海报(poster)模板(英文)

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Figure 2: Tyrosine fluorescence signal of Aβ42 was quenched in the presence of TiO2 nanoparticles.
References
Figure 3: Tyrosine fluorescence signal of Aβ42 is slightly quenched in the presence of SiO 2 , ZrO 2 , CeO 2 , C60, and C70 nanoparticles.
Abstract
We observed Aβ fibril formation in the presence of six kinds of nanoparticles and found that only TiO2 nanoparticles can promote Aβ fibrillation by shortening nucleation process.
TiO2 nanoparticles promote β-amyloid fibrillation in vitro
Wei-hui Wu, Xun Sun
Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology , Department of Chemistry, Tsinghua University, Beijing 100084, China
Results
Introduction
With more than 20 million cases worldwide, Alzheimer’s disease (AD) is the most common neurodegenerative disease.The central hypothesis for the cause of AD is the amyloid hypothesis, which states that β-amyloid (Aβ) peptide plays a key role inthe pathogenesis of the disease.So far, genetic factors have been mainly considered in the studies of the pathogenesis of AD. Here we show that nanoparticles may be one of the environmental factors causing AD.
Figure 1: Kinetics of Abfibrillation presence of different nanoparticles.
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Figure 4: Aβfibrillation in the presence of TiO2 nanoparti-cles.
Materials and methods
1
Conclusion
(1) TiO2 nanoparticles promote Aβ42 fibrillation by ThT assay (2) TiO2 nanoparticles can adsorb Aβ42 monomers on the surface of nanoparticles (3) SiO2, ZrO2, CeO2, C60, and C70 nanoparticles can slightly adsorb Aβ42 monomers on the surface of nanoparticles (4) TiO2 nanoparticles can promote Aβ42 fibrillation even at a very low concentration
Materials.Aβ42 was purchased from R-peptide Company (USA).TiO2 nanoparticles of 20 nm are a gift from Dr. R. Zong. SiO2particles of 15 nm, ZrO2 particles of 20 nm and CeO2 particles of 16 nm were provided by W. Zhou. Fullerenes C60 (purity > 99.5%) and C70(70% C70, 17% C60) were purchased from Wuda three-dimensioncarbon company. Sample preparation.Aβ42 was dissolved to 2mg/ ml in HFIP, incubated overnight at RT and stored at-20℃in HFIP. The HFIP was evaporated off by a speed vac before using. Fibrillation experiments.Aβ42 fibrillation was studied at 37℃in the absence or presence of nanoparticles in Eppendorf tubes. To further study TiO2 nanoparticle effects, TiO2 at different concentrations (0.0001, 0.001, 0.01, and 0.1 mg/ml, respectively) was incubated with Aβ42. Thioflavin T (ThT) (10lM) was added. Transmission electron microscopy (TEM). Fluorescence titrations.