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Roche MagNA Lyser 产品说明书

Ordering Information Cat. No. Product ***********MagNA Lyser Instrument (230 Volt)***********MagNA Lyser Instrument (110 Volt)(Instruments supplied with rotor and rotor cooling block)***********MagNA Lyser Green Beads (100 tubes)Related Products Cat. No. Product***********MagNA Pure LC DNA Isolation Kit II (Tissue)***********MagNA Pure LC mRNA Isolation Kit II (Tissue)03 330 591 001MagNA Pure LC RNA Isolation Kit III (Tissue)***********MagNA Pure LC DNA Isolation Kit III (Bacteria, Fungi)***********MagNA Pure LC RNA Isolation Tissue Lysis Buffer – Refill (70 ml)System DescriptionHomogenize up to 16 samples in just a few seconds.Save valuable lab space with a small benchtop instrument.Reduce hands-on time by replacing the mortar and pestle and other manual methods.Integrate your workflow with the automated nucleic acid isolation of the MagNA Pure LC Instrument.Perform consistent and reproducible sample disruption.Process many different sample types.Prevent nucleic acid degradation with the benchtop cooling unit.Ease your setup with a removable rotor and prefilled disposable vials.Automate with an easy-to-use instrumentVersatile, efficient, and rapid pre-preparationFigure 71. Add your sample and lysis buffer to the MagNA Lyser Green Beads.2. Homogenize with the MagNA Lyser Instrument.3. Centrifuge to pellet the debris.4. Proceeed with the supernatant to prepare nucleic acids or proteins.For detailed information,visit or contact your local representative.Trademarks:MagNA Pure, MagNA Lyser, LightCycler, and the MagNA Pure Logo are trademarks of a member of the Roche Group.The technology used for the LightCycler System is licensed from Idaho Technology Inc., Salt Lake City, UT, USA.Fully automated sample preparationon the PCR Workflow SystemRoche Diagnostics GmbH Roche Applied Science Nonnenwald 282372 Penzberg Germany0000Roche Applied Science Part of Roche DiagnosticsMagNA Lyser InstrumentStart the Ball Rollingwith Automated Tissue HomogenizationᕤᕣᕢᕡFigure 6Components of the system.The MagNA Lyser InstrumentAutomated tissue homogenizationProcessing conditionsRefer to the following tables for guidelines on setting up your homogenizationSample material(10 mg)*Time settings(seconds)Cooling(between the runs)Speed Average yield(µg)***Average purity(OD 280/260 nm)***Spleen 2 x 25 906,00030–40 1.9Liver 25-6,00016–18 1.8Lung 2 x 25906,00025 1.8Kidney25-6,000201.8Maize leaves **20-5,00010n.d.Maize polenta **20-5,0008n.d.Tortilla chips **20-5,0001n.d.*Aliqout containing 10 mg sample material (here mouse and food samples) was taken for the DNA purificationusing the MagNA Pure LC DNA Isolation Kit II (Tissue), (see pack insert)**Centrifugation after the homogenization for 5 minutes at 2,200 x g*** Yield and purity strongly depend on the condition of the sample material n.d.not determinedData kindly provided by Dr. Peterhänsel, RWTH Aachen, GermanyFigure 1Gel electrophoresis from genomic DNA isolated from tissue homogenized with the MagNA LyserInstrument, using the MagNA Pure LC DNA Kit II (Tissue).Marker: DNA Marker III*Aliquot containing 10 mg sample material (here mouse and human research samples) was taken to purify RNAeither with the MagNA Pure LC RNA Isolation Kit III (Tissue) or the MagNA Pure LC mRNA Isolation Kit II (Tissue) homogenized with the MagNA Lyser Instrument.** Yield and purity strongly depend on the condition of the sample material. The yield for mRNA was not determined.Sample material(10 mg)*Time settings(cycles/seconds)Cooling(between/afterthe runs in seconds)SpeedAverage yield (mg)(total RNA)**Average purity(OD 280/260 nm)**RNA/mRNARarely expressed targets in small numbers of target cells,as seen in experiments about minimalresidual diseases,are difficult to detect.Increasing the cell number can improve sensitivity and lead to accurate results.Without the MagNA Lyser pre-processing,the MagNA Pure mRNA HS Kit can efficiently obtain mRNA from a maximum of 1 x 107white blood cells (WBCs),as shown in research studies with human samples.However,using greater cell numbers results in a saturation effect with quantitative assays (Figure 3).Homogenization of the lysate with the MagNA Lyser Instrument prior to the purification eliminatesthe amplification saturation at 1 x 107cells and allows the use of up to 2.5 x 107WBCs (Figure 4 and 5),enhancing the analytical sensitivity of the assay.Eliminate sensitivity barriers with increased sample inputFigure 3mRNA was purified from different amounts of human white blood cells with the MagNA Pure mRNA HS Kit. G6PDH was amplified using the LightCycler t(9;22) Quantification Kit (see text beside).Figure 4mRNA was purified from different amounts of human white blood cells with the MagNA Pure mRNA HS Kit. The lysates from 2.5 x 107cells and 5 x 107cells were homogenized with the MagNA Lyser Instrument (2x50 seconds with 90 seconds cooling in between) prior to the mRNA purification. G6PDH was amplified using the LightCycler t(9;22) Quantification Kit (see text beside).Figure 5Scalability from 1 x 106cells to 2.5 x 107cells is represented in the graph and the table of the relationship between crossing points and cell numbers. The limitation of cell input is indicated by no change in crossing point with increased cell number (see text beside).Cell number 5 x 1072.5 x 1071 x 1075 x 1061 x 106Log (cell number)7.77.47.06.76.0Crossing point 20.320.321.822.424.4crossingpointLog(cell number)252423222120195.86.36.87.37.8Figure 2Gel electrophoresis from total RNA isolated from tissue homogenized with the MagNA Lyser Instrument, using the MagNA Pure LC RNA Kit III (Tissue).Ma r k e rS p l e e nL i v e rL u n gK i d n e yM a r k e rMa i z e l e a v e sMa i z e l e a v e sS p l e e nL i v e r11 kb5 kb5 kb28 S rRNA 18 S rRNASpleen 2 x 50 90 6,500–7,000 30–40 1.9Liver 50 - 6,500–7,000 13–17 2.0Thymoid tissue60906,500n.d.n.d.Heart 60 90 6,500 n.d. n.d.Abdominal fat 60 90 6,500 n.d. n.d.Aorta 60 90 6,500 n.d. n.d. Other samples1+n x 50 90 6,500–7,000- -1 x 105 x 101 x 10- 5 x 101 x 105 x 105 x 10- 5 x 102.5 x 10 5 x 10。

hk3ffdc12vshg工作原理

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文档下载后可定制随意修改，请根据实际需要进行相应的调整和使用，谢谢!并且，本店铺为大家提供各种各样类型的实用资料，如教育随笔、日记赏析、句子摘抄、古诗大全、经典美文、话题作文、工作总结、词语解析、文案摘录、其他资料等等，如想了解不同资料格式和写法，敬请关注!Download tips: This document is carefully compiled by the editor. I hope that after you download them, they can help you solve practical problems. The document can be customized and modified after downloading, please adjust and use it according to actual needs, thank you!In addition, our shop provides you with various types of practical materials, such as educational essays, diary appreciation, sentence excerpts, ancient poems, classic articles, topic composition, work summary, word parsing, copy excerpts, other materials and so on, want to know different data formats and writing methods, please pay attention!HK3ffdc12vshg是一种先进的工作原理，它在各个领域都有着广泛的应用。

HK基因综述课件

PCR几小时便可完成。PCR技术是生物医学领
域中的一项革命性创举和里程碑。
2024/5/4
《HK基因综述》PPT课件
2
RT—PCR（ Reversed Transcript PCR ）是一种将cDNA合成与PCR技术结合分析基因表达
的快速灵敏的方法，主要用于对表达信息进行
检测或定量分析,还可以 and Validation of Candidate Housekeeping Genes for Studies of Human Keratinocytes—Review and Recommendations
Michael B鋜1, Dorit B鋜2 and Bodo Lehmann1（2009）
Validation of housekeeping genes for normalizing RNA expression in real-time PCR。
Keertan Dheda1, Jim F. Huggett1, Stephen A. Bustin2,
Margaret A. Johnson1, Graham Rook1, and Alimuddin Zumla1 （2004）
组样本能够证明在哺乳动物物种中新的候选
HKG增加了稳定性。
Evidence Based Selection of Housekeeping Genes
HM20e2.4nW/5d/.4rHikoJf.sMtra. 2d, eFJroannsgeG1e.,rRbeundso2l,f WS《.ilNHleK. m基Fe因Ah综r. m述Ka》anmPnPpT2s课,31件,,4E.,lEisvaebleinthe
为了了解HK基因目前的研究状况和HK基因研究的主要方法，下面介绍目前国内外对于HK基因的研究情况。

