径的吸管,将3~5枚卵母细胞放进稀释的HYASE中。轻轻吸打,不要超过30s 4.将部分剥离的卵母细胞转移至第一冲洗液中,轻轻吹打每一个卵母细胞以除去放射冠,其 余液滴中重复此项操作 5.观察卵母细胞中是否有极体(有极体-M2;无极体-M1) • 成熟的M2,如果准备立即注射,置于ICSI液中 • 如果还需要孵育一段时间的,可以将卵母细胞置于已添加蛋白的G-1中直到开始注射
IVM
内容
未成熟卵母细胞体外成熟(IVM)
定义:“获取窦卵泡中的未成熟的颗粒细胞-卵母细胞复合体(COC),进行体外成熟”
(左)控制性超促排卵后PCOM/PCOS患者卵巢; (右)未行促排卵治疗的PCOM/PCOS患者
IVM技术主要针对卵子成 熟障碍,即所有卵子均不 能成熟,或者卵子成熟率 低(<20%)的患者,如 多囊卵巢综合征的患者、 促性腺激素刺激高反应患 者、卵巢低反应患者、需 要生育力保存的女性肿瘤 患者
第三天: 评估胚胎卵裂
移植或冷冻保存。
继续培养
第四天: 转移胚胎至新鲜的G-2TMPLUS液滴中 37℃,6% CO2过夜培养。
第五天早晨: 评估胚胎卵裂以确定移植或冷 冻保存
ICSI
医疗试管PPT模板
effects
• Phase IV:
– FDA mandated post-market safety trials7 4) McGrath, supra note 1, at 609. – Post-market treatment as 5) Karen E. Woodin, & John C. Schneider, TehexCpRAe'srGim uideetonMtoanittoiroingnClinical Research 37-38 (CenterWatch 2003).
Historical Context
• Regulation of drugs vs. regulation of clinical research
• Government regulation prompted by tragedies1
• Regulation of food and drugs:
Current Regulatory Framework
• Kefauver-Harris Amendments:
– Requires IND application4 – Requires minimal amount of safety5
• Three phases of clinical trials:6
Historical Context
• Regulation of clinical research:
– Nazi experiments Nuremberg Code – Other subject abuses:
试管 ppt模板
Science Fair ProjectType your project title hereYour nameYour teacher’s nameYour schoolStatement of the ProblemType your question here.(This is the question that your experiment answers.)Project OverviewType a brief overview or summary ofyour project here.ResearchSummarize your research here in three to five bullet points:•1st bullet point•2nd bullet point•3rd bullet point•4th bullet point•5th bullet pointVariables▪Controlled variables: These are the things that are kept the same throughout your experiments.▪Independent variable: The one variable that you purposely change and test.▪Dependent variable: The measure of change observed because of the independent variable. It is important todecide how you are going to measure the change.HypothesisBased on the research you have done, you will be writing an answer or solution –your best educated guess –to your question. Make sure you write down your hypothesis before you begin your experiment.Materials▪Type a detailed list of the items you needed to complete your experiments.▪Be specific about the amounts used.Procedure▪List all of the steps used in completing your experiment.▪Remember to number your steps.▪Add photos of your experiments.Data/Observations▪It is easier to understand the data if it is put into a table or graph.Create a graph in Microsoft Excel and import it here.▪Make sure all data is clearly labeled.ConclusionType a brief summary here of what you discovered based on the results of your experiments. You need to indicate whether or not the data supports the hypothesis and explain why or why not.Works CitedBe sure to include print and electronic sources and put them in alphabetical order.。
蓝色风格化学实验总结汇报PPT模板
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蓝色卡通化学实验课PPT课件模板
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蓝色医药实验PPT模板
Data Analysis
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