低压氧舱模拟急性高原缺氧动物模型建立的初探

低压氧舱模拟急性高原缺氧动物模型建立的初探

马慧萍;吴金华;高荣敏;李琳;樊鹏程;景临林;贾正平

【期刊名称】《解放军药学学报》

【年(卷),期】2013(029)004

【摘要】目的模拟高原低压低氧环境建立小鼠急性缺氧模型,探讨不同缺氧时间对小鼠缺氧损伤程度的影响.方法采用国产低压低氧动物实验舱模拟海拔6000m高原缺氧环境,观察6h、12 h、24h、36 h、2d、3d、5d、7d不同缺氧时间小鼠组织病理学变化情况,并测定血浆LD、LDH和4种ATP酶的变化规律.结果 LD、LDH、T-AOC和ATP酶随缺氧时间的延长而呈波浪式变化,6h后可见组织形态发生明显改变,LD显著升高,T-AOC显著下降,12 h后LDH显著升高,ATP酶显著下降,说明在此条件下缺氧6～12h即可复制出急性高原病动物模型.结论该模型为下一步研究急性高原病发病机制提供了有利条件,在高原病防治研究中具有重要意义.%Objective To establish an animal model of acute mountain sickness using a decompression chamber and to investigate the effect of different hypoxic durations on the injury degree of experimental mice.Methods Rats were housed in a decompression chamber and exposed to simulated high altitude of 6000 m for 6 h,12 h,24 h,36 h,2 d,3 d,5 d and 7 d respectively.Pathological changes,the serum LD content,LDH activity and ATPase activity were examined respectively.Results The serum LD content,LDH activity and ATPase activity changed with time in a wave-like manner.Pathological changes of heart tissues were observed after 6 h.The LD content was increased while T-AOC was decreased significantly.After 12

h,the LDH activity was increased while AT-Pase activity was decreased.Conclusion An animal model of acute mountain sickness can be established within 6 to 12 h,which facilitates the mechanism study and prevention and treatment of acute mountain sickness.

【总页数】4页(P301-304)

【作者】马慧萍;吴金华;高荣敏;李琳;樊鹏程;景临林;贾正平

【作者单位】730050甘肃兰州,兰州军区兰州总医院药材科全军高原环境损伤防治重点实验室;730050甘肃兰州,兰州军区兰州总医院药材科全军高原环境损伤防治重点实验室;730050甘肃兰州,兰州军区兰州总医院药材科全军高原环境损伤防治重点实验室;730050甘肃兰州,兰州军区兰州总医院药材科全军高原环境损伤防治重点实验室;730050甘肃兰州,兰州军区兰州总医院药材科全军高原环境损伤防治重点实验室;730050甘肃兰州,兰州军区兰州总医院药材科全军高原环境损伤防治重点实验室;730050甘肃兰州,兰州军区兰州总医院药材科全军高原环境损伤防治重点实验室

【正文语种】中文

【中图分类】R965

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