Eurofins MTS Consumer Product Testing Hong Kong Li

Contact DetailsRegistration No. HOKLAS 129Page 1 of 1 129TESTEurofins MTS Consumer Product Testing Hong Kong Limited歐陸現代商品檢測(香港)有限公司ADDRESS 地址: 6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室ENQUIRY 查詢:Mr CHEUNG Chi-kwan, Quality Assurance Manager 張智君先生，品質經理TELEPHONE電話: 3604 1363FAX傳真: 2742 3735E-MAIL電郵: *******************WEBSITE ADDRESS網址: CLIENTELE 服務對象: Public 公眾Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited歐陸現代商品檢測(香港)有限公司 129TESTADDRESS 地址: 6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室ACCREDITED TEST CATEGORY認可測試類別: Chemical Testing 化學測試Textiles and Garments 紡織品及成衣Toys and Children’s P roducts 玩具及兒童產品Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Chemical Testing 化學測試ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests: -Plastics Cadmium content Regulation (EC) No. 1907/2006 on REACH Annex XVII Item No 23 as amended by Commission Regulation (EU) No. 494/2011Compliance test procedures for cadmium content: EN 1122: 2001 Method B (wet decomposition) BS EN 1122: 2001 Method B (wet decomposition)Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Chemical Testing 化學測試ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests: - (cont ’d)Products made from leather products Amine (Azo colorants) : Regulation (EC) No. 1907/2006 on REACH AnnexXVII Item No 43 as amended by Commission 1) 4-aminodiphenyl Regulation (EC) No. 552/2009 & 126/2013 2) benzidine3) 4-chloro-o-toluidine Test procedures for compliance testing for 4) 2-naphthylamine leather products:5) o-aminoazotoluene ISO 17234-1: 2010 (for determination of the amines 6) 5-nitro-o-toluidine listed on the left and does not include 7) 4-chloroaniline4-aminoazobenzene)8) 4-methoxy-m-phenylenediamine ISO 17234-2: 2011 (for determination of 9) 4,4’-methylenedianiline 4-aminoazobenzene) 10) 3,3’-dichlorobenzidine11) 3,3’-dimethoxybenzidine Techniques used are GC-MSD and HPLC-DAD for all 12) 3,3’-dimethylbenzidinetest methods listed above 13) 4,4’-methylene-di-o-toluidine 14) p-cresidine15) 4,4’-methylene-bis-(2-chloro-aniline) 16) 4,4’-oxydianiline 17) 4,4’-thiodianiline 18) o-toluidine19) 4-methyl-m-phenylendiamine 20) 2,4,5-trimethylaniline 21) o-anisidine22) 4-aminoazobenzeneRef: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Chemical Testing 化學測試ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests: - (cont ’d)Textile products Amine (Azo colorants) : Regulation (EC) No. 1907/2006 on REACHAnnex XVII Item No 43 as amended by 1) 4-aminodiphenyl Commission Regulation (EC) No. 552/20092) benzidineand Commission Regulation (EC) No. 2020/2096 3) 4-chloro-o-toluidine 4) 2-naphthylamine5) o-aminoazotoluene Test procedures for compliance testing of 6) 5-nitro-o-toluidine textile p roducts:7) 4-chloroanilineEN ISO 14362-1: 2017 (for determination of the amines 8) 4-methoxy-m-phenylenediamine listed on the left except 4-aminoazobenzene) 9) 4,4’-methylenedianiline EN ISO 14362-3: 2017 (for determination of 10) 3,3’-dichlorobenzidine 4-aminoazobenzene) 11) 3,3’-dimethoxybenzidine12) 3,3’-dimethylbenzidineTechniques used are GC-MSD and HPLC-DAD for 13) 4,4’-methylene-di-o-toluidine all test methods listed above 14) p-cresidine15) 4,4’-methylene-bis-(2-chloro-aniline) 16) 4,4’-oxydianiline 17) 4,4’-thiodianiline 18) o-toluidine19) 4-methyl-m-phenylendiamine 20) 2,4,5-trimethylaniline 21) o-anisidine22) 4-aminoazobenzeneRef: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Chemical Testing 化學測試ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests: - (cont ’d)Products made of textile Amine (Azo colourants): GB 18401-2010 Cls. 5.1,附录C and 6.8 紡織品芳香胺(偶氮染料)1) 4-氨基聯苯(4-aminodiphenyl) Test procedures for compliance testing :2) 聯苯胺(benzidine)GB/T 17592-2011 (for determination of the amines 3) 4-氯鄰甲苯胺( 4-chloro-o-toluidine) listed on the left and does not include 4) 2-萘胺(2-naphthylamine)4-aminoazobenzene)5) 鄰氨基偶氮甲苯(o-aminoazotoluene) GB/T 23344-2009 (for determination of 6) 5-氨基-4-硝基甲苯(5-nitro-o-toluidine) 4-aminoazobenzene) 7) 對氯苯胺(4-chloroaniline)8) 2,4-二氨基苯甲醚Techniques used are GC-MSD and HPLC-DAD (4-methoxy-m-phenylenediamine) 9) 4,4’-二氨基二苯甲烷 (4,4’-methylenedianiline) 10) 3,3’-二氯聯苯胺(3,3’-dichlorobenzidine) 11) 3,3’-二甲氧基聯苯胺 (3,3’-dimethoxybenzidine) 12) 3,3’-二甲基聯苯胺 (3,3’-dimethylbenzidine) 13) 4,4’二氨基二苯甲烷(4,4’-methylene-di-o-toluidine) 14) 3-氨基對甲苯甲醚(p-克利酊) (p-cresidine)15) 4,4’-次甲基-雙-(2-氯苯胺)(4,4’-methylene-bis-(2-chloro-aniline)) 16) 4,4’-二氨基二苯醚(4,4’-oxydianiline) 17) 4,4’-二氨基二苯硫醚 (4,4’-thiodianiline)18) 鄰甲苯胺(o-toluidine) 19) 2,4’-二氨基甲苯(4-methyl-m-phenylendiamine) 20) 2,4,5-三甲基苯胺 (2,4,5- trimethylaniline)21) 鄰甲基苯胺(鄰氨基苯甲醚) (o-anisidine)22) 4-氨基偶氮苯(4-aminoazobenzene) 23) 2,4-二甲基苯胺(2,4-xylidine) 24) 2,6-二甲基苯胺(2,6-xylidine)Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Chemical Testing 化學測試ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests: - (cont ’d)Products made of leather and fur Amine (Azo colourants): GB20400-2006 Cl. 5, 附录A & Cl. 6.1 皮革和毛皮芳香胺(偶氮染料)1) 4-氨基聯苯 (4-aminodiphenyl) Test procedures for compliance testing : 2) 聯苯胺 (benzidine)GB/T 19942-2005 3) 4-氯鄰甲苯胺( 4-chloro-o-toluidine)4) 2-萘胺(2-naphthylamine)Techniques used are GC-MSD and HPLC-DAD 5) 鄰氨基偶氮甲苯(o-aminoazotoluene) 6) 5-氨基-4-硝基甲苯(5-nitro-o-toluidine) 7) 對氯苯胺(4-chloroaniline) 8) 2,4-二氨基苯甲醚(4-methoxy-m-phenylenediamine) 9) 4,4’-二氨基二苯甲烷 (4,4’-methylenedianiline) 10) 3,3’-二氯聯苯胺(3,3’-dichlorobenzidine) 11) 3,3’-二甲氧基聯苯胺 (3,3’-dimethoxybenzidine) 12) 3,3’-二甲基聯苯胺 (3,3’-dimethylbenzidine) 13) 4,4’二氨基二苯甲烷(4,4’-methylene-di-o-toluidine) 14) 3-氨基對甲苯甲醚(p-克利酊) (p-cresidine)15) 4,4’-次甲基-雙-(2-氯苯胺)(4,4’-methylene-bis-(2-chloro-aniline)) 16) 4,4’-二氨基二苯醚(4,4’-oxydianiline) 17) 4,4’-二氨基二苯硫醚 (4,4’-thiodianiline)18) 鄰甲苯胺(o-toluidine) 19) 2,4’-二氨基甲苯(4-methyl-m-phenylendiamine) 20) 2,4,5-三甲基苯胺 (2,4,5-trimethylaniline)21) 鄰甲基苯胺(鄰氨基苯甲醚) (o-anisidine)22) 2,4-二甲基苯胺(2,4-xylidine)23) 2,6-二甲基苯胺(2,6-xylidine)Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Textiles and Garments 紡織品及成衣ITEM TESTED OR MEASURED測試或量度項目SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Care performance test :-General textiles and garments - Dimensional stability to washing BS EN 5077: 2008 一般紡織品及成衣 ISO 5077-2007BS EN ISO 6330: 2021<Excluding the following>Cl. 5.1.2, 5.1.3, 5.2.2, 5.2.3 and 10.3- Wash & wear performance AATCC Test Method 124-1996AATCC Test Method 124-2006 AATCC Test Method 124-2011 AATCC Test Method 124-2014 AATCC Test Method 124-2018 AATCC Test Method 124-2018tChemical analysis:-General textiles and garments - Free formaldehyde content GB/T 18401-2010 Cl. 6.1一般紡織品及成衣游離甲醛含量 Test procedure for compliance testing: GB/T 2912.1-2009- pH value GB/T 18401-2010 Cl. 6.2pH 值 Test procedure for compliance testing: GB/T 7573-2009Other Materials- Leather and fur - Formaldehyde content GB 20400-2006 Cl. 5皮革和毛皮甲醛含量 Test procedure for compliance testing: GB/T 19941.2-2019<Excluding the following> Cl. 