肝细胞癌患者外周血甲胎蛋白mRNA的检测及意义

肝细胞癌患者外周血甲胎蛋白mRNA的检测及意义
曹骥;覃柳亮;苏建家;李瑗;杨南武;谢伟敏;班克臣;欧超;莫钦国
【期刊名称】《癌症（英文版）》
【年(卷),期】2001(020)001
【摘要】Objective： The current study was designed to search into the detection of micrometastasis in peripheral blood in the patients with hepatocellular carcinoma(HCC)and its significance.Method： Peripheral blood samples were obtained from 65 patients with hepatocellular carcinoma, 21 non-HCC malignant tumors, 22 chronic hepatitis B or cirrhosis, and 21 cases of normal healthy volunteers. For identifying hepatocellular carcinoma cells in peripheral blood, the authors detected liver-specific alpha-fetoprotein(AFP) mRNA from total RNA extracted from whole blood by nested reverse transcription-polymerase chain
reaction(Nested-RT-PCR). Results： AFP-mRNA was not detected in the normal healthy volunteers and the patient with non-HCC malignant tumors. The presence of AFP-mRNA in the patients with HCC
(67.7％ ,44/65) was higher than those with chronic hepatitis B or cirrhosis ( 9.1％ ,2/22,P< 0.01). The detective rate of AFP-mRNA in the blood seemed to be correlated with the clinical stages of HCC, the presence of extrahepatic metastasis, and portal vein thrombosis. The positive AFP-mRNA in peripheral blood after treatment was correlated with the prognosis of the patients with HCC. AFP-mRNA was detected in 7 of 12
(58.3％) HCC patients with low level of serum AFP (< 25 μg/L). Conclusions：The presence of AFP-mRNA in peripheral blood may be an indicator of malignant or benign hepatocytes, which might predicate hematogenous spreading metastasis of tumor cells in the patients with HCC, it might be a valuable marker for predicting metastasis and recurrence of HCC, and it might play a supplementary role in the diagnosis of HCC with negative or low level of serum AFP.%目的：探讨肝细胞癌患者外周血中微小转移及其临床意义。

方法：用巢式逆转录聚合酶链反应(Nested-RT-PCR)技术检测65例肝细胞癌、21例非肝癌的恶性肿瘤、22例慢性乙型肝炎或肝硬化患者及21例健康志愿者外
周静脉血中的甲胎蛋白(AFP)mRNA。

结果：AFP mRNA在健康志愿者、非肝癌恶性肿瘤患者外周血中均为阴性；在肝细胞癌组外周血中AFP mRNA的检出率(67.7％，44/65)明显高于慢性乙型肝炎或肝硬化组(9.1％，2/22，P<0.01)。

AFP-mRNA检出率与临床分期、门静脉癌栓、肝外转移显著相关。

经1年的随访发现治疗后外周血AFP-mRNA阳性与肝癌患者预后有显著相关。

在12例血清AFP<25μg/L的肝癌病人血中，7例(58.3％)可检出AFP-mRNA。

结论：AFP-mRNA可作为血循环中有肝癌细胞或肝细胞的标志物，在肝癌患者，阳性预示有
血源性转移的可能，可作为预测肝癌复发、转移的参考指标；并且AFP mRNA
对血清AFP阴性或低值的肝癌患者能起到补充诊断作用。

【总页数】4页(P53-56)
【作者】曹骥;覃柳亮;苏建家;李瑗;杨南武;谢伟敏;班克臣;欧超;莫钦国
【作者单位】广西肿瘤防治研究所病理研究室,;广西肿瘤防治研究所病理研究室,;广西肿瘤防治研究所病理研究室,;广西肿瘤防治研究所病理研究室,;广西肿瘤防治研究
所病理研究室,;广西肿瘤防治研究所病理研究室,;广西肿瘤防治研究所病理研究室,;广西肿瘤防治研究所病理研究室,;广西肿瘤防治研究所病理研究室,
【正文语种】中文
【中图分类】R735.7
因版权原因，仅展示原文概要，查看原文内容请购买。