抗核抗体与补体检测在系统性

抗核抗体与补体检测在系统性

红斑狼疮诊断中的意义

李冰,冯斐斐1,杨新周,聂广金1,张鹏2

郑州大学第一附属医院呼吸科　郑州市　450052

摘要　目的　探讨抗核抗体(ANA)和补体C3、C4检测在系统性红斑狼疮(S LE)诊断中的意义。方法　对我院52例S LE患者和40例健康体检者用间接免疫荧光法检测ANA,用散射比浊法检测C3、C4。结果　52例S LE患者的ANA阳性率为9213%,与对照组比较,差异有统计学意义(P<0105)。补体C3、C4值(珋x±s)分别为(0174±0134)、(0118±0109),比对照组低,差异有统计学意义(P<0105)。3个指标间相关关系为:C3与C4呈正相关关系,C4与ANA呈负相关关系,且相关有统计学意义(P<0105);而C3与ANA未呈现相关关系。判别分析得到的分类方程式为病例组:Y1=-431858+71316×性别+01425×年龄+141701×C3+571190×C4+ 101125×ANA;对照组:Y2=-361500+91520×性别+01397×年龄+191694×C3+441672×C4+41893×ANA;利用上述分类函数方程式,对病例和对照进行判断,判断S LE患者的符合率为8815%,判断正常对照的符合率为100%。结论　检测ANA、C3、C4对S LE有较高的诊断价值,其中C4的意义最大,在诊断S LE的实验室检测中是必不可少的3个指标。

关键词　系统性红斑狼疮;ANA;C3;C4;判别分析

中图分类号:R593124 文献标识码:B 文章编号:167223422(2008)0920006203

Si gn i fi cance of Anti-nucleosome Antibody and Co mplement

i n D i a gnosis of System i c Lupus Erythematosus

L I B ing,FEN G Feifei,YAN G X inzhou,et a l

The F irst A ffiliated Hospital,Zhengzhou U niversity,Zhengzhou450052,China

ABSTRACT　O bjecti ve　To exp l ore the r ole of anti-nucles ome antibodies(ANA)and com2 p le ment components C3、C4in the diagnosis of syste m ic lupus erythemat osus.M ethods　ANA was de2 tected by indirect i m munity fluorescent method and C3、C4were detected by nephel ometry in the seru m of52S LE and40health contr ols.Results　Out of52S LE,9213%were positive for ANA,there were significantly statistical differences bet w een the case gr oup and the contr ol gr oup(P<0105).The lev2 els of comp le ment C3、C4were(0174±0134)、(0118±0109)res pectively,which were l ower than those of contr ol gr oup,and there were statistical differences bet w een the t w o gr oup s(P<0105). There was the positive bivariate correlati on bet w een the seru m levels of C3and C4,negative bivariate correlati on bet w een the levels of C4and ANA in the S LE patients(P<0105).And there was not cor2 relati on bet w een C3and ANA.T wo equati ons were given by discri m inati on analysis,that were:case gr oup Y1=-431858+71316×sex+01425×age+141701×C3+571190×C4+101125×ANA; contr ol gr oup Y2=-361500+91520×sex+01397×age+191694×C3+441672×C4+41893×ANA;t o distinguish the t w o gr oup s by the t w o equati ons,the coincidence percentage was8815%in case gr oup and100%in contr ol gr oup.Conclusi on　ANA、C3and C4were very valuable in the diag2 nosis of S LE,es pecially C4.they were indis pensable markers in the diagnosis of S LE.

KE Y　WO R D S　Syste m ic lupus erythe mat osus;Anti-nucleos ome antibody(ANA);Co mp le2 ment C3、C4;D iscri m inati on analysis

1　郑州大学公共卫生学院劳动卫生教研室　郑州市　450001 2　郑州大学第一附属医院骨科　郑州市　450052

系统性红斑狼疮(S LE)是一种常见的累及多器官的自身免疫性结缔组织疾病。目前尽管S LE

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・　医药论坛杂志　2008年5月　第29卷　第9期