6.5Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Textiles and Garments 紡織品及成衣ITEM TESTED OR MEASURED測試或量度項目SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Colour fastness test :-General textiles and garments - Perspiration AATCC Test Method 15-2021一般紡織品及成衣耐汗渍色牢度 (rating determined by AATCC Gray Scale) AS 2001.4.E04-2005 (Reconfirmed) 2016-08-05 BS EN ISO 105-E04: 2013(rating determined by AATCC Gray Scale) GB/T 3922-2013- RubbingAATCC Test Method 8-2016耐摩擦色牢度 (rating determined by AATCC Gray Scale) AS 2001.4.3-1995 (Reconfirmed) 2016-08-05 BS EN ISO 105 X12: 2016 GB/T 3920-2008<Excluding the following>tests for carpets or textile floor coverings- SalivaGB/T 18886-2019 耐唾液色牢度- Washing AATCC Test Method 61-2013e (2020)(rating determined by AATCC Gray Scale)- WaterAATCC Test Method 107-2013耐水色牢度 (rating determined by AATCC Gray Scale) AS 2001.4.E01-2001 (Reconfirmed) 2016-08-05 BS EN ISO 105-E01: 2013(rating determined by AATCC Gray Scale) GB/T 5713: 2013Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Textiles and Garments 紡織品及成衣ITEM TESTED OR MEASURED測試或量度項目SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Construction test :-General textiles and garments - Mass per unit area AS 2001.2.13 - 1987 (Reconfirmed) 2016-08-05 一般紡織品及成衣布重 <Excluding the following> Method 8.2ASTM D3776/D3776M-20 Option C BS EN 12127: 1998<Excluding the following> Method 8.4GB/T 4669-2008方法5- Linear density of yarn removed from woven ASTM D1059-17fabric<Excluding the following> 織物中拆下紗線線密度 Sections 9.5 and 9.6BS ISO 7211-5: 2020 Method A FZ/T 01093-2008方法A- Threads per unit length of woven fabric AS 2001.2.5-1991 (Reconfirmed) 2016-08-05 機織物密度 Test AASTM D3775-17e1BS EN 1049-2: 1994 Method A GB/T 4668-1995方法A ISO 7211/2-1984 Method ARef: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Textiles and Garments 紡織品及成衣ITEM TESTED OR MEASURED測試或量度項目SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Flammability test :-General textiles and garments - 45° angle test ASTM D1230-22一般紡織品及成衣 US 16 CFR Part 1610- Vertical test US 16 CFR Part 1615 US 16 CFR Part 1616Performance test :-General textiles and garments - Abrasion resistanceAS 2001.2.25.2-2006 (Reconfirmed) 2016-08-05 一般紡織品及成衣 (Martindale Abrasion Tester Method) AS 2001.2.25.3-2006 (Reconfirmed) 2016-08-05 AS 2001.2.25.4-2006 (Reconfirmed) 2016-08-05 ASTM D4966-12 (2016) BS EN ISO 12947-2: 2016 BS EN ISO 12947-3: 1999 BS EN ISO 12947-4: 1999- Bursting strength (Hydraulic Method) AS 2001.2.4-1990 (Reconfirmed) 2016-08-05 <Excluding the following> Method BASTM D3786/D3786M-18 BS EN ISO 13938-1: 2019- Odour GB 18401-2010 Cl. 6.7 異味- Pilling resistanceASTM D3512/D3512M-16 (Random tumble pilling tester)- Propensity to surface fuzzing and to pilling BS EN ISO 12945-1: 2020 織物起毛起球性能 GB/T 4802.3-2008Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Textiles and Garments 紡織品及成衣ITEM TESTED OR MEASURED測試或量度項目SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Strength test :-General textiles and garments - Tensile strength (Grab Method) AS 2001.2.3.2-2001 (Reconfirmed) 2016-08-05 一般紡織品及成衣織物拉伸性能(抓樣法) ASTM D5034-21BS EN ISO 13934-2: 2014 GB/T 3923.2-2013- Tearing strength (Elmendorf Method) AS 2001.2.8-2001 (Reconfirmed) 2016-08-05 織物撕破性能(冲擊擺錘法) ASTM D1424-21BS EN ISO 13937-1: 2000 GB/T 3917.1-2009Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests :-Toys made of plastics Cadmium content Regulation (EC) No. 1907/2006 on REACH Annex XVII Item No. 23 as amended by Commission Regulation (EU) No. 494/2011Test procedures for compliance testing :EN 1122: 2001 Method B (wet decomposition) BS EN 1122: 2001 Method B (wet decomposition)All toys - Migration of the elementsISO 8124-3: 2020 (for dewaxing: Method A only)Antimony, Arsenic, Barium, Cadmium, AS/NZS ISO 8124.3: 2021 (for dewaxing: Method A only ) Chromium, Lead, Mercury, SeleniumAll toys - paints and similar surface - Total Lead content ASTM F963-17 Cl 4.3.5.1(1)coating materials Test procedures for compliance testing:CPSC Test method: CPSC-CH-E1003-09.1 Standard Operating Procedure for Determining Lead (Pb) in Paint and Other Similar Surface Coatings, February 25, 2011- Migration of the elementsASTM F963-17 Cl. 4.3.5.1 (2)Antimony, Arsenic, Barium, Cadmium, Test procedures for compliance testing: Chromium, Lead, Mercury, Selenium Cl. 8.3.2-8.3.4Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests: - (cont ’d)Toys – substrate materials - Total lead content ASTM F963-17 Cl 4.3.5.2.(2)(a)Test Procedures for Compliance testing: CPSC Test Method: CPSC-CH-E1001-08.3Standard Operating Procedure for Determining TotalLead (Pb) in Children’s Metal Products (Including Children’s Metal Jewelry), November 15, 2012 <Excluding the following> XRF SpectrometryCPSC Test Method: CPSC-CH-E1002-08.3Standard Operating Procedure for Determining Total Lead (Pb) in Non-Metal Children ’s Products , November 15, 2012<Excluding the following> XRF Spectrometry- Migration of the elementsASTM F963-17 Cl 4.3.5.2 (2)(b)Antimony, Arsenic, Barium, Cadmium, Test procedures for compliance testing:- Chromium, Lead, Mercury, Selenium Clause 8.3.5Metallic toys or metallic toy components - Extractable Cadmium ASTM F963-17 Cl. 4.3.5.2 (2) (c)Test Procedures for Compliance testing: Cl. 8.3.5.5(3) andCPSC Test Method: CPSC-CH-E1004-11Standard Operating Procedure for DeterminingCadmium (Cd) Extractability from Children’s Metal Jewelry, February 03, 2011 (with modification using Flame AAS for determination)Toys and other articles intended for use by - Total lead content 16 US CFR 1303children – paint and other similar US Public Law 110 -314surface-coating materials Consumer Product Safety Improvement Act 2008 Test procedures for compliance testing:CPSC Test method: CPSC-CH-E1003-09.1 Standard Operating Procedure for Determining Lead (Pb) in Paint and Other Similar Surface Coatings, February 25, 2011Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests: - (cont ’d)Children’s metal products- Total lead contentUS Public Law 110 -314Consumer Product Safety Improvement Act 2008Test Procedures for Compliance testing : CPSC Test Method: CPSC-CH-E1001-08.3Standard Operating Procedure for Determining Total Lead (Pb) in Children’s Metal Products(Including Children’s Metal Jewelry),November 15, 2012<Excluding the following> XRF SpectrometryChildren’s non -metal products - Total lead contentUS Public Law 110 -314Consumer Product Safety Improvement Act 2008 Test Procedures for Compliance testing : CPSC Test Method: CPSC-CH-E1002-08.3Standard Operating Procedure for Determining Total Lead (Pb) in Non-Metal Children’s Produc ts, November 15, 2012<Excluding the following>XRF SpectrometryToys and childcare articles Di-(2-ethylhexyl) phthalate (DEHP) US Public Law 110 -314Dibutyl phthalate (DBP)Consumer Product Safety Improvement Act 2008 and Benzyl butyl phthalate (BBP) US 16 CFR Part 1307, Title I, Section 108 Diisononyl phthalate (DINP) Test Procedures for Compliance testing : Di-n-pentyl phthalate (DPENP) CPSC Test Method: CPSC-CH-C1001-09.4Di-n-hexyl phthalate (DHEXP) Standard Operation Procedure for Determination Dicyclohexyl phthalate (DCHP) of Phthalate, January 17, 2018 Diisobutyl phthalate (DIBP)Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests :- (cont ’d)All toys and children ’s products - - Total Lead content Canada Consumer Product Act – Toys Regulations, surface coating materials Item 23, SOR/2011-17 February 3, 2011 Test procedures for compliance testing:Health Canada Product Safety Reference manual Book 5–Laboratory Policies and Procedures Part B: Test Method Section, Method C-02.2:Determination of Total Lead in Surface Coating Materials in Consumer Products (2008-11-3)- Total Mercury content Test procedures for compliance testing: In-house method: SOP-0058- Leachable Arsenic, Selenium, Cadmium, Test procedures for compliance testing:Antimony and Barium Health Canada Product Safety Reference manual Book 5 – Laboratory Policies and Procedures Part B: Test Methods Section, Method C-03:Determination of Leachable Arsenic (As), Selenium (Se), Cadmioum (Cd), Antimony (Sb) and Barium (Ba) in Applied Coatings (2009-06-15)Surface coating - Total Lead content The Surface Coating Materials Regulations, SOR/2005-109, with Amds. SOR/2011-14,February 4, 2011, Articles with a surface coating material, 7.2 & 7.3Test procedures for compliance testing:Health Canada Product Safety Reference manual Book 5–Laboratory Policies and Procedures Part B: Test Method Section, Method C-02.2:Determination of Total Lead in Surface Coating Materials in Consumer Products (2008-11-3)Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Chemical tests :- (cont ’d)Toys- Migration of certain elements BS EN 71-3:2019+A1:2021 Category III toy materials: EN 71-3:2019+A1:2021 Scraped-off materials- Aluminium, Antimony, Arsenic, Barium, Technique used: ICP-MS Boron, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury,Nickel, Selenium, Strontium, Tin and Zinc- Chromium (for screening for Cr(VI)) Alternative method as documented in SOP-0001d (ICP-MS)- Organic Tin:Methyl tin, Dimethyl tin, Butyltin, Dibutyl tin, Technique used: GC-MS Tributyl tin, Tetrabutyl tin, n-Octyl tin, Di-n-octyl tin, Di-n-propyl tin, Diphenyl tin, Triphenyl tinRef: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Electrical tests :-Battery operated toy - Battery operated toys safety tests ASTM F963-17 Cl. 4.25 <Excluding the following> Cl. 4.25.8.1, 4.25.10, 4.25.11Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Flammability tests :-Toys AS/NZS ISO 8124.2: 2016 ISO 8124-2: 2014<Excluding the following> for the above two standardsTests for flammable gel, extremely flammable liquid, highly flammable liquid, flammable liquid and flammable gasBS EN 71-2: 2020 EN 71-2: 2020<Excluding the following> for the above two standardsTests for flammable gel, extremely flammable liquid, highly flammable liquid, flammable liquid and flammable gasASTM F963-17 Cl. 4.216 CFR Part 1500.44Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Physical and mechanical tests :-Toys - Safety requirements and test methods AS/NZS ISO 8124.1:2019 + A1:2020 + A2:2020<Excluding the following>Cl. 4.8.2, 4.16.2.2, 4.18.4.1, 4.21, 4.23, 4.26c, 4.28, 4.29f and 4.29g ISO 8124.1: 2018 + A1:2020 + A2:2020<Excluding the following>Cl. 4.8.2, 4.16.2.2, 4.18.4.1, 4.21, 4.23, 4.26c, 4.28, 4.29f and 4.29gBS EN 71-1: 2014 + A1: 2018 EN 71-1: 2014 + A1: 2018<Excluding the following>Cl. 4.11e) 4.14.1c), 4.15.1.5, 4.15.1.8, 4.18 (test to EN 15649-3), 4.19, 4.20.2.5 and 4.20.2.12- Safety Requirements: ASTM F963-17 Section 4.1, 4.3.7, 4.5, 4.6, 4.7, 4.8, 4.9, 4.10, 4.11, 4.12, 4.13, 4.14, 4.15, 4.16, 4.17, 4.18, 4.19, 4.20, 4.21, 4.22, 4.23, 4.24, 4.26, 4.27, 4.28, 4.30, 4.31, 4.32, 4.33, 4.34, 4.35, 4.36, 4.38, 4.39 and 4.40<Excluding the following>Section 4.1 (examination of reprocessed materials to4.3.1), 4.5.1.6, 4.6.2.2, 4.8.1 and 4.20.1 - Safety labeling requirements Section 5 - Instructional literature Section 6 - Producer’s markings Section 7 - Test Methods Section 8Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Physical and mechanical tests :- (cont’d)Toys (cont’d) - Technical requirements for determining a 16 CFR 1500.48 sharp point in toys intended for use by children under 8 years of age- Technical requirements for determining a 16 CFR 1500.49 sharp metal or glass edge in toys intended for use by children under 8 years of age- Test methods for simulating use and abuse 16 CFR 1500.50, 1500.51, 1500.52 and 1500.53 of toys intended for use by children - Impact test - Bite test - Flexure test - Torque test - Tension test- Compression testToys and other articles intended for use - Method for identifying choking, aspiration, 16 CFR 1501 in conjunction withby children under 3 years of age or ingestion hazards because of small parts test methods specified in 16 CFR 1500.51 or 16 CFR 1500.52 [excluding bite test to 1500.51(c) or 1500.52 (c)]Infant rattles - Method for identifying choking and/or 16 CFR 1510 in conjunction withsuffocation hazards test methods specified in 16 CFR 1500.51 [excluding bite test to 1500.51(c) and flexure test to 1500.51 (d)]Pacifiers - Requirement for pacifiers 16 CFR 1511Ref: HOKLAS129-68Eurofins MTS Consumer Product Testing Hong Kong Limited129TEST6/F, 7/F & Unit 801-816 of 8/F, CEO Tower, 77 Wing Hong Street, Cheung Sha Wan, Kowloon, Hong Kong 香港九龍長沙灣永康街77號環薈中心6樓、7樓及8樓801-816室Toys and Children ’s Products 玩具及兒童產品ITEM TESTED OR MEASURED測試或量度項目 SPECIFIC TEST OR PROPERTY MEASURED 特定測試或量度的特性SPECIFICATION, STANDARD METHODOR TECHNIQUE USED 規範、標準方法或應用技術Physical and mechanical tests :- (cont’d)Toys (cont’d) - Safety Requirements: Canada Toys Regulations (SOR/2011-17) (last amended on11 January 2019) with relevant amendments specified in Regulation SOR/2018-138Section 3, 4, 7, 8 , 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 28, 29, 30, 31, 37, 40, 41, 42, 43 and 44 <Excluding the following> Section 29(c), 29(d)Health Canada Product Safety Reference Manual Book 5 – Laboratory Policies and Procedures Part B: Test Method Section- Small components Method M00.1 (2019-07-02) - Sharp edges Method M00.2 (2021-04-22) - Sharp pointsMethod M00.3 (2021-01-15) - Eye/nose security - dolls, plush toys and soft Method M00.4 (2020-05-06) toys- Reasonably foreseeable useMethod M01.1 (2019-02-18) - Test method for flexible film bagsMethod M03 (2016-12-20) - Test method to determine the noise level of toys Method M04 (2017-02-21) - Test method for rattles Method M05 (2021-04-27) - Magnetic flux index Method M18 (2021-02-04)(Note: The above test methods should be used in conjunction with the relevant sections of Canada Toys Regulations (SOR/2011-17))- Kite string Canada Consumer Product Safety Act S.C. 2010, c.21 Schedule 2, Item 9 (Test to EN 71-1:2011 Cl. 4.13)。

