功能化氧化石墨烯的细胞相容性

[Article]

物理化学学报(Wuli Huaxue Xuebao )

Acta Phys.⁃Chim.Sin .2012,28(6),1520-1524

June

Received:November 25,2011;Revised:March 11,2012;Published on Web:March 13,2012.∗

Corresponding authors.YANG Rong,Email:yangr@;Tel:+86-10-82545616.HENG Cheng-Lin,Email:hengcl@.The project was supported by the National Natural Science Foundation of China (20911130229,21073047)and Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences,China (KJCX2.YW.M15).

国家自然科学基金(20911130229,21073047)和中国科学院知识创新工程重要方向项目(KJCX2.YW.M15)资助

ⒸEditorial office of Acta Physico ⁃Chimica Sinica

doi:10.3866/PKU.WHXB 201203131

功能化氧化石墨烯的细胞相容性

张晓1,2

杨蓉2,*王琛2衡成林1,*

(1北京理工大学物理学院,教育部簇科学重点实验室,北京100081;

2

国家纳米科学中心,中国科学院纳米生物效应与安全性重点实验室,北京100190)

摘要:采用改进的Hummers 方法制备了纳米尺度的氧化石墨烯.对氧化石墨烯的表面进行羧基化,并连接上

聚乙二醇(PEG)使其在细胞培养液中可溶并能稳定保存.采用透射电镜(TEM)、傅里叶变换红外(FTIR)光谱和zeta 电位测量等对修饰后的氧化石墨烯的结构和功能进行了表征.体外细胞实验表明PEG 修饰的氧化石墨烯在水中具有良好的可溶性,对A549细胞没有明显的毒性,在生物医药领域具有潜在的应用价值.关键词:

氧化石墨烯;纳米材料;生物相容性;表面功能化

中图分类号:

O645

Cell Biocompatibility of Functionalized Graphene Oxide

ZHANG Xiao 1,2

YANG Rong 2,*

WANG Chen 2

HENG Cheng-Lin 1,*

(1Key Laboratory of Cluster Science of Ministry of Education,School of Physics,Beijing Institute of Technology,Beijing 100081,P .R.China ;2Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,Chinese Academy of Sciences,

National Center for Nanoscience and Technology,Beijing 100190,P .R.China )

Abstract:We report on synthesis of nanoscale graphene oxide (NGO)by modified Hummers ’method.Synthesized NGO particles were surface functionalized by attaching carboxylic acid and polyethylene glycol groups to render them soluble in cell culture medium.The structures and properties of functionalized NGO were characterized by transmission electron microscopy (TEM),Fourier transform infrared (FTIR)spectroscopy,and zeta potential analyzer.Cell viability studies show that PEG-modified NGO particles are highly soluble and incur almost no cytotoxicity to A549cells,which suggest a great potential for the use of NGO in various biomedical applications.Key Words:Graphene oxide;

Nanomaterials;Biocompatibility;Surface functionalization

1Introduction

Graphene,a single layer of carbon atoms with excellent ther-mal,mechanical,and electrical properties,has attracted consid-erable attention in recent years.1-3Graphene oxide (GO)4-10is one of the most important graphene derivatives which contains aromatic regions randomly interspersed with oxidized aliphatic rings.These oxidized rings containing epoxide (C ―O ―C)and hydroxyl (C ―OH)groups,and the GO sheets terminated with both carbonyl (C ＝O)and carboxylic acid (―COOH)

groups 5-7can provide reactive sites for chemical modification to obtain new derivatives for biology application.8-10It is known that many pharmaceutical ingredients are poorly solu-ble in water.As a result,their clinical applications are seriously influenced.Therefore,finding a nanoscale drug carrier is criti-cal in biology application.Recently,researchers started to ex-plore the ability of GO in attachment and delivery of aromatic,water insoluble drugs.Yang et al.11investigated loading doxo-rubicin hydrochloride,an anticancer drug,on GO sheets,and

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