茄子枯萎病菌致病效应因子的预测分析

萎病菌Fomgl4004全基因组16485个蛋白质序列中，经SignalP v4. 1分析后得到1601个具有信号肽的蛋白序列；然后经过TMHMM v2.0分析得到1481个跨膜数小于2的舊白序列;1481个具有信号肽的舊白经过TargetP vl. 1分析得到1449个定位为S的
舊白序列；最后经过ProtComp v9.0分析，得到1376个舊白质定位于细胞质中的分泌蛋白序列。1376个蛋白中，富含cysteine(大 于或等于6)的蛋白序列共733个，其中219个蛋白序列的multiple tandem mpeats大于或等于9。将219个蛋白序列和PHI数据库 进行比对筛选，并挑取小于300个氨基酸的蛋白序列，共筛选到29个致病相关候选效应因子。【结论】本研究最终从16485个蛋
2019年32卷6期 Vol. 32 No. 6
西希叙业学掖 Southwest China Journal of Agricultural Sciences
1285
文章编号：1001 -4829(2019)6ቤተ መጻሕፍቲ ባይዱ1285 -05
DOI: 10.16213/j. cnki. scjas. 2019. 6. 013
茄子枯萎病菌致病效应因子的预测分析
董章勇，陈欣瑜，舒永馨，罗 梅，向梅梅*
(仲恺农业工程学院,广东广州510225)
摘要:【目的】本研究为了获得由尖抱镰刀菌引起的茄子枯萎病菌的致病相关效应蛋白。【方法】通过生物信息学的方法进行预
测分析，包括SignalP、TMHMM、TargetP、ProtComp、与Pathogen-Host Interaction(PHI)数据库进行比对分析等方法。【结果】从茄子枯
DONG Zhang-yong, CHEN Xin-yu, SHU Yong-xin, LUO Mei, XIANG Mei-mei* (Zhongkai University of Agriculture and Engineering, Guangdong Guangzhou 510225, China)
白序列中筛选得到29个候选致病效应因子。为进一步研究该病菌的效应因子致病功能提供基础，也为其他病原菌效应因子的预 测分析提供方法参考。
关键词:茄子枯萎病菌;尖抱镰刀菌;效应因子;全基因组;分析
中图分类号：S641.1
文献标识码:A
Prediction and Analysis of Pathogenic Effector of Fusarium oxysporum f. sp. melongenae
Abstract: [ Objective] This study screened and predicted the effector proteins of Fusarium, oxysporum f. sp. melongenae. [ Method] Predictive analysis was carried out by bioinformatics methods, including SignalP, TMHMM, TargetP, ProtComp, and comparison with Pathogen-Host Interaction Database (PHI). [ Result] On the basis of the 16 485 protein sequences of the whole genome of eggplant Fusarium wilt Fomgl4004, 1601 protein sequences with signal peptides were obtained by SignalP v4. 1 analysis, and then 1481 protein sequences, which were smaller than 2, were obtained by TMHMM v 2.0 analysis, and 1481 had signal peptides. The protein sequence was analyzed by Tar­ getP vl. 1 analysis by TargetP vl. 1 analysis. Finally, the secretory protein sequence of the cytoplasm was obtained by ProtComp v9.0 analy­ sis. The 1376 proteins contained 733 protein sequences of cysteine content greater than or equal to 6, of which 219 protein sequences multi­ ple tandem repeats were greater than or equal to 9. 219 protein sequences and Pathogen-Host Interaction database ( PHI) database were com­ pared and screened and the protein sequences of less than 300 amino acids were selected, and 29 candidate effect factors were screened. [Conclusion] A total of 29 proteins were selected as putative effectors from Fomgl4004. Our study will provide useful information for manag­ ing Fusarium wilt of eggplant, and insights into the reference for the prediction of other phytopathogens. Key words: Fusarium wilt ； Fusarium oxysporum f. sp. melongenae ； Effectors ； Genome ； Analysis