常见HTML标记及含义

一、html基础知识二、html结构<html><head><title>网络0801</title></head><body></body></html>基本标记.<p></p> 段落<br> 换行三、head内的标记1、title 标题标记功能：标题控制使用：设置标题格式：<title>标题内容</title>实例：添加一个标题2、base 基底网址标记功能：文档的基本网址，其他为与其相对的网址使用：插入栏/html/文件头/基础格式：<base href="" target="父(_P)" />实例：设置后，在文章中添加一个相对路径和绝对路径4、media 原信息标记功能：提高被搜索引擎搜索到的可能性使用：插入栏/html/文件头/meta【name-搜索引擎http-equiv-设置浏览器检查信息，控制网页精确显示】格式：<meta name="author" content="宋赟中" />（作者）<meta name="keywords" content="网络，计算机学校" />（关键字）<meta name="description" content="忙忙忙忙忙忙忙忙忙忙" />（说明）<meta name="build" content="建设时间"/><meta name="reply-to" content="联系人邮箱"/><meta name="copyright" content="版权说明"/><meta http-equiv="Content-Type" content="text/html; charset=gb2312" />（字符集）<meta name=<meta http-equiv="refresh" content="22" />（刷新）<meta http-equiv="windows-target" content="_top" />（强制独立窗口打开）<meta http-equiv="pragma" content="No-cach />（页面不允许缓存）实例：设置后，会提高搜索引擎的命中率四、body标记1、bgcolor 背景色2、background 背景图像3、text 文字颜色4、bgproperties 背景滚动方式fixed 代表固定5、link 默认链接颜色6、alink 鼠标点击时的颜色7、vlink 点击后的颜色8、topmargin 页面上边距9、leftmargin 页面左边距五、注释语句1、 <comment></ comment >2、3、<plaintext> 将原代码显示在浏览器中4、<ruby> ssssssssssss<rt>ddddd</rt></ruby> rt的注释内容出现在ruby上方5、、/* */ css注释六、文本标记1、<h数字></h数字> 标题字标记包含对齐（align）属性2、<strong> </strong> 加粗标记3、<em></em>倾斜4、<s></s> 删除线5、<u></u> 下划线6、< sup ></ sup > 上标7、< sub ></ sub > 下标8、< big ></ big > 大字号7、< small ></ small > 小字号8、<font face="字体" size="字号" color="颜色"> </font> 文字标记9、<p></p> 段落标记具备align属性10、<pre> </pre> 预格式化（使得代码与页面的显示一致）11、<center></center> 居中12、<hr align="对齐" width="宽" size="高" noshade="阴影" color/> 水平线13、<kbd> </kbd> 在浏览器中（如标签后）显示的信息14、<address> </address>在文档中显示作者的信息15、<tt> </tt> 打字字体16、<ul> 项目列表<li type="circle">fdfsdfs</li> 空心圆<li type="disc">fsfsfs </li> 圆<li type="square">kjkjhkhj</li> 方</ul>17、<ol type="1" start="2"> 编号列表，type代表显示什么，start代表从那开始<li>fdfsdfs</li><li>fsfsfs </li></ol></body>18、<dl> 定义列表（产生类似文字说明的效果）<dt>fdfsdfs</dt> 内容<dd>fsfsfs </dd> 说明<dt>kjkjhkhj</dt><dd>hfhfhfhfh</dd></dl>19、<ol> 列表嵌套（选中内容，缩进）<li>dadasd</li><li>adsasdsa<ol><li>dasda</li><li>sdaad</li></ol></li></ol>七、图片标记1、<img src="banner资料.jpg" alt="aaa" width="100" height="200" hspace="2" vspace="1" border="2" align="middle" lowsrc="../images/cpfl_04.gif" longdesc="" />Src 图片文件的路径alt 说明文字width 宽度height 高度hspace 水平边距vspace 垂直边距border 边框宽度align 文字环绕lowsrc 低解析度源文件2、<br clear="left" />代码加到对应前边可以取消环绕3、<img src="../images/未标题-1_03.gif" usemap="#Map" /> 应用区域<map name="Map" id="Map"> 使用热点<area shape="circle" coords="81,54,16" href="#" /> 定义热点区</map>Cords 区域边界href 链接shape（热点类型）circle圆rect 矩形poly 多边形主要用于电子地图和导航条八、链接1、<a href="" target="_parent" name="链接名" title="链接提示文字">fhgfhf</a> href 链接target 打开方式name 连接名title 链接提示文本链接（）2、<a name="mm" id="mm"></a> 锚点链接3、<a href="maileto:**********?subject=意见&cc=***********&bcc=**********">hkhjk</a>Subject 标题cc 抄送bcc 秘抄送九、表格1、基本标记<table width="56%" height="56" border="1" align="center" cellpadding="2" cellspacing="3" bordercolor="#FF00FF" background="file:///C|/Documents and Settings/Administrator/桌面/html/banner资料.jpg" bgcolor="#00FFFF" summary="这是内部资料" > 表格标记<caption align="top"> 标题中华网</caption><tr align="center" valign="middle" bordercolordark="#9900FF" bordercolorlight="#FF0000" > 行标记<th width="50%" height="25"> </th> 单元格表头<td width="50%" nowrap="nowrap" bordercolor="#00FFFF" background="html/banner资料.jpg" bgcolor="#FF00FF" > </td> 单元格标记width 宽height 高nowrap 不换行</tr><tr><td height="26"> </td><td> </td></tr></table><table width="56%" height="84" border="1" cellpadding="2" cellspacing="3" summary="这是中华网的内部资料"> 表格标记<caption align="top"> 说明中华网</caption><tr> 行标记<th width="36%" height="22">dsds</th> 标题单元格标记<td colspan="2">dsddsd</td> 单元格标记（跨列合并）</tr><tr><td height="22">ds</td> height高<td width="19%">sd</td> width 宽<td width="45%" rowspan="2">dsddsd</td> 单元格标记（跨行合并）</tr><tr><td height="22">ds</td><td>sd</td></tr></table>Width 表格宽度border 边宽cellspacing 间距cellpadding 填充bordercolor 边框颜色background 背景图片bgcolor 背景色bordercolorlight 表格左上单元格右下bordercolordark 表格右下单元格左上frame 外部边框显示样式above（上）below（下）border（全显示）hisdes（上下）lhs（左）rhs（右）void（不显示）vsides（左右）rules 内部边框显示样式all（所有）cols（行）groups（行列间）none（不显示）rows（列）十、表单1、<form action="" method="get">具体表单</form> 表单标记2、<label>aa 标签/单行文本<input name="textfield" type="text" value="" size="2" maxlength="4" /> 文本</label><textarea name="textfield2" cols="2" rows="4" wrap="virtual">mmmmmmmmmm</textarea><input name="textfield3" type="password" value="123" size="2" maxlength="3" />Input（单行）：size 字符个数maxlength 最大长度value 初始值type ：text（单行文本）password（密码文本）file（文件域）radio（单选）checkbox（多选）submit（按钮）image（图像域）hidden（隐藏域）button（无）reset（重设）submit（提交）当选组：其实就是把多个当选按钮放在一个表单内标签：<label></label>对表单内容的说明3、textarea（多行）：cols 个数rows 行数wrap 超过处理多行文本4、<select name="menu1" size="1" multiple="multiple" 菜单名size(列表）multiple(允许多选) onchange="MM_jumpMenu('parent',this,0)"> 如果出现代表跳转<option value="">1</option> 菜单项目value如果是跳转代表超链<option selected="selected">2</option> selected（已选中）<option>3</option></select>跳转和列表的区别：都是菜单，不同的是跳转多了一个超级链接的功能十一、框架1、<frameset rows="400,200" cols="*" framespacing="2" frameborder="yes" border="2" bordercolor="#9900FF"> 框架集<frame src="UntitledFrame-2.html" name="topFrame" frameborder="yes" scrolling="No" noresize="NORESIZE" marginwidth="2" marginheight="2" bordercolor="#FF00FF" id="topFrame" /> 框架<frame src="UntitledFrame-3.html" name="bottomFrame" frameborder="no" scrolling="yes" noresize="noresize" id="bottomFrame" />Frameset：rows（第一行框架的高度，第二行的高度）framespacing 边框高度frameborder 是否有边框border 边框大小bordercolor 边框色Frame：src（文件）scrolling（滚动方式）noresize（不能调整大小）marginwidth （边界宽度）marginheight（边界高度）2、<iframe src="file:///C|/Documents and Settings/Administrator/桌面/Untitled-1.html" width="200" height="200" name="22" align="left" frameborder="1" scrolling="yes" marginheight="2" marginwidth="2"></iframe>Src 原文件width 宽height 高name 名称align 对齐frameborder 边框是否显示scrolling 滚动方式marginheight 边缘宽度marginwidth 边缘高度在做框架时，如果想在框架中显示输入target十二、层<div id="Layer1"></div><style type="text/css"></style>十二、CSS（一次套用，多次使用；语法简单；丰富效果）1、定义（外部）.bb（选择符）{ 类font-size（属性）: larger（值）; 声明font-weight: lighter;}Body，h1,h2 { 标签声明内容}#aa { ID声明内容}a:link { 伪标签组声明内容}2、使用（外部）1、附加：<link href="文件路径el="stylesheet" type="text/css" /> 标签类自动生效2、使用：<p class="bb"> hfhfh </p> 类3、套用：<p id="aa">fhfhfhf</p> ID3、定义（内部）<style type="text/css">定义内容</style>或标签中直接使用style属性多个；隔开4、使用（内部）同上如果出现多个定义相同属性，用，隔开5、值时间：1m=1000ms长度：px（像素），pt（点1/72英寸），cm，mm，in（英寸），%颜色：#rrggbb，#rgb，rgb（a，a，a）rgb（a%，a%，a%）url：url（“域名”）6、类型font-family: "宋体"; 字体------类型font-size: 36px; 字号font-style: oblique; 字类型line-height: 20px; 行高font-weight: bolder; 字宽font-variant: normal; 变体text-transform: capitalize; 文本样式color: #000000; 颜色text-decoration: underline overline line-through blink; 大写样式background-attachment: scroll; 附件--------背景background-color: #FF00FF; 背景色background-image: url(html/banner%E8%B5%84%E6%96%99.jpg); 背景文件background-repeat: repeat; 水平位置background-position: left bottom; 垂直位置letter-spacing: 20em; 字母间距------区块text-align: center; 文本对齐text-indent: 2px; 文字缩进vertical-align: baseline; 垂直对齐word-spacing: 10em; 单词间距white-space: nowrap; 空格display: table-row-group; 显示border: 10px 10 10; --------方框margin: 10px; 边界padding: 10px; 填充clear: both; 清楚float: right; 浮动height: 100px; 高width: 100px; 宽border: 20px solid #FF0000 边框（宽度/样式/颜色）-边框list-style-position: outside; 位置----------列表list-style-image: url(html/banner%E8%B5%84%E6%96%99.jpg); 图片list-style-type: square; 类型overflow: scroll; 溢出处理--------定位position: absolute; 类型visibility: visible; 显示z-index: auto; 层数height: 20px; 高width: 20px; 宽left: 10px;top: 10px;right: 10px;bottom: 10px;clip: rect(10px,10px,10px,10px); 裁剪十三、Javascript1、行为原理：先定义一个Javascript函数，在具体对象上应用A、定义<script type="text/JavaScript"> 使用脚本，类型为Javascriptfunction MM_popupMsg(msg) { //v1.0 定义函数，函数名为MM_popupMsg(msg) alert(msg);}</script><a href="#"><img src="html/banner资料.jpg" onclick="MM_popupMsg('本图片禁止下载！')" /></a> 使用函数，时间=“函数名（参数）”。

hjkjhkhjkdfgf

After you．您先。

Allow me．让我来。

Any day will do．哪一天都行夕Any messages for me? 有我的留言吗? Any thing else? 还要别的吗?Any urgent thing？有急事吗？Are you kidding? 你在开玩笑吧!Are you sure? 你肯定吗?As soon as possible! 越快越好!Be careful! 注意!Be quiet! 安静点!Believe it or not! 信不信由你! Between us. 你知，我知。

Big mouth! 多嘴驴！Blast! **！Bless you! 祝福你!Bottle it! 閉嘴！Bottoms up! 干杯！Bottoms up! 干杯(见底)!Boy! （表示惊奇，兴奋等）哇！好家伙！Break the rules. 违反规则。

