通用引物列表
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引物名称 M13R M13F
序列(5'-3') CAG GAA ACA GCT ATG ACC TGT AAA ACG ACG GCC AGT
M13F(-47) CGC CAG GGT TTT CCC AGT CAC GAC M13R(-48) AGC GGA TAA CAA TTT CAC ACA GGA M13(-96) SP6 CCC TCA TAG TTA GCG TAA CG ATT TAG GTG ACA CTA TAG
TAT GGC TGA TTA TGA TCA GT AAG AAG GGC AGC ATT CAA AG ATG GAC TAT CAT ATG CTT ACC GTA AGG CGA TTA AGT TGG GTA GAG TTA GCT CAC TCA TTA GGC GAA CGC CAG CAC ATG GAC CTC CGA GAT CTG GAC GAG C GAC GAT AGT CAT GCC CCG CG CTA GCA AAA TAG GCT GTC CC GTT CTG AGG TCA TTA CTG G TGA GCG GAT AAC AAT TTC AC TCA AGC CTC AGA CAG TGG TTC TGA AGC GCA TGA ACT CCT TG
T7 TAA TAC GAC TCA CTA TAG GG T7 terminator TGC TAG TTA TTG CTC AGC GG T3 pGEX-4T5' pGEX-4T3' GLp1 GLp2 RVp3 RVp4 pcDNA3.1 R PinPoint primer pCMV-F ATT AAC CCT CAC TAA AGG GA GGG CTG GCA AGC CAC GTT TGG TG CCG GGA GCT GCA TGT GTC AGA GG TGT ATC TTA TGG TAC TGT AAC TG CTT TAT GTT TTT GGC GTC TTC CA CTA GCA AAA TAG GCT GTC CC GAC GAT AGT CAT GCC CCG CG TAG AAG GCA CAG TCG AGG CGT GAC GCG GTG CAG GGC G TCT AAA AGC TGC GGA ATT GT
pB42ADF: CCA GCC TCT TGC TGA GTG GAG ATG
pEGFP-C3' : PBV220F: U6 2.0rev primer: 3.0rev: S.tag 5'TriplEx2 RVP4 RVP3 pQE30-R pQE30-F pEF-F pDSREDN1-R pDsREDexpressC1-F pCMV5R pCMV5F P5' P3' BAC2 BAC1 3'BD: pTarget.F
pCMV-R TCCAAACTCATCAATGTATC pTRC99CF: TTG CGC CGA CAT CAT AAC pTRC99CR: CTGCGTTCTGATTTAATCTG pCEP-F: EBV-R : pIRES2EGFP.P5’: pIRES2EGFP.P3’: 3'AD: CMV -F S1 S6 5`AOX1 3`AOX1 α-Factor GAL4 AD pACT2-R pB42ADR: pEGFP-N-5 ’: pEGFP-N-3 ’: pEGFP-C-5 ’: AGA GCT CGT TTA GTG AAC CG GTG GTT TGT CCA AAC TCA TC GTA GGC GTG TAC GGT GGG AG AAC GCA CAC CGG CCT TAT TC AGA TGG TGC ACG ATG CAC AG CGC AAA TGG GCG GTA GGC GTG CAA CGT GAA AAA ATT ATT ATT CGC GTA AAT GAA TTT TCT GTA GTA GG GAC TGG TTC CAA TTG ACA AGC GCA AAT GGC ATT CTG ACA TCC TAC TAT TGC CAG CAT TGC TGC TAC CAC TAC AAT GGA TG GTGCACGATGCACAGTTGAA AAG CCG ACA ACC TTG ATT GGA G TGG GAG GTC TAT ATA AGC AGA G CGT CGC CGT CCA GCT CGA CCA G CAT GGT CCT GCT GGA GTT CGT G
pTarget.R(17base ) ACA TCC ACT TTG CCT TTC TC pYes2.R GAL4 BD pAS2-1.R pFlagCMV-F: pFlagCMV-R: pIRES-F: pIRES.R: P1 P2 pLEXA-F pLEXA-R pLNCX-F pLNCX-R pLXSN-F TCG GTT AGA GCG GAT GTG TCA TCG GAA GAG AGT AG AAG AGT TAC TCA AGA ACA AGA A GGT AGG CGT GTA CGG TGG GCA CTG GAG TGG CAA CTT CTT ACT GAC ATC CAC TTT GC CAC TGC ATT CTA GTT GTG GT CCA GGC TTT ACA CTT TAT GC GCG ATT AAG TTG GGT AAC GC CGT CAG CAG AGC TTC ACC AT TAA AAC CTA AGA GTC ACT TT AGC TCG TTT AGT GAA CCG TCA GAT CG ACC TAC AGG TGG GGT CTT TCA TTC CC CTT GAA CCT CCT CGT TCG AC
pLXSN-R GTT GCT GAC TAA TTG AGA TG pSHUTTLE -CMV-F GGT CTA TAT AAG CAG AGG TG
pSHUTTLE -CMV-R GTG GTA TGG CTG ATT ATG ATC AG MalE Primer GGT CGT CAG ACT GTC GAT GAA GCC
PBV220R: CTG CGT TCT GAT TTA ATC TG
TCC CAC AAC GAG GAC TAC AC ATT ATA GAG GAC ACC TAG TC TTC CAA AAT GTC GTA ATA AC TGC GTA CTG CGG TGA TCA AC CTG CAA GGC GAT TAA GTT GG ACG CAC AGA ATC TAG CGC TT AAC CAT CTC GCA AAT AAA TA TAA GAG TCA CTT TAA AAT TTG TAT AC CCA GGA TTT TCC CAG TCA C
