分子细胞遗传学课程报告_PPT幻灯片
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marking chromatin mass edges),
rapid, short-distance movements of small chromosome segments (blue and green), slower-paced movements of chromosome segments inside the chromatin mass (red). 2、 Cumulative tracks marking rotational movements of the entire chromatin in the two meiocytes traced in A after 145 s. Starting and ending positions of the rotating chromatin in the nuclei from B and D. 3、 Cumulative tracks of the small chromosome segments tracked in the two meiocytes in A after 145 s
4 、Chromosome motility was coincident with dynamic deformations of the nuclear envelope
5 、 Both, chromosome and nuclear envelope motility depended on actin microfilaments as well as tubulin
❖ Chromosome dynamics of prophase I have been studied in budding and fission yeasts, but little is known about this process in higher eukaryotes.
How to study
2 、The movement patterns differed dramatically between the two stages.
3 、Chromosome movements included rotations of the entire chromatin and movements of individual chromosome segments
❖ slower-paced movements of other chromosome segments mostly located inside the chromatin mass
1、Three chromosome movement classes observed in a group of five zygotene meiocytes: rotational movements of the entire chromatin (yellow lines
(C) A pachytene nucleus overlaid with trajectories of chromosome marks shown every 60 s for 240 s. Green and blue mark the chromosome end and an interstitial knob, respectively, of a chromosome arm, which exhibits long-distance sweeping movements. Red: a chromosome loop whose both ends are embedded in the chromatin mass; cyan: a stationary chromosome region on the periphery of the chromatin mass; magenta: a free, fast moving chromosome end.
Pห้องสมุดไป่ตู้chytene Chromosome Movements
(A) Rotational movements of the entire chromatin in a pachytene nucleus. Yellow lines mark chromatin mass edges.
(B) Cumulative tracks from A after 570 s, but without chromatin shown.
Three Chromosome Movement Classes in Zygotene
❖ coordinated rotational movements of the entire chromatin
❖ rapid, short-distance oscillations of small individual chromosome segments extending from the main chromatin mass into the nuclear space
Why to research?
❖ The ability of chromosomes to move across the nuclear space is essential for the reorganization of the nucleus that takes place in early meiotic prophase.
❖ Difficulty :culture isolated live meiocytes of multicellular eukaryotes
❖ New way: observe live meiocytes inside intact anthers
result
1、maize chromosomes exhibited extremely dynamic and complex motility in zygonema and pachynema
rapid, short-distance movements of small chromosome segments (blue and green), slower-paced movements of chromosome segments inside the chromatin mass (red). 2、 Cumulative tracks marking rotational movements of the entire chromatin in the two meiocytes traced in A after 145 s. Starting and ending positions of the rotating chromatin in the nuclei from B and D. 3、 Cumulative tracks of the small chromosome segments tracked in the two meiocytes in A after 145 s
4 、Chromosome motility was coincident with dynamic deformations of the nuclear envelope
5 、 Both, chromosome and nuclear envelope motility depended on actin microfilaments as well as tubulin
❖ Chromosome dynamics of prophase I have been studied in budding and fission yeasts, but little is known about this process in higher eukaryotes.
How to study
2 、The movement patterns differed dramatically between the two stages.
3 、Chromosome movements included rotations of the entire chromatin and movements of individual chromosome segments
❖ slower-paced movements of other chromosome segments mostly located inside the chromatin mass
1、Three chromosome movement classes observed in a group of five zygotene meiocytes: rotational movements of the entire chromatin (yellow lines
(C) A pachytene nucleus overlaid with trajectories of chromosome marks shown every 60 s for 240 s. Green and blue mark the chromosome end and an interstitial knob, respectively, of a chromosome arm, which exhibits long-distance sweeping movements. Red: a chromosome loop whose both ends are embedded in the chromatin mass; cyan: a stationary chromosome region on the periphery of the chromatin mass; magenta: a free, fast moving chromosome end.
Pห้องสมุดไป่ตู้chytene Chromosome Movements
(A) Rotational movements of the entire chromatin in a pachytene nucleus. Yellow lines mark chromatin mass edges.
(B) Cumulative tracks from A after 570 s, but without chromatin shown.
Three Chromosome Movement Classes in Zygotene
❖ coordinated rotational movements of the entire chromatin
❖ rapid, short-distance oscillations of small individual chromosome segments extending from the main chromatin mass into the nuclear space
Why to research?
❖ The ability of chromosomes to move across the nuclear space is essential for the reorganization of the nucleus that takes place in early meiotic prophase.
❖ Difficulty :culture isolated live meiocytes of multicellular eukaryotes
❖ New way: observe live meiocytes inside intact anthers
result
1、maize chromosomes exhibited extremely dynamic and complex motility in zygonema and pachynema