碱性成纤维细胞生长因子重组腺病毒转染兔骨髓间充质干细胞的表型变化

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碱性成纤维细胞生长因子对体外培养的兔骨髓基质细胞生长特性的影响

碱性成纤维细胞生长因子对体外培养的兔骨髓基质细胞生长特性的影响王志勇;陈增海;于胜吉【期刊名称】《山东大学学报：医学版》【年(卷),期】2006(44)11【摘要】目的:研究骨髓基质细胞(BMSC)在体外培养条件下分化为成骨细胞的能力,及碱性成纤维细胞生长因子(bFGF)对其贴附、增殖、分化的影响。

方法:将各组BMSC传代后加入bFGF,其浓度分别为0、5、10、50、1002、00 ng/ml,用倒置相差显微镜、电镜、四唑盐比色法、碱性磷酸酶(ALP)染色、钙染色和电子探针等方法观察其形态、贴附、生长增殖活性、分化成熟和体外钙化的状况。

结果:经10 ng/ml bFGF诱导后,BMSC贴附细胞数量明显增加。

当100 ng/ml时,bFGF对BMSC的促增殖作用达到最大值。

当加入bFGF时,ALP染色阳性率降低,当bFGF 浓度为100μg/L时阳性率最低(P<0.01)。

传代细胞连续培养30 d后钙染色结果呈阳性,电子探针检测矿化区的Ca/P值与羟基磷灰石结晶中Ca/P值相符。

电镜观察可见培养的细胞具有典型的成骨细胞形态特征,并能产生胶原纤维。

结论:BMSC 在体外培养条件下具有分化为成骨细胞的能力,bFGF能够促进细胞的贴壁和增殖,但对其分化成熟具有抑制作用。

【总页数】5页(P1090-1094)【关键词】骨髓基质细胞;成骨细胞;成纤维细胞生长因子2;兔【作者】王志勇;陈增海;于胜吉【作者单位】山东大学齐鲁医院急诊科;山东大学第二医院骨科;中国医学科学院肿瘤医院骨科【正文语种】中文【中图分类】Q954.658【相关文献】1.不同浓度碱性成纤维细胞生长因子对兔骨髓基质细胞的影响 [J], 韦永中;范卫民;陈哲峰;崔维顶2.重组人骨形态发生蛋白2与碱性成纤维细胞生长因子联合促进兔骨髓基质细胞血管内皮细胞生长因子表达及其成骨潜能 [J], 常祺;黄昌林;黄涛3.兔骨髓基质细胞与陶瓷样异种骨的体外培养实验兔骨髓基质细胞形态、生长、附着及增殖性能观察 [J], 李彦林;韩睿;黄河;李世和;曾才铭;王宏邦4.碱性成纤维细胞生长因子对兔骨髓基质细胞生物行为的影响 [J], 刘建;常祺;胡蕴玉;孟国林5.复合骨形态发生蛋白2及碱性成纤维细胞生长因子注射型骨修复材料对兔骨髓基质细胞增殖和超微结构的影响 [J], 崔赓;胡蕴玉;雷伟;孙明林;李洁;靳小兵;汪培铭;杨柳;吕昌伟;吕荣因版权原因，仅展示原文概要，查看原文内容请购买。

重组人碱性成纤维细胞生长因子对兔视网膜缺血再灌注损伤的保护作用

重组人碱性成纤维细胞生长因子对兔视网膜缺血再灌注损伤的保护作用邵宏超;葛嫣然;李利艳;王林洪;马建英;曹凤英;田华【期刊名称】《临床误诊误治》【年(卷),期】2014(000)006【摘要】目的探讨重组人碱性成纤维细胞生长因子( recombinant human basic fibroblast growth factor, rh-bFGF)对兔视网膜缺血再灌注损伤( retinal ischemia reperfusion injury, RIRI)的保护作用。

方法选取健康纯种大耳白兔66只,随机分为假手术组6只,RIRI组30只,rh-bFGF组30只。

通过升高眼压的方法制作兔RIRI模型,RIRI组予0.9%氯化钠注射液,rh-bFGF组予rh-bFGF治疗,假手术组仅用5号头皮针从耳前方角巩膜缘内刺入前房,之后缓慢拔除针头。

RIRI组和rh-bFGF组在RIRI后6 h、12 h、24 h、48 h、72 h常规HE染色观察视网膜组织形态变化,SABC免疫组织化学法检测视网膜组织中凋亡抑制基因Bcl-2和促凋亡基因Bax蛋白表达及Bcl-2/Bax的变化。

结果假手术组视网膜未见凋亡细胞；RIRI组视网膜凋亡细胞主要出现在内核层和神经节细胞层,且在24 h达到高峰；rh-bFGF组与RIRI组比较,观察各时间点细胞损伤情况基本相似。

Bcl-2和Bax在正常视网膜组织中几乎不表达,在RIRI后6 h开始表达,24 h达到高峰,48 h开始下降,72 h后表达明显减弱。

rh-bFGF组与RIRI组比较,Bcl-2表达明显增强,而Bax 表达减弱(P均<0.05)。

结论 Bcl-2和Bax参与了RIRI的调控,rh-bFGF可通过上调Bcl-2表达及下调Bax表达,使Bcl-2/Bax比值升高,起到保护视网膜组织的作用。

%Objective To explore the protective effect of recombinant human basic fibroblast growth factor (rh-bFGF) on retinal ischemia-reperfusioninjury (RIRI) in rabbits. Methods The 66 healthy purebred rabbits were randomly divided into sham-operation group (n=6), ischemia-reperfusion model group (n=30) and rh-bFGF treatment group (n=30). The RIRI models were established by elevating intraocular pressure, the ischemia-reperfusion model group was injected with 0. 9% sodium chloride injec-tion;rh-bFGF drug therapy was performed in rh-bFGF treatment group;the sham-operation group only received the 5th scalp heedle from ear piercing the anterior chamber angle in front of the sclera edge, and then the needle was slowly removed. Retina histopatho-logical changes were observed by routine hematoxylin and eosin stain 6 h, 12 h, 24 h, 48 h and 72 h after RIRI in ischemia-reperfu-sion model and rh-bFGF treatment groups. Expressions of inhibiting gene Bcl-2 and stimulative gene Bax and changes of Bax/Bcl-2 in retinal tissues were detected with SABC immunohistochemical method. Results No apoptotic cell was found in sham-operation group;apoptotic cells mainly existed in the inner nuclear layer and the ganglion cell layer, and apoptotic cells reached the peak 24 h after reperfusion;the rh-bFGF treatment groups had the similar condition with the ischemia-reperfusion model group in cell in-jury at each observational time. No expressions of Bcl-2 and Bax positive cells were found in normal retina tissues;the expressions were found 6 h after reperfusion, and the expressions reached the peak 24 h after reperfusion, and began to descented 48 h after reperfusion, and the decrease was obvious 72 h after reperfusion. Bcl-2 expression was significantly stronger, while Bax expression was significantly reduced in rh-bFGF treatment group,compared with those in ischemia-reperfusion model group (P<0. 05). Con-clusion Bcl-2 and Bax protein are involved in the RIRI regulation. The retina tissue can be protected with Bcl-2 expression of up-regulation and the Bax expression of down-regulation by rh-bFGF, which can increase the Bcl-2/Bax ratio.【总页数】5页(P100-104)【作者】邵宏超;葛嫣然;李利艳;王林洪;马建英;曹凤英;田华【作者单位】063000 河北唐山，河北联合大学附属医院眼科;063000 河北唐山，河北联合大学附属医院眼科;100000 北京，石景山区医院眼科;063000 河北唐山，河北联合大学附属医院眼科;063000 河北唐山，唐山市第九医院门诊部;063000河北唐山，河北联合大学附属医院眼科;063000 河北唐山，河北联合大学附属医院眼科【正文语种】中文【中图分类】R322.91【相关文献】1.神经生长因子联合银杏叶提取物对兔急性青光眼视网膜缺血再灌注损伤的保护作用 [J], 李岳美;李庆和;郑新华2.rh-bFGF 对兔视网膜缺血再灌注损伤的保护作用及其机制 [J], 邵宏超;葛嫣然;刘岩;苏杰;田华3.rh-bFGF对兔视网膜缺血再灌注损伤的保护作用及机制 [J], 邵宏超;葛嫣然;刘岩;苏杰;田华4.热休克蛋白72在兔视网膜缺血再灌注损伤中的表达及重组人碱性成纤维细胞生长因子对其表达的影响 [J], 邵宏超;葛嫣然;刘岩;苏杰;杨馥宇5.活血通络利水方对兔视网膜缺血/再灌注损伤的保护作用 [J], 杨赞章; 张越; 张铭连; 孟兢晶; 李明然因版权原因，仅展示原文概要，查看原文内容请购买。

