低分子量的达肝素钠和依诺肝素钠在体积排阻色谱柱的分离

min 10121416182022

nRIU

5000

10000

15000

20000

25000

30000

min 10121416182022

mAU

1

2

3

4

1

8

.

2

1

9

1

6

.

1

3

1

9

.

9

8

UV 234 nm

RI

Columns: Zenix™-100 (3 m, 100 Å, 7.8x300 mm)

Flow Rate:

Mobile phase:

0.5 mL/min

2.84% Na2SO4, pH 5.0

Temperature: 35 o C

Detection: UV 234 nm and RI

Injection volume: 25 µL

Samples: 10 mg/mL Dalteparin in water, pH 6.0, MW 3,000 – 8,000 Da

LMW Heparin-Dalteparin and Enoxaparin Analysis on

Zenix™-100 (7830)

Columns: Zenix ™-100 (3 m, 100 Å, 7.8x300 mm) Flow Rate: Mobile phase: 0.5 mL/min

2.84% Na 2SO 4, pH 5.0 Temperature: 35 o C

Detection:

UV 234 nm and RI Injection volume: 25 µL

Samples: 10 mg/mL Enoxaparin in mobile phase, MW 3,000 – 8,000 Da

Keywords: Size exclusion, Zenix, Zenix-100, low molecular weight heparin, high resolution, Enoxaparin, Dalteparin, Pharmaceuticals