平邑甜茶叶片光合速率及叶绿素荧光参数对氯化镉处理的响应

中国农业科学 2010,43(15):3176-3183

Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.15.015

平邑甜茶叶片光合速率及叶绿素荧光参数

对氯化镉处理的响应

王 利1，2，杨洪强1，3，范伟国3，张 召2

（1山东农业大学资源与环境学院农业资源利用博士后流动站，山东泰安 271018；2山东农业大学林学院农业生态与环境重点实验室，

山东泰安 271018；3山东农业大学园艺科学与工程学院/作物生物学国家重点实验室，山东泰安 271018）

摘要：【目的】研究氯化镉处理对平邑甜茶叶片光系统Ⅱ（PSⅡ）活性、光合速率影响及其相互关系，为进一步揭示镉伤害机理提供理论依据。【方法】平邑甜茶在含不同浓度氯化镉1/2 Hoagland营养液中培养30 d后，

测定其叶片光合速率（Pn）、气孔导度、胞间CO2浓度和荧光参数等，分析氯化镉处理后这些参数间的关系。【结果】

在氯化镉处理下，平邑甜茶叶片光合速率和气孔导度显著降低，胞间CO2浓度增加，300 μs时的叶绿素荧光强度

（Fk）提高，PSⅡ最大光化学效率（Fv/Fm，φPo）、用于电子传递的量子产额（φEo）、光化学性能指数（PI ABS）以及

有活性的反应中心的密度（RC/CS）明显下降，并且这些参数的变化幅度随着氯化镉浓度的增加而提高；通径分析

显示，300 μs时的相对可变荧光强度（V K）及其可变荧光Fv占（J相的荧光强度Fj-O相的荧光强度Fo）振幅的

比例（W K）对Pn的直接作用高于其它荧光参数。【结论】氯化镉使平邑甜茶叶片PSⅡ供体侧、受体侧和反应中心

受到显著伤害，从而降低了PSⅡ活性和光合速率；在氯化镉处理下，V K和W K对Pn的直接作用比较大。

关键词：平邑甜茶；氯化镉；光合速率；光系统Ⅱ；叶绿素荧光

Effect of CdCl2 Treatment on Photosynthetic

Rate and Chlorophyll Fluorescence Parameters in

Malus hupehensis Leaves

WANG Li 1,2, YANG Hong-qiang 1,3, FAN Wei-guo3, ZHANG Zhao2

(1Post-Doctoral Mobile Station of Agricultural Resource Utilization, College of Resources and Environment, Shandong Agricultural

University, Taian 271018, Shandong; 2Key Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong

Agricultural University, Taian 271018, Shandong; 3State Key Laboratory of Crop Biology/College of Horticultural Science and

Engineering, Shandong Agricultural University, Taian 271018, Shandong)

Abstract: 【Objective】For discovering the mechanism of Cd damage on leaves of Malus hupehensis Rehd., the activity of photosystemⅡ (PSⅡ), net photosynthetic rate (Pn) and their correlation in leaves treated with CdCl2 were studied. 【Method】 After

30 days of treatment by CdCl2 in 1/2 Hoagland solution, the Pn, stomatal conductance (Gs), intercellular CO2 concentration (Ci) and

chlorophyll fluorescence parameters in leaves of Malus hupehensis Rehd. were measured, and the relationship between these

parameters under CdCl2 treatment were analyzed. 【Result】Under the treatment of CdCl2, the Pn and Gs reduced, the Ci and the fluorescence intensity Fk at 300 μs increased, and the maximum photochemistry efficiency of PSⅡ(Fv/Fm, φPo), the quantum yield

for electron transport (φEo) , the performance index on absorption basis (PI ABS) and the density of active reaction center (RC/CS) all

decreased significantly. Furthermore, the range of variation of these parameters increased with the increasing of CdCl2 concentration.

The direct effect of the relatively variable fluorescence intensity V K and the ratio of variable fluorescence Fv on the amplitude Fj-Fo

(W K) at 300 μs for Pn were higher than that of others through the path analysis. 【Conclusion】 CdCl2 damaged the sides of acceptor

and donor and the reaction centers of PSⅡ of leaves of Malus hupehensis Rehd. The activity of PSⅡand Pn decreased, and the direct

收稿日期：2009-12-02；接受日期：2010-03-01

基金项目：山东农业大学博士后项目、国家自然科学基金项目（30671452）

作者简介：王利，副教授，博士。E-mail：liwang6868@。通信作者杨洪强，教授。E-mail：hqyang@