血液细胞分析仪原理及应用

۩ 直方图是由测量通过感应区的每个细胞脉
二 、
冲累积得到的，根据库尔特原理可以在计
血
细
数的同时进行分析测量。
胞
计
数
检
测
原
理
Relationship between histogram and pulse signal
二 、 血 细 胞 计 数 检 测 原 理
۩ Red blood cell
۩ Biconcave disc Seedless
۩ Red blood cell diameter between 6 ~ 9 m, an average of 7.2 M.
۩ The number of red blood cells of the adult male was (4.6 ~ 6.2) * 1012 /L
二
size of the particles and the number of
、 血
the particles.
细 胞
计
数
检
测
原
理
仪器是如何进行细胞分群的呢？
۩ 仪器除给出细胞数据结果外，同时提供出 细胞体积分布图形，这些可以表示出细胞 群体分布情况的图形被称为直方图
۩ 它可以显示出某一特定细胞群的平均细胞 体积、细胞分布情况和是否存在明显的异 常细胞群
normal persons was (10 ~ 4) * 109 /L
۩ Platelet
۩ Both sides of the micro concave disk, when stimulated pseudopodia
۩ The normal platelet value (100 ~ 300) * 109 /L, diameter 2 ~ 4 µm.
۩ The number of red blood cells of the adult female was (5.4 ~ 4.2) * 1012 /L
۩ White blood cell ۩ Nuclear sphere ۩ The diameter is 7 ~ 25 M. ۩ The number of white blood cells in
through the hole, and the electric
resistance of the two electrode is
changed, and the potential pulse is
generated. The size and frequency of
the pulse signal is proportional to the
۩ Hemoglobin (hemoglobin or heme) ۩ Hemoglobin is present in red blood
cells and is a kind of protein containing iron. ۩ Adult male is 120 ~ 160g/L ۩ Adult female is 110 ~ 150g/L
۩ Platelet PLT ۩ Hemoglobin HGB
一、The development history of
blood cell analyzer
۩ In 1956, the American scientist W.H.Coulter patented technology for the use of the resistance method to count the number of particles in the blood cell count to be successful.
二 、 血 细 胞 计 数 检 测 原 理
Coulter principle
۩ When the electrolyte is suspended in the
electrolyte, the same volume of
Leabharlann Baidu
electrolyte is replaced by the electrolyte
۩ The principle is based on the nature of the non conduction of blood cells, the blood cell count and the volume of blood cells were detected by the blood cell count and volume measurement. The method is called the resistance method or the Kurt principle.
Principle and application of blood cell analyzer (血液细胞分析仪原理及应用)
۩ Human physiological and pathological changes, often caused by changes in blood components, so that a timely understanding of the changes in the blood component, can provide a diagnosis and treatment of diseases of the important basis for doctors.
Measurement parameters of blood cell analyzer
۩ Red blood cell RBC
۩ White blood cell WBC
۩ Platelet PLT ۩ Hemoglobin HGB
۩ Red blood cell RBC
۩ White blood cell WBC
۩ 国外厂商：
۩ 日本SYSMEX ۩ 美国beckman-
coulter ۩ 日本KOHDEN ۩ 美国DANAM ۩ 瑞典BOULE ۩ 美国BAYER ۩ 美国ABBOTT
۩ 国内厂商：
۩ 南昌特康 ۩ 南昌百特 ۩ 深圳迈瑞 ۩ 桂林百利特
1. 库尔特计数原理
۩ 库尔特原理（coulter principle） ：