东北红豆杉成分含量研究报告

东北红豆杉有效成分含量

研究报告

东北红豆杉（Taxus cuspidata Sieb. et. Zucc.）是红豆杉科（Taxaceae）红豆杉属植物，是我国东北分布的珍贵第三纪孑遗树种、国家I级重点保护的野生濒危植物种类。紫杉醇（paclitaxel，商品名taxol）及三尖杉宁碱（cephalomannine）均为红豆杉属植物中含有的二萜类化合物，具广谱抗肿瘤活性和独特的抗癌作用机理。

本实验探讨了不同部位、不同生长时期东北红豆杉枝叶中的紫杉醇和三尖杉宁碱含量的分布规律，结果表明多数树冠部位的当年生枝叶中两种物质的含量低于两年生和三年生枝叶，而两年生和三年生枝叶中两种物质含量差异不显著；阳面树冠的3个层次的两种物质含量无明显差异，阳面的含量稍高于阴面。天然东北红豆杉当年生枝叶中紫杉醇含量在休眠期达到最高，花期和果期含量无太大差异；三尖杉宁碱含量在休眠期也最高，部分植株的当年生枝叶中三尖杉宁碱含量为花期低于果期，但有个别植株中二者含量差异不显著；每个植株个体之间紫杉醇和三尖杉宁碱含量均差异显著。

Variation in Paclitaxel and Cephalomannine contents

in nature Japanese yew

Abstract

The Japanese yew (Taxus cuspidata Sieb. et. Zucc.) is a survivor tertiary species and national level endangered species which belongs to Taxaceae.

Paclitaxel and cephalomannine are both diterpenoid in Taxus with special anticancer mechanism and antitumous activity.

This experiment have determined the paclitaxel and cephalomannine contents in branches and leaves of Japanese yew in different parts and periods. The results indicated that most parts of paclitaxel and cephalomannine contents in branches and leaves of current year were lower than those of biennial and perennial, but paclitaxel and cephalomannine contents in branches and leaves had no conspicuous difference between biennial and perennial; there were no conspicuous difference in three hierarchical，but the contents of the two materials in sunny side were slightly higher than the overcast side. Paclitaxel contents of current branches and leaves were highest during dormancy stage, but it had no signficant difference between flower season and fruit season. The contents of cephalomannine was highest during dormancy stage too, and the cephalomannine contents in flower season were lower than fruit season of parts of samples trees, but some had no significant difference, individual variation was significant.

Key words：Taxus cuspidata；paclitaxel；cephalomannine；ultrasonic extraction method；HPLC

1 绪论 (1)

1.1 植物次生代谢 (1)

1.2 东北红豆杉 (1)

1.2.1 东北红豆杉生物学特征 (1)

1.2.2 东北红豆杉资源的利用价值 (2)

1.2.3 东北红豆杉资源及现状 (2)

1.3 紫杉醇和三尖杉宁碱 (3)

1.3.1 东北红豆杉枝叶中的化学成分 (3)

1.3.2 紫杉醇 (3)

1.3.3 三尖杉宁碱 (4)

1.3.4 研究进展 (5)

1.4 立题依据 (6)

2 实验材料及方法 (7)

2.1 实验材料 (7)

2.2 仪器与试剂 (7)

2.2.1仪器.............. ........................................................................................... . (7)

2.2.2试剂 (7)

2.3 实验方法 (7)

2.3.1 样品制备 (7)

2.3.2 色谱条件 (8)

2.3.3 标准溶液配制 (8)

2.3.4 标准曲线绘制 (8)

3 结果与分析 (9)

3.1 天然东北红豆杉不同树冠部位枝叶中紫杉醇及三尖杉宁碱的含量 (9)

3.1.1 天然东北红豆杉不同树冠部位叶中紫杉醇及三尖杉宁碱的含量 (9)

3.1.2 天然东北红豆杉不同树冠部位枝中紫杉醇及三尖杉宁碱的含量 (10)

3.2 天然东北红豆杉当年生枝叶中紫杉醇及三尖杉宁碱含量的生长时期变化规律 (10)

3.2.1 当年生叶中紫杉醇及三尖杉宁碱含量的生长时期变化规律 (10)