内毒素检测
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说明书1份1份
封板膜2片（48）2片（96）
密封袋1个1个
酶标包被板1×481×962-8℃保存标准品：0.9Eu/L0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液 1.5ml×1瓶 1.5ml×1瓶2-8℃保存酶标试剂 3 ml×1瓶 6 ml×1瓶2-8℃保存样品稀释液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂A液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂B液 3 ml×1瓶 6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶
样本处理及要求：
1.血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。仔细收集上
清，保存过程中如出现沉淀，应再次离心。
2.血浆：应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心
20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。
3.尿液：用无菌管收集，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程
中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。
4.细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/
分）。仔细收集上清。检测细胞内的成份时，用PBS（PH7.2-7.4）稀释细胞悬液，细胞
RD
Drug Names
Generic Name：Mouse endotoxin(ET)ELISA Kit.
Purpose
This kit allows for the determination of ET concentrations in Mouse serum, blood plasma, and other biological fluids.
Principle of the assay
T he kit assay Mouse ET level in the sample，use Purified Mouse ET antibody to coat microtiter plate wells, make solid-phase antibody, then add ET to wells,Combined antibody which With HRP labeled goat anti-mouse become antibody - antigen - enzyme-antibody complex, after washing Completely,Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ET in the samples is then determined by comparing the O.D. of the samples to the standard curve.
User manual11
Closure plate membrane22
Sealed bags11
Microelisa stripplate112-8℃Standard：0.9Eu/L0.5ml×1 bottle0.5ml×1 bottle2-8℃Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle2-8℃HRP-Conjugate reagent3ml×1 bottle6ml×1 bottle2-8℃Sample diluent3ml×1 bottle6ml×1 bottle2-8℃Chromogen Solution A3ml×1 bottle6ml×1 bottle2-8℃Chromogen Solution B3ml×1 bottle6ml×1 bottle2-8℃Stop Solution3ml×1 bottle6ml×1 bottle2-8℃
wash solution （20ml×20 fold）
×1bottle
（20ml×30 fold）
×1bottle
2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of
2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate or as an anticoagulant,mix 10-20 mins ,centrifugation
20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m.
remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container,
centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）,Rapidly
frozen with liquid nitrogen,maintain samples at2-8℃after melting,add PBS（PH7.4）,