病原学检查方法
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gravitational sedimentation method自然沉淀法 centrifugal sedimentation method离心沉淀法
larva hatching method:
miracidium hatching method culture method for hookworm larva
The processes of experiments.
Preparing for the next class
Contents: Principles Experiments Medical terms
blood smear thin smear： thick smear：
stainWright stain Giemsa stain
Practice 1
Practicing thin smear, stain and
observing Plasmodium berghei.
取血与涂片
• 薄血膜制片 • 厚血制片
Fresh stool should be examined, or preserved immediately.
How to find parasites in the feces?
Macrography(肉眼观察） Wet mount examination (Direct smear ) Concentration methods Larva hatching methods
How to confirm the density of parasite or worm burden?
Modified Kato-KaBaidu Nhomakorabeaz’s method Stoll’s eggs counting
Practice 2
Self-examining stool yourself! Direct smear with saline Saturated brine flotation
Examining L.donovani from marrow
Aspiration (穿刺检查):
Stool Examination
Significance: Caution:
Collect the stool in a dry, clean, leakproof container. Make sure no urine, water, soil or other material gets in the container.
Stool Examination
Flotation method:
Zinc sulfate centrifugal flotation Saturated brine flotation Sucrose sugar flotation
Stool Examination
Sedimentation method:
Stool Examination
Wet mount method:
Direct smear with saline Iodine staining method（碘染色法）
Stool Examination
Concentration method: modified Kato-Katz’s method sedimentation methods flotation methods
Examining parasite from perianal skin
Parasite
eggs and adults of pinworm eggs of Taenia
Methods
cellophane tape method（透明胶纸法） cotton swab method （棉拭子法）
Label the container with the patient’s name , date and time of collection.
Stool Examination
Before the patient taking medicine the stool specimen should be collected.
Sputum, Urine, Vaginal secretion, Duodenal fluid, Humores, CSF-----
Biopsy and animal inoculation
Summary
Principles of different methods of parasitic diagnosis. ( you should master them.)
Parasitic Pathogen Examination
寄生虫病原检查
Etioligic Diagnosis
Specimen:
Stool, blood, marrow, urine, sputum, humores(体液), secretion and tissue.
Purpose of examination
固定与染色
固定 薄血膜晾干后，甲醇固定
染色 将姬氏染液滴于已固定的薄血
膜上，染色半小时（室温），自 来水冲洗。血片晾干后镜检。
Blood and marrow examination
Examining microfilaria from blood
Time for blood collecting Methods:
Examining pathogen, identifying the species of parasite and the degree of infection.
Blood and marrow examination
Examining plasmodium
the collection of blood time：
larva hatching method:
miracidium hatching method culture method for hookworm larva
The processes of experiments.
Preparing for the next class
Contents: Principles Experiments Medical terms
blood smear thin smear： thick smear：
stainWright stain Giemsa stain
Practice 1
Practicing thin smear, stain and
observing Plasmodium berghei.
取血与涂片
• 薄血膜制片 • 厚血制片
Fresh stool should be examined, or preserved immediately.
How to find parasites in the feces?
Macrography(肉眼观察） Wet mount examination (Direct smear ) Concentration methods Larva hatching methods
How to confirm the density of parasite or worm burden?
Modified Kato-KaBaidu Nhomakorabeaz’s method Stoll’s eggs counting
Practice 2
Self-examining stool yourself! Direct smear with saline Saturated brine flotation
Examining L.donovani from marrow
Aspiration (穿刺检查):
Stool Examination
Significance: Caution:
Collect the stool in a dry, clean, leakproof container. Make sure no urine, water, soil or other material gets in the container.
Stool Examination
Flotation method:
Zinc sulfate centrifugal flotation Saturated brine flotation Sucrose sugar flotation
Stool Examination
Sedimentation method:
Stool Examination
Wet mount method:
Direct smear with saline Iodine staining method（碘染色法）
Stool Examination
Concentration method: modified Kato-Katz’s method sedimentation methods flotation methods
Examining parasite from perianal skin
Parasite
eggs and adults of pinworm eggs of Taenia
Methods
cellophane tape method（透明胶纸法） cotton swab method （棉拭子法）
Label the container with the patient’s name , date and time of collection.
Stool Examination
Before the patient taking medicine the stool specimen should be collected.
Sputum, Urine, Vaginal secretion, Duodenal fluid, Humores, CSF-----
Biopsy and animal inoculation
Summary
Principles of different methods of parasitic diagnosis. ( you should master them.)
Parasitic Pathogen Examination
寄生虫病原检查
Etioligic Diagnosis
Specimen:
Stool, blood, marrow, urine, sputum, humores(体液), secretion and tissue.
Purpose of examination
固定与染色
固定 薄血膜晾干后，甲醇固定
染色 将姬氏染液滴于已固定的薄血
膜上，染色半小时（室温），自 来水冲洗。血片晾干后镜检。
Blood and marrow examination
Examining microfilaria from blood
Time for blood collecting Methods:
Examining pathogen, identifying the species of parasite and the degree of infection.
Blood and marrow examination
Examining plasmodium
the collection of blood time：