紫甘薯花色苷的提取、纯化及肝损伤保护功能的研究
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III
Based on the above process, a systematic production process was obtained. Under this condition, the colour value of PSPPs reached 27.7.
The hepa-protective effect in vivo: using CCl4 to ind uce acute and chemical liver injury model, contrasting the ALT and AST indictors in serum among the control group, PSPPs low, medium and high dose group and the model group, the conclusion has been reached that PSPPs had some preventive effects on acute and chemical liver injury. PSPPs medium and high dose group could significantly reduce the level of Alanine Transaminase (ALT), and the effect of medium dose group (P=0.011) was better than high dose group (P=0.021). The optimal dose can be speculated is the medium dose of 455mg/Kg, and high dose may have some side effects. Compared with model group, the inhibition of PSPPs high dose on Aspartate Aminotransferase (AST) was significantly different while the surpression of PSPPs low and medium dose was limited.
在上述提取纯化工艺基础上，得到了一套系统的制备紫甘薯花色苷浓缩液的生 产工艺流程。在此工艺条件下制备的紫甘薯花色苷色价可达到 27.7。
肝损伤保护功能的体内研究：采用 CCl4 诱导产生急性化学性肝损伤模型，通过 正常对照组、紫甘薯花色苷低、中、高剂量组与 CCl4 肝损伤模型组血清中 ALT 和 AST 指标的比较，得出如下结论：紫甘薯花色苷对急性化学性肝损伤有一定的预防 作用，其中中 剂 量 和 高 剂 量 的 紫 甘 薯 花 色 苷 可 以 显 著 降 低 血 清 中 谷 丙 转 氨 酶 （ ALT）的水平而且中剂量的保肝效果（P=0.011）要优于高剂量（ P=0.021）， 可推测出最佳剂量为中剂量，即 为 455mg/Kg，高剂量可能存在一定的毒副作用； 与模型组相比，高剂量的紫甘薯花色苷对血清谷草转氨酶（ AST） 水平的抑制 作用具有极显著性差异，而低剂量和中剂量的抑制能力有限。
The crude anthocyanin pigments purified by two kinds of Macro-porous resin (D-101 and AB-8) were investigated. The results indicated that the AB-8 type Macro-porous resin was superior for the purification of PSPPs. Process parameters were that the pH of sample solution was 2, sample flow rate was 1.0 mL / min, the sample concentration was 57.0 µg/mL, elution flow rate was 1.0 mL / min, eluent was 70% ethanol.
Keyword ： Purple sweet potato;anthocyanins;extraction;purification;hepa-protective effect
First, the extraction of purple sweet potato pigments (PSPPs) were done under the same conditions, except for the only difference that one of the solvent was 2% citric acid solution while the other was the citric acid acidified ethanol with a pH of 3. By the comparison of colour value and sugar content it can be drawn that the citric acid water extraction outweighs the citric acid acidified ethanol. Based on the single factor and orthogonal tests , optimum data were as follows extraction temperature was 50℃, extraction time was 1h for 2 times, the concentration of citric acid was 4%(m/v), solid to liquid ratio was 1:6. Under such conditions, the anthocyanins yield from purple sweet potato was 2.843mg/g.
II
Abstract
Anthocynidin is a kind of water-soluble pigments which are widely indwelled in plants, belonging to flavonoids. In the natural state, it is often combined with sugars through glycosidic bond to form glycosides, known as Anthocynins.Owing to its safety, bright natural colour, no special smell and oxidantion resistance, protection of liver function and other health care functions，it has drawn nation wide attention and been put into industrial production. Characterized by high output, easy to cultivate and high content of anthocyanins, purple sweet potato becomes an ideal way to get natural anthocyanins, therefore, the development and use of anthocyanins from purple sweet potato become a major research focus.
Purple sweet potato, provided by Weihai Yijingyuan Fruit and Vegetable Co., Ltd., as the experimental material in this subjuct, the extraction and purification and hepa-protective effect were researched.
