lecture_8_Binding and kinetics_结合与动力学
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From Lecture 5:
!
D
!
],
, how much repressor is bound non-specifically to DNA
bp/genome; 10 bp/site; volume. E.coli 1 µm3)
=
10#4M
10#4M+10-2M
=0.01
Hardly any free repressor; almost all bound to nonspecific DNA!
M for non-operator DNA (non-specific binding)
Figure from: Goodrich, Kugel
information on rates of chemical changes! generic chemical reaction:Figure from: Haynie, Biological Thermodynamics++
Figure from: Haynie, Biological Thermodynamics
Figure from: Haynie, Biological Thermodynamics
!Figure from: Haynie, Biological Thermodynamics
ΔG 0= ΔG off *-ΔG on
A+B
AB
++
++
It takes 0.1 seconds to switch off gene expression in E.coli after
(1 operator sequence in 1 µm3 cell),
(is actually measured to be 1010 M-1s-1) How come this is much faster than diffusion limit???
Searching 100 kb with only 1D sliding would take
?
et al., Science (2001)
A+B
AB
++++ΔG 0= ΔG off *-ΔG on