分子生物学简答题题目

1、如何理解结构决定功能？

1、Each allele has a different phenotype ,why(illustrate by example).

2、Conservation of exons and its application.

3、Chromosome walking and its application.

4、How to clone gene for genome?

5、利用cDNA克隆某基因（举例）

6、蛋白质组学研究中所用的方法及原理

7、RFLP可以作为一种遗传标记

8、限制性位点能否体现孟德尔遗传的特性

9、目前遗传研究中所用的4类遗传标记

10、人类基因组数目、蛋白质组的成员，为什么后者远多于前者？

11、人类线粒体基因组与酿酒酵母线粒体基因组间的异同

12、To illustrate the developmental control via globin.

13、动物可以从植物中得到营养的原因？

14、Unequal crossing-over can result in what results?

15、DNA fingerprinting.

16、The “adaper” is transfer RNA, why?

17、比较三种RNA的结构及功能

18、How to produce the cap and the poly(A) for eukaryotic mRNA?

19、What’s its function of poly (A) and how to use it in experiments？

20、5-FU抗癌机理

21、To compare the process of protein synthesis for prokaryotic and eukaryotic.

22、What is Tu-Ts cycle?

23、How to demonstrate that inhibiting one step in protein synthesis blocks the next step for kirromycin?

24、How to reveal the nature of the transfer reaction via the antibiotic puromycin?

25、How to form 48S complex?

26、How to inhibit the process of the protein synthesis at particular stages by using antibiotics?

27、How to process the 3' end and the the 5' end of a tRNA?

28、Inosine can pair with any of U, C, and A，why?

29、To illustrate missense suppressors completed with wild-type.

30、How to prevent random aggregation of proteins by chaperones?

31、Why they are named“hsp”?

32、The signal sequence provides what between the ribosomes and the membrane.

33、Explain the nuclear pores are used for both import and export of material.

34、The function and mechanism of ubiquitin?

35、What is its meaning for research ubiquitin?

36、How does rho factor work?

37、To illustrate control circuits can be designed to allow positive or negative control of induction or repression (with galactose、lactose、arabinose etc.，CRP protein has to be used).

38、The E.coli tryptophan operon is controlled by attenuation，describe its mechanism please.

39、Antisense RNA offers a powerful approach for turning off genes at will，give an example please.

40、What’s RNAi? Give an example please.

41、Try to explain phage lytic development proceeds by a regulatory cascade.

42、The cⅡand cⅢgenes are needed to establish lysogeny，why?

43、Cro binds to the same operators as repressor but with different affinities，which result in what result.

44、What determines the balance between lysogeny and the lytic cycle(in details)?

45、What is the vital function of opines for transformed plant cell and the bacterium.

46、What responsible for transferring T-DNA to an infected plant.

47、How to explain why Agrobacterium infection succeeds only on wounded plants？