靶向磁性纳米粒子用于肿瘤的磁共振分子成像

中国医学科学院学报

ACT A ACADE M I A E MED I C I N AE SI N I CAE

124 Ap ril,2009

・论　著・

靶向磁性纳米粒子用于肿瘤的磁共振分子成像

陆菁菁1,王　芳1,金征宇1,钟定荣2

中国医学科学院　北京协和医学院　北京协和医院　1放射科　2病理科,北京100730

通信作者:金征宇　电话:010*********,电子邮件:jin_zhengyu@1631com

摘要:目的　探讨靶向磁性纳米粒子用于肿瘤磁共振分子成像的可行性。方法　将靶向部分重组人促性腺激素释放激素类似物与成像部分超顺磁性氧化铁纳米颗粒连接形成靶向探针,无靶向物质的成像部分作为对照材料。利用体外细胞实验和荷瘤裸鼠体内实验探索探针的靶向结合作用。体外细胞实验采用靶向探针和敏感肿瘤细胞株A549共同孵育,非靶向材料和同种细胞孵育作为对照,细胞洗脱后裂解,磁共振成像测量细胞裂解液的T2值。体内实验将靶向探针和非靶向材料分别注入实验组和对照组荷瘤裸鼠体内,磁共振成像动态检测肿瘤局部组织的信号和T2值变化。结果　体外细胞实验和肿瘤体内实验均证实靶向探针与非靶向颗粒溶液相比,与体外细胞和体内肿瘤组织有更强的结合能力。结论　肿瘤磁共振分子成像可使用靶向磁性纳米粒子作为探针,实现肿瘤的靶向成像,具有良好的发展前景。

关键词:磁性纳米颗粒;分子影像学;肿瘤;磁共振成像

中图分类号:R44512 文献标识码:A 文章编号:10002503X (2009)022*******

DO I:1013881/j 1issn 110002503X 120091021002

Targeted M agnetic Nanoparticles Used a s Probe for M agnetic

Resonance M olecular Imag i n g of Tum or

LU J ing 2jing 1,WANG Fang 1,J IN Zheng 2yu 1,ZHONG D ing 2rong

21Depart m ent of Radiology,2Depart m ent of Pathol ogy,PUMC Hosp ital,CAMS and PUMC,Beijing 100730,China

Corres ponding author:J I N Zheng 2yu Tel:010*********,E 2mail:jin_zhengyu@1631com

ABSTRACT:O bjective To investigate the feasibility of in vivo tumor detection using magnetic reso 2nance (MR )molecular i maging with targeted magnetic nanoparticles as i m aging p robe 1M ethods Targeted p robe was synthesized by covalently linking the recombinant human gonadotrop in releasing hor mone analog (the targeting portion )with the ultras mall superparamagnetic iron oxide nanoparticles (the i maging portion )1The i maging portion served as the control material 1The in vitro tumor cell experi ment and the in vivo experi m ent u 2sing nude m ice bearing tumors were carried out to test the targeting ability of the p robe 1In the in vitro experi 2ment,the targeting p robe and control materials were incubated separately with A549cells which had high affinity to gonadotrop in releasing hor mone 1Then the cells were taken out and lysed 1The resultant s olution was then sub 2jected to MR i maging 1The T2value of the s olutions was measured and compared 1In the in vivo experi ment,the targeting p robe was adm inistered into nude m ice bearing A549tumors 1Dynam ic MR i m aging was carried out to measure the signal and T2value of the tumor 1The control material was also adm inistered into control group of nude m ice,and dynam ic magnetic res onance i maging was perfor med 1The T2value of the tumor in both group s were recorded and compared 1Results Both the in vitro and in vivo experi ments p roved the targeting ability of 基金项目:国家自然科学基金(30600578)和北京市科技新星计划(2007A102)Supported by the National N atural Sciences Foundation of China (30600578)and the B eijing Nova Program (2007A102)