口腔生物学：从细胞培养到科研设计
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Story-2 炎症及低氧调控SDF-1-CXCR4的表达
ELISA analysis of SDF-1α levels in DPCs. Normal DPCs expressed SDF-1α constitutively. After being stimulated with LPS, SDF-1α production significantly reduced.
Journal of Endodontics, Volume 38, Issue 6, 2012, 791–795
Journal of Endodontics, Volume 38, Issue 6, 2012, 791–795
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2010 2010
2012
2012
wk.baidu.com
2015
？ SDF-1-CXCR4------In vivo-------In vitro--------SP cells---------stem cells---------CXCR4+-------------
Journal of Endodontics, Volume 34, Issue 11, 2008, 1351–1354
The expression of SDF-1/CXCR4 in normal human dental pulp.
Journal of Endodontics, Volume 34, Issue 11, 2008, 1351–1354
Journal of Endodontics, Volume 36, Issue 9, 2010, 1499–1503
Story-3 低氧诱导SP牙髓干细胞的分化
Determination of SP phenotype in human DPCs under different culture conditions by flow cytometry.
Critical Thinking --从细胞培养到科研设计
Critical thinking
▪ Criticize，批判式思考 ▪ 一种思维方式 ▪ 辩证的、逻辑的、批判的思考 ▪ “脑洞大开”、“brain storm” ▪ 专业兴趣、 职业发展、人生方向
科研设计 research design
Journal of Endodontics, Volume 36, Issue 3, 2010, 453–458
Ischemic culture condition enhanced ABCG2 and OCT4 immunofluorescence staining in human DPCs.
Journal of Endodontics, Volume 36, Issue 9, 2010, 1499–1503
The expression levels of the mRNAs encoding HIF-1α, SDF-1α, and CXCR4 in hypoxic DPCs relative to normoxic DPCs as determined by quantitative RT-PCR. The HIF-1α and CXCR4 expression level increased, but SDF-1α expression decreased in the 24-hour culture period.
Journal of Endodontics, Volume 36, Issue 3, 2010, 453–458
Story-4 SP牙髓干细胞具有自我更新和多向分化潜能
The SP and MP cells from human dental pulp and their CFU in 35-mm plates stained by Giemsa. (A) SP cells from human dental pulp and (B) MP cells from human dental pulp (second passage). (A and B) Scale bars = 100 μm. (C) CFU of SP cells (5.3% ± 0.8%)...
ideas
Clinical problems
mimic
LPS+ Hypoxia
Multidifferentiation
总结 conclusion
▪ 科研启蒙课 ▪ 加强思考 ▪ 培养思维
▪ 勿忘初心 方得始终
Journal of Endodontics, Volume 38, Issue 11, 2012, 1489–1497
Journal of Endodontics, Volume 38, Issue 11, 2012, 1489–1497
Story-5 体外筛选CXCR4阳性牙髓细胞
Immunophenotype analysis of CXCR4+ DPCs, CXCR4− DPCs, and non-sorted DPCs by flow cytometry.
▪ 细胞培养等只是研究手段 ▪ 真正需要的是--------idea ▪ 创新的、逻辑的、有效的思考 ▪ 发现问题-论证问题-解决问题 ▪ 从模仿到应用再到超越
Story-1 SDF-1-CXCR4在炎症牙髓组织中发现
The expression of SDF-1/CXCR4 in inflamed human dental pulp.
Journal of Endodontics, Volume 36, Issue 9, 2010, 1499–1503
Western blotting results of CXCR4 production in DPCs; 1 μg/mL LPS increased the expression of CXCR4 in DPCs after 48 hours of treatment; the other two concentrations did not affect CXCR4 production.