腺病毒介导的碱性成纤维细胞生长因子对大鼠脊髓损伤腹后外侧索少突胶质细胞的影响

腺病毒介导的碱性成纤维细胞生长因子对大鼠脊髓损伤腹后外侧索少突胶质细胞的影响杨彦玲【摘要】Objective To study the effect of adenovirus-mediated basic fibroblast growth factor ( FGF-2) gene transfer in vivo on oligodendrocyte cell numbers throughout ventrolateral white matter following spinal cord injury in rats. Methods Thirty-two adult female Sprague Dawley rats were injured with the Infinite Horizon Impactor, and then were randomly assigned to four groups; FGF-2-Adts high-titer group (1. 27 × 10 pfu/rat) , FGF-2 -Adts intermediate-titre group (6. 37 × 106 pfu/rat) , FGF-2-Adts low-titer group (3. 18 × 106 pfu/rat) , and green fluorescent protein (GFP) -Adts group (5. 9 × 107 pfu/rat). The transgenic expression in vivo was detected with fluorescence microscopy. The locomotor function of the hindlimbs of rats was evaluated using Riv-lin plate. Slides mounted with tissue sections were processed for immunohistochemical detection and quantification of oligodendrocytes (CC1 + ) in the ventral lateral funiculi (VLF) of injured spinal cords. Results One week after spinal cord injury, GFP showed that many cells had expressed objective gene in vivo and the angles of the occlusal plane of rats in FGF-2 groups were significantly higher than in GFP-Adts group. Also, there was a significant difference among the FGF-2-Adts treatment groups for the volume of gray matter sparing. However, there were no significant differences for total white matter sparing. Stereological quantification of total CC1 * cell numbers inthe spared VLF showed a significant reduction in numbers with GFP controls compared to all other groups 4 weeks after injury. In contrast, the FGF-2 Adts intermediate-titer group had significantly more CC1 + cells when compared to both the FGF-2-Adts high- and low-titer groups. Conclusion Adenovirus-mediated FGF-2 gene transfer can promote the functional recovery of the injured spinal cord by enhancing the proliferation and/or differentiation of oligodendrocytes.%目的观察腺病毒( Adts)介导碱性成纤维细胞生长因子(FGF-2)对大鼠脊髓损伤腹后外侧索少突胶质细胞的影响.方法 32只SD大鼠在T10水平制备脊髓损伤模型,随机分为体内转基因治疗组和损伤对照组,用携带FGF-2和绿色荧光蛋白(GFP)的Adts行直接体内转基因治疗.荧光显微镜观察体内转基因表达,斜板实验检测大鼠运动功能恢复情况,免疫组织化学染色观察腹后外侧索少突胶质细胞(CC1+)数量的变化.结果荧光显微镜观察发现,Adts载体直接引入脊髓后可有效感染脊髓组织并表达报告基因.斜板实验检测结果显示,体内转基因治疗组大鼠的倾斜平面角度明显高于对照组(P＜0.05或P＜0.01).免疫组织化学结果证实,腹后外侧索CCI+数量较对照组明显增加(P＜0.05).结论 Adts介导的FGF-2可促进少突胶质细胞的存活和增殖.【期刊名称】《中国医学科学院学报》【年(卷),期】2012(034)004【总页数】5页(P348-352)【关键词】脊髓损伤;碱性成纤维细胞生长因子;少突胶质细胞;腺病毒载体;基因治疗【作者】杨彦玲【作者单位】延安大学医学院生理教研室,陕西延安716000【正文语种】中文【中图分类】R744脊髓损伤是中枢神经系统的严重创伤，因其高致残率和高病死率一直成为医学研究的热门课题。

转化生长因子β3基因转染兔骨髓间充质干细胞诱导其向软骨表型的分化

中国组织工程研究与临床康复第15卷第27期 2011–07–02出版Journal of Clinical Rehabilitative Tissue Engineering Research July 2, 2011 Vol.15, No.27P.O. Box 1200, Shenyang 110004 50481Department of Joint Surgery, 2Department of Rehabilitation, 3Central Laboratory, Affiliated Hospital, Qingdao University Medical College, Qingdao 266000, Shandong Province, ChinaSui Lai-jian ★,Master, Department of Joint Surgery, Affiliated Hospital, Qingdao Univ ersity Medical College, Qingdao 266000, Shandong Province, ChinaCorrespondence to: Wang Ying-zhen, Doctoral supervisor, Department of Joint Surgery, Affiliated Hospital, Qingdao University Medical College, Qingdao 266000, Shandong Province, China wangy ingzhenqd@ Supported by: the Science andTechnology Tackle Key Plan of Qingdao City, No.06-2-2-6- nsh-2*Received: 2011-01-03 Accepted: 2011-02-24转化生长因子ß3基因转染兔骨髓间充质干细胞诱导其向软骨表型的分化*★隋来健1，吕成昱1，张海宁1，徐浩1，高正玉2，隋爱华3，王英振1Differentiation of rabbit mesenchymal stem cells into cartilage phenotype induced by transforming growth factor beta 3Sui Lai-jian 1, Lü Cheng-yu 1, Zhang Hai-ning 1, Xu Hao 1, Gao Zheng-yu 2, Sui Ai-hua 3, Wang Ying-zhen 1AbstractBACKGROUND: Endogenous induction of cartilage differentiation is to use a certain carrier to integrate the target genes into stem cells to induce self-differentiation by self-induced secretion of cytokines.OBJECTIVE: To observe the ability of rabbit bone marrow mesenchymal stem cells (BMSCs) transfected with transforming growth factor beta 3 (TGF-ß3) to differentiate into cartilage phenotype through adeno-associated virus vector.METHODS: The third passage BMSCs in logarithmic growth phase was transfected by rAAV2-TGF-ß3. Total protein was extracted from cells at 3, 6, 9, 12 days after transfection, and TGF-ß3 was detected through ELISA. After 2 weeks of culturing, mRNA expressions of type Ⅱ collagen were determined by RT -PCR and the collagen Ⅱ protein was detected by western blot. The expression of proteoglycan was shown by toluidine blue staining.RESULTS AND CONCLUSION: After transfection, BMSCs can express TGF-ß3 stably, and make a better cartilagetransformation compared to negative control group. TGF-ß3 can be transfected into BMSCs through adeno-associated virus vectors and induce cell differentiation into cartilage phenotype.Sui LJ, Lü CY, Zhang HN, Xu H, Gao ZY, Sui AH, Wang YZ. Differentiation of rabbit mesenchymal stem cells into cartilage phenotype induced by transforming growth factor beta 3.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(27): 5048-5052. [ ]摘要背景：内源性诱导软骨分为就是通过一定的载体将目的基因整合入干细胞内，使其自行分泌诱导因子诱导自身进行分化。

