甘露聚糖结合凝集素在复发性流产患者血清中表达水平及意义

文章编号:1003-6946(2010)11-833-03

甘露聚糖结合凝集素在复发性流产患者血清中表达水平及意义

邹璐杰,朱付凡

(中南大学湘雅二医院,湖南长沙410011)

=摘要> 目的:了解甘露聚糖结合凝集素(MBL)在不明原因复发性流产患者血清中的表达水平,探讨M BL与复发性流产免疫感染的关系。方法:应用酶联免疫吸附实验对比检测MBL在55例不明原因复发性流产患者(研究组)及22例正常早孕妇女(对照组)血清中的表达水平,分析MBL血清水平与患者免疫抗体表达、病原体感染的相关性。结果:血清M BL水平在复发性流产患者及正常早期妊娠妇女中呈非正态分布。研究组中,血清M BL水平下降(<550ng/m l)者占70.9%(39/55),明显高于对照组45.5%(10/22),差异有统计学意义(P<0.05)。研究组中免疫抗体阳性患者血清MBL水平下降(<550ng/m l)率68.8%,与抗体阴性患者相比差异无统计学意义(P>0.05)。研究组中亚临床感染患者血清MBL水平下降(<550ng/m l)率80.8%略高于非感染患者的M BL水平下降率62.1%(18/29),但两组相比较差异无统计学意义(P> 0.05);亚临床感染组患者MBL缺陷率(<200ng/m l)23.1%,显著高于非感染组患者的MBL水平缺陷率3.45%(P<0.05)。结论:¹血清MBL水平在正常早孕妇女及复发性流产患者中均变化范围较大,复发性流产患者血清MBL水平表达偏低。º复发性流产患者血清MBL下降率显著高于正常早孕妇女,血清M BL水平缺陷导致病原体易感性增加,可能是复发性流产的危险因素之一。

=关键词>甘露聚糖结合凝集素;复发性流产;感染;免疫

中图分类号:R714121文献标识码:A

Exp r ession of Seru m M BL i n Recurr ent Abortion Patients and its S ign if-i cance

Z OU Luji e,ZHU Fuf a n

(The Secend X iangya Hosp it a l o f Cen tra l Sou t h Unive rsity,Changsha Hunan410011,Ch i n a)

=Abstract> O bjective:To investiga t e the exp ression o f seru m m annan-b ind ing lectin(MBL)in une xp lained recurrent abortion pa ti e nts and approach the re l a ti o nship be t w een MBL and m i m un it y in f ecti o n o f re curren t a-bortion.Me thods:The concen tra tions o f se rum MBL in55unexp lained re curren t abo rtion patients(exper-i men t a l g roup)and22hea lthy p regnan t women(con tro l g roup)we re measured by e nzy me linked m i m u-nosorben t assay(EL I SA)k i.t The re la tionsh ip be t ween seru m leve l o fMBL and expre ssion of m i mun it y ant-i bod ies,pa t hogen infections was ana lyzed.Results:The Exp ression o f se rum MBL w as in nonno r ma l d istr-i bution i n recurren t abo rtion pa tien t s and hea lthy contro ls.Th irty-n ine women in experm i enta l group(70.9%, 39/55)had MBL conce ntrations l e ss t han550ng/m l in co m parison w ith ten i n con tro l g roup(45.5%, 10/22).There was si g n ificant d iff e rence be t ween t w o g roups(P<0.05).I n expe rm i en t a l g roup,t he de t ec-tion ra t e o fMB L concen tra ti o ns l e ss than550ng/m L in m i mun it y an ti b ody po sitive g roup was68.8%in com-parison w it h73.9%in m i m un ity an ti b ody neg tive gro up.There was no sign ifican t d iff erence be t w een t wo groups(P﹥0.05).The decrea se rate o f MBL leve l(<550ng/m l)i n subclin ica l in f ec tion pa ti e n ts was80. 8%,w hich was a little h i g he r than t ha t in non-subclin ica l in f ec tion pa tien ts(62.1%,18/29).There was no sign ifican t diff e rence be t w een t wo group s(P﹥0.05).The de fi c i e ncy ra t e o fMBL(<200ng/m l)in subclin-i ca l in f ection pa ti e n ts w as23.1%,wh ich was si g n ificant h i g he r t han t ha t in non-subc linica l in f ection pa tien ts (3.45%,P<0.05).Conc l us i ons:¹Se rum MB L leve l va ries in a la rge sca l e in bo t h recurren t abortion pa-tien t s and hea lt hy con tro ls.The exp ressi o n o f se rum MB L in recurren t abo rti o n pa ti e nts is lowe r.ºThe de-crease ra t e o f MBL leve l in recu rrent abortion pa tient is signifi c antly h igher t han t ha t in hea lt hy con tro ls.S er-

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