FPH2_HNMR_25029_MedChemExpress
HMN-214_SDS_MedChemExpress
Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :HMN-214Catalog No. :HY-12045CAS No. :173529-46-91.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureGHS Classification in accordance with 29 CFR 1910 (OSHA HCS)Acute toxicity, Oral (Category 4), H302Acute aquatic toxicity (Category 1), H400Chronic aquatic toxicity (Category 1), H4102.2 GHS Label elements, including precautionary statementsPictogramSignal word WarningHazard statement(s)H302 Harmful if swallowed.H410 Very toxic to aquatic life with long lasting effects.Precautionary statement(s)P264 Wash skin thoroughly after handling.P270 Do not eat, drink or smoke when using this product.P273 Avoid release to the environment.P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.P330 Rinse mouth.P391 Collect spillage.P501 Dispose of contents/ container to an approved waste disposal plant.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:IVX⁻214; HMN214; HMN 214; IVX 214; IVX⁻214; IVX214Formula:C22H20N2O5SMolecular Weight:424.47CAS No. :173529-46-94. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance White to off-white (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGUN number: 3077Class: 9Packing group: IIIEMS-No: F-A, S-FProper shipping name: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S.Marine pollutant: Marine pollutantIATAUN number: 3077Class: 9Packing group: IIIProper shipping name: Environmentally hazardous substance, solid, n.o.s.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
PCR抑制剂和增强剂
• PCR反应：扩增Bacteroides distasonis基因组 • 消除腐植酸抑制作用的最优BSA浓度是200-400ng/μl，最
优gp32浓度是100-150ng/μl • 因此，所有已知的抑制剂在检测时，都同时做了不含BSA
和gp32的检测和含有400ng/μl BSA或150ng/μl gp32的检测。
• 非离子去污剂，例如Tween®-20, NP-40和Triton® X-100等，可以 抵抗痕量的强离子去污剂的抑制作用，比如0.01%的SDS。