碱性成纤维细胞生长因子重组腺病毒转染兔骨髓间充质干细胞的表型变化

碱性成纤维细胞生长因子重组腺病毒转染兔骨髓间充质干细胞的表型变化蔡弢艺;陈雄生;贾连顺;孙延卿;林斌;陈长青【期刊名称】《中国组织工程研究》【年(卷),期】2014(000)023【摘要】BACKGROUND:Exogenous basic fibroblast growth factor (bFGF) plays an important role in the ligament tissue healing process, and the use of transgenic methods to transfect exogenous genes into cells can promote the secretion of bFGF. OBJECTIVE:To observe phenotypic changes and the bFGF protein expression after bFGF recombinant adenovirus was used to transfect rabbit bone marrow mesenchymal stem cells (BMSCs). METHODS:Passage 2 BMSCs were divided into three groups:Ad.bFGF-eGFP group, Ad.eGFP group and control group. Under a phase contrast microscope we observed the changes in cellmorphology. The expression of bFGF protein in BMSCs was determined by enzyme-linked immunosorbent assay (ELISA). The proliferative curve was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). RESULTS AND CONCLUSION:The transfected cells showed a uniform phenotype of fibroblasts. MTT colorimetric assay revealed that more proliferative activity of transfected BMSCs was shown in the Ad.bFGF-eGFP group than in the Ad.eGFP group and control group. ELISA results showed that expression of bFGF protein was higher in the Ad.bFGF-eGFP group than inthe Ad.eGFP group and control group (P<0.05). BFGF recombinant adenovirus can induce the differentiation of BMSCs into fibroblasts, increase proliferative ability and promote the expression of bFGF protein.%背景：外源性碱性成纤维细胞生长因子在韧带组织的愈合过程中发挥着重要作用，采用转基因方法将外源性基因转入细胞内能促进碱性成纤维细胞生长因子分泌。

碱性成纤维细胞生长因子及内皮细胞生长因子对兔韧带细胞增殖的影

【】目的观察碱性成纤维细胞生长因子bFGF和内皮细胞生长因子ECGF对内侧副韧带MCL和前十字韧带ACL细胞增殖行为的影响。方法培养10周龄新西兰白兔内侧副韧带和前十字韧带细胞，在培养液中分别加入bFGF和ECGF，以XTT方法测定细胞的增殖行为。结果 bFGF在1 ngml时即对MCL细胞有显著的促增殖作用，5 ngml时开始对ACL细胞有促进作用，其浓度达50 ngml时，对两种细胞的促进作用最大。ECGF在3.125 ngml时即对MCL细胞有显著的促增殖作用，其浓度达12.5 ngml时对ACL细胞有促进作用。bFGF和ECGF在超过其最佳作用浓度后，随浓度升高促进作用不再增强。结论 bFGF和 ECGF可以促进韧带细胞增殖进而促进韧带创伤愈合。【关键词】碱性成纤维细胞生长因子;内皮细胞生长因子;韧带;细胞增殖【Abstract】 Objective To observe the effects of basic fibroblast growth factor bFGF and endothelial cell growth factor ECGF on the proliferation of the cells from medial collateral ligament MCL and anterior cruciate ligament ACLdthods The MCL and ACL cells of tenweekold skeletally immature New Zealand white rabbit were cultured, while bFGF and ECGF were added to the cell culture media, the cellular proliferation was assayed by XTT method.Results The addition of 1 ngml bFGF enhanced the proliferation of MCL cells significantly, 5 ngml bFGF enhanced the proliferation of ACL cells ,this enhancement of proliferation of both MCL cells and ACL cells was maximal in the concentration of 50 ngml. The adition of 3.125 ngml ECGF enhanced the proliferation of MCL cells significantly, 12.5 ngml ECGF enhanced the proliferation of ACL cells.The efficacy of bFGF and ECGF declined gradually when they present at concentrations greater than the dose with the greatest efficacy.Conlusion It is evident that bFGF and ECGF can enhance the proliferation of the ligamentous cells and they can enhance the healing of ligamentous issue 毕业论文网【Key words】 bFGF, ligament,proliferation,ECGF 韧带损伤是膝部的常见运动创伤，尤以内侧副韧带medial collateral ligament,MCL和前十字韧带anterior cruciate ligament,ACL多见，MCL和ACL是维持膝关节稳定的重要韧带，其损伤可使膝关节丧失稳定性，引起功能障碍，甚至导致骨关节炎[21]。在中央实质部完全断裂后，MCL可自然愈合，有时不需手术介入;而ACL损伤后，即使手术缝合断端，其愈合率也较低。韧带损伤后的愈合过程与一般结缔组织相似，都经过急性炎症期、修复再生期和重塑或成熟期三个阶段[2]。研究表明[3]，在损伤韧带的局部存在着多种生长因子，它们被认为是韧带正常愈合过程的重要调节者。使用生长因子促进韧带愈合是近年来研究的焦点之一。作者通过体外培养MCL和ACL细胞，分别加入碱性成纤维细胞生长因子bFGF和内皮细胞生长因子ECGF，以观察bFGF和ECGF对韧带细胞增殖的影响，为应用bFGF和ECGF促进韧带创伤愈合提供依据。 1 材料与方法 1.1 主要试剂 bFGF暨南大学生物医学工程研究所惠赠，ECGFSigma公司产品，美国，DMEM培养基及胰蛋白酶Gibco公司产品，胶原酶Ⅱ、XTTSigama公司，美国，胎牛血清杭州四季青生物有限公司,酶标仪日本Reader。毕业论文网 1.2 内侧副韧带和前十字韧带成纤维细胞培养无菌切取兔龄10周新西兰白兔MCL和ACL，在含100 μgml PG和100 μgml SM双抗Hank’s液中剔除滑膜等外周组织，以眼科剪剪成1 mm3左右的碎片。配制0.25%的胶原酶Ⅱ，将韧带组织移至胶原酶液中，37℃条件下消化1～2 h，组织消化后，无血清DMEM培养液冲洗，离心，以含15%胎牛血清的DMEM培养液悬浮细胞，接种于一次性塑料培养瓶Corning公司，美国中，置于37℃、体积分数为5%CO2培养箱内培养。每3 d换液一次，待细胞汇合后，以胰蛋白酶消化传代。 1.3 XTT法测定韧带细胞增殖收获第2～5代的MCL和ACL细胞，以5×103孔的浓度，接种于96孔培养板上，待细胞贴壁后，分别加入1、5、10、50、100及500 ngml的bFGF和3.125、6.25、12.5、25、50及100 ngml的ECGF。对照组不加bFGF和ECGF。每组四个复孔。CO2培养箱内培养，每天观察一次。培养3 d后，每孔加入50 μl XTT1 mgml。在37℃、5%CO2培养箱内继续孵育，3 h后，在酶标仪上比色参考波长650 nm,测量波长450 nm，读取OD值。 1.4 统计学处理实验所得数据以SPSS11.0统计软件进行分析，用方差分析和Dunnett法检验，α=0.05。 2 结果 2.1 膝关节韧带细胞培养膝关节韧带细胞贴壁时间约5～6 d，体外培养条件下，ACL细胞的增殖速度明显慢于MCL细胞;ACL细胞原代生长约需13～15 d，MCL细胞原代生长约需11～13 d。1∶2传代的细胞不超过6代时，基本上能在5～7 d内汇合成单层。ACL与MCL细胞贴壁生长，形态呈梭形，与典型的成纤维细胞形态相似图1、2。毕业论文网图1 原代培养兔ACL细胞×100 图2 原代培养兔MCL细胞×100 2.2 bFGF对兔韧带成纤维细胞增殖的影响表1可见，MCL和ACL各实验组OD值均大于对照组，经统计学检验，ACL各组OD值方差分析，F=28.02，P各实验组与对照组比较，1 ngml组P0.05，≥5 ngml各组P均0.01。MCL各组OD值方差分析，F=90.66，P各实验组与对照组比较均为P0.01。表明bFGF对MCL和ACL细胞的增殖均有促进作用。1 ngml bFGF 即可促进MCL细胞生长，5 ngml bFGF 即可促进ACL细胞生长。bFGF浓度高至50 ngml时，其促进作用达到最大值;超过其最佳作用浓度后，随浓度升高促进作用不再增强P0.05。 2.3 ECGF对兔韧带成纤维细胞增殖的影响表2可见，MCL和ACL各实验组OD值均大于对照组，经统计学检验，ACL各组OD值方差分析，F=3.82，P=0.01;各实验组与对照组比较，≥12.5 ngml各组P值均0.05。MCL各组OD值方差分析，F=136.06，P各实验组与对照组比较均为P0.01。表明ECGF 对MCL和ACL细胞的增殖均有促进作用。3.125 ngml ECGF 即可显著促进MCL细胞生长，浓度在12.5 ngml时，其促进作用达到最大值;超过其最佳作用浓度后，随浓度升高促进作用不再增强P0.05。表1 bFGF对兔韧带细胞增殖的影响表2 ECGF对兔韧带细胞增殖的影响毕业论文网 3 讨论 bFGF和ECGF是一些具有生物活性的多肽，具有趋化性、促有丝分裂和促进胞外基质合成的作用。它们可与细胞表面特异性受体结合，产生第二信使;第二信使可使细胞核根据特定的基因诱导或抑制基因组DNA向mRNA的转录，改变某些蛋白质的水平，进而促进DNA的复制和有丝分裂。毕业论文网

