细胞蜡块制作流程及免疫组化运用

细胞蜡块制作流程及免疫组化运用
1.细胞蜡块制作是一种常见的实验技术。

Cell block preparation is a common laboratory technique.
2.首先，将收集到的细胞沉淀在离心管里。

First, the collected cells are centrifuged in a centrifuge tube.
3.接着，将细胞沉淀固定在载玻片上。

Next, the cell pellet is fixed on a slide.
4.固定后，将载玻片放入甲醇中进行脱水。

After fixation, the slide is dehydrated in methanol.
5.然后，用蜡进行浸渍和包埋。

Then, the cells are infiltrated and embedded in paraffin wax.
6.最后，切割成细胞蜡块。

Finally, the wax block is sectioned.
7.细胞蜡块可以用于免疫组化实验。

The cell block can be used for immunohistochemistry.
8.免疫组化是一种检测蛋白表达的技术。

Immunohistochemistry is a technique for detecting protein expression.
9.通过免疫组化，可以确定细胞中特定蛋白的分布和定位。

Immunohistochemistry can determine the distribution and localization of specific proteins in the cells.
10.首先，细胞蜡块被切成非常薄的切片。

First, the cell block is sectioned into very thin slices.
11.然后，切片被放置在载玻片上。

Then, the slices are placed on slides.
12.接下来，切片被处理以暴露蛋白。

Next, the slices are treated to expose the proteins.
13.然后，蛋白被结合上特定的抗体。

Then, the proteins are bound to specific antibodies.
14.最后，使用染色技术来可视化蛋白的表达。

Finally, staining techniques are used to visualize the expression of the proteins.
15.免疫组化可以帮助医生诊断疾病。

Immunohistochemistry can help physicians diagnose diseases.
16.该技术还可以用于研究蛋白在不同情况下的表达。

The technique can also be used to study protein expression in different conditions.
17.免疫组化在癌症诊断中有广泛的应用。

Immunohistochemistry is widely used in cancer diagnosis.
18.通过观察细胞蜡块的免疫组化结果，可以判断肿瘤类型。

By observing the immunohistochemistry results of the cell block, the type of tumor can be determined.
19.有些特定的抗体对于癌症的诊断很关键。

Some specific antibodies are crucial for cancer diagnosis.
20.浓缩蛋白可用于免疫组化检测。

Concentrated protein can be used for immunohistochemical detection.
21.免疫组化技术需要专业的操作和仪器。

Immunohistochemistry techniques require professional operation and equipment.
22.免疫组化结果通常需要在显微镜下观察。

Immunohistochemistry results are usually observed under a microscope.
23.一些特殊的免疫组化实验需要特定的条件和试剂。

Some special immunohistochemistry experiments require specific conditions and reagents.
24.免疫组化对于研究细胞生物学和生物医学具有重要意义。

Immunohistochemistry is of great significance for studying cell biology and biomedicine.
25.免疫组化的结果可以用于发表学术论文。

The results of immunohistochemistry can be used for publishing academic papers.
26.细胞蜡块的制备需要严格的操作和清洁。

The preparation of cell blocks requires strict operation and cleanliness.
27.免疫组化实验需要小心操作以避免污染。

Immunohistochemistry experiments need to be carefully conducted to avoid contamination.
28.免疫组化的结果需要进行定量或定性分析。

The results of immunohistochemistry need to undergo quantitative or qualitative analysis.
29.一些自动化设备可以帮助进行免疫组化实验。

Some automated equipment can assist in conducting immunohistochemistry experiments.
30.免疫组化是病理学检查的重要手段。

Immunohistochemistry is an important tool for pathology examination.
31.细胞蜡块制作和免疫组化技术在临床诊断中有着重要的作用。

Cell block preparation and immunohistochemistry techniques play an important role in clinical diagnosis.
32.免疫组化是一项复杂而精细的技术。

Immunohistochemistry is a complex and delicate technique.
33.细胞蜡块制备和免疫组化的成功需要科学家的细心和耐心。

The success of cell block preparation and immunohistochemistry requires the carefulness and patience of scientists.
34.免疫组化结果的解读需要经验丰富的专家。

Interpreting the results of immunohistochemistry requires experienced experts.
35.一些特殊的抗体需要冷藏保存以保持活性。

Some special antibodies need to be stored in a refrigerated condition to maintain their activity.
36.细胞蜡块的制备过程中需要避免氧化和水解现象。

Oxidation and hydrolysis should be avoided during the preparation of cell blocks.
37.免疫组化实验需要进行阳性和阴性对照以确保结果准确。

Immunohistochemistry experiments need to include positive and negative controls to ensure accurate results.
38.免疫组化的结果可能受到细胞蜡块制备过程的影响。

The results of immunohistochemistry may be affected by the process of cell block preparation.
39.一些抗体需要特殊的预处理才能发挥最佳效果。

Some antibodies require special pretreatment to achieve optimal results.
40.免疫组化的结果需要进行标准化和验证。

The results of immunohistochemistry need to be standardized and validated.
41.许多研究课题都需要运用细胞蜡块制作和免疫组化技术。

Many research topics require the use of cell block preparation and immunohistochemistry techniques.
42.免疫组化实验需要对实验条件进行严格控制。

Immunohistochemistry experiments require strict control of experimental conditions.
43.透明的载玻片可以帮助观察免疫组化结果。

Transparent slides can help observe the results of immunohistochemistry.
44.免疫组化的结果还可以在数字化平台上进行分析。

The results of immunohistochemistry can also be analyzed on digital platforms.
45.医学学术期刊对免疫组化实验的结果有严格的要求。

Medical academic journals have strict requirements for the results of immunohistochemistry experiments.
46.免疫组化技术的进步推动了疾病诊断方法的发展。

The advancement of immunohistochemistry technology has promoted the development of disease diagnosis methods.
47.影响免疫组化结果的因素有很多。

There are many factors that can affect the results of immunohistochemistry.
48.免疫组化是研究细胞分子机制的重要手段。

Immunohistochemistry is an important tool for studying cellular molecular mechanisms.
49.研究中常常需要对细胞蜡块进行多次免疫组化实验。

Multiple immunohistochemistry experiments on cell blocks are often needed in research.
50.免疫组化实验在临床诊断中有着不可替代的作用。

Immunohistochemistry experiments play an irreplaceable role in clinical diagnosis.
51.用适当的控制可以保证免疫组化的结果准确性。

Appropriate controls can ensure the accuracy of immunohistochemistry results.
52.免疫组化技术的发展为医学研究提供了重要的工具。

The development of immunohistochemistry technology has provided important tools for medical research.
53.免疫组化在癌症治疗中也有着重要的应用。

Immunohistochemistry also has important applications in cancer treatment.。