第十页，编辑于星期三：五点 五十二分。
3.用于优化引物和模板的结合
• 铵离子的添加，可以减少引物和模板的错配，提高反应的特 异性，可以让PCR对反应条件的要求更低，所以很多PCR试 剂都包含了10-20mM的硫酸铵。
• 1.用于扩增高GC含量或形成复杂二级结构的模板（共溶剂） • 2.用于保护DNA聚合酶的活性和稳定性
• 3.用于优化引物和模板的结合
第七页，编辑于星期三：五点 五十二分。
1.用于扩增高GC含量或形成复杂二级结构的模板 的增强剂
• 甜菜碱（betaine），二甲基亚砜（DMSO）和甲酰胺 （formamide），甘油，适合于扩增高GC含量和形成复杂二 级结构的模板。
自然黑发
离根部 11cm内
100%
离根部 11cm外
部分成功
离根部 16cm外
几乎不成功
白头发
离根部 31cm外
可成功
染成深咖啡 色的黑头发
完全失败
染成深咖啡 色的白头发
可成功扩增
第六页，编辑于星期三：五点 五十二分。
PCR增强剂
• PCR增强剂可以增加所需PCR产物的产量或减少非特异性产物。
Gelucire-14-44-SDS-MedChemExpress
Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:Nov.-23-2018Print Date:Nov.-23-20181. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :Gelucire 14/44Catalog No. :HY-Y1892CAS No. :121548-04-71.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:NoneFormula:N/AMolecular Weight:N/ACAS No. :121548-04-74. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Pure form-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance White to off-white (Oil)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2018 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
植物查尔酮合酶(CHS)酶联免疫分析使用说明书
植物查尔酮合酶(CHS) 酶联免疫分析使用说明书使用目的：本试剂盒用于测定植物组织，细胞及其它相关样本中查尔酮合酶(CHS) 活性。
实验原理本试剂盒应用双抗体夹心法测定标本中植物查尔酮合酶(CHS) 水平。
用纯化的植物查尔酮合酶(CHS) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入植物查尔酮合酶(CHS) ，再与HRP 标记的查尔酮合酶(CHS) 抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB 显色。
TMB 在HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的植物查尔酮合酶(CHS) 呈正相关。
用酶标仪在450nm 波长下测定吸光度（OD 值），通过标准曲线计算样品中植物查尔酮合酶(CHS) 活性浓度。
标本要求1．标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。
若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融2．不能检测含NaN3 的样品，因NaN3 抑制辣根过氧化物酶的（HRP）活性。
操作步骤1. 标准品的稀释：本试剂盒提供原倍标准品一支，用户可按照下列图表在小试管中进行稀释。