碱性成纤维生长因子和人重组骨形态发生蛋白2诱导分化兔骨髓间充质干细胞

碱性成纤维生长因子和人重组骨形态发生蛋白2诱导分化兔骨髓间充质干细胞胡运生;范清宇;马保安;张殿忠;刘云燕【期刊名称】《中国组织工程研究》【年(卷),期】2006(010)001【摘要】背景:骨髓间充质干细胞依赖特定的生长和分化因子能分化为骨、软骨、脂肪等不同组织.人重组骨形态发生蛋白2有明显的骨诱导作用,能诱导未分化的骨髓间充质干细胞不可逆地形成骨与软骨,并最终导致新骨生成;碱性成纤维生长因子亦参与调控骨髓间充质干细胞的软骨与骨的分化.目的:观察碱性成纤维生长因子和人重组骨形态发生蛋白2对兔骨髓间充质干细胞增殖和分化的影响,以便寻求合适的骨形成诱导方法以代替常规的成骨培养体系.设计:随机对照实验.单位:解放军第四军医大学唐都医院骨肿瘤研究所.材料:培养的兔骨髓间充质干细胞.方法:实验于2004-06/12在第四军医大学唐都医院骨肿瘤研究所暨全军骨科中心完成. ①体外培养的兔骨髓间充质干细胞分别经3种不同的生长因子(100μg/L人重组骨形态发生蛋白2,100μg/L碱性成纤维生长因子,100μg/L人重组骨形态发生蛋白2加100μg/L碱性成纤维生长因子)和成骨培养体系处理. ②其增殖和分化通过四甲基偶氮唑盐比色法,碱性磷酸酶、骨钙素检测和钙结节Von Kossa染色观察.主要观察指标:通过检测增殖率和碱性磷酸酶活性比较不同生长因子对兔骨髓间充质干细胞增殖和分化的影响.结果: ①人重组骨形态发生蛋白2能促进兔骨髓间充质干细胞的增殖和分化,特别是其分化. ②碱性成纤维生长因子能刺激兔骨髓间充质干细胞的增殖,与对照组相比,细胞增值率提高近100%;虽然对兔骨髓问充质干细胞分化无明显影响,但其能促进人重组骨形态发生蛋白2对兔骨髓间充质干细胞分化.结论:碱性成纤维生长因子和人重组骨形态发生蛋白2是兔骨髓间充质干细胞有效的分化因子,两者在体外都能促进兔骨髓间充质干细胞的增殖和分化.碱性成纤维生长因子和人重组骨形态发生蛋白2能协同作用加速骨诱导及骨形成,可以用作分化骨组织工程中的种子细胞.%BACKGROUND: Mesenchymal stem cells (MSCs) are capable of differentiating into a variety of lineages, including bone, cartilage, or fat, depending on the inducing stimuli, specific growth and differentiation factors.Recombinant human bone morphogenetic protein 2 (rhBMP-2) produced by gene engineering has an obvious osteoinductive activity and can induce undifferentiated mesenchymal cells into cartilage and bone irreversibly, resulting in new bone formation. Basic fibroblast growth factor (bFGF) modulates chondrogenic and osteogenic differentiation of MSCs.OBJECTIVE: To investigate the effect of bFGF and rhBMP-2 on the differentiation and proliferation of cultured rabbit mesenchymal stem cell in order to find out an optimal way of osteogenesis instead of conventional osteogenic supplements (OS).DESIGN: A randomized and controlled trial.SETTING: General Institute of Orthopaedic Oncology, Tangdu Hospital,Fourth Military Medical University of ChinesePLA.MATERIALS: The subjects were rabbit mesenchymal stem cells cultured by the author.METHODS: This experiment was conducted at the Center of Orthopaedic Surgery, General Institute of Orthopaedic Oncology, Tangdu Hospital,Fourth Military Medical University of Chinese PLA from June 2004 to December 2004. ①Rabbit MSCs cultured in vitro were treated with different growth factor (100 μg/L rhBMP-2, 100 μg/L bFGF, 10 μg/L rhBMP-2and 100 μg/L bFGF and OS; ②The proliferation and differentiationof MSCs were observed through activity of MTT, expression of alkaline phosphatase(ALP), and von Kossa staining.MAIN OUTCOME MEASURES: the rate of proliferation and the activity of ALP.RESULTS: ①rhBMP-2 could promote the proliferation and differentiation of MSCs, especially the cell differentiation; ②bFGF could stimulate the proliferation , the cellular proliferation rate increased 100% as compared with control group, and has no effect on differentiation of MSCs , but it could enhance effect on the cell proliferation of rhBMP-2.CONCLUSION: bFGF and rhBMP-2 are effective induction factors for MSCs. Both of them can stimulate the proliferation and differentiation of MSCs in vitro. bFGF and rhBMP-2 exerted a synergetic action in speeding up the pace of osteoinduction and osteogenesis and can be used to differentiate seed cells for tissue engineering bone.【总页数】3页(P163-165)【作者】胡运生;范清宇;马保安;张殿忠;刘云燕【作者单位】解放军第四军医大学唐都医院骨肿瘤研究所暨全军骨科中心,陕西省西安市,710038;解放军第四军医大学唐都医院骨肿瘤研究所暨全军骨科中心,陕西省西安市,710038;解放军第四军医大学唐都医院骨肿瘤研究所暨全军骨科中心,陕西省西安市,710038;解放军第四军医大学唐都医院骨肿瘤研究所暨全军骨科中心,陕西省西安市,710038;解放军第四军医大学唐都医院骨肿瘤研究所暨全军骨科中心,陕西省西安市,710038【正文语种】中文【中图分类】R394.2【相关文献】1.人骨形态发生蛋白2基因重组腺病毒表达载体转染兔骨髓间充质干细胞 [J], 何春耒;姬广林;刘午阳;吴东宝;何澄;王茂源;叶勇军;莫建文;高辉2.人骨形态发生蛋白2基因重组腺病毒表达载体转染兔骨髓间充质干细胞 [J], 何春耒;姬广林;刘午阳;吴东宝;何澄;王茂源;叶勇军;莫建文;高辉3.重组人甲状旁腺素对人骨髓间充质干细胞表达骨形态发生蛋白2的影响 [J], 李丁;王俊芳;张成玉4.骨形态发生蛋白2及碱性成纤维生长因子2对大鼠骨髓间充质干细胞膜片增殖和成骨分化的影响 [J], 张文静; 王佳; 田梦婷; 韩祥祯; 周琦琪; 何惠宇5.骨形态发生蛋白2及碱性成纤维生长因子2对大鼠骨髓间充质干细胞膜片增殖和成骨分化的影响 [J], 张文静; 王佳; 田梦婷; 韩祥祯; 周琦琪; 何惠宇因版权原因，仅展示原文概要，查看原文内容请购买。

兔骨髓间充质干细胞的生物学特征及其对不同生长因子的反应

兔骨髓间充质干细胞的生物学特征及其对不同生长因子的反应【摘要】为了研究兔骨髓间充质干细胞(rBM-MSC)的生物学特征及其对不同生长因子的反应，使用贴壁培养法从兔骨髓单个核细胞中获得间充质干细胞，在光学显微镜和电子显微镜下观察其基本生长行为和生物学特征；使用流式细胞仪检测rBM-MSC的免疫学表型；RT-PCR法检测其胶原表达；诱导rBM-MSC多向分化并使用特异性染色和RT-PCR予以鉴定；最后使用MTT法检测IL-1、3、8和HGF对rBM-MSC生长增殖的影响。

