METHOD FOR ROBUST CONTROL OF GENE EXPRESSION
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专利名称：METHOD FOR ROBUST CONTROL OF GENE
EXPRESSION
发明人：Michael J. Flynn,Michael B. Elowitz,Acacia
Hori,Viviana Gradinaru
申请号：US17100857
申请日：20201121
公开号：US20210171582A1
公开日：
20210610
专利内容由知识产权出版社提供
专利附图：
摘要：Disclosed herein include methods, compositions, and kits suitable for robust and tunable control of payload gene expression. Some embodiments provide rationally
designed circuits, including miRNA-level and/or protein-level incoherent feed-forward loop circuits, that maintain the expression of a payload at an efficacious level. The circuit can comprise a promoter operably linked to a polynucleotide encoding a fusion protein comprising a payload protein, a protease, and one or more self-cleaving peptide sequences. The payload protein can comprise a degron and a cut site the protease is capable of cutting to expose the degron. The circuit can comprise a promoter operably linked to a polynucleotide comprising a payload gene, a silencer effector cassette, and one or more silencer effector binding sequences.
申请人：California Institute of Technology
地址：Pasadena CA US
国籍：US
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