80 U/L5 号标准品150μl 的原倍标准品加入150μl 标准品稀释液40 U/L4 号标准品150μl 的5 号标准品加入150μl 标准品稀释液20 U/L3 号标准品150μl 的4 号标准品加入150μl 标准品稀释液10 U/L2 号标准品150μl 的3 号标准品加入150μl 标准品稀释液5 U/L1 号标准品150μl 的2 号标准品加入150μl 标准品稀释液2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、标准孔、待测样品孔。
在酶标包被板上标准品准确加样50μl，待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品最终稀释度为5 倍）。
加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。
n-乙基-2-异丙基-5-甲基-环己烷甲酰胺分子式_概述说明
n-乙基-2-异丙基-5-甲基-环己烷甲酰胺分子式概述说明1. 引言1.1 概述在有机化学中，n-乙基-2-异丙基-5-甲基-环己烷甲酰胺分子式（C9H19CONHCH(CH3)R）是一种重要的化合物。
它具有广泛应用于医药、农药和染料等领域的特性。
本文将对该化合物的结构、合成方法、物理化学性质以及安全性进行详细探讨。
1.2 文章结构本文共分为五个部分进行论述。
引言部分概述了文章的目的和主要内容，第二部分将解释n-乙基-2-异丙基-5-甲基-环己烷甲酰胺的化学名称和分子式，并介绍其结构特点和形式简化，还探讨了它在不同领域中的用途和应用。
第三部分将阐述常用的合成方法，并概述其工艺流程和关键步骤，同时提供改进方向和发展趋势。
第四部分将详细说明该化合物的理化性质与测定方法，并对其对环境和人体的影响进行安全性评价与探讨，最后给出安全使用与处理建议。
第五部分将对全文进行总结，并展望n-乙基-2-异丙基-5-甲基-环己烷甲酰胺研究的未来发展方向。
1.3 目的本文旨在全面了解和介绍n-乙基-2-异丙基-5-甲基-环己烷甲酰胺分子式，包括其化学名称、结构特点、合成方法和工艺流程，以及物理化学性质和安全性评价等方面的内容。
通过全面分析该化合物，期望能够为相关领域的研究提供参考和指导，同时引发对其未来研究的探讨和展望。
2. n-乙基-2-异丙基-5-甲基-环己烷甲酰胺分子式的解释：2.1 化学名称和分子式：n-乙基-2-异丙基-5-甲基-环己烷甲酰胺，其化学名称中的"n-"表示它是一种正构化合物，"乙基"表示分子中有一个两碳的侧链，"异丙基"表示分子中有一个三碳的侧链，而"s-"表示该侧链连接在主链上较低号位。
分子式为C12H23NO。
2.2 结构特点和形式简化：n-乙基-2-异丙基-5-甲基-环己烷甲酰胺是一种脂肪胺类化合物，它由一个环状的六元碳骨架（环己烷）和两个侧链（乙基和异丙基），以及一个与其中一个侧链相连的甲酰胺官能团组成。
检测氢离子的荧光探针：含稠合外环的硼-二吡咯亚甲基染料的合成、光谱和电化学性质研究
无
机
化
学
学
报
Ｖｏ ．７ ＮＯ６ １２ ． ｌ ７７— １ ４ １ １８
Ｃ Ｎ ＥＪ ＨＩ ＥＳ ＯＵＲＮ ＮＯＲ ＡＬ ＯＦ Ｉ ＧＡＮＩ Ｃ ＣＨＥＭＩ Ｔ Ｙ Ｓ Ｒ
检 测氢 离子 的荧 光探 针 ： 稠合外 环 学性 质 研 究 光
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Ｋｅ ｏ ｄ ： ｏｏ —ｉｙｒ ｔｅ ｅ（ Ｄ ） ｙ ｓ ｆｓ ｄｅ ｏ ｙ ｌ ｎ ｓ Ｈ ｒｃ ｇ ｉｏ ； ｌｃ ｏ ａ ｓ ｒ ｆ ｏｅｃ ｎ ｓｎ ｏ ｙｗ ｒ ｓ Ｂ ｒｎｄｐ ｒ ｍｅ ｎ Ｂ Ｐ ｄ ｅ； ｕ ｅ ｘ ｃ ｃ ｃｒ ｇ ； ｅ ｏ ｎｔ ｎ ｅｅ ｔ ｎｔ ｎ ｆ ， ｕ ｒｓｅ ｔ ｅ ｓｒ ｏ ｈ ｉ ｉ ｉ ｒ ｒ ｅ ｌ
氢离子滴加到 Ｎ， 二甲基苯胺取代的硼一 二吡咯亚 甲基 染料（） Ｃ 。Ｎ Ｈ ０ １ ，／） １的 Ｈ Ｃ — （： ｖｖ￣ １
在 可 见光 激发 下 ． 以作 为 酸 性 ｐ 范 围 内检 测 氢离 子 的荧 光 探 针 。 可 Ｈ
关 键 词 ： 二 吡咯 亚 甲基 染 料 ； 合外 环 ；氢 离 子识 别 ；电子 转 移 ； 光 探 针 硼一 稠 荧