Can I have this. 可以给我这个吗？Can I help you? 我能帮你吗?Can you dig it？你搞明白了吗？Can－do. 能人。

Cheap skate! 小气鬼！Cheer up! 振作起来!Chin up. 不气，振作些。

Close－up. 特寫鏡頭。

Clothes make the man．人要衣装。

Come on! 快点，振作起来！Come on, be reasonable. 嗨，你怎么不讲道理。

Come on．来吧(赶快)Come seat here. 来这边坐。

Congratulations! 祝贺你!Control yourself! 克制一下!Count me on 算上我。

Daring! 亲爱的！Dead end. 死胡同。

December heartbeat. 黄昏恋。

Did you miss the bus? 你错过公共汽车了? Dinner is on me. 晚饭我请。

香港科技大学实验室安全管理系统

特约专栏香港和美国高校实验室的安全与环保管理编者按:香港和美国高等学校十分重视EH S 管理(env iro nmet 环境,health 健康,safety 安全),安全理念已成为大学核心价值观之一。

学校的高层领导对安全工作的高度重视与鼎力支持,使学校的安全管理工作做到了规范化、制度化和程序化。

本期特约专栏!特请香港科技大学健康、安全及环境处关继祖处长和浙江大学实验室与设备管理处阮慧副处长等,介绍香港和美国部分高校实验室安全与环保管理的经验,供各高校参考。

香港科技大学实验室安全管理系统关继祖,俞宗岱(香港科技大学健康、安全及环境处,香港九龙)摘要:建立有效的实验室安全管理系统,需要校方提供多方面的配套,如行政、人事、技术、资源等支持。

此外,各职别在安全管理的权责要清晰,安全指引要详尽及明确,各项安全服务要周详并有效率,系统效果要定期监察,并要持续改进,才能建立实验室安全文化。

关键词:实验室;安全管理系统;安全文化;香港中图分类号:G 482 文献标志码:A 文章编号:1002 4956(2009)10 0001 03Laboratory safety management system in the HongKong University of Science and TechnologyKWAN Joseph Kai Cho,YU Samuel Chung T oi(H ealth Safety and Environment Office,Ho ng K ong U niversit y o f Science and T echno log y,K ow lo on,H ong Ko ng ,China)Abstract:In or der to establish an effectiv e labo rato ry safety management system,a univ ersity needs to pro vide administr ativ e,per sonnel,techno lo gical and financial suppor ts.In addition,to foster a labo rator y safety cul ture ,ro les and respo nsibilities needs to be clear ly specified,safet y po licies and procedur es precise and co ncise,safety related serv ices co mpr ehensive and effectiv e,perfo rmance fr equently mo nitor ed and operations co nt inu o usly impro ved.Key words:laborat ory ;safety manag ement system;safet y culture;H o ng Ko ng收稿日期:2009 09 01作者简介:关继祖(1954 ),男,香港人,博士,博士生导师,香港科技大学健康、安全及环境处处长,长期在美国及香港从事职安健管理及危害控制研究工作.1 实验室安全为大学整体安全管理之组成部分香港科技大学自1991年成立初期就将安全管理作为所有运作的重要元素,无论在教学、科研及校园设施操作上,均包括一些必须达标的安全管理要求。

Factory Compliance check list -V2 2009

Wage and have implemented it within the specified time period.
工廠必須持有最低工資的最新文件及已在指定期間內實行.
X
rmation on how pay is calculated must be communicated to
11天法定假期(元旦<1>天;春節<3>天;清明節<1>天;勞動節<1>天;端午節<1>天;中秋節<1>天及國慶節<3>天).
X
b. Paid Annual Leave: (Article 45) 1-10 years – 5 days, 11-20 years – 10 days and >over 20 years –15days.
reflect the number of monthly hours to be worked and the
minimum wage.
有否與每一位員工簽署一份明文規定的合同?這個合同必須
能反映每月的工時及最低工資.
X
a.If piece rate is used the method of calculation method must be
X
13. Wage deductions must be specified in facility policy in accordance
with the law and if applicable, approved by the labor union.
必須在工廠的政策內,訂明已符合法規的扣款及如适用者,工資
合同必須獲得當地勞動局的加簽.
X

Phosphatidylcholine Assay Kit 产品说明书

Phosphatidylcholine Assay Kit Catalog Number MAK049Storage Temperature –20 °CTECHNICAL BULLETINProduct DescriptionPhosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in theextracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid.In this assay, PC concentration is determined by acoupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PC present.ComponentsThe kit is sufficient for 100 assays in 96 well plates.PC Assay Buffer25 mL Catalog Number MAK049AFluorescent Peroxidase Substrate, in DMSO0.2 mL Catalog Number MAK049B PC Hydrolysis Enzyme1 vl Catalog Number MAK049C PC Development Mix1 vl Catalog Number MAK049D PC Standard, 10 µmole1 vlCatalog Number MAK049EReagents and Equipment Required but Not Provided.• 96 well flat-bottom plate –It is recommended to useblack plates with clear bottoms for fluorescence assays and clear plates for colorimetric assays.• Fluorescence or spectrophotometric multiwell platereaderPrecautions and DisclaimerThis product is for R&D use only, not for drug,household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Preparation InstructionsBriefly centrifuge vials before opening. Use ultrapure water for the preparation of reagents. To maintain reagent integrity, avoid repeated freeze/thaw cycles.PC Assay Buffer –Allow buffer to come to roomtemperature before use.Fluorescent Peroxidase Substrate –Thaw at roomtemperature to melt the solution prior to use.Aliquot and store protected from light and moisture at –20 °C. Upon thawing, the Fluorescent Peroxidase Substrate is ready-to-use in the colorimetric assay.For the fluorescence assay, dilute an aliquot of the Fluorescent Peroxidase Substrate 5 to 10-fold with PC Assay Buffer, just prior to use. This will reduce the background of the fluorescence assay.PC Hydrolysis Enzyme and PC Development Mix –Reconstitute each with 220 µL of PC Assay Buffer. Mix well by pipetting, then aliquot and store,protected from light, at –20 °C. Use within 2 months of reconstitution and keep cold while in use.PC Standard –Reconstitute in 200 µL of water to0generate a 50 mM (50 nmole/µL) PC Standard solution. Mix well by pipetting until it forms a uniform suspension, then aliquot and store at –20 °C. Use within 2 months of reconstitution and keep cold while in use.Storage/StabilityThe kit is shipped on wet ice. Storage at –20 °C, protected from light, is recommended.2ProcedureAll samples and standards should be run in duplicate. PC Standards for Colorimetric DetectionDilute 10 µL of the 50 mM (50 nmole/µL) PC Standard Solution with 990 µL of water to prepare a 0.5 mM (0.5 nmole/µL) standard solution. Add 0, 2, 4, 6, 8,10 µL of the 0.5 mM PC standard solution into a 96 well plate, generating 0 (blank), 1, 2, 3, 4, and 5 nmole/well standards. Add PC Assay Buffer to each well to bring the volume to 50 µL.PC Standards for Fluorometric DetectionDilute 10 µL of the 50 mM (50 nmole/µL) PC Standard Solution with 990 µL of water to prepare a 0.5 mM (0.5 nmole/µL) standard solution.Dilute 10 µL of the 0.5 mM standard solution with 90 µL of water to generate a 0.05 mM (0.05 nmole/µL). Add 0, 2, 4, 6, 8, 10 µL of the 0.05 mM PC standard solution into a96 well plate, generating 0 (blank), 0.1, 0.2, 0.3, 0.4, and 0.5 nmole/well standards. Add PC Assay Buffer to each well to bring the volume to 50 µL.Sample PreparationTissue (10 mg) or cells (2 ×106) should be rapidly homogenized in 4 volumes of cold PC Assay buffer. Centrifuge at 13,000 ×g for 10 minutes at 4 °C to remove insoluble material.Serum samples may be assayed directly.Bring samples to a final volume of50 µL with PC Assay Buffer.For unknown samples, it is suggested to test several sample dilutions to ensure the readings are within the linear range of the standard curve.Choline present in the sample can generate background. To control for choline background, include a blank sample for each sample by omitting the PC Hydrolysis Enzyme in the Reaction Mix. Assay Reaction1.Set up the Master Reaction Mixes according to thescheme in Table 1. 50 µL of the appropriate Master Reaction Mix is required for each reaction (well). Table 1.Reaction Mixes2.Add 50 µL of the appropriate Reaction Mix to eachof the wells. Mix well using a horizontal shaker orby pipetting, and incubate the reaction for30 minutes at room temperature. Protect the platefrom light during the incubation.3.For colorimetric assays, measure the absorbanceat 570 nm (A570). For fluorometric assays, measure fluorescence intensity (λex= 535/λem= 587 nm).3 ResultsCalculationsThe background for the assays is the value obtained for the 0 (blank) PC Standard. Correct for the background by subtracting the 0 (blank) value from all readings. Background values can be significant and must be subtracted from all readings. Use the values obtained from the appropriate PC standards to plot a standard curve.Note: A new standard curve must be set up each time the assay is run.Subtract the blank sample value from the sample reading to obtain the corrected measurement. Using the corrected measurement, the amount of PC present in the sample may be determined from the standard curve Concentration of PCS a/S v= CS a= Amount of PC in unknown sample (nmole) from standard curveS v= Sample volume (µL) added into the wellsC = Concentration of PC in samplePC molecular weight: 768 g/moleSample CalculationAmount of PC (S a) = 5.84 nmole(from standard curve)Sample volume (S v) = 50 µLConcentration of PC in sample5.84 nmole/50 µL = 0.1168 nmole/µL0.1168 nmole/µL ×768 ng/nmole= 89.7 ng/µL4LS,MAM 09/12-1©2012 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at and/or on the reverse side of the invoice or packing slip.。

