癌基因与抑癌基因ppt课件

1979, H. Varmus and J. M. Bishop found that src is carcinogenesis in RSV, but also found that the homologs of src exist in animal cells commonly if the cDNA of src is hybridized with other genome DNA. Result: src stem from normal cell genome. ----- 1989 Nobel
src
LTR
regulating and initiating transcription
the core protein of virus
reverse transcriptase and Integrase
Outer membrane protein
Tyrosine kinase
the structure of Rous sarcoma virus
二、Virus oncogenes
the oncogene existing in virus genome which does not encode the protein of virurs and is not helpful to replication but do make host cell Continuous proliferation
Negative regulation tumor suppressor gene
cells
oncogene Positive regulation
products
Growth factor
• The regulation of gene expression and cell proliferation, differentiation is involved in the mechanism of oncogenes and tumor suppressor
• Oncogene, antioncogene, tumor metastasis gene, cell apoptosis, DNA damage and repair, telomerase, microRNA
Relationships of oncogene, tumor suppressor gene and growth factors
Rous Peyton
Rous 肉瘤
பைடு நூலகம்
1970,Temin and Batimore, reverse transcriptase，1975 Nobel 1971, Duesberg, the genome of RSV
Normal virus gene
oncogene
5’
LTR gag
pol
3’
env
All of genes which encode for growth factors, growth factor receptors, signal molecules and transcription factors related to growth.
The structural comparison of c-src and v-src
（一）the traits of cellular oncogene
Universality ：exists in biological widely；
conservatism ：The gene sequence was highly homologous in the process of evolution
Voiceover : that is why such RSV retrovirus induce host cell carcinogenesis.
三、cellular oncogene
the oncogenes existing in normal genome which are in quiescence or inactive state such as c-myc、c-ras、csrc。Under normal condition，these genes are in quiescence or low expression state and not only no harmful to cells but also helpful to stain normal function to cells.
Section I Oncogene
• Concept of oncogene • Viral oncongene • Cellular oncogene • The products of oncongene and
its fuction
一、the concept of oncogene
Oncogene：the genes in cells is responsible of controlling cell growth, proliferation and differentiation. They will cause cellular canceration when their structure or expression turns abnormal.
OVERVIEW
• Many genes and factors outside of genes is involved in carcinogenesis. The accumulation of many genetic changes cause cancer other than mutations of a single gene by causing disorders of cell growth and differentiation, the cell proliferation out of control.
Importance ：the expression protein regulate cell proliferation, differentiation and apoptosis precisely