各种激发光和吸收光波长
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各种激发光和吸收光波长
现在发现一个更全的有关激发光和发射光的波长图谱。这对CONFOCAL去做多重染色时是必不可少的参考资料。
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Excitation and Emission Wavelengths of Fluorophores
Tavi\'s \"FluoroTable\": Common fluorophores
(from )
Fluorophore Absorption Emission Other info
1,5 IAEDANS 336490
1,8-ANS372480
4-Methylumbelliferone385502
5-carboxy-2,7-dichlorofluorescein504529
5-Carboxyfluorescein (5-FAM)492518
5-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH
5-Carboxytetramethylrhodamine (5-TAMRA)542568
5-FAM (5-Carboxyfluorescein)492518
5-HAT (Hydroxy Tryptamine)370-415520-540
5-Hydroxy Tryptamine (HAT)370-415520-540
5-ROX (carboxy-X-rhodamine)578
567604 591
5-TAMRA (5-Carboxytetramethylrhodamine)548
542552 568
6-Carboxyrhodamine 6G518543
6-CR 6G518543
6-JOE520548
7-Amino-4-methylcoumarin351430
7-Aminoactinomycin D (7-AAD)546647
7-Hydroxy-4-methylcoumarin360449, 455
9-Amino-6-chloro-2-methoxyacridine412, 430471, 474
ABQ344445
Acid Fuchsin540630
ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474
Acridine Orange + DNA502526
Acridine Orange + RNA460650
Acridine Orange, both DNA & RNA440-480520-650
Acridine Red455-600560-680
Acridine Yellow470550
Acriflavin436520
Acriflavin Feulgen SITSA355-425460
Aequorin (Photoprotein)466Photoprotein AFPs -AutoFluorescent Protein -(Quantum
Biotechnologies) see sgGFP, sgBFP
Alexa Fluor 350346
342442 441
Alexa Fluor 430431540 Alexa Fluor 488495, 492519, 520 Alexa Fluor 532531, 532553, 554 Alexa Fluor 546556, 557572, 573 Alexa Fluor 568577, 578603 Alexa Fluor 594590, 594617, 618 Alexa Fluor 633632650 Alexa Fluor 647647666 Alexa Fluor 660668698 Alexa Fluor 680679702
Alizarin Complexon530-560,
580580, 624-645
Alizarin Red530-560580 Allophycocyanin (APC)630, 645655, 660 AMC, AMCA-S345445
AMCA (Aminomethylcoumarin)345
347425 444
AMCA-X353442 Aminoactinomycin D555655
Aminocoumarin346
350442 445
Aminomethylcoumarin (AMCA)345
347425 444
Anilin Blue600
Anthrocyl stearate360-381446
APC (Allophycocyanin)630, 645655, 660
APC-Cy7625-650755
APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+ APTS424505
Astrazon Brilliant Red 4G500585
Astrazon Orange R470540
Astrazon Red 6B520595
Astrazon Yellow 7 GLL450480
Atabrine436490
ATTO-TAG CBQCA465560
ATTO-TAG FQ486591
Auramine460550
Aurophosphine G450580
Aurophosphine450-490515
BAO 9(Bisaminophenyloxadiazole)365395
BCECF (high pH)492, 503520,528
BCECF (low pH)482520
Berberine Sulphate430550
Beta Lactamase409447, 520