常用酿酒酵母菌株基因型教程文件

常用酿酒酵母菌株基

因型

Commonly used strains

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▪van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains

▪Winzeler et al. (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains

S288C

Genotype:MATαSUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6

Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically.

The S288C genome was recently resequenced at the Sanger Institute.

References:Mortimer and Johnston (1986) Genetics 113:35-43.

BY4743

Genotype:MAT a/αhis3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0

Notes: Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant of MIP1 which increases petite frequency. See Brachmann et al. reference for details.

References:Brachmann et al. (1998) Yeast 14:115-32.

FY4

Genotype:MAT a

Notes: Derived from S288C.

References:Winston et al. (1995) Yeast 11:53-55.

FY1679

Genotype:MAT a/αura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 GAL2/GAL

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References:Winston et al. (1995) Yeast 11:53-55.

AB972

Genotype:MATα X2180-1B trp10 [rho 0]

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.

References:Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.

A364A

Genotype:MAT a ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIO

Notes: Used in the systematic sequencing project, the sequence stored in SGD.

References:Hartwell (1967) J. Bacteriol. 93:1662-1670.

XJ24-24a

Genotype:MAT a ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2

Notes: Derived from, but not isogenic to, S288C

References:Strathern et al. (1979) Cell 18:309-319

DC5

Genotype:MAT a leu2-3,112 his3-11,15 can1-11

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References:Broach et al. (1979) Gene 8:121-133

X2180-1A

Genotype:MAT a SUC2 mal mel gal2 CUP1

Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180