山东师范大学生物化学(723)2005--2018年考研真题试卷

5R L!l R fl;9( 8~
~ 'f1A~f "*IJ
ifJ,tt-ij; JJ *t J.a- ;~-+j;I 1;f,,1, JJ1t~ ? ( 2 0::0'-)
~A
1/Vo
~B
1/VO
1/ISJ
1/[S]
v), T A, B, C ~ :0"IJIJ
~ 6ry 1f- EJ J0 ii.~iJf~ JW ,-it'iJL
JlJ*J}ijlm:::k~
@!±1iJfJt:~A~~t\t\HJR
i'±~~DJI[: 1. *iJ\~~ 3 il'tA:~ (;Ji.;~t 2. *~H.lhi\~~,
I£!P:mJ:Jl:j3CJ~o
12 't/J\~), ~:5t150:5t;
, ~{:E~iJ\~~J: , /G~1:EiJ\~J:Wt:M;
_" i1~Ji2 (:& Ij'Ji2 30 71-, jt 60 71- )
L "iJJ1J,5fU}tHlif:~tlFpll-Bi~7J~:t, Native-PAGE 7J~ti, SDS-PAGE
,m*
, ~ m~Urr7J1ti3fOi±.tPJ1§,~\,¥7J~ti:l:5]"iJJ~U JEEXit~~ 8 JJn?:tr';',
6" &~:5]Uj~
" fsJ~Ji2 (4lj/j'Ji215 71-, 6071-)
1" faji& Watson-Crick DNA .xxJ~hf£;fl~~ ,¢.l:RJti±.tPJ'¥f.l.x.o
mr 2" faji&Jtf~ mRNA 1*~ji ~~.Ll:~:bQ
0
8}J 't !f11]-IJIJ JLu}J~# -*)~1f-EJ J0 ii.Jif-iJf ;£-? f;"j i£.it~# 7J
;£-8~ ~;f..~ JW c ( 2 0:0")
Porous beads
Glass plastic column
~B 01 different prc!eins ):1
w 3" ~m~1*~~~W~~~~~~.~?
4, it~ bt~M~i*1L z, M-CoA 1:f~$xR~~?JTfI¥.f ~ f!~:fi IJ,R~4:t~~~~ ~m7f ~
C02 3fO H20 1iiJT~1~ ~ f!~';' 0 (f!~';' it~ IJ, ATP A ifLflL)
x..? m il­
JL I
~ f=.J J9f f!}) j»...f~ ~.ff 10f1:-1h ~ ~
11 ;f:~,;t f!}) j»".ft :11:] 8~ fJ.ff
DJJGJtt? 1s,iA)!) iJP.1t,-.ft~6~ 3-*1;;K -i~ fJ 1t 2.? (2 0 1J'- )
m.Ej mit.Ll:~~!MZ~lu JE ffiz ra], EX ~ !MZ~YlU JE ~ it it~ ffiz l'aJ
- 1+,
A1t~?
2, Aft~~tiJfn;tlt-f~¥119 Bi,
IE] Bi:it11T western, northern ~ southern
~3t11- ffT? fPIf~ 'f~ (£ r 1R fI¥.f ~
it 0 ( 2 0 1J'- )
Adenine: thymine
4 , A,B ff.Jj [ill 1Fra Baidu bibliotek- 11Ij JL
5/qz..-.
'r1A~r7
/-[f'0 ~ 'F­ -
~
;f.e,r ~f)I1J
8~ !
Lineweaver~Burk db t~ ( xx.. 1f';
), t~
1'Jn:..1,X,/,
r:p ~;tIt - f~ ~3t ~!MZ ~p "iJJ:0:iU
~~~n§~?W#m(£rfl¥.f~IE]Bi:it1ff~#EX-#~3t~!MZ7J~.~
?
* * * tlJ yf!J m
@J±1lJf9'1:1:A*~iJ\iJ\RlJI
: ].
2.
3.
** ** * ** * ** ** **** * **** ** *** * *** *** ~ ** ***
"'-t--"::::::'Small molecules
6
.,;
·I·~·
",;fjP
t6
..­.:
Tube to collect protein
Specific protein
Soluble ligand added
Ugand ./"
immobilizea
on inSOluble
support and
** ** **** * **** ** ** ** * *** **** * * ** *** *** * *
-" Mf-tiliiIT (4i/j\;~~511-, 3011-)
L R.t1Jt-iZoo
2" ~¥:ttfJ~7J
3" 71-r1t1B
4, %~~1ffl:q:
5" pg~M~Jl£~~~ il1*
packed in
glass column
W "'---::"proteins pass
~/
straight through
W
Specific protein elutes oft bound to soluble ligand
~c
Positively
charged
beads
Glass
column
Mixture of proteins
POSitively charged proteins
Buffer
f---""-~-"
wi & t+Y )O~
Negatively cnarged
r+\\..j:I
~0
proteins ~--------_ _
a~~0'~~t0r?r~'
2. M~~~#~~~~?~~~~~~, ~~#~~~~~JL~
-k:r10f1iitr1I*. 8~ ? (2 0 ~ )
r 3. i,f.:1i r9 -%r, :1i -1t !"8J "ffJ A~4t, it~!t 4'-8~ ~ltL I!J A E;
T 1f3 A ~4t 6~ I!J -iF, -itq£ H~ r 6~ fJ i\, 4'- tI:: G E; C @]C'.,xt 8t 8~