序列(5'-3') CAG GAA ACA GCT ATG ACC TGT AAA ACG ACG GCC AGT
M13F(-47) CGC CAG GGT TTT CCC AGT CAC GAC M13R(-48) AGC GGA TAA CAA TTT CAC ACA GGA M13(-96) SP6 CCC TCA TAG TTA GCG TAA CG ATT TAG GTG ACA CTA TAG
TAT GGC TGA TTA TGA TCA GT AAG AAG GGC AGC ATT CAA AG ATG GAC TAT CAT ATG CTT ACC GTA AGG CGA TTA AGT TGG GTA GAG TTA GCT CAC TCA TTA GGC GAA CGC CAG CAC ATG GAC CTC CGA GAT CTG GAC GAG C GAC GAT AGT CAT GCC CCG CG CTA GCA AAA TAG GCT GTC CC GTT CTG AGG TCA TTA CTG G TGA GCG GAT AAC AAT TTC AC TCA AGC CTC AGA CAG TGG TTC TGA AGC GCA TGA ACT CCT TG
T7 TAA TAC GAC TCA CTA TAG GG T7 terminator TGC TAG TTA TTG CTC AGC GG T3 pGEX-4T5' pGEX-4T3' GLp1 GLp2 RVp3 RVp4 pcDNA3.1 R PinPoint primer pCMV-F ATT AAC CCT CAC TAA AGG GA GGG CTG GCA AGC CAC GTT TGG TG CCG GGA GCT GCA TGT GTC AGA GG TGT ATC TTA TGG TAC TGT AAC TG CTT TAT GTT TTT GGC GTC TTC CA CTA GCA AAA TAG GCT GTC CC GAC GAT AGT CAT GCC CCG CG TAG AAG GCA CAG TCG AGG CGT GAC GCG GTG CAG GGC G TCT AAA AGC TGC GGA ATT GT
pB42ADF: CCA GCC TCT TGC TGA GTG GAG ATG
pEGFP-C3' : PBV220F: U6 2.0rev primer: 3.0rev: S.tag 5'TriplEx2 RVP4 RVP3 pQE30-R pQE30-F pEF-F pDSREDN1-R pDsREDexpressC1-F pCMV5R pCMV5F P5' P3' BAC2 BAC1 3'BD: pTarget.F
pCMV-R TCCAAACTCATCAATGTATC pTRC99CF: TTG CGC CGA CAT CAT AAC pTRC99CR: CTGCGTTCTGATTTAATCTG pCEP-F: EBV-R : pIRES2EGFP.P5’: pIRES2EGFP.P3’: 3'AD: CMV -F S1 S6 5`AOX1 3`AOX1 α-Factor GAL4 AD pACT2-R pB42ADR: pEGFP-N-5 ’: pEGFP-N-3 ’: pEGFP-C-5 ’: AGA GCT CGT TTA GTG AAC CG GTG GTT TGT CCA AAC TCA TC GTA GGC GTG TAC GGT GGG AG AAC GCA CAC CGG CCT TAT TC AGA TGG TGC ACG ATG CAC AG CGC AAA TGG GCG GTA GGC GTG CAA CGT GAA AAA ATT ATT ATT CGC GTA AAT GAA TTT TCT GTA GTA GG GAC TGG TTC CAA TTG ACA AGC GCA AAT GGC ATT CTG ACA TCC TAC TAT TGC CAG CAT TGC TGC TAC CAC TAC AAT GGA TG GTGCACGATGCACAGTTGAA AAG CCG ACA ACC TTG ATT GGA G TGG GAG GTC TAT ATA AGC AGA G CGT CGC CGT CCA GCT CGA CCA G CAT GGT CCT GCT GGA GTT CGT G
pTarget.R(17base ) ACA TCC ACT TTG CCT TTC TC pYes2.R GAL4 BD pAS2-1.R pFlagCMV-F: pFlagCMV-R: pIRES-F: pIRES.R: P1 P2 pLEXA-F pLEXA-R pLNCX-F pLNCX-R pLXSN-F TCG GTT AGA GCG GAT GTG TCA TCG GAA GAG AGT AG AAG AGT TAC TCA AGA ACA AGA A GGT AGG CGT GTA CGG TGG GCA CTG GAG TGG CAA CTT CTT ACT GAC ATC CAC TTT GC CAC TGC ATT CTA GTT GTG GT CCA GGC TTT ACA CTT TAT GC GCG ATT AAG TTG GGT AAC GC CGT CAG CAG AGC TTC ACC AT TAA AAC CTA AGA GTC ACT TT AGC TCG TTT AGT GAA CCG TCA GAT CG ACC TAC AGG TGG GGT CTT TCA TTC CC CTT GAA CCT CCT CGT TCG AC
pLXSN-R GTT GCT GAC TAA TTG AGA TG pSHUTTLE -CMV-F GGT CTA TAT AAG CAG AGG TG
pSHUTTLE -CMV-R GTG GTA TGG CTG ATT ATG ATC AG MalE Primer GGT CGT CAG ACT GTC GAT GAA GCC
PBV220R: CTG CGT TCT GAT TTA ATC TG
TCC CAC AAC GAG GAC TAC AC ATT ATA GAG GAC ACC TAG TC TTC CAA AAT GTC GTA ATA AC TGC GTA CTG CGG TGA TCA AC CTG CAA GGC GAT TAA GTT GG ACG CAC AGA ATC TAG CGC TT AAC CAT CTC GCA AAT AAA TA TAA GAG TCA CTT TAA AAT TTG TAT AC CCA GGA TTT TCC CAG TCA C