结果显示：贴壁生长的rBM-MSC具有典型的成纤维样细胞形态，可传15代以上，第5代rBM-MSC高表达基质受体CD44，低表达造血细胞标记CD45；RT-PCR显示高表达I型胶原，弱表达II型胶原，不表达X型胶原；在不同的诱导条件下，rBM-MSC 可被诱导分化为成骨细胞、软骨细胞、脂肪细胞和神经元样细胞。

rBM-MSC对IL-3最为敏感，10 ng/ml的低浓度IL-3可显着促进细胞增殖达32％以上，而高浓度IL-3能显着抑制其生长。

结论：分离培养了rBM-MSC，其生物学特征与人和猕猴等BM-MSC相似的生物学特性，低浓度IL-3可有效促进其增殖。

【关键词】骨髓；间充质干细胞；生长因子；兔Biological Characteristics of Rabbit Bone Marrow Mesenchymal Stem Cells and Their Response to Different Growth FactorsAbstractThis study was aimed to analyze the biological characteristics of rabbit bone marrow mesenchymal stem cells (rBM-MSCs) and their response to different growth factors. RabbitBM-MSCs were separated from bone marrow mononuclear cells by using adherent cultivation. Biologicalcharacteristics were investigated by optical and electron microscopy. Immunophenotype of rBM-MSCs was measured by flow cytometry. Theexpression of collagen was detected by RT-PCR. Differentiation potential was identified by specific staining andRT-PCR. The response of rBM-MSCs to IL-1, 3, 8 and HGF with different concentrations were tested by MTT. The results showed that the rBM-MSCs gave rise to a population of adherent cells characterized by the presence of a predominant cell type with a typical fibroblast-like morphology and could be cultured for over 15 passages. CD44 was highly expressed on F5 rBM-MSCs (32%) and CD45 was lowly expressed (%). Type I collagen was highly expressed, while type II collagen was lowly expressed and type X collagen was not detected on rBM-MSCs using RT-PCR method. In various conditions inducting differentiation, rBM-MSCs could differentiate into the osteoblast, chondrocyte, adipocyte and neuron-like cells. The rBM-MSCs weresensitive to IL-3, even low concentration (10 ng/ml) of IL-3 could promote the proliferation of rBM-MSCs effectively (32％, ), whereas high concentration IL-3 inhibited it significantly. It is concluded that rabbit BM-MSCs were successfully isolated and culture-expanded. The biological characteristics of rabbit BM-MSCs are similar to those of human and rhesus BM-MSCs. IL-3 with low concentration can promote the proliferation of rBM-MSCs effectively, but high concentration of IL-3 can inhibit their proliferation.Key wordsbone marrow; mesenchymal stem cell; growth factor; rabbit骨髓间充质干细胞具有高度自我更新和多向分化的潜能，在不同的诱导条件下，可分化为多种组织细胞类型，如骨髓基质细胞、成骨细胞、成软骨细胞和脂肪细胞［1］。

碱性成纤维细胞生长因子对小鼠骨髓内皮细胞增殖的促进作用

碱性成纤维细胞生长因子对小鼠骨髓内皮细胞增殖的促进作用王峰;张煜;李娜娜【期刊名称】《新乡医学院学报》【年(卷),期】2006(023)005【摘要】目的以内皮细胞培养基(Endo-M)培养小鼠骨髓内皮细胞,观察碱性成纤维细胞生长因子(bFGF)对骨髓内皮细胞增殖的影响.方法用Endo-M培养小鼠骨髓内皮细胞,通过Wright's Giemsa染色计数内皮细胞集落,免疫荧光法观察内皮细胞表型;加入不同浓度的bFGF,通过集落形成率、MTT法和流式细胞仪检测内皮细胞生长周期,观察bFGF对内皮细胞体外扩增的影响.结果 Endo-M 诱导的骨髓细胞可以生成内皮细胞,vWF检测阳性.加入不同浓度的bFGF,集落计数及MTT法均证实了bFGF对内皮细胞的增殖具有明显的促进作用,比较实验组(bFGF组)和对照组细胞的生长曲线,两者有明显差异(P＜0.05).结论 bFGF对内皮细胞的增殖具有促进作用.【总页数】3页(P475-477)【作者】王峰;张煜;李娜娜【作者单位】新乡医学院生理学与神经生物学教研室,河南,新乡,453003;新乡医学院生物化学与分子生物学教研室,河南,新乡,453003;新乡医学院人体解剖学教研室,河南,新乡,453003【正文语种】中文【中图分类】Q462;R331.2【相关文献】1.促进小鼠骨髓间充质干细胞增殖和成骨分化的血管内皮生长因子 [J], 张连方;康辉;邓廉夫;杨惠林2.小鼠骨髓间充质干细胞对人舌鳞状细胞癌Cal27细胞的归巢作用及其对Cal27细胞增殖和迁移的促进作用 [J], 孟琳;王璐;布文奂;丁鑫鑫;高华丽;王梓霖;刘玉兰;李琛;孙宏晨3.体外负压培养对小鼠骨髓间充质干细胞增殖能力和血管内皮生长因子分泌水平的影响 [J], 杨雄峰; 姜慧娇; 王小义; 郭黎姣; 周青; 韩欢欢; 李林林; 廖振宇; 雷振; 陈雪玲; 张宏伟; 吴向未4.体外负压培养对小鼠骨髓间充质干细胞增殖能力和血管内皮生长因子分泌水平的影响 [J], 杨雄峰; 陈雪玲; 张宏伟; 吴向未; 姜慧娇; 王小义; 郭黎姣; 周青; 韩欢欢; 李林林; 廖振宇; 雷振5.骨髓内皮细胞无血清条件培养液对骨髓内皮细胞增殖的促进作用 [J], 周小莹;王绮如;黄艳红;程腊梅;谭孟群因版权原因，仅展示原文概要，查看原文内容请购买。

