神经解剖方法学ppt课件

Glees 、Nauta、Fink-Heimer
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1.1. Golgi ‘s method
Camello Golgi (1843-1926 Italian). He developed a way of staining neurons with silver salts that revealed their entire structure under the microscope(1873).
A： Anterograde tracing B： Retrograde tracing
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Retrograde tracing with HRP
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2.1.2. Anterograde Tracing with PHA-L
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2.1.3. Tracing with fluorescence
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Gless method
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Nauta .stain
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Fink-Heimer method
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Fink-He.imer 法
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1.7. Other methods
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Sec cell Nerve Fibers
H-E stain
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Structure of the neuron
Ramon y Cajal (1852~1934)
One of the Cajal’s drawings
of .brain circuitry
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Neuron
Dendrite Nerve Fibers
Cajal’s method
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Neurofibrils Neuroglia
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Cajal’ method
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1.3. Nissl’s method
Franz Nissl (18601919 ,Germany), The Nissl’s method shows cell bodies and proximal dendrites (1892).
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神经元的结构
Golgi-stained neurons
Camillo Golgi (1843~1926)
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Golgi ‘method
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1.2. Cajal’s method
Ramon y Cajal (1852-1943,Spanish.). Using Golgi’s technique, he was able to stain individual cells, thus showing that nervous tissue is not one continuous web but a network of discrete cells. R.Cajal developed some of the key concepts and much of the early evidence for the neuron doctrine (1903).
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Spinal ganglion NucleoLus
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H-E stain
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Osmium stain
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Fast Blue stain
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Lux-Fast blue methoud
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2. Modern Experimental Neuroanatomy
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2.1. Neuronal tract tracing
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Weige.rt stain
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Weiger.t stain
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1.5. Marchi method
Vittario Marchi (1851-1908, Italian).
Marchi's method (developed and described about 1890) has yielded much information about human neuroanatomy, comparable to that obtained from staining degenerated tracts in lab animals, prior to the development of anterograde
Retrograde tracing Anterograde tracing
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2.1.1. Retrograde tracing with HRP
HRP ( horseradish peroxidase) The characteristic of HRP RZ(纯度值）
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Tracing with HRP
1.Traditional
Experimental Neuroanatomy
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Golgi: Camello Golgi (1843-1926), Cajal: Ramon y Cajal (1852-1943) Nissl: Franz Nissl (1860-1919) Weigert: Karl Weigert (1843-1904), Marchi: Vittario Marchi (1851-1908)
Nissl stain
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1.4. Weigert’s method
Karl Weigert (1843-1904, Germany). Weigert stain for myelinated fibers reveals the patterm of axonal distribution (1884).
and retrograde tracing experiments.
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Marchi method
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1.6. Nauta and Fink-Heimer’s method
The Nauta method, introduced in the 1950s, and the Fink-Heimer method, introduced in 1967, use silver impregnation of degenerating axons.