采用 D-101 和 AB-8 两种大孔树脂对花色苷粗提液进行纯化，通过色价、含糖量 和花色苷浓度的比较筛选出一种性能较好的大孔树脂。结果显示：AB-8 大孔树脂对 紫甘薯花色苷的纯化效果要优于 D-101 大孔树脂。纯化工艺参数为上样液的 pH 值为 2，上样流速为 1.0 mL/min ，上样浓度为 57.0µg/mL，洗脱流速为 1.0 mL/min ，洗 脱液为 70%乙醇。
烟台大学 硕士学位论文 紫甘薯花色苷的提取、纯化及肝损伤保护功能的研究 StudyontheExtractionandPurificationandHepaprotectiveeffectofanthocyaninsfromPurpleSweetPotato 姓名：李玲 申请学位级别：硕士 专业：农学；食品加工与安全 指导教师：刘玉田；申京宇
I
对氧化损伤肝细胞的保护作用：花 色 苷 的 中 剂 量 组 和 高 剂 量 组 肝 细 胞 谷 胱 甘 肽过氧化物酶（ GSH- Px）活性高于氧化损伤组，差别具有统计学意义 (P<0.01)； 中剂量组和高剂量组与氧化损伤组的 超氧化物歧化酶（SOD） 活力具有显著性 差异(P<0.01)；紫甘薯花色苷中剂量组和高剂量组的 丙二醛（MDA）水平显著 低于 H2O2 氧化损伤组，差异具有统计学意义(P<0.01)。 关键词：紫甘薯；花色苷；提取；纯化；肝损伤保护功能
The protection of hepatocytes injured by H2O2: the Glutathione Peroxidase (GSH-Px) activity of PSPPs medium and high dose group was higher than the oxidative damage group, and the difference was statistically significant (P<0.01); the Superoxide Dismutase (SOD) activity of midium and high dose group and the model group had significant difference (P<0.01); the Maleic Dialdehyde (MDA) level of PSPPs medium and high dose group was lower than the model group, and the difference was statistically significant (P<0.01).
2011-04
摘要
花色素是一类广泛存在于自然界植物中的水溶性色素，属于类黄酮称为花色苷。因其安全、色泽鲜 亮自然、无特殊气味兼具抗氧化、保护肝脏及其它保健功能，受到广泛关注，现已 投入工业化生产。紫甘薯产量高，易栽培，花色苷含量高，成为获取天然花色苷的 理想途径，因此，紫甘薯中花色苷的开发和利用成为一大研究热点。
本课题以威海亿晶原果蔬有限公司的紫甘薯为实验材料，对其花色苷的提取、 纯化工艺和肝损伤保护功能进行了研究。
首先，采用同样的工艺流程提取紫甘薯花色苷，仅以提取溶剂为变量因素时， 通过色价和含糖量的比较可以得出：柠檬酸水浸提法的提取效果要优于柠檬酸酸化 乙醇浸提法。然后在单因素及正交试验的基础上确定了该品种紫甘薯花色苷提取的 最佳工艺参数：柠檬酸浓度为 4%，提取时间为 1h，提取温度为 50℃，料液比为 1:6， 提取次数为 2 次，在此条件下紫甘薯花色苷的得率为 2.843mg/g。
Based on the above process, a systematic production process was obtained. Under this condition, the colour value of PSPPs reached 27.7.
The hepa-protective effect in vivo: using CCl4 to ind uce acute and chemical liver injury model, contrasting the ALT and AST indictors in serum among the control group, PSPPs low, medium and high dose group and the model group, the conclusion has been reached that PSPPs had some preventive effects on acute and chemical liver injury. PSPPs medium and high dose group could significantly reduce the level of Alanine Transaminase (ALT), and the effect of medium dose group (P=0.011) was better than high dose group (P=0.021). The optimal dose can be speculated is the medium dose of 455mg/Kg, and high dose may have some side effects. Compared with model group, the inhibition of PSPPs high dose on Aspartate Aminotransferase (AST) was significantly different while the surpression of PSPPs low and medium dose was limited.
在上述提取纯化工艺基础上，得到了一套系统的制备紫甘薯花色苷浓缩液的生 产工艺流程。在此工艺条件下制备的紫甘薯花色苷色价可达到 27.7。
肝损伤保护功能的体内研究：采用 CCl4 诱导产生急性化学性肝损伤模型，通过 正常对照组、紫甘薯花色苷低、中、高剂量组与 CCl4 肝损伤模型组血清中 ALT 和 AST 指标的比较，得出如下结论：紫甘薯花色苷对急性化学性肝损伤有一定的预防 作用，其中中 剂 量 和 高 剂 量 的 紫 甘 薯 花 色 苷 可 以 显 著 降 低 血 清 中 谷 丙 转 氨 酶 （ ALT）的水平而且中剂量的保肝效果（P=0.011）要优于高剂量（ P=0.021）， 可推测出最佳剂量为中剂量，即 为 455mg/Kg，高剂量可能存在一定的毒副作用； 与模型组相比，高剂量的紫甘薯花色苷对血清谷草转氨酶（ AST） 水平的抑制 作用具有极显著性差异，而低剂量和中剂量的抑制能力有限。
The crude anthocyanin pigments purified by two kinds of Macro-porous resin (D-101 and AB-8) were investigated. The results indicated that the AB-8 type Macro-porous resin was superior for the purification of PSPPs. Process parameters were that the pH of sample solution was 2, sample flow rate was 1.0 mL / min, the sample concentration was 57.0 µg/mL, elution flow rate was 1.0 mL / min, eluent was 70% ethanol.