Ａ ｓ ａ ｔ ｈｅ ｅ ｘｃｃｉ ｉｇｆｓｄ ｂ ｒｎｄｐｒ ｍｅ ｅ ｅ （Ｄ ） ｄ ｅ ｉ ， ｄｍｅ ｙａ ｉｎ，Ｐ ｂ ｔ ｃ：Ｔ ｒｅ ｎ ｗ ｅｏｙ ｌ ｒ — ｅ ｏｏ —ｉｙｒ ｔ ｎ Ｂ Ｐ ｙ ｓ ｗｔ ＮＮ－ｉ ｔ ｌｎｌ ｅ 。 ｒ ｃ ｎ ｕ ｏ ｈ ｈ ｈ ｉ
辅酶 II NADP(H)含量检测试剂盒(MTT 显色法)说明书__ 微量法UPLC-MS-4371
辅酶II NADP(H)含量检测试剂盒（MTT 显色法）说明书货号：UPLC-MS-4371规格：100T/48S产品内容：使用前请认真核对试剂体积与瓶内体积是否一致，有疑问请及时联系工作人员。
微量法试剂名称规格保存条件酸性提取液液体25mL×1瓶2-8℃保存碱性提取液液体25mL×1瓶2-8℃保存试剂一液体10mL×1瓶2-8℃保存试剂二粉剂×1支-20℃保存试剂三液体8mL×1瓶2-8℃保存试剂四A 粉剂×1支-20℃保存试剂四B 液体5mL×1瓶2-8℃保存试剂五液体30mL×1瓶2-8℃保存试剂六液体50mL×1瓶2-8℃保存NADP 标准品粉剂×1支-20℃保存NADPH 标准品粉剂×1支-20℃保存溶液的配制：1、试剂二：临用前加入4mL 蒸馏水充分混匀，溶解后4℃保存2-8周。
2、试剂四：临用前将试剂四A溶解到试剂四B 中，溶解后加入5mL 蒸馏水，分装保存，避免反复冻融，-20℃保存4周。
3、NADP 标准品：临用前加入1.27mL 蒸馏水，即5µmol/mL ，-20℃可以保存2周。
4、NADPH 标准品：临用前加入1.2mL 蒸馏水，即5µmol/mL ，-20℃可以保存2周。
产品说明：辅酶ⅡNADP(H)广泛存在于动物、植物、微生物和培养细胞中，NADP +和NADPH 含量测定可以计算NADP （NADPH +NADP +)含量和NADPH/NADP+比值，其变化与磷酸戊糖途径和生物合成以及抗氧化反应密切相关。
NADPH/NADP +比值不仅是细胞氧化还原态的主要标志之一，而且在PPP 途径、生物合成和抗氧化代谢中具有重要调控作用。
分别用酸性和碱性提取液提取样品中NADP +和NADPH 。
NADPH 通过PMS 的递氢作用，使氧化型噻唑蓝（MTT ）还原为甲瓒，570nm 下检测吸光值，从而测定NADPH 含量。
质量标准
复合磷酸酯酶拼音名：Fuhe Linsuanzhimei英文名：Phosphoesterase Complex书页号：D9-210 标准编号：WS-10001-(HD-0858)-2002本品系自大麦芽中提取的具有磷酸酯酶活性的物质。
按干燥品计算，每1mg含磷酸酯酶的效价不得少于3200单位。
【性状】本品为浅褐黄色粉末，微臭，味微酸。
【鉴别】取本品0.5g，置研钵中，加三羟甲基氨基甲烷缓冲液(pH7.0)(取三羟甲基氨基甲烷3.0g，加水400ml溶解，用0.1mol/L盐酸溶液调节pH值至7.0，用水稀释至500ml)10ml，充分研磨，匀浆液以7000转/分的转速离心10分钟，取上清液用定性滤纸过滤，取滤液作为供试品溶液：取供试品溶液1ml，加核糖核酸溶液(取核糖核酸2g，加水80ml，摇匀，用氢氧化钠溶液调节pH值至7.0～7.2使溶解，加水稀释至100ml，现配现用或－20℃冷冻保存)1ml与0.01mol/L氯化锌溶液0.5ml，混匀，置65.0℃±0.5℃水浴保温15分钟；以上述三羟甲基氨基甲烷缓冲液1ml代替供试品溶液同法操作作为对照。
保温结束后，各加0.005ml溴乙锭溶液(取溴乙锭1g，加水100ml，置磁力搅拌器搅拌数小时使其完全溶解，避光保存)，摇匀，于暗处在紫外灯(波长300nm)下观察，对照应显明亮的橙色荧光，供试品则不显荧光。
【检查】干燥失重取本品，在105℃干燥4小时，减失重量不得过8.0％(中国药典2000年版二部附录ⅧL)。
【效价测定】供试品溶液的制备取本品250mg，精密称定，置100ml量瓶中，加水溶解并稀释至刻度，摇匀，呈浑浊的溶液。