基因重组腺病毒载体与慢病毒载体转染兔骨髓间充质干细胞的对比

基因重组腺病毒载体与慢病毒载体转染兔骨髓间充质干细胞的对比李诗鹏;李强;石正松;蔡伟良;宁寅宽;陶旋【摘要】背景:腺病毒载体和慢病毒载体均是较好的组织工程基因载体,两者在介导骨形态发生蛋白2转染兔骨髓间充质干细胞中的差异尚待探究.目的:比较腺病毒和慢病毒载体介导EGFP/骨形态发生蛋白2转染体外培养兔骨髓间充质干细胞的转导效率、持续时间及外源基因表达差异.方法:将第5代兔骨髓间充质干细胞分3组培养,A组以腺病毒载体介导EGFP/骨形态发生蛋白2(Ad-EGFP/BMP-2)转染细胞,B组以慢病毒载体介导EGFP/骨形态发生蛋白2(Lenti-EGFP/BMP-2)转染细胞,C组为未进行转染.转染24 h、48 h、72 h、1周、3周,检测EGFP基因表达;转染72 h后,免疫组织化学观察细胞骨形态发生蛋白2的表达;转染后72 h、1周、3周,Western blot检测骨形态发生蛋白2蛋白表达.结果与结论:①转染24 h后,A、B组可见EGFP表达,A组明显强于B组;转染48 h后,A、B组荧光进一步增强;转染72 h后,A组荧光强度近高峰,B组荧光持续增强.转染1周后,A组荧光强度开始下降,B组荧光强度仍然增强.转染3周后,A组荧光强度明显下降甚至消失,B组荧光强度有所下降,但仍然保持一定的表达.C组在各时间点均无EGFP表达;②A、B组胞浆均呈骨形态发生蛋白2阳性表达,C组呈阴性表达;③转染72 h后,A组骨形态发生蛋白2蛋白表达量强于B组;转染1周后,A组表达下降,B组表达增强并强于A 组;转染3周后,A组表达微弱,B组持续表达且明显强于A组;④结果表明,相对腺病毒载体,慢病毒载体介导EGFP/骨形态发生蛋白2基因转染兔骨髓间充质干细胞在表达持续时间上具有一定的优势.%BACKGROUND: Both lentiviral vector and adenoviral vector are considered as good vectors for gene mediation, and their differences in transferring bone morphogenetic protein 2 (BMP-2)into rabbit bone marrow mesenchymal stem cells (BMSCs) are unclear. OBJECTIVE: To compare the duration, efficiency and the deviation of exogenous gene expression after rabbit BMSCs transfection using lentiviral vector and adenoviral vector which are used to mediate enhanced green fluorescent proteins (EGFP) and BMP-2. METHODS: Rabbit BMSCs at passage 5 were exposed to Ad-EGFP-BMP-2 (group A) or Lenti-EGFP-BMP-2 (group B) with multiplicity of infection of 100, as transfection groups. And in control group (group C), the same quality of culture medium was required equivalent to the groups A and B. The expression of EGFP was observed by inverted fluorescence microscope at various time intervals. And the expression of exogenous gene BMP 2 in cells was detected and analyzed by immunohistochemical staining at 72 hours after transfection as well as by western blot at 72 hours, 1, 3 weeks after transfection. RESULTS AND CONCLUSION: The intense green fluorescence emerged under the microscope at 24-48 hours after transfection in group A, which was stronger than group B, reached the peak at 72 hours, and then decreased at 1 week until disappearance at 3 weeks. No EGFP expression was detected in group C. High expression of BMP-2 was found in group A but was dramatically downregulated after 1 week. Group B showed the high expression of EGFP/BMP-2 persisted for a longer period after transfected that even lasted for 3 weeks. Overall, the lentiviral vector and adenoviral vector can efficiently transfect rabbit BMSCs and stably express the target gene of EGFP/BMP-2. Under the same MOI, compared to the adenoviral vector, transfection of lentiviral vector to rabbit BMSCs is moreeffectively and expression of EGFP/BMP-2 can be persistent in a longer term.【期刊名称】《中国组织工程研究》【年(卷),期】2017(021)009【总页数】6页(P1340-1345)【关键词】干细胞;骨髓干细胞;慢病毒载体;腺病毒载体;骨髓间充质干细胞;基因转染;骨形态发生蛋白类;国家自然科学基金【作者】李诗鹏;李强;石正松;蔡伟良;宁寅宽;陶旋【作者单位】桂林医学院附属医院急诊创伤外科,广西壮族自治区桂林市 541001;桂林医学院附属医院急诊创伤外科,广西壮族自治区桂林市 541001;桂林医学院附属医院急诊创伤外科,广西壮族自治区桂林市 541001;桂林医学院附属医院急诊创伤外科,广西壮族自治区桂林市 541001;桂林医学院附属医院急诊创伤外科,广西壮族自治区桂林市 541001;桂林医学院附属医院急诊创伤外科,广西壮族自治区桂林市 541001【正文语种】中文【中图分类】R394.20 引言 Introduction骨组织工程在治疗骨科疾病如骨缺损等方面展现了良好的前景[1]。

《生长分化因子-5及碱性成纤维细胞生长因子诱导家兔椎间盘髓核细胞成骨的研究》

《生长分化因子-5及碱性成纤维细胞生长因子诱导家兔椎间盘髓核细胞成骨的研究》摘要：本研究旨在探讨生长分化因子-5（GDF-5）及碱性成纤维细胞生长因子（bFGF）对家兔椎间盘髓核细胞（Nucleus Pulposus，NP）成骨的诱导作用。

通过体外细胞培养和体内动物实验，观察两种生长因子对家兔椎间盘髓核细胞向成骨细胞分化的影响，以期为椎间盘退行性疾病的治疗提供新的思路和方法。

一、引言椎间盘退行性疾病是骨科领域的常见病，其发病机制与椎间盘髓核细胞的生长分化密切相关。

近年来，生长因子在促进细胞增殖、分化及组织修复中的作用日益受到关注。

其中，GDF-5和bFGF在骨形成和软骨修复方面表现出显著效果。

因此，研究这两种生长因子对家兔椎间盘髓核细胞成骨的诱导作用，对于理解椎间盘退行性疾病的发病机制及寻找新的治疗方法具有重要意义。

二、材料与方法1. 材料准备选取健康成年家兔，获取其椎间盘髓核组织，进行细胞培养和生长因子的准备。

同时，准备相应的实验试剂和设备。

2. 细胞培养与分组将家兔椎间盘髓核细胞进行体外培养，并分为实验组和对照组。

实验组分别加入不同浓度的GDF-5和bFGF，对照组则加入等量的培养基。

3. 实验方法通过实时定量PCR、免疫荧光染色、Western Blot等技术手段，观察两种生长因子对家兔椎间盘髓核细胞成骨相关基因和蛋白表达的影响。

同时，进行体内动物实验，观察两种生长因子对家兔椎间盘退行性疾病的治疗效果。

三、结果1. 细胞实验结果细胞实验结果显示，GDF-5和bFGF能够显著促进家兔椎间盘髓核细胞成骨相关基因和蛋白的表达。

在实验组中，随着生长因子浓度的增加，成骨相关基因和蛋白的表达水平逐渐升高。

2. 动物实验结果动物实验结果显示，GDF-5和bFGF能够有效地改善家兔椎间盘退行性疾病的症状。

治疗后，家兔的疼痛程度降低，活动能力提高，影像学检查显示椎间盘结构得到改善。

四、讨论本研究结果表明，GDF-5和bFGF能够诱导家兔椎间盘髓核细胞向成骨细胞分化，从而促进骨的形成和椎间盘的修复。

碱性成纤维细胞生长因子基因转染骨髓干细胞与韧带成纤维细胞共培养效应

４３００７１中图分类号：Ｒ３９４２文献标识码Ａ
文章编号：２０９５４３４４
于细咆：骨髓干细跑：碱性戒纤维细验生长因子：豫病毒：转染：韧带成纤维细咆：国家自然科学基金
主题词：
骨髓：阃硬干细胞：髌韧带；成纤维细胞基金资助：国家自然辩学基金项巨（Ｂ１２０１４０１、
ｈｔｔｐ：／／ＶＷＶＷｃｒｔｅｒ．ｏｒｇ
摘要
背景：碱性成纤维细胞生长因子在韧带损伤愈合中具有重要作用，细胞因子转染的骨髓间充质干细胞可作为种子细胞应用于韧带组织工程。
目的：探讨人碱性成纤维细胞生长因子基因修饰的骨髓间充质干细胞与韧带成纤维细胞三维共培养后的交互
维细胞分化，均有利于促进韧带损伤的内源性愈合。
关键词：
李斌，男，１９９０年生，湖北省钟祥市人，汉族，在读硕士，主要从事关节外科、骨组织工程研究。
通讯作者：陈彪，博士，主治医师，武汉大学中南医院骨科，湖北省武汉市
化的能力。
李斌陈廖斌，齐勇建，葫东才．陈彪碱牲或纤维细跑生长因子基函转染骨髓干细咆ｓ韧带或纡维细瞻共培养效应【Ｊ】．中国组织Ｉ程研究．２０１５．１９（２Ｂ）：４４２９－４４３４．ｄｏｉ：ｌ０．３９６９／ｊ．ｉｓｓｎ．２０９５－４３４４．２０１５．２８．００１