企业信用报告_香港RHK亚洲有限公司上海代表处

二、股东信息 .........................................................................................................................................................7 三、对外投资信息 .................................................................................................................................................7 四、企业年报 .........................................................................................................................................................7 五、重点关注 .........................................................................................................................................................8
5.1 被执行人 ......................................................................................................................................................8 5.2 失信信息 ......................................................................................................................................................8 5.3 裁判文书 ......................................................................................................................................................8 5.4 法院公告 ......................................................................................................................................................9 5.5 行政处罚 ......................................................................................................................................................9 5.6 严重违法 ......................................................................................................................................................9 5.7 股权出质 ......................................................................................................................................................9 5.8 动产抵押 ......................................................................................................................................................9 5.9 开庭公告 ......................................................................................................................................................9

港加贺电子（深圳）有限公司介绍企业发展分析报告

Enterprise Development专业品质权威Analysis Report企业发展分析报告港加贺电子（深圳）有限公司免责声明:本报告通过对该企业公开数据进行分析生成，并不完全代表我方对该企业的意见，如有错误请及时联系;本报告出于对企业发展研究目的产生，仅供参考，在任何情况下，使用本报告所引起的一切后果，我方不承担任何责任:本报告不得用于一切商业用途，如需引用或合作，请与我方联系:港加贺电子（深圳）有限公司1企业发展分析结果1.1 企业发展指数得分企业发展指数得分港加贺电子（深圳）有限公司综合得分说明：企业发展指数根据企业规模、企业创新、企业风险、企业活力四个维度对企业发展情况进行评价。

该企业的综合评价得分需要您得到该公司授权后，我们将协助您分析给出。

1.2 企业画像类别内容行业计算机、通信和其他电子设备制造业-其他电子设备制造资质一般纳税人产品服务是：研发、经营电子元器件、微电子线路板组1.3 发展历程2工商2.1工商信息2.2工商变更2.3股东结构2.4主要人员2.5分支机构2.6对外投资2.7企业年报2.8股权出质2.9动产抵押2.10司法协助2.11清算2.12注销3投融资3.1融资历史3.2投资事件3.3核心团队3.4企业业务4企业信用4.1企业信用4.2行政许可-工商局4.3行政处罚-信用中国4.4行政处罚-工商局4.5税务评级4.6税务处罚4.7经营异常4.8经营异常-工商局4.9采购不良行为4.10产品抽查4.11产品抽查-工商局4.12欠税公告4.13环保处罚4.14被执行人5司法文书5.1法律诉讼（当事人）5.2法律诉讼（相关人）5.3开庭公告5.4被执行人5.5法院公告5.6破产暂无破产数据6企业资质6.1资质许可6.2人员资质6.3产品许可6.4特殊许可7知识产权7.1商标信息最多显示100条记录，如需更多信息请到企业大数据平台查询7.2专利7.3软件著作权7.4作品著作权7.5网站备案7.6应用APP7.7微信公众号8招标中标8.1政府招标8.2政府中标8.3央企招标8.4央企中标9标准9.1国家标准9.2行业标准9.3团体标准9.4地方标准10成果奖励10.1国家奖励10.2省部奖励10.3社会奖励10.4科技成果11 土地11.1大块土地出让11.2出让公告11.3土地抵押11.4地块公示11.5大企业购地11.6土地出租11.7土地结果11.8土地转让12基金12.1国家自然基金12.2国家自然基金成果12.3国家社科基金13招聘13.1招聘信息感谢阅读:感谢您耐心地阅读这份企业调查分析报告。

凯斯克 2件后重dapshield与斑马车JK Wrangler 2007-2019年款的兼容性谱说

Your Quadratec® branded accessories are covered by the following Limited Warranty provided exclusively by Quadratec, Inc., 1028 Saunders Lane, West Chester PA 19380.
©Quadratec, Inc. 2019. All Rights Reserved. 4.22.19 A4 Version
Part #12999.3134
®
The Trusted Source
4
This Limited Warranty gives you specific rights. You may also have other rights that vary from state to state. For example, some states do not allow limitations of how long an implied warranty lasts and /or do not allow the exclusion or limitation of incidental or consequential damages, so the limitations and exclusions herein may not apply to you.
All other warranties are hereby disclaimed, except to the extent prohibited by applicable law in which case any implied warranty of merchantability or fitness for a particular purpose on this product is limited to 2 year from date of initial retail sale. Quadratec reserves the rights to: (a.) require invoice or other proof your accessory is within the terms of this Limited Warranty as a condition of warranty service and, (b.) make future revisions to this product and Limited Warranty without prior notice or obligation to upgrade your product.

香港赫赫国际集团有限公司(企业信用报告)- 天眼查

考。
1.3 变更记录
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1.4 主要人员
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4.4 企业业务
截止 2018 年 09 月 11 日，根据国内相关网站检索及天眼查数据库分析，未查询到相关信息。不排除因信息公开来源尚未公开、公开形式存在差异等情况导致的信息与客观事实不完全一致的情形。仅供客户参考。
5

4.5 竞品信息
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五、风险信息
5.1 被执行人信息
截止 2018 年 09 月 11 日，根据国内相关网站检索及天眼查数据库分析，未查询到相关信息。不排除因信息公开来源尚未公开、公开形式存在差异等情况导致的信息与客观事实不完全一致的情形。仅供客户参考。
5.2 失信信息
截止 2018 年 09 月 11 日，根据国内相关网站检索及天眼查数据库分析，未查询到相关信息。不排除因信息公开来源尚未公开、公开形式存在差异等情况导致的信息与客观事实不完全一致的情形。仅供客户参考。
4.2 投资事件
截止 2018 年 09 月 11 日，根据国内相关网站检索及天眼查数据库分析，未查询到相关信息。不排除因信息公开来源尚未公开、公开形式存在差异等情况导致的信息与客观事实不完全一致的情形。仅供客户参考。

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全体兄弟、姐妹、朋友们，大家好！首先让我们祝贺自己的狼王养兔联盟顺利开始，大家也有幸一起牵手起来，共同打造一个品牌，创造属于自己的财富。

我们相信自己能够做好，联盟一定可以成功，为自己带来可观的收益。

当然这需要我们的努力和奋斗！我想很多联盟兔场的负责人都有些模糊，有些疑惑。

不知道加盟了联盟兔场能为自己带来什么好处，也不知道联盟兔场将如何给自己带来效益，不知道加盟兔场要做些什么事情有什么义务。

在下面我想就这些问题，给大家详细分析。

第一、养兔行业的商机凸显并且巨大：正如我在联盟成立的背景中所说的，当前这个养兔行业极其混乱。

很多的兔业公司和老养兔人都在忽悠新养殖户，在引种这块尤其黑暗。

用普通得不能再普通的商品兔子充当良好的种苗高价出售给新手们。

牟取了极大的利润。

而且我们很多的联盟兔场在刚刚开始时候也是有被忽悠的经历。

毫无疑问的是养兔行业引种这方面有着极大的商机，利润巨大。

而且每个地方每年都会有很多需要进入养兔行业的人，他们需要数量很大的种兔，连同笼具设备、饲料、药品。

还有的是技术服务、指导。

他们现在进入这个行业是小心翼翼、胆颤心惊，总是担心被无良的养兔企业蒙骗，买到高价低质的兔子，假药、设计不合理的笼子等等。

正因为有着巨大的利益诱惑和大多新手对养兔行业的不了解，于是各个地方都是出现了很多高价倒种，坑蒙新手的兔业公司。

他们上骗国家财政，下骗普通养兔人。

得到了巨大的利益。

行业虽然黑暗，但是商机巨大。

那些骗子公司可以获得巨大的利益，同样的我们为什么不可以呢？军事学上有一个经典的谋略是反其道而行之，今天运用到我们养兔行业同样可以行得通。

当他们都是在靠欺骗手段获得利益的时候，我们可以用绝对的信誉，良好的质量，合理的价格去获得。

把他们的生意抢夺过来。

或者分一部分来。

不管怎么说，首先我们要明白的是养兔行业的商机巨大。

这里说的商机不是说靠养殖的出售，是特别指出售种兔、设备、药品、饲料。

技术服务、兔肉销售等等。

第二、抓住商机、正确的理解商机和我们要做的事情。

上面说到了养兔行业的商机巨大，是说出售种兔、饲料、笼具、药物、技术服务、兔肉销售那些方面。

这是时候也许有人要说了，那我们不是等于炒种了么。

也和他们那些兔业骗子一样了么。

或者很多的养兔人会说我们的养兔联盟是专门炒种的组织。

在现在的养兔行业，因为名声早已臭坏，被那些无良奸商破坏了。

所以大家一提到卖种兔，卖设备就是厌恶、恶心、就认为是不应该，好像是骗子才做的行为。

但是事实是那么的吗，完全不是。

很简单的，任何一个新手进入养兔行业都是需要引种的、需要购买设备的、需要技术学习的。

这是行业很合理、正确的市场需求。

谁都可以去做。

只要有实力去做。

如果我们这些存有良知的养兔人不去做不去抓住机遇，那么这个商机就会被那些无良的兔业骗子公司做去。

他们用高价倒卖低劣的兔子给养殖户、出售高价低劣的笼子、甚至假药。

或者是骗取设备货款不发货。

这是因为他们的所作所为，使得这个行业的正确需求交易变味了。

就好比以前的人们一说到发廊就联想到色情场所、一提到小姐就以为是妓女一样。

所以我们的养兔行业也变成了一提到卖种兔就以为是炒种一样。

这其实是很不应该的。

如果我们用真诚的服务、合理的价格、可靠的质量去为新养殖户提供种兔，设备、药品、饲料等等，我们就可以合情合理合法的挣取合适的利润。

获得我们和养殖户、行业的三赢。

我们不是靠骗来获得利益。

记得在国内某著名养兔论坛，有人说了那么一段话什么山寨专家啦-鱼汛之类的；养兔老师啦：狼王这些，都是利用东兔论坛这个平台使劲的炒作自己，然后寻求自己的经济利益-办养兔论坛，卖药，卖兔饲料，卖养兔设备，卖养兔书，办培训班收学费，卖种兔等等。

