SrRNA扩增常用引物

Normally, we used following primers to amplify bacterial 16S rRNA genes (27F and 1492R pair) and sequencing them (using other primers).

The primer sequencs are all listed in the reference:? Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115-175

27F 5' AGA GTT TGA TCM TGG CTC AG 3' PCR and sequencing, most eubacteria

357F 5' CTC CTA CGG GAG GCA GCA G 3' Most eubacteria

530F 5' GTG CCA GCM GCC GCG G 3' Most eubacteria and archaebacteria

926F 5' AAA CTY AAA KGA ATT GAC GG 3' Most eubacteria and archaebacteria

1114F 5' GCA ACG AGC GCA ACC C 3' Most eubacteria

342R 5' CTG CTG CSY CCC GTA G 3' Most eubacteria

519R 5' GWA TTA CCG CGG CKG CTG 3' Most eubacteria and archaebacteria

907R 5' CCG TCA ATT CMT TTR AGT TT 3' Most eubacteria and archaebacteria

1100R 5' GGG TTG CGC TCG TTG 3' Most eubacteria

1492R 5' TAC GGY TAC CTT GTT ACG ACT T 3' PCR and sequencing, most eubacteria

1525R 5' AAG GAG GTG WTC CAR CC 3' PCR and sequencing, most eubacteria

M=C:A, Y=C:T. K=G:T, R=A:G, S=G:C. W=A:T; all 1:1

Any primer's reverse complement sequence is its revers prime. For example:

519R 5' GWA TTA CCG CGG CKG CTG 3' then

519F 3' CWT AAT GGC GCC GKC GAC 5'