碱性成纤维细胞生长因子对兔骨髓基质细胞生物行为的影响

碱性成纤维细胞生长因子对兔骨髓基质细胞生物行为的影响刘建;常祺;胡蕴玉;孟国林【期刊名称】《中国组织工程研究》【年(卷),期】2003(007)023【摘要】目的:观察兔骨髓基质细胞 (MSC)经不同剂量碱性成纤维细胞生长因子( bFGF)刺激后,促血管内皮细胞增生的重要递质血管内皮细胞生长因子( VEGF)在MSC中的表达及分布情况,并与相应成骨情况做一对比,借以预测 MSC经 bFGF刺激后成骨效能与成血管效能的变化,探讨其对剂效关系. 方法:取兔双侧股骨 MSC,采用 MSC体外培养技术,分别以不同剂量 bFGF刺激细胞,并设立空白对照组.培养 5 d后,进行细胞形态( HE染色)、增殖情况(细胞记数法与 MTT法)、碱性磷酸酶(改良钙钴法染色)、钙化结节(图像分析)、 VEGF阳性细胞率(免疫组化染色)等项目的检测. 结果:不同剂量组间在细胞记数法、 MTT法检测、碱性磷酸酶活性、矿化面积百分率、 VEGF阳性细胞率 5项观测指标上 F值分别为 65.50, 22.47, 11.12, 8.33和 224.37, P< 0.01,差别具有显著意义,结合各组均值来看, bFGF剂量为 1 200 U/mL左右时效果最佳. 结论:应用 bFGF在促进 MSC增殖的同时,还可促进促血管内皮细胞增生的重要递质- VEGF的表达,其应用剂量与效果之间存在明显相关关系. bFGF应用剂量为 1 200 U/mL左右时,其促进 MSC表达 VEGF及促进细胞增殖作用同时达到最佳效果,超过此应用剂量,两者则有下降趋势.【总页数】3页(P3184-3186)【作者】刘建;常祺;胡蕴玉;孟国林【作者单位】解放军第四军医大学西京医院骨科研究所,陕西省西安市,710032;解放军第四军医大学西京医院骨科研究所,陕西省西安市,710032;解放军第四军医大学西京医院骨科研究所,陕西省西安市,710032;解放军第四军医大学西京医院骨科研究所,陕西省西安市,710032【正文语种】中文【中图分类】R318【相关文献】1.骨形态发生蛋白微球对兔骨髓基质细胞生物学行为的影响 [J], 覃昱;裴国献;谢德明;金丹;魏宽海2.bFGF对兔骨髓基质细胞VEGF因子表达及细胞生物行为的影响 [J], 常祺;刘建;胡蕴玉;孟国林;白建萍;彭磊3.骨形态发生蛋白-2对兔骨髓基质细胞生物行为的影响 [J], 刘建;常祺;胡蕴玉;孟国林;白俊4.复合骨形态发生蛋白2及碱性成纤维细胞生长因子注射型骨修复材料对兔骨髓基质细胞增殖和超微结构的影响 [J], 崔赓;胡蕴玉;雷伟;孙明林;李洁;靳小兵;汪培铭;杨柳;吕昌伟;吕荣5.碱性成纤维细胞生长因子对体外培养的兔骨髓基质细胞生长特性的影响 [J], 王志勇;陈增海;于胜吉因版权原因，仅展示原文概要，查看原文内容请购买。

基因重组人碱性成纤维细胞生长因子药物动力学实验研究 ——家兔静脉快推给药室分析

基因重组人碱性成纤维细胞生长因子药物动力学实验研究——家兔静脉快推给药室分析张萱;张铭;苏旭;刘建香;李凤兰;马晓辉【期刊名称】《吉林大学学报（医学版）》【年(卷),期】1999(025)003【摘要】目的:研究家兔静脉快推基因重组人碱性成纤维细胞生长因子(rhbFGF)后血药浓度随时间的变化关系,并对其代谢动力学参数进行估计.方法:家兔静脉一次快推rhbFGF 4000 IU(10μg),给药后10,20,30min及1.0,1.5,2.0,3.0,4.0,6.0,8.0 h 耳缘静脉采血,放免分析法检测血中各个时间点的药物浓度.用数学方法拟合血药浓度一时间的函数关系,并估计药物动力学参数.结果:家兔静脉一次快推给药后血中rhbFGF浓度属双室模型一级动力学特征,其血药浓度一时间函数的解析表达式为:G=90.1156e-2.0107t+13.0707e-0.1783t.结论:本研究为指导rhbFGF的临床应用提供药物动力学的基本依据.【总页数】3页(P226-228)【作者】张萱;张铭;苏旭;刘建香;李凤兰;马晓辉【作者单位】卫生部放射生物学重点实验室,长春,130021;卫生部放射生物学重点实验室,长春,130021;卫生部放射生物学重点实验室,长春,130021;卫生部放射生物学重点实验室,长春,130021;卫生部放射生物学重点实验室,长春,130021;卫生部放射生物学重点实验室,长春,130021【正文语种】中文【中图分类】R965【相关文献】1.Excel联合Visual Basic辅助制定一室模型静脉推注给药方案设计 [J], 陈领弟;苏银法2.微分法计算二室模型药物静脉推注的药物动力学参数 [J], 苏银法;林中3.药物动力学双室模型静脉注射给药模拟实验设计 [J], 何晓明;杨少坤;党云洁;王伟;杜青4.基于EXCEL软件的二室模型静脉推注给药的给药方案设计 [J], 苏银法5.抗生素在椎间盘髓核组织内药物动力学变化的实验研究──静脉和经皮穿刺椎间盘给药的比较 [J], 谢学斌;杨建勇;余文昌;庄文权;邹学农因版权原因，仅展示原文概要，查看原文内容请购买。

碱性成纤维细胞生长因子基因修饰骨髓间充质干细胞移植修复急性肾损伤

碱性成纤维细胞生长因子基因修饰骨髓间充质干细胞移植修复急性肾损伤崔利德;李铁民【期刊名称】《中国组织工程研究》【年(卷),期】2016(020)028【摘要】BACKGROUND:It has been confirmed that basic fibroblast growth factor (bFGF) can promote the growth, proliferation, differentiation and functional expression of most cels derived from neuroderm and mesoderm. OBJECTIVE:To investigate the effect of bFGF-transfected bone marrow mesenchymal stem cel transplantation in rats with acute kidney injury. METHODS: bFGF genes were transfected into bone marrow mesenchymal stem cels via an adenovirus vector, and then expression of bFGF in transfected cels was identified using RT-PCR technology. Rat models of acute kidney injury were prepared by clipping bilateral renal pedicles, and then randomized into three groups (n=20): rats were given injection of bone marrow mesenchymal stem cel suspensionsvia tail vein as negative transfected group, those given injection of bFGF-transfected bone marrow mesenchymal stem cel suspensionsvia tail vein as bFGF-transfected group, and the others given injection of DMEMvia tail vein as model group. Four weeks later, levels of serum creatinine and urea nitrogen were detected, expressions of connective tissue growth factor and growth factor in renal tissues were detected by Western blot assay, and morphology of renaltissues was observed using hematoxylin-eosin staining. RESULTS AND CONCLUSION:bFGF genes were successfuly transfected into bone marrow mesenchymal stem cels. Compared with the model group, the levels of serum creatinine and urea nitrogen were significantly reduced in bFGF-transfected and negative transfected groups, especialy in the bFGF-transfected group (P < 0.05), while expressions of connective tissue growth factor and transforming growth factor in renal tissues in bFGF-transfected and negative transfected groups were significantly weakened in these two groups (P < 0.05), but there were no significant differences between the bFGF-transfected group and negative transfected group (P > 0.05). Besides, renal tissues necrosis and inflammatory reactions were mitigated in the negative transfected group; renal tubules with normal outlines and no overt necrotic cels could be found in the bFGF-transfected group. These findings show that bFGF-transfected bone marrow mesenchymal stem cel transplantation plays a better role in acute kidney injury repair in rats.%背景：研究表明，碱性成纤维细胞生长因子对源于中胚层和神经外胚层的大部分细胞均有促进生长、增殖、分化及功能表达的作用。

碱性成纤维细胞因子对兔股骨头坏死模型实验修复过程的影响

碱性成纤维细胞因子对兔股骨头坏死模型实验修复过程的影响周华江;聂林;张琦;薛强;李金花【期刊名称】《中国矫形外科杂志》【年(卷),期】2002(9)9【摘要】目的 :探讨碱性成纤维细胞因子对股骨头坏死有无促进修复作用。

方法 :健康兔用液氮冷冻法造成左侧股骨头坏死 ,在同侧股骨颈中央钻直径 1.5mm孔 ,实验组术中及术后第 2、4、6d局部注射碱性成纤维细胞因子 (basicfi broblastgrowthfactor ,bFGF) 2 0 0UI/次 ,术后 2、4、8周分别处死各 4只动物 ,观察有关指标。

结果 :(1)X线检查结合计算机相对骨密度分析 :2周时两组股骨头骨密度均未见增高 ,4、8周时均增高 ,但手术侧实验组股骨头骨密度显著高于对照组 (P <0 .0 5 )。