在这里我想举一个具体的卖种例子给大家：今年的4月3号，我以85一只5.7斤的价格、65一只4斤的价格给广东惠州博罗县的养殖户殷锡杰（联盟论坛注册名字是惠州老弟）提供了50只种兔。

他是正月十七的时候先来我家看过兔子了。

65一只4斤的兔子。

先说价格方面，我当地的肉兔批发价格是10一斤，我出售给周边村民喜宴用兔是12一斤零售。

4斤重的普通肉兔是批发40到45之间（吃饱才出售的）零售的话是50一只左右。

但是经过我断奶开始挑选培养出来兔子我才卖65一只。

就是一只兔子是挣了15的利润吧。

这个价格对我来说很合适。

把好兔子卖了好的价格。

对引种的殷锡杰老弟来说，才多花费了15块就得到了质量很好信誉可靠的兔子。

他也很喜欢。

是双赢的局面。

这时有人会说了，谁知道你的兔子是好还是坏。

呵呵，这其中出现的小插曲可以用绝对的事实征服任何人包括引种户。

当时我们说好是要4月1、2号来取兔子的。

我也没有收取任何定金。

在3月27号时候，殷锡杰兄弟给我来电话，说请求老哥把取兔子的时间推迟20天到4月20号。

那时候可以增加价格给我。

我很奇怪，追问之下才知道他家里出了点事情，钱不够买兔子。

需要等4月20号发工资了才有钱过来。

我当时问他缺多少，他说最多才1500给我。

我考虑了下，说不要推迟，你直接过来拿兔子。

钱不够就先欠着。

等20号发了工资再给我。

当时殷老弟都不敢相信，他给我保证等20号工资一发就给我汇款。

就那么的，我给殷兄弟提供了塑料笼子（帮忙购买的运输笼）、饲料60斤、兔子共计4400元。

但是我只收取了1300元。

剩余的都先欠着。

种兔经过8个小时运输回家以后，无一例出现意外伤亡。

应激都不会。

每个兔子精心挑选培养。

5.7斤重的已经可以直接配种的，现在已经产了10窝。

产子数比我家的还高。

每窝在9个以上（这和配种有关系）。

4月23号殷兄弟给我汇了1500，剩余的1600等5月20号再汇。

我想说的是，朋友们，如果我不是对自己的质量完全放心，绝对可靠。

我能那么的做么？如果出现运输死亡厉害、回家一看兔子都不行。

呵呵，我还想要那剩余的钱么？在他预定种兔时候我没有收取定金，兔子引回去了两月才能给我汇清钱数。

我甚至连他家住哪里都不知道。

朋友们，这样的种兔质量和服务，是炒种行为么？有哪个供种兔场敢和我一样的做。

钱都不要付就可以引种走。

在今天在这里，我需要用自己的实际例子来反驳，并解释我们以后的养兔联盟要如何去提供种兔给别人。

当然不是说都要不给钱就拿兔子走。

只是想说明的是我们给对方提供良好的兔子，合理的价格。

优质的服务，我们就是可以理直气壮的对任何人任何场合宣布卖种、销售设备、饲料都可以。

我们卖了种苗给别人，人家还要谢谢我们给他提供了那么好的兔子，价格优惠服务周到，还会为我们宣传：这个兔场不错！可信！第三、如何去抓住商机，分得蛋糕，发展自己！在上面我们已经知道了行业存在的极大商机，而且也理解了我们要做的事情。

那么我们该如何去抓住商机，使得我们得到确实的利益和发展呢？在家等是等不来机会的。

机会总是留给有准备的人！现在让我们来看看现在的某些兔业骗子们是如何利用商机、实现收益的。

首先在兔场他们会给自己起个很好听的名字，组成公司或者合作社的名誉。

他们会搞得象那么回事情。

会议室、培训室、经理办公室、药品室、仓库等等。

铝合金制作的精美招牌着实的吸引人，各种政府颁发的奖章称号一应俱全。

厂房数量一栋栋排开。

看起来养殖规模极大。

然后呢，很多还有自己的网站，在网络上花费大量的钱进行大势宣传。

依托于电视媒体网络进行不断的广告。

对于养兔新手来说呢，他们不了解啊。

有一部分人呢就是看了电视或者路边的广告牌，被吸引去了。

而大多数年轻人想进入养兔行业的时候呢，他们会上网去查些资料。

于是他们就被那铺天盖地广告吸引住了。

于是在还对兔业一无所知的情况下，被忽悠上当。

从某些兔业骗子的行为和手段中，我们可以看到他们是利用资金优势、扩充规模、做媒体广告宣传、利用很好的门面工作来吸引诱惑新养殖户。

当然这也是有风险的，不是每个兔业骗子那么的做都可以成功。

但是获得利益是极大的。

再回来看我们自己每个兔场，我们大部分人是没有太多资金的。

十几数十万以上的投资都拿不出来，更别说去投资大型兔业公司，上挣取财政补贴，下坑新手朋友。

而且我们也不会那么的做蒙骗新手朋友们，我们都是老实本分的养兔人。

换句话说，即使我们当中的某些人也想那么的去弄，但是投资了还不一定能行骗成功！我们痛恨这些黑幕，但是我们无力改变。

在这之前我们除了呐喊一声以外别无行动。

我们试想，假使有一种方法可以使得我们自己的名声在外，为当地的朋友所知道熟悉、并且信任自己。

而且又不要花费什么资金的。

那不是很好么？即使每年没有获利那么高，但是也是能很好的壮大自己。

而且没有任何风险。

无需投入资金。

就能获得可观的回报。

这是很好的事情啊。

在现在之前想起来，每个人都会说你一定疯了。

怎么可能做到呢？要让人家知道自己的兔场，就必须做广告，广告就是花费巨大。

这是毫无疑问的！但是通过分析，我们已经知道了要抓住商机，就必须做广告，使得周边地区的大部分想养兔的人都知道自己，从而获得收益。

这是条件。

也是问题的关键所在。

因为我们每个兔场没有那么大的实力、资金、规模去吸引人前来你的兔场。

哪怕自己的兔子再好，不去蒙骗人，价格合理便宜，技术好都没有用，因为新手朋友不会知道你的兔场存在。

即使认识了你，也会疑惑不信任你。

因为骗子的门面工作很好，容易叫他们信任。

所以现在开始，我们有了一种全新的模式，就可以实现每个兔场不需要花费什么钱财就能很好的宣传自己的途径和做法。

百益而无一害！这就是我们正在联合做的狼王养兔联盟！通过这个组织、通过我们的努力就能实现这个目标！实现参与分享兔业这块大蛋糕的目的！第四、为什么要组建狼王养兔联盟？我们都知道了每个兔场要分得这块蛋糕，就必须宣传自己。

让人们知道自己的兔场。

但是这需要极大的投资。

还不一定能成功！我们每个人不可能都建立自己的网页，都花费很多钱去搞宣传。

没有资金也没有必要。

单独的力量总是有限的，团队的力量是巨大的。

如果我们联合起来做一件事情呢？那会是什么概念？答案是不需要解说的。

这也就是我们为什么要组织成狼王养兔联盟的目的。

我们需要全国各地的养兔人一起携手，并肩作战，一同奋斗！我们无需要数十个个人养兔网，只需要有一个共同的网站，属于我们每个人自己。

我们共同的去做一件事情，打造我们在养兔行业的响亮品牌。

举个现实的例子：比如我们的狼王养兔联盟在大家的共同宣传和努力下，出名了。

大家都知道了。

网站的点击率位居行业网站的前列时候，我们就实现了每个加盟兔场的宣传效果。

收益就自然的会来。

很多的加盟兔场也许不了解，联盟的出名和信誉对自己有什么宣传效果，有什么立竿见影的宣传意义。

在这里我想先用餐饮行业的连锁企业肯德基来举例，有了实在的例子会比较好对比。

我们都知道肯德基是全球餐饮的巨头之一。

品牌很大。

知名度很高。

我们大家在城市里头如果想去吃饭时候或者请朋友吃好一点的东西，都会习惯的说：“走去肯德基。

”或者你去任何一个城市，你都不知道肯德基在哪里。

但是这并不妨碍我们吃肯德基。

因为我们知道肯德基、麦当劳这类的店一定是在城市最繁华的地段，而且每个地级市都会有。