一株棉花黄萎病拮抗芽孢细菌的分离鉴定及活性检测

一株棉花黄萎病拮抗芽孢细菌的分离鉴定及活性检测
张冬冬;李术娜;郭晓军;朱宝成
【期刊名称】《棉花学报》
【年(卷),期】2012(024)004
【摘要】采用改良的琼脂平板扩散法,通过初筛和复筛,得到一株对棉花黄萎病病原菌大丽轮枝菌(Verticillium dahliae)具有较强的抑菌活性的拮抗细菌Z-5菌株.通过对该菌株的形态特征观察、生理生化鉴定和16S rDNA 的序列分析,确定拮抗细菌Z-5菌株为Bacillus malacitensis.盆栽实验表明Z-5菌株对大丽轮枝菌的防治效果达到76.05％,对棉花黄萎病具有明显的防治作用.%A bacterial strain called Z-5 that had strong inhibition capability against Verticillium dahliae V-190 was obtained via preliminary and secondary screening with the improved agar plate diffusion method. Characterized by morphological and cultural features observation, physiological and biochemical experiments, and 16S rDNA sequence analysis, the Z-5 strain was initially identified as Bacillus malacitensis. Control of cotton Verticillium wilt by this antagonistic bacterium was assessed via potted plants. Results showed that the control efficacy of the antagonistic Z-5 strain on cotton Verticillium wilt was 76.05%. Preliminary results demonstrated that the antagonistic Z-5 bacterial strain could significantly inhibit the growth of V. dahliae.
【总页数】5页(P358-362)
【作者】张冬冬;李术娜;郭晓军;朱宝成
【作者单位】河北农业大学生命科学学院,河北保定 071001;河北农业大学生命科学学院,河北保定 071001;河北农业大学生命科学学院,河北保定 071001;河北农业大学生命科学学院,河北保定 071001
【正文语种】中文
【中图分类】S435.621
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