【相关性分析何首乌肝毒性成分】

Journal of Hazardous Materials 299(2015)249–259

Contents lists available at ScienceDirect

Journal of Hazardous

Materials

j o u r n a l h o m e p a g e :w w w.e l s e v i e r.c o m /l o c a t e /j h a z m a

t

A novel method to analyze hepatotoxic components in Polygonum multiflorum using ultra-performance liquid

chromatography-quadrupole time-of-flight mass spectrometry

Longfei Lin a ,Hongmei Lin a ,Miao Zhang a ,Boran Ni b ,Xingbin Yin a ,Changhai Qu c ,Jian Ni a ,∗

a

School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing,China b

School of Basic Medical Science,Beijing University of Chinese Medicine,Beijing,China c

Modern Research Center for TCM,Beijing University of Chinese Medicine,Beijing,China

h i g h l i g h t s

•Investigated the hepatotoxicity of

Polygonum multiflorum and its con-stituents.

•The run time of each sample was 25min by UPLC-Q-TOF/MS.

•Used an analytical method based on Progenesis QI and Makerlynx XS soft-ware.

•Identified 9potential hepatotoxic components in P.multiflorum.

g r a p h i c a l

a b s t r a c

t

a r t i c l e

i n f o

Article history:

Received 17March 2015

Received in revised form 21May 2015Accepted 6June 2015

Available online 9June 2015

Keywords:

Polygonum multiflorum

Polygonum multiflorum Praeparata Hepatotoxicity Toxicity

UPLC-Q-TOF/MS

a b s t r a c t

Polygonum multiflorum ,called Heshouwu in China,is a traditional Chinese medicine used to treat var-ious diseases .However,the administration of P.multiflorum (PM)and P.multiflorum Praeparata (PMP)causes numerous adverse effects.This study sought to analyze the toxic components of PM using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS),and their hepatotoxicity in L02human liver cells.Toxicity was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT),lactate dehydrogenase (LDH)leakage,and liver enzyme secretion (aspartate aminotransferase [AST]and alanine aminotransferase [ALT])assays.Furthermore,UPLC-Q-TOF/MS,Progenesis QI,and Makerlynx XS software analyses were used to differentiate extracts and analyze the toxic components.The order of toxicity was P.multiflorum ethanol extract (PME)>P.multiflorum water extract (PMW)>P.multiflorum Praeparata ethanol extract (PMPE)>P.multiflorum Praeparata water extract (PMPW),which was determined by MTT assay,LDH leakage,and liver enzyme secretion levels.The analysis methods suggest that PM toxicity may be associated with anthraquinone,emodin-O -(malonyl)-hex,emodin-O -glc,emodin,emodin-8-O -glc,emodin-O -(acetyl)-hex,and emodin-O -hex-sulphate.The toxic mechanisms of these components require further study.

©2015Elsevier B.V.All rights reserved.

∗Corresponding author.Fax:+861084738607.E-mail address:njtcm@ (J.Ni).

1.Introduction

Polygonum multiflorum is a popular traditional Chinese medicine used in many patent medicines and prescriptions.Recent stud-ies showed that PM has antioxidant activity,due to its flavonoid

/10.1016/j.jhazmat.2015.06.0140304-3894/©2015Elsevier B.V.All rights reserved.