(2 )单光子骨密度 (BMD)测定 :4周以后手术侧实验组股骨头骨密度显著高于对照组 (P <0 .0 5 )。

(3 )组织学观察 :4周时实验组手术侧股骨头内纤维组织增生和成骨细胞增生明显 ,新骨形成多于对照组 ,8周时实验组手术侧股骨头内坏死修复大部分已完成 ,而对照组坏死修复仍在大部分区域内进行。

结论 :碱性成纤维细胞因子对液氮冷冻兔股骨头坏死模型实验修复过程有促进作用。

【总页数】3页(P895-897)【关键词】碱性成纤维细胞因子;兔;股骨头坏死;病理【作者】周华江;聂林;张琦;薛强;李金花【作者单位】济南市第四人民医院骨科;山东大学齐鲁医院骨科;济南军区总医院骨矿研究室【正文语种】中文【中图分类】R681.802【相关文献】1.碱性成纤维细胞因子和骨形态发生蛋白对股骨头坏死修复过程的研究 [J], 靳松;沈靖南;王晋;尹军强;邹昌业;郭乾臣2.被动应力训练对兔冈上肌腱急性断裂术后修复过程中碱性成纤维细胞生长因子表达的影响 [J], 李森;闵少雄;张辉;付国建;王鹏程;靳安民3.左旋多巴对兔股骨头坏死模型实验修复过程的影响 [J], 吕智;刘小丽;董天华4.双相陶瓷生物骨-骨形态发生蛋白-碱性成纤维细胞生长因子复合物修复股骨头坏死模型的实验研究 [J], 王蕾; 彭吾训; 张爱华; 邓进; 龚跃昆; 李世和; 胡蕴玉5.双相陶瓷生物骨-骨形态发生蛋白-碱性成纤维细胞生长因子复合物修复股骨头坏死模型的实验研究① [J], 王蕾; 彭吾训; 张爱华; 邓进; 龚跃昆; 李世和; 胡蕴玉因版权原因，仅展示原文概要，查看原文内容请购买。

表皮生长因子与碱性成纤维生长因子对人牙髓干细胞增殖的影响

表皮生长因子与碱性成纤维生长因子对人牙髓干细胞增殖的影响徐皑;吴桂堂;刘兴容【期刊名称】《泸州医学院学报》【年(卷),期】2013(36)4【摘要】目的：探讨表皮生长因子(epidermal growth factor，EGF)和碱性成纤维生长因子（basic fibroblast growth factor， bFGF）对人牙髓干细胞(human dental pulp stem cells， hDPSCs)增殖能力的影响。

方法：采用噻唑蓝（MTT）比色法分别测定不同浓度EGF和bFGF在不同时间，单独或联合应用对hDPSCs 增殖能力的影响。

结果：50 ng/ml EGF和20 ng/ml bFGF促hDPSCs增殖能力最强。

以50ng/ml EGF、20ng/ml bFGF单独或联合作用，其促hDPSCs增殖能力均具有时间依赖性，第3 d、5 d、7 d， EGF、bFGF促hDPSCs增殖作用显著增强，二种生长因子联合作用，促增殖作用显著高于其单独作用效果，差异具有统计学意义（P<0.05）。

结论：生长因子EGF和bFGF最大效应浓度分别为：50 ng/ml、20 ng/ml。

EGF和bFGF单独作用均能显著促进hDPSCs的增殖，二者联合作用对hDPSCs的增殖有一定的协同作用。

%Objective: To investigate the effects of epidermal growth factor (EGF) and basic fibroblast growth factor(bFGF)on the proliferation of human dental pulp stem cells(hDPSCs) in vitro. Methods: Colorimetric assay was used to measure the dose - and time - depedent effects of EGF and bFGF on the proliferation of hDPSCs. Results:50 ng/ml EGF and 20 ng/ml bFGF had the strongeste effect on promoting the proliferation of hDPSCs re-spectively. The effect of 50ng/ml EGF and 20 ng/ml bFGF, whether alone or combined was time-depedent. It was significantly stronger at 3, 5, 7 d (P < 0.05), and the combined usege of the two cytokines was obviously stronger than used alone(P<0.05).Conclusion: The optimal concentration of EGF and bFGF is 50 ng/ml and 20 ng/ml respectively. Both EGF and bFGF can significantly promote the proliferation of hDPSCs. The combination use of two growth factors has a certain synergism.【总页数】4页(P356-359)【作者】徐皑;吴桂堂;刘兴容【作者单位】泸州医学院附属口腔医院口腔内科,四川泸州,646000;江苏省徐州市口腔医院牙体牙髓科;泸州医学院附属口腔医院口腔内科,四川泸州,646000【正文语种】中文【中图分类】R329.2【相关文献】1.表皮生长因子与碱性成纤维细胞生长因子对人牙髓干细胞分化的影响 [J], 吴桂堂;徐皑;刘兴容2.腺病毒介导表皮生长因子体外转染人牙髓干细胞对其凋亡的影响 [J], 徐皑;付广丽;刘兴容;鲍艳3.重组人表皮生长因子和碱性成纤维生长因子促进人角膜上皮细胞的增殖 [J], 彭艳阳;吴伟;曾丽娜;陈晓红;陆晓和;4.重组人表皮生长因子和碱性成纤维生长因子促进人角膜上皮细胞的增殖 [J], 彭艳阳;吴伟;曾丽娜;陈晓红;陆晓和5.聚对苯二甲酸乙二酯三维支架及碱性成纤维生长因子和乳酸对成人骨髓间充质干细胞增殖的影响 [J], 曹延粉;李丁;王菊芳因版权原因，仅展示原文概要，查看原文内容请购买。

碱性成纤维细胞生长因子基因转染对虹膜色素上皮细胞增殖及细胞周期的影响

碱性成纤维细胞生长因子基因转染对虹膜色素上皮细胞增殖及细胞周期的影响杨晓慧;孙葆忱【期刊名称】《郑州大学学报（医学版）》【年(卷),期】2008(043)005【摘要】目的:了解重组腺相关病毒(rAAV)载体介导碱性成纤维细胞生长因子(bFGF)基因转染对体外培养的虹膜色素上皮细胞(IPE)增殖及细胞周期的影响.方法:在体外培养的IPE细胞中加入转染rAAV-bFGF基因细胞和未转染细胞的培养液上清,继续培养4 h或1 d后分别应用MTT法和流式细胞仪检测IPE细胞的生长状况.结果:MTT法结果显示,加入rAAV-bFGF基因转染组培养液上清的IPE细胞D值为0.338±0.047,对照组为0.277±0.064,2组相比,t=3.745,P<0.05.流式细胞仪检测结果显示:加入rAAV-bFGF基因转染组培养液上清的S期IPE细胞为(21.19±1.93)%,与对照组(16.47±2.37)%相比.t=-3.448,P<0.05.结论:转染rAAV-bFGF的IPE细胞分泌的bFGF具有生物活性,这为rAAV-bFGF转染IPE细胞移植治疗视网膜色素变性提供了理论支持.【总页数】3页(P978-980)【作者】杨晓慧;孙葆忱【作者单位】郑州大学第一附属医院眼科,郑州,450052;首都医科大学附属北京同仁医院北京市眼科研究所,北京,100005【正文语种】中文【中图分类】R773.1【相关文献】1.基因转染的虹膜色素上皮细胞移植后RCS鼠视网膜BDNF表达观察 [J], 张英瑜;高朋芬;杨丽霞2.杆状病毒介导LacZ基因转染体外培养的人虹膜色素上皮细胞 [J], 彭颖;周翔天;付小莹;吕建新;瞿佳3.腺相关病毒介导bFGF基因对虹膜色素上皮细胞的转染及表达 [J], 杨晓慧;孙葆忱4.腺相关病毒介导色素上皮衍生因子转染人虹膜色素上皮细胞的体外研究 [J], 赵莼;王方;计菁;施沃栋;范先群;罗敏5.碱性成纤维细胞生长因子转染对半月板纤维软骨细胞增殖、细胞周期及胶原合成影响的研究 [J], 张经纬;曾炳芳;赵金忠因版权原因，仅展示原文概要，查看原文内容请购买。