Keyword ： Purple sweet potato;anthocyanins;extraction;purification;hepa-protective effect
First, the extraction of purple sweet potato pigments (PSPPs) were done under the same conditions, except for the only difference that one of the solvent was 2% citric acid solution while the other was the citric acid acidified ethanol with a pH of 3. By the comparison of colour value and sugar content it can be drawn that the citric acid water extraction outweighs the citric acid acidified ethanol. Based on the single factor and orthogonal tests , optimum data were as follows extraction temperature was 50℃, extraction time was 1h for 2 times, the concentration of citric acid was 4%(m/v), solid to liquid ratio was 1:6. Under such conditions, the anthocyanins yield from purple sweet potato was 2.843mg/g.
II
Abstract
Anthocynidin is a kind of water-soluble pigments which are widely indwelled in plants, belonging to flavonoids. In the natural state, it is often combined with sugars through glycosidic bond to form glycosides, known as Anthocynins.Owing to its safety, bright natural colour, no special smell and oxidantion resistance, protection of liver function and other health care functions，it has drawn nation wide attention and been put into industrial production. Characterized by high output, easy to cultivate and high content of anthocyanins, purple sweet potato becomes an ideal way to get natural anthocyanins, therefore, the development and use of anthocyanins from purple sweet potato become a major research focus.
Purple sweet potato, provided by Weihai Yijingyuan Fruit and Vegetable Co., Ltd., as the experimental material in this subjuct, the extraction and purification and hepa-protective effect were researched.
采用 D-101 和 AB-8 两种大孔树脂对花色苷粗提液进行纯化，通过色价、含糖量 和花色苷浓度的比较筛选出一种性能较好的大孔树脂。结果显示：AB-8 大孔树脂对 紫甘薯花色苷的纯化效果要优于 D-101 大孔树脂。纯化工艺参数为上样液的 pH 值为 2，上样流速为 1.0 mL/min ，上样浓度为 57.0µg/mL，洗脱流速为 1.0 mL/min ，洗 脱液为 70%乙醇。
烟台大学 硕士学位论文 紫甘薯花色苷的提取、纯化及肝损伤保护功能的研究 StudyontheExtractionandPurificationandHepaprotectiveeffectofanthocyaninsfromPurpleSweetPotato 姓名：李玲 申请学位级别：硕士 专业：农学；食品加工与安全 指导教师：刘玉田；申京宇
I
对氧化损伤肝细胞的保护作用：花 色 苷 的 中 剂 量 组 和 高 剂 量 组 肝 细 胞 谷 胱 甘 肽过氧化物酶（ GSH- Px）活性高于氧化损伤组，差别具有统计学意义 (P<0.01)； 中剂量组和高剂量组与氧化损伤组的 超氧化物歧化酶（SOD） 活力具有显著性 差异(P<0.01)；紫甘薯花色苷中剂量组和高剂量组的 丙二醛（MDA）水平显著 低于 H2O2 氧化损伤组，差异具有统计学意义(P<0.01)。 关键词：紫甘薯；花色苷；提取；纯化；肝损伤保护功能
The protection of hepatocytes injured by H2O2: the Glutathione Peroxidase (GSH-Px) activity of PSPPs medium and high dose group was higher than the oxidative damage group, and the difference was statistically significant (P<0.01); the Superoxide Dismutase (SOD) activity of midium and high dose group and the model group had significant difference (P<0.01); the Maleic Dialdehyde (MDA) level of PSPPs medium and high dose group was lower than the model group, and the difference was statistically significant (P<0.01).
2011-04
摘要
花色素是一类广泛存在于自然界植物中的水溶性色素，属于类黄酮称为花色苷。因其安全、色泽鲜 亮自然、无特殊气味兼具抗氧化、保护肝脏及其它保健功能，受到广泛关注，现已 投入工业化生产。紫甘薯产量高，易栽培，花色苷含量高，成为获取天然花色苷的 理想途径，因此，紫甘薯中花色苷的开发和利用成为一大研究热点。
本课题以威海亿晶原果蔬有限公司的紫甘薯为实验材料，对其花色苷的提取、 纯化工艺和肝损伤保护功能进行了研究。
首先，采用同样的工艺流程提取紫甘薯花色苷，仅以提取溶剂为变量因素时， 通过色价和含糖量的比较可以得出：柠檬酸水浸提法的提取效果要优于柠檬酸酸化 乙醇浸提法。然后在单因素及正交试验的基础上确定了该品种紫甘薯花色苷提取的 最佳工艺参数：柠檬酸浓度为 4%，提取时间为 1h，提取温度为 50℃，料液比为 1:6， 提取次数为 2 次，在此条件下紫甘薯花色苷的得率为 2.843mg/g。