测定法取试管2支，分别精密加入核糖核酸溶液[取核糖核酸(含量80％以上)1.0g，加水30ml，摇匀，用氢氧化钠试液调节pH值至7.0～7.2，再加水稀释至50ml]3ml、三羟甲基氨基甲烷缓冲液[取三羟甲基氨基甲烷1.2g，加水50ml溶解后，用0.1mol/L的盐酸溶液(约85ml)调节pH值至7.0～7.2，加水稀释至200ml] 1.5ml、0.01mol/L的氯化锌溶液0.5ml，在70℃水浴中保温5分钟，分别精密加入供试品溶液1ml，摇匀，置70℃水浴中准确反应30分钟，再精密加入高氯酸钼酸铵试液(取钼酸铵0.25g，加70％～72％高氯酸3.5ml及水适量使溶解，用水稀释至100ml)5ml，摇匀，立即置冰浴中冷却15分钟，滤过，取续滤液2ml置100ml量瓶中，加水稀释至刻度，照分光光度法(中国药典2000年版二部附录ⅣA)，在260nm的波长处以水为参比测定吸收度A，以水1ml代替供试品溶液，同法操作，测定吸收度A。
hmda 分子量
hmda 分子量HMDA（Hydroxymethyl Dinitramine）是一种具有较高分子量的化合物。
它的分子量是指该化合物中所有原子的质量之和。
分子量是一个重要的物理化学性质，它可以用来确定化合物的相对质量和化学反应中的摩尔比例。
HMDA的分子量为224.095 g/mol。
这个数值可以通过化学实验或计算得出。
分子量的计算方法是将化合物中每个原子的相对原子质量相加。
例如，HMDA由一种碳原子、四种氢原子、一种氧原子和一种氮原子组成，它们的相对原子质量分别为12.01 g/mol、1.008 g/mol、16.00 g/mol和14.01 g/mol。
将它们相加，得到HMDA 的分子量。
HMDA的分子量对于研究和应用该化合物具有重要意义。
首先，它可以用于确定HMDA的相对质量。
在实验室中，研究人员可以通过测量HMDA样品的质量，并将其与分子量进行比较，以确定样品中HMDA的含量。
这对于确保实验的准确性和可重复性非常重要。
HMDA的分子量可以用于计算化学反应中的摩尔比例。
在化学反应中，不同物质之间的摩尔比例对于反应的进行和产物的生成至关重要。
通过知道反应物的分子量，可以计算出它们之间的摩尔比例，并据此设计和优化化学反应的条件。
HMDA的分子量还可以用于确定其在物理和化学性质方面的相关参数。
例如，它可以用于计算HMDA的密度、沸点、熔点等物理性质。
这些性质对于了解和应用HMDA非常重要，因为它们直接影响到该化合物在实际应用中的性能和稳定性。
除了分子量，HMDA还具有其他一些重要的物理化学性质。
例如，它是一种无色结晶性固体，具有高度的热稳定性和爆炸性。
由于其爆炸性质，HMDA被广泛应用于火箭推进剂、炸药和其他爆炸物的制备中。
同时，HMDA也具有一定的毒性，需要在使用和处理时采取相应的安全措施。
HMDA是一种具有较高分子量的化合物。
它的分子量可以通过计算每个原子的相对原子质量之和来确定。
分子量对于研究和应用HMDA具有重要意义，它可以用于确定化合物的相对质量、计算摩尔比例以及确定物理和化学性质。
HPLC法测定复方苦参注射液中5-羟甲基糠醛的含量
HPLC法测定复方苦参注射液中5-羟甲基糠醛的含量
段秀梅;张亚锰;王鹏飞;毕小凤;海丽娜;秦文杰
【期刊名称】《中国民族民间医药》
【年(卷),期】2022(31)5
【摘要】目的:建立复方苦参注射液中5-羟甲基糠醛(5-HMF)含量的测定方法。
方法:采用高效液相色谱法,色谱柱为Waters XSelect CSH TM C 18(250 mm×4.6 mm,5μm),流动相为甲醇-0.05%甲酸水溶液梯度洗脱,流速为0.8 mL·min^(-1),检测波长为284 nm,柱温20℃,进样量10μL。
结果:5-HMF质量浓度在
0.022~1.11μg(r=0.9998)范围内质量呈良好线性关系。
5-HMF平均回收率为95.6%(RSD=6.70%)(n=9)。
结论:本研究方法重复性好,准确度高,可用于复方苦参
注射液中5-HMF的含量测定。
【总页数】3页(P65-67)
【作者】段秀梅;张亚锰;王鹏飞;毕小凤;海丽娜;秦文杰
【作者单位】北京振东光明药物研究院
【正文语种】中文